CN1132032A

CN1132032A - Pig feed fermentation agent and its permentation tech.

Info

Publication number: CN1132032A
Application number: CN95102615A
Authority: CN
Inventors: 王厚德
Original assignee: Individual
Current assignee: Individual
Priority date: 1995-03-24
Filing date: 1995-03-24
Publication date: 1996-10-02

Abstract

A fermenting agent for pig feed is prepared through respectively culturing each of EM-1 bacteria, common beneficial bacteria and yeasts and proportionally mixing their solid resultants, and is used by mixing it with part of three kinds of feed, fermenting and mixing fermented feed with unfermented rest and water. It features high digestion and absorbing rate of feed, speeding up growth of pig, and reducing infectious disease of digestive tract.

Description

A kind of novel pig feed starter and fermentation technique thereof

(1) a kind of novel pig feed starter and fermentation technique belong to using microbe and feed technology field.

(2) prior art and deficiency thereof

The feed fermentation technology was once gone in China the beginning of the seventies once.Middle Other-of fermented feed at that time is mainly based on straw fermenting, grass meal.But because the digestibility of its leavened prod is very unobvious, so do not re-use this technology at present substantially.

To studies show that of China's feeds utilized present situation in rural culture: contain a large amount of Mierocrystalline celluloses and carbohydrate in most feeds, and livestock is very low to the digestibility of this class material.In recent years, the existing both at home and abroad technology that adopts the higher feed of cellulose-decomposing bacteria (comprising actinomycetes, some monoid of mould) fermentation crude fiber content.This technology has certain effect to economic benefit that improves aquaculture and the utilization ratio that improves the high feed of crude fiber content, but effect is not remarkable, therefore, fails to promote the use of widely in the whole nation.As for being fermented, carbohydrate then do not see any report.

(3) goal of the invention

The present invention is directed to some deficiency in the existing domestic and international feed fermentation technology, mainly reach following purpose:

1, the digestibility of the roughage that the digestibility, particularly carbohydrate of raising pig feed and crude fiber content are higher.

2, increase the probiotic bacterium content in the feed.Help keeping the microecological balance of swine alimentary canal, thereby improve the digestibility and the immunity of organisms of feed, control swine alimentary canal transmissible disease.

3, improve the palatability of pig feed.

(4) core content of the present invention

1, the fermented bacterium combination that a kind of novel pig feed starter is made up of following three fungus strains:

(1) employing is from " EM-1 " fungus strain (wherein containing beneficial microorganisms such as photosynthetic bacterium, lactobacillus, yeast) that is used for food or feed of external introduction.

(2) domestic probiotic bacterium commonly used is, comprising: bacillus cereus (aerobic bacteria) and bifidus bacillus (anerobe) totally two strains.

(3) yeast: Endomycopsis Fibnligera.

The ratio of above-mentioned three groups of fermented bacteriums is: (1): (2): (3)=8: 1.5: 0.5, this fungus strain portfolio ratio obtains by Orthogonal experiment results.

2, be the pig feed fermentation technique of bacterial classification with above-mentioned starter:

(1) partly ferment at three kinds of dissimilar pig feeds: to the valency mixed feed, the fermentation wherein 70%; To the mixed fodder of concentrated fodder and energy feed, a fermentation energy feed wherein; To the mixed fodder of concentrated feed and roughage, energy feed in the ferment essence feed and roughage.

(2) key problem in technology of the present invention is the water content of fermented product.For reaching best effect, when adopting this ferment-fermented, in fermentation materials, only add the water about 30%.

(5) the concrete implementation step of the present invention

1, this starter is made

(1) import " EM-1 " fungus strain is cultivated:

1. cultivate flow process:

Commercially available second class inoculum (liquid) → three-class strain (fluid enlargement culture) → level Four bacterial classification (solid culture) → cryodrying (below 80 ℃) → pulverizing.

2. three-class strain is made:

With potato (10%), wheat bran (2-5%), water (85-88%) are substratum (liquid state), liquor; The liquor after-filtration; The filtrate autoclaving; Inoculation; Leave standstill cultivation.

3. the level Four bacterial classification is made

With bean cake powder (60%); Fish meal (10%); (corn 20%); (wheat bran 10%) is substratum; Raw material pulverizing; Be mixed in proportion; Profit water; Autoclaving; Connect three-class strain; Under aseptic condition, cultivate; Cryodrying; Pulverize.

(2) domestic probiotic bacterium is to cultivate:

Aerobic bacillus cereus and anaerobic bifidobacterium are cultivated respectively, and tunning solidifies the back by 1: 1 mixed promptly.

1. cultivate flow process:

Inclined-plane → second class inoculum (liquid culture) → three-class strain (solid culture) → cryodrying (below 80 ℃) → pulverizing.

2. bacterial classifications at different levels are made:

A, inclined-plane (solid):

With peptone (0.5%), agar (2%), extractum carnis (0.3%), yeast extract paste (0.3%), Nacl (0.5%), KH ₂PO ₄(0.3%), MgSO ₄(0.1%) be substratum, PH is 7.2; The raw material autoclaving; Inoculation; Cultivate.

B, second class inoculum (liquid):

The same inclined-plane of culture medium prescription does not just add agar, and PH still is 7.2; Bottling; Autoclaving; Connect slant strains; Leave standstill cultivation.

C, three-class strain (solid):

With dregs of beans (20-25%), corn (30-40%), wheat bran (10-20%), rice (5-10%), mung bean protein powder (10-20%) is a substratum; Raw material is mixed in proportion after crushed; Profit water; Autoclaving; Connect second class inoculum; At the aseptic condition bottom fermentation; Cryodrying; Pulverize.

The cultivation of (3) yeast--Endomycopsis Fibnligera

1. cultivate flow process:

Inclined-plane → second class inoculum (solid culture) → three-class strain (solid enlarged culturing) → cryodrying → pulverizing.

2. bacterial classifications at different levels are made:

A, the inclined-plane:

With wort, agar is substratum, inoculation culture behind high-temperature sterilization.

B, second class inoculum (solid);

With corn (40%), dregs of beans (20%), wheat bran (10%), Zein powder (20%), cottonseed cake (10%) is a substratum; Raw material is mixed in proportion after crushed; Profit water; Sterilization; Inoculation; Be sub-packed in the enamel tray of tool lid.Aseptic condition is cultivated down; Low temperature (below 80 ℃) drying; Pulverize.

C, three-class strain (solid):

Prescription and all same second class inoculum of operating procedure, different is the bigger fermentation dish of fermentation instrument usable floor area.

(4) bacterial classification mixes:

In " EM-1 " fungus strain: domestic probiotic bacterium system: the ratio of yeast=8: 1.5: 0.5 mixes bacterial classification, promptly becomes a kind of novel pig feed starter.

The feed that will ferment, the ratio in about 30% adds sterilized water, and inoculation stirs simultaneously, and pack into cylinder or cement pit or Polywoven Bag all can.Sealing and fermenting 24-48 hour (time decides on temperature), when treating that fermentation materials accumulated temperature reaches 760 ℃, stuck fermentation.The nonfermented part is sneaked into fermentation part in proportion, be used for feeding and raise, in two days, fed.

At three kinds of different pig feeds, we operate as follows respectively:

1. full price is cooperated pig feed:

Only fermentation wherein 70%.Add 30% water; Inoculum size 0.3%; Fermented 24-48 hour.

2. to the mixed fodder that adds energy feed of concentrated fodder:

Fermentation energy feed wherein only.Add water 30%; Inoculum size 0.3%; Fermented 24-48 hour.

3. the mixed fodder that concentrated feed is added roughage:

Only the energy feed in roughage and the concentrated feed (as rice, corn) is fermented.To protein fodder composition (fish meal, dregs of beans etc.) and all nonfermented such as trace element and other additive, mineral substance.Add water 30%; Inoculum size 0.3%; Fermented 24-48 hour.

(6) advantage and the key technical indexes:

1, use this ferment-fermented pig's feed to have the following advantages:

1. the feed digestibility can improve 10-20%;

2. under the constant situation of feedstuff-meat ratio, the rate of putting on flesh of pig improves 10-20%;

3. good palatability;

4. the infectious disease of the digestive tract of pig reduces about 90%.

5. user's economic benefit improves about 15%;

Amount of water is less during 6. owing to fermentation, and feed can be not agglomerating, is applicable to mechanized feeding.

2, the key technical indexes of this ferment-fermented pig's feed of use:

1. outward appearance and Oranoleptic indicator

(1) outward appearance: color is darker slightly than former material color.

(2) smell: have the fermented product special odor, slightly the fume flavor.

(3) flavour: slightly sweet tart flavour.

2. microbiological indicator:

Total plate count 〉=4,000,000,000/gram dry material,

Yeast sum 〉=2.0 hundred million/gram dry material.

(dry material means the fermentation part of feed).

Reference:

1, [English] R.F. Stanberry, A, the Hui Teke work: " fermentology principle " period-luminosity Shao, Chen Daijie translates, Chinese Medicine science and technology press, 1992.

2, Pan Linyang, Guan Xinfu chief editor: " feed ", Hubei science and technology press, 1993.

3, LiBing Tan, Zhao Shuguang, Guo Chuanjia chief editor: " Swine Production technical manual ", agriculture press, 1990.

Claims

1, a kind of novel pig feed starter, it is characterized in that: with " EM-1 " fungus strain of import, probiotic bacterium system domestic commonly used and some yeast of being made up of bacillus cereus (aerobic) and bifidus bacillus (anaerobism) carry out all grade of cultivation respectively, and the mixed that its cured product is determined by orthogonal experiment forms the novel fermentation agent.

2, according to the described novel pig feed starter of claim 1, it is characterized in that: said " EM-1 " fungus strain to import carries out all grade of cultivation and is meant commercially available second class inoculum (liquid), cultivate through three grades (liquid), level Four (solid) is cultivated, and low temperature (below 80 ℃) drying and pulverizing form cured product.

3, according to the described novel pig feed starter of claim 1, it is characterized in that: saidly domestic probiotic bacterium system is carried out all grade of cultivation be meant aerobic bacillus cereus and anaerobic bifidobacterium are carried out slant culture separately respectively, secondary (liquid) is cultivated, three grades (solid) cultivated, and low temperature (below 80 ℃) drying and pulverizing form cured product.

4, according to the described novel pig feed starter of claim 1, it is characterized in that: saidly yeast is carried out all grade of cultivation be meant the Endomycopsis Fibnligera bacterial strain through slant culture, secondary (solid) is cultivated, three grades of expansions (solid) are cultivated, and low temperature (below 80 ℃) drying and pulverizing form cured product.

5, according to the described novel pig feed starter of claim 1, it is characterized in that: said three groups of bacterial classifications are meant that by the definite blending ratio of orthogonal experiment the optimum mixture ratio example of three kinds of products is 8: 1.5: 0.5 through all grade of cured product of cultivating gained.

6, according to the described novel pig feed starter of claim 1, use the technology of its fermentation pig feed, it is characterized in that: with this starter dissimilar pig feeds are carried out the part fermentation and be meant that this is bacterial classification (by 0.3% inoculum size) with the starter, and the feed of fermentation part added 30% water, fermented 24-48 hour, wherein:

1. full price is cooperated pig feed, only fermentation wherein 70%;

2. the mixed fodder that concentrated fodder is added energy feed, only fermentation energy feed wherein;

3. the mixed fodder that concentrated feed is added roughage, energy feed and roughage in the ferment essence feed.