CN113197092B - Gas-liquid circulation cuttage culture rack - Google Patents

Gas-liquid circulation cuttage culture rack Download PDF

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Publication number
CN113197092B
CN113197092B CN202110345846.9A CN202110345846A CN113197092B CN 113197092 B CN113197092 B CN 113197092B CN 202110345846 A CN202110345846 A CN 202110345846A CN 113197092 B CN113197092 B CN 113197092B
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liquid
pipe
water
culture
circulation
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CN113197092A (en
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邱昌朋
李梦珂
郭鹏靖
周忠莉
韩振海
许雪峰
王忆
张新忠
吴婷
李威
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China Agricultural University
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China Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Hydroponics (AREA)

Abstract

The invention discloses a gas-liquid circulation cuttage cultivation frame, which comprises a tissue culture frame, a culture solution circulation system and a gas supply circulation system, wherein the tissue culture frame is provided with a plurality of layers of water tanks, and each layer of water tank is provided with at least one cuttage fixing frame; the culture solution circulating system comprises a liquid supply pipeline, a tail liquid pipeline, a liquid return pipe and a water pump; the air supply circulating system comprises an air circulating loop and an ultrasonic atomizer. The gas-liquid circulation cuttage cultivation frame disclosed by the invention can save water, fertilizer and manpower under the condition of ensuring that good growth of plants can be promoted, more efficiently provides a suitable environment for rooting growth of cuttage plants, and provides a precondition foundation for realizing automatic regulation and control of air flow rate, humidity and culture solution components through air-liquid three-dimensional circulation flow.

Description

Gas-liquid circulation cuttage culture rack
Technical Field
The invention relates to the technical field of agricultural and forestry machinery, in particular to a gas-liquid circulation cuttage culture rack.
Background
The existing plant cuttage matrix is mostly solid matrix cuttage or water culture cuttage, the occupied area is large, the matrix consumption is large, the influence of soil-borne diseases, water culture algae substances and the like is easy to cause, and the requirement of cuttage plants on the environment is difficult to meet well.
Disclosure of Invention
The invention aims to provide a gas-liquid circulation cuttage cultivation rack, which is used for solving the problems that the existing solid matrix cuttage or water cuttage occupies large area of resources and consumes a large amount of matrix, is easy to cause diseases and is difficult to control environmental conditions.
The invention provides a gas-liquid circulation cuttage culture rack, comprising: the tissue culture rack is provided with a plurality of layers of water tanks, and each layer of water tank is provided with at least one cuttage fixing rack; the culture solution circulating system comprises a liquid supply pipeline, a tail liquid pipeline, a liquid return pipe and a water pump, wherein the liquid supply pipeline comprises a plurality of layers of water tanks and a plurality of liquid guide pipes, one liquid guide pipe is arranged between every two adjacent layers of the water tanks, and the lower ends of the liquid guide pipes are suspended above the adjacent layers of the water tanks; a liquid inlet of the liquid return pipe is provided with a water pump and is communicated with the tail liquid pipeline, and a liquid outlet of the liquid return pipe is communicated with the liquid supply pipeline to form a circulating pipeline; and the air supply circulation system comprises an air circulation loop and an ultrasonic atomizer, wherein the air circulation loop is respectively communicated with the space above each layer of water tank, and the ultrasonic atomizer is arranged in the water tank.
Preferably, the liquid supply pipeline further comprises a culture liquid reservoir and a liquid outlet pipe, and the culture liquid reservoir is communicated with the topmost water tank through the liquid outlet pipe; the tail liquid pipeline comprises a tail liquid pipe and a temporary liquid storage tank, the temporary liquid storage tank is communicated with the culture liquid reservoir through the liquid return pipe, and the water tank at the bottommost layer is communicated with the temporary liquid storage tank through the tail liquid pipe.
Preferably, the air circulation loop includes left tuber pipe and right tuber pipe, left tuber pipe with right tuber pipe all includes a plurality of layers of bellmouth of intercommunication each other, the bellmouth of each layer of left tuber pipe with the bellmouth of the corresponding layer of right tuber pipe is just to the both ends of the basin on corresponding layer respectively.
Preferably, a heating copper pipe is arranged in the culture solution reservoir.
Preferably, a water valve is arranged on the liquid outlet pipe.
Preferably, a water level controller is provided in the temporary tank.
Preferably, a filtering and purifying device is arranged on the tail liquid pipe.
Preferably, a fan is arranged in the wind circulation loop, and the fan is arranged behind the ultrasonic atomizer capable of generating the circulating flow of the mist.
Compared with the prior art, the invention has the beneficial effects that:
according to the gas-liquid circulation cuttage culture rack disclosed by the invention, a culture solution circulation system can realize circulation supply of water, nutrient solution and the like, an air supply circulation system can realize circulation supply in a gas form, the solution and the gas respectively provide nutrients and moisture required by growth for root systems and leaves of plants, gas-liquid phase double high-efficiency three-dimensional circulation utilization of gas-liquid form water and fertilizer in an integrated manner in a cuttage culture process is realized, water, fertilizer and manpower are saved under the condition that good growth of the plants is promoted, and a suitable environment is provided for rooting growth of the cuttage plants more efficiently; through the three-dimensional circulation flow of gas and liquid, a precondition foundation is provided for realizing the automatic regulation and control of the air flow rate, the humidity and the components of the culture solution, and the problems that the existing solid substrate cuttage or water cuttage occupies more land resources and consumes more substrates, is easy to cause diseases and is difficult to control the environmental conditions are solved.
Drawings
Fig. 1 is a schematic structural diagram of a gas-liquid circulation cuttage cultivation rack provided in embodiment 1 of the present invention.
Detailed Description
Example 1
Example 1 provides a gas-liquid circulation cutting culture shelf, the structure of which is described in detail below.
Referring to fig. 1, the gas-liquid circulation cutting culture shelf includes:
the tissue culture frame 1 is provided with a plurality of layers of water tanks 23, and each layer of water tank 23 is provided with at least one cuttage fixing frame 11;
culture solution circulation system, including liquid supply pipe way, tail liquid pipeline, return pipe 5 and water pump 51, return pipe 5's inlet be equipped with water pump 51 and with tail liquid pipeline intercommunication, return pipe 5's liquid outlet and liquid supply pipe communicate with each other to form circulation pipeline.
Specifically, the liquid supply pipeline comprises a culture liquid reservoir 21, a liquid outlet pipe 22, a plurality of layers of water tanks 23 and a liquid guide pipe 24, preferably, the cuttage fixing frame 11 is arranged right above the corresponding layer of water tank 23, and the culture liquid reservoir 21 is communicated with the topmost layer of water tank 23 through the liquid outlet pipe 22; a liquid guide pipe 24 is arranged between two water tanks 23 of each adjacent layer, and the lower end of the liquid guide pipe 24 is suspended above the water tank 23 of the next adjacent layer.
And the air supply circulating system comprises an air circulating loop and an ultrasonic atomizer 40, the air circulating loop is respectively communicated with the space above each layer of water tank 23, and the ultrasonic atomizer 40 is arranged in the topmost layer of water tank 23.
Specifically, the tail liquid line includes a tail liquid pipe 31 and a temporary liquid tank 33, the temporary liquid tank 33 communicates with the culture liquid reservoir 21 through the liquid return pipe 5, and the lowermost water tank 23 communicates with the temporary liquid tank 33 through the tail liquid pipe 31.
In general, the temporary liquid tank 33 is located at a low position and the culture liquid reservoir 21 is located at a high position, and a water pump 51 is provided to pump the culture liquid in the temporary liquid tank 33 to the culture liquid reservoir 21.
Specifically, the air circulation loop includes a left air duct 41 and a right air duct 42, and the left air duct 41 and the right air duct 42 each include a plurality of layers of bell mouths that are communicated with each other.
The bell mouth of each layer of the left air duct 41 and the bell mouth of the corresponding layer of the right air duct 42 are respectively opposite to the two ends of the water tank 23 of the corresponding layer. Specifically, for example: the bell mouth of the topmost layer of the left air duct 41 and the bell mouth of the topmost layer of the right air duct 42 are respectively right opposite to two ends of the topmost water tank 23, and the bell mouth of the bottommost layer of the left air duct 41 and the bell mouth of the bottommost layer of the right air duct 42 are respectively right opposite to two ends of the bottommost water tank 23.
The ultrasonic atomizer 40 is disposed in the topmost water tank 23 and is 5 cm lower than the liquid level of the water tank 23 in a steady state.
The tissue culture shelf 1 is used as a basic framework of the gas-liquid circulation cutting culture shelf, preferably has two or more layers, and is sleeved with a thin film with good light transmittance to form a sealed environment, so that the circulation flow of system gas is facilitated, and the evaporation and the loss of moisture are reduced;
a water tank 23 for storing a culture solution required for plant growth, which is input from the culture solution reservoir 21 through the liquid outlet pipe 22, wherein the culture solution in the water tank 23 can realize the flow of the culture solution in the water tank 23 by setting the height difference of two sides;
a heating copper pipe is arranged in the culture solution reservoir 21, the temperature of the culture solution can be adjusted and even the overall temperature of the circulating system can be adjusted by heating the culture solution, and the culture solution reservoir 21 is used for storing the culture solution and adjusting and controlling the temperature of the culture solution;
the liquid return pipe 5 and the liquid outlet pipe 22 are respectively used for inputting and outputting culture liquid to the culture liquid storage 21;
the ultrasonic atomizer 40 is used for atomizing the culture solution in the water tank 23 to provide nutrients for the plant leaves;
the cutting fixing frame 11 is a movable cutting fixing frame and provides cutting hole sites for plants;
and the liquid guide pipe 24 is arranged between the two adjacent water tanks 23 and is used as a water guide device between the two adjacent water tanks 23, and when the water level of the culture solution in the upper water tank 23 rises to the joint of the water tank 23 and the liquid guide pipe 24, the liquid guide pipe 24 guides the culture solution to the lower water tank 23.
In order to control the liquid phase circulation rate of the whole apparatus by adjusting the injection rate of the culture solution in the culture solution reservoir 21 into the water tank 23, a water valve 25 is provided on the liquid outlet pipe 22.
A water level controller 34 is further provided in the temporary liquid storage tank 33 in order to detect and control a change in the water level of the temporary liquid storage tank 33. The water level controller 34 is a mature prior art, and the specific structure can be referred to in chinese patent application No. 201920897034.3, namely, "a plant cultivation system and device and a water level controller thereof", and in chinese patent application No. 201920231960.7, "a water level controller for a humidifying tank", and the like. The water level controller 34 cooperates with the water valve 25 to realize the transfer of the culture solution from the temporary liquid tank 33 to the culture solution reservoir 21.
In order to purify the tail liquid discharged from the tail liquid pipe 31, a filtration purification device 32 is provided in the tail liquid pipe 31. The filtering and purifying device 32 is also a mature prior art, and can filter solid impurities such as blades and roots and remove non-solid impurities, thereby playing a role in purifying water quality.
The temporary liquid storage tank 33 is connected to the filtration purification apparatus 32 and the culture liquid reservoir 21, and serves as a container for temporarily storing the circulating culture liquid, and can be replenished with water and fertilizer, added with a sterilizing substance, and the like, thereby achieving the purpose of renewing and reusing the culture liquid.
In order to accelerate and regulate the gas flow in the wind circulation loop, a fan 6 is arranged in the wind circulation loop, the fan 6 is arranged behind the ultrasonic atomizer 40 which can generate the fog circulation flow, the fan 6 can accelerate the atomizing gas to flow along the wind circulation loop to realize the circulation of the atomizing gas in the wind circulation loop, and the gas circulation rate can be controlled by regulating and controlling the rotating speed of the fan 6. In addition, the air flow of the system under the action of the fan 6 eliminates the resistance of the interface layer of the blade, and is beneficial to the blade to carry out photosynthesis more efficiently.
In addition, this gas-liquid circulation cuttage cultivates frame still includes temperature sensor and humidity transducer for detect environment's temperature and humidity. The water tank 23 of each layer is provided with a cutting fixing frame 11 which can be bound with a rubber band to further fix cutting plants. The inner wall of the water tank 23 can be covered with a layer of black film, so that algae growth on the inner wall and the plant root system can be effectively prevented. The lower part of the liquid guide pipe 24 is wrapped by cotton cloth, so that the liquid guide pipe is fully contacted with air under the action of wind force, and the dissolved oxygen of the culture solution is improved.
The working principle of culture solution circulation is as follows:
opening the water valve 25, conveying the culture solution in the culture solution reservoir 21 to the topmost water tank 23 through the liquid outlet pipe 22, absorbing part of the culture solution by the growth of the plants on the topmost cutting fixing frame 11, and when the water level of the culture solution rises to the joint of the liquid guide pipe 24 and the water tank 23, allowing the redundant culture solution to flow into the next highest water tank 23 from the topmost water tank 23 through the liquid guide pipe 24 and be absorbed by the growth of the plants on the next highest cutting fixing frame 11;
the culture solution is surplus from the water tank 23 of the upper layer by layer and flows into the water tank 23 of the adjacent lower layer through the liquid guide pipe 24, and meanwhile, the culture solution is absorbed by the plant growth of the corresponding layer until the culture solution flows into the water tank 23 of the bottommost layer;
the water level of the culture solution in the bottommost water tank 23 rises to the joint of the liquid guide pipe 24 and the water tank 23, the culture solution flows into the filtering and purifying device 32 through the tail solution pipe 31, and the purified culture solution flows into the temporary liquid storage tank 33;
the liquid level of the temporary liquid storage tank 33 is higher than the steady state liquid level, the water pump 51 is turned on, and the culture liquid in the temporary liquid storage tank 33 is returned to the culture liquid reservoir 21 until the liquid level of the temporary liquid storage tank 33 is lowered to the steady state liquid level, thereby forming a circulation.
Other technical effects have the advantages that:
1. the circulation of the culture solution can realize the dual recycling of gas, liquid water and fertilizer in the cutting culture process.
2. The atomizing gas and the liquid in the water tank respectively provide nutrients and water required by plant growth for the leaves and the root systems. The required substances for plant growth are supplied to the plant leaves and the root systems in the forms of gas and liquid respectively, and meanwhile, the control of the humidity of the leaves is realized, and the full absorption of the plants to nutrient substances is promoted.
Growth substances required by gas-liquid circulation cuttage cultivation plants are supplied to the root system and the blades through culture solution in the water tank and atomizing gas in the air channel respectively, and water and fertilizer integration and gas-liquid simultaneous application are achieved; the culture solution is atomized into gas by an ultrasonic atomization device in the topmost water tank, the atomized gas circularly flows among layers by the pressurization of a fan and the connection of an air pipe, so that water and nutrient substances are provided for plant leaves, and the photosynthesis of the leaves is improved by proper wind speed; the culture solution in the water tank is communicated layer by utilizing the height difference of the liquid level, the cotton cloth at the joint of each layer is fully contacted with the air under the action of wind power to improve the dissolved oxygen amount, the culture solution flowing out of the water tank at the bottom layer needs to flow through a filtering and purifying device to remove impurities and harmful components and enters a temporary liquid storage tank, water, fertilizer and sterilizing substances can be added into the temporary liquid storage tank to adjust the culture solution, the updated culture solution is transported to a heatable water storage device by a water level controller and a water pump, and the speed of water injection into the water tank and the flow speed in the water tank are controlled by adjusting a valve of the water storage device, so that the purification cycle of the culture solution is realized; the cuttage mount is mobilizable, can adjust the position of placing on the basin in a flexible way, utilizes the rubber band fixed cuttage plant simultaneously, is convenient for get the mechanical damage of putting the operation and avoiding this process to the plant simultaneously.
In addition, this cuttage device compares with traditional solid matrix cuttage and water planting cuttage, has the advantage that space is intensive, the management is brief, the resource saving: semi-automatic control is realized through device setting, and cutting medium does not need to be replaced; the water fertilizer and the sterilizing substances are recycled, so that the environment is saved and the environment is protected; the three-dimensional cultivation makes full use of space, and can be applied to indoor cuttage, plant water culture and batch management.
In addition, the automation control of the device can be realized by adding a temperature and humidity sensor and a regulating element and setting parameters such as the temperature of the culture solution, the air humidity, the rotating speed of the fan, the rotating speed of the water pump and the like.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (5)

1. The utility model provides a frame is cultivateed in cuttage of gas-liquid circulation, its characterized in that includes:
the tissue culture rack (1) is provided with a plurality of layers of water tanks (23), and each layer of water tank (23) is provided with at least one cuttage fixing rack (11);
the culture solution circulating system comprises a liquid supply pipeline, a tail liquid pipeline, a liquid return pipe (5) and a water pump (51), wherein the liquid supply pipeline comprises a culture solution storage device (21), a liquid outlet pipe (22), a plurality of layers of water tanks (23) and a plurality of liquid guide pipes (24), and the culture solution storage device (21) is communicated with the topmost layer of water tanks (23) through the liquid outlet pipe (22); a liquid guide pipe (24) is arranged between two water tanks (23) of each adjacent layer, and the lower end of the liquid guide pipe (24) is hung above the water tank (23) of the adjacent next layer; the tail liquid pipeline comprises a tail liquid pipe (31) and a temporary liquid storage tank (33), the temporary liquid storage tank (33) is communicated with the culture liquid reservoir (21) through the liquid return pipe (5), and the water tank (23) at the bottommost layer is communicated with the temporary liquid storage tank (33) through the tail liquid pipe (31); a water pump (51) is arranged at a liquid inlet of the liquid return pipe (5) and is communicated with the temporary liquid storage tank (33), and a liquid outlet of the liquid return pipe (5) is communicated with the liquid supply pipeline to form a circulating pipeline;
the air supply circulation system comprises an air circulation loop and an ultrasonic atomizer (40), wherein the air circulation loop comprises a left air pipe (41), a right air pipe (42) and a fan (6), the left air pipe (41) and the right air pipe (42) respectively comprise a plurality of layers of horn mouths which are mutually communicated, and the horn mouth of each layer of the left air pipe (41) and the horn mouth of the corresponding layer of the right air pipe (42) are respectively right opposite to the two ends of the space above the water tank (23) of the corresponding layer; the ultrasonic atomizer (40) is arranged in the water tank (23); and the fan (6) is arranged behind the ultrasonic atomizer (40) which can generate the circulating flow of the fog.
2. The gas-liquid circulation cutting culture shelf as set forth in claim 1, wherein a heating copper pipe is provided in the culture liquid reservoir (21).
3. The gas-liquid circulation cutting culture shelf as set forth in claim 1, wherein the liquid outlet pipe (22) is provided with a water valve (25).
4. The gas-liquid circulation cutting culture rack according to claim 1, wherein a water level controller (34) is arranged in the temporary liquid storage tank (33).
5. The gas-liquid circulation cutting culture rack of claim 1,
and a filtering and purifying device (32) is arranged on the tail liquid pipe (31).
CN202110345846.9A 2021-03-31 2021-03-31 Gas-liquid circulation cuttage culture rack Active CN113197092B (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202385595U (en) * 2011-11-15 2012-08-22 江苏省农业科学院观光农业研究中心 Multilayered pipeline type ultrasonic-atomization culturing device
CN103262783A (en) * 2013-05-23 2013-08-28 北京市土肥工作站 Three-dimensional soil-less cultivating device

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202385595U (en) * 2011-11-15 2012-08-22 江苏省农业科学院观光农业研究中心 Multilayered pipeline type ultrasonic-atomization culturing device
CN103262783A (en) * 2013-05-23 2013-08-28 北京市土肥工作站 Three-dimensional soil-less cultivating device

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