CN113402037A - Application of Xanthocarpus in treatment of heavy metal polluted wastewater - Google Patents
Application of Xanthocarpus in treatment of heavy metal polluted wastewater Download PDFInfo
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- CN113402037A CN113402037A CN202110789828.XA CN202110789828A CN113402037A CN 113402037 A CN113402037 A CN 113402037A CN 202110789828 A CN202110789828 A CN 202110789828A CN 113402037 A CN113402037 A CN 113402037A
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- copper
- kocuria
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- 239000002351 wastewater Substances 0.000 title claims abstract description 33
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 13
- 241000460492 Kocuria flava Species 0.000 claims abstract description 31
- 239000010949 copper Substances 0.000 claims abstract description 30
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical group [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229910052802 copper Inorganic materials 0.000 claims abstract description 20
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229910001431 copper ion Inorganic materials 0.000 claims abstract description 11
- 239000002609 medium Substances 0.000 claims description 23
- 239000001963 growth medium Substances 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 8
- 239000004202 carbamide Substances 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 8
- 241000050051 Chelone glabra Species 0.000 abstract description 4
- 238000004065 wastewater treatment Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 12
- 241000579722 Kocuria Species 0.000 description 9
- 241000710831 Flavivirus Species 0.000 description 8
- 150000002500 ions Chemical class 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 5
- 108010046334 Urease Proteins 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- 241000589636 Xanthomonas campestris Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 241000710781 Flaviviridae Species 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 1
- 229910021532 Calcite Inorganic materials 0.000 description 1
- 241000222511 Coprinus Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 101000965313 Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) Aconitate hydratase A Proteins 0.000 description 1
- 241000192041 Micrococcus Species 0.000 description 1
- 238000003723 Smelting Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 238000009388 chemical precipitation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 239000012776 electronic material Substances 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Removal Of Specific Substances (AREA)
Abstract
The invention relates to the technical field of heavy metal polluted wastewater treatment, in particular to application of Xanthocarpus in treating heavy metal polluted wastewater. The heavy metal polluted wastewater is preferably copper polluted wastewater, and the concentration of copper ions in the copper polluted wastewater is 100-1000 mg/L. The invention discovers that the yellow-flowered skullcap (Kocuria flavus) has obvious effect in treating the copper-polluted wastewater, and the yellow-flowered skullcap (Kocuria flavus) can effectively remove more than 90% of copper ions in the copper-polluted wastewater; the strain is very environment-friendly, has strong adaptability, low propagation cost and great application potential.
Description
Technical Field
The invention relates to the technical field of heavy metal polluted wastewater treatment, in particular to application of Xanthocarpus in treating heavy metal polluted wastewater.
Background
Waste water containing a large amount of copper ions is often generated in the processes of chemical industry, printing and dyeing, electroplating, nonferrous smelting, mining of nonferrous metals, rinsing waste water of electronic materials, dye production and the like. The main treatment methods of the copper-containing wastewater include a chemical precipitation method, an ion exchange method, an electrolysis method and the like. However, these methods use energy and chemicals at high cost, and thus it is necessary to find a low-cost and environmentally friendly treatment method.
Microbial remediation is receiving increasing attention as an effective biotechnology for remediating contaminated environments. Microbial Induced Calcification (MICP) offers another option to solve this problem. Urea-decomposing bacteria in the environment are capable of decomposing urea and secreting one or more metabolites that react with ions and compounds in the environment and produce mineral particle deposits.
In view of this, the invention is particularly proposed.
Disclosure of Invention
In order to promote the biological treatment research of the heavy metal polluted wastewater (especially the copper polluted wastewater), the invention provides the application of the Kocuria flavus (Kocuria flavus) in the treatment of the heavy metal polluted wastewater.
Specifically, the invention provides the following technical scheme:
the invention provides an application of yellow curia flavus (Kocuria flavus) in treating heavy metal polluted wastewater.
Preferably, the heavy metal polluted wastewater is copper polluted wastewater.
In the present invention, the Kocuria flavus (Kocuria flavus) is particularly suitable for treating copper-contaminated wastewater.
Preferably, the concentration of copper ions in the copper polluted wastewater is 100-1000 mg/L.
Further, the concentration of copper ions in the copper polluted wastewater is 100mg/L, 250mg/L, 500mg/L, 750mg/L or 1000 mg/L.
Preferably, the application comprises: adding fermentation liquor of yellow curia flavus into the copper polluted wastewater to be treated;
wherein the culture medium of the fermentation liquor is NB cultureMedium or NBU medium; the NBU culture medium contains 2% urea and 25mM CaCl2NB medium of (1).
Further, the culture medium of the fermentation broth is NBU culture medium.
Preferably, the formula of the NB medium is as follows: 10g/L of peptone, 3g/L of beef extract powder, 5g/L of sodium chloride and 8.0 of pH.
The invention has the beneficial effects that:
the invention discovers that the yellow-flowered skullcap (Kocuria flavus) has obvious effect in treating the copper-polluted wastewater, and the yellow-flowered skullcap (Kocuria flavus) can effectively remove more than 90% of copper ions in the copper-polluted wastewater; the strain is very environment-friendly, has strong adaptability, low propagation cost and great application potential.
Drawings
FIG. 1 is a graph showing the copper removal rate and urease activity of Kocuria flavus (Kocuria flavus) as a function of time;
FIG. 2 is a graph showing the growth of Xanthomonas campestris (Kocuria flavus) and the Cu removal rate as a function of Cu concentration.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or instruments used are conventional products available from regular distributors, not indicated by the manufacturer.
The formulation of NB medium used in the following examples is as follows: 10g/L of peptone, 3g/L of beef extract powder, 5g/L of sodium chloride and 8.0 of pH.
The formulation of the urea agar medium used in the following examples is as follows: 1.0g/L of gastrone, 1.0g/L of D-glucose, 5.0g/L of sodium chloride, 1.2g/L of disodium hydrogen phosphate, 0.8g/L of dipotassium hydrogen phosphate, 0.012g/L of red and 15.0g/L of agar; the preparation method comprises the following steps:
1) weighing 24g of urea agar base, and resuspending with 950ml of deionized water;
2) heating and boiling until the mixture is completely dissolved;
3) sterilizing at 115 deg.C for 20 min;
4) cooling to 50 deg.C, adding 50ml sterile 40% urea solution;
5) mixing, packaging 10ml into sterile test tube, and preparing agar slant.
NBU medium used in the following examples was 2% urea (filter sterilized) and 25mM CaCl2NB medium of (1).
Example 1 isolation and screening of Xanthomonas campestris (Kocuria flavus)
1) Collecting a copper-polluted soil sample by using a screw cap disinfection bottle;
2) 1g of a copper-contaminated soil sample was inoculated into 50ml of a solution containing 100mg/L copper (CuSO)4·5H2O) in nutrient broth (NB medium) for 48 hours at 30 ℃ under shaking conditions (130 rpm);
3) bacteria were counted using serial dilution full plate counting method using nutrient agar with different copper concentrations (25, 50 and 100 mg/L);
4) the petri dish was incubated overnight at 30 ℃; subsequently, the colonies were transferred onto urea agar medium (urease selection medium) to check urease production (since urease is a key indicator of calcite production);
5) yellow kukoku fungus (Kocuria flavus) was finally selected in view of higher urease-producing ability and growth ability in a copper-containing medium; the strain and the extracts of the fermentation supernatant and the thallus thereof have obvious effect on treating the copper-polluted wastewater.
Example 2 identification of Xanthomonas campestris (Kocuria flavus)
1) The strain obtained by screening in the example 1 is cultured overnight, and the genome DNA is extracted by an alkaline lysis method;
2) amplifying the 16S rRNA gene in the genome DNA by PCR;
3) the 16S rRNA amplicon was gel eluted and ligated into pTZ57R/T vector (enzyme company, USA) according to the manufacturer' S instructions;
4) sequencing by using an automatic sequencer of an applied biological system; kuelia flava (Kocuria flavus) is gram-positive and belongs to the phylum Actinomyceta and Micrococcus;
5) phylogenetic analysis of 16S rDNA revealed that the gene of Kocuria flaviviruses (Kocuria flaviviruses) had 100% similarity to the commercial Kocuria flaviviruses (Kocuria flaviviruses).
Example 3 use of Coprinus flavus (Kocuria flavus) for treating copper-contaminated wastewater
1) To a 100ml flask, 50ml of NB medium containing 100mg/L of Cu ions and 50ml of NBU medium containing 100mg/L of Cu ions were added, respectively;
2) inoculating yellow curia flaviviridae (Kocuria flaviviridae) to the two culture mediums in step 1), as shown in fig. 1, wherein 95% of copper ions (100mg/L) are effectively removed in NBU culture medium within 120 hours; in NB medium, 68% of copper ions were effectively removed.
In this example, the selected Kocuria flaviviruses (Kocuria flavus) can be isolated according to the method of example 1, or commercially available Kocuria flaviviruses (Kocuria flavus) can be selected; that is, the isolated yellow Kurilla (Kocuria flavus) and the commercially available yellow Kurilla (Kocuria flavus) in example 1 can achieve the technical effects of the present invention.
Example 4 application of Kukukola (Kocuria flavus) to treatment of copper-contaminated wastewater
1) The effect on the growth of Kocuria flavus and the copper removal effect were examined using copper ion solutions of different concentrations (250mg/L, 500mg/L, 750mg/L and 1000 mg/L);
the method specifically comprises the following steps: 50ml of NBU medium containing 250mg/L of Cu ions, 50ml of NBU medium containing 500mg/L of Cu ions, 50ml of NBU medium containing 750mg/L of Cu ions, and 50ml of NBU medium containing 1000mg/L of Cu ions were added to 100ml of flasks, and then Cocuria flavus (Kocuria flavus) was inoculated to each medium;
2) as shown in fig. 2, the yellow curia (Kocuria flavus) effectively removed a higher amount of Cu at a high concentration of Cu; in the NBU culture medium containing 250mg/L, the removal rate of copper is 92 percent; in the NBU culture medium containing 500mg/L, the removal rate of copper is 95 percent; in NBU culture medium containing 750mg/L, the removal rate of copper is 96%; the removal rate of copper in NBU medium containing 1000mg/L was 97%.
In this example, the selected Kocuria flaviviruses (Kocuria flavus) can be isolated according to the method of example 1, or commercially available Kocuria flaviviruses (Kocuria flavus) can be selected; that is, the isolated yellow Kurilla (Kocuria flavus) and the commercially available yellow Kurilla (Kocuria flavus) in example 1 can achieve the technical effects of the present invention.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. Application of Kocuria flavus (Kocuria flavus) in treating heavy metal polluted wastewater.
2. Use according to claim 1, wherein the heavy metal contaminated wastewater is copper contaminated wastewater.
3. The application of claim 2, wherein the concentration of copper ions in the copper polluted wastewater is 100-1000 mg/L.
4. The use according to claim 3, wherein the copper ion concentration in the copper-contaminated wastewater is 100mg/L, or 250mg/L, or 500mg/L, or 750mg/L, or 1000 mg/L.
5. Use according to any one of claims 1 to 4, comprising: adding fermentation liquor of yellow curia flavus into the copper polluted wastewater to be treated;
wherein the culture medium of the fermentation liquor is NB culture medium or NBU culture medium; the NBU culture medium contains 2% urea and 25mM CaCl2NB medium of (1).
6. The use according to claim 5, wherein the culture medium of the fermentation broth is NBU medium.
7. The use according to claim 5, wherein the formulation of NB medium is as follows: 10g/L of peptone, 3g/L of beef extract powder, 5g/L of sodium chloride and 8.0 of pH.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110789828.XA CN113402037A (en) | 2021-07-13 | 2021-07-13 | Application of Xanthocarpus in treatment of heavy metal polluted wastewater |
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| CN202110789828.XA CN113402037A (en) | 2021-07-13 | 2021-07-13 | Application of Xanthocarpus in treatment of heavy metal polluted wastewater |
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Citations (8)
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|---|---|---|---|---|
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| CN104673709A (en) * | 2014-12-26 | 2015-06-03 | 环境保护部华南环境科学研究所 | Bacterial strain YB-03 for adsorbing heavy metals and enhancing phytoextraction of heavy metals and application thereof |
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-
2021
- 2021-07-13 CN CN202110789828.XA patent/CN113402037A/en active Pending
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Application publication date: 20210917 |