CN113353429A - Reagent tube storage device for gene detection and storage method thereof - Google Patents
Reagent tube storage device for gene detection and storage method thereof Download PDFInfo
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- CN113353429A CN113353429A CN202110513001.6A CN202110513001A CN113353429A CN 113353429 A CN113353429 A CN 113353429A CN 202110513001 A CN202110513001 A CN 202110513001A CN 113353429 A CN113353429 A CN 113353429A
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
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- B65D25/101—Springs, elastic lips, or other resilient elements to locate the articles by pressure
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- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
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- B65D51/00—Closures not otherwise provided for
- B65D51/24—Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes
- B65D51/26—Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes with means for keeping contents in position, e.g. resilient means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
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- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention discloses a reagent tube storage device for gene detection and a storage method thereof. This gene detects uses reagent pipe storage device drives reagent pipe through electric telescopic handle and goes up and down, and then vibrates the inside sample of reagent pipe, makes sample solution and cell preservation liquid misce bene, effectively preserves more cells that have the testing value, and when freezing or unfreezing, effectively avoids local to be refrigerated or heated, avoids the cell activity to receive the destruction.
Description
Technical Field
The invention relates to the technical field of gene detection, in particular to a reagent tube storage device for gene detection and a storage method thereof.
Background
The gene detection is a technology for detecting DNA through blood, other body fluids or cells, which takes peripheral venous blood or other tissue cells of a detected person, amplifies the gene information, detects DNA molecular information in the cells of the detected person through a specific device, analyzes various conditions of the gene contained in the cells, and accordingly enables people to know the gene information of the people, and to determine the cause of disease or predict the risk of the body to suffer from certain diseases. When the extracted gene sample needs to be transported to other places for detection, the reagent tube filled with the gene sample needs to be placed in a special storage device for freezing and storage, the activity of the sample cells is maintained, and the sample cells are thawed when the detection is carried out, so that the temperature of the sample is recovered to the room temperature.
The existing reagent tube storage device can only store reagent tubes with fixed sizes, the reagent tubes can only keep a static state when being placed in the device, gene samples need to be uniformly mixed with corresponding cell preservation solution before being frozen and preserved, effective cells embedded in sample mucus are separated out, more cells with detection values are effectively preserved, and in the prior art, the mixed solution is shaken up manually, so that time and labor are wasted, and the operation is inconvenient; in addition, when a sample is frozen and stored, if the sample mixture is kept still all the time, the freezing is not uniform, crystals are likely to precipitate in the reagent tube, and when the sample mixture is thawed and returned to room temperature, if the sample mixture is kept still all the time, the sample is locally heated, and the cell activity is destroyed.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a reagent tube storage device for gene detection, which has the advantages of capability of vibrating a reagent tube, capability of storing reagent tubes with different specifications and the like, and solves the problem that a sample mixed liquid is kept still all the time and only the reagent tubes with fixed sizes can be stored.
(II) technical scheme
In order to solve the technical problems that the sample mixed liquid is kept still all the time and only a reagent tube with a fixed size can be stored, the invention provides the following technical scheme:
a reagent tube storage device for gene detection comprises a box body, wherein heat dissipation holes are formed in the surfaces of two sides of the box body in a penetrating mode, supporting plates are arranged on two sides inside the box body respectively, a controller, a cold water storage tank and a hot water storage tank are arranged on the bottom surface inside the box body, the cold water storage tank and the hot water storage tank are arranged on one sides, close to each other, of the two supporting plates respectively, the controller is arranged in a region between the cold water storage tank and the hot water storage tank, a water inlet pipe and a water outlet pipe are arranged on one sides, close to each other, of the cold water storage tank and the hot water storage tank respectively, a water pump is arranged on the water inlet side of the water inlet pipe and the water outlet side of the water outlet pipe respectively, a water tank is arranged at the upper ends of the cold water storage tank and the hot water storage tank, the water inlet pipe and the water outlet pipe are communicated with the inside of the water tank, and a refrigerator and a heater are arranged on the bottom surface inside the water tank, the inside upper end of water tank is provided with a reagent pipe and places a section of thick bamboo, a reagent pipe is placed a section of thick bamboo evenly distributed, the reagent pipe place a section of thick bamboo outside bottom surface with the refrigerator with the heater is not contact each other, the inside upper end of water tank is provided with first holding tank and second holding tank, first holding tank with the equal upper end opening lower extreme of second holding tank seals.
Preferably, the first holding tank with the crossing department of second holding tank is linked together, first holding tank runs through respectively the reagent pipe places the upper end both sides of a section of thick bamboo, the second holding tank sets up respectively adjacent two the reagent pipe is placed between the section of thick bamboo, first holding tank both ends are provided with first bracing piece respectively, second holding tank both ends are provided with the second bracing piece respectively, first bracing piece with the second bracing piece links to each other and constitutes closed rectangle.
Preferably, first bracing piece with the second bracing piece sets up the box inner wall with in the region that water tank outer wall formed, two fixedly connected with connecting rod between the second bracing piece, the connecting rod sets up inside the second holding tank, first bracing piece with second bracing piece handing-over department lower surface is provided with electric telescopic handle, electric telescopic handle fixed mounting be in backup pad up end both sides.
Preferably, a sealing plate is fixedly mounted at the upper end of the water tank, a round hole penetrates through the surface of the sealing plate, the round hole corresponds to the reagent tube placing cylinder in position and size, connecting blocks are fixedly connected to two sides of the connecting rod and one side, close to each other, of the first supporting rod, the connecting blocks are arranged inside the first accommodating groove, and connecting plates are fixedly connected to the tail ends of the connecting blocks respectively.
Preferably, the connecting plates are respectively arranged inside the reagent tube containing barrel, the connecting plates are located in the same position, the two connecting plates inside the reagent tube containing barrel are fixedly provided with a first spring at the lower end of one side close to each other respectively, a telescopic guide rod is sleeved inside the first spring, and the tail end of the first spring is fixedly provided with an arc-shaped clamping plate respectively.
Preferably, arc grip block medial surface is provided with the protection cushion respectively, is located same the reagent pipe is placed a section of thick bamboo inside two be provided with the reagent pipe between the arc grip block, the reagent pipe includes pipe shaft and tube cap, the tube cap sets up pipe shaft upper end, the tube cap lower surface with arc grip block up end parallel and level.
Preferably, there is the case lid box upper end through hinge movable mounting, the box with through buckle fixed connection between the case lid, case lid external surface is provided with control panel, the control panel both sides are provided with the fixed block respectively, the fixed block end is fixed mounting respectively has the second spring, the second spring outside is provided with movable sleeve, the outer wall of fixed block with movable sleeve's inner wall size phase-match.
Preferably, the second spring with movable sleeve's inner wall fixed connection, movable sleeve is close to control panel's one end upper surface is provided with the arm-tie, the fixed block is kept away from one side of control panel is provided with the mounting hole, the mounting hole sets up case lid surface both sides, the inside spliced pole that runs through respectively of mounting hole is provided with, the spliced pole side runs through respectively and is provided with first locating hole and second locating hole.
Preferably, first locating hole with the second locating hole all with movable sleeve's outer wall size phase-match, the second locating hole sets up first locating hole lower extreme, spliced pole upper end fixedly connected with antiskid handle, spliced pole lower extreme fixed mounting has the mounting panel, the mounting panel sets up inside the case lid, mounting panel fixed surface installs the third spring, the third spring end is provided with circular clamp plate respectively.
Preferably, the surface of the mounting plate is fixedly provided with an ultraviolet lamp, one of the ultraviolet lamps is arranged on the surface of the inner wall of the reagent tube placing barrel, and the water pump, the refrigerator, the heater, the electric telescopic rod, the ultraviolet lamp and the temperature sensor are all connected with the controller and the control panel through electric signals.
(III) advantageous effects
Compared with the prior art, the invention provides a reagent tube storage device for gene detection, which has the following beneficial effects:
1. this gene detects uses reagent pipe storage device drives first bracing piece and the quick reciprocal lift of second bracing piece through electric telescopic handle for the connecting rod goes up and down in that the second holding tank is inside, and then drives the connecting block and goes up and down in that first holding tank is inside, makes the arc grip block place a section of thick bamboo inside going up and down at the reagent pipe, finally drives the reagent pipe and goes up and down, and then vibrates the inside sample of reagent pipe.
2. This gene detects uses reagent pipe storage device drives reagent pipe fast lift through electric telescopic handle, makes sample solution and cell preservation liquid misce bene, separates the effective cell of embedding in the sample mucus, effectively preserves more cells that have the testing value, provides sufficient cell quantity, provides the assurance for the accuracy of testing result.
3. This gene detects uses reagent pipe storage device drives reagent pipe through electric telescopic handle and slowly goes up and down, makes the mixed liquid of reagent intraduct vibrate slightly, when cooling down freezing to the mixed liquid of sample of inside for it is even to freeze, avoids appearing the crystal, when heating up the heating to the mixed liquid of sample of inside, makes the heating even, avoids the part to be heated, destroys the cell activity.
4. This gene detects uses reagent pipe storage device, through carrying the water in the cold water storage box to inside the water tank, temperature sensor real-time supervision reagent pipe places the inside temperature of a section of thick bamboo, cool down through the refrigerator, make the reagent pipe place a section of thick bamboo inside temperature and reach the suitable preserved optimum temperature of gene sample of the inside storage of reagent pipe, the sample in the reagent pipe needs to resume room temperature and detects time measuring, carry the inside frozen water of water tank to in the cold water storage box, carry the normal atmospheric temperature water in the hot water storage box to in the water tank, heat up through the heater, make the reagent pipe place a section of thick bamboo inside temperature and reach the room temperature, temperature difference when reducing refrigeration or heating, can reach the refrigeration or heating effect in the short time, and energy is saved.
5. This gene detects uses reagent pipe storage device carries out the centre gripping to the body through arc grip block, and the tube cap carries on spacingly in the arc grip block upper end, avoids reagent pipe landing between two arc grip blocks, and in electric telescopic handle's the biggest telescopic distance, the gene of any size of arc grip block centre gripping detects uses reagent pipe and can not place the bottom surface contact of a section of thick bamboo with reagent pipe for the arc grip block can the centre gripping different length and the reagent pipe of different bore sizes, improves the practicality of device.
6. According to the reagent tube storage device for gene detection, the mounting plate can be driven to lift by pulling or pressing the anti-slip handle, so that the circular pressing plate is driven to lift, the movable sleeve is inserted into the first positioning hole or the second positioning hole to position the mounting plate, when the movable sleeve is inserted into the second positioning hole, the circular pressing plate is far away from the reagent tube placing barrel, when the movable sleeve is inserted into the first positioning hole, the circular pressing plate stretches into the reagent tube placing barrel and is always pressed on the upper end face of the reagent tube, and the phenomenon that the arc-shaped clamping plate directly slides down along the outer surface of the tube body when lifting is avoided;
7. this gene detects uses reagent pipe storage device through having the ultraviolet lamp at mounting panel fixed surface, inside movable sleeve inserted the second locating hole, circular clamp plate kept away from the reagent pipe and placed a section of thick bamboo, opened the ultraviolet lamp and can place a section of thick bamboo inside and the closing plate surface is disinfected to the reagent pipe.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a second schematic perspective view of the present invention;
FIG. 3 is a schematic cross-sectional view of the present invention;
FIG. 4 is a schematic view of the structure of the support rod, the arc-shaped holding plate and the reagent tube according to the present invention;
FIG. 5 is a schematic structural diagram of the case of the present invention;
FIG. 6 is a schematic view showing the construction of a cold water storage tank and a hot water storage tank according to the present invention;
FIG. 7 is a schematic view of the structure of the water tank of the present invention;
FIG. 8 is a schematic view of a support bar according to the present invention;
FIG. 9 is a schematic view of the arcuate clamping plate and reagent tube configuration of the present invention;
FIG. 10 is a schematic view of a connecting column according to the present invention;
fig. 11 is a schematic view of the structure of the case cover of the present invention.
In the figure: 1. a box body; 2. heat dissipation holes; 3. a support plate; 4. a controller; 5. a cold water storage tank; 6. a hot water storage tank; 7. a water inlet pipe; 8. a water outlet pipe; 9. a water pump; 10. a water tank; 11. a refrigerator; 12. a heater; 13. a reagent tube placement barrel; 14. a first accommodating groove; 15. a second accommodating groove; 16. a first support bar; 17. a second support bar; 18. a connecting rod; 19. an electric telescopic rod; 20. a sealing plate; 21. a circular hole; 22. connecting blocks; 23. a connecting plate; 24. a first spring; 25. an arc-shaped clamping plate; 26. a reagent tube; 261. a tube body; 262. a tube cover; 27. a box cover; 28. buckling; 29. a control panel; 30. a fixed block; 31. a second spring; 32. a movable sleeve; 33. pulling a plate; 34. mounting holes; 35. connecting columns; 36. a first positioning hole; 37. a second positioning hole; 38. an anti-slip handle; 39. mounting a plate; 40. a third spring; 41. a circular pressing plate; 42. an ultraviolet lamp; 43. a temperature sensor.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-11, a reagent tube storage device for gene detection comprises a box body 1, heat dissipation holes 2 are formed through the surfaces of both sides of the box body 1, support plates 3 are respectively arranged on both sides of the inside of the box body 1, a controller 4, a cold water storage tank 5 and a hot water storage tank 6 are arranged on the bottom surface of the inside of the box body 1, the cold water storage tank 5 and the hot water storage tank 6 are respectively arranged on the sides of the two support plates 3 close to each other, the controller 4 is arranged in the region between the cold water storage tank 5 and the hot water storage tank 6, a water inlet pipe 7 and a water outlet pipe 8 are respectively arranged on the sides of the cold water storage tank 5 and the hot water storage tank 6 close to each other, a water pump 9 is respectively arranged on the water inlet side of the water inlet pipe 7 and the water outlet side of the water outlet pipe 8, a water tank 10 is arranged at the upper ends of the cold water storage tank 5 and the hot water storage tank 6, the water inlet pipe 7 and the water outlet pipe 8 are both communicated with the inside of the water tank 10, a refrigerator 11 and a heater 12 are arranged on the bottom surface inside the water tank 10, a reagent pipe placing barrel 13 is arranged at the upper end inside the water tank 10, the reagent pipe placing barrel 13 is evenly distributed, the reagent pipe placing barrel 13 is externally arranged on the bottom surface of the refrigerator 11 and the heater 12 and is not in contact with each other, a first accommodating groove 14 and a second accommodating groove 15 are arranged at the upper end inside the water tank 10, and the lower ends of the upper end openings of the first accommodating groove 14 and the second accommodating groove 15 are sealed.
Further, the crossing department of first holding tank 14 and second holding tank 15 is linked together, first holding tank 14 runs through the upper end both sides that a section of thick bamboo 13 was placed to the reagent pipe respectively, second holding tank 15 sets up respectively and places between a section of thick bamboo 13 at two adjacent reagent pipes, first holding tank 14 both ends are provided with first bracing piece 16 respectively, second holding tank 15 both ends are provided with second bracing piece 17 respectively, first bracing piece 16 and second bracing piece 17 link to each other and constitute the closed rectangle, thereby first bracing piece 16 and second bracing piece 17 can slide along vertical direction at first holding tank 14 and second holding tank 15 both ends, and the inside water of water tank 10 is placed a section of thick bamboo 13 by the reagent pipe, first holding tank 14 and the 15 separation of second holding tank, can not get into the reagent pipe and place inside a section of thick bamboo 13.
Further, first bracing piece 16 and second bracing piece 17 set up in the region that 1 inner wall of box and water tank 10 outer wall formed, fixedly connected with connecting rod 18 between two second bracing pieces 17, connecting rod 18 sets up inside second holding tank 15, first bracing piece 16 and second bracing piece 17 handing-over department lower surface are provided with electric telescopic handle 19, 19 fixed mounting of electric telescopic handle are in 3 up end both sides in backup pad, thereby electric telescopic handle 19 is flexible can drive first bracing piece 16 and second bracing piece 17 and go up and down, and then drive connecting rod 18 and go up and down inside second holding tank 15.
Further, water tank 10 upper end fixed mounting has a closing plate 20, closing plate 20 surface runs through and is provided with round hole 21, round hole 21 and reagent pipe place a position of section of thick bamboo 13, the size is corresponding, the equal fixedly connected with connecting block 22 in one side that connecting rod 18 both sides and first bracing piece 16 are close to each other, connecting block 22 sets up inside first holding tank 14, connecting block 22 end fixedly connected with connecting plate 23 respectively, thereby electric telescopic handle 19 is flexible can drive connecting block 22 and go up and down in first holding tank 14 is inside. In addition, in a specific application, the maximum height that the connecting block 22 can be lifted inside the first accommodating groove 14 is equal to the maximum telescopic distance of the electric telescopic rod 19.
Further, the connecting plate 23 sets up respectively and places a section of thick bamboo 13 inside at the reagent pipe, it has first spring 24 to be located that one side lower extreme that two connecting plates 23 that a section of thick bamboo 13 is placed to same reagent pipe are close to each other respectively fixed mounting, first spring 24 is inside to have cup jointed flexible guide bar, 24 end fixed mounting respectively of first spring has arc grip block 25, thereby electric telescopic handle 19 is flexible to drive connecting plate 23 and places a section of thick bamboo 13 inside lift at the reagent pipe, and then drive the lift of arc grip block 25, and be located that article that can the centre gripping different length and different bore sizes between two inside arc grip blocks 25 of a section of thick bamboo 13 are placed to same reagent pipe. In addition, during specific application, the two arc-shaped clamping plates 25 positioned in the same reagent tube placing barrel 13 are opposite in arc surface direction.
Further, arc grip block 25 medial surface is provided with the protection cushion respectively, it is provided with reagent pipe 26 to be located that same reagent pipe places between two inside arc grip blocks 25 of a section of thick bamboo 13, thereby 19 flexible reagent pipes 26 that can drive go up and down of electric telescopic handle, and then vibrate the inside sample of reagent pipe 26, reagent pipe 26 includes pipe shaft 261 and tube cap 262, tube cap 262 sets up in pipe shaft 261 upper end, tube cap 262 lower surface and arc grip block 25 up end parallel and level, thereby tube cap 262 is spacing on its upper end when arc grip block 25 centre gripping pipe shaft 261, avoid reagent pipe 26 landing between two arc grip blocks 25. In addition, in a specific application, the reagent tube 26 for gene detection of any size held by the arc-shaped holding plate 25 does not contact the bottom surface of the reagent tube accommodating cylinder 13 within the maximum extension distance of the electric telescopic rod 19.
Further, a box cover 27 is movably mounted at the upper end of the box body 1 through a hinge, the box body 1 and the box cover 27 are fixedly connected through a buckle 28, a control panel 29 is arranged on the outer surface of the box cover 27, fixing blocks 30 are respectively arranged on two sides of the control panel 29, second springs 31 are respectively fixedly mounted at the tail ends of the fixing blocks 30, movable sleeves 32 are arranged on the outer sides of the second springs 31, and the outer walls of the fixing blocks 30 are matched with the inner walls of the movable sleeves 32 in size.
Further, the inner wall fixed connection of second spring 31 and movable sleeve 32, the one end upper surface that movable sleeve 32 is close to control panel 29 is provided with arm-tie 33, one side that control panel 29 was kept away from to fixed block 30 is provided with mounting hole 34, mounting hole 34 sets up in case lid 27 surface both sides, the inside spliced pole 35 that runs through respectively of mounting hole 34, spliced pole 35 side runs through respectively and is provided with first locating hole 36 and second locating hole 37, thereby pulling arm-tie 33 drives movable sleeve 32 and slides, make movable sleeve 32 cup joint at fixed block 30 outside, second spring 31 can be compressed, and then when loosening arm-tie 33, second spring 31 pops out and can drive movable sleeve 32 and reset. In addition, in specific application, when the second spring 31 is in a natural state, the movable sleeve 32 is just inserted into the first positioning hole 36 or the second positioning hole 37, and when the second spring 31 is completely compressed, the movable sleeve 32 is just completely separated from the first positioning hole 36 or the second positioning hole 37.
Further, first locating hole 36 and second locating hole 37 all match with movable sleeve 32's outer wall size, second locating hole 37 sets up at first locating hole 36 lower extreme, spliced pole 35 upper end fixedly connected with antiskid handle 38, spliced pole 35 lower extreme fixed mounting has mounting panel 39, mounting panel 39 sets up inside case lid 27, mounting panel 39 fixed surface installs third spring 40, third spring 40 end is provided with circular clamp plate 41 respectively, thereby the pulling or press antiskid handle 38 can drive mounting panel 39 and go up and down, and then drive circular clamp plate 41 and go up and down, movable sleeve 32 inserts first locating hole 36 or the inside mounting panel 39 that can fix a position of second locating hole 37. In addition, in specific application, the surface diameter of the circular pressing plate 41 is equal to the distance between the two connecting plates 23 located inside the same reagent tube placing cylinder 13, when the box cover 27 is closed at the upper end of the box body 1, the positions of the circular pressing plates 41 correspond to the positions of the reagent tube placing cylinder 13 one by one, and under the elastic action of the third spring 40, the circular pressing plate 41 is always pressed on the upper end face of the reagent tube 26, so that the arc-shaped clamping plate 25 is prevented from directly sliding down along the outer surface of the tube body 261 when being lifted; when the movable sleeve 32 is inserted into the second positioning hole 37, the circular pressing plate 41 is away from the reagent tube accommodating cylinder 13, and when the movable sleeve 32 is inserted into the first positioning hole 36, the circular pressing plate 41 extends into the reagent tube accommodating cylinder 13 and is always pressed on the upper end surface of the reagent tube 26.
Further, the surface of the mounting plate 39 is fixedly provided with an ultraviolet lamp 42, so that the movable sleeve 32 is inserted into the second positioning hole 37, when the circular pressing plate 41 is far away from the reagent tube placing tube 13, the ultraviolet lamp 42 is turned on to sterilize the inside of the reagent tube placing tube 13 and the surface of the sealing plate 20, the surface of the inner wall of one of the reagent tube placing tubes 13 is provided with a temperature sensor 43, so that the temperature inside the reagent tube placing tube 13 can be monitored in real time, the water pump 9, the refrigerator 11, the heater 12, the electric telescopic rod 19, the ultraviolet lamp 42 and the temperature sensor 43 are all connected with the controller 4 and the control panel 29 through electric signals, so that the operation control panel 29 can control the water pump 9, the refrigerator 11, the heater 12, the electric telescopic rod 19, the ultraviolet lamp 42 and the temperature sensor 43 to work through the controller 4, when samples in the reagent tube 26 need to be frozen and stored, water in the cold water storage tank 5 is conveyed into the water tank 10, the temperature sensor 43 monitors the internal temperature of the reagent tube placing cylinder 13 in real time, and the temperature is reduced through the refrigerator 11, so that the internal temperature of the reagent tube placing cylinder 13 reaches the optimal temperature suitable for storing the gene sample stored in the reagent tube 26; when the sample in the reagent tube 26 needs to be restored to the room temperature for detection, the ice water in the water tank 10 is conveyed into the cold water storage tank 5, the normal temperature water in the hot water storage tank 6 is conveyed into the water tank 10, the temperature is raised through the heater 12, the temperature in the reagent tube accommodating cylinder 13 reaches the room temperature, and the sample can be thawed in a short time.
The working principle is as follows: when the kit is used, a proper amount of corresponding cell preservation solution is added into a reagent tube 26 filled with a gene sample, the sealed reagent tube 26 is directly placed inside a reagent tube placing cylinder 13, an arc-shaped clamping plate 25 clamps a tube body 261, a tube cover 262 is limited at the upper end of the arc-shaped clamping plate 25, the reagent tube 26 is prevented from sliding off the two arc-shaped clamping plates 25, the reagent tubes 26 with different lengths and different calibers can be clamped between the two arc-shaped clamping plates 25 inside the same reagent tube placing cylinder 13, and a box cover 27 is closed at the upper end of a box body 1 and locked through a buckle 28;
pulling the pulling plate 33 to drive the movable sleeve 32 to slide, compressing the second spring 31 to make the movable sleeve 32 completely separate from the first positioning hole 36, pressing the anti-skid handle 38 to drive the mounting plate 39 to descend, further driving the circular pressing plate 41 to descend, loosening the pulling plate 33, popping the second spring 31 to drive the movable sleeve 32 to reset, inserting the movable sleeve 32 into the first positioning hole 36 to position the mounting plate 39, pressing the circular pressing plate 41 on the upper end of the reagent tube 26 in the corresponding reagent tube placing tube 13, and compressing the third spring 40;
the electric telescopic rod 19 drives the first supporting rod 16 and the second supporting rod 17 to rapidly reciprocate to lift, so that the connecting rod 18 lifts in the second accommodating groove 15, the connecting block 22 is further driven to lift in the first accommodating groove 14, the arc-shaped clamping plate 25 lifts in the reagent tube placing cylinder 13, and finally the reagent tube 26 is driven to lift, so that a sample in the reagent tube 26 is vibrated, the sample solution and the cell preservation solution are uniformly mixed, the stretching speed of the electric telescopic rod 19 is slowed down after uniform shaking, and the mixed solution in the reagent tube 26 slightly vibrates;
conveying water in a cold water storage tank 5 to the inside of a water tank 10, monitoring the internal temperature of a reagent tube placing cylinder 13 in real time by a temperature sensor 43, cooling the water in the water tank 10 by a refrigerator 11, so that the internal temperature of the reagent tube placing cylinder 13 reaches the optimal temperature suitable for storing a gene sample stored in a reagent tube 26, and the mixed liquid in the reagent tube 26 is always in a slight oscillation state, so that the freezing is uniform, crystals are prevented from being separated out, the sample in the reagent tube 26 can be stored for a long time, and the transportation is convenient;
when the sample in the reagent tube 26 needs to be restored to the room temperature for detection, ice water in the water tank 10 is conveyed into the cold water storage tank 5, normal-temperature water in the hot water storage tank 6 is conveyed into the water tank 10, the temperature is raised through the heater 12, the temperature inside the reagent tube placing cylinder 13 is always at the room temperature, the mixed liquid inside the reagent tube 26 is always in a slight oscillation state, the heating is uniform, the local heating is avoided, the cell activity is damaged, and the sample in the reagent tube 26 can be thawed within a short time;
after the sample in the reagent tube 26 is thawed, the electric telescopic rod 19 stops moving, the box cover 27 is opened, the box cover 27 is closed after the reagent tube 26 is completely taken out, when the movable sleeve 32 is inserted into the second positioning hole 37, the circular pressing plate 41 is far away from the reagent tube placing barrel 13, the ultraviolet lamp 42 is started to sterilize the inside of the reagent tube placing barrel 13 and the surface of the sealing plate 20, after the sterilization is completed, the ultraviolet lamp 42 is closed, hot water in the water tank 10 is conveyed into the hot water storage tank 6, ice water in the cold water storage tank 5 is conveyed back into the water tank 10 again, and the device can be used again for storing gene samples.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a gene detection uses reagent pipe storage device, includes box (1), its characterized in that: the surface of both sides of the box body (1) is provided with heat dissipation holes (2) in a penetrating manner, the two sides inside the box body (1) are respectively provided with a support plate (3), the bottom surface inside the box body (1) is provided with a controller (4), a cold water storage tank (5) and a hot water storage tank (6), the upper ends of the cold water storage tank (5) and the hot water storage tank (6) are provided with a water tank (10), the upper end inside the water tank (10) is provided with a first holding tank (14) and a second holding tank (15), the intersection of the first holding tank (14) and the second holding tank (15) is communicated, the first holding tank (14) respectively penetrates through the two sides of the upper end of the reagent tube placing cylinder (13), the second holding tank (15) is respectively arranged between two adjacent reagent tube placing cylinders (13), and the two ends of the first holding tank (14) are respectively provided with a first support rod (16), the two ends of the second accommodating groove (15) are respectively provided with a second supporting rod (17), the first supporting rod (16) and the second supporting rod (17) are connected to form a closed rectangle, the first supporting rod (16) and the second supporting rod (17) are arranged in an area formed by the inner wall of the box body (1) and the outer wall of the water tank (10), a connecting rod (18) is fixedly connected between the two second supporting rods (17), the connecting rod (18) is arranged in the second accommodating groove (15), an electric telescopic rod (19) is arranged on the lower surface of the joint of the first supporting rod (16) and the second supporting rod (17), the electric telescopic rod (19) is fixedly arranged on the two sides of the upper end face of the supporting plate (3), a sealing plate (20) is fixedly arranged on the upper end of the water tank (10), and a round hole (21) penetrates through the surface of the sealing plate (20), round hole (21) with the position, the size of a section of thick bamboo (13) are placed to the reagent pipe are corresponding, connecting rod (18) both sides with the equal fixedly connected with connecting block (22) in one side that first bracing piece (16) are close to each other, connecting block (22) set up inside first holding tank (14), connecting block (22) end difference fixedly connected with connecting plate (23).
2. The storage device for a reagent vessel for gene testing according to claim 1, wherein: the cold water storage tank (5) and the hot water storage tank (6) are respectively arranged at one side where the two supporting plates (3) are close to each other, the controller (4) is arranged at a region between the cold water storage tank (5) and the hot water storage tank (6), one side where the cold water storage tank (5) and the hot water storage tank (6) are close to each other is provided with a water inlet pipe (7) and a water outlet pipe (8), the water inlet side of the water inlet pipe (7) and the water outlet side of the water outlet pipe (8) are respectively provided with a water pump (9), the water inlet pipe (7) and the water outlet pipe (8) are both communicated with the inside of the water tank (10), the bottom surface inside the water tank (10) is provided with a refrigerator (11) and a heater (12), the upper end inside the water tank (10) is provided with a reagent pipe placing cylinder (13), and the reagent pipes placing cylinders (13) are uniformly distributed, the bottom surface of the outside of the reagent tube placing cylinder (13) is not in contact with the refrigerator (11) and the heater (12), and the upper ends of the first accommodating groove (14) and the second accommodating groove (15) are open and the lower ends of the first accommodating groove and the second accommodating groove are closed.
3. The storage device for a reagent vessel for gene assaying according to claim 2, characterized in that: the connecting plate (23) is respectively arranged in the reagent tube placing barrel (13), is located at the same position in the reagent tube placing barrel (13), is provided with two springs (24) at the lower end of one side, close to each other, of the connecting plate (23), is sleeved with a telescopic guide rod inside the first spring (24), and the tail end of the first spring (24) is respectively and fixedly provided with an arc-shaped clamping plate (25).
4. The storage device for a reagent vessel for gene assaying according to claim 3, characterized in that: arc grip block (25) medial surface is provided with the protection cushion respectively, is located same the reagent pipe is placed a section of thick bamboo (13) inside two be provided with reagent pipe (26) between arc grip block (25), reagent pipe (26) are including pipe shaft (261) and tube cap (262), tube cap (262) set up pipe shaft (261) upper end, tube cap (262) lower surface with arc grip block (25) up end parallel and level.
5. The storage device for a reagent vessel for gene assaying according to claim 4, characterized in that: box (1) upper end has case lid (27) through hinge movable mounting, box (1) with through buckle (28) fixed connection between case lid (27), case lid (27) external surface is provided with control panel (29), control panel (29) both sides are provided with fixed block (30) respectively, fixed block (30) end fixed mounting respectively has second spring (31), second spring (31) outside is provided with movable sleeve (32), the outer wall of fixed block (30) with the inner wall size phase-match of movable sleeve (32).
6. The storage device for a reagent vessel for gene assaying according to claim 5, characterized in that: second spring (31) with the inner wall fixed connection of activity sleeve (32), activity sleeve (32) are close to the one end upper surface of control panel (29) is provided with arm-tie (33), fixed block (30) are kept away from one side of control panel (29) is provided with mounting hole (34), mounting hole (34) set up case lid (27) surface both sides, mounting hole (34) inside is run through respectively and is provided with spliced pole (35), spliced pole (35) side is run through respectively and is provided with first locating hole (36) and second locating hole (37).
7. The storage device for a reagent vessel for gene assaying according to claim 6, characterized in that: first locating hole (36) with second locating hole (37) all with the outer wall size phase-match of activity sleeve (32), second locating hole (37) set up first locating hole (36) lower extreme, spliced pole (35) upper end fixedly connected with anti-skidding handle (38), spliced pole (35) lower extreme fixed mounting has mounting panel (39), mounting panel (39) set up inside case lid (27), mounting panel (39) fixed surface installs third spring (40), third spring (40) end is provided with circular pressing plate (41) respectively.
8. The storage device for a reagent vessel for gene assaying according to claim 7, characterized in that: mounting panel (39) fixed surface installs ultraviolet lamp (42), one of them reagent pipe is placed a section of thick bamboo (13) inner wall surface and is provided with temperature sensor (43), water pump (9), refrigerator (11), heater (12), electric telescopic handle (19), ultraviolet lamp (42), temperature sensor (43) all with controller (4) with control panel (29) are through electric signal connection.
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