CN113214212B - Preparation method of lycium ruthenicum murr green anthocyanin - Google Patents
Preparation method of lycium ruthenicum murr green anthocyanin Download PDFInfo
- Publication number
- CN113214212B CN113214212B CN202110449075.8A CN202110449075A CN113214212B CN 113214212 B CN113214212 B CN 113214212B CN 202110449075 A CN202110449075 A CN 202110449075A CN 113214212 B CN113214212 B CN 113214212B
- Authority
- CN
- China
- Prior art keywords
- inorganic salt
- anthocyanin
- organic solvent
- lycium ruthenicum
- aqueous solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229930002877 anthocyanin Natural products 0.000 title claims abstract description 50
- 235000010208 anthocyanin Nutrition 0.000 title claims abstract description 50
- 239000004410 anthocyanin Substances 0.000 title claims abstract description 50
- 150000004636 anthocyanins Chemical class 0.000 title claims abstract description 50
- 241000169546 Lycium ruthenicum Species 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title description 5
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 23
- 239000003960 organic solvent Substances 0.000 claims abstract description 15
- 239000000040 green colorant Substances 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 19
- 239000007864 aqueous solution Substances 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 9
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical group [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 235000019441 ethanol Nutrition 0.000 claims description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 3
- 238000002137 ultrasound extraction Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 239000003086 colorant Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000000691 measurement method Methods 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000004438 eyesight Effects 0.000 description 2
- -1 flavonoid compounds Chemical class 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000002932 luster Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- JPFCOVZKLAXXOE-XBNSMERZSA-N (3r)-2-(3,5-dihydroxy-4-methoxyphenyl)-8-[(2r,3r,4r)-3,5,7-trihydroxy-2-(4-hydroxyphenyl)-3,4-dihydro-2h-chromen-4-yl]-3,4-dihydro-2h-chromene-3,5,7-triol Chemical compound C1=C(O)C(OC)=C(O)C=C1C1[C@H](O)CC(C(O)=CC(O)=C2[C@H]3C4=C(O)C=C(O)C=C4O[C@@H]([C@@H]3O)C=3C=CC(O)=CC=3)=C2O1 JPFCOVZKLAXXOE-XBNSMERZSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001106041 Lycium Species 0.000 description 1
- 208000037093 Menstruation Disturbances Diseases 0.000 description 1
- 206010027339 Menstruation irregular Diseases 0.000 description 1
- 240000002624 Mespilus germanica Species 0.000 description 1
- 235000017784 Mespilus germanica Nutrition 0.000 description 1
- 235000000560 Mimusops elengi Nutrition 0.000 description 1
- 229920001991 Proanthocyanidin Polymers 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 206010057040 Temperature intolerance Diseases 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 235000007837 Vangueria infausta Nutrition 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000000038 blue colorant Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 229940124600 folk medicine Drugs 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 230000008543 heat sensitivity Effects 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000008265 rhamnosides Chemical class 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a method for extracting and preparing green anthocyanin from lycium ruthenicum, which mainly adopts the modes of inorganic salt, organic solvent, and the like. The anthocyanin obtained by the method has similar color characteristics with bright green (CAS: 18015-76-4), and can be used as natural substitute for chemical synthetic green colorant.
Description
Technical Field
The invention relates to the technical field of plant extraction, in particular to a preparation method of lycium ruthenicum murr green anthocyanin.
Background
Lycium ruthenicum (Lycium ruthenicum Murr.) belonging to the genus Lycium of Solanaceae is mainly distributed in China and in Qinghai, ningxia, xinjiang, gansu and Shaanxi etc., and is a perennial shrub wild plant specific to arid regions of China. According to the records of four medical classics, *** medicine uses lycium ruthenicum for treating heart heat disease, heart disease, irregular menstruation, menstruation stopping and other diseases; the folk medicines are used as tonic, eyesight improving and blood pressure lowering medicines. Researches show that the lycium ruthenicum contains a plurality of active ingredients such as polysaccharide, flavone, proanthocyanidin, anthocyanin, phenolic acid and the like, and has the effects of resisting fatigue, reducing blood sugar, resisting oxidation, inhibiting tumors, nourishing, protecting health and the like.
