CN113337399A

CN113337399A - Plant cell culture device of daylighting floats

Info

Publication number: CN113337399A
Application number: CN202110455277.3A
Authority: CN
Inventors: 张庆兰
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-04-26
Filing date: 2021-04-26
Publication date: 2021-09-03

Abstract

The invention discloses a floating lighting plant cell culture device, belonging to the technical field of cell culture, and the floating lighting plant cell culture device comprises a mounting frame, wherein a plurality of uniformly distributed sleeves are fixedly connected on the mounting frame, a floating barrel is inserted in the sleeves, a plurality of sliding holes matched with the floating barrel are drilled on the mounting frame, a culture barrel is placed in the floating barrel, the upper end of the floating barrel is clamped with a sealing plug, the upper end and the lower end of the outer wall of the floating barrel are fixedly connected with snap rings, the top end of the sleeve is clamped with a sealing plug matched with the floating barrel, the sealing plug comprises a sealing cover main body, an elastic press ring is fixedly connected in the sealing cover main body, the top end of the sealing cover main body is fixedly connected with a mesh cover, the floating or sinking of the culture device can be controlled by the amount of carbon dioxide released by the cultivated plants in a dark environment, and the dark culture and the bright culture can be automatically adjusted according to the growth conditions of plant cells.

Description

Plant cell culture device of daylighting floats

Technical Field

The invention relates to the technical field of cell culture, in particular to a floating lighting plant cell culture device.

Background

A dedifferentiation culture stage: since light can obstruct the dedifferentiation of the tissue, the dedifferentiation culture stage should be cultured in the dark, and the callus can grow faster in the absence of light. The dedifferentiation culture period is typically 2 weeks, and then the medium must be changed for culture, since the nutrients in the medium are nearly exhausted after 2 weeks.

Callus formation requires light-resistant culture. The reason why callus formation becomes slow in the presence of light: plant cells spontaneously undergo photosynthesis under light conditions, which is slow because repair is accomplished. The result of photosynthesis is the production of sugars, but a proportion of the material and energy must be diverted during the process, thereby slowing the overall quantity of material and energy required to complete the remediation process.

In general, when callus is formed, if light is present, cell redifferentiation is likely to occur, and the purpose of forming a large amount of callus and obtaining a large number of seedlings in a short period of time cannot be achieved.

At present, the time control of irrelevant culture and light culture is difficult to control in most of artificially cultured plant cells, so that the dedifferentiation growth state of plants is influenced.

Disclosure of Invention

1. Technical problem to be solved

The invention aims to provide a floating lighting plant cell culture device, which can realize the control of the floating or sinking of the culture device through the amount of carbon dioxide released by the cultured plant in a non-light environment, realize the automatic adjustment of non-light culture and light culture according to the growth condition of plant cells and ensure that technicians can control the dedifferentiation of the plant cells according to the needs.

2. Technical scheme

In order to solve the above problems, the present invention adopts the following technical solutions.

A plant cell culture device capable of realizing floating lighting comprises a mounting frame, wherein a plurality of sleeves which are uniformly distributed are fixedly connected to the mounting frame, a liquid injection tank is clamped at the top ends of the sleeves, a floating barrel is inserted in the sleeves, a plurality of sliding holes matched with the floating barrel are drilled in the mounting frame, a culture barrel is placed in the floating barrel, a sealing plug is clamped at the upper end of the floating barrel, snap rings are fixedly connected to the upper end and the lower end of the outer wall of the floating barrel, the sealing plug matched with the floating barrel is clamped at the top end of the sleeve and comprises a sealing cover main body, an elastic compression ring is fixedly connected in the sealing cover main body, a mesh cover is fixedly connected at the top end of the sealing cover main body, a plurality of liquid injection air guide pipes connected with the elastic compression ring are installed at the lower end of the sealing cover main body, a liquid injection pipe is fixedly connected to the bottom end of the sealing cover main body, and the mesh cover is communicated with the mesh cover, fixedly connected with and the unsteady push pedal of a section of thick bamboo assorted that floats on the telescopic inner wall, the push pedal that floats includes fixed plate and ripple ring, be connected with the fly leaf between fixed plate and the ripple ring, install the gas tube on the fixed plate, the downside of mounting bracket is equipped with the mount, be connected with two pairs of backup pads between mounting bracket and the mount, adjacent two be connected with the observation board between the backup pad, can realize controlling culture apparatus come-up or sink through the carbon dioxide volume of the plant of cultivating release under no light environment, realize carrying out automatically regulated dull culture and have the light to cultivate according to the plant cell growth condition, make technical staff can control the plant cell as required and take off the differentiation.

Furthermore, install in the slide opening of mounting bracket with the electric heat ring of unsteady section of thick bamboo assorted, the bottom fixed connection electromagnetism magnet ring of mounting bracket, fixedly connected with and the work of electromagnetism magnet ring assorted becket on the snap ring of unsteady section of thick bamboo lower extreme make unsteady section of thick bamboo and electromagnet attract, realize that the unsteady section of thick bamboo is in the sleeve all the time, guarantee that plant cell carries out dull culture for a long time.

Furthermore, a plurality of evenly distributed's fluorescent lamp plate is installed on the top of mount, and after a section of thick bamboo sinks, the fluorescent lamp plate work floats a section of thick bamboo and carries out the cultivation of having light to the interior plant cell of messenger.

Further, annotate the liquid jar including the apron, the bottom fixedly connected with bin of apron, install the flow regulating valve in the bin, the bottom fixedly connected with of bin drips irrigation the pipe with flow regulating valve assorted, drip irrigation the position phase-match of pipe and elastic compression ring, make things convenient for in the nutrient solution pours into a floating section of thick bamboo.

Furthermore, the sleeve is made of light-tight materials, the whole floating cylinder is made of temperature-variable glass, after the floating cylinder sinks, the floating cylinder is heated by controlling the electric heating ring in the mounting frame to change the light transmittance of the floating cylinder, and therefore the technical staff can adjust the floating cylinder manually to culture in any position without light and in any position with light.

Furthermore, a heat insulation film is laid between the inner walls of the supporting plate and the observation plate, and a reflective film is laid on the heat insulation film.

Furthermore, a permeable membrane is mounted on the mesh cover, a plurality of water permeable holes are uniformly distributed on the permeable membrane, the aperture of each water permeable hole is 100-200nm, the gap between the permeable membrane and the mesh cover is 1-2mm, and the permeable membrane is made of flexible resin.

Further, install the block terminal in the mount and with annotate liquid jar assorted air pump, in the air pump with annotate and be connected with the air duct between the liquid jar, the laminating of ware air duct and backup pad and run through the mounting bracket, install the controller in the block terminal, the fluorescent lamp plate, annotate liquid jar, electric heat ring and electro-magnet ring all with controller signal connection.

Further, the elasticity clamping ring includes the elasticity silica gel ring, the elasticity silica gel intra-annular is filled with the inflation sponge, the inflation sponge intussuseption is filled with the oxygen adsorbent, adsorbs except that the gas of carbon dioxide through the inflation sponge in the elasticity clamping ring, and elasticity clamping ring inflation when making the carbon dioxide excessive, and then the extrusion is annotated the liquid pipe, makes and annotates the liquid pipe and block up.

A floating lighting plant cell culture device comprises the following use methods:

s1, a technician selects required plant cells to place in the observation plate, then clamps the observation plate into the floating barrel, clamps the sealing plug to seal, controls the air pump to inflate the liquid injection tank, enables the liquid injection tank to expand and supports the floating barrel upwards until the bottom end of the floating barrel is attached to the bottom end of the mounting frame;

s2, after the nutrient with proper dosage is stored in the liquid injection tank, the liquid injection tank is clamped on the sleeve, and drip irrigation is carried out on the sealing plug through the flow regulating valve;

s3, performing dull culture on the plants in the floating barrel in the mounting frame, and injecting the nutrient discharged by the liquid injection tank into the floating barrel through the sealing plug;

and S4, when the carbon dioxide discharged by the plant cells in the observation plate is excessive, the elastic pressure ring in the sealing plug absorbs the carbon dioxide and expands, so that the liquid injection pipe is squeezed to be blocked, the nutrient is accumulated in the sealing cover main body, and the liquid injection pipe is recovered until the carbon dioxide is consumed under the photosynthesis of the plant.

3. Advantageous effects

Compared with the prior art, the invention has the advantages that:

(1) the scheme can realize that the floating or sinking of the culture device is controlled by the amount of carbon dioxide released by the cultivated plant in a dark environment, and realize automatic adjustment of dark culture and bright culture according to the growth condition of plant cells, so that technicians can control the dedifferentiation of the plant cells according to needs.

(2) The sliding hole of the mounting frame is internally provided with an electric heating ring matched with the floating cylinder, the bottom end of the mounting frame is fixedly connected with an electromagnet ring, and a clamping ring at the lower end of the floating cylinder is fixedly connected with a metal ring matched with the electromagnet ring to control the electromagnet ring to work, so that the floating cylinder is attracted with the electromagnet, the floating cylinder is always positioned in the sleeve, and the plant cells are ensured to be cultured in a dark mode for a long time.

(3) Annotate the liquid jar and include the apron, the bottom fixedly connected with bin of apron installs the flow regulating valve in the bin, and the bottom fixedly connected with of bin drips irrigation the pipe with flow regulating valve assorted, drips irrigation the position phase-match of pipe and elastic compression ring, makes things convenient for in the nutrient solution pours into a floating section of thick bamboo. .

(4) The sleeve is made of light-tight materials, the whole floating cylinder is made of temperature-variable glass, after the floating cylinder sinks, the floating cylinder is heated by controlling the electric heating ring in the mounting frame to change the light transmittance of the floating cylinder, and therefore technical staff can adjust the floating cylinder manually to culture in a non-light mode and in a light mode at any position.

(5) The elasticity clamping ring includes the elasticity silica gel ring, and the elasticity silica gel intra-annular is filled with the inflation sponge, and the inflation sponge intussuseption is filled with the oxygen adsorbent, adsorbs except that the gas of carbon dioxide through the inflation sponge in the elasticity clamping ring, and elasticity clamping ring inflation when making the carbon dioxide excessive, and then the liquid pipe is annotated in the extrusion, makes annotate the liquid pipe and blocks up.

Drawings

FIG. 1 is a perspective view of the present invention;

FIG. 2 is a cross-sectional view of the present invention;

FIG. 3 is a schematic view of the structure at A in FIG. 2;

FIG. 4 is a structural diagram of the floating state of the floating cylinder according to the present invention;

fig. 5 is a front view of the present invention.

The reference numbers in the figures illustrate:

the device comprises a mounting rack 1, a sleeve 2, a floating barrel 3, a fixing rack 4, a supporting plate 5, an observation plate 6, a liquid injection tank 7, a cover plate 701, a storage tank 702, a flow regulating valve 703, a sealing plug 8, a sealing cover 801 main body, an elastic compression ring 802, a mesh cover 803, an air guide pipe 804, a liquid injection pipe 805, a floating push plate 9, a fixing plate 901, a corrugated ring 902 and a daylight lamp panel 10.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the connection can be direct connection or indirect connection through an intermediate medium, and can be communication inside the model adapting element. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example 1:

referring to fig. 1-5, a floating lighting plant cell culture device comprises an installation frame 1, a plurality of uniformly distributed sleeves 2 are fixedly connected to the installation frame 1, a liquid injection tank 7 is clamped at the top ends of the sleeves 2, the liquid injection tank 7 comprises a cover plate 701, a storage tank 702 is fixedly connected to the bottom end of the cover plate 701, nutrient solution is stored in the storage tank 702, a flow regulating valve 703 is installed in the storage tank 702, a drip irrigation pipe matched with the flow regulating valve 703 is fixedly connected to the bottom end of the storage tank 702, the position of the drip irrigation pipe is matched with that of an elastic pressing ring 802, the nutrient solution is conveniently poured into a floating cylinder 3, a floating cylinder 3 is inserted into the sleeve 2, a plurality of sliding holes matched with the floating cylinder 3 are drilled on the installation frame 1, a culture cylinder is placed in the floating cylinder 3, the upper end of the floating cylinder 3 is clamped 8, clamping rings are fixedly connected to the upper and lower ends of the outer wall of the floating, the sleeve 2 is made of light-tight materials, the floating cylinder 3 is integrally made of temperature-change glass, after the floating cylinder 3 sinks, the floating cylinder 3 is heated by controlling an electric heating ring in the mounting frame 1, so that the light transmittance of the floating cylinder 3 is changed, and the lightless culture of the floating cylinder 3 at any position can be manually adjusted by technicians.

Referring to fig. 1-3, the sealing plug 8 includes a sealing cap body 801, an elastic press ring 802 is fixedly connected in the sealing cap body 801, a mesh cover 803 is fixedly connected at the top end of the sealing cap body 801, a plurality of air ducts 804 connected with the elastic press ring 802 are installed at the lower end of the sealing cap body 801, an injection pipe 805 is fixedly connected at the bottom end of the sealing cap body 801, the injection pipe 805 is located in the sealing cap body 801 and made of an elastic adhesive film, the part matched with the elastic press ring 802 can be pressed and blocked when the elastic press ring 802 expands inwards, the injection pipe 805 is communicated with the mesh cover 803, and a floating push plate 9 matched with the floating barrel 3 is fixedly connected on the inner wall of the sleeve 2;

referring to fig. 3, the floating push plate 9 includes a fixed plate 901 and a corrugated ring 902, a movable plate 903 is connected between the fixed plate 901 and the corrugated ring 902, an air tube is installed on the fixed plate 901, a fixed frame 4 is installed on the lower side of the installation frame 1, a plurality of uniformly distributed fluorescent lamp panels 10 are installed on the top end of the fixed frame 4, and after the floating cylinder 3 sinks, the fluorescent lamp panels 10 work to allow plant cells in the floating cylinder 3 to be cultured with light.

The mesh cover 803 is provided with a permeable membrane, a plurality of uniformly distributed permeable holes are formed in the permeable membrane, the aperture of each permeable hole is 100-200nm, the gap between the permeable membrane and the mesh cover 803 is 1-2mm, and the permeable membrane is made of flexible resin. Elastic pressure ring 802 includes the elastic silica gel ring, and the elastic silica gel ring intussuseption is filled with the inflation sponge, and the inflation sponge intussuseption is filled with the oxygen adsorbent, adsorbs the gas except carbon dioxide through the inflation sponge in elastic pressure ring 802, and elastic pressure ring 802 inflation when making carbon dioxide excessive, and then extrudees notes liquid pipe 805, makes notes liquid pipe 805 block up.

Referring to fig. 1-4, two pairs of supporting plates 5 are connected between the mounting frame 1 and the fixing frame 4, an observation plate 6 is connected between two adjacent supporting plates 5, a heat insulation film is laid between the supporting plates 5 and the inner wall of the observation plate 6, a reflective film is laid on the heat insulation film, an electric heating ring matched with the floating cylinder 3 is installed in a sliding hole of the mounting frame 1, an electric magnet ring is fixedly connected to the bottom end of the mounting frame 1, a metal ring matched with the electric magnet ring is fixedly connected to a snap ring at the lower end of the floating cylinder 3, the metal ring is controlled to work by controlling the electric magnet ring, so that the floating cylinder 3 is attracted with the electric magnet, the floating cylinder 3 is always positioned in the sleeve 2, plant cells are ensured to be cultured for a long time without light, a distribution box and an air pump matched with the liquid injection tank 7 are installed in the fixing frame 4, an air duct is connected between the air pump and the liquid injection tank 7, the air duct is attached to the supporting plates 5 and penetrates through the mounting frame 1, the controller is installed in the distribution box, and the fluorescent lamp panel 10, the liquid injection tank 7, the electric heating ring and the electromagnet ring are all in signal connection with the controller.

s1, a technician selects required plant cells to place in an observation plate 6, then the observation plate 6 is clamped in a floating cylinder 3, a sealing plug 8 is clamped for sealing, an air pump is controlled to inflate the liquid injection tank 7, so that a floating push plate 9 expands and supports the floating cylinder 3 upwards until the bottom end of the floating cylinder 3 is attached to the bottom end of an installation frame 1, the thrust provided by the floating push plate 9 is equal to the gravity borne by the floating cylinder 3 at the moment, and the floating cylinder 3 is in a stress balance state;

s2, after a nutrient with proper dosage is stored in the liquid injection tank 7, the liquid injection tank 7 is clamped on the sleeve 2, and drip irrigation is carried out on the sealing plug 8 through the flow regulating valve 703;

s3, performing dull culture on the plants in the floating cylinder 3 in the mounting frame 1, and injecting the nutrient discharged from the liquid injection tank 7 into the floating cylinder 3 through the sealing plug 8;

s4, when the carbon dioxide discharged by the plant cells in the observation plate 6 is excessive, the elastic pressure ring 802 in the sealing plug 8 absorbs the carbon dioxide and then expands, so that the part, matched with the position of the elastic pressure ring 802, of the liquid injection pipe 805 is extruded and blocked, so that the nutrient is accumulated in the sealing cover main body 801, the whole weight of the floating cylinder 3 is increased, the gravity borne by the floating cylinder 3 is greater than the supporting force provided by the floating push plate 9, the floating cylinder 3 presses the floating push plate 9 downwards, the floating push plate 9 sinks downwards, after the carbon dioxide is consumed under the photosynthesis of the plant, the elastic pressure ring 802 and the liquid injection pipe 805 recover under the content of the carbon dioxide, the nutrient solution is consumed by the plant cells, the accumulated nutrient solution is consumed, the whole weight of the floating cylinder 3 is reduced, and the floating cylinder 3 gradually floats upwards again at the moment.

The scheme can realize that the floating or sinking of the culture device is controlled by the amount of carbon dioxide released by the cultivated plant in a dark environment, and realize automatic adjustment of dark culture and bright culture according to the growth condition of plant cells, so that technicians can control the dedifferentiation of the plant cells according to needs.

The foregoing is only a preferred embodiment of the present invention; the scope of the invention is not limited thereto. Any person skilled in the art should be able to cover the technical scope of the present invention by equivalent or modified solutions and modifications within the technical scope of the present invention.

Claims

1. The utility model provides a plant cell culture device of daylighting floats, includes mounting bracket (1), its characterized in that: the sealing device is characterized in that a plurality of uniformly distributed sleeves (2) are fixedly connected to the mounting rack (1), a liquid injection tank (7) is clamped at the top ends of the sleeves (2), floating barrels (3) are inserted in the sleeves (2), a plurality of sliding holes matched with the floating barrels (3) are drilled on the mounting rack (1), culture barrels are placed in the floating barrels (3), sealing plugs (8) are clamped at the upper ends of the floating barrels (3), snap rings are fixedly connected to the upper end and the lower end of the outer wall of the floating barrel (3), the sealing plugs (8) matched with the floating barrels (3) are clamped at the top ends of the sleeves (2), each sealing plug (8) comprises a sealing cover main body (801), an elastic pressure ring (802) is fixedly connected to the inside of the sealing cover main body (801), a mesh cover (803) is fixedly connected to the top end of the sealing cover main body (801), a plurality of air guide tubes (804) connected with the elastic pressure ring (802) are installed at the lower end of the sealing cover main body (801), the bottom end of the sealing cover main body (801) is fixedly connected with a liquid injection pipe (805), the liquid injection pipe (805) is communicated with the mesh cover (803), the inner wall of the sleeve (2) is fixedly connected with a floating push plate (9) matched with the floating barrel (3), the floating push plate (9) comprises a fixed plate (901) and a corrugated ring (902), a movable plate (903) is connected between the fixed plate (901) and the corrugated ring (902), an inflation pipe is installed on the fixed plate (901), a fixing frame (4) is arranged on the lower side of the mounting frame (1), two pairs of supporting plates (5) are connected between the mounting frame (1) and the fixing frame (4), and an observation plate (6) is connected between the adjacent two supporting plates (5).

2. A floating light plant cell culture apparatus according to claim 1, wherein: the electric heating ring matched with the floating barrel (3) is installed in the sliding hole of the mounting rack (1), the bottom end of the mounting rack (1) is fixedly connected with an electromagnetic ring, and a metal ring matched with the electromagnetic ring is fixedly connected to a clamping ring at the lower end of the floating barrel (3).

3. A floating light plant cell culture apparatus according to claim 1, wherein: a plurality of uniformly distributed fluorescent lamp panels (10) are installed on the top end of the fixing frame (4).

4. A floating light plant cell culture apparatus according to claim 1, wherein: annotate fluid reservoir (7) including apron (701), the bottom fixedly connected with storage box (702) of apron (701), install in storage box (702) and adjust the flow valve (703), the bottom fixedly connected with of storage box (702) drips irrigation the pipe with adjusting flow valve (703) assorted, drip irrigation the position phase-match of pipe and elasticity clamping ring (802).

5. A floating light plant cell culture apparatus according to claim 1, wherein: the sleeve (2) is made of opaque materials, and the whole floating cylinder (3) is made of temperature-variable glass.

6. A floating light plant cell culture apparatus according to claim 1, wherein: and a heat insulation film is paved between the inner walls of the supporting plate (5) and the observation plate (6), and a reflective film is paved on the heat insulation film.

7. A floating light plant cell culture apparatus according to claim 1, wherein: the mesh cover (803) is provided with a permeation membrane, the permeation membrane is provided with a plurality of water permeable holes which are uniformly distributed, the aperture of each water permeable hole is 100-200nm, the gap between the permeation membrane and the mesh cover (803) is 1-2mm, and the permeation membrane is made of flexible resin.

8. A floating light plant cell culture apparatus according to claim 1, wherein: install the block terminal in mount (4) and with annotate fluid reservoir (7) assorted air pump, be connected with the air duct in the air pump between fluid reservoir (7), the laminating of ware air duct and backup pad (5) runs through mounting bracket (1), install the controller in the block terminal, fluorescent lamp plate (10), annotate fluid reservoir (7), electric heat ring and electromagnet ring all with controller signal connection.

9. A floating light plant cell culture apparatus according to claim 1, wherein: the elastic pressure ring (802) comprises an elastic silica gel ring, an expansion sponge is filled in the elastic silica gel ring, and an oxygen adsorbent is filled in the expansion sponge.

10. The floating lighting plant cell culture device according to claim 1, wherein the use method comprises:

s1, a technician selects required plant cells to place in an observation plate (6), then clamps the observation plate (6) into a floating cylinder (3), clamps a sealing plug (8) for sealing, controls an air pump to inflate the liquid injection tank (7), expands a floating push plate (9) and supports the floating cylinder (3) upwards until the bottom end of the floating cylinder (3) is attached to the bottom end of an installation frame (1);

s2, after a nutrient with proper dosage is stored in the liquid injection tank (7), the liquid injection tank (7) is clamped on the sleeve (2), and drip irrigation is carried out on the sealing plug (8) through the flow regulating valve (703);

s3, performing light-free culture on plants in the floating cylinder (3) in the mounting rack (1), and injecting a nutrient discharged from the liquid injection tank (7) into the floating cylinder (3) through the sealing plug (8);

s4, when the carbon dioxide discharged from the plant cells in the observation plate (6) is excessive, the elastic press ring (802) in the sealing plug (8) absorbs the carbon dioxide and expands, and the liquid injection tube (805) is pressed and blocked, so that the nutrient is accumulated in the sealing cap body (801), and the liquid injection tube (805) is restored after the carbon dioxide is consumed by the photosynthesis of the plant.