CN113418913A - 一种稳定亚硝酸盐试纸及其制备方法 - Google Patents
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Abstract
本发明公开了一种稳定亚硝酸盐试纸的制备方法,其特征在于,该方法包括以下步骤:浸泡液的配制:将酒石酸、磺胺以及盐酸萘乙二胺溶于水中配制成浸泡液;试纸的制备:将快速定量滤纸浸入上述浸泡液中,浸泡时间2~5分钟,取出后干燥,然后浸入保护剂涂饰液中5~15秒,取出立即烘干,制成的试纸呈白色;测试条制备:将上述试纸裁成6mm的纸条,用胶水或双面胶粘贴在塑料板上,用切纸机裁成0.6mm的试纸条,避光保存。本发明的亚硝酸盐试纸对亚硝酸盐检测范围为0~80mg/L,适用于地表水、污水、海水等水体中亚硝酸盐检测。本发明的试纸稳定性好,常温下能够温度保存。
Description
技术领域
本发明属于环境检测技术领域,具体涉及一种稳定亚硝酸盐试纸及其制备方法。
背景技术
亚硝酸盐在土壤、天然水源、食品和生理***中广泛存在。亚硝酸盐会通过对鱼类和甲壳类等水生动物的毒性而影响水产养殖业,因亚硝酸盐在高密度养殖***中可达到毒性浓度而对高浓度生物种群致命。高浓度的亚硝酸盐会对生态生理***带来富营养化和隐患,对人体和其他生物体的健康有害。
随着人口增长、工业化和城镇化进程的迅速推进,水资源短缺和水污染日益严重。生活污水、工业废水和径流污水等人类生产、生活活动中产生了大量污水。亚硝酸盐是水中重要的含氮污染物,水中污染物技术指标的检测,使用专业的检测分析仪器,采用国家标准方法可以得到准确的检测结果,但是存在以下不足,需要特定的设备、操作繁琐、专业要求高、应急困难等。为了弥补上述检测的短板,近年来开发了快速、准确、携带方便、操作简单、快捷的亚硝酸盐检测试纸。
现有的亚硝酸盐试纸都是采用格里斯试剂比色法的原理。由于负载在滤纸上的格里斯试剂极易吸湿、遇光易变色失效等缺点,试纸的需要在2~10℃条件保存,造成使用不便。
发明内容
本发明的主要目的在针于对现有技术中的缺陷,提供一种稳定亚硝酸盐试纸及其制备方法。本发明的亚硝酸盐试纸稳定性好,能够常温储存。
本发明的目的及解决其技术问题是采用以下技术方案来实现的。
本发明提供了一种稳定亚硝酸盐试纸的制备方法,该方法包括以下步骤:
浸泡液的配制:将酒石酸、磺胺以及盐酸萘乙二胺溶于水中配制成浸泡液;
试纸的制备:将快速定量滤纸浸入上述浸泡液中,浸泡时间2~5分钟,取出后干燥,然后浸入保护剂涂饰液中5~15秒,取出立即烘干,制成的试纸呈白色;
测试条制备:将上述试纸裁成6mm的纸条,用胶水或双面胶粘贴在塑料板上,用切纸机裁成0.6mm的试纸条,避光保存。
优选地,按照重量百分比计,所述浸泡液中各成分含量为:酒石酸2~20%、磺胺0.2~1.5%、盐酸萘乙二胺0.03~0.3%,其余为水。
优选地,所述干燥条件为:温度40~60℃,时间2~3h。
优选地,所述保护剂涂饰液选自以下组中任意一种或两种以上的混合物的水溶液,该组为:二氧化硅、改性淀粉、羧甲基纤维素钠、黄原胶、海藻酸钠。
优选地,所述保护剂涂饰液浓度为0.1~0.5%。
优选地,所述烘干条件为:温度40~60℃,时间2~3h。
优选地,所述塑料板的厚度为0.1~0.3mm、宽度为5~8mm。
本发明的目的及解决其技术问题还通过采用以下技术方案来实现。
本发明还提供了一种稳定亚硝酸盐试纸,所述稳定亚硝酸盐试纸为根据权利要求1~6中任一项所述的方法制备得到,所述试纸包括基片和显色剂纸片。
优选地,所述试纸的检测范围为:亚硝酸盐0~80mg/L。
借由上述技术方案,本发明至少具有下列优点:本发明提供了一种稳定亚硝酸盐试纸及其制备方法,本发明的制备方法步骤简单,所得到的亚硝酸盐试纸稳定性好,能够室温贮存12个月,使用方便、快捷、测试数据准确可靠。本发明的亚硝酸盐试纸对亚硝酸盐检测范围为0~80mg/L,适用于地表水、污水、海水等水体中亚硝酸盐检测。本发明的试纸还配有标准比色卡,检测速度快,准确率高。
上述说明仅是本发明技术方案的概述,为了能够更清楚了解本发明的技术手段,并可依照说明书的内容予以实施,以下以本发明的较佳实施例详细说明如后。
具体实施方式
为了使本发明实现的技术手段、创作特征、达成目的与功效易于明白了解,下面将结合本发明实施例,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其它实施例,都属于本发明保护的范围。
实施例1 亚硝酸盐试纸的制备