Anthocyanin (Anthocyanins) is a water-soluble natural pigment widely existing in plants in nature, and belongs to flavonoid compounds. Anthocyanin exists mainly in the forms of glucoside, rhamnoside and the like and is contained in fruits, epidermis and flowers of plants. The anthocyanin content in the lycium ruthenicum is extremely high, wherein more than 90% of anthocyanin is petuniin derivatives, and the lycium ruthenicum has the functions of resisting oxidation, protecting eyesight, inhibiting tumors and the like, and has important biological activity and extraction value. However, the lycium ruthenicum anthocyanin has the characteristics of easy oxidization, heat sensitivity, extremely poor stability and the like, and the purity of the anthocyanin can be directly influenced by a large amount of sugar chains connected through glycosidic bonds, so that the extraction, separation and purification are difficult, and the effective extraction rate of the lycium ruthenicum anthocyanin in the prior art is low.
At present, it is reported at home and abroad that lycium ruthenicum anthocyanin is prepared by taking lycium ruthenicum fruits as raw materials and adopting a solvent extraction method and assisting modern technologies such as ultrasound, microwave and enzyme, and the obtained lycium ruthenicum anthocyanin is generally magenta, and the aqueous solution of the lycium ruthenicum anthocyanin is generally magenta, and only at a specific pH value, the lycium ruthenicum anthocyanin has similar color characteristics as brilliant blue (CAS: 3844-45-9). The natural blue anthocyanin-containing colorant described in patent publication No. CN105339437A, and the anthocyanin described in patent publication No. CN109574979A is a blue colorant. However, no report on the natural coloring agent of the green anthocyanin is found.
From the above, it is found that natural green colorants containing anthocyanin have not been reported. Therefore, the lycium ruthenicum anthocyanin which is simple and efficient to prepare and green in anthocyanin has important significance and good market prospect.
Disclosure of Invention
In order to solve the problems, the invention relates to a simple and efficient method for providing lycium ruthenicum anthocyanin with green anthocyanin.
Specifically, the invention provides a method for extracting and preparing green anthocyanin from lycium ruthenicum, which comprises the following steps:
(1) Adding the lycium ruthenicum ultrafine powder into an inorganic salt A aqueous solution and a hydrophilic low-molecular organic solvent, extracting, standing and phase-separating;
(2) Removing the upper phase solvent, adding absolute ethyl alcohol to separate out inorganic salt, filtering, removing the absolute ethyl alcohol from the liquid part to obtain solid matters, redissolving the solid matters in the inorganic salt B aqueous solution, standing, and removing the solvent under reduced pressure to obtain green anthocyanin;
the inorganic salt A is selected from one or more than two of sodium chloride, ammonium sulfate, monopotassium phosphate and dipotassium phosphate; the hydrophilic low molecular organic solvent is one or more of methanol, ethanol, acetone, isopropanol and n-butanol; the inorganic salt B is selected from magnesium chloride: calcium chloride: the aluminum chloride was 0.3M:0.8M:0.2M.
Further, the inorganic salt a is selected from ammonium sulfate.
Further, the hydrophilic low molecular organic solvent is selected from ethanol.
Wherein, in the step (2), the solid matters are redissolved in the inorganic salt B aqueous solution with the concentration of 0.1-3mg/mL and 0-2 mol/L.
Wherein the hydrophilic low molecular organic solvent accounts for 20-40% of the total mass of the inorganic salt A aqueous solution and the hydrophilic low molecular organic solvent.
Wherein the inorganic salt A accounts for 15-25% of the total mass of the inorganic salt A aqueous solution and the hydrophilic low molecular organic solvent.
Wherein, the total mass ratio of the lycium ruthenicum ultrafine powder to the inorganic salt A aqueous solution to the hydrophilic low-molecular organic solvent is 1:5-1:20.
wherein, step (1) adopts ultrasonic extraction.
The invention also provides the green anthocyanin prepared by the method.
The invention also provides a green colorant, which comprises the green anthocyanin.
The black fruit medlar green anthocyanin provided by the invention: the aqueous solution has absorption peaks at 500-550 nm and 700-780 nm and a hue angle h ab The value is 120-150; the powder was a greenish black powder having a metallic luster and a highest reflection peak at 780 nm.
The method can effectively obtain the lycium ruthenicum murr green anthocyanin, and has the following advantages compared with the existing green colorant:
(1) The anthocyanin has similar color characteristics with bright green (CAS: 18015-76-4), and can be used as natural substitute for chemical synthetic green colorant;
(2) Simple process steps, refined working procedures and high preparation efficiency.
Drawings
FIG. 1 is a spectrophotometric spectrum of the product;
FIG. 2 CIE1931 observer graph;
fig. 3 is a CIE1931 observer graph after treatment with various inorganic salts.
Detailed Description
In order that the invention may be more readily understood, it is further described below in connection with specific examples which are intended to be in no way limiting, but are intended to be in any way limiting, and any modifications or alterations which would be readily apparent to a person of ordinary skill in the art without departing from the technical solutions of the present invention will fall within the scope of the claims of the present invention.
Example 1A green anthocyanin of Lycium ruthenicum Murr and its preparation method
(1) Pulverizing dried Lycium ruthenicum Murr with superfine pulverizer, and sieving with 100 mesh sieve to obtain superfine powder;
(2) Weighing 1.7g of ammonium sulfate, adding 5.9g of water to prepare an aqueous solution, then adding 2.4g of absolute ethyl alcohol to prepare an alcohol-ammonium sulfate double-aqueous-phase system, adding 1.0g of lycium ruthenicum ultrafine powder into the double-aqueous-phase system, carrying out ultrasonic assisted extraction for 30min, and standing for 1h at room temperature to layer;
(3) After rotationally evaporating the solvent at the upper phase of the step (2) at 50 ℃, dissolving and filtering the solvent with absolute ethyl alcohol to recover inorganic salt, rotationally evaporating the solvent at 50 ℃ again to recover absolute ethyl alcohol to obtain 50 mg of the product, measuring the anthocyanin content to be 14%, and re-dissolving the anthocyanin in a mixed ion solution with the concentration of 0.2mg/mL, wherein each ion magnesium chloride in the ion solution: calcium chloride: the molar concentration ratio of aluminum chloride is 0.3M to 0.8M to 0.2M, the mixture is kept stand and balanced for 20min to wait for the reaction to be complete, and the solvent is evaporated at 60 ℃ by a rotary evaporator to obtain the green anthocyanin.
Analytical evaluation
The sample is measured at 380-780 n by reference to the specification of pages 2-5 of the color space method CIE1976 (Lx a x b) for measuring the color of SN/T4675.25-2016 outlet wineThe absorbance in the m wavelength range is integrated by a relative color stimulus function and a color matching function, and then compared with a standard tristimulus value, the hue angle h of the sample is calculated ab A value; the method comprises the steps of referring to the specification of pages 2-4 of GB/T3979-2008 object color measurement method, measuring the reflection spectrum of a sample, and calculating the hue angle hab value of the sample; the anthocyanin content of the samples was determined with reference to the provisions of AOAC Official Method 2005.02 Total Monomeric Anthocyanin Pigment Content of Fruit Juices, beverages, natural Colorants and Wines pH Differential Method First Action 2005 (J. AOAC Int. 88, 1269 (2005)).
Through testing, the 0.2mg/mL aqueous solution of lycium ruthenicum anthocyanin A prepared by the method has absorption peaks at 500-550 nm and 700-780 nm and h as shown in figure 1 ab Values 130.24, as shown in fig. 2, are the positions of the aqueous solution at CIE1931 observer coordinates; the pH value is 7.0; lycium ruthenicum anthocyanin A is dark green powder with metallic luster, has an absorption peak at 520nm and has a highest reflection peak at 780 nm; the anthocyanin content of lycium ruthenicum anthocyanin A is 12.5%.
Test example 1
Using the procedure in example 1, a specific ratio of magnesium chloride was explored: calcium chloride: relationship between aluminum chloride ratio and product color;
the color measurement method refers to GB/T3979-2008 object color measurement method, and the detection is carried out at the anthocyanin concentration of 0.2 mg/mL;
group 1: lycium ruthenicum anthocyanin stock solution
Group 2:0.2:0.8:0.1
Group 3:0.3:0.8:0.2
Group 4:0.2:0.8:0.2
The results are shown in FIG. 3
As can be seen from fig. 3, by adjusting the ratio between the individual chlorinated metals to achieve different colors, the reason is that the metals and the anthocyanins combine to produce complexes, and different complexes and complexing ratios produce different colors, so that to achieve green we can choose group 3 as the optimal color-changing formulation.
Claims (9)
1. The method for extracting and preparing the green anthocyanin from the lycium ruthenicum is characterized by comprising the following steps of: it comprises the following contents:
(1) Adding the lycium ruthenicum ultrafine powder into an inorganic salt A aqueous solution and a hydrophilic low-molecular organic solvent, extracting, standing and phase-separating;
(2) Removing the upper phase solvent, adding absolute ethyl alcohol to separate out inorganic salt, filtering, removing the absolute ethyl alcohol from the liquid part to obtain solid matters, redissolving the solid matters in the inorganic salt B aqueous solution, standing, and removing the solvent under reduced pressure to obtain green anthocyanin;
the inorganic salt A is ammonium sulfate; the hydrophilic low molecular organic solvent is one or more of methanol, ethanol, acetone, isopropanol and n-butanol; the inorganic salt B is selected from magnesium chloride: calcium chloride: the aluminum chloride was 0.3M:0.8M:0.2M.
2. The method according to claim 1, characterized in that: the hydrophilic low molecular organic solvent is selected from ethanol.
3. The method according to claim 1, characterized in that: in the step (2), the solid is redissolved in the inorganic salt B aqueous solution at the concentration of 0.1-3 mg/mL.
4. The method according to claim 1, characterized in that: the hydrophilic low molecular organic solvent accounts for 20-40% of the total mass of the inorganic salt A aqueous solution and the hydrophilic low molecular organic solvent.
5. The method according to claim 1, characterized in that: the inorganic salt A accounts for 15-25% of the total mass of the inorganic salt A aqueous solution and the hydrophilic low-molecular organic solvent.
6. The method according to claim 1, characterized in that: the total mass ratio of the lycium ruthenicum ultrafine powder to the inorganic salt A aqueous solution to the hydrophilic low-molecular organic solvent is 1:5-1:20.
7. the method according to claim 1, characterized in that: and (3) ultrasonic extraction is adopted in the step (1).
8. A green anthocyanin prepared by the method of any one of claims 1 to 7.
9. A green colorant characterized by: comprising the green anthocyanin of claim 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110449075.8A CN113214212B (en) | 2021-04-25 | 2021-04-25 | Preparation method of lycium ruthenicum murr green anthocyanin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110449075.8A CN113214212B (en) | 2021-04-25 | 2021-04-25 | Preparation method of lycium ruthenicum murr green anthocyanin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113214212A CN113214212A (en) | 2021-08-06 |
| CN113214212B true CN113214212B (en) | 2023-09-29 |
Family
ID=77088891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110449075.8A Active CN113214212B (en) | 2021-04-25 | 2021-04-25 | Preparation method of lycium ruthenicum murr green anthocyanin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113214212B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101531652A (en) * | 2009-04-17 | 2009-09-16 | 大连理工大学 | Novel method for extracting anthocyanin in bilberry by double water phases |
| CN109574979A (en) * | 2018-12-28 | 2019-04-05 | 中国科学院西北高原生物研究所湖州高原生物资源产业化创新中心 | A kind of black fruit fructus lycii blue anthocyanidin and preparation method thereof |
| CN109575633A (en) * | 2018-12-28 | 2019-04-05 | 中国科学院西北高原生物研究所湖州高原生物资源产业化创新中心 | A kind of black fruit fructus lycii blue extract and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2641830C2 (en) * | 2013-03-15 | 2018-01-22 | Марс, Инкорпорейтед | Natural blue anthocyanine-containing dyes |
-
2021
- 2021-04-25 CN CN202110449075.8A patent/CN113214212B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101531652A (en) * | 2009-04-17 | 2009-09-16 | 大连理工大学 | Novel method for extracting anthocyanin in bilberry by double water phases |
| CN109574979A (en) * | 2018-12-28 | 2019-04-05 | 中国科学院西北高原生物研究所湖州高原生物资源产业化创新中心 | A kind of black fruit fructus lycii blue anthocyanidin and preparation method thereof |
| CN109575633A (en) * | 2018-12-28 | 2019-04-05 | 中国科学院西北高原生物研究所湖州高原生物资源产业化创新中心 | A kind of black fruit fructus lycii blue extract and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 双水相萃取分离葡萄籽中原花青素;张喜峰;程广;何倩;张芬琴;罗光宏;;食品与机械(03);168-173 * |
| 盐诱导乙醇水体系萃取分离葡萄皮中原花青素;张喜峰;何倩;张斌;王明卉;罗光宏;;中国酿造(05);139-143 * |
| 金属离子对黑果枸杞中花青素颜色稳定性的影响;孟庆昕;高逸雯;姚思敏蔷;吕远平;;湖北农业科学(22);113-117 * |
| 青藏高原黑果枸杞花青素稳定性评价;陈莎莎;索有瑞;白波;王洪伦;胡娜;;天然产物研究与开发(02);322-328 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113214212A (en) | 2021-08-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Tural et al. | Physico-chemical and antioxidant properties of cornelian cherry fruits (Cornus mas L.) grown in Turkey | |
| CN109574979B (en) | Lycium ruthenicum Murr blue anthocyanin and preparation method thereof | |
| CN101735644B (en) | Method for extracting ipomoes batatas L. anthocyanidins | |
| Jadhav et al. | Free radical scavenging and antioxidant activity of Punica granatum Linn | |
| CN107625672A (en) | A kind of method and application for extracting plurality of active ingredients simultaneously from green gram spermoderm | |
| Pandimeena et al. | Evaluation of phytochemicals and in vitro antiinflammatory, anti-diabetic activity of the white oyster mushroom, Pleurotus florida | |
| CN104983915B (en) | A kind of preparation method of black fruit fructus lycii natural composite antioxidant | |
| CN104544456B (en) | Sea-buckthorn type solid dietary fiber beverage and preparation method thereof | |
| CN101643589A (en) | Extraction method of natural sunflower seed hull melanin | |
| Tahirović et al. | Phenolic content and antioxidant activity of Crataegus monogyna L. fruit extracts | |
| CN111903886A (en) | Dendrobium and rosa roxburghii tratt compound solid beverage and brewing method thereof | |
| CN113214212B (en) | Preparation method of lycium ruthenicum murr green anthocyanin | |
| CN106018296A (en) | Method for measuring content of total flavonoids in blueberries | |
| CN108531356A (en) | Jet rice vinegar and the method for extracting jet anthocyanin | |
| Dimcheva et al. | ANTIOXIDANT ACTIVITY AND POLYPHENOLIC CONTENT OF THE BULGARIAN WILD HERB Cistus incanus L. STORED UNDER DIFFERENT CONDITIONS. | |
| Hussain et al. | Comparative in vitro analysis of anti-diabetic activity of Indo-Pak black cardamom (Amomum subulatum Roxb.) and Chinese black cardamom (Amomum tsao-ko Crevost et Lemaire) | |
| CN113337141B (en) | Preparation method of lycium ruthenicum murr yellow anthocyanin | |
| Kabra et al. | Total phenolics & flavonoid content of the leaves of Carica papaya & Syzygium cumini | |
| CN106512018B (en) | A kind of refined honey method suitable for honeyed bolus | |
| Prashanth et al. | In vitro antioxidant studies of Momordica cymbalaria. | |
| KR101971362B1 (en) | Food composition containing anthocyanin extracted from amaranth | |
| Hewageegana et al. | Comparison on phytochemical and physicochemical parameters of Garcinia cambogia (Gaertn.) Desr. and Garcinia zeylanica Linn fruit rinds | |
| KR101716342B1 (en) | Extralation process for anthocyanidin from the sorghum | |
| CN108936216A (en) | A kind of desugar technique of sea buckthorn juice | |
| Anand et al. | Quantitative evaluation of plant metabolites and dye extraction from leaves of Ampelocissus latifolia (Roxb.) planch |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 313098 9 / F, South Taihu science and technology innovation center, 1366 Hongfeng Road, Huzhou City, Zhejiang Province Patentee after: Huzhou Zhongke plateau Biological Innovation Center Country or region after: China Address before: 9 / F, South Taihu science and technology innovation center, 1366 Hongfeng Road, Huzhou City, Zhejiang Province, 313000 Patentee before: HUZHOU PLATEAU BIOLOGICAL RESOURCES INDUSTRY INNOVATION CENTER OF NORTHWEST INSTITUTE OF PLATEAU BIOLOGY, CHINESE ACADEMY OF SCIENCES Country or region before: China |
|
| CP03 | Change of name, title or address |