CN113249196A - Thermal expansion and cold contraction type integrated reaction tube - Google Patents
Thermal expansion and cold contraction type integrated reaction tube Download PDFInfo
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- CN113249196A CN113249196A CN202110490207.1A CN202110490207A CN113249196A CN 113249196 A CN113249196 A CN 113249196A CN 202110490207 A CN202110490207 A CN 202110490207A CN 113249196 A CN113249196 A CN 113249196A
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- tube
- sampling
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- capillary
- sampling tube
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- 230000008602 contraction Effects 0.000 title claims abstract description 14
- 238000005070 sampling Methods 0.000 claims abstract description 82
- 230000003321 amplification Effects 0.000 claims abstract description 43
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 43
- 238000001816 cooling Methods 0.000 claims description 8
- 238000002844 melting Methods 0.000 claims description 5
- 230000008018 melting Effects 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 238000002834 transmittance Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000004907 flux Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- -1 bacteria Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention relates to the technical field of medical instruments, in particular to a thermal expansion and cold contraction type integrated reaction tube, which comprises a sampling cap, a sampling tube, a capillary tube, a wax film and an amplification tube, wherein the sampling cap is arranged at the top of the sampling tube; the bottom of the sampling tube is provided with an amplification tube, and the sampling tube is detachably connected with the amplification tube; sampling tube grooves are symmetrically formed in the bottom of the sampling tube by taking the capillary tube as an axis; one end of the capillary tube is embedded at the bottom of the sampling tube, and the end part of the capillary tube is higher than the bottom plane of the sampling tube; the other end of the capillary tube extends to the amplification tube through the bottom of the sampling tube; the wax film is provided at the end of a capillary extending to the end of the amplification tube. The invention is an integrated sample adding tube, which can carry out subsequent experiments in the tube only by one-step sample adding without subsequent manual operation, and the results can be obtained in one step.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a thermal expansion and cold contraction type integrated reaction tube.
Background
At present, the screening of a large amount of nucleic acids such as bacteria, viruses and the like is mainly completed by pure manual or manual + semi-mechanical operation in a laboratory at the present stage, one-step sample injection detection cannot be realized, and the screening cannot be carried out in a PCR laboratory and on-site screening cannot be carried out. Although the existing mobile PCR laboratory like a mobile shelter can be placed on site, the occupied area is large, the laboratory is not flexible, the manufacturing cost is high, the laboratory can be completed by pure manual or manual plus semi-mechanical operation, and one-step sample injection detection cannot be realized. The existing full-automatic nucleic acid detecting instrument has a complicated internal mechanical structure, so that the instrument has small volume but low flux, can not screen a large number of samples on site, increases the flux and has large volume correspondingly.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a thermal expansion and cold contraction type integrated reaction tube.
The specific technical scheme of the invention is as follows:
the invention provides a heat expansion and cold contraction type integrated reaction tube, which comprises a sampling cap, a sampling tube, a capillary tube, a wax film and an amplification tube,
the sampling cap is arranged at the top of the sampling tube;
the bottom of the sampling tube is provided with an amplification tube, and the sampling tube is detachably connected with the amplification tube;
sampling tube grooves are symmetrically formed in the bottom of the sampling tube by taking the capillary tube as an axis;
one end of the capillary tube is embedded at the bottom of the sampling tube, and the end part of the capillary tube is higher than the bottom plane of the sampling tube; the other end of the capillary tube extends to the amplification tube through the bottom of the sampling tube;
the wax film is provided at the end of a capillary extending to the end of the amplification chip
Preferably, the bottom cylindrical surface of the sampling tube is provided with sampling tube buckling bulges, the inner layer of the upper part of the amplification tube is symmetrically provided with amplification tube buckling grooves, and the sampling tube buckling bulges and the amplification tube buckling grooves form a trapezoidal buckling structure; the sampling tube and the inclined plane of the amplification tube are assembled and sealed in an interference manner.