浸泡液配制:称取酒石酸2g、磺胺0.2g、盐酸萘乙二胺0.05g,加入100mL水溶解。
保护剂涂饰液:称取0.2g海藻酸钠,加入100mL水溶解。
试纸的制备:将300*200mm快速定量滤纸浸入上述浸泡液中,浸泡时间2分钟,取出放入50℃干燥箱干燥2小时,浸入保护剂涂饰液中5~15秒,取出立即烘干。制成的试纸呈白色。
测试条制备:将上述试纸裁成6mm的纸条,用胶水或双面胶粘贴在厚度0.15mm、宽度6mm的塑料板上,用切纸机裁成0.6mm的试纸条。装入有干燥剂、避光的试纸桶中保存。
实施例2 标准比色卡的制备
用亚硝酸钠配制系列亚硝酸盐标准溶液:亚硝酸盐浓度分别为:0mg/L、2.0mg/L、5.0mg/L、10mg/L、20mg/L、40mg/L、80mg/L,用实施例1制备的试纸分别进行测试。
测试方法:将试纸浸入水样1秒,甩掉多余水分,静置30秒后与国际潘通色卡进行对比,记录色号,印刷。
试验例1 试纸稳定性测试
将按照实施例1制备的硝酸盐试纸,装入有干燥剂、避光的试纸桶中室温保存12个月,进行测试。
系列亚硝酸盐标准溶液,亚硝酸盐浓度分别为:0mg/L、2.0mg/L、5.0mg/L、10mg/L、20mg/L、40mg/L、80mg/L。
测试方法:将试纸浸入标准溶液中1秒,甩掉多余水分,静置30秒后和标准色卡比较,读取数值,其测试结果与相应浓度的浓度一致。
试验例2 试纸稳定性测试
将按照实施例1制备的硝酸盐试纸,装入有干燥剂、避光的试纸桶中放入38℃培养箱中,保存28天,进行测试。
系列亚硝酸盐标准溶液,亚硝酸盐浓度分别为:0mg/L、2.0mg/L、5.0mg/L、10mg/L、20mg/L、40mg/L、80mg/L。
测试方法:将试纸浸入标准溶液中1秒,甩掉多余水分,静置30秒后和标准色卡比较,读取数值,其测试结果与相应浓度的浓度一致。
试验例3 试纸测试实验
测试水样为污水处理厂二沉池出水,用实施例1制备的试纸,将试纸浸入水样1秒,甩掉多余水分,静置30秒后和标准色卡比较,读取数值。测试结果亚硝酸盐浓度超过80mg/L时,水样稀释后重新测试。
试纸测试结果与国标法测试结果对比见表1。
表1 测试结果
由表1的结果可以看出,本发明的试纸检测结果与国标法的结果接近,准确度高,适于广泛使用。
以上所述,仅是本发明的较佳实施例而已,并非对本发明作任何形式上的限制,虽然本发明已以较佳实施例揭露如上,然而并非用以限定本发明,任何熟悉本专业的技术人员,在不脱离本发明技术方案范围内,当可利用上述揭示的方法及技术内容作出些许的更动或修饰为等同变化的等效实施例,但凡是未脱离本发明技术方案的内容,依据本发明的技术实质对以上实施例所作的任何简单修改、等同变化与修饰,均仍属于本发明技术方案的范围内。
Claims (9)
1.一种稳定亚硝酸盐试纸的制备方法,其特征在于,该方法包括以下步骤:
浸泡液的配制:将酒石酸、磺胺以及盐酸萘乙二胺溶于水中配制成浸泡液;
试纸的制备:将快速定量滤纸浸入上述浸泡液中,浸泡时间2~5分钟,取出后干燥,然后浸入保护剂涂饰液中5~15秒,取出立即烘干,制成的试纸呈白色;
测试条制备:将上述试纸裁成6mm的纸条,用胶水或双面胶粘贴在塑料板上,用切纸机裁成0.6mm的试纸条,避光保存。
2.根据权利要求1所述的制备方法,其特征在于,按照重量百分比计,所述浸泡液中各成分含量为:酒石酸2~20%、磺胺0.2~1.5%、盐酸萘乙二胺0.03~0.3%,其余为水。
3.根据权利要求1所述的制备方法,其特征在于,所述干燥条件为:温度40~60℃,时间2~3h。
4.根据权利要求1所述的制备方法,其特征在于,所述保护剂涂饰液选自以下组中任意一种或两种以上的混合物的水溶液,该组为:二氧化硅、改性淀粉、羧甲基纤维素钠、黄原胶、海藻酸钠。
5.根据权利要求1所述的制备方法,其特征在于,所述保护剂涂饰液浓度为0.1~0.5%。
6.根据权利要求1所述的制备方法,其特征在于,所述烘干条件为:温度40~60℃,时间2~3h。
7.根据权利要求1所述的制备方法,其特征在于,所述塑料板的厚度为0.1~0.3mm、宽度为5~8mm。
8.一种稳定亚硝酸盐试纸,其特征在于,所述稳定亚硝酸盐试纸为根据权利要求1~7中任一项所述的方法制备得到,所述试纸包括基片和显色剂纸片。
9.根据权利要求8所述的稳定亚硝酸盐试纸,其特征在于,所述试纸的检测范围为:亚硝酸盐0~80mg/L。
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