CN113396935A - Streptomyces griseofuscus bactericide and preparation method thereof - Google Patents
Streptomyces griseofuscus bactericide and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
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- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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Abstract
The invention discloses a streptomyces griseofuscus bactericide and a preparation method thereof, relates to the related field of bactericides, and aims to solve the problem that the streptomyces in the prior art can also influence the growth of grains or plants because the streptomyces rapidly grows and breeds to deprive nutrients and moisture, occupy space and consume oxygen. The method comprises the following steps: fermentation products of streptomyces griseofuscus, swelling soil, active enzyme, dispersing agent, glucose, Chinese medicine residue humus filtrate, vinegar and clear water; the method comprises the following steps: adding active enzyme into the fermentation product of the streptomyces griseofuscus, and adding expansive soil to obtain a product A; step two: mixing the Chinese medicine residue humus filtrate with glucose, and adding expansive soil to obtain a product B; step three: mixing the product A and the product B, adding clear water and a dispersing agent, mixing, and then adding table vinegar; step four: continuously adding expansive soil, drying and crushing; step five: adding clear water, filtering to obtain filtrate and powder, and pressing the powder into granules to obtain granular bactericide and liquid bactericide.
Description
Technical Field
The invention relates to the related field of bactericides, and particularly relates to a streptomyces griseofuscus bactericide and a preparation method thereof.
Background
Due to the rapid growth of population and the fact that the production yield of grains cannot reach the grain consumption, methods for improving the grain yield are researched all over the world, and meanwhile, various chemically synthesized pesticides are used for reducing the conditions of germs and insect pests in the grain growth process. However, the wide application of chemical pesticides causes problems of environmental pollution, pesticide residue, ecological balance damage, drug resistance of germs and the like, so biological pesticides are developed in various countries to replace chemical synthetic pesticides, thereby improving the grain yield.
Streptomyces is a biocontrol bacterium, and can be used for preventing and treating various plant diseases. Streptomyces can deprive nutrients and water, occupy space, consume oxygen, etc. by growing and multiplying rapidly, so as to weaken and eliminate certain pathogens in the same living environment.
But the streptomycete can also affect the growth of grains or plants because the streptomycete takes nutrients and water, occupies space and consumes oxygen due to the rapid growth and reproduction of the streptomycete; therefore, the market urgently needs to develop a streptomyces griseofuscus bactericide and a preparation method thereof to help people solve the existing problems.
Disclosure of Invention
The invention aims to provide a streptomyces griseofuscus bactericide and a preparation method thereof, and aims to solve the problem that the streptomyces proposed in the background art can also influence the growth of grains or plants because the streptomyces rapidly grows and breeds to deprive nutrients and water, occupy space and consume oxygen.
In order to achieve the purpose, the invention provides the following technical scheme: a Streptomyces griseofuscus bactericide, comprising: fermentation products of streptomyces griseofuscus, bentonite, active enzyme, a dispersing agent, glucose, Chinese medicine residue humus filtrate, vinegar and clear water.
Preferably, the streptomyces griseofuscus is preserved in China general microbiological culture Collection center (CGMCC), and the preservation number is CGMCC NO. 16141.
Preferably, the streptomyces griseofuscus bactericide comprises the following components in percentage by mass: 30-45 parts of fermentation product of streptomyces griseofuscus, swelled ground, 10-18 parts of active enzyme, 20-25 parts of dispersing agent, 10-16 parts of glucose, 5-9 parts of Chinese medicine residue humus filtrate, 5-9 parts of vinegar and the balance of clear water.
Preferably, the streptomyces griseofuscus bactericide comprises the following components in percentage by mass: 40 parts of fermentation product of streptomyces griseofuscus, bentonite, 12 parts of active enzyme, 20 parts of dispersing agent, 10 parts of glucose, 6 parts of Chinese medicine residue humus filtrate, 6 parts of vinegar and the balance of clear water.
Preferably, the streptomyces griseofuscus bactericide comprises the following components in percentage by mass: 40 parts of fermentation product of streptomyces griseofuscus, bentonite, 12 parts of active enzyme, 25 parts of dispersing agent, 15 parts of glucose, 9 parts of Chinese medicine residue humus filtrate, 9 parts of vinegar and the balance of clear water.
Preferably, the preparation process of the Chinese medicine residue humus filtrate is as follows: filling the Chinese medicine residue into a container, adding clear water and cleaned large-particle stones, stirring to disperse the stones into the Chinese medicine residue, waiting, and filtering to obtain Chinese medicine residue humus filtrate after the Chinese medicine residue is rotten.
Preferably, the streptomyces griseofuscus is inoculated in a culture medium, the pH value is 7.0, the fermentation temperature is 36-39 ℃, the rotation speed is 180-.
Preferably, the inoculation amount of the activated strain in the culture process of the streptomyces griseofuscus strain is 6%.
A preparation method comprises the following steps:
the method comprises the following steps: adding active enzyme into the fermentation product of the streptomyces griseofuscus, uniformly mixing, and adding expansive soil to obtain a product A;
step two: uniformly mixing the Chinese medicine residue humus filtrate and glucose, and adding expansive soil to obtain a product B;
step three: mixing the product A and the product B, adding a proper amount of clear water and a dispersing agent, mixing again, and adding table vinegar after mixing uniformly;
step four: continuously adding expansive soil into the mixed solution, and drying and crushing the product;
step five: adding clear water into the crushed material, stirring uniformly, filtering to obtain filtrate and powder, and pressing the powder into granules to obtain granular bactericide and liquid bactericide.
Compared with the prior art, the invention has the beneficial effects that:
1. in the invention, glucose, Chinese medicine residue humus filtrate and vinegar are added; glucose is an organic substance, and is converted into humic acid under the action of microorganisms generated by fermentation products of streptomyces griseofuscus after being applied to soil, so that the soil fertility is improved, the growth of crops is promoted, the stress resistance of plants is increased, the growth and the propagation of the plants are promoted, the plants are strong, and the fruit yield is improved; the Chinese medicine residue humus contains rich organic matters and inorganic substances, and nitrogen, phosphorus and potassium fertilizers required by plant growth can supplement nutrient substances required by the plant growth process in time; the vinegar can promote absorption of microelements such as phosphorus and iron, and keep branches and leaves in evergreen state. By supplementing nutrient substances, the yield of the plants can be improved, and the influence of microorganisms on the growth of the plants due to normal robbing of part of nutrients can be reduced.
2. In the invention, the streptomyces griseofuscus has a high effect of inhibiting the activity of bacteria, the strain inoculation amount is 6%, the pH is 7.0, the fermentation temperature is 36-39 ℃, the rotation speed is 180-200rpm, the fermentation culture time is 5-6d, and the activity of the fermentation product of the streptomyces griseofuscus obtained by filtering is stronger, so that the growth of the bacteria can be inhibited more obviously, and the sterilization effect is achieved.
3. Firstly, adding active enzyme into a fermentation product of streptomyces griseofuscus, uniformly mixing, and adding expansive soil to obtain a product A; uniformly mixing the Chinese medicine residue humus filtrate and glucose, and adding expansive soil to obtain a product B; mixing the product A and the product B, adding a proper amount of clear water and a dispersing agent, mixing again, and adding table vinegar after mixing uniformly; add bentonite to the mixed solution and dry and pulverize the product. Adding clear water into the crushed materials, stirring uniformly, filtering to obtain filtrate and powder, and pressing the powder into granules to obtain granular bactericide and liquid bactericide. When the bactericide is used, the granular bactericide can be buried in soil as a slow release fertilizer, so that microorganisms of the bactericide slowly release the microorganisms, the bactericide is accurately sterilized, and the effect duration of the bactericide is ensured; the liquid bactericide is directly sprayed on plants, and has the function of quickly sterilizing and releasing nutrient substances. Through the matching of the granular bactericide and the liquid bactericide, the bactericide can sterilize quickly and for a long time.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
Example 1
The embodiment provided by the invention comprises the following steps: the streptomyces griseofuscus bactericide comprises the following components in parts by mass: 30-45 parts of fermentation product of streptomyces griseofuscus, swelled ground, 10-18 parts of active enzyme, 20-25 parts of dispersing agent, 10-16 parts of glucose, 5-9 parts of Chinese medicine residue humus filtrate, 5-9 parts of vinegar and the balance of clear water.
Glucose is an organic substance, and is converted into humic acid under the action of microorganisms generated by fermentation products of streptomyces griseofuscus after being applied to soil, so that the soil fertility is improved, the growth of crops is promoted, the stress resistance of plants is increased, the growth and the propagation of the plants are promoted, the plants are strong, and the fruit yield is improved;
the Chinese medicine residue humus contains rich organic matters and inorganic substances, and nitrogen, phosphorus and potassium fertilizers required by plant growth can supplement nutrient substances required by the plant growth process in time;
the vinegar can promote absorption of microelements such as phosphorus and iron, and keep branches and leaves in evergreen state.
Further, the preparation process of the Chinese medicine residue humus filtrate is as follows: filling the Chinese medicine residue into a container, adding clear water and cleaned large-particle stones, stirring to disperse the stones into the Chinese medicine residue, waiting, and filtering to obtain Chinese medicine residue humus filtrate after the Chinese medicine residue is rotten.
Further, inoculating the streptomyces griseofuscus into a culture medium, wherein the inoculation amount of an activated strain in the culture process of the streptomyces griseofuscus strain is 6%, the pH value is 7.0, the fermentation temperature is 36-39 ℃, the rotation speed is 180-200rpm, the fermentation culture time is 5-6d, and filtering is carried out to obtain a fermentation product of the streptomyces griseofuscus.
Example 2
The embodiment provided by the invention comprises the following steps: the streptomyces griseofuscus bactericide comprises the following components in percentage by mass: 40 parts of fermentation product of streptomyces griseofuscus, bentonite, 12 parts of active enzyme, 20 parts of dispersing agent, 10 parts of glucose, 6 parts of Chinese medicine residue humus filtrate, 6 parts of vinegar and the balance of clear water.
Further, the preparation process of the Chinese medicine residue humus filtrate is as follows: filling the Chinese medicine residue into a container, adding clear water and cleaned large-particle stones, stirring to disperse the stones into the Chinese medicine residue, waiting, and filtering to obtain Chinese medicine residue humus filtrate after the Chinese medicine residue is rotten.
Further, the streptomyces griseofuscus is inoculated on a culture medium, the inoculation amount of an activated strain in the culture process of the streptomyces griseofuscus strain is 6%, the pH value is 7.0, the fermentation temperature is 39 ℃, the rotating speed is 200rpm, the fermentation culture time is 6 days, and a fermentation product of the streptomyces griseofuscus is obtained by filtering.
Example 3
The embodiment provided by the invention comprises the following steps: the streptomyces griseofuscus bactericide comprises the following components in percentage by mass: 40 parts of fermentation product of streptomyces griseofuscus, bentonite, 12 parts of active enzyme, 25 parts of dispersing agent, 15 parts of glucose, 9 parts of Chinese medicine residue humus filtrate, 9 parts of vinegar and the balance of clear water.
Further, the preparation process of the Chinese medicine residue humus filtrate is as follows: filling the Chinese medicine residue into a container, adding clear water and cleaned large-particle stones, stirring to disperse the stones into the Chinese medicine residue, waiting, and filtering to obtain Chinese medicine residue humus filtrate after the Chinese medicine residue is rotten.
Further, the streptomyces griseofuscus is inoculated on a culture medium, the inoculation amount of an activated strain in the culture process of the streptomyces griseofuscus strain is 6%, the pH value is 7.0, the fermentation temperature is 36 ℃, the rotating speed is 180rpm, the fermentation culture time is 5 days, and a fermentation product of the streptomyces griseofuscus is obtained by filtering.
The obtained fermentation product of the streptomyces griseofuscus has stronger activity, and after the bactericide is prepared subsequently, microorganisms in the bactericide have stronger effect on inhibiting the activity of bacteria.
Example 4
The embodiment provided by the invention comprises the following steps: a preparation method comprises the following steps:
the method comprises the following steps: adding active enzyme into the fermentation product of the streptomyces griseofuscus, uniformly mixing, and adding expansive soil to obtain a product A;
step two: uniformly mixing the Chinese medicine residue humus filtrate and glucose, and adding expansive soil to obtain a product B;
step three: mixing the product A and the product B, adding a proper amount of clear water and a dispersing agent, mixing again, and adding table vinegar after mixing uniformly;
step four: continuously adding expansive soil into the mixed solution, and drying and crushing the product; the drying temperature is kept at a lower level, so that the influence on the activity of microorganisms is avoided;
step five: adding clear water into the crushed material, stirring uniformly, filtering to obtain filtrate and powder, and pressing the powder into granules to obtain granular bactericide and liquid bactericide.
The granular bactericide is buried in soil as a slow release fertilizer, so that microorganisms of the bactericide slowly release the microorganisms, the bactericide is accurately sterilized, and the effect duration of the bactericide is ensured; the liquid bactericide is directly sprayed on plants, and has the function of quickly sterilizing and releasing nutrient substances. Through the matching of the granular bactericide and the liquid bactericide, the bactericide can sterilize quickly and for a long time.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (9)
1. The streptomyces griseofuscus bactericide is characterized by comprising the following components: fermentation products of streptomyces griseofuscus, bentonite, active enzyme, a dispersing agent, glucose, Chinese medicine residue humus filtrate, vinegar and clear water.
2. The streptomyces griseofuscus fungicide according to claim 1, characterized in that: the streptomyces griseofuscus is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 16141.
3. The streptomyces griseofuscus bactericide as claimed in claim 1, wherein the streptomyces griseofuscus bactericide comprises the following components in mass fraction: 30-45 parts of fermentation product of streptomyces griseofuscus, swelled ground, 10-18 parts of active enzyme, 20-25 parts of dispersing agent, 10-16 parts of glucose, 5-9 parts of Chinese medicine residue humus filtrate, 5-9 parts of vinegar and the balance of clear water.
4. The streptomyces griseofuscus bactericide as claimed in claim 1, wherein the streptomyces griseofuscus bactericide comprises the following components in parts by mass: 40 parts of fermentation product of streptomyces griseofuscus, bentonite, 12 parts of active enzyme, 20 parts of dispersing agent, 10 parts of glucose, 6 parts of Chinese medicine residue humus filtrate, 6 parts of vinegar and the balance of clear water.
5. The streptomyces griseofuscus bactericide as claimed in claim 1, wherein the streptomyces griseofuscus bactericide comprises the following components in parts by mass: 40 parts of fermentation product of streptomyces griseofuscus, bentonite, 12 parts of active enzyme, 25 parts of dispersing agent, 15 parts of glucose, 9 parts of Chinese medicine residue humus filtrate, 9 parts of vinegar and the balance of clear water.
6. The streptomyces griseofuscus bactericide as claimed in claim 1, wherein the preparation process of the Chinese medicine residue humus filtrate is as follows: filling the Chinese medicine residue into a container, adding clear water and cleaned large-particle stones, stirring to disperse the stones into the Chinese medicine residue, waiting, and filtering to obtain Chinese medicine residue humus filtrate after the Chinese medicine residue is rotten.
7. The streptomyces griseofuscus fungicide according to claim 1, characterized in that: the streptomyces griseofuscus is inoculated in a culture medium, the pH value is 7.0, the fermentation temperature is 36-39 ℃, the rotation speed is 180-.
8. The streptomyces griseofuscus fungicide according to claim 7, characterized in that: the inoculation amount of the activated strain in the culture process of the streptomyces griseofuscus strain is 6%.
9. A preparation method is realized based on the streptomyces griseofuscus bactericide of any one of claims 1 to 8, and is characterized by comprising the following steps:
the method comprises the following steps: adding active enzyme into the fermentation product of the streptomyces griseofuscus, uniformly mixing, and adding expansive soil to obtain a product A;
step two: uniformly mixing the Chinese medicine residue humus filtrate and glucose, and adding expansive soil to obtain a product B;
step three: mixing the product A and the product B, adding a proper amount of clear water and a dispersing agent, mixing again, and adding table vinegar after mixing uniformly;
step four: continuously adding expansive soil into the mixed solution, and drying and crushing the product;
step five: adding clear water into the crushed material, stirring uniformly, filtering to obtain filtrate and powder, and pressing the powder into granules to obtain granular bactericide and liquid bactericide.
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Application publication date: 20210917 |