Preferably, the top of the sampling cap is provided with a lifting handle, the outer surface of the sampling cap is provided with convex-concave anti-skidding lines, the inner surface of the sampling cap is provided with threads, and the top of the inner surface of the sampling cap is provided with a sealing ring.
Preferably, the melting point of the wax film is 40 to 95 ℃, and more preferably, the melting point of the wax film is 40 to 60 ℃.
Preferably, the bottom of the amplification tube is designed to be completely transparent, and the light transmittance is 80% or more.
Compared with the prior art, the invention has the beneficial effects that:
the integrated sample adding tube can be used for carrying out subsequent experiments in the tube only by one-step sample adding without subsequent manual operation, and the results are obtained in one step.
Drawings
FIG. 1 is a schematic view of the overall assembly of a thermal expansion and cooling shrinkage type integrated reaction tube according to the present invention;
FIG. 2 is a schematic view of the bottom of a sampling tube in a thermal expansion and cooling shrinkage type integrated reaction tube according to the present invention;
FIG. 3 is a schematic view of the bottom sampling tube and the clamping and buckling of the amplification tube of the thermal expansion and cooling shrinkage type integrated reaction tube according to the present invention;
FIG. 4 is a schematic external view of a thermal expansion and contraction type integrated reaction tube sampling cap according to the present invention;
FIG. 5 is a schematic view of the interior of a thermal expansion and contraction type integrated reaction tube sampling cap according to the present invention;
FIG. 6 is a schematic layout view of a thermal expansion and cooling shrinkage type integrated reaction tube according to the present invention;
FIG. 7 is a schematic view of the heating of the thermal expansion and contraction type integrated reaction tube according to the present invention;
reference numerals:
1. sampling tube, 2, wax film, 3, sampling cap, 5, handle, 6, anti-skid lines, 7, screw thread, 8, sampling tube groove, 9, capillary, 10, amplification tube, 11, sealing ring, 12, sampling tube buckle protrusion, 13, amplification tube buckle groove.
Detailed Description
The invention is further illustrated by the following examples and figures.
The invention provides a thermal expansion and cold contraction type integrated reaction tube, which comprises a sampling cap 3, a sampling tube 1, a capillary tube 9, a wax film 2 and an amplification tube 10, wherein the sampling cap 3 is arranged at the top of the sampling tube 1; the bottom of the sampling tube 1 is provided with an amplification tube 10, and the sampling tube 1 is detachably connected with the amplification tube 10; a sampling tube groove 8 is arranged at the bottom of the sampling tube 1 by taking a capillary tube 9 as an axial center; one end of the capillary 9 is embedded in the bottom of the sampling tube 1, and the end position of the capillary 9 is higher than the bottom plane of the sampling tube 1; the other end of the capillary 9 extends to the amplification tube 10 through the bottom of the sampling tube 2; the wax film 2 is provided at the end of a capillary 9 extending to the end of an amplification chip, and has a melting point of 40 to 95 ℃. A sampling tube buckling bulge 12 is arranged on the outer side of the bottom cylindrical surface of the sampling tube 1, an amplification tube buckling groove 13 is symmetrically arranged on the inner layer of the upper part of the amplification tube 10, and the sampling tube buckling bulge 12 and the amplification tube buckling groove 13 form a trapezoidal buckling structure; the sampling tube 1 is assembled and sealed with the inclined surface of the amplification tube 10 in an interference manner. The top of the sampling cap 3 is provided with a lifting handle 5, the outer surface of the sampling cap 3 is provided with convex-concave anti-skid grains 6, the inner surface of the sampling cap is provided with threads 7, and the top of the inner surface of the sampling cap is provided with a sealing ring 11. The bottom of the amplification tube 10 is designed to be completely transparent, and the light transmittance is more than 80%.
The sampling tube groove can store heavy and large foreign matters such as test paper hanging skins or large talking blocks; the blocking probability of the capillary tube is reduced, and the weight of the reagent tube is reduced; the capillary tube is higher than the bottom surface of the inner cavity of the sampling tube, so that the skin or a large talking block is prevented from covering the capillary hole; the probability of capillary blockage is reduced.
The material of other parts except the O-shaped sealing ring 4 can be plastic materials such as PP, PC, POM, nylon and the like which do not react with the reagent in the reaction tube. The handle on the sampling cap solves the problem of tight installation in the instrument, and the handle is square and is prevented from rolling when being horizontally placed alone.
The working principle of the invention is as follows: the liquid flow is realized by heating, cooling and the like, the arrangement of the reaction tubes in the instrument is shown as 6, the amplification tubes are placed in the heater in the middle of the heater in FIG. 2, and the test tubes are heated in the heater as shown in FIG. 7. Heating the amplification tube to 65 ℃ without limitation at 65 ℃ (melting wax seal) to make the gas in the amplification tube discharge to the sampling tube due to thermal expansion, then cooling to 45 ℃ without limitation at 45 ℃, extracting quantitative liquid (10 microliter) from the sampling tube, and then heating to 65 ℃ to carry out amplification reaction.
The method comprises the following operation steps: after adding a swab or other sample into the reaction tube, the sampling tube cap is screwed on and placed in the instrument. The reaction tube realizes the extraction of liquid through heating and cooling, heats the liquid of the amplification tube again, and the reagent begins to react and finally passes through the detection device to detect signals below.
Conventional technical knowledge in the art can be used for the details which are not described in the present invention.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and are not limited. Although the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (5)
1. A thermal expansion and cold contraction type integrated reaction tube is characterized in that the reaction tube comprises a sampling cap, a sampling tube, a capillary tube, a wax film and an amplification tube,
the sampling cap is arranged at the top of the sampling tube;
the bottom of the sampling tube is provided with an amplification tube, and the sampling tube is detachably connected with the amplification tube;
sampling tube grooves are symmetrically formed in the bottom of the sampling tube by taking the capillary tube as an axis;
one end of the capillary tube is embedded at the bottom of the sampling tube, and the end part of the capillary tube is higher than the bottom plane of the sampling tube; the other end of the capillary tube extends to the amplification tube through the bottom of the sampling tube;
the wax film is provided at the end of a capillary extending to the end of the amplification tube.
2. The thermal expansion and cold contraction type integrated reaction tube according to claim 1, wherein a sampling tube buckling protrusion is arranged on a bottom cylindrical surface of the sampling tube, an amplification tube buckling groove is arranged on an upper inner layer of the amplification tube, and the sampling tube buckling protrusion and the amplification tube buckling groove form a trapezoidal buckling structure;
the sampling tube and the inclined plane of the amplification tube are assembled and sealed in an interference manner.
3. The thermal expansion and cold contraction type integrated reaction tube according to claim 1, wherein a handle is provided on the top of the sampling cap, convex-concave anti-slip patterns are provided on the outer surface of the sampling cap, threads are provided on the inner surface of the sampling cap, and a sealing ring is provided on the top of the inner surface of the sampling cap.
4. The thermally expanding and cooling shrinkage type integral reaction tube as set forth in claim 1, wherein the melting point of the wax film is 40 ℃ to 95 ℃.
5. The thermal expansion and contraction type integrated reaction tube according to claim 1, wherein the bottom of the amplification tube is designed to be transparent, and the light transmittance is 80% or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110490207.1A CN113249196A (en) | 2021-05-06 | 2021-05-06 | Thermal expansion and cold contraction type integrated reaction tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110490207.1A CN113249196A (en) | 2021-05-06 | 2021-05-06 | Thermal expansion and cold contraction type integrated reaction tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113249196A true CN113249196A (en) | 2021-08-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110490207.1A Pending CN113249196A (en) | 2021-05-06 | 2021-05-06 | Thermal expansion and cold contraction type integrated reaction tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113249196A (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2070107A1 (en) * | 1991-06-07 | 1992-12-08 | John L. Haynes | Capillary tube assembly including a vented cap |
| CN201007697Y (en) * | 2006-12-31 | 2008-01-16 | 中国科学院工程热物理研究所 | High temperature sampling probe for researching combustion |
| CN105039149A (en) * | 2015-07-20 | 2015-11-11 | 宁波大学 | Closed experiment system device for quickly identifying nucleic acid amplification products and application of closed experiment system device |
| WO2016180333A1 (en) * | 2015-05-12 | 2016-11-17 | 厦门大学 | Nucleic acid amplification reaction tube capable of controlling liquid circulation path |
| CN106222068A (en) * | 2016-08-22 | 2016-12-14 | 上海交通大学 | Miniature PCR system of capillary glass tube and preparation method thereof |
| CN109529963A (en) * | 2017-09-21 | 2019-03-29 | 纬创资通股份有限公司 | Automatic liquid transfer equipment and liquid transfer module thereof |
| CN208694940U (en) * | 2018-06-30 | 2019-04-05 | 朱三宝 | A kind of autoclave |
| CN211177251U (en) * | 2019-12-18 | 2020-08-04 | 广州森科节能科技有限公司 | Cooling and dust-proof device for electric room |
| CN112137791A (en) * | 2019-06-29 | 2020-12-29 | 新沂市慧聚医药产业投资管理有限公司 | Medical infusion warming device |
| CN212864768U (en) * | 2020-07-22 | 2021-04-02 | 陆千千 | Be used for clinical little biological detection sampling device of medical science |
| CN112594921A (en) * | 2020-12-28 | 2021-04-02 | 陈晓东 | Enzyme reaction device with self-heating water supply |
-
2021
- 2021-05-06 CN CN202110490207.1A patent/CN113249196A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2070107A1 (en) * | 1991-06-07 | 1992-12-08 | John L. Haynes | Capillary tube assembly including a vented cap |
| CN201007697Y (en) * | 2006-12-31 | 2008-01-16 | 中国科学院工程热物理研究所 | High temperature sampling probe for researching combustion |
| WO2016180333A1 (en) * | 2015-05-12 | 2016-11-17 | 厦门大学 | Nucleic acid amplification reaction tube capable of controlling liquid circulation path |
| CN105039149A (en) * | 2015-07-20 | 2015-11-11 | 宁波大学 | Closed experiment system device for quickly identifying nucleic acid amplification products and application of closed experiment system device |
| CN106222068A (en) * | 2016-08-22 | 2016-12-14 | 上海交通大学 | Miniature PCR system of capillary glass tube and preparation method thereof |
| CN109529963A (en) * | 2017-09-21 | 2019-03-29 | 纬创资通股份有限公司 | Automatic liquid transfer equipment and liquid transfer module thereof |
| CN208694940U (en) * | 2018-06-30 | 2019-04-05 | 朱三宝 | A kind of autoclave |
| CN112137791A (en) * | 2019-06-29 | 2020-12-29 | 新沂市慧聚医药产业投资管理有限公司 | Medical infusion warming device |
| CN211177251U (en) * | 2019-12-18 | 2020-08-04 | 广州森科节能科技有限公司 | Cooling and dust-proof device for electric room |
| CN212864768U (en) * | 2020-07-22 | 2021-04-02 | 陆千千 | Be used for clinical little biological detection sampling device of medical science |
| CN112594921A (en) * | 2020-12-28 | 2021-04-02 | 陈晓东 | Enzyme reaction device with self-heating water supply |
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| PB01 | Publication | ||
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Country or region after: China Address after: Room 409-53, 4th floor, building 1, No. 38 Yongda Road, Daxing biomedical industry base, Zhongguancun Science Park, Daxing District, Beijing 102600 Applicant after: Beijing Aeonview Co.,Ltd. Address before: Room 409-53, 4th floor, building 1, No. 38 Yongda Road, Daxing biomedical industry base, Zhongguancun Science Park, Daxing District, Beijing 102600 Applicant before: Beijing Yian Hejing Biotechnology Co.,Ltd. Country or region before: China |