CN113324965A - Portable detection device and method based on fluorescent test paper tape - Google Patents
Portable detection device and method based on fluorescent test paper tape Download PDFInfo
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- CN113324965A CN113324965A CN202110751551.1A CN202110751551A CN113324965A CN 113324965 A CN113324965 A CN 113324965A CN 202110751551 A CN202110751551 A CN 202110751551A CN 113324965 A CN113324965 A CN 113324965A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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Abstract
The invention belongs to the technical field of in-vitro diagnosis, analysis and detection equipment, and discloses a portable detection device and a method based on a fluorescent test paper tape, aiming at the problems that multiple indexes cannot be detected simultaneously in the prior art and the detection efficiency and the detection precision are low due to the structural design of the conventional portable detection device, wherein the portable detection device comprises: lens hood and reagent strip, the lens hood is including dismantling left lens hood and the right lens hood of connecting, be provided with the current inner chamber of confession light path between left side lens hood and the right lens hood, the intracavity is provided with the baffle of a plurality of vertical directions, the baffle separates the inner chamber for a plurality of passageways, be provided with the condensing element who is used for the spotlight between the baffle, all the detachable detection device who is connected with the detection light wavelength in every passageway top, the top of lens hood is provided with the control panel, control panel and detection device electric connection, be provided with the excitation chamber that link up with the inner chamber in the lens hood, the lower extreme in excitation chamber is located condensing element's top, it is provided with the excitation light source to excite the intracavity.
Description
Technical Field
The invention belongs to the technical field of in-vitro diagnosis, analysis and detection equipment, and particularly relates to a portable detection device and method based on a fluorescent test paper tape.
Background
A common diagnosis carrier in the detection method of the paper-based detection reagent strip, a nitrocellulose membrane is common in the material of the paper, and the principle of an Immunochromatography (ICA) detection technology is as follows: a specific antibody is firstly fixed in a certain zone of a nitrocellulose membrane, after one end of the dried nitrocellulose membrane is immersed in a sample (urine or serum), the sample moves forwards along the membrane due to capillary action, when the sample moves to a region where the antibody is fixed, a corresponding antigen in the sample is specifically combined with the antibody, and if the region is stained by immune colloidal gold or immune enzyme, the region can show a certain color or fluorescence property, so that specific immunodiagnosis is realized.
For the detection of the color development area, as for the prior art, only single index detection can be usually performed, when multiple indexes are required to be detected, different detection instruments are required to be replaced for detection, and the detection efficiency is low, so that a method for simultaneously detecting the multiple indexes is not mature; in terms of detection accuracy, some large-sized full-automatic analyzers have relatively high detection accuracy in the prior art, but for some portable detection devices, the detection index value accuracy of the current detection device is not accurate enough, and application scenarios of high-accuracy requirement analysis cannot be met.
Disclosure of Invention
The embodiment of the invention aims to provide a portable detection device and a method based on a fluorescent test paper tape, which solve the problem that in the prior art, only single index detection can be carried out, and multiple indexes cannot be detected simultaneously, so that the detection efficiency is low.
In order to solve the above technical problem, an embodiment of the present invention provides a portable detection device based on a fluorescence test paper tape, including: lens hood and reagent strip, the lens hood is including dismantling left lens hood and the right lens hood of connection, be provided with the current inner chamber of confession light path between left side lens hood and the right lens hood, the baffle of a plurality of vertical directions is provided with in the inner chamber, the baffle separates the inner chamber for a plurality of passageways, be provided with the spotlight element that is used for the spotlight between the baffle, every the passageway top all can be dismantled and be connected with the detection device who detects the light wavelength, the top of lens hood is provided with the control panel, control panel and detection device electric connection, be provided with the excitation chamber that link up with the inner chamber in the lens hood, the lower extreme that excites the chamber is located spotlight element's top, it is provided with the excitation light source to excite the intracavity.
Furthermore, the light-gathering element is formed by splicing a plurality of double convex lenses, each double convex lens corresponds to one channel, and the double convex lenses are horizontally arranged.
Further, the detection device is a photoelectric sensor.
Furthermore, the excitation cavities correspond to the channels one to one, the excitation cavities and the horizontal plane are arranged at a certain angle, the lower ends of the excitation cavities are communicated with the channels to form exit ports, and the exit ports are located above the light condensing elements.
Further, the angle is 30-60 degrees.
Furthermore, a first optical filter is arranged between the excitation light source and the exit port, and the first optical filter is perpendicular to the light emitted by the excitation light source.
Furthermore, a second optical filter in the horizontal direction is arranged between the detection device and the light-gathering element, and the second optical filter is clamped between the partition plates.
Furthermore, the material of the light shield is ABS, PC, PP or POM.
A portable detection method based on a fluorescent reagent strip comprises the following steps:
the method comprises the following steps: arranging a light-gathering element, arranging a groove on a partition plate in the channel, wherein the groove is positioned at the lower end of the excitation cavity, horizontally clamping the light-gathering element between the partition plates, and fixing the light-gathering element by using an adhesive method;
step two: the detection light is generated by an excitation light source in the excitation cavity and is transmitted into the channel through the lower end of the excitation cavity;
step three: condensing light through a light condensing element, and then condensing the light onto the fluorescent lines of the test paper tapes corresponding to the channels;
step four: the fluorescence line generates reflected light, the reflected light is condensed by the condensing element again, and then the reflected light is condensed on the detection device.
Further, before the detection light is transmitted to the channel, the detection light is purified and filtered through a first optical filter; the reflected light is purified and filtered by a second optical filter after passing through the light-gathering element and before reaching the detection device.
Advantageous effects
The invention relates to a portable detection device and a portable detection method based on a fluorescent test paper strip.
Drawings
FIG. 1 is a schematic structural diagram of a portable fluorescent test paper strip-based detection device according to a first embodiment of the present invention;
FIG. 2 is an exploded view of a detection device provided in accordance with a first embodiment of the present invention;
FIG. 3 is a right side sectional view of a detecting unit according to a first embodiment of the present invention;
FIG. 4 is a front sectional view of a detecting unit according to a first embodiment of the present invention;
FIG. 5 is a ray path diagram of a detection device provided in accordance with a first embodiment of the present invention;
fig. 6 is a ray path diagram of a detecting device provided according to a second embodiment of the present invention.
Reference numerals: the device comprises a light shield 10, a left light shield 11, a right light shield 12, an excitation cavity 121, an excitation light source 122, a partition plate 13, a first groove 14, a reagent strip 20, a detection area 21, a test strip 22, a fluorescent line 23, a light-condensing element 30, a detection device 40, a control board 50, a first optical filter 60 and a second optical filter 70.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, embodiments of the present invention will be described in detail below with reference to the accompanying drawings. However, it will be appreciated by those of ordinary skill in the art that numerous technical details are set forth in order to provide a better understanding of the present application in various embodiments of the present invention. However, the technical solution claimed in the present application can be implemented without these technical details and various changes and modifications based on the following embodiments.
Example one
Fig. 1 to fig. 5 are schematic structural diagrams of a immunochromatography-based multi-channel rapid detection device according to an embodiment of the present invention, the detection device can simultaneously perform detection of multiple indexes, the detection efficiency is greatly improved, and the device has a simple structure and is convenient to use.
Referring to fig. 1-4, a schematic structural diagram of a detection device according to an embodiment of the present invention includes a light shield 10, the light shield is divided into a left light shield 11 and a right light shield 12, the left light shield 11 and the right light shield 12 are connected by bolts, the light shield 10 is made of ABS, PC, PP or POM, a space for light path to pass through is disposed between the left light shield 11 and the right light shield 12, a plurality of partition plates 13 are installed in the space, the space is divided into a plurality of channels, each channel corresponds to a detection position, the number of the channels corresponds to a detection area 21 of a reagent strip 20 one by one, and the channels can be set according to actual needs, and no specific setting is made in the present scheme; a first groove 14 in the horizontal direction is formed between the partition plates 13, a light condensing element 30 for condensing light is arranged between the first grooves 14, the light condensing element 30 is formed by splicing a plurality of double convex lenses, the two convex surfaces are distributed up and down, and each lens is arranged in one channel and is perpendicular to the direction of the channel. It can be understood that: the focusing of light rays can be better continued through the biconvex lens; all be provided with detection device 40 that detects light wavelength in the top of every passageway, detection device 40 can dismantle with baffle 13 and be connected, adopts bolted connection in this scheme, and this detection device 40 adopts photoelectric sensor, specifically is: the photoelectric diode, the avalanche photodiode or the photomultiplier and the like carry out the judgment of different index detection by identifying different wavelengths of light; a control panel 50 is arranged above the space between the left light shield 11 and the right light shield 12, the detection device 40 is fixedly mounted on the control panel 50, and the control panel 50 is used for controlling the opening and closing of the detection device 40.
Referring to fig. 2 again, the right light shield 11 is provided with an excitation cavity 111, the excitation cavity 111 is disposed at a certain angle with the horizontal plane, and is inclined to the left and below, the angle of the excitation cavity 111 is in a range of 30-60 degrees, the excitation cavity 111 penetrates the right light shield 11 downward, an excitation light source 112 is disposed in the excitation cavity 111, the excitation light source 112 is selected to match with the reagent strip 20, in this embodiment, an LED lamp with a wavelength of 365nm is adopted, and an exit end of the excitation light source 112 faces to the left and below, so that the emitted light source can be incident to the detection area 21 of the reagent strip 20. A reagent strip 20 is arranged below the light shield 10, a test paper tape 22 is arranged in the reagent strip 20, and fluorescent lines 23 of the test paper tape 22 are located in a detection area 21 of the reagent strip 20, wherein each fluorescent line 23 in the detection area 21 corresponds to each channel of the light shield 10, and each channel correspondingly performs index detection.
The detection process of the invention is as follows:
referring to fig. 1-5, the left light shield 11 and the right light shield 12 are fixed by bolts, the detection area 21 of the reagent strip 20 is aligned with each channel formed by the light shield 10, then the excitation light sources 112 and the detection device 40 are turned on, each excitation light source 112 simultaneously emits detection light, and the detection light travels to each angle of the space, so that the light is condensed by the light condensing element 30, so that the light beam is concentrated on the fluorescent line 23 of the detection area 21 of the reagent strip 20, and then the fluorescent line 23 excites different reflected lights to be condensed by the light condensing element 30 again, and finally the light is condensed to the detection device 40 for detection.
Example two
Referring to fig. 6, the present embodiment has a structure similar to that of the first embodiment, so that the same parts are not required to be changed, and the present embodiment is different in that a first optical filter 60 is disposed in an excitation chamber 112, the first optical filter 60 is disposed between an excitation light source 122 and an excitation chamber 121, a second optical filter 70 is disposed between the detecting device 40 and a light-condensing element 30, the second optical filter 70 is disposed in parallel with the light-condensing element 30, the second optical filter 70 is clamped on a partition plate 13, and both the first optical filter 60 and the second optical filter 70 are used for improving the purity of the wavelength of the light source.
The foregoing is merely an example of the present invention, and common general knowledge in the field of known specific structures and characteristics is not described herein in any greater extent than that known in the art at the filing date or prior to the priority date of the application, so that those skilled in the art can now appreciate that all of the above-described techniques in this field and have the ability to apply routine experimentation before this date can be combined with one or more of the present teachings to complete and implement the present invention, and that certain typical known structures or known methods do not pose any impediments to the implementation of the present invention by those skilled in the art. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. A portable fluorescent test paper-based detection device, comprising: lens hood and reagent strip, the lens hood is including dismantling left lens hood and the right lens hood of connection, be provided with the current inner chamber of confession light path between left side lens hood and the right lens hood, the baffle of a plurality of vertical directions is provided with in the inner chamber, the baffle separates the inner chamber for a plurality of passageways, be provided with the spotlight element that is used for the spotlight between the baffle, every the passageway top all can be dismantled and be connected with the detection device who detects the light wavelength, the top of lens hood is provided with the control panel, control panel and detection device electric connection, be provided with the excitation chamber that link up with the inner chamber in the lens hood, the lower extreme that excites the chamber is located spotlight element's top, it is provided with the excitation light source to excite the intracavity.
2. A portable fluorescent test paper strip-based detection device as claimed in claim 1, wherein: the light-gathering element is formed by splicing a plurality of double convex lenses, each double convex lens corresponds to one channel, and the double convex lenses are horizontally arranged.
3. The immunochromatography multichannel hybrid high-precision rapid detection device according to claim 2, characterized in that: the detection device is a photoelectric sensor.
4. A portable fluorescent test paper strip-based detection device as claimed in claim 3, wherein: the light-gathering device is characterized in that the excitation cavities correspond to the channels one to one, the excitation cavities and the horizontal plane are arranged at a certain angle, the lower ends of the excitation cavities are communicated with the channels to form exit ports, and the exit ports are located above the light-gathering elements.
5. The portable fluorescent test paper strip-based detection device as claimed in claim 4, wherein: the angle is 30-60 degrees.
6. A portable fluorescent test paper strip-based detection device as claimed in claim 1, wherein: a first optical filter is arranged between the excitation light source and the exit port, and the first optical filter is perpendicular to light rays emitted by the excitation light source.
7. The portable fluorescent test paper strip-based detection device as claimed in claim 5, wherein: and a second optical filter in the horizontal direction is arranged between the detection device and the light-gathering element, and the second optical filter is clamped between the partition plates.
8. The portable fluorescent test paper strip-based detection device as claimed in claim 6, wherein: the material of the light shield is ABS, PC, PP or POM.
9. A portable detection method based on a fluorescent reagent strip is characterized by comprising the following steps:
the method comprises the following steps: arranging a light-gathering element, arranging a groove on a partition plate in the channel, wherein the groove is positioned at the lower end of the excitation cavity, horizontally clamping the light-gathering element between the partition plates, and fixing the light-gathering element by using an adhesive method;
step two: the detection light is generated by an excitation light source in the excitation cavity and is transmitted into the channel through the lower end of the excitation cavity;
step three: condensing light through a light condensing element, and then condensing the light onto the fluorescent lines of the test paper tapes corresponding to the channels;
step four: the fluorescence line generates reflected light, the reflected light is condensed by the condensing element again, and then the reflected light is condensed on the detection device.
10. The portable fluorescent reagent strip-based detection method according to claim 9, wherein: before the detection light is transmitted to the channel, the detection light is purified and filtered through a first optical filter; and the reflected light is purified and filtered by a second optical filter after passing through the light-gathering element and before reaching the detection device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110751551.1A CN113324965A (en) | 2021-07-02 | 2021-07-02 | Portable detection device and method based on fluorescent test paper tape |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110751551.1A CN113324965A (en) | 2021-07-02 | 2021-07-02 | Portable detection device and method based on fluorescent test paper tape |
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| CN113324965A true CN113324965A (en) | 2021-08-31 |
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| CN202110751551.1A Pending CN113324965A (en) | 2021-07-02 | 2021-07-02 | Portable detection device and method based on fluorescent test paper tape |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114047170A (en) * | 2021-11-27 | 2022-02-15 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN114965995A (en) * | 2022-05-20 | 2022-08-30 | 北京与子成科技有限公司 | Immunochromatography analysis detection system and method |
-
2021
- 2021-07-02 CN CN202110751551.1A patent/CN113324965A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114047170A (en) * | 2021-11-27 | 2022-02-15 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN114047170B (en) * | 2021-11-27 | 2022-08-16 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN114965995A (en) * | 2022-05-20 | 2022-08-30 | 北京与子成科技有限公司 | Immunochromatography analysis detection system and method |
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