CN113185563A - Method for purifying inosine from fermentation liquor - Google Patents
Method for purifying inosine from fermentation liquor Download PDFInfo
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- CN113185563A CN113185563A CN202110453871.9A CN202110453871A CN113185563A CN 113185563 A CN113185563 A CN 113185563A CN 202110453871 A CN202110453871 A CN 202110453871A CN 113185563 A CN113185563 A CN 113185563A
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- inosine
- fermentation liquor
- filtrate
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/167—Purine radicals with ribosyl as the saccharide radical
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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- Life Sciences & Earth Sciences (AREