CN113325063B - Verifying device and method for colloidal gold immunochromatography test paper detection result - Google Patents
Verifying device and method for colloidal gold immunochromatography test paper detection result Download PDFInfo
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- 238000012360 testing method Methods 0.000 title claims abstract description 72
- 238000001514 detection method Methods 0.000 title claims abstract description 66
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000003317 immunochromatography Methods 0.000 title claims description 16
- 239000007788 liquid Substances 0.000 claims abstract description 47
- 239000003480 eluent Substances 0.000 claims abstract description 28
- 238000000605 extraction Methods 0.000 claims abstract description 27
- 238000002955 isolation Methods 0.000 claims abstract description 18
- 238000010828 elution Methods 0.000 claims abstract description 5
- 150000002500 ions Chemical class 0.000 claims description 62
- 238000007789 sealing Methods 0.000 claims description 17
- 238000005070 sampling Methods 0.000 claims description 9
- 238000004458 analytical method Methods 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 4
- 230000002572 peristaltic effect Effects 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000012491 analyte Substances 0.000 claims description 2
- 238000001819 mass spectrum Methods 0.000 description 9
- 239000011521 glass Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 5
- -1 sulfate radical Chemical class 0.000 description 5
- 238000004807 desolvation Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000012795 verification Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000037427 ion transport Effects 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 150000005837 radical ions Chemical class 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013499 data model Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- YPJKMVATUPSWOH-UHFFFAOYSA-N nitrooxidanyl Chemical compound [O][N+]([O-])=O YPJKMVATUPSWOH-UHFFFAOYSA-N 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000003566 sealing material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention provides a device and a method for verifying a detection result of colloidal gold immunochromatographic test paper, wherein the device for verifying the detection result of the colloidal gold immunochromatographic test paper comprises an ion source and a mass spectrometer; the isolation unit is used for forming a liquid channel isolated from the outside, and the liquid channel is used for accommodating the detection line of the test paper; the elution unit is used for providing eluent and sending the eluent into the liquid channel formed by the isolation unit; the extraction unit is used for containing the extraction liquid and receiving the eluent discharged from the isolation unit; and conveying the object to be detected in the extraction unit to the ion source. The invention has the advantages of automation and the like.
Description
Technical Field
The invention relates to colloidal gold immunochromatography test paper detection, in particular to a device and a method for verifying a detection result of colloidal gold immunochromatography test paper.
Background
The colloidal gold immunochromatography technology mainly utilizes the characteristic that gold particles have high electron density, when a large amount of the markers are gathered at corresponding ligands, red or pink spots appear, and qualitative detection can be realized by visually observing the color development strips on the test paper and comparing with a standard color comparison card. The quantitative analysis can be realized by applying the methods of measuring the photoresistor, measuring the reflected light intensity by the reflective optical fiber sensor, acquiring the image by the image sensor and the like to the colloidal gold immunochromatography technology. The existing quantitative detector is used for correlating the ratio T/C of the signal values of the detection line and the quality control line with the sample concentration value to form a curve relation diagram of the signal value and the sample concentration value, so as to calculate the actual concentration of the sample.
The colloidal gold immunochromatography technology is affected and restricted by various factors in various links such as preparation, preservation, analysis and the like. For example, the uniformity and dispersity of the colloidal gold particles can affect the stability and repeatability of the test, if the gold particles are irregular in shape or nonuniform in particle size, the colloidal gold labels are easy to dissociate and precipitate, so that incomplete gold label diffusion and over-deep bottom color of a reaction zone are generated; colloidal gold is of poor quality and the colloidal gold conjugate cannot be rapidly and completely dissociated from the glass fiber. Therefore, in some cases, the measurement process of the colloidal gold immunochromatography may have missed detection or failed detection, such as false positive and false negative, and the accuracy is limited by using the colloidal gold immunochromatography test paper for quantitative analysis.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides a verifying device for the detection result of colloidal gold immunochromatography test paper.
The invention aims at realizing the following technical scheme:
the device for verifying the detection result of the colloidal gold immunochromatographic test paper comprises an ion source and a mass spectrometer; the verifying device of the colloidal gold immunochromatography test paper detection result further comprises:
the isolating unit is used for forming a liquid channel isolated from the outside, and the liquid channel is used for accommodating the detection line of the test paper;
The elution unit is used for providing eluent and sending the eluent into the liquid channel formed by the isolation unit;
An extraction unit for containing an extraction liquid and receiving the eluate discharged from the isolation unit; and conveying the extract of the to-be-detected object in the extraction unit to the ion source.
The invention further aims at providing a method for verifying the detection result of the colloidal gold immunochromatographic test paper by using the verifying device, and the method is realized by the following technical scheme:
the method for verifying the detection result of the colloidal gold immunochromatographic test paper comprises the following steps of:
the detection line of the colloidal gold immunochromatographic test paper is arranged in a liquid channel isolated from the outside;
eluting the object to be detected on the detection line in the channel by using eluent;
The object to be detected enters an extraction unit, the object to be detected is extracted, and the extract enters an ion source;
The extract is ionized and sent to a mass spectrometer for analysis;
and verifying the detection result of the test paper by using the output result of the mass spectrometer.
Compared with the prior art, the invention has the following beneficial effects:
1. the method combines the colloidal gold immunochromatography test paper with the mass spectrum, and simultaneously plays the advantages of immunological detection technology and mass spectrum technology;
The sample can be quickly and primarily screened by the colloidal gold immunochromatography test paper, and mass spectrum verification can be carried out on positive or weak positive or uncertain samples to determine specific content;
2. the device can realize non-contact operation, realizes the processes of elution, extraction, transfer and the like of the object to be detected through a simple device structure, and has simple operation and low cost;
the extraction process is carried out in a closed container, and the introduced liquid has a disturbing effect and is completely extracted;
3. The data model of the relation between the test paper color development and the sample concentration can be optimized through the comparison of the methods; through the verification of multiple groups of parallel tests of the immunochromatography test paper, the mass spectrum parameters can be optimized, and the optimal detection parameter conditions of the mass spectrum can be obtained, so that the sample detection accuracy of the two methods is improved.
Drawings
The present disclosure will become more readily understood with reference to the accompanying drawings. As will be readily appreciated by those skilled in the art: the drawings are only for illustrating the technical scheme of the present invention and are not intended to limit the scope of the present invention. In the figure:
FIG. 1 is a schematic structural diagram of a verifying device for detecting results of colloidal gold immunochromatographic test paper according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of an isolation unit according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of the structure of an extraction unit according to an embodiment of the invention;
Fig. 4 is a schematic structural diagram of an ion sampling apparatus according to an embodiment of the present invention;
FIG. 5 is a left side view of a body according to an embodiment of the present invention;
Fig. 6 is a right side view of a body according to an embodiment of the present invention.
Detailed Description
Figures 1-6 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. For the purpose of explaining the technical solution of the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art will appreciate variations or alternatives derived from these embodiments that fall within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the invention is not limited to the following alternative embodiments, but only by the claims and their equivalents.
Example 1:
Fig. 1 schematically shows a structural diagram of a device for verifying a detection result of a colloidal gold immunochromatographic test strip according to embodiment 1 of the present invention, as shown in fig. 1, the device for verifying a detection result of a colloidal gold immunochromatographic test strip includes:
Ion sources, which are state of the art; the isolation unit is used for accommodating the detection line of the test paper; the elution unit is used for providing eluent and sending the eluent into the isolation unit so as to elute the object to be detected on the detection line in the isolation unit; an extraction unit for containing an extraction liquid and receiving the eluate discharged from the isolation unit; and conveying the extract of the to-be-detected object in the extraction unit to the ion source.
In order to eliminate interference of other parts of the test paper, the isolation unit is further used for cutting the part of the test paper with the detection line.
In order to conveniently form the isolation unit, further, as shown in fig. 2, the isolation unit includes:
A first portion 11, the first portion 11 having a first groove 111, the first groove 111 being provided with seals 112 on both sides thereof; the test strip 3 is adapted to span the first recess 111 and is located on the upper side of the seal 112, and the test line 31 of the test strip 3 is located in the first recess 111; a second portion 12, the underside 121 of the second portion 12 being in contact with the seal 112 to effect a seal.
In order to cut the test paper 3 during the formation of the separation unit such that only the detection wire 31 enters the separation unit, further, the lower side of the second portion 12 has a second groove 122, both sides of the second groove 122 being used to cut the test paper 3 on the upper side of the sealing member 112; the eluent provided by the eluting unit flows through the liquid channel isolated from the outside formed by the first and second grooves 111 and 122.
In order to extract the analyte eluted from the test strip 3 detection line 31, further, as shown in fig. 3, the extraction unit includes:
A container 20 and a first delivery pipe, one end of which is communicated with the outlet of the liquid channel, and the other end of which extends into the container 20 and is positioned at the bottom of the container 20; a second delivery tube and valve 24, one end of the second delivery tube extends into the container 20, and the other end is communicated with the ion source 4; the bottom end of the second conveying pipe extending into the container 20 is higher than the bottom end of the first conveying pipe extending into the container 20; the valve 24 is arranged on the second transfer pipe.
For delivering the extract to the ion source, further, the extraction unit further comprises:
and the peristaltic pump is arranged on the second conveying pipe.
The mass spectrometer comprises a mass analysis instrument and an ion sampling device;
Fig. 4 schematically shows a block diagram of an ion sampling apparatus according to an embodiment of the present invention, as shown in fig. 4, the ion sampling apparatus includes:
an ion funnel 61, the ion funnel 61 including a plurality of electrodes having through holes, the through holes having a diameter gradually smaller along an ion transmission direction; the body 41, as shown in fig. 5-6, at least two straight channels 81 penetrating the body 41 are provided in the body 41, the straight channels 81 are distributed around the central axis of the body 41, and the distance between any two straight channels 81 gradually increases from the ion incident end 411 to the ion emergent end 412 of the body 41; a heating unit and a temperature measuring unit, wherein the heating unit is arranged in the body 41, and the temperature measuring unit is arranged in the body 41; a capillary 51, the capillary 51 is disposed in the linear passage 81, and ions exiting from the capillary 51 sequentially pass through the respective through holes of the ion funnel 61.
In order to improve the heating uniformity in the respective capillaries, further, the linear passage 81 is provided around the heating unit.
In order to improve ion transport efficiency and desolvation ability, further, the straight passage 81 and the through hole satisfy the following relationship:
Δd 1 is the difference between the maximum distance and the minimum distance between the straight passage 81 and the central axis of the body 41, Δd 2 is the difference between the maximum diameter and the minimum diameter of the through hole, L 2 is the distance between the through hole having the maximum diameter and the through hole having the minimum diameter, and L 1 is the length of the capillary 51 projected on the central axis of the body 41.
In order to improve ion transport efficiency and desolvation capacity, further, the straight channel 81 satisfies:。
To increase the desolventizing capacity, further, the capillary tube 51 is wholly or partially within the linear passage 81.
In order to reduce the processing difficulty, further, a heating unit is disposed in the first blind hole 71 of the body 41, a temperature measuring unit is disposed in the second blind hole 91 of the body 41, and openings of the first blind hole 71 and the second blind hole 91 are located at the ion exit end 412 of the body 41.
The method for verifying the detection result of the colloidal gold immunochromatographic test paper, namely the working method of the verification device of the embodiment, comprises the following steps:
The detection line 31 of the colloidal gold immunochromatographic test paper 3 is arranged in a liquid channel isolated from the outside; eluting the object to be detected on the detection line 31 in the channel by using eluent; the object to be detected enters an extraction unit, the object to be detected is extracted, and the extract enters an ion source 4; the extract is ionized and sent to a mass spectrometer for analysis; and verifying the detection result of the test paper by using the output result of the mass spectrometer.
In order to form a liquid channel which is isolated from the outside and is suitable for the eluent to pass through, the liquid channel is further formed in a way that:
The first part 11 has a first groove 111, and seals 112 are provided on both sides of the first groove 111; the test strip 3 is adapted to span the first recess 111 and is located on the upper side of the seal 112, and the test line 31 of the test strip 3 is located in the first recess 111; the underside 121 of the second part 12 is in contact with the seal 112, and a liquid passage isolated from the outside is formed between the underside of the second part 12 and the first recess 111.
In order to complete the cutting of the test paper 3 during the formation of the channel such that the test wire 31 is in the channel, further, the lower side of the second portion 12 has a second groove 122, and both sides of the second groove 122 are used for cutting the test paper 3 on the upper side of the sealing member 112 and keeping sealing with the sealing member 112; the eluting unit provides an eluent flow through the liquid channel formed by the first and second grooves 111, 122.
In order to extract the object to be detected entering the container, further, the extraction mode of the object to be detected is as follows:
Closing the output of the container 20, enabling eluent containing the object to be detected to enter the inner bottom of the container 20, enabling the liquid level in the container 20 to rise, compressing the gas above the liquid level, and extracting the object to be detected by the extraction liquid in the container 20; the output of the vessel 20 is opened and the extract is discharged from the vessel 20 and fed to the ion source 4.
Example 2:
the colloidal gold immunochromatographic test paper according to embodiment 1 of the present invention is applied to a device and a method for verifying the detection result.
In this application example, as shown in fig. 2, in the isolation unit, the first portion 11 has a first groove 111 in a semi-cylindrical shape with an opening facing upward, and sealing members 112 are provided on both sides of the first groove 111; the colloidal gold immunochromatographic test paper 3 is suitable for crossing the first groove 111 and is positioned on the upper side of the sealing piece 112, the distance between the two sealing pieces 112 is 2-10mm plus the width of the detection line 31, and the detection line 31 is positioned at the central position between the two sealing pieces 112; the lower side of the second part 12 is provided with a second semi-cylindrical groove 122 with a downward opening, and two sides of the second groove 122 are blade-shaped and are used for cutting the test paper 3 positioned on the upper side of the sealing piece 112;
the eluent provided by the eluting unit is an aqueous solution, a salt solution, an organic solution or a mixed solution containing acid radical ions, wherein the acid radical ions comprise sulfate radical, hydrochloride radical, nitrate radical, carbonate radical, acetate radical or oxalate radical; eluent flows through the first groove 111 and the second groove 122 to form a hollow cylindrical liquid channel;
As shown in fig. 3, the extraction unit includes:
The device comprises a container 20 and a first conveying pipe, wherein the first conveying pipe comprises a first pipeline 27 and a first glass pipe 21, an interface 22 is arranged between the first pipeline 27 and the first glass pipe 21, one end of the first pipeline 27 is communicated with an outlet of the liquid channel, and one end of the first glass pipe 21 extends into the liquid level in the container 20 and is positioned at the bottom in the container 20; the container 20 has a capacity of 1 mL-10 mL, and is internally provided with an extract 26 with a fixed volume (0.5 mL-8 mL), wherein the extract 26 comprises acetone, methanol, ethanol, dichloromethane, acetonitrile or ethyl acetate; the eluent 28 is an acidic aqueous solution, the extract 26 has a density greater than the eluent 28, and the extract 26 and the eluent 28 are immiscible;
A second conveying pipe and a valve 24, wherein the second conveying pipe comprises a second pipeline 25 and a second glass pipe 23, one end of the second glass pipe 23 extends into the container 20, and one end of the second pipeline 25 is communicated with the ion source 4; the position of the bottom end of the second glass tube 23 extending into the container 20 is higher than the position of the bottom end of the first glass tube 21 extending into the container 20; the valve 24 is arranged at the joint of the second pipeline 25 and the second glass 23 pipe;
in the ion sampling device, as shown in fig. 4, a body 41 and an ion funnel 61 are disposed in a vacuum chamber 32;
As shown in fig. 5-6, the ion exit end 412 of the body 41 has a first blind hole 71 and a second blind hole 91, the central axis of the body 41 is collinear with the central axis of the first blind hole 71, the body 41 also has a plurality of (e.g. 2, 4,7 or 8, etc.) linear channels 81 (which are uniformly distributed around the first blind hole 71) penetrating the body 41, and the distance between any two linear channels 81 is gradually increased from the ion entrance end 411 to the ion exit end 412 of the body 41; an electric heating unit is arranged in the first blind hole 71, and a temperature measuring unit is arranged in the second blind hole 91;
As shown in fig. 1, the inner diameter of the capillary tube 51 is 0.3-0.4mm, the capillary tube 51 is arranged in each linear channel 81, sealing materials are arranged between the capillary tube 51 and the linear channels 81, two ends of the capillary tube 51 extend out of the linear channels 81, one end of the capillary tube is positioned out of the vacuum cavity 32, and the other end extends into the vacuum cavity 32 out of the linear channels 81 and extends into the ion funnel 61;
the ion funnel 61 includes a plurality of (e.g., 30) electrodes having through holes with an electrode pitch of 1.2mm, the through holes having a diameter gradually decreasing along the ion transmission direction;
the straight passage 81 satisfies the condition Δd 1 is the difference between the maximum distance and the minimum distance between the linear passage 81 and the central axis of the body 41, and L 1 is the length of the capillary 51 projected on the central axis of the body 41;
the straight passage 81 and the through hole satisfy the following relationship:
Δd 1 is the difference between the maximum distance and the minimum distance between the straight passage 81 and the central axis of the body 41, Δd 2 is the difference between the maximum diameter and the minimum diameter of the through hole, L 2 is the distance between the through hole having the maximum diameter and the through hole having the minimum diameter, and L 1 is the length of the capillary 51 projected on the central axis of the body 41.
In this embodiment, specific parameters are: the tail end portion of the capillary 51 is within a radial distance of 4mm to 10mm from the electrode in the nearest ion funnel 61;
in the ion funnel 61, the electrode pitch is 1.2mm, the diameter of the through hole is gradually reduced along the ion transmission direction, and the diameter difference of the through holes between adjacent electrodes is 1mm;
Δd is the difference between the maximum and minimum radii of the through holes of each electrode in the ion funnel 61, and L is the distance between the first electrode and the last electrode in the ion funnel 61; the maximum diameter of the through hole is 60mm and the minimum diameter is 1.5mm, namely the difference between the maximum diameter and the minimum diameter is 58.5mm; the distance between the maximum diameter and the minimum diameter of the through holes is 76mm.
The included angle between the capillary tube 51 in the straight passage 81 and the central axis of the body 41 is 1-2.5 degrees, the difference between the maximum distance and the minimum distance between the straight passage 81 and the central axis of the body 41 is 2.5-5 mm, and the projection length of the capillary tube 51 on the central axis of the body 41 is 4.5-6 mm.
The method for verifying the detection result of the colloidal gold immunochromatographic test paper of the embodiment of the invention, namely the working method of the device for verifying the detection result of the colloidal gold immunochromatographic test paper of the embodiment of the invention, comprises the following steps:
The test paper 3 with the object to be tested is paved on the upper side of the first part 11, wherein a detection line 31 of the test paper 3 is positioned at the central position between the two sealing elements 112, and the distance between the detection line 31 and the sealing elements 112 is 1-5mm;
the second part 12 contacts the sealing member 112 from top to bottom to maintain sealing, and during the contact, the two blade-like structures cut the test paper 3 on the upper side of the sealing member 112, and the first groove 111 and the second groove 122 are combined into a liquid channel, so that the detection line 31 is located in the liquid channel;
Closing valve 24 on the second transfer tube, the eluent entering the liquid channel;
The object to be detected on the detection line 31 enters the first conveying pipe along with the eluent, and then enters the bottom in the container 20; the liquid level in the container 20 rises, and the gas above the liquid level is compressed;
The eluent 28 moves up in the container 20, and the object to be detected is extracted by the extracting liquid 26 in the upward movement process;
after the volume of the eluent 28 entering the container 20 is 0.5-5mL, stopping feeding the eluent 28 in the liquid channel, and opening the valve 24;
the extract is discharged from the container 20 through a second conveying pipe, is sent to the ion source 4 for ionization and is finally sent to a mass spectrometer for analysis;
the mass spectrum detection result is used as a standard result, a relation model of the color development signal value and the sample concentration value of the test paper is corrected, and the quantitative accuracy of the colloidal gold immunochromatography test paper on an actual sample is improved;
in addition, the optimal detection parameter condition of the mass spectrum on the specific sample can be obtained by adjusting the detection parameters of the mass spectrum by taking the color development result of the colloidal gold as a reference through multiple groups of parallel sample injection.
In this embodiment, since the ion sampling device different from the prior art is adopted, the technical effects achieved are as follows:
1. the subsequent analysis has high sensitivity and little pollution of an ion funnel;
compared with a single capillary tube without an inclined angle, the ion flux is increased, and compared with a single capillary tube with an off-axis, the ion loss is less, the signal intensity of transmitted ions is improved, the subsequent analysis sensitivity is correspondingly improved, and the pollution to the electrode plate of the ion funnel at the subsequent stage is reduced;
2. The desolvation capability is strong;
Compared with the traditional non-inclined multi-capillary sample injection, the method has the advantages that the pollution resistance is greatly improved, and the desolvation capacity is enhanced;
3. the maintenance is convenient;
In view of the fact that the traditional multi-capillary sample injection is integrally processed, disassembly and cleaning are inconvenient, the device is easy to clean and convenient to replace;
4. the sensitivity of mass spectrum detection is high;
When the rectilinear channel and the ion funnel are defined, e.g 、Neutral ions entering the funnel from the capillary tube in the body 41 are well inhibited, detection pollution to a later-stage mass analyzer is avoided, and detection sensitivity of the mass spectrometer is further improved.
Example 3:
The application example of the device and method for verifying the detection result of the colloidal gold immunochromatographic test strip according to embodiment 1 of the present invention is different from embodiment 2 in that:
1. the peristaltic pump is arranged on the second conveying pipe, and the peristaltic pump is used for conveying the extract in the container to the ion source;
2. The capillary is completely positioned in the straight channel;
3. A drift tube is disposed between the body and the ion funnel, and a portion of the body extends into the drift tube.
Claims (8)
1. The device for verifying the detection result of the colloidal gold immunochromatographic test paper comprises an ion source and a mass spectrometer; the device is characterized in that the verifying device of the colloidal gold immunochromatography test paper detection result further comprises:
the isolating unit is used for forming a liquid channel isolated from the outside, and the liquid channel is used for accommodating the detection line of the test paper;
The elution unit is used for providing eluent and sending the eluent into the liquid channel formed by the isolation unit;
An extraction unit for containing an extraction liquid and receiving the eluate discharged from the isolation unit; the extract of the object to be detected in the extraction unit is sent to the ion source;
The mass spectrometer comprises an ion sampling device, wherein the ion sampling device comprises an ion funnel, the ion funnel comprises a plurality of electrodes with through holes, and the diameters of the through holes gradually become smaller along the ion transmission direction; the ion sampling device further comprises:
The ion source comprises a body, wherein the body is internally provided with at least two linear channels penetrating through the body, the linear channels are distributed around the central axis of the body, and the distance between any two linear channels is gradually increased from the ion incident end to the ion emergent end of the body;
the heating unit is arranged in the body, and the temperature measuring unit is arranged in the body;
the capillary tube is arranged in the linear channel, and ions emitted from the capillary tube sequentially pass through all through holes of the ion funnel;
The straight-line channel and the through hole satisfy the following relation:
Δd 1 is the difference between the maximum distance and the minimum distance between the straight line channel and the central axis of the body, L 1 is the length of the capillary projected on the central axis of the body, Δd 2 is the difference between the maximum diameter and the minimum diameter of the through hole, and L 2 is the distance between the through hole having the maximum diameter to the through hole having the minimum diameter.
2. The apparatus for verifying the detection result of colloidal gold immunochromatographic test strip according to claim 1, in which the isolation unit is used for cutting a portion of the test strip having a detection line.
3. The apparatus for verifying the detection result of colloidal gold immunochromatographic test strip according to claim 2, wherein the isolation unit comprises:
a first portion having a first groove, both sides of which are provided with sealing members; the test paper spans the first groove and is positioned on the upper side of the sealing element, and a detection line of the test paper is positioned in the first groove;
a second portion, an underside of the second portion being in contact with the seal.
4. A device for verifying the detection result of colloidal gold immunochromatographic test strip according to claim 3, in which the lower side of the second portion has a second groove, both sides of which are used for cutting the test strip on the upper side of the sealing member; the eluent provided by the eluting unit flows through a liquid channel which is isolated from the outside and is formed by the first groove and the second groove.
5. The apparatus for verifying the detection result of colloidal gold immunochromatographic test strip as defined in claim 4, wherein the extraction unit comprises:
The liquid channel is communicated with the liquid outlet through the liquid inlet pipe, and the liquid channel is communicated with the liquid outlet through the liquid outlet pipe;
One end of the second conveying pipe extends into the container, and the other end of the second conveying pipe is communicated with the ion source; the bottom end of the second conveying pipe extending into the container is higher than the bottom end of the first conveying pipe extending into the container; the valve is arranged on the second conveying pipe.
6. The apparatus for verifying the detection result of colloidal gold immunochromatographic test strip as defined in claim 5, wherein the extraction unit further comprises:
and the peristaltic pump is arranged on the second conveying pipe.
7. The method for verifying the detection result of the colloidal gold immunochromatographic test strip for verifying the device according to any one of claims 1 to 6, wherein the method for verifying the detection result of the colloidal gold immunochromatographic test strip is as follows:
the detection line of the colloidal gold immunochromatographic test paper is arranged in a liquid channel isolated from the outside;
Eluting the object to be detected on the detection line in the liquid channel by using eluent;
The object to be detected enters an extraction unit, the object to be detected is extracted, and the extract enters an ion source;
The extract is ionized and sent to a mass spectrometer for analysis;
and verifying the detection result of the test paper by using the output result of the mass spectrometer.
8. The method for verifying the detection result of the colloidal gold immunochromatographic test strip according to claim 7, wherein the extraction method of the analyte is as follows:
Closing the output of the container, enabling eluent containing the object to be detected to enter the inner bottom of the container, enabling the liquid level in the container to rise, compressing gas on the upper side of the liquid level, and extracting the object to be detected by the extraction liquid in the container;
the output of the vessel is opened and the extract is discharged from the vessel and fed to an ion source.
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