CN113373122A - Preparation method of novel corn superoxide dismutase multienzyme - Google Patents
Preparation method of novel corn superoxide dismutase multienzyme Download PDFInfo
- Publication number
- CN113373122A CN113373122A CN202110863558.2A CN202110863558A CN113373122A CN 113373122 A CN113373122 A CN 113373122A CN 202110863558 A CN202110863558 A CN 202110863558A CN 113373122 A CN113373122 A CN 113373122A
- Authority
- CN
- China
- Prior art keywords
- corn
- superoxide dismutase
- multienzyme
- preparing
- filtrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 110
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 109
- 235000005822 corn Nutrition 0.000 title claims abstract description 109
- 102000019197 Superoxide Dismutase Human genes 0.000 title claims abstract description 64
- 108010012715 Superoxide dismutase Proteins 0.000 title claims abstract description 64
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 240000008042 Zea mays Species 0.000 title claims description 108
- 238000004140 cleaning Methods 0.000 claims abstract description 25
- 230000035784 germination Effects 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000001035 drying Methods 0.000 claims abstract description 21
- 238000002791 soaking Methods 0.000 claims abstract description 20
- 238000004537 pulping Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 28
- 239000000706 filtrate Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 16
- 208000003643 Callosities Diseases 0.000 claims description 14
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 14
- 238000001704 evaporation Methods 0.000 claims description 12
- 238000005507 spraying Methods 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- 102000016938 Catalase Human genes 0.000 claims description 9
- 239000003963 antioxidant agent Substances 0.000 claims description 9
- 230000003078 antioxidant effect Effects 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 8
- 235000006708 antioxidants Nutrition 0.000 claims description 8
- 239000012535 impurity Substances 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 102000006587 Glutathione peroxidase Human genes 0.000 claims description 7
- 108700016172 Glutathione peroxidases Proteins 0.000 claims description 7
- 239000008213 purified water Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 108010053835 Catalase Proteins 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 150000004676 glycans Chemical class 0.000 claims description 5
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 5
- 235000013824 polyphenols Nutrition 0.000 claims description 5
- 229920001282 polysaccharide Polymers 0.000 claims description 5
- 239000005017 polysaccharide Substances 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 4
- 229930003268 Vitamin C Natural products 0.000 claims description 4
- 235000019154 vitamin C Nutrition 0.000 claims description 4
- 239000011718 vitamin C Substances 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 abstract description 32
- 108090000790 Enzymes Proteins 0.000 abstract description 32
- 230000000694 effects Effects 0.000 abstract description 10
- 150000001875 compounds Chemical class 0.000 abstract description 9
- 238000009776 industrial production Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 241000209149 Zea Species 0.000 abstract 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
- 239000000047 product Substances 0.000 description 4
- 150000003254 radicals Chemical class 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 108030002440 Catalase peroxidases Proteins 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- -1 superoxide anions Chemical class 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000010718 Oxidation Activity Effects 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 206010057040 Temperature intolerance Diseases 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000008543 heat sensitivity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
- C12Y111/01—Peroxidases (1.11.1)
- C12Y111/01006—Catalase (1.11.1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
- C12Y111/01—Peroxidases (1.11.1)
- C12Y111/01009—Glutathione peroxidase (1.11.1.9)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y115/00—Oxidoreductases acting on superoxide as acceptor (1.15)
- C12Y115/01—Oxidoreductases acting on superoxide as acceptor (1.15) with NAD or NADP as acceptor (1.15.1)
- C12Y115/01001—Superoxide dismutase (1.15.1.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Environmental Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of a novel corn superoxide dismutase multienzyme, and relates to the technical field of preparation of the superoxide dismutase multienzyme. The invention comprises the steps of material preparation, germination, pulping, concentration, drying and the like. The invention selects the corn in Liaoning area, the compound enzyme content is high; the full-automatic seed cleaning, soaking and germinating device is adopted, the treatment capacity is large, the seeds can germinate for 2t each time, the labor intensity is reduced, the buds are uniform in length, the content of the compound enzyme is higher than that of the compound enzyme in the common germinating mode, and large-scale industrial production can be realized; and (3) removing water by adopting a low-temperature vacuum concentrator at the temperature of 22-25 ℃ and concentrating, wherein the yield of the activity of the compound enzyme is higher and the concentration multiple is higher at the temperature. Experiments show that the product of the invention is stored at 45 ℃. The enzyme activity is not reduced within 90 days, but is not reduced within 48 months when the product is stored at room temperature.
Description
Technical Field
The invention relates to the technical field of production of superoxide dismutase multienzyme, in particular to a preparation method of novel corn superoxide dismutase multienzyme.
Background
The superoxide dismutase multienzyme has the main effects of effectively removing superoxide anions, namely free radicals, in a human body, stimulating cell proliferation change and genetic factor mutation by excessive free radicals, and can cause various diseases. Reduce cell damage or death in vivo, thereby reducing disease occurrence and delaying aging. The existing methods for producing superoxide dismutase multienzyme mainly comprise two types, one is extracted from animal viscera, and the other is extracted from plants such as corn and the like. The existing method for preparing the superoxide dismutase complex enzyme by using the corn has many methods, but the problem of using chemical disinfectants such as bleaching powder, sodium hypochlorite and the like exists in the preparation process, and the chemical disinfectants are easy to remain and pollute the environment in the production process, so that the quality of the prepared superoxide dismutase complex enzyme is also influenced to a certain extent. Meanwhile, in the process of drying products, the existing method adopts freeze drying or spraying, the cost of the former is high, the enzyme activity loss of the latter is large, and the former and the latter are not suitable for the requirement of large-scale production. The existing method also has the technical problems of low germination rate, low enzyme yield, small production scale and the like, and because the corns germinate in the box body of the germination device, the ventilation quantity is small, the corn growth environment is narrow, the surfaces of the corns are easy to generate bacterial membranes, the rotten phenomenon is easy to occur, the germination rate is reduced, the quality of the prepared superoxide dismutase is influenced, residues are easy to occur, the superoxide dismutase is not easy to clean, and the labor intensity of workers is increased.
The invention relates to a Chinese patent with the patent number of CN201210160871.0, which is an industrial production method for preparing superoxide dismutase compound enzyme by using corn as a raw material and a germination room thereof, and solves the technical problems, but the Chinese patent also has the following technical problems: the germination rate and yield are low, the concentration efficiency is low, the type of the specifically produced complex enzyme is not clearly shown, and the quality guarantee period of the complex enzyme at high temperature is not shown.
Disclosure of Invention
In order to solve the problems, namely the problems brought forward by the background technology, the invention provides a preparation method of a novel corn superoxide dismutase multienzyme, and the specific technical scheme is as follows:
a preparation method of novel corn superoxide dismutase multienzyme is characterized in that: the production method comprises the following steps:
preparing materials: preparing a proper amount of corn serving as a raw material, and removing impurities in the corn for later use;
and (3) germination: placing the corn into a full-automatic seed cleaning, soaking and germinating device, cleaning, soaking for 6-15 hours, and germinating for 24-64 hours until the bud length is 3-5 mm;
pulping: adding purified water into the germinated corns according to the proportion of 1: 2-4, uniformly mixing and crushing the mixture into corn steep liquor, uniformly stirring the corn steep liquor, filtering the mixture, collecting filtrate, and adding an antioxidant which accounts for 0.5-1.5% of the weight of the filtrate into the filtrate;
concentrating and drying: putting the filtrate into a low-temperature vacuum concentrator, evaporating for 8-15 hours at 22-25 ℃ to obtain corn superoxide dismutase multienzyme liquid, uniformly mixing the corn superoxide dismutase multienzyme liquid and an active carrier, putting the mixture into a drying oven, and drying for 6-10 hours to obtain corn superoxide dismutase multienzyme powder.
Further, the production method comprises the following steps:
preparing materials: preparing a proper amount of corn, and removing impurities in the corn for later use;
and (3) germination: placing the corn into a full-automatic seed cleaning, soaking and germinating device, cleaning, soaking for 10 hours, and germinating for 50 hours until the bud length is 4 mm;
pulping: adding purified water into germinated corn according to the proportion of 1: 3, uniformly mixing and crushing the mixture into corn steep liquor, uniformly stirring the corn steep liquor, filtering, collecting filtrate, and adding an antioxidant which accounts for 1% of the weight of the filtrate into the filtrate;
concentrating and drying: putting the filtrate into a low-temperature vacuum concentrator, evaporating for 12 hours at the temperature of 22-25 ℃ to obtain corn superoxide dismutase multienzyme liquid, uniformly mixing the corn superoxide dismutase multienzyme liquid and an active carrier, putting the mixture into a drying oven, and drying for 8 hours to obtain corn superoxide dismutase multienzyme powder.
Further, the corn is corn in Liaoning area of producing area.
Further, the germination process specifically comprises: placing corn seeds into a full-automatic seed cleaning, soaking and germinating device, setting the temperature at 28-35 ℃ for germination, introducing compressed air into the device every 0.5 hour in a germination stage, spraying water into the device every 1 hour, draining the water after each spraying, and cleaning the germinated corns for 3-5 times for later use after the germination is finished.
Further, the antioxidant is one or two of apple polyphenol and vitamin C.
Further, the active carrier is one or two of starch and polysaccharide.
Further, a plate-and-frame filter press is adopted in the filtering step in the pulping process.
Furthermore, a low-temperature vacuum concentration device with the evaporation temperature of 22-25 ℃ is adopted in the concentration step.
Further, the superoxide dismutase multienzyme is specifically superoxide dismutase, glutathione peroxidase and catalase.
The beneficial technical effects of the invention are as follows: the special Liaoning area corn is selected, and the complex enzyme content is high.
Express in patent CN201210160871.0 and sprout the room simple structure, the air volume is big, and the maize percentage of sprouting is high, compares with patent CN201210160871.0, and this application adopts full-automatic seed to wash and soaks, the device that sprouts, and the handling capacity is big, and every jar can sprout 2t at every turn, reduces intensity of labour, and the bud is long even, and compound enzyme content is high than ordinary mode of sprouting. The equipment can complete the whole processes of corn cleaning, soaking, sprouting and the like, has high automation degree, low energy consumption, small occupied area and large processing capacity, and is particularly suitable for the requirement of the industrialized mass production of the corn superoxide dismutase multienzyme. When cleaning, the blast air is used for rolling and cleaning the corn, and floating impurities are automatically removed through overflow. When in soaking, the proper soaking water temperature can be controlled. During germination, the corns can be ventilated in a staggered mode through compressed air ports at different positions, so that good ventilation among the germinated corns is kept, and microorganisms are not easy to breed; meanwhile, the proper germination temperature and moisture are maintained by controlling the temperature of the spray water and the temperature of the germination tank body, so that the temperature and humidity conditions of the whole tank of corn are uniform, the germination rate is high, and the sprouts are uniform.
The water is removed by a low-temperature vacuum concentrator at the temperature of 22-25 ℃, the concentration is carried out, the evaporation efficiency is high and can reach 800L/h, and at the temperature, the yield of the activity of the compound enzyme is higher and the concentration multiple is higher. The evaporation temperature of the traditional reduced pressure concentration equipment is above 50 ℃, and partial temperature-sensitive bioactive substances such as enzymes and the like are easy to inactivate at the temperature, such as catalase and glutathione peroxidase, even superoxide dismutase with good thermal stability, and the yield is low. The low-temperature vacuum concentration equipment adopted by the application can be used for concentrating at the temperature of 22-25 ℃, so that not only can the inactivation of catalase and glutathione peroxidase be ensured, but also the yield of superoxide dismutase can be improved. Meanwhile, the concentration multiple of the device is higher and can reach 40-60 times, and the activity of the corn superoxide dismutase multienzyme liquid and powder in unit weight can be greatly improved under the condition of the same extraction rate. Finally, the equipment adopts a heat pump technology to provide a heat source and a cold source, thereby achieving the purposes of improving the efficiency, reducing the energy consumption and saving the space.
Experiments show that the product of the invention is stored at 45 ℃. The enzyme activity is not reduced within 90 days, but is not reduced within 48 months when the product is stored at room temperature.
Compared with the patent number of CN201210160871.0, the invention provides the industrial production method for preparing the superoxide dismutase complex enzyme by taking the corn as the raw material and the patent of a germination chamber, the application reduces the use of additives, only apple polyphenol and vitamin C are added as antioxidants and starch or polysaccharide is added as a carrier in the use process, and the rest is taken from the corn, so that the use of phosphate, diatomite and algal polysaccharide in the patent CN201210160871.0 is reduced, and the cost of the product is greatly reduced.
Meanwhile, as the byproducts are few, only a certain amount of byproducts of corn steep liquor filter residues exist in the pulping step, and the byproducts can be used as feed raw materials, the meat flavor can be improved, and the environmental pollution is reduced.
Detailed Description
Preferred embodiments of the present invention are described below. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and are not intended to limit the scope of the present invention.
Example 1
Preparing 2000kg of corn, and removing impurities in the corn for later use; placing the corns in a full-automatic seed cleaning, soaking and germinating device, cleaning, soaking for 6 hours, setting the temperature at 28-35 ℃, germinating for 24 hours, introducing compressed air into the device every 0.5 hour in a germinating stage, spraying water into the device every 1 hour, draining the water after each spraying, germinating until the bud length is 3mm, and cleaning the germinated corns for 3-5 times for later use. Adding purified water into germinated corn at a ratio of 1: 3, mixing, pulverizing into corn steep liquor, stirring, filtering, collecting filtrate, and adding apple polyphenol 0.5% of the filtrate weight. Putting the filtrate into a low-temperature vacuum concentrator, evaporating for 8 hours at the temperature of 22-25 ℃ to obtain corn superoxide dismutase multienzyme liquid, uniformly mixing the corn superoxide dismutase multienzyme liquid with starch, putting the mixture into a drying oven, and drying for 6 hours to obtain corn superoxide dismutase multienzyme powder.
Example 2
Preparing 2000kg of corn, and removing impurities in the corn for later use; placing the corns in a full-automatic seed cleaning, soaking and germinating device, cleaning, soaking for 10 hours, setting the temperature at 28-35 ℃, germinating for 50 hours, introducing compressed air into the device every 0.5 hour in a germinating stage, spraying water into the device every 1 hour, draining the water after each spraying, germinating until the bud length is 4mm, and cleaning the germinated corns for 3-5 times for later use. Adding purified water into germinated corn according to the proportion of 1: 3, uniformly mixing and crushing the mixture into corn steep liquor, uniformly stirring the corn steep liquor, filtering, collecting filtrate, and adding 1% of vitamin C in the weight of the filtrate into the filtrate. Putting the filtrate into a low-temperature vacuum concentrator, evaporating for 12 hours at the temperature of 22-25 ℃ to obtain corn superoxide dismutase multienzyme liquid, uniformly mixing the corn superoxide dismutase multienzyme liquid and polysaccharide, putting the mixture into a drying oven, and drying for 8 hours to obtain corn superoxide dismutase multienzyme powder.
Example 3
Preparing 2000kg of corn, and removing impurities in the corn for later use; placing the corns in a full-automatic seed cleaning, soaking and germinating device, cleaning, soaking for 15 hours, setting the temperature at 28-35 ℃, germinating for 64 hours, introducing compressed air into the device every 0.5 hour in a germinating stage, spraying water into the device every 1 hour, draining the water after each spraying, germinating until the bud length is 5mm, and cleaning the germinated corns for 3-5 times for later use. Adding purified water into germinated corn at a ratio of 1: 3, mixing, pulverizing into corn steep liquor, stirring, filtering, collecting filtrate, and adding apple polyphenol 1.5% of the filtrate. Putting the filtrate into a low-temperature vacuum concentrator, evaporating for 15 hours at the temperature of 22-25 ℃ to obtain corn superoxide dismutase multienzyme liquid, uniformly mixing the corn superoxide dismutase multienzyme liquid with starch, putting the mixture into a drying oven, and drying for 10 hours to obtain corn superoxide dismutase multienzyme powder.
During the germination process of corn seeds, a large amount of antioxidant enzyme substances are generated, wherein superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and Catalase (CAT) are the most important three enzymes, and the three enzymes have synergistic action and can complete the whole process of removing oxygen radicals, and the whole process is complete and efficient. The corn superoxide dismutase multienzyme is rich in the three enzymes.
Superoxide dismutase (SOD) for converting superoxide anion oxygen free radical
Conversion to hydrogen peroxide (H)2O2) And oxygen (O)2). And hydrogen peroxide (H)2O2) Still has strong oxidative activity, easily generates free radicals, and converts hydrogen peroxide (H) into hydrogen peroxide (H) by Catalase (CAT) and glutathione peroxidase (GSH-px)2O2) Converted into water without oxidation activity, and the whole process of removing free radicals is completed.
The three enzymes act synergistically to complete the whole process of free radical scavenging and antioxidation. Only one or two enzymes can not complete the whole process, and the antioxidant effect is influenced.
The two enzymes, catalase and glutathione peroxidase, are relatively sensitive to temperature and easily inactivated in a liquid state with a relatively low concentration. Therefore, the two enzymes are not described in other inventions of corn superoxide dismutase multienzyme, because the evaporation temperature is generally above 50 ℃ by adopting a decompression concentration device. The low-temperature vacuum concentration equipment can carry out high-efficiency concentration at the temperature of 22-25 ℃, can protect the activity of the two enzymes in the heat sensitivity stage to the maximum extent, and has good heat stability under the protection of an antioxidant when the two enzymes are concentrated by more than 30 times, thereby facilitating the subsequent drying process.
The corn superoxide dismutase multienzyme powder obtained in the examples 1-3 and the superoxide dismutase complex enzyme obtained in the patent CN201210160871.0 are compared and tested, and the test results are as follows:
the experimental result shows that compared with the superoxide dismutase compound enzyme preparation obtained in the patent CN201210160871.0, the superoxide dismutase compound enzyme powder obtained in the patent has better shelf life and enzyme activity. And under the same conditions, example 3 works best.
While the invention has been described with reference to a preferred embodiment, various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention, and particularly, features shown in the various embodiments may be combined in any suitable manner without departing from the scope of the invention. It is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
The preferred embodiments shown thus far describe the technical solutions of the present invention, but it is obvious to those skilled in the art that the scope of the present invention is not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.
Claims (9)
1. A preparation method of novel corn superoxide dismutase multienzyme is characterized in that: the production method comprises the following steps:
preparing materials: preparing a proper amount of corn serving as a raw material, and removing impurities in the corn for later use;
and (3) germination: placing the corn into a full-automatic seed cleaning, soaking and germinating device, cleaning, soaking for 6-15 hours, and germinating for 24-64 hours until the bud length is 3-5 mm;
pulping: adding purified water into the germinated corns according to the proportion of 1: 2-4, uniformly mixing and crushing the mixture into corn steep liquor, uniformly stirring the corn steep liquor, filtering the mixture, collecting filtrate, and adding an antioxidant which accounts for 0.5-1.5% of the weight of the filtrate into the filtrate;
concentrating and drying: putting the filtrate into a low-temperature vacuum concentrator, evaporating for 8-15 hours at 22-25 ℃ to obtain corn superoxide dismutase multienzyme liquid, uniformly mixing the corn superoxide dismutase multienzyme liquid and an active carrier, putting the mixture into a drying oven, and drying for 6-10 hours to obtain corn superoxide dismutase multienzyme powder.
2. The method for preparing the novel corn superoxide dismutase multienzyme according to claim 1, wherein: the production method comprises the following steps:
preparing materials: preparing a proper amount of corn, and removing impurities in the corn for later use;
and (3) germination: placing the corn into a full-automatic seed cleaning, soaking and germinating device, cleaning, soaking for 10 hours, and germinating for 50 hours until the bud length is 4 mm;
pulping: adding purified water into germinated corn according to the proportion of 1: 3, uniformly mixing and crushing the mixture into corn steep liquor, uniformly stirring the corn steep liquor, filtering, collecting filtrate, and adding an antioxidant which accounts for 1% of the weight of the filtrate into the filtrate;
concentrating and drying: putting the filtrate into a low-temperature vacuum concentrator, evaporating for 12 hours at the temperature of 22-25 ℃ to obtain corn superoxide dismutase multienzyme liquid, uniformly mixing the corn superoxide dismutase multienzyme liquid and an active carrier, putting the mixture into a drying oven, and drying for 8 hours to obtain corn superoxide dismutase multienzyme powder.
3. The method for preparing a novel corn superoxide dismutase multienzyme as claimed in claim 1 or 2, characterized in that: the corn is corn in Liaoning area of producing area.
4. The method for preparing a novel corn superoxide dismutase multienzyme as claimed in claim 1 or 2, characterized in that: the germination process specifically comprises the following steps: placing corn seeds into a full-automatic seed cleaning, soaking and germinating device, setting the temperature at 28-35 ℃ for germination, introducing compressed air into the device every 0.5 hour in a germination stage, spraying water into the device every 1 hour, draining the water after each spraying, and cleaning the germinated corns for 3-5 times for later use after the germination is finished.
5. The method for preparing a novel corn superoxide dismutase multienzyme as claimed in claim 1 or 2, characterized in that: the antioxidant is one or two of apple polyphenol and vitamin C.
6. The method for preparing a novel corn superoxide dismutase multienzyme as claimed in claim 1 or 2, characterized in that: the active carrier is one or two of starch and polysaccharide.
7. The method for preparing a novel corn superoxide dismutase multienzyme as claimed in claim 1 or 2, characterized in that: and a plate-and-frame filter press is adopted in the filtering step in the pulping process.
8. The method for preparing the novel corn superoxide dismutase multienzyme according to claim 7, wherein: in the concentration step, low-temperature vacuum concentration equipment with the evaporation temperature of 22-25 ℃ is adopted.
9. The method for preparing a novel corn superoxide dismutase multienzyme as claimed in claim 1 or 2, characterized in that: the superoxide dismutase multienzyme is specifically superoxide dismutase, glutathione peroxidase, and catalase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110863558.2A CN113373122A (en) | 2021-07-29 | 2021-07-29 | Preparation method of novel corn superoxide dismutase multienzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110863558.2A CN113373122A (en) | 2021-07-29 | 2021-07-29 | Preparation method of novel corn superoxide dismutase multienzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113373122A true CN113373122A (en) | 2021-09-10 |
Family
ID=77583046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110863558.2A Pending CN113373122A (en) | 2021-07-29 | 2021-07-29 | Preparation method of novel corn superoxide dismutase multienzyme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113373122A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5904921A (en) * | 1996-04-03 | 1999-05-18 | Coletica | Stabilized compositions of superoxide dismutase obtained from germinated plant seeds |
| CN1224060A (en) * | 1999-01-07 | 1999-07-28 | 王元志 | Method for large scale production of corn superoxide dismutase (SOD) complex enzyme |
| CN1478889A (en) * | 2003-06-20 | 2004-03-03 | 王建英 | Production method of corn superoxide dismutase multienzyme |
| CN1513988A (en) * | 2003-07-09 | 2004-07-21 | 王建英 | Production method of corn superoxide dismutase multienzyme |
| CN1570095A (en) * | 2004-05-09 | 2005-01-26 | 沈阳五爱康田生物制品有限责任公司 | Superoxide dismutase complex enzyme industrialized production method using corn as material and its special device for germination |
| CN103281903A (en) * | 2010-05-26 | 2013-09-04 | 雀巢产品技术援助有限公司 | Methods for increasing the production or activity of catalase |
| CN103421750A (en) * | 2012-05-23 | 2013-12-04 | 王兆非 | Industrial production method for preparing superoxide dismutase compound enzymes with corn as raw material and sprouting room |
-
2021
- 2021-07-29 CN CN202110863558.2A patent/CN113373122A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5904921A (en) * | 1996-04-03 | 1999-05-18 | Coletica | Stabilized compositions of superoxide dismutase obtained from germinated plant seeds |
| CN1224060A (en) * | 1999-01-07 | 1999-07-28 | 王元志 | Method for large scale production of corn superoxide dismutase (SOD) complex enzyme |
| CN1478889A (en) * | 2003-06-20 | 2004-03-03 | 王建英 | Production method of corn superoxide dismutase multienzyme |
| CN1513988A (en) * | 2003-07-09 | 2004-07-21 | 王建英 | Production method of corn superoxide dismutase multienzyme |
| CN1570095A (en) * | 2004-05-09 | 2005-01-26 | 沈阳五爱康田生物制品有限责任公司 | Superoxide dismutase complex enzyme industrialized production method using corn as material and its special device for germination |
| CN103281903A (en) * | 2010-05-26 | 2013-09-04 | 雀巢产品技术援助有限公司 | Methods for increasing the production or activity of catalase |
| CN103421750A (en) * | 2012-05-23 | 2013-12-04 | 王兆非 | Industrial production method for preparing superoxide dismutase compound enzymes with corn as raw material and sprouting room |
Non-Patent Citations (1)
| Title |
|---|
| 王海灵: "玉米超氧化物歧化酶（SOD）提取及纯化技术的研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》, no. 03, pages 016 - 228 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110199610B (en) | Production method of tartary buckwheat sprouts | |
| CN106748195A (en) | A kind of fertilizer and its preparation technology containing natural plants growth substance | |
| CN109370925A (en) | A kind of solid fermentation culture medium of Paecilomyces lilacinus and the cultural method of Paecilomyces lilacinus | |
| CN107032877A (en) | A kind of efficient implantation methods of selenium-rich passion fruit | |
| EP3459360A1 (en) | A method for producing a paste with a high content of nutrients from germinated grain | |
| CN111543137B (en) | Rose seed seedling raising method | |
| CN108668557A (en) | It is a kind of to utilize method of germination corn enrichment lutein and products thereof and application | |
| CN113373122A (en) | Preparation method of novel corn superoxide dismutase multienzyme | |
| KR20110022383A (en) | Fertilizer composition for green tea, and manufacturing method thereof | |
| CN105850271A (en) | Processing technology of chromium-rich malt flour | |
| CN105285726A (en) | Wheat germ powder with high organic selenium content and processing technology of wheat germ powder | |
| CN110881607A (en) | Production technology of full-bud wheat steamed bread rich in gamma-aminobutyric acid | |
| KR101389583B1 (en) | Method for detoxing and steaming flaxseeds | |
| CN110810249B (en) | Culture medium for promoting elongation of oriental blueberry cluster buds as well as preparation method and application of culture medium | |
| CN109287460B (en) | Preparation method of bean sprouts rich in selenium | |
| CN114503989A (en) | Application of 2-amino-3-indolyl butyric acid in promoting plant growth | |
| CN106612706A (en) | Method for breaking dormancy of corn seeds | |
| KR20020069704A (en) | The vingar made from the unpolished rice and the ginseng extract etc. and it's manufacturing method. | |
| CN112979361A (en) | Soilless culture nutrition gel | |
| CN1140482C (en) | Method for preparing resisting growth regulator of red alga polyphenols | |
| CN110800421A (en) | Method for improving germination rate of cistanche seeds | |
| CN116024061B (en) | Tartary buckwheat yellow wine rich in ferulic acid and preparation method thereof | |
| CN1416670A (en) | Biological synthesis process of Se compound | |
| CN107242129A (en) | A kind of cuttage seedling raising method of peach rapid field planting | |
| LU101609B1 (en) | Method for enriching lutein and zeaxanthin by utilizing germinated corns |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |