CN113197313B - Probiotic composition with effects of improving insulin resistance and fasting blood glucose and preparation method thereof - Google Patents
Probiotic composition with effects of improving insulin resistance and fasting blood glucose and preparation method thereof Download PDFInfo
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- CN113197313B CN113197313B CN202010911082.0A CN202010911082A CN113197313B CN 113197313 B CN113197313 B CN 113197313B CN 202010911082 A CN202010911082 A CN 202010911082A CN 113197313 B CN113197313 B CN 113197313B
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- bifidobacterium
- probiotic composition
- insulin resistance
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention relates to the field of functional foods, in particular to a probiotic composition with the functions of improving insulin resistance and fasting blood glucose and a preparation method thereof, wherein the probiotic composition with the functions of improving insulin resistance and fasting blood glucose comprises the following components: bifidobacterium lactis (Bifidobacterium lactis) MN-Gup, lactobacillus acidophilus (Lactobacillus acidophilus) NCFM, bifidobacterium lactis (Bifidobacterium lactis) B420, bifidobacterium adolescentis (Bifidobacterium adolescentis) BA02 and lactobacillus reuteri (Lactobacillus ruteri) SD5865; the probiotics in the probiotic composition cooperatively play roles of reducing insulin resistance index and reducing fasting blood glucose.
Description
Technical Field
The invention relates to the field of functional foods, in particular to a probiotic composition with the functions of improving insulin resistance and fasting blood glucose and a preparation method thereof.
Background
Diabetes mellitus is a group of metabolic diseases characterized by an elevated level of chronic blood glucose (abbreviated as blood glucose), the prevalence of which increases rapidly with the increasing level of living of people, and changes in lifestyle have become the third most non-infectious disease after cardiovascular disease and tumor in developed countries, which can cause multiple system damage, cause chronic progressive changes in tissues such as eyes, kidneys, nerves, heart and blood vessels, cause functional defects and failure, and seriously threaten human health. Diabetes mellitus is classified into type 1 diabetes, type 2diabetes, gestational diabetes and other types of diabetes, depending on the cause of the disease. Type 2diabetes (t 2 dm), among others, refers to a metabolic disorder characterized by hyperglycemia caused by insulin resistance-induced destruction of islet beta cells or relative lack of insulin, and is the largest number of one subtype of diabetes.
Insulin resistance and insulin β cell secretion defects are important pathophysiological changes in the pathogenesis of T2 DM. Impaired Fasting Glucose (IFG) belongs to an intermediate state between normal glucose metabolism and diabetes, known as pre-diabetes. Any diabetic patient may go through the IFG stage during the course of the onset. Therefore, improving insulin resistance and fasting blood glucose is very important for alleviating diabetes, especially T2 DM. Therefore, the invention provides a probiotic composition with the functions of improving insulin resistance and fasting blood glucose and a preparation method thereof.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide a probiotic composition with the functions of improving insulin resistance and fasting blood glucose and a preparation method thereof.
For this purpose, the invention provides the following technical scheme:
a probiotic composition having improved insulin resistance and fasting glucose comprising: bifidobacterium lactis (Bifidobacterium lactis) MN-Gup, lactobacillus acidophilus (Lactobacillus acidophilus) NCFM, bifidobacterium lactis (Bifidobacterium lactis) B420, bifidobacterium adolescentis (Bifidobacterium adolescentis) BA02 and lactobacillus reuteri (Lactobacillus ruteri) SD5865.
Further, the weight portions are as follows:
1-50 parts of bifidobacterium lactis MN-Gup bacterial powder;
1-50 parts of lactobacillus acidophilus NCFM powder;
0.1-20 parts of bifidobacterium lactis B420 powder;
1-50 parts of bifidobacterium adolescentis BA02 bacteria powder; and
0.1-30 parts of lactobacillus reuteri SD5865 bacterial powder.
Further, the food additive also comprises food raw materials and/or food or medical acceptable auxiliary materials or additives.
Further, the food material is at least one selected from stachyose, inulin, fructo-oligosaccharide, pumpkin powder and resistant dextrin, for enhancing proliferation and efficacy effects of the probiotics.
Further, the auxiliary materials or additives comprise silicon dioxide, and are used for improving the flowability of the formula.
Further, the probiotic composition further comprises the following components in parts by weight:
stachyose, 0-100 parts;
inulin, 0-80 parts;
0-80 parts of fructo-oligosaccharide;
0-50 parts of pumpkin powder;
0-100 parts of resistant dextrin;
silica, 0-10 parts.
Further, the probiotic composition comprises the following components in parts by weight:
1-5 parts of bifidobacterium lactis MN-Gup bacterial powder;
1-5 parts of lactobacillus acidophilus NCFM powder;
0.5-2 parts of bifidobacterium lactis B420 powder;
1-5 parts of bifidobacterium adolescentis BA02 bacteria powder;
1-3 parts of lactobacillus reuteri SD5865 bacteria powder;
stachyose 30-60 parts;
30-60 parts of inulin;
10-30 parts of fructo-oligosaccharide;
10-20 parts of pumpkin powder;
30-80 parts of resistant dextrin; and
silica, 0.2-1 part.
A method for preparing a probiotic composition with the functions of improving insulin resistance and fasting blood glucose comprises the steps of weighing selected components according to the selected weight portions, and then uniformly mixing.
Further, in the preparation method, the selected food raw materials and/or auxiliary materials or additives are weighed according to the selected weight portions and mixed uniformly;
weighing all the bacterial powders according to the selected weight portions and uniformly mixing to obtain a bacterial powder mixture;
mixing the above mixture of food materials and/or adjuvants or additives with the fungus powder mixture.
Further, in the preparation method, inulin and pumpkin powder are weighed and uniformly mixed and recorded as a material A;
respectively weighing stachyose, fructo-oligosaccharide, partial resistant dextrin and silicon dioxide for standby;
weighing stachyose and mixing with part of resistant dextrin, and uniformly mixing to obtain material B;
respectively weighing fructo-oligosaccharide and silicon dioxide for later use;
weighing the rest of resistant dextrin, lactobacillus MN-Gup, lactobacillus acidophilus NCFM, lactobacillus B420, bifidobacterium adolescentis BA02 and lactobacillus reuteri SD5865 powder, mixing uniformly and marking as a material C;
and uniformly mixing the material A, the material B, the material C, fructo-oligosaccharide and silicon dioxide.
The technical scheme of the invention has the following advantages:
1. the invention provides a probiotic composition for improving insulin resistance and fasting blood glucose, which comprises the following components: bifidobacterium lactis (Bifidobacterium lactis) MN-Gup, lactobacillus acidophilus (Lactobacillus acidophilus) NCFM, bifidobacterium lactis (Bifidobacterium lactis) B420, bifidobacterium adolescentis (Bifidobacterium adolescentis) BA02 and lactobacillus reuteri (Lactobacillus ruteri) SD5865; the applicant finds that compared with normal people, bifidobacteria in the intestinal tracts of adult diabetics are obviously fewer, and particularly the bifidobacteria of adolescents are obvious; the invention relates to a method for reducing harmful bacteria and endotoxin in human bodies, restoring barrier effect of intestinal walls, relieving inflammatory reaction, further restoring normal action of insulin, achieving the effects of reducing insulin resistance index and reducing fasting blood glucose, and further relieving diabetes.
2. The invention provides a probiotic composition for improving insulin resistance and fasting blood glucose, which comprises the following components: the weight portions are as follows: 1-50 parts of bifidobacterium lactis MN-Gup bacterial powder; 1-50 parts of lactobacillus acidophilus NCFM powder; 0.1-20 parts of bifidobacterium lactis B420 powder; 1-50 parts of bifidobacterium adolescentis BA02 bacteria powder; and 0.1-30 parts of lactobacillus reuteri SD5865 bacteria powder; in the proportion, the bifidobacterium lactis MN-Gup, the lactobacillus acidophilus NCFM, the bifidobacterium lactis B420, the bifidobacterium adolescentis BA02 and the lactobacillus reuteri SD5865 have strong synergistic effect, and the effects of reducing insulin resistance index and reducing fasting blood glucose are more remarkable.
3. The probiotic composition with the functions of improving insulin resistance and fasting blood glucose provided by the invention further comprises food raw materials, wherein the food raw materials are at least one selected from stachyose, inulin, fructo-oligosaccharide, pumpkin powder and resistant dextrin, and the food raw materials can promote proliferation of bifidobacterium lactis MN-Gup, lactobacillus acidophilus NCFM, bifidobacterium lactis B420, bifidobacterium adolescentis BA02 and lactobacillus reuteri SD5865 on one hand and can have a certain auxiliary effect on blood glucose and insulin reaction on the other hand.
4. The invention provides a probiotic composition for improving insulin resistance and fasting blood glucose, which comprises the following components in parts by weight: 1-5 parts of bifidobacterium lactis MN-Gup bacterial powder; 1-5 parts of lactobacillus acidophilus NCFM powder; 0.5-2 parts of bifidobacterium lactis B420 powder; 1-5 parts of bifidobacterium adolescentis BA02 bacteria powder; 1-3 parts of lactobacillus reuteri SD5865 bacteria powder; stachyose 30-60 parts; 30-60 parts of inulin; 10-30 parts of fructo-oligosaccharide; 10-20 parts of pumpkin powder; 30-80 parts of resistant dextrin; and 0.2 to 1 part of silicon dioxide; the composition has proper proportion, can directly depend on the effect of 5 probiotics in intestinal tracts, reduces in-vivo inflammation, improves insulin sensitivity and promotes blood sugar metabolism, thereby reducing fasting blood sugar and achieving the effect of assisting in reducing blood sugar.
5. According to the preparation method of the probiotic composition for improving insulin resistance and fasting blood glucose, provided by the invention, as the water content of pumpkin powder is high, inulin with a relatively high formula and a relatively low water content is selected to be mixed with the pumpkin powder, and the mixture is uniformly recorded as material A; mixing stachyose with higher water content with part of resistant dextrin, and uniformly mixing to obtain material B; the rest fructo-oligosaccharides and silicon dioxide with lower water content are respectively weighed for standby; because the weight of the bacterial powder is very light, the rest of resistant dextrin is required to be weighed and mixed with the bacterial powder of the bifidobacterium lactis MN-Gup, the lactobacillus acidophilus NCFM, the bifidobacterium lactis B420, the bifidobacterium adolescentis BA02 and the lactobacillus reuteri SD5865, so that the mixing uniformity of the formula is improved, and the evenly mixed premix is marked as a C material; and uniformly mixing the material A, the material B, the material C, fructo-oligosaccharide and silicon dioxide.
Detailed Description
The following examples are provided for a better understanding of the present invention and are not limited to the preferred embodiments described herein, but are not intended to limit the scope of the invention, any product which is the same or similar to the present invention, whether in light of the present teachings or in combination with other prior art features, falls within the scope of the present invention.
The specific experimental procedures or conditions are not noted in the examples and may be followed by the operations or conditions of conventional experimental procedures described in the literature in this field. The reagents or apparatus used were conventional reagent products commercially available without the manufacturer's knowledge.
The bifidobacterium lactis MN-Gup bacterial powder in the following examples is prepared according to a conventional method, and the method can be as follows:
and (3) taking a seed freezing tube of bifidobacterium lactis MN-Gup, activating and continuously activating the seed freezing tube in a culture medium of MRS+0.05% L-cysteine hydrochloride for three generations at 37 ℃, inoculating a starter to a fermentation tank containing the culture medium of MRS+0.05% L-cysteine hydrochloride for fermentation, centrifuging the seed freezing tube by a disc centrifuge after fermentation is finished, collecting thalli, mixing the thalli with a protective agent containing trehalose, emulsifying the mixture, freeze-drying the mixture in a freeze-drying machine, and crushing the mixture to finally obtain bacterial powder, wherein the viable count of the bacterial powder is more than or equal to 5000 hundred million CFU/g.
The lactobacillus acidophilus NCFM powder, the bifidobacterium lactis B420 powder, the bifidobacterium adolescentis BA02 powder and the lactobacillus reuteri SD5865 powder, the lactobacillus plantarum powder, the food raw materials and the auxiliary materials or the additives are all commercial products.
The animal bifidobacterium subspecies (Bifidobacterium animalis subsp.lactis) Mn-Gup are preserved in China general microbiological culture Collection center (CGMCC) No.15578, and the preservation date is 2018, 4 months and 10 days. In the present invention, bifidobacterium animalis subspecies (Bifidobacterium animalis subsp.lacti) MN-Gup, abbreviated as bifidobacterium lactis MN-Gup.
Lactobacillus acidophilus NCFM powder (viable count is greater than or equal to 2000 hundred million CFU/g), bifidobacterium lactis B420 powder (viable count is greater than or equal to 4000 hundred million CFU/g), bifidobacterium adolescentis BA02 powder (viable count is greater than or equal to 1000 hundred million CFU/g) and Lactobacillus reuteri SD5865 powder (viable count is greater than or equal to 3500 hundred million CFU/g), all of which are purchased from DuPont Dannike, italy probiotics and Swedish Probi. Lactobacillus plantarum powder (viable count is more than or equal to 1000 hundred million CFU/g).
Example 1
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 1 below:
table 1 probiotic composition formulation
Example 2
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 2 below:
table 2 probiotic composition formulation
Example 3
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 3 below:
table 3 probiotic composition formulation
Example 4
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 4 below:
table 4 probiotic composition formulation
Example 5
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 5 below:
table 5 probiotic composition formulation
Example 6
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 6 below:
TABLE 6 probiotic composition formulation
Example 7
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 7 below:
TABLE 7 probiotic composition formulation
Example 8
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 8 below:
table 8, probiotic composition formulation
Example 9
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 9 below:
table 9, probiotic composition formulation
Example 10
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 10 below:
table 10 probiotic composition formulation
Example 11
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 11 below:
table 11 probiotic composition formulation
Example 12
The present example provides a probiotic composition with improved insulin resistance and fasting glucose, formulated as shown in table 12 below:
table 12, probiotic composition formulation
Example 13
This example provides a method of preparing the probiotic composition of example 1 with improved insulin resistance and fasting glucose comprising the steps of:
(1) Weighing inulin and pumpkin powder according to the formula amount, uniformly mixing and marking as a material A;
(2) Weighing stachyose and partial resistant dextrin according to the formula amount, mixing uniformly, and calculating as material B for later use;
(3) Weighing fructo-oligosaccharide and silicon dioxide according to the formula amount for standby
(4) Weighing the rest part of resistant dextrin, and the formula amount of the Bifidobacterium lactis MN-Gup powder, the Lactobacillus acidophilus NCFM powder, the Bifidobacterium lactis B420 powder, the Bifidobacterium adolescentis BA02 powder and the Lactobacillus reuteri SD5865 powder, uniformly mixing and marking as a C material;
(5) And uniformly mixing the material A, the material B, the material C, fructo-oligosaccharide and silicon dioxide.
Example 14
The preparation method of the probiotic composition with the function of improving insulin resistance and fasting blood glucose of examples 2-5 and examples 8-12 is the same as that of example 13.
Example 15
This example provides a method of preparing the probiotic composition of example 6 with improved insulin resistance and fasting glucose comprising the steps of: weighing lactobacillus bifidus MN-Gup bacterial powder, lactobacillus acidophilus NCFM bacterial powder, lactobacillus bifidus B420 bacterial powder, bifidobacterium adolescentis BA02 bacterial powder, lactobacillus reuteri SD5865 bacterial powder and resistant dextrin according to the formula amount, and uniformly mixing.
Example 16
This example provides the same method of preparation of the probiotic composition of example 7 with improved insulin resistance and fasting glycemia as example 15.
Comparative example
The probiotic compositions of comparative examples 1-6 with improved insulin resistance and fasting glucose were formulated as follows in tables 13-14:
table 13 probiotic composition formulation
Table 14, probiotic composition formulation
The preparation method of the probiotic composition with improved insulin resistance and fasting blood glucose of comparative examples 1-6 is different from example 13 only in that in step (4), the remaining part of resistant dextrin and the formulation amount of bifidobacterium lactis MN-Gup powder, lactobacillus acidophilus NCFM powder, bifidobacterium lactis B420 powder, bifidobacterium adolescentis BA02 powder, lactobacillus reuteri SD5865 powder and/or lactobacillus plantarum powder are weighed and uniformly mixed and recorded as a material C.
Experimental example 1
1. And (3) test design:
the detection basis is as follows: an accessory 3 auxiliary blood sugar reducing function evaluation method for national food and medicine supervision [ 2012 ];
test animals:
SPF SD rats, 160+ -20 g, males, were adapted to the environment for 5 days after purchase, and normal animal hypoglycemic experiments were performed.
High heat energy feed: 10% of lard, 15% of sucrose, 15% of egg yolk powder, 5% of casein, 1.2% of cholesterol, 0.2% of sodium cholate, 0.6% of calcium bicarbonate, 0.4% of stone powder and 52.6% of mouse maintenance material.
Test article: the probiotic compositions of examples 1, 2, 6 and comparative examples 1-6 were formulated as shown in Table 14 below, and the test samples were administered to rats at a dose of 10ml/kg BW (wherein the test samples of example 2 and example 6 were each 2.68g in 10ml deionized water, and the test samples of the other groups were each 1.34g in 10ml deionized water)
Table 14 formulation of test article
Experimental grouping:
hypoglycemic model hypoglycemic experiment: test substances are respectively given to the set experiment groups, 1 blank control group and 1 model control group, deionized water is given to the model control group, and the experiment groups and the model control group are respectively given to the stomach according to 10 mL/kg.BW for continuous stomach infusion for 33d.
2. The experimental method comprises the following steps:
hyperglycemia model blood glucose reduction experiment (tetraoxypyrimidine induces insulin resistance glucose/lipid metabolism disorder model)
The molding method comprises the following steps: rats were fed with normal maintenance feed for 5d, fasted for 3-4h, tail blood was taken, and the blood glucose levels before glucose administration (i.e., 0 h) were measured, and the blood glucose levels after 2.5g/kg BW glucose administration were 0.5 and 2h as the base values for the animals in the batch. Blood glucose levels were grouped at 0h, 0.5h, i.e., blank, model, and experimental groups of 15 animals each. The blank control group was not treated, the experimental group was given the test sample, the model control group was given the same volume of deionized water, and 33d were continued. The groups were fed with maintenance feed, after 1 week the model control group and the experimental group were changed for high-heat feed, after 3 weeks of feeding, the model control group and the experimental group were fasted for 24 hours (without water inhibition), and were given a intraperitoneal injection of 103-105 mg/kg.BW of tetraoxypyrimidine with an injection amount of 1ml/100g body weight. The high heat energy feed is fed for 3-5 days after injection. At the end of the test, animals of each group were fasted for 3-4 hours and tested for fasting blood glucose, glucose tolerance, serum insulin and cholesterol, and triglyceride levels.
Test index-fasting blood glucose and glucose tolerance
Animals in each group were fasted for 3-4h, blood glucose values immediately before glucose administration (0 h) were measured for fasting blood glucose, probiotic compositions of different formulations and dosages were administered to test samples, model control groups were administered with the same volume of deionized water, blank control groups were not treated, glucose was orally administered for 15-20min to each group for 2.5 g/kg.BW, and blood glucose values of 0.5 and 2h were measured for each group after glucose administration.
Measurement index-cholesterol, triglyceride
Each group of animals is fasted for 3-4 hours, serum cholesterol and triglyceride are detected, if the serum cholesterol or triglyceride of the model control group is obviously increased, compared with the blank control group, the difference is obvious, and the establishment of the model lipid metabolism disorder is judged, and on the basis, the blood lipid change of the model control group and the tested sample group is observed.
Measurement index insulin
Animals in each group are fasted for 3-4 hours, serum insulin is detected, and compared with a blank control group, the insulin resistance index of the model control group is not obviously reduced. The insulin resistance of the model control group and the test sample group was observed.
Evaluation index: one of the two indexes of fasting blood glucose and glucose tolerance is positive, blood fat (total cholesterol and triglyceride) is not obviously increased, and the test result of the animal with the auxiliary blood glucose reducing function of the tested sample can be judged to be positive without influencing the fasting blood glucose of normal animals. The efficacy of the composition after dry prognosis was further evaluated.
3. Experimental results
(1) Fasting blood sugar
Results for fasting blood glucose as shown in table 15, example 2 group was 2-fold dose of example 1 group, which significantly reduced fasting blood glucose and was also significantly better than the example 1 group. From this, the probiotic composition of example 2 has a certain auxiliary hypoglycemic effect as a whole, and is a preferred formulation.
Table 15 pre-and post-experimental fasting glycemia in hyperglycemic model rats
* P <0.05 compared to model control, P <0.01 compared to model control.
(2) Triglyceride (TG)
The results of Triglyceride (TG) are shown in table 16 below, and example 2 group was 2-fold dose of example 1 group, which significantly reduced triglyceride levels and was also significantly better than the example 1 group. From this, the probiotic composition of example 2 has a certain triglyceride reducing effect as a whole, and is a preferable formulation.
TABLE 16 influence of post-experimental serum CHO, TG (X+ -SD) in hyperglycemic model rats
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* P <0.05 compared to model control, P <0.01 compared to model control.
(3) Insulin resistance
The results of insulin resistance are shown in table 17 below, and the probiotic composition of example 2 was able to significantly lower the insulin resistance index, and was also significantly better than the probiotic composition of example 1. From these results, the probiotic composition of example 2 had a certain improvement in insulin resistance and insulin sensitivity, and was favorable for improving blood glucose level.
TABLE 17 post-experimental insulin levels and insulin resistance index for hyperglycemia model rats
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* P <0.05 compared to model control, P <0.01 compared to model control.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (8)
1. A probiotic composition for improving insulin resistance and fasting blood glucose, which is characterized by comprising a fungus powder mixture and food raw materials;
the fungus powder mixture consists of the following components in parts by weight:
1-50 parts of bifidobacterium animalis subspecies (Bifidobacterium animalis subsp.lactis) MN-Gup bacterial powder;
lactobacillus acidophilus (Lactobacillus acidophilus) NCFM powder 1-50 parts;
0.1-20 parts of bifidobacterium lactis (Bifidobacterium lactis) B420 bacteria powder;
1-50 parts of bifidobacterium adolescentis (Bifidobacterium adolescentis) BA02 bacteria powder; and
0.1-30 parts of lactobacillus reuteri (Lactobacillus ruteri) SD5865 bacteria powder;
the preservation number of the animal bifidobacterium subspecies (Bifidobacterium animalis subsp.lactis) MN-Gup is CGMCC No.15578;
the food material is at least one selected from stachyose, inulin, fructo-oligosaccharide, pumpkin powder and resistant dextrin.
2. The probiotic composition according to claim 1, further comprising a food or a medically acceptable adjuvant.
3. The probiotic composition according to claim 2, characterized in that the auxiliary material comprises silica.
4. A probiotic composition according to any one of claims 1 to 3, further comprising the following components in parts by weight:
stachyose, 0-100 parts;
inulin, 0-80 parts;
0-80 parts of fructo-oligosaccharide;
0-50 parts of pumpkin powder;
0-100 parts of resistant dextrin; and
silica, 0-10 parts.
5. A probiotic composition according to any one of claims 1 to 3, characterized by comprising the following components in parts by weight:
1-5 parts of bifidobacterium animalis milk subspecies MN-Gup bacterial powder;
1-5 parts of lactobacillus acidophilus NCFM powder;
0.5-2 parts of bifidobacterium lactis B420 powder;
1-5 parts of bifidobacterium adolescentis BA02 bacteria powder;
1-3 parts of lactobacillus reuteri SD5865 bacteria powder;
stachyose 30-60 parts;
30-60 parts of inulin;
10-30 parts of fructo-oligosaccharide;
10-20 parts of pumpkin powder;
30-80 parts of resistant dextrin; and
silica, 0.2-1 part.
6. A method of preparing a probiotic composition according to any one of claims 1 to 5, having an effect of improving insulin resistance and fasting glycemia, comprising weighing selected components in selected parts by weight and mixing them homogeneously.
7. The method of manufacturing according to claim 6, comprising:
weighing the selected food raw materials and auxiliary materials according to the selected weight portions, and uniformly mixing;
weighing all the bacterial powders according to the selected weight portions and uniformly mixing to obtain a bacterial powder mixture;
and uniformly mixing the mixture of the food raw materials and the auxiliary materials with the fungus powder mixture.
8. The method of manufacturing according to claim 7, comprising:
weighing inulin and pumpkin powder, uniformly mixing and marking as a material A;
weighing stachyose and mixing with part of resistant dextrin, and uniformly mixing to obtain material B;
respectively weighing fructo-oligosaccharide and silicon dioxide for later use;
weighing the rest of resistant dextrin, mixing with animal bifidobacterium milk subspecies MN-Gup, lactobacillus acidophilus NCFM, bifidobacterium lactis B420, bifidobacterium adolescentis BA02 and lactobacillus reuteri SD5865 powder, and marking the evenly mixed premix as a material C;
and uniformly mixing the material A, the material B, the material C, fructo-oligosaccharide and silicon dioxide.
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