CN113444272B - Gel film and preparation method of gel film sensor - Google Patents
Gel film and preparation method of gel film sensor Download PDFInfo
- Publication number
- CN113444272B CN113444272B CN202110633530.XA CN202110633530A CN113444272B CN 113444272 B CN113444272 B CN 113444272B CN 202110633530 A CN202110633530 A CN 202110633530A CN 113444272 B CN113444272 B CN 113444272B
- Authority
- CN
- China
- Prior art keywords
- gel
- intelligent
- gel film
- precursor solution
- asymmetric channel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/02—Use of particular materials as binders, particle coatings or suspension media therefor
- C09K11/025—Use of particular materials as binders, particle coatings or suspension media therefor non-luminescent particle coatings or suspension media
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/12—Agar-agar; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/16—Nitrogen-containing compounds
- C08K5/34—Heterocyclic compounds having nitrogen in the ring
- C08K5/3467—Heterocyclic compounds having nitrogen in the ring having more than two nitrogen atoms in the ring
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/10—Non-macromolecular compounds
- C09K2211/1018—Heterocyclic compounds
- C09K2211/1025—Heterocyclic compounds characterised by ligands
- C09K2211/1074—Heterocyclic compounds characterised by ligands containing more than three nitrogen atoms as heteroatoms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Abstract
The invention belongs to the field of biochemical sensors, and particularly relates to a gel film and a preparation method of the gel film sensor. The preparation method of the gel film comprises the following steps: the gel precursor solution is driven to move in the hollow asymmetric channel through pressure difference, the section area of the asymmetric channel is gradually increased along the radial direction, the moving direction of the gel precursor solution is from the smaller section side of the asymmetric channel to the larger section side of the asymmetric channel, the thickness of the gel precursor solution is gradually reduced in the moving process, and after the gel precursor solution is moved to a certain position, the gel precursor solution is solidified to form a gel membrane structure. By controlling the moving position of the gel precursor solution, a gel film structure of a target thickness is obtained. The preparation method has small gel dosage (microliter grade), and the gel utilization rate is close to 100%.
Description
Technical Field
The invention belongs to the field of biochemical sensors, and particularly relates to a gel film and a preparation method of the gel film sensor.
Background
Compared with homogeneous chemical sensors, the thin film sensor has the advantages of no pollution to a system to be measured, reusability, easy device formation and the like, and is popular among people. The thin film chemical sensor using fluorescence as a sensing signal has the advantages of high sensitivity, abundant detection signals, no reagent consumption, no need of a reference substance, capability of realizing remote detection and the like, and is rapidly developed in recent years. The photochemical stability of the fluorescent substance in the dissolved state is much higher than that of the conventional film, and the additional optical stability can be obtained by preparing the fluorescent film through gel mediation, so that the fluorescent film is widely concerned by researchers. The basis of the development of the gel film-based sensor is a high-performance sensitive film material. The sensitive gel film with excellent efficient creation performance has a basic effect in the process of developing a high-performance sensor.
In the prior art, most of gel film preparation processes with high uniformity depend on methods such as lifting, spin coating and tape casting, most of the processes depend on expensive professional equipment, the gel consumption is large (milliliter level), gel is wasted in the preparation process, and the gel utilization rate is not high. Moreover, the prior art is not easy to control the film thickness due to the property difference of the gel precursor solution when preparing the gel film structure with different components.
According to the traditional fluorescence sensor based on the gel film, one side of the gel film is a solid phase, the other side of the gel film is a gas phase, in the detection process, a liquid sample fills one side of the gas phase to generate a fluorescence signal, the requirement on the light transmittance of the sample is high, and other substances which interfere with the fluorescence in a sample system can generate adverse effects on the detection result.
Disclosure of Invention
In order to solve the technical problems, the invention provides a gel membrane and a preparation method of a gel membrane sensor, wherein a hydrogel material is driven to move in a hollow asymmetric channel through pressure difference, the form of the hydrogel material is changed (gradually thinned) in the moving process, and a gel membrane structure is formed under the action of surface tension.
The invention is realized by the following technical scheme:
a method of making a gel film, comprising: the gel precursor solution is driven to move in the hollow asymmetric channel through pressure difference, the section area of the asymmetric channel is gradually increased along the radial direction, the moving direction of the gel precursor solution is from the smaller section side of the asymmetric channel to the larger section side of the asymmetric channel, the thickness of the gel precursor solution is gradually reduced in the moving process, and after the gel precursor solution is moved to a certain position, the gel precursor solution is solidified to form a gel membrane structure.
In the above process, the gel precursor solution undergoes a morphological change (gradual thinning) during its movement, and a gel film structure is formed by the action of surface tension.
Further, by controlling the moving position of the gel precursor solution, a gel film structure of a target thickness is obtained.
Further, the gel precursor solution is driven by a pressure difference, in particular: applying pressure to the end of the asymmetric channel having the smaller cross-section using a gas or other phase that does not react with the gel precursor solution, thereby creating a pressure differential across the asymmetric channel.
Further, the section of the asymmetric channel comprises one of a circle, an ellipse and a polygon, and the radius range of the section is 0.01-5 cm; the length of the asymmetric channel is 0.1-10 cm.
Further, the volume of the gel precursor solution required to prepare each gel film structure is 0.1-1000 uL; in preparing the gel film structure, the speed of the gel precursor solution moving in the asymmetric channel is controlled to be 0.01-2mm per minute.
Further, the gel membrane structure is bounded by the peripheral asymmetric channels; the gel film structure has a thickness of 0.001-10 mm.
A method of making a gel film sensor, the method comprising: the method comprises the following steps that an intelligent hydrogel material containing an intelligent recognition factor is adopted, the intelligent hydrogel material is driven to move in a hollow asymmetric channel through pressure difference, the area of the section of the asymmetric channel is gradually increased along the radial direction, the moving direction of the intelligent hydrogel material is from the smaller side of the section of the asymmetric channel to the larger side of the section of the asymmetric channel, the shape and thickness of the intelligent hydrogel material are gradually reduced in the moving process, and after the intelligent hydrogel material is moved to a certain position, the intelligent hydrogel material is solidified to form a gel membrane structure containing the intelligent recognition factor, namely the gel membrane sensor is obtained;
the intelligent hydrogel material comprises: gel precursor solution and intelligent recognition factors capable of recognizing the target to be detected; the intelligent recognition factor is capable of undergoing a detectable transition in a physical property upon target stimulation.
In the method of manufacturing the gel film sensor:
driving the intelligent hydrogel material by pressure difference, in particular: and applying pressure to the end with the smaller section of the asymmetric channel by using gas or other phases which do not react with the intelligent hydrogel material, so as to generate pressure difference on two sides of the asymmetric channel.
The section of the asymmetric channel comprises one of a circle, an ellipse and a polygon; the radius of the section is 0.01-5cm, and the length is 0.1-10 cm.
The volume of the intelligent hydrogel material required for preparing a gel film structure containing the intelligent hydrogel material is 0.1-1000 uL; in the preparation of the gel film structure, the speed of the intelligent hydrogel material moving in the asymmetric channel is controlled to be 0.01-2mm per minute.
Said gel membrane structure being bounded by said asymmetric channels at the periphery; the gel film structure has a thickness of 0.001-10 mm.
Further, when the target to be detected is copper ions, in the intelligent hydrogel material, gelma solution is adopted as the gel precursor solution, and sodium fluorescein serving as a fluorescent molecule capable of identifying the copper ions is adopted as the intelligent identification factor; adding isooctyl p-dimethylaminobenzoate as a photoinitiator into the intelligent hydrogel material;
wherein the gelma solution is methacrylated gelatin, the adopted gelma solution has the mass percentage concentration of 5-15 percent, the concentration of fluorescent molecular fluorescein sodium is 0.5-10mM, and the mass percentage concentration of p-N, N-dimethylaminobenzoic acid isooctyl ester is 0.1-1 percent;
when the target to be detected is potassium ions, in the intelligent hydrogel material, the gel precursor solution adopts an agarose solution, the intelligent recognition factor adopts a nucleic acid aptamer capable of recognizing potassium ions, and the nucleic acid sequence is as follows: CGC GGG TTT TGG GTT TTG GGT TTT GGG G, respectively; and adding N-methyl porphyrin dipropionic acid capable of generating a fluorescence signal into the intelligent hydrogel material.
Wherein, the mass percentage concentration of the adopted agarose solution is 1-3 percent, the concentration of the aptamer is 0.5-2.5mM, and the concentration of the N-methylporphyrin dipropionic acid is 0.1-10 mM;
a gel film sensor prepared using the method, the gel film sensor comprising: the device comprises a hollow asymmetric channel and an intelligent gel film, wherein the periphery of the intelligent gel film is attached to the inner wall of the asymmetric channel; the intelligent gel film is formed after the gel precursor solution is solidified;
the cross-sectional area of the asymmetric channel gradually increases along the radial direction;
the intelligent gel film comprises an intelligent recognition factor capable of recognizing a target to be detected; the intelligent recognition factor is capable of undergoing a detectable transition in a physical property upon target stimulation.
Further, the cross section of the asymmetric channel comprises one of a circle, an ellipse, and a polygon; the radius range of the cross section is 0.01-5cm, and the length of the asymmetric channel is 0.1-10 cm;
the volume of the intelligent hydrogel material required for preparing each gel film sensor is 0.1-1000 uL; the thickness of the intelligent gel film in the gel film sensor is 0.001-10 mm.
The invention has the beneficial technical effects that:
the gel film preparation method provided by the invention has the advantages that the gel dosage is small (microliter level), and the gel utilization rate is close to 100%.
The equipment adopted by the gel film preparation method provided by the invention is low in price and easy to obtain, and the thickness of the gel film can be quickly and accurately regulated and controlled by regulating the position of the gel precursor solution.
The gel film structure related to the gel film sensor provided by the invention is an independent film structure, both sides of the film are gas phases, one side of the film is in contact with a liquid sample to generate a specific fluorescence signal, and the other side of the film is used for collecting the fluorescence signal in the detection process, so that the problem of interference on fluorescence optics in the sample is solved.
Drawings
FIG. 1A is a schematic diagram of a gel film preparation according to an embodiment of the present invention;
FIG. 1B is a schematic diagram of the operation of a gel film sensor in an embodiment of the present invention;
FIG. 2 is a schematic view of a gel film in an embodiment of the present invention;
FIG. 3A is a fluorescence diagram of the copper ion detection process in an embodiment of the present invention.
FIG. 3B is a diagram illustrating the detection effect according to the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
On the contrary, the invention is intended to cover alternatives, modifications, equivalents and alternatives which may be included within the spirit and scope of the invention as defined by the appended claims. Furthermore, in the following detailed description of the present invention, certain specific details are set forth in order to provide a better understanding of the present invention. It will be apparent to one skilled in the art that the present invention may be practiced without these specific details.
The present invention provides an embodiment of a method of making a gel film, the method comprising: the gel precursor solution is driven to move in the hollow asymmetric channel through pressure difference, the section area of the asymmetric channel is gradually increased along the radial direction, the moving direction of the gel precursor solution is from the smaller section side of the asymmetric channel to the larger section side of the asymmetric channel, the thickness of the gel precursor solution is gradually reduced in the moving process, and after the gel precursor solution is moved to a certain position, the gel precursor solution is solidified to form a gel membrane structure.
In the above process, a gel film structure of a target thickness can be obtained by controlling the moving position of the gel precursor solution. Compared with the prior art, the method provided by the invention can conveniently control the thickness of the gel film, and the gel film preparation method in the prior art is difficult to accurately control the thickness of the gel film.
In this example, the hydrogel material is driven by a pressure difference, in particular: applying pressure to the end of the asymmetric channel having the smaller cross-section using a gas or other phase that does not react with the gel precursor solution, thereby creating a pressure differential across the asymmetric channel.
In the embodiment, the section of the asymmetric channel comprises one of a circle, an ellipse and a polygon, and the radius of the section is 0.01-5 cm; the length of the asymmetric channel is 0.1-10 cm.
In this example, the volume of gel precursor solution required to prepare each gel film structure is 0.1-1000 uL; in preparing the gel film structure, the speed of the gel precursor solution moving in the asymmetric channel is controlled to be 0.01-2mm per minute.
In this embodiment, the gel membrane structure is bounded by the asymmetric channels at the periphery; the gel film structure has a thickness of 0.001-10 mm.
The invention also provides an embodiment of a method for preparing a gel film sensor, which comprises the following steps: the method comprises the following steps that an intelligent hydrogel material containing an intelligent recognition factor is adopted, the intelligent hydrogel material is driven to move in a hollow asymmetric channel through pressure difference, the area of the section of the asymmetric channel is gradually increased along the radial direction, the moving direction of the intelligent hydrogel material is from the smaller side of the section of the asymmetric channel to the larger side of the section of the asymmetric channel, the shape and thickness of the intelligent hydrogel material are gradually reduced in the moving process, and after the intelligent hydrogel material is moved to a certain position, the intelligent hydrogel material is solidified to form a gel membrane structure containing the intelligent recognition factor, namely the gel membrane sensor is obtained;
in this embodiment, the smart hydrogel material includes: gel precursor solution and intelligent recognition factors capable of recognizing the target to be detected; the intelligent recognition factor can generate detectable physical property transition under the target stimulation, including permeability and optical property transition.
In the method for preparing the gel film sensor of the present embodiment:
driving the intelligent hydrogel material by pressure difference, in particular: and applying pressure to the end with the smaller section of the asymmetric channel by using gas or other phases which do not react with the intelligent hydrogel material, so as to generate pressure difference on two sides of the asymmetric channel.
The section of the asymmetric channel comprises one of a circle, an ellipse and a polygon; the radius of the section is 0.01-5cm, and the length is 0.1-10 cm.
The volume of the intelligent hydrogel material required for preparing a gel film structure containing the intelligent hydrogel material is 0.1-1000 uL; in the preparation of the gel film structure, the speed of the intelligent hydrogel material moving in the asymmetric channel is controlled to be 0.01-2mm per minute.
Said gel membrane structure being bounded by said asymmetric channels at the periphery; the gel film structure has a thickness of 0.001-10 mm.
Specifically, in order to realize detection of copper ions in an environment, an embodiment of the present invention provides a method for preparing a corresponding gel film sensor, specifically including:
(1) preparing an intelligent hydrogel material for detecting copper ions:
gel precursor solution adopts gelma solution, and intelligent recognition factor adopts fluorescent molecular fluorescein sodium capable of recognizing copper ions; the photoinitiator adopts p-N, N-dimethylamino-benzoic acid isooctyl ester which can identify copper ions; wherein the gelma solution is methacrylated gelatin, the adopted gelma solution has the mass percentage concentration of 5-15 percent, the concentration of fluorescent molecular fluorescein sodium is 0.5-10mM, and the mass percentage concentration of p-N, N-dimethylaminobenzoic acid isooctyl ester is 0.1-1 percent;
the target copper ion will result in quenching of the fluorescein sodium fluorescence, with the fluorescence intensity being linearly inversely related to the copper ion concentration.
(2) Preparation of gel film: firstly, preparing an asymmetric channel, wherein the asymmetric channel is a tapered capillary channel in the embodiment, the cross section of the asymmetric channel is quadrilateral, the sectional area is gradually increased along the radial direction, 0.1-1uL of intelligent hydrogel material is sucked into the end with the smaller sectional area, a pressure generating device is connected into the end with the smaller sectional area, the pressure is increased to enable the intelligent hydrogel material to gradually move towards the end with the larger sectional area, the moving speed of the intelligent hydrogel material is 0.05-0.1mm/min, a liquid film with the thickness of about 0.2-1mm is formed after the intelligent hydrogel material moves to a certain position, and the liquid film is irradiated and cured by ultraviolet light to form a gel film structure;
(3) and (3) copper ion detection: and (3) contacting a sample to be detected with one side of the gel film structure to react, irradiating the sample based on exciting light after a certain time, recording the fluorescence intensity (shown in figure 3A), and analyzing to obtain detection data (figure 3B).
Specifically, in order to realize detection of copper ions in an environment, an embodiment of the present invention provides a method for preparing a corresponding gel film sensor, specifically including:
(1) preparing an intelligent hydrogel material for detecting potassium ions: the gel precursor solution adopts agarose solution, the intelligent recognition factor adopts nucleic acid aptamer capable of recognizing potassium ions, and the nucleic acid sequence: CGC GGG TTT TGG GTT TTG GGT TTT GGG G, respectively; and N-methyl porphyrin dipropionic acid capable of generating a fluorescence signal; wherein, the mass percentage concentration of the adopted agarose solution is 1-3 percent, the concentration of the aptamer is 0.5-2.5mM, and the concentration of the N-methylporphyrin dipropionic acid is 0.1-10 mM;
the N-methylporphyrin dipropionic acid can emit red fluorescence after the potassium ions are combined with the aptamer, and the fluorescence intensity is in positive correlation with the potassium ion concentration.
(2) Preparing a gel film sensor: as shown in fig. 1A, a pipetting gun is used to suck 2uL of gel precursor solution, then air is continuously sucked without changing the head of the pipetting gun, and the negative pressure generated by the pipetting gun pushes the gel precursor to move to a set position, at which time the gelation of the gel precursor is promoted, and a gel film structure is formed (as shown in fig. 2).
(3) And (5) detecting potassium ions. As shown in fig. 1B, a sample to be detected is contacted with one side of the gel film structure to react, excitation light is irradiated after a certain time, and a light intensity signal is read from the gel film to obtain a detection signal.
The present invention also provides an embodiment of a gel film sensor, prepared by the above method, a gel film sensor, prepared by the method, the gel film sensor comprising: the device comprises a hollow asymmetric channel and an intelligent gel film, wherein the periphery of the intelligent gel film is attached to the inner wall of the asymmetric channel; the intelligent gel film is formed after the intelligent hydrogel material is cured;
the cross-sectional area of the asymmetric channel gradually increases along the radial direction;
the intelligent gel film comprises an intelligent recognition factor capable of recognizing a target to be detected; the intelligent recognition factor is capable of undergoing a detectable transition in a physical property upon target stimulation.
Further, the cross section of the asymmetric channel comprises one of a circle, an ellipse, and a polygon; the radius range of the cross section is 0.01-5cm, and the length of the asymmetric channel is 0.1-10 cm;
the volume of the intelligent hydrogel material required for preparing each gel film sensor is 0.1-1000 uL; the thickness of the intelligent gel film in the gel film sensor is 0.001-10 mm.
Claims (10)
1. A method of making a gel film, comprising: the gel precursor solution is driven to move in the hollow asymmetric channel through pressure difference, the section area of the asymmetric channel is gradually increased along the radial direction, the moving direction of the gel precursor solution is from the smaller section side of the asymmetric channel to the larger section side of the asymmetric channel, the thickness of the gel precursor solution is gradually reduced in the moving process, and after the gel precursor solution is moved to a certain position, the gel precursor solution is solidified to form a gel membrane structure.
2. The method of claim 1, wherein the gel film structure of a target thickness is obtained by controlling the moving position of the gel precursor solution.
3. Method for the preparation of a gel film according to claim 1, characterized in that the gel precursor solution is driven by a pressure difference, in particular: applying pressure to the end of the asymmetric channel having the smaller cross-section using a gas or other phase that does not react with the gel precursor solution, thereby creating a pressure differential across the asymmetric channel.
4. The method of claim 1, wherein the cross section of the asymmetric channel comprises one of a circle, an ellipse and a polygon, and the radius of the cross section is in the range of 0.01-5 cm; the length of the asymmetric channel is 0.1-10 cm.
5. A method of producing a gel film according to claim 1, wherein the volume of the gel precursor solution required for producing each gel film structure is 0.1 to 1000 uL; in preparing the gel film structure, the speed of the gel precursor solution moving in the asymmetric channel is controlled to be 0.01-2mm per minute.
6. A method of preparing a gel film as claimed in claim 1 wherein said gel film structure is bounded by peripheral said asymmetric channels; the gel film structure has a thickness of 0.001-10 mm.
7. A method of making a gel film sensor, the method comprising: the method comprises the following steps that an intelligent hydrogel material containing an intelligent recognition factor is adopted, the intelligent hydrogel material is driven to move in a hollow asymmetric channel through pressure difference, the area of the section of the asymmetric channel is gradually increased along the radial direction, the moving direction of the intelligent hydrogel material is from the smaller side of the section of the asymmetric channel to the larger side of the section of the asymmetric channel, the shape and thickness of the intelligent hydrogel material are gradually reduced in the moving process, and after the intelligent hydrogel material is moved to a certain position, the intelligent hydrogel material is solidified to form a gel membrane structure containing the intelligent recognition factor, namely the gel membrane sensor is obtained;
the intelligent hydrogel material comprises: gel precursor solution and intelligent recognition factors capable of recognizing the target to be detected; the intelligent recognition factor is capable of undergoing a detectable transition in a physical property upon target stimulation.
8. The method for preparing a gel film sensor according to claim 7, wherein when the target to be detected is copper ions, in the intelligent hydrogel material, gelma solution is used as the gel precursor solution, and sodium fluorescein which can identify the copper ions is used as the intelligent identification factor; adding isooctyl p-dimethylaminobenzoate as a photoinitiator into the intelligent hydrogel material;
when the target to be detected is potassium ions, in the intelligent hydrogel material, the gel precursor solution adopts an agarose solution, the intelligent recognition factor adopts a nucleic acid aptamer capable of recognizing potassium ions, and the nucleic acid sequence is as follows: CGC GGG TTT TGG GTT TTG GGT TTT GGG G, respectively; and adding N-methyl porphyrin dipropionic acid capable of generating a fluorescence signal into the intelligent hydrogel material.
9. A gel film sensor prepared by the method of any one of claims 7-8, comprising: the device comprises a hollow asymmetric channel and an intelligent gel film, wherein the periphery of the intelligent gel film is attached to the inner wall of the asymmetric channel; the intelligent gel film is formed after the gel precursor solution is solidified;
the cross-sectional area of the asymmetric channel gradually increases along the radial direction;
the intelligent gel film comprises an intelligent recognition factor capable of recognizing a target to be detected; the intelligent recognition factor is capable of undergoing a detectable transition in a physical property upon target stimulation.
10. A gel film sensor according to claim 9, wherein the cross-section of the asymmetric channel comprises one of a circle, an ellipse, a polygon; the radius range of the cross section is 0.01-5cm, and the length of the asymmetric channel is 0.1-10 cm;
the volume of the intelligent hydrogel material required for preparing each gel film sensor is 0.1-1000 uL; the thickness of the intelligent gel film in the gel film sensor is 0.001-10 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110633530.XA CN113444272B (en) | 2021-06-07 | 2021-06-07 | Gel film and preparation method of gel film sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110633530.XA CN113444272B (en) | 2021-06-07 | 2021-06-07 | Gel film and preparation method of gel film sensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113444272A CN113444272A (en) | 2021-09-28 |
| CN113444272B true CN113444272B (en) | 2022-01-25 |
Family
ID=77810925
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110633530.XA Active CN113444272B (en) | 2021-06-07 | 2021-06-07 | Gel film and preparation method of gel film sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113444272B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110294858A (en) * | 2019-06-25 | 2019-10-01 | 常州大学 | It is a kind of can sequence detection anion, metal cation and dye molecule hydrogel sensing membrane and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8703904B2 (en) * | 2007-09-19 | 2014-04-22 | The Regents Of The University Of Colorado | Hydrogels and methods for producing and using the same |
| EP3291208B1 (en) * | 2016-08-31 | 2020-09-30 | Ricoh Company, Ltd. | Hydrogel structure, blood vessel, internal organ model, practice tool for medical procedure, and method of manufacturing the hydrogel structure |
-
2021
- 2021-06-07 CN CN202110633530.XA patent/CN113444272B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110294858A (en) * | 2019-06-25 | 2019-10-01 | 常州大学 | It is a kind of can sequence detection anion, metal cation and dye molecule hydrogel sensing membrane and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113444272A (en) | 2021-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ronkainen-Matsuno et al. | Electrochemical immunoassay moving into the fast lane | |
| Uchiyama et al. | Detection method for microchip separations | |
| JP6200419B2 (en) | Microfluidic disc for use in bead-based immunoassays | |
| Baker et al. | Recent advances in microfluidic detection systems | |
| JPS59147266A (en) | High sensitivity immunity analyzer using space pattern | |
| JP2003501639A (en) | Microfluidic devices for transverse and isoelectric focusing | |
| JPWO2004036194A1 (en) | Analysis chip and analyzer | |
| US20220226818A1 (en) | Detection chip and detection system | |
| Guihen | Recent advances in miniaturization—The role of microchip electrophoresis in clinical analysis | |
| KR20130045232A (en) | Methods for the detection of biologically relevant molecules and their interaction characteristics | |
| CN108593416A (en) | Micro-nano particle detection systems and method | |
| CN104492509A (en) | Micro-fluidic chip having nano dendrite Raman substrate and manufacturing method thereof | |
| US11905163B2 (en) | Micro-nano channel structure, sensor and manufacturing method thereof, and microfluidic device | |
| CN108593910A (en) | Based on microsphere supported particle detection systems and method | |
| CN104237183A (en) | Preparation method and application of ZnS quantum dot silica-based surface molecular imprinting sensor | |
| CN113444272B (en) | Gel film and preparation method of gel film sensor | |
| Tao et al. | Microfluidic devices with simplified signal readout | |
| CN113189181A (en) | Single-cell protein quantitative analysis method based on electrophoresis technology | |
| CN104646079A (en) | Centrifugal CD micro-fluidic chip for capillary electrophoresis and capillary gel electrophoresis device | |
| JP2000146910A (en) | Electrophoresis system | |
| Yang et al. | Highly accurate multiprotein detection on a digital ELISA platform | |
| WO2023066311A1 (en) | Single molecule/single cell detection chip | |
| JP2007292564A (en) | Biosensor chip | |
| CN111812091B (en) | Chip gel electrophoresis and online UV-VIS imaging detection device thereof | |
| Yang et al. | A scanning laser induced fluorescence detection system for capillary electrophoresis microchip based on optical fiber |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |