CN113332752A

CN113332752A - Continuous flow chromatography assembly, system and method

Info

Publication number: CN113332752A
Application number: CN202110525790.5A
Authority: CN
Inventors: 王洪春; 栗云天
Original assignee: Beijing Huachuang Jingke Biotechnology Co ltd
Current assignee: Zhejiang JYSS Bio Engineering Co Ltd
Priority date: 2021-05-14
Filing date: 2021-05-14
Publication date: 2021-09-03

Abstract

The invention discloses a continuous flow chromatography component, a system and a method, comprising a rotor and a stator, wherein the rotor rotates on the stator, the rotor is provided with a switching pointer groove, a bypass pointer groove, a switching groove and a bypass groove, the stator is provided with a sample port, a chromatography switching port, a jumper switching port and a cleaning port, in the process of rotating the rotor, one end of the bypass pointer groove is communicated with the bypass groove, the other end of the bypass pointer groove is communicated with different jumper switching ports in a switching way, one end of the switching pointer groove is communicated with the sample port, the other end of the switching pointer groove is communicated with different chromatography switching ports in a switching way, one end of the switching groove is communicated with different chromatography switching ports in a switching way, and the other end of the switching groove is communicated with different jumper switching ports in a switching way; the invention realizes a plurality of switching chromatographs, greatly improves the chromatographic efficiency, reduces the number of continuous flow chromatographic components, simplifies the flow path, facilitates the control and reduces the failure rate.

Description

Continuous flow chromatography assembly, system and method

Technical Field

The invention relates to the technical field of biological chromatography, in particular to a continuous flow chromatography component, a system and a method.

Background

The traditional chromatography technology adopts a batch processing method, adopts a single chromatographic column, and has low efficiency when samples are balanced, loaded, eluted, washed and the like in batches. The continuous flow chromatography technology is used as the next generation of chromatography technology direction, a plurality of chromatographic columns are adopted, samples are continuously loaded, the columns which are saturated in adsorption are switched out of a series column sequence under the condition that the sample loading is not stopped according to the adsorption saturation condition of each column in the series columns, the steps of elution, CIP (cleaning in place) and the like are independently carried out, the columns are added to the end of the series column sequence again after the completion of the elution, and the continuous loading and the multi-batch elution are realized through the alternation.

In continuous flow chromatography, 2-8 chromatographic columns are generally connected in series, so that the chromatography efficiency is greatly improved, chromatographic columns with smaller sizes and fillers with smaller volumes can be used, the same sample treatment capacity is achieved, or the production capacity is greatly improved under the conditions of the same filler volume and the size of the chromatographic columns, the buffer solution consumption is reduced, the equipment size and the occupied area are reduced, and the energy conservation and emission reduction of a factory building are facilitated.

At present, continuous flow chromatography equipment with 2 columns, 3 columns, 4 columns and 6 columns is available on the market, and only 2 columns, 3 columns, 4 columns or 6 columns can be used in the continuous flow chromatography process, the number of the chromatography columns cannot be freely switched, and the system is very complex.

The existing laboratory continuous flow chromatography system has numerous switching components, complex pipeline connection and complex control, takes three-column continuous flow as an example, at least 6 pointer type inlet switching components and 6 outlet switching components are needed, and a plurality of tee joints are additionally arranged to realize the random switching between 3 columns and 2 columns; if the number of columns is increased, the number of inlet/outlet switching modules and the number of tees are also increased accordingly, and the number of connectable columns is fixed, so that the number of chromatography columns cannot be increased or decreased as required.

Disclosure of Invention

The invention aims to solve the problems of very complex continuous flow chromatography equipment system, numerous continuous flow chromatography components, complex connection and complex control in the prior art, and provides a continuous flow chromatography component, a continuous flow chromatography system and a continuous flow chromatography method capable of working continuously.

In order to achieve the purpose, the invention adopts the following technical scheme:

a continuous flow chromatography component comprises a rotor and a stator, wherein the rotor rotates on the stator, the rotor is provided with a switching pointer groove, a bypass pointer groove, a switching groove and a bypass groove, the stator is provided with a sample port, a chromatography switching port, a jumper switching port and a cleaning port, in the rotating process of the rotor, one end of the bypass pointer groove is communicated with the bypass groove, the other end of the bypass pointer groove is communicated with different jumper switching ports in a switching mode, one end of the switching pointer groove is communicated with the sample port, the other end of the switching pointer groove is communicated with different chromatography switching ports in a switching mode, one end of the switching groove is communicated with different chromatography switching ports in a switching mode, and the other end of the switching groove is communicated with different jumper switching ports in a switching mode.

Preferably, the switching pointer groove, the bypass pointer groove and the switching groove are arranged on the rotor in a non-communicated mode, the bypass pointer groove and the bypass groove are arranged on the rotor in a communicated mode, two ends of the switching pointer groove are respectively communicated with the sample port and the chromatography switching port, two ends of the bypass pointer groove are respectively communicated with the jumper switching port and the bypass groove, two ends of the switching groove are respectively communicated with the chromatography switching port and the jumper switching port, and the bypass groove is communicated with the bypass pointer groove and the cleaning port.

Further, switch pointer groove, bypass pointer groove along rotor radial extension, switch the groove or for the arc wall or for straight line type groove or for slash type groove along rotor circumference extension, switch the adjacent setting in pointer groove and bypass pointer groove, switch the groove and distribute along rotor circumference and set up, the chromatography switches mouthful all along the rotation direction circumference with the wire jumper and switches mouthful and distribute, the chromatography switches mouthful and the wire jumper and switches mouthful the setting in turn of the same quantity, sample mouth and washing mouth all with the chromatography switch mouthful and the wire jumper switch mouthful distribution circumference noncoincidence.

Furthermore, the distribution circumference of the chromatography switching port and the distribution circumference of the jumper switching port are equal in diameter, the sample port is located in the circle center of the distribution circumference, the cleaning port is located outside the distribution circumference, the bypass groove is closed to surround the periphery of the rotor, or the cleaning port is located in the distribution circumference, and the bypass groove is not closed to surround the center of the rotor.

The invention also provides a continuous flow chromatography system, which also comprises M jumper channels, N chromatography channels, a sample channel, a cleaning liquid channel, a chromatography column, a sample loading pump and a cleaning pump, wherein the number of the continuous flow chromatography components is two, M is more than or equal to 1, N is more than or equal to 1, and the sequential cyclic switching of the M jumper channels and the N chromatography channels is realized through the clockwise or anticlockwise sequential rotation of rotors of the two continuous flow chromatography components.

Furthermore, the two ends of each chromatographic channel are respectively connected with the chromatographic switching ports of the two switching assemblies, each chromatographic switching port of a single switching assembly is correspondingly connected with one chromatographic channel, a chromatographic column is arranged in each chromatographic channel, the two ends of each jumper channel are respectively and sequentially connected with the jumper switching ports of the two switching assemblies, each jumper switching port of a single switching assembly is correspondingly connected with one jumper channel, the sample channels are communicated with the sample ports, the cleaning liquid channels are communicated with the cleaning ports, sample loading pumps are arranged in the sample channels, and cleaning liquid channels are provided with cleaning pumps.

Preferably, the number of the chromatographic columns is N, N is 2 or 3 or 4 or 6, the number of the chromatographic switching ports and the number of the jumper switching ports of the switching assembly are the same as that of the chromatographic columns, the number M of the jumper channels is the same as that of the chromatographic channels, the angles of the chromatographic switching ports and the jumper switching ports are 360 degrees/N uniformly distributed along the circumference, and the number of the switching grooves of the switching assembly is N-1.

Further, the continuous flow chromatography system also comprises a first chromatography column, a second chromatography column, a third chromatography column, a continuous flow chromatography component A and a continuous flow chromatography component B, wherein a rotor A of the continuous flow chromatography component A is provided with a switching pointer groove A, a bypass pointer groove A, a first switching groove A, a second switching groove A and a bypass groove A, a rotor B of the continuous flow chromatography component B is provided with a switching pointer groove B, a bypass pointer groove B, a first switching groove B, a second switching groove B and a bypass groove B, a stator of the continuous flow chromatography component A is provided with a chromatography switching port 1A, a chromatography switching port 2A, a chromatography switching port 3A, a jumper switching port 1A, a jumper switching port 2A, a jumper switching port 3A, a sample port A and a cleaning port A, and a stator of the continuous flow chromatography component B is provided with a chromatography switching port 1B, a chromatography switching port 2B, a chromatography switching port 3B, A jumper switch port 1B, a jumper switch port 2B, a jumper switch port 3B, a sample port B and a cleaning port B.

Further, the chromatography switching port 1A and the chromatography switching port 1B are connected through a first chromatography channel, the chromatography switching port 2A and the chromatography switching port 2B are connected through a second chromatography channel, the chromatography switching port 3A and the chromatography switching port 3B are connected through a third chromatography channel, a first chromatography column, a second chromatography column and a third chromatography column are respectively and independently arranged in the first chromatography channel, the second chromatography channel and the third chromatography channel, the jumper switching port 1A and the jumper switching port 1B are connected through a first jumper channel, the jumper switching port 2A and the jumper switching port 2B are connected through a second jumper channel, the jumper switching port 3A and the jumper switching port 3B are connected through a third jumper channel, the sample port A is communicated with the sample channel A, the sample port B is communicated with the sample channel B, the cleaning port A is communicated with the cleaning solution channel A, the cleaning port B is communicated with a cleaning liquid channel B.

The invention also provides a continuous flow chromatography method, which comprises the following steps:

s1, the sample channel A is a sample inlet, the sample channel B is a sample outlet, the cleaning solution channel A is a cleaning solution outlet, the cleaning solution channel B is a cleaning solution inlet,

the switching pointer groove A is communicated with the chromatography switching port 1A and the sample port A, the bypass pointer groove A is communicated with the bypass groove A and the jumper switching port 3A, the first switching groove A is communicated with the jumper switching port 1A and the chromatography switching port 2A, the second switching groove A is communicated with the jumper switching port 2A and the chromatography switching port 3A, the bypass groove A is communicated with the bypass pointer groove A and the cleaning port A,

the switching pointer groove B is communicated with the chromatography switching port 3B and the sample port B, the bypass pointer groove B is communicated with the bypass groove B and the jumper switching port 3B, the first switching groove B is communicated with the jumper switching port 2B and the chromatography switching port 2B, the second switching groove B is communicated with the jumper switching port 1B and the chromatography switching port 1B, the bypass groove B is communicated with the bypass pointer groove B and the cleaning port B,

the sample sequentially passes through a sample channel A, a switching pointer groove A, a first chromatography channel, a first chromatography column, a second switching groove B, a first jumper channel, a first switching groove A, a second chromatography channel, a second chromatography column, a first switching groove B, a second jumper channel, a second switching groove A, a third chromatography channel, a third chromatography column, a switching pointer groove B and a sample channel B, and the cleaning solution sequentially passes through a cleaning solution channel B, a bypass groove B, a third jumper channel, a bypass groove A and a cleaning solution channel A;

s2, the rotor of the continuous flow chromatography component A rotates 120 degrees clockwise, a switching pointer groove A is communicated with a chromatography switching port 2A and a sample port A, a bypass pointer groove A is communicated with a bypass groove A and a jumper switching port 1A, a first switching groove A is communicated with the jumper switching port 2A and the chromatography switching port 3A, a second switching groove A is communicated with the jumper switching port 3A and the chromatography switching port 1A, the bypass groove A is communicated with the bypass pointer groove A and a cleaning port A,

the sample passes through the sample channel A, the switching pointer groove A, the second chromatography channel, the second chromatography column, the first switching groove B, the second jumper channel, the first switching groove A, the third chromatography channel, the third chromatography column, the switching pointer groove B and the sample channel B in sequence,

cleaning solution sequentially passes through a cleaning solution channel B, a bypass groove B, a bypass pointer groove B, a third jumper channel, a second switching groove A, a first chromatographic channel, a first chromatographic column, a second switching groove B, a first jumper channel, a bypass pointer groove A, a bypass groove A and a cleaning solution channel A, the first chromatographic column is cut out and connected with a cleaning pump, and the second chromatographic column and the third chromatographic column continue to sample;

s3, the rotor of the continuous flow chromatography component B rotates 120 degrees anticlockwise, the switching pointer groove B is communicated with the chromatography switching port 1B and the sample port B, the bypass pointer groove B is communicated with the bypass groove B and the jumper switching port 1B, the first switching groove B is communicated with the jumper switching port 3B and the chromatography switching port 3B, the second switching groove B is communicated with the jumper switching port 2B and the chromatography switching port 2B, the bypass groove B is communicated with the bypass pointer groove B and the cleaning port B,

the sample passes through a sample channel A, a switching pointer groove A, a second chromatography channel, a second chromatography column, a second switching groove B, a second jumper channel, a first switching groove A, a third chromatography channel, a third chromatography column, a first switching groove B, a third jumper channel, a second switching groove A, a first chromatography channel, a first chromatography column, a switching pointer groove B and a sample channel B in sequence,

the cleaning solution sequentially passes through a cleaning solution channel B, a bypass groove B, a bypass pointer groove B, a first jumper channel, a bypass pointer groove A, a bypass groove A and a cleaning solution channel A, the first chromatographic column is connected with the tail end of the return flow path, and the second chromatographic column, the third chromatographic column and the first chromatographic column are sequentially subjected to sample loading;

s4, the rotor of the continuous flow chromatography component A continuously rotates 120 degrees clockwise, the switching pointer groove A is communicated with the chromatography switching port 3A and the sample port A, the bypass pointer groove A is communicated with the bypass groove A and the jumper switching port 2A, the first switching groove A is communicated with the jumper switching port 3A and the chromatography switching port 1A, the second switching groove A is communicated with the jumper switching port 1A and the chromatography switching port 2A, the bypass groove A is communicated with the bypass pointer groove A and the cleaning port A,

the sample passes through the sample channel A, the switching pointer groove A, the third chromatography channel, the third chromatography column, the first switching groove B, the third jumper channel, the first switching groove A, the first chromatography channel, the first chromatography column, the switching pointer groove B and the sample channel B in sequence,

cleaning solution sequentially passes through a cleaning solution channel B, a bypass groove B, a bypass pointer groove B, a first jumper channel, a second switching groove A, a second chromatography channel, a second chromatography column, a second switching groove B, a second jumper channel, a bypass pointer groove A, a bypass groove A and a cleaning solution channel A, a cleaning pump is switched out of the second chromatography column, and a third chromatography column and the first chromatography column continue to sample;

s5, the rotor of the continuous flow chromatography component B continues to rotate 120 degrees anticlockwise, the switching pointer groove B is communicated with the chromatography switching port 2B and the sample port B, the bypass pointer groove B is communicated with the bypass groove B and the jumper switching port 2B, the first switching groove B is communicated with the jumper switching port 1B and the chromatography switching port 1B, the second switching groove B is communicated with the jumper switching port 3B and the chromatography switching port 3B, the bypass groove B is communicated with the bypass pointer groove B and the cleaning port B,

the sample passes through the sample channel A, the switching pointer groove A, the third chromatography channel, the third chromatography column, the second switching groove B, the third jumper channel, the first switching groove A, the first chromatography channel, the first chromatography column, the first switching groove B, the first jumper channel, the second switching groove A, the second chromatography channel, the second chromatography column, the switching pointer groove B and the sample channel B in sequence,

the cleaning solution sequentially passes through a cleaning solution channel B, a bypass groove B, a bypass pointer groove B, a second jumper channel, a bypass pointer groove A, a bypass groove A and a cleaning solution channel A, the second chromatographic column is connected with the tail end of the return flow path, and the third chromatographic column, the first chromatographic column and the second chromatographic column are sequentially subjected to sample loading;

s6, the rotor of the continuous flow chromatography component A rotates 120 degrees clockwise again, the switching pointer groove A is communicated with the chromatography switching port 1A and the sample port A, the bypass pointer groove A is communicated with the bypass groove A and the jumper switching port 3A, the first switching groove A is communicated with the jumper switching port 1A and the chromatography switching port 2A, the second switching groove A is communicated with the jumper switching port 2A and the chromatography switching port 3A, the bypass groove A is communicated with the bypass pointer groove A and the cleaning port A,

the sample passes through the sample channel A, the switching pointer groove A, the first chromatography channel, the first chromatography column, the first switching groove B, the first jumper channel, the first switching groove A, the second chromatography channel, the second chromatography column, the switching pointer groove B and the sample channel B in sequence,

cleaning solution sequentially passes through a cleaning solution channel B, a bypass groove B, a bypass pointer groove B, a second jumper channel, a second switching groove A, a third chromatography channel, a third chromatography column, a second switching groove B, a third jumper channel, a bypass pointer groove A, a bypass groove A and a cleaning solution channel A, a cleaning pump is switched out from the third chromatography column, and the first chromatography column and the second chromatography column are continuously loaded;

s7, the rotor of the continuous flow chromatography component B continues to rotate 120 degrees anticlockwise, the switching pointer groove B is communicated with the chromatography switching port 3B and the sample port B, the bypass pointer groove B is communicated with the bypass groove B and the jumper switching port 3B, the first switching groove B is communicated with the jumper switching port 2B and the chromatography switching port 2B, the second switching groove B is communicated with the jumper switching port 1B and the chromatography switching port 1B, the bypass groove B is communicated with the bypass pointer groove B and the cleaning port B,

the sample sequentially passes through a sample channel A, a switching pointer groove A, a first chromatography channel, a first chromatography column, a second switching groove B, a first jumper channel, a first switching groove A, a second chromatography channel, a second chromatography column, a first switching groove B, a second jumper channel, a second switching groove A, a third chromatography channel, a third chromatography column, a switching pointer groove B and a sample channel B, the cleaning solution sequentially passes through a cleaning solution channel B, a bypass groove B, a third jumper channel, a bypass groove A and a cleaning solution channel A, the third chromatography column is connected with the tail end of the flow path, the first chromatography column, the second chromatography column and the third chromatography column sequentially sample, the initial positions of the flow path and the rotor are the same as those in the step S1, and the steps are repeated to work circularly.

The invention also provides another continuous flow chromatography system which comprises two rotors, a stator plate, a jumper plate, two sample channels, two cleaning liquid channels, a plurality of chromatography columns, a sample loading pump and a cleaning pump, wherein the rotors rotate on the stator plate, the rotors are provided with a switching pointer groove, a bypass pointer groove, a switching groove and a bypass groove, the stator plate is provided with two groups of through opening combinations, the jumper plate is provided with a plurality of jumper channels, and the sequential cyclic switching of the jumper channels and the chromatography channels is realized through the clockwise or anticlockwise sequential rotation of the two rotors.

Furthermore, every jumper wire passageway of jumper board corresponds setting up under the stator board of two sets of opening combinations of intercommunication respectively, and every rotor corresponds a set of opening combination of intercommunication and sets up on the stator board, and every sample passageway, every washing liquid passageway correspond a set of opening combination of intercommunication respectively, and every chromatography passageway corresponds two sets of opening combinations of intercommunication respectively, sets up a chromatography post in every chromatography passageway.

Further, the opening combination includes that sample mouth, a plurality of chromatography switch over mouthful, a plurality of wire jumper switch over mouthful and wash the mouth, the chromatography switches over mouthful all to distribute along the rotation direction circumference with the wire jumper switch over mouthful, and the chromatography switches over mouthful and the wire jumper switches over a quantitative setting in turn such as mouthful, sample mouth and wash the mouth all with the chromatography switch over mouthful and the wire jumper switch over the non-coincidence of the distribution circumference of mouthful.

Further, every sample port communicates a sample passageway, and every washing liquid passageway of washing mouth intercommunication, every chromatography passageway both ends communicate the chromatography switching mouth of two opening combinations respectively, and a chromatography passageway is connected to every chromatography switching mouth of a set of opening combination, every jumper wire passageway both ends communicate the jumper wire switching mouth of two opening combinations respectively, and a jumper wire passageway is connected to every jumper wire switching mouth of a set of opening combination, be equipped with the appearance pump in the sample passageway, be equipped with the scavenging pump in the washing liquid passageway.

Furthermore, the sample channel and the cleaning liquid channel are arranged in the stator plate, a plurality of mutually non-communicated chromatography switching port channels are further arranged in the stator plate, each chromatography switching port channel is communicated with one chromatography switching port, and the chromatography channels are communicated with the chromatography switching ports through the chromatography switching port channels.

Furthermore, the switching pointer groove, the bypass pointer groove and the switching groove are arranged on the rotor in a non-communicated mode, the bypass pointer groove and the bypass groove are arranged on the rotor in a communicated mode, two ends of the switching pointer groove are respectively communicated with the sample port and the chromatography switching port, two ends of the bypass pointer groove are respectively communicated with the jumper switching port and the bypass groove, two ends of the switching groove are respectively communicated with the chromatography switching port and the jumper switching port, and the bypass groove is communicated with the bypass pointer groove and the cleaning port.

Further, be equipped with parallel first jumper board passageway of non-intercommunication, second jumper board passageway, third jumper board passageway on the jumper board, a plurality of chromatography columns include first chromatography column, second chromatography column, third chromatography column, two sets of opening combinations include opening combination A, opening combination B, and rotor A who corresponds the intercommunication with opening combination A has switching pointer groove A, bypass pointer groove A, first switching groove A, second switching groove A and bypass groove A, and rotor B who corresponds the intercommunication with opening combination B has switching pointer groove B, bypass pointer groove B, first switching groove B, second switching groove B and bypass groove B, opening combination A has chromatography switching mouth 1A, chromatography switching mouth 2A, chromatography switching mouth 3A, jumper switching mouth 1A, jumper switching mouth 2A, jumper switching mouth 3A, sample mouth A and washing mouth A, opening combination B has chromatography switching mouth 1B, chromatography switching mouth 1B, A chromatography switching port 2B, a chromatography switching port 3B, a jumper switching port 1B, a jumper switching port 2B, a jumper switching port 3B, a sample port B and a cleaning port B.

Further, the chromatography switching port 1A is connected with the chromatography switching port 1B through a first chromatography channel, the chromatography switching port 2A is connected with the chromatography switching port 2B through a second chromatography channel, the chromatography switching port 3A is connected with the chromatography switching port 3B through a third chromatography channel, a first chromatography column, a second chromatography column and a third chromatography column are respectively and independently arranged in the first chromatography channel, the second chromatography channel and the third chromatography channel, the jumper switching port 1A is connected with the jumper switching port 1B through a first jumper plate channel, the jumper switching port 2A is connected with the jumper switching port 2B through a second jumper plate channel, the jumper switching port 3A is connected with the jumper switching port 3B through a third jumper plate channel, the sample port A is communicated with the sample channel A, the sample port B is communicated with the sample channel B, and the cleaning port A is communicated with the cleaning solution channel A, the cleaning port B is communicated with a cleaning liquid channel B.

Compared with the prior art, the invention provides a continuous flow chromatography component, a system and a method, which have the following beneficial effects:

1. according to the continuous flow chromatography component, the chromatography system and the switching method, when adjustment is needed, switching between the leading-in connecting piece and the leading-out connecting piece and the interfaces can be achieved only by adjusting the movable adjusting piece, and the interfaces are switched and connected for use, so that different working states can be switched continuously.

2. The continuous flow chromatography component, the chromatography system and the switching method realize continuous sample loading of samples without stopping sample loading, the traditional step-by-step chromatography according to batches is not needed, the efficiency is effectively improved, only two adjusting parts are needed to rotate, a plurality of switching chromatographs are realized, and the chromatography efficiency is greatly improved.

3. The continuous flow chromatography assembly, the chromatography system and the switching method realize continuous flow of N columns by using only two switching assemblies, thereby reducing the number of the continuous flow chromatography assemblies, simplifying a flow path, facilitating control and reducing the fault occurrence rate.

Drawings

FIG. 1 is a first schematic structural diagram of a working state according to the present invention;

FIG. 2 is a second schematic structural diagram of the working state proposed by the present invention;

FIG. 3 is a third schematic structural view of the working state proposed by the present invention;

FIG. 4 is a fourth schematic structural view of the working state proposed by the present invention;

FIG. 5 is a fifth schematic structural view of the working state proposed by the present invention;

fig. 6 is a sixth schematic structural view of the working state proposed by the present invention;

FIG. 7 is a schematic structural diagram of a composite stator according to the present invention;

fig. 8 is a schematic structural diagram of an interposer according to the present invention;

fig. 9 is a schematic structural view of the assembly of the combined stator and the adapter plate according to the present invention;

fig. 10 is a schematic structural diagram of a stator a according to the present invention;

fig. 11 is a schematic structural diagram of a stator B according to the present invention;

fig. 12 is a schematic structural view of a rotor a according to the present invention;

fig. 13 is a schematic structural view of a rotor B according to the present invention;

FIG. 14 is a first schematic structural view of an adjustment member according to the present invention;

fig. 15 is a second schematic structural diagram of the adjusting member according to the present invention.

In the figure: 100. a continuous flow chromatography module A; 111. a chromatography switching port 1A; 112. a chromatography switching port 2A; 113. a chromatography switching port 3A; 114. a jumper switch port 1 a; 115. a jumper switch port 2 a; 116. a jumper switch port 3 a; 118. a sample port A; 119. cleaning the opening A; 120. a rotor A; 121. switching the pointer groove A; 122. a bypass pointer groove A; 123. a first switching slot A; 124. a second switching slot A; 125. a bypass groove A; 200. a continuous flow chromatography module B; 211. a chromatography switching port 1B; 212. a chromatography switching port 2B; 213. a chromatography switching port 3B; 214. a jumper switch port 1 b; 215. a jumper switching port 2 b; 216. a jumper switch port 3 b; 218. a sample port B; 219. cleaning the opening B; 220. a rotor B; 221. switching the pointer groove B; 222. a bypass pointer groove B; 223. a first switching slot B; 224. a second switching slot B; 225. a bypass groove B; 300. a jumper channel; 310. a first jumper channel; 320. a second jumper channel; 330. a third wire jumper channel; 400. a chromatography channel; 410. a first chromatography channel; 420. a second chromatography channel; 430. a third chromatography channel; 510. a sample channel A; 520. a sample channel B; 530. a sample loading pump; 610. a cleaning liquid channel A; 620. a cleaning liquid channel B; 630. cleaning the pump; 710. a first chromatography column; 720. a second chromatography column; 730. a third chromatography column; 800. a stator plate; 900. a jumper board; 910. a first jumper plate channel; 920. a second jumper plate channel; 930. a third patch panel channel.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Example (b):

referring to fig. 1-15, a continuous flow chromatography assembly comprises a rotor and a stator, wherein the rotor rotates on the stator, the rotor is provided with a switching pointer groove, a bypass pointer groove, a switching groove and a bypass groove, the stator is provided with a sample port, a chromatography switching port, a jumper switching port and a cleaning port, in the rotating process of the rotor, one end of the bypass pointer groove is communicated with the bypass groove, the other end of the bypass pointer groove is communicated with different jumper switching ports in a switching mode, one end of the switching pointer groove is communicated with the sample port, the other end of the switching pointer groove is communicated with different chromatography switching ports in a switching mode, one end of the switching groove is communicated with different chromatography switching ports in a switching mode, and the other end of the switching groove is communicated with different jumper switching ports in a switching mode.

The switching pointer groove, the bypass pointer groove and the switching groove are arranged on the rotor in a non-communicated mode, the bypass pointer groove and the bypass groove are arranged on the rotor in a communicated mode, the two ends of the switching pointer groove are respectively communicated with the sample port and the chromatography switching port, the two ends of the bypass pointer groove are respectively communicated with the jumper switching port and the bypass groove, the two ends of the switching groove are respectively communicated with the chromatography switching port and the jumper switching port, and the bypass groove is communicated with the bypass pointer groove and the cleaning port.

The switching pointer groove and the bypass pointer groove extend along the radial direction of the rotor, the switching groove is an arc-shaped groove extending along the circumferential direction of the rotor or a linear groove or a diagonal groove, the switching pointer groove and the bypass pointer groove are arranged adjacently, the switching groove is distributed and arranged along the circumferential direction of the rotor, the chromatography switching port and the jumper switching port are circumferentially distributed along the rotating direction, the chromatography switching port and the jumper switching port are alternately arranged in an equal number mode, and the sample port and the cleaning port are not overlapped with the distribution circumference of the chromatography switching port and the jumper switching port.

The distribution circumference of the chromatography switching port and the distribution circumference of the jumper switching port are equal in diameter, the sample port is positioned in the circle center position in the distribution circumference, the cleaning port is positioned outside the distribution circumference, the bypass groove is closed and surrounds the periphery of the rotor, or the cleaning port is positioned in the distribution circumference, and the bypass groove is not closed and surrounds the center of the rotor.

Referring to fig. 1-6, the continuous flow chromatography system further includes M jumper channels 300, N chromatography channels 400, a sample channel, a cleaning solution channel, a chromatography column, a sample loading pump 530, and a cleaning pump 630, where M is greater than or equal to 1, N is greater than or equal to 1, and the sequential cyclic switching of the M jumper channels 300 and the N chromatography channels 400 is realized by the clockwise or counterclockwise sequential rotation of rotors of the two continuous flow chromatography components.

The two ends of each chromatography channel are respectively connected with the chromatography switching ports of the two switching assemblies, each chromatography switching port of a single switching assembly is correspondingly connected with one chromatography channel, a chromatography column is arranged in each chromatography channel, the two ends of each jumper channel 300 are respectively and sequentially connected with the jumper switching ports of the two switching assemblies, each jumper switching port of the single switching assembly is correspondingly connected with one jumper channel 300, the sample channels are communicated with the sample ports, the cleaning liquid channels are communicated with the cleaning ports, the sample channels are internally provided with sample loading pumps 530, and the cleaning liquid channels are internally provided with cleaning pumps 630.

The number of the chromatographic columns is N, the number of the N is 2 or 3 or 4 or 6, the number of the chromatographic switching ports and the number of the jumper switching ports of the switching assembly are the same as the number of the chromatographic columns, the number M of the jumper channels is 300 and the number N of the chromatographic channels is 400, the angles of the chromatographic switching ports and the jumper switching ports which are uniformly distributed along the circumference are 360 degrees/N, and the number of the switching grooves of the switching assembly is N-1.

The continuous flow chromatography system further comprises a first chromatography column 710, a second chromatography column 720, a third chromatography column 730, a continuous flow chromatography component A100 and a continuous flow chromatography component B200, wherein a rotor A120 of the continuous flow chromatography component A100 is provided with a switching pointer groove A121, a bypass pointer groove A122, a first switching groove A123, a second switching groove A124 and a bypass groove A125, a rotor B220 of the continuous flow chromatography component B200 is provided with a switching pointer groove B221, a bypass pointer groove B222, a first switching groove B223, a second switching groove B224 and a bypass groove B225, a stator of the continuous flow chromatography component A100 is provided with a chromatography switching port 1A111, a chromatography switching port 2A112, a chromatography switching port 3A113, a jumper switching port 1a114, a jumper switching port 2a115, a jumper switching port 3a116, a sample port A118 and a cleaning port A119, and a stator of the chromatography component B200 is provided with a chromatography switching port 1B211, a chromatography switching port 2B212, a chromatography switching port 3B213, a jumper switching port 214, Jumper switch port 2B215, jumper switch port 3B216, sample port B218, and purge port B219.

The chromatography switching port 1A111 and the chromatography switching port 1B211 are connected by a first chromatography channel 410, the chromatography switching port 2A112 and the chromatography switching port 2B212 are connected by a second chromatography channel 420, the chromatography switching port 3A113 and the chromatography switching port 3B213 are connected by a third chromatography channel 430, the first chromatography column 710 is separately arranged in the first chromatography channel 410, the second chromatography channel 420 and the third chromatography channel 430, the second chromatographic column 720 and the third chromatographic column 730, the jumper switch port 1a114 and the jumper switch port 1B214 are connected through a first jumper channel 310, the jumper switch port 2a115 and the jumper switch port 2B215 are connected through a second jumper channel 320, the jumper switch port 3a116 and the jumper switch port 3B216 are connected through a third jumper channel 330, the sample port A118 is communicated with a sample channel A510, the sample port B218 is communicated with a sample channel B520, the cleaning port A119 is communicated with a cleaning solution channel A610, and the cleaning port B219 is communicated with a cleaning solution channel B620.

A method of continuous flow chromatography comprising the steps of:

s1, a sample channel A510 is a sample inlet, a sample channel B520 is a sample outlet, a cleaning solution channel A610 is a cleaning solution outlet, a cleaning solution channel B620 is a cleaning solution inlet,

the switching finger groove A121 communicates the chromatographic switching port 1A111 with the sample port A118, the bypass finger groove A122 communicates the bypass groove A125 with the jumper switching port 3a116, the first switching groove A123 communicates the jumper switching port 1a114 with the chromatographic switching port 2A112, the second switching groove A124 communicates the jumper switching port 2a115 with the chromatographic switching port 3A113, the bypass groove A125 communicates the bypass finger groove A122 with the wash port A119,

the switching finger groove B221 communicates the chromatography switching port 3B213 with the sample port B218, the bypass finger groove B222 communicates the bypass groove B225 with the jumper switching port 3B216, the first switching groove B223 communicates the jumper switching port 2B215 with the chromatography switching port 2B212, the second switching groove B224 communicates the jumper switching port 1B214 with the chromatography switching port 1B211, the bypass groove B225 communicates the bypass finger groove B222 with the wash port B219,

the sample sequentially passes through a sample channel A510, a switching pointer groove A121, a first chromatography channel 410, a first chromatography column 710, a second switching groove B224, a first jumper channel 310, a first switching groove A123, a second chromatography channel 420, a second chromatography column 720, a first switching groove B223, a second jumper channel 320, a second switching groove A124, a third chromatography channel 430, a third chromatography column 730, a switching pointer groove B221 and a sample channel B520, and the cleaning solution sequentially passes through a cleaning solution channel B620, a bypass groove B225, a third jumper channel 330, a bypass groove A125 and a cleaning solution channel A610;

s2. the rotor of the continuous flow chromatography component A100 rotates 120 degrees clockwise, the switching pointer groove A121 is communicated with the chromatography switching port 2A112 and the sample port A118, the bypass pointer groove A122 is communicated with the bypass groove A125 and the jumper switching port 1a114, the first switching groove A123 is communicated with the jumper switching port 2a115 and the chromatography switching port 3A113, the second switching groove A124 is communicated with the jumper switching port 3a116 and the chromatography switching port 1A111, the bypass groove A125 is communicated with the bypass pointer groove A122 and the cleaning port A119,

the sample passes through the sample channel A510, the switching pointer groove A121, the second chromatography channel 420, the second chromatography column 720, the first switching groove B223, the second jumper channel 320, the first switching groove A123, the third chromatography channel 430, the third chromatography column 730, the switching pointer groove B221 and the sample channel B520 in sequence,

the cleaning solution sequentially passes through a cleaning solution channel B620, a bypass groove B225, a bypass pointer groove B222, a third jumper channel 330, a second switching groove A124, a first chromatographic channel 410, a first chromatographic column 710, a second switching groove B224, a first jumper channel 310, a bypass pointer groove A122, a bypass groove A125 and a cleaning solution channel A610, the first chromatographic column 710 is cut out and connected with a cleaning pump 630, and the second chromatographic column 720 and the third chromatographic column 730 continue to sample;

s3. the rotor of the continuous flow chromatography component B200 rotates 120 degrees counterclockwise, the switching indicator groove B221 is communicated with the chromatography switching port 1B211 and the sample port B218, the bypass indicator groove B222 is communicated with the bypass groove B225 and the jumper switching port 1B214, the first switching groove B223 is communicated with the jumper switching port 3B216 and the chromatography switching port 3B213, the second switching groove B224 is communicated with the jumper switching port 2B215 and the chromatography switching port 2B212, the bypass groove B225 is communicated with the bypass indicator groove B222 and the cleaning port B219,

the sample passes through the sample channel A510, the switching pointer groove A121, the second chromatography channel 420, the second chromatography column 720, the second switching groove B224, the second jumper channel 320, the first switching groove A123, the third chromatography channel 430, the third chromatography column 730, the first switching groove B223, the third jumper channel 330, the second switching groove A124, the first chromatography channel 410, the first chromatography column 710, the switching pointer groove B221 and the sample channel B520 in sequence,

the cleaning solution passes through the cleaning solution channel B620, the bypass groove B225, the bypass indicator groove B222, the first jumper channel 310, the bypass indicator groove A122, the bypass groove A125 and the cleaning solution channel A610 in sequence, the first chromatographic column 710 is connected back to the tail end of the flow path, and the second chromatographic column 720, the third chromatographic column 730 and the first chromatographic column 710 are sequentially loaded with the sample;

s4, the rotor of the continuous flow chromatography component A100 rotates 120 degrees clockwise, the switching pointer groove A121 is communicated with the chromatography switching port 3A113 and the sample port A118, the bypass pointer groove A122 is communicated with the bypass groove A125 and the jumper switching port 2a115, the first switching groove A123 is communicated with the jumper switching port 3a116 and the chromatography switching port 1A111, the second switching groove A124 is communicated with the jumper switching port 1a114 and the chromatography switching port 2A112, the bypass groove A125 is communicated with the bypass pointer groove A122 and the cleaning port A119,

the sample passes through the sample channel A510, the switching pointer groove A121, the third chromatography channel 430, the third chromatography column 730, the first switching groove B223, the third jumper channel 330, the first switching groove A123, the first chromatography channel 410, the first chromatography column 710, the switching pointer groove B221 and the sample channel B520 in sequence,

the cleaning solution sequentially passes through a cleaning solution channel B620, a bypass groove B225, a bypass indicator groove B222, a first jumper channel 310, a second switching groove A124, a second chromatography channel 420, a second chromatography column 720, a second switching groove B224, a second jumper channel 320, a bypass indicator groove A122, a bypass groove A125 and a cleaning solution channel A610, the second chromatography column 720 is cut out and connected with a cleaning pump 630, and the third chromatography column 730 and the first chromatography column 710 continue to sample;

s5, the rotor of the continuous flow chromatography component B200 rotates 120 degrees counterclockwise continuously, a switching pointer groove B221 is communicated with a chromatography switching port 2B212 and a sample port B218, a bypass pointer groove B222 is communicated with a bypass groove B225 and a jumper switching port 2B215, a first switching groove B223 is communicated with a jumper switching port 1B214 and a chromatography switching port 1B211, a second switching groove B224 is communicated with a jumper switching port 3B216 and a chromatography switching port 3B213, a bypass groove B225 is communicated with a bypass pointer groove B222 and a cleaning port B219,

the sample passes through the sample channel A510, the switch pointer groove A121, the third chromatography channel 430, the third chromatography column 730, the second switch groove B224, the third jumper channel 330, the first switch groove A123, the first chromatography channel 410, the first chromatography column 710, the first switch groove B223, the first jumper channel 310, the second switch groove A124, the second chromatography channel 420, the second chromatography column 720, the switch pointer groove B221 and the sample channel B520 in sequence,

the cleaning solution passes through the cleaning solution channel B620, the bypass groove B225, the bypass pointer groove B222, the second jumper channel 320, the bypass pointer groove A122, the bypass groove A125 and the cleaning solution channel A610 in sequence, the second chromatographic column 720 is connected back to the tail end of the flow path, and the third chromatographic column 730, the first chromatographic column 710 and the second chromatographic column 720 are sequentially loaded with the sample;

s6, the rotor of the continuous flow chromatography component A100 rotates 120 degrees clockwise again, the switching pointer groove A121 is communicated with the chromatography switching port 1A111 and the sample port A118, the bypass pointer groove A122 is communicated with the bypass groove A125 and the jumper switching port 3a116, the first switching groove A123 is communicated with the jumper switching port 1a114 and the chromatography switching port 2A112, the second switching groove A124 is communicated with the jumper switching port 2a115 and the chromatography switching port 3A113, the bypass groove A125 is communicated with the bypass pointer groove A122 and the cleaning port A119,

the sample passes through the sample channel A510, the switching indicator groove A121, the first chromatography channel 410, the first chromatography column 710, the first switching groove B223, the first jumper channel 310, the first switching groove A123, the second chromatography channel 420, the second chromatography column 720, the switching indicator groove B221, and the sample channel B520 in sequence,

the cleaning solution sequentially passes through a cleaning solution channel B620, a bypass groove B225, a bypass pointer groove B222, a second jumper channel 320, a second switching groove A124, a third chromatography channel 430, a third chromatography column 730, a second switching groove B224, a third jumper channel 330, a bypass pointer groove A122, a bypass groove A125 and a cleaning solution channel A610, the third chromatography column 730 is cut out and connected with a cleaning pump 630, and the first chromatography column 710 and the second chromatography column 720 continue to sample;

s7, the rotor of the continuous flow chromatography component B200 rotates 120 degrees counterclockwise continuously, a switching pointer groove B221 is communicated with a chromatography switching port 3B213 and a sample port B218, a bypass pointer groove B222 is communicated with a bypass groove B225 and a jumper switching port 3B216, a first switching groove B223 is communicated with a jumper switching port 2B215 and a chromatography switching port 2B212, a second switching groove B224 is communicated with a jumper switching port 1B214 and a chromatography switching port 1B211, a bypass groove B225 is communicated with a bypass pointer groove B222 and a cleaning port B219,

the sample passes through the sample channel A510, the switching indicator groove A121, the first chromatography channel 410, the first chromatography column 710, the second switching groove B224, the first jumper channel 310, the first switching groove A123, the second chromatography channel 420, the second chromatography column 720, the first switching groove B223, the second jumper channel 320, the second switching groove A124, the third chromatography channel 430, the third chromatography column 730, the switching indicator groove B221, and the sample channel B520 in sequence, the washing solution passes through the washing solution channel B620, the bypass groove B225, the third jumper channel 330, the bypass groove A125, and the washing solution channel A610 in sequence, the third chromatography column 730 is connected to the end of the flow path, the first chromatography column 710, the second chromatography column 720, and the third chromatography column 730 are sequentially loaded, the flow path and the initial position of the rotor are the same as those in the step S1, and the above steps are repeated to work in a cycle.

In order to prevent the mutual streaming of the fluids in different grooves and improve the reliability and safety of switching communication, the ends of the ports and the grooves are in sealed communication, and the sealing modes include but are not limited to sealing, bonding and thermoplastic sealing.

In order to improve the stability of the channel connection, the channel is fixed on the stator and communicated with the port, and the fixing way of the channel and the stator includes but is not limited to: the passage penetrating the stator body and the independent passage fixedly connected outside the stator body are not described in detail.

Referring to fig. 7-15, a continuous flow chromatography system, including two rotors, stator board 800, jumper board 900, two sample channels, two cleaning fluid channels, a plurality of chromatography columns, sample loading pump 530, cleaning pump 630, the rotors rotate on stator board 800, characterized in that, the rotors have switching finger groove, bypass finger groove, switching groove and bypass groove, stator board 800 has two sets of through-opening combinations, each rotor corresponds to communicate a set of through-opening combination to set up on stator board 800, jumper board 900 is equipped with a plurality of jumper channels, through clockwise or anticlockwise rotation in proper order of two rotors, realize the circulation switching in proper order of a plurality of jumper channels, a plurality of chromatography channels.

Every jumper wire passageway of jumper board 900 corresponds setting up under stator board 800 of two sets of openings combinations of intercommunication respectively, and stator board 800 superposes from last to down in proper order with jumper board 900, and the lower surface of stator board 800 and the last surface seal laminating of jumper board 900, and the sealing method includes and is not limited to sealing member seal, bonding seal, heat and moulds sealedly.

And each sample channel and each cleaning liquid channel are respectively and correspondingly communicated with a group of through port combinations, each chromatography channel is respectively and correspondingly communicated with two groups of through port combinations, and each chromatography channel is internally provided with a chromatography column.

The opening combination includes that sample mouth, a plurality of chromatography switch mouthful, a plurality of wire jumper switch mouthful and wash the mouth, and the chromatography switches mouthful all distributes along the rotation direction circumference with the wire jumper switch mouthful, and the chromatography switches mouthful and the wire jumper switches the equal quantity of mouthful and sets up in turn, and sample mouth and wash mouthful all with the chromatography switch mouthful and the wire jumper switch mouthful distribution circumference nonconformity, and the chromatography switches mouthful locates on the stator board, and the wire jumper switches mouthful locates on the jumper board.

Every sample port communicates a sample passageway, every washing mouth communicates a washing liquid passageway, every chromatography passageway both ends communicate the chromatography switching mouth of two opening combinations respectively, a chromatography passageway is connected to every chromatography switching mouth of a set of opening combination, the wire jumper switching mouth of two opening combinations is communicated respectively at every wire jumper passageway both ends, a wire jumper passageway is connected to every wire jumper switching mouth of a set of opening combination, be equipped with sample loading pump 530 in the sample passageway, be equipped with cleaning pump 630 in the washing liquid passageway.

In sample passageway, washing liquid passageway located stator board 800, still be provided with the chromatography switching mouth passageway of a plurality of mutual non-intercommunications in the stator board 800, every chromatography switching mouth passageway intercommunication chromatography switching mouth, the chromatography passageway switches a mouth through chromatography switching mouth passageway intercommunication chromatography, is equipped with the stator board opening that the intercommunication wire jumper switched the mouth on the stator board 800, stator board opening and wire jumper switch a mouthful one-to-one setting.

As an embodiment, a continuous flow chromatography system, including two rotors, the stator board, two sample passageways, two washing liquid passageways, a plurality of chromatography columns, the pump of getting ready, the scavenging pump, the rotor rotates on the stator board, the rotor has the switching pointer groove, bypass pointer groove, switching groove and bypass groove, the stator board has two sets of opening combinations, every rotor corresponds the combination of a set of opening of intercommunication and sets up on the stator board, the stator board is equipped with the jumper wire passageway of a plurality of indirect intercommunications, clockwise or anticlockwise rotation in proper order through two rotors, realize a plurality of jumper wire passageways, the circulation in proper order of a plurality of chromatography passageways switches, every jumper wire passageway corresponds the combination of two sets of openings of intercommunication respectively, chromatography switching mouth and jumper wire switching mouth all locate on the stator board.

The jumper plate 900 is provided with a first jumper plate channel 910, a second jumper plate channel 920 and a third jumper plate channel 930 which are not communicated in parallel, the plurality of chromatographic columns comprise a first chromatographic column 710, a second chromatographic column 720 and a third chromatographic column 730, the two sets of port combinations comprise a port combination A and a port combination B, a rotor A120 correspondingly communicated with the port combination A is provided with a switching pointer groove A121, a bypass pointer groove A122, a first switching groove A123, a second switching groove A124 and a bypass groove A125, a rotor B220 correspondingly communicated with the port combination B is provided with a switching pointer groove B221, a bypass pointer groove B222, a first switching groove B223, a second switching groove B224 and a bypass groove B225, the port combination A is provided with a chromatographic switching port 1A111, a chromatographic switching port 2A112, a chromatographic switching port 3A113, a jumper switching port 1a114, a jumper switching port 2a115, a jumper switching port 3a116, a sample port A118 and a cleaning port A119, and the port combination B is provided with a chromatographic switching port 1A111, and the port B211 is provided with a chromatographic switching port 1B switching port 1, Chromatography switching port 2B212, chromatography switching port 3B213, jumper switching port 1B214, jumper switching port 2B215, jumper switching port 3B216, sample port B218, and wash port B219.

The chromatography switching port 1A111 and the chromatography switching port 1B211 are connected by a first chromatography channel 410, the chromatography switching port 2A112 and the chromatography switching port 2B212 are connected by a second chromatography channel 420, the chromatography switching port 3A113 and the chromatography switching port 3B213 are connected by a third chromatography channel 430, the first chromatography column 710 is separately arranged in the first chromatography channel 410, the second chromatography channel 420 and the third chromatography channel 430, the second chromatographic column 720 and the third chromatographic column 730, the jumper switching port 1a114 is connected with the jumper switching port 1B214 through a first jumper plate channel 910, the jumper switching port 2a115 is connected with the jumper switching port 2B215 through a second jumper plate channel 920, the jumper switching port 3a116 is connected with the jumper switching port 3B216 through a third jumper plate channel 930, the sample port A118 is communicated with the sample channel A510, the sample port B218 is communicated with the sample channel B520, the cleaning port A119 is communicated with the cleaning solution channel A610, and the cleaning port B219 is communicated with the cleaning solution channel B620.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A continuous flow chromatography component comprises a rotor and a stator, wherein the rotor rotates on the stator, and the continuous flow chromatography component is characterized in that the rotor is provided with a switching pointer groove, a bypass pointer groove, a switching groove and a bypass groove, the stator is provided with a sample port, a chromatography switching port, a jumper switching port and a cleaning port, in the rotating process of the rotor, one end of the bypass pointer groove is communicated with the bypass groove, the other end of the bypass pointer groove is communicated with different jumper switching ports in a switching mode, one end of the switching pointer groove is communicated with the sample port, the other end of the switching pointer groove is communicated with different chromatography switching ports in a switching mode, one end of the switching groove is communicated with different chromatography switching ports in a switching mode, and the other end of the switching groove is communicated with different jumper switching ports in a switching mode.

2. The continuous flow chromatography assembly according to claim 1, wherein the switching finger slot, the bypass finger slot and the switching slot are disposed on the rotor in a non-communicating manner, the bypass finger slot and the bypass slot are disposed on the rotor in a communicating manner, two ends of the switching finger slot are respectively communicated with the sample port and the chromatography switching port, two ends of the bypass finger slot are respectively communicated with the jumper switching port and the bypass slot, two ends of the switching slot are respectively communicated with the chromatography switching port and the jumper switching port, and the bypass slot is communicated with the bypass finger slot and the cleaning port.

3. The continuous flow chromatography assembly according to claim 2, wherein the switching finger slot and the bypass finger slot extend along a radial direction of the rotor, the switching slot is an arc slot extending along a circumferential direction of the rotor, a straight slot or a diagonal slot, the switching finger slot and the bypass finger slot are disposed adjacent to each other, the switching slots are disposed along the circumferential direction of the rotor, the chromatography switching ports and the jumper switching ports are circumferentially disposed along a rotation direction, the chromatography switching ports and the jumper switching ports are alternately disposed in equal number, and the sample ports and the cleaning ports are non-coincident with a distribution circumference of the chromatography switching ports and the jumper switching ports.

4. The continuous flow chromatography assembly of claim 3, wherein the chromatography switching port distribution circumference is of the same diameter as the jumper switching port distribution circumference, the sample port is located at a circle center position within the distribution circumference, the purge port is located outside the distribution circumference, and the bypass channel is closed around the periphery of the rotor, or the purge port is located within the distribution circumference, and the bypass channel is non-closed around the center of the rotor.

5. A continuous flow chromatography system having the continuous flow chromatography module according to any of claims 1-4, further comprising M jumper channels (300), N chromatography channels (400), a sample channel, a wash liquid channel, a plurality of chromatography columns, a loading pump (530), and a wash pump (630), wherein M is greater than or equal to 1, N is greater than or equal to 1, and the number of continuous flow chromatography modules is two, and sequential cyclic switching of the M jumper channels (300) and the N chromatography channels (400) is realized by sequential clockwise or counterclockwise rotation of two rotors of the continuous flow chromatography module.

6. The continuous flow chromatography system according to claim 5, wherein the two ends of each chromatography channel of the chromatography channel (400) are respectively connected with the chromatography switching ports of two switching assemblies, each chromatography switching port of a single switching assembly is correspondingly connected with one chromatography channel, a chromatography column is arranged in each chromatography channel, the two ends of each jumper channel of the jumper channel (300) are respectively connected with the jumper switching ports of two switching assemblies in turn, each jumper switching port of a single switching assembly is correspondingly connected with one jumper channel (300), the sample channel is communicated with the sample port, the cleaning solution channel is communicated with the cleaning port, the sample channel is provided with a sample feeding pump (530), and the cleaning solution channel is provided with a cleaning pump (630).

7. The continuous flow chromatography system according to claim 5, wherein the number of chromatography columns is N, N is 2 or 3 or 4 or 6, the number of chromatography switching ports and jumper switching ports of the switching assembly is the same as the number of chromatography columns, the number M of jumper channels (300) is the same as the number N of chromatography channels (400), the chromatography switching ports and jumper switching ports are circumferentially arranged at an angle of 360 °/N, and the number of switching slots of the switching assembly is N-1.

8. The continuous flow chromatography system of claim 5, further comprising a first chromatography column (710), a second chromatography column (720), a third chromatography column (730), a continuous flow chromatography module A (100), a continuous flow chromatography module B (200), wherein the rotor A (120) of the continuous flow chromatography module A (100) has a switching finger slot A (121), a bypass finger slot A (122), a first switching slot A (123), a second switching slot A (124) and a bypass continuous flow slot A (125), the rotor B (220) of the continuous flow chromatography module B (200) has a switching finger slot B (221), a bypass finger slot B (222), a first switching slot B (223), a second switching slot B (224) and a bypass slot B (225), and the stator of the continuous flow chromatography module A (100) has a chromatography switching port 1A (111), a chromatography switching port 2A (112), The stator of the continuous flow chromatography component B (200) is provided with a chromatography switching port 1B (211), a chromatography switching port 2B (212), a chromatography switching port 3B (213), a jumper switching port 1B (214), a jumper switching port 2B (215), a jumper switching port 3B (216), a sample port B (218) and a cleaning port B (219).

9. The continuous flow chromatography system of claim 8, wherein the chromatography switching port 1A (111) and the chromatography switching port 1B (211) are connected by a first chromatography channel (410), the chromatography switching port 2A (112) and the chromatography switching port 2B (212) are connected by a second chromatography channel (420), the chromatography switching port 3A (113) and the chromatography switching port 3B (213) are connected by a third chromatography channel (430), and the first chromatography channel (410), the second chromatography channel (420) and the third chromatography channel (430) are respectively provided with the first chromatography column (710), the second chromatography column (720) and the third chromatography column (730) separately.

10. The continuous flow chromatography system of claim 9, wherein the jumper switch port 1a (114) and jumper switch port 1B (214) are connected by a first jumper channel (310), the jumper switch port 2a (115) and jumper switch port 2B (215) are connected by a second jumper channel (320), the jumper switch port 3a (116) and jumper switch port 3B (216) are connected by a third jumper channel (330), the sample port A (118) is connected to sample channel A (510), the sample port B (218) is connected to sample channel B (520), the purge port A (119) is connected to purge channel A (610), and the purge port B (219) is connected to purge channel B (620).

11. A continuous flow chromatography method for performing a column switching operation using the continuous flow chromatography system according to any of claims 8 to 10, comprising the steps of:

s1, the sample channel A (510) is a sample inlet, the sample channel B (520) is a sample outlet, the cleaning solution channel A (610) is a cleaning solution outlet, the cleaning solution channel B (620) is a cleaning solution inlet, a sample sequentially passes through the sample channel A (510), the switching pointer groove A (121), the first chromatography channel (410), the first chromatography column (710), the second switching groove B (224), the first jumper channel (310), the first switching groove A (123), the second chromatography channel (420), the second chromatography column (720), the first switching groove B (223), the second jumper channel (320), the second switching groove A (124), the third chromatography channel (430), the third chromatography column (730), the switching pointer groove B (221) and the sample channel B (520), and a cleaning solution sequentially passes through the cleaning solution channel B (620), the bypass groove B (225), the third jumper channel (330), A bypass groove A (125), a cleaning liquid passage A (610);

s2, the rotor of the continuous flow chromatography component A (100) rotates 120 degrees clockwise, a sample sequentially passes through a sample channel A (510), a switching pointer groove A (121), a second chromatography channel (420), a second chromatography column (720), a first switching groove B (223), a second jumper channel (320), a first switching groove A (123), a third chromatography channel (430), a third chromatography column (730), a switching pointer groove B (221) and a sample channel B (520), and a cleaning solution sequentially passes through a cleaning solution channel B (620), a bypass groove B (225), a bypass pointer groove B (222), a third jumper channel (330), a second switching groove A (124), a first chromatography channel (410), a first chromatography column (710), a second switching groove B (224), a first jumper channel (310), a bypass pointer groove A (122), a bypass groove A (125) and a cleaning solution channel A (610), the first chromatographic column (710) is cut out and connected with a cleaning pump (630), and the second chromatographic column (720) and the third chromatographic column (730) continue to sample;

s3, rotating a rotor of the continuous flow chromatography component B (200) by 120 degrees anticlockwise, enabling a sample to sequentially pass through a sample channel A (510), a switching pointer groove A (121), a second chromatography channel (420), a second chromatography column (720), a second switching groove B (224), a second jumper channel (320), a first switching groove A (123), a third chromatography channel (430), a third chromatography column (730), a first switching groove B (223), a third jumper channel (330), a second switching groove A (124), a first chromatography channel (410), a first chromatography column (710), a switching pointer groove B (221) and a sample channel B (520), enabling a cleaning solution to sequentially pass through a cleaning solution channel B (620), a bypass groove B (225), a bypass pointer groove B (222), a first jumper channel (310), a bypass pointer groove A (122), a bypass groove A (125) and a cleaning solution channel A (610), the first chromatographic column (710) is connected back to the end of the flow path, and the second chromatographic column (720), the third chromatographic column (730) and the first chromatographic column (710) are sequentially loaded;

s4, the rotor of the continuous flow chromatography component A (100) continuously rotates 120 degrees clockwise, a sample sequentially passes through a sample channel A (510), a switching pointer groove A (121), a third chromatography channel (430), a third chromatography column (730), a first switching groove B (223), a third jumper channel (330), a first switching groove A (123), a first chromatography channel (410), a first chromatography column (710), a switching pointer groove B (221) and a sample channel B (520), and a cleaning solution sequentially passes through a cleaning solution channel B (620), a bypass groove B (225), a bypass pointer groove B (222), a first jumper channel (310), a second switching groove A (124), a second chromatography channel (420), a second chromatography column (720), a second switching groove B (224), a second jumper channel (320), a bypass pointer groove A (122), a bypass groove A (125) and a cleaning solution channel A (610), the second chromatographic column (720) is cut out and connected with a cleaning pump (630), and the third chromatographic column (730) and the first chromatographic column (710) continue to sample;

s5, the rotor of the continuous flow chromatography component B (200) continuously rotates 120 degrees counterclockwise, a sample sequentially passes through a sample channel A (510), a switching pointer groove A (121), a third chromatography channel (430), a third chromatography column (730), a second switching groove B (224), a third jumper channel (330), a first switching groove A (123), a first chromatography channel (410), a first chromatography column (710), a first switching groove B (223), a first jumper channel (310), a second switching groove A (124), a second chromatography channel (420), a second chromatography column (720), a switching pointer groove B (221) and a sample channel B (520), and a cleaning solution sequentially passes through a cleaning solution channel B (620), a bypass groove B (225), a bypass pointer groove B (222), a second jumper channel (320), a bypass pointer groove A (122), a bypass groove A (125) and a cleaning solution channel A (610), the second chromatography column (720) is connected back to the end of the flow path, and the third chromatography column (730), the first chromatography column (710) and the second chromatography column (720) are sequentially loaded;

s6, the rotor of the continuous flow chromatography component A (100) rotates 120 degrees clockwise again, a sample sequentially passes through a sample channel A (510), a switching pointer groove A (121), a first chromatography channel (410), a first chromatography column (710), a first switching groove B (223), a first jumper channel (310), a first switching groove A (123), a second chromatography channel (420), a second chromatography column (720), a switching pointer groove B (221) and a sample channel B (520), and a cleaning solution sequentially passes through a cleaning solution channel B (620), a bypass groove B (225), a bypass pointer groove B (222), a second jumper channel (320), a second switching groove A (124), a third chromatography channel (430), a third chromatography column (730), a second switching groove B (224), a third jumper channel (330), a bypass pointer groove A (122), a bypass groove A (125) and a cleaning solution channel A (610), the third chromatographic column (730) is cut out and connected with a cleaning pump (630), and the first chromatographic column (710) and the second chromatographic column (720) continue to sample;

s7, the rotor of the continuous flow chromatography component B (200) continuously rotates 120 degrees in the anticlockwise direction, a sample sequentially passes through a sample channel A (510), a switching pointer groove A (121), a first chromatography channel (410), a first chromatography column (710), a second switching groove B (224), a first jumper channel (310), a first switching groove A (123), a second chromatography channel (420), a second chromatography column (720), a first switching groove B (223), a second jumper channel (320), a second switching groove A (124), a third chromatography channel (430), a third chromatography column (730), a switching pointer groove B (221) and a sample channel B (520), a cleaning solution sequentially passes through a cleaning solution channel B (620), a bypass groove B (225), a third jumper channel (330), a bypass groove A (125) and a cleaning solution channel A (610), the third chromatography column (730) is connected back to the tail end of a flow path, the first chromatography column (710), The second column (720) and the third column (730) are sequentially loaded, the flow path and the rotor initial position are the same as those in step S1, and the above steps are repeated to perform the cycle operation.

12. The continuous flow chromatography method according to claim 11, wherein in step S1, the switching finger groove A (121) communicates the chromatography switching port 1A (111) with the sample port A (118), the bypass finger groove A (122) communicates the bypass groove A (125) with the jumper switching port 3A (116), the first switching groove A (123) communicates the jumper switching port 1A (114) with the chromatography switching port 2A (112), the second switching groove A (124) communicates the jumper switching port 2A (115) with the chromatography switching port 3A (113), the bypass groove A (125) communicates the bypass finger groove A (122) with the cleaning port A (119), the switching finger groove B (221) communicates the chromatography switching port 3B (213) with the sample port B (218), the finger groove B (222) communicates the bypass groove B (225) with the jumper switching port 3B (216), and the first switching groove B (223) communicates the jumper switching port 2B (215) with the chromatography switching port 2B (212), the second switching groove B (224) is communicated with the jumper switching port 1B (214) and the chromatography switching port 1B (211), and the bypass groove B (225) is communicated with the bypass pointer groove B (222) and the cleaning port B (219);

in step S2, the rotor of the continuous flow chromatography module a (100) is switched by rotation, the switching pointer groove a (121) communicates the chromatography switching port 2A (112) and the sample port a (118), the bypass pointer groove a (122) communicates the bypass groove a (125) and the jumper switching port 1A (114), the first switching groove a (123) communicates the jumper switching port 2A (115) and the chromatography switching port 3A (113), the second switching groove a (124) communicates the jumper switching port 3A (116) and the chromatography switching port 1A (111), and the bypass groove a (125) communicates the bypass pointer groove a (122) and the cleaning port a (119);

in step S3, the rotor of the continuous flow chromatography module B (200) is rotationally switched, the switching pointer groove B (221) communicates the chromatography switching port 1B (211) and the sample port B (218), the bypass pointer groove B (222) communicates the bypass groove B (225) and the jumper switching port 1B (214), the first switching groove B (223) communicates the jumper switching port 3B (216) and the chromatography switching port 3B (213), the second switching groove B (224) communicates the jumper switching port 2B (215) and the chromatography switching port 2B (212), and the bypass groove B (225) communicates the bypass pointer groove B (222) and the cleaning port B (219);

in step S4, the rotor of the continuous flow chromatography module a (100) is switched by rotation, the switching pointer groove a (121) communicates the chromatography switching port 3A (113) and the sample port a (118), the bypass pointer groove a (122) communicates the bypass groove a (125) and the jumper switching port 2A (115), the first switching groove a (123) communicates the jumper switching port 3A (116) and the chromatography switching port 1A (111), the second switching groove a (124) communicates the jumper switching port 1A (114) and the chromatography switching port 2A (112), and the bypass groove a (125) communicates the bypass pointer groove a (122) and the cleaning port a (119);

in step S5, the rotor of the continuous flow chromatography module B (200) is rotationally switched, the switching pointer groove B (221) communicates the chromatography switching port 2B (212) and the sample port B (218), the bypass pointer groove B (222) communicates the bypass groove B (225) and the jumper switching port 2B (215), the first switching groove B (223) communicates the jumper switching port 1B (214) and the chromatography switching port 1B (211), the second switching groove B (224) communicates the jumper switching port 3B (216) and the chromatography switching port 3B (213), and the bypass groove B (225) communicates the bypass pointer groove B (222) and the cleaning port B (219);

in step S6, the rotor of the continuous flow chromatography module a (100) is switched by rotation, the switching pointer groove a (121) communicates the chromatography switching port 1A (111) and the sample port a (118), the bypass pointer groove a (122) communicates the bypass groove a (125) and the jumper switching port 3A (116), the first switching groove a (123) communicates the jumper switching port 1A (114) and the chromatography switching port 2A (112), the second switching groove a (124) communicates the jumper switching port 2A (115) and the chromatography switching port 3A (113), and the bypass groove a (125) communicates the bypass pointer groove a (122) and the cleaning port a (119);

in step S7, the rotor of the continuous flow chromatography module B (200) is rotated and switched, the switching finger groove B (221) communicates the chromatography switching port 3B (213) and the sample port B (218), the bypass finger groove B (222) communicates the bypass groove B (225) and the jumper switching port 3B (216), the first switching groove B (223) communicates the jumper switching port 2B (215) and the chromatography switching port 2B (212), the second switching groove B (224) communicates the jumper switching port 1B (214) and the chromatography switching port 1B (211), and the bypass groove B (225) communicates the bypass finger groove B (222) and the cleaning port B (219).

13. A continuous flow chromatography system, comprising: including two rotors, stator board (800), jumper board (900), two sample passageways, two washing liquid passageways, a plurality of chromatography post, sample loading pump (530), scavenging pump (630), the rotor is in rotate on stator board (800), the rotor has switching pointer groove, bypass pointer groove, switching groove and bypass groove, stator board (800) have two sets of opening combinations, be equipped with a plurality of jumper wire passageways on jumper board (900), clockwise or anticlockwise rotation in proper order through two rotors realizes the circulation in proper order of a plurality of jumper wire passageways, a plurality of chromatography passageways and switches.

14. The continuous flow chromatography system of claim 13, wherein each jumper channel of the jumper plate (900) is disposed under the stator plate (800) and communicates with two sets of port combinations, each rotor is disposed on the stator plate (800) and communicates with one set of port combinations, each sample channel and each cleaning solution channel are respectively communicated with one set of port combinations, each chromatography channel is respectively communicated with two sets of port combinations, and each chromatography channel is disposed with one chromatography column.

15. The continuous flow chromatography system of claim 13, wherein the port assembly comprises a sample port, a plurality of chromatography switching ports, a plurality of jumper switching ports, and a cleaning port, wherein the chromatography switching ports and the jumper switching ports are circumferentially distributed along the rotation direction, the chromatography switching ports and the jumper switching ports are alternately arranged in equal number, and the sample port and the cleaning port are non-coincident with the distribution circumferences of the chromatography switching ports and the jumper switching ports.

16. The continuous flow chromatography system of claim 15, wherein the chromatography switching port distribution circumference is of the same diameter as the jumper switching port distribution circumference, the sample port is located at a center of the distribution circumference, the purge port is located outside the distribution circumference, and the bypass channel is closed around the periphery of the rotor, or the purge port is located inside the distribution circumference, and the bypass channel is not closed around the center of the rotor.

17. The continuous flow chromatography system of claim 15, wherein each of the sample ports communicates with a sample channel, each of the cleaning ports communicates with a cleaning solution channel, each of the chromatography channels communicates with the chromatography switching ports of the two port combinations at two ends thereof, each of the chromatography switching ports of the set of port combinations communicates with a chromatography channel, each of the jumper channels communicates with the jumper switching ports of the two port combinations at two ends thereof, each of the jumper switching ports of the set of port combinations communicates with a jumper channel, the sample channel is provided with a sample loading pump (530), and the cleaning solution channel is provided with a cleaning pump (630).

18. The continuous flow chromatography system of claim 17, wherein the sample channel and the wash fluid channel are disposed in the stator plate (800), and a plurality of non-interconnected chromatography switching port channels are further disposed in the stator plate (800), each chromatography switching port channel is connected to one chromatography switching port, and the chromatography channels are connected to the chromatography switching ports through the chromatography switching port channels.

19. The continuous flow chromatography system of claim 13, wherein the switching finger slot, the bypass finger slot and the switching slot are disposed on the rotor in a non-communicating manner, the bypass finger slot and the bypass slot are disposed on the rotor in a communicating manner, two ends of the switching finger slot are respectively communicated with the sample port and the chromatography switching port, two ends of the bypass finger slot are respectively communicated with the jumper switching port and the bypass slot, two ends of the switching slot are respectively communicated with the chromatography switching port and the jumper switching port, and the bypass slot is communicated with the bypass finger slot and the cleaning port.

20. The continuous flow chromatography system of claim 13, wherein the jumper plate (900) is provided with a first jumper plate channel (910), a second jumper plate channel (920) and a third jumper plate channel (930) which are not communicated in parallel, the plurality of chromatography columns comprises a first chromatography column (710), a second chromatography column (720) and a third chromatography column (730), the two sets of the through port combinations comprise a through port combination A and a through port combination B, the rotor A (120) communicated with the through port combination A correspondingly is provided with a switching finger slot A (121), a bypass finger slot A (122), a first switching slot A (123), a second switching slot A (124) and a bypass slot A (125), the rotor B (220) communicated with the through port combination B correspondingly is provided with a switching finger slot B (221), a bypass finger slot B (222), a first switching slot B (223), a second switching slot B (224) and a bypass slot B (225), the port combination A is provided with a chromatography switching port 1A (111), a chromatography switching port 2A (112), a chromatography switching port 3A (113), a jumper switching port 1A (114), a jumper switching port 2A (115), a jumper switching port 3A (116), a sample port A (118) and a cleaning port A (119), and the port combination B is provided with a chromatography switching port 1B (211), a chromatography switching port 2B (212), a chromatography switching port 3B (213), a jumper switching port 1B (214), a jumper switching port 2B (215), a jumper switching port 3B (216), a sample port B (218) and a cleaning port B (219).

21. The continuous flow chromatography system of claim 20, wherein the chromatography switching port 1A (111) and the chromatography switching port 1B (211) are connected by a first chromatography channel (410), the chromatography switching port 2A (112) and the chromatography switching port 2B (212) are connected by a second chromatography channel (420), the chromatography switching port 3A (113) and the chromatography switching port 3B (213) are connected by a third chromatography channel (430), and the first chromatography channel (410), the second chromatography channel (420) and the third chromatography channel (430) are respectively provided with the first chromatography column (710), the second chromatography column (720) and the third chromatography column (730) separately.

22. The continuous flow chromatography system of claim 21, wherein the jumper switch port 1a (114) and jumper switch port 1B (214) are connected by a first jumper plate channel (910), the jumper switch port 2a (115) and jumper switch port 2B (215) are connected by a second jumper plate channel (920), the jumper switch port 3a (116) and jumper switch port 3B (216) are connected by a third jumper plate channel (930), the sample port a (118) is connected to sample channel a (510), the sample port B (218) is connected to sample channel B (520), the wash port a (119) is connected to wash solution channel a (610), and the wash port B (219) is connected to wash solution channel B (620).