CN1131889C - 质粒 - Google Patents
质粒 Download PDFInfo
- Publication number
- CN1131889C CN1131889C CN97190500A CN97190500A CN1131889C CN 1131889 C CN1131889 C CN 1131889C CN 97190500 A CN97190500 A CN 97190500A CN 97190500 A CN97190500 A CN 97190500A CN 1131889 C CN1131889 C CN 1131889C
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- Prior art keywords
- gene
- plasmid
- expression
- plasmid vector
- dna
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
- C12N9/1007—Methyltransferases (general) (2.1.1.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
菌株 | 基因型 | 起源/参考文献 |
MC4100 | F-,araD139,Δ(argF-lac)U169,thiA,rpsL150,relAl,flbB5301,deoCl,ptsF25,rbsR | Casadaban等,分子生物学杂志104(1984)541-555 |
MRi7 | MC4100Δrbs-7 | Lopilato等.细菌学杂志.158(1984)665-673 |
MRi80 | MRi7 pcnB80 | Lopilato等.分子和普通微生物学.205(1986)285-290 |
菌株 | β-半乳糖苷酶活性 | ||
质粒 | |||
pMSP6F | pMSP6L | pMS434 | |
MC4100 | 202 | 242 | 30 |
MRi7 | 234 | 287 | 23 |
MRi80 | 17 | 27 | 4 |
菌株 | β-半乳糖苷酶活性(非诱导/诱导) | |||||
pMSP6F | pMSP6L | pMS434 | ||||
非诱导 | 诱导 | 非诱导 | 诱导 | 非诱导 | 诱导 | |
MC4100(pFSP6) | 221 | 4875 | 234 | 5256 | 25 | 19 |
MRi7(pFSP6) | 220 | 3958 | 242 | 4243 | 25 | 21 |
MRi80(pFSP6) | 17 | 425 | 30 | 970 | 3 | ND |
菌株 | β-半乳糖苷酶活性(非诱导条件) | |||
pMSP6F | pMSP6L | pMSP60 | pMS434 | |
MC4100 | 221 | 234 | 30 | 25 |
Claims (22)
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8580196 | 1996-03-13 | ||
JP85801/1996 | 1996-03-13 | ||
JP85801/96 | 1996-03-13 | ||
JP208897/1996 | 1996-07-18 | ||
JP20889796 | 1996-07-18 | ||
JP208897/96 | 1996-07-18 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1193354A CN1193354A (zh) | 1998-09-16 |
CN1131889C true CN1131889C (zh) | 2003-12-24 |
Family
ID=26426808
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN97190500A Expired - Fee Related CN1131889C (zh) | 1996-03-13 | 1997-03-10 | 质粒 |
Country Status (8)
Country | Link |
---|---|
US (2) | US6165749A (zh) |
EP (1) | EP0859057B1 (zh) |
JP (2) | JP3549210B2 (zh) |
KR (1) | KR100374672B1 (zh) |
CN (1) | CN1131889C (zh) |
AU (1) | AU2233397A (zh) |
DE (1) | DE69733540T2 (zh) |
WO (1) | WO1997034006A1 (zh) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6569669B1 (en) * | 2000-10-12 | 2003-05-27 | New England Biolabs, Inc. | Host strain for low uninduced expression of foreign RNA polymerase genes |
GB0029539D0 (en) * | 2000-12-04 | 2001-01-17 | Isis Innovation | Method for identifying modulatorss of transcription |
US7794971B1 (en) | 2003-07-01 | 2010-09-14 | Epicentre Technologies Corporation | Compositions and methods for controlling copy number for a broad range of plasmids and uses thereof |
US20070202082A1 (en) * | 2005-11-09 | 2007-08-30 | Dong-Yan Jin | Promoters for RNA interference |
CN102174554A (zh) * | 2011-01-24 | 2011-09-07 | 内蒙古民族大学 | 双控双调节原核表达载体***及其构建方法和用途 |
CN111378609A (zh) * | 2018-12-27 | 2020-07-07 | 江苏金斯瑞生物科技有限公司 | 诱导质粒拷贝数增加的培养基及其应用 |
CN111705071B (zh) * | 2020-06-16 | 2023-03-10 | 西北农林科技大学 | 一种人工设计质粒pM5500及其在DNA marker制备中的应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5137823A (en) * | 1986-06-06 | 1992-08-11 | New England Biolabs, Inc. | Method for producing the bamhi restriction endonuclease and methylase |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8308236D0 (en) * | 1983-03-25 | 1983-05-05 | Celltech Ltd | Dna vectors |
GB8407498D0 (en) * | 1984-03-22 | 1984-05-02 | Biogen Nv | High copy number expression vectors |
US5354680A (en) * | 1986-06-06 | 1994-10-11 | New England Biolabs, Inc. | Method for producing the DdeI restriction endonuclease and methylase |
JP2518051B2 (ja) * | 1989-06-06 | 1996-07-24 | 東洋紡績株式会社 | AccI制限エンドヌクレア―ゼの製造方法 |
US5196318A (en) * | 1990-06-26 | 1993-03-23 | The Texas A&M University System | Precisely regulated expression of deleterious genes |
US5843703A (en) * | 1993-01-22 | 1998-12-01 | California Institute Of Technology | Enhanced production of toxic polypeptides in prokaryotes |
EP0832255B1 (en) * | 1995-06-07 | 2005-12-14 | Washington University | Recombinant bacterial system with environmentally limited viability |
JP3007584B2 (ja) | 1997-01-31 | 2000-02-07 | 文化シヤッター株式会社 | 耐火スクリーン装置 |
-
1997
- 1997-03-10 KR KR1019970708102A patent/KR100374672B1/ko not_active IP Right Cessation
- 1997-03-10 DE DE69733540T patent/DE69733540T2/de not_active Expired - Fee Related
- 1997-03-10 WO PCT/JP1997/000748 patent/WO1997034006A1/ja active IP Right Grant
- 1997-03-10 US US08/952,089 patent/US6165749A/en not_active Expired - Fee Related
- 1997-03-10 AU AU22333/97A patent/AU2233397A/en not_active Abandoned
- 1997-03-10 CN CN97190500A patent/CN1131889C/zh not_active Expired - Fee Related
- 1997-03-10 EP EP97905474A patent/EP0859057B1/en not_active Expired - Lifetime
- 1997-03-10 JP JP53243097A patent/JP3549210B2/ja not_active Expired - Fee Related
-
2000
- 2000-04-20 JP JP2000120124A patent/JP2000325094A/ja active Pending
- 2000-10-18 US US09/690,885 patent/US6770481B1/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5137823A (en) * | 1986-06-06 | 1992-08-11 | New England Biolabs, Inc. | Method for producing the bamhi restriction endonuclease and methylase |
Also Published As
Publication number | Publication date |
---|---|
EP0859057A1 (en) | 1998-08-19 |
JP2000325094A (ja) | 2000-11-28 |
US6770481B1 (en) | 2004-08-03 |
JP3549210B2 (ja) | 2004-08-04 |
AU2233397A (en) | 1997-10-01 |
KR100374672B1 (ko) | 2003-12-18 |
US6165749A (en) | 2000-12-26 |
EP0859057B1 (en) | 2005-06-15 |
DE69733540D1 (en) | 2005-07-21 |
CN1193354A (zh) | 1998-09-16 |
DE69733540T2 (de) | 2006-05-11 |
WO1997034006A1 (fr) | 1997-09-18 |
EP0859057A4 (en) | 2003-03-19 |
KR19990014759A (ko) | 1999-02-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: TAKARA BIO INC. Free format text: FORMER OWNER: PO CO., LTD. Effective date: 20040827 |
|
C41 | Transfer of patent application or patent right or utility model | ||
C56 | Change in the name or address of the patentee |
Owner name: PO CO., LTD. Free format text: FORMER NAME OR ADDRESS: TAKARA SHUZO CO., LTD. |
|
CP01 | Change in the name or title of a patent holder |
Address after: Kyoto City, Kyoto, Japan Patentee after: Treasure Limited by Share Ltd. Address before: Kyoto City, Kyoto, Japan Patentee before: TAKARA SHUZO Co.,Ltd. |
|
TR01 | Transfer of patent right |
Effective date of registration: 20040827 Address after: The Japanese city of Tianjin Patentee after: TAKARA BIO Inc. Address before: Kyoto City, Kyoto, Japan Patentee before: Treasure Limited by Share Ltd. |
|
CI01 | Publication of corrected invention patent application |
Correction item: Alteration of the patent owner and his address Correct: Takara Holdings Inc. False: Treasure Limited by Share Ltd Number: 40 Page: 559 Volume: 20 |
|
ERR | Gazette correction |
Free format text: CORRECT: PATENTEE AND ADDRESS CHANGES; FROM: PO CO., LTD. TO: BAO HOLDINGS LIMITED |
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C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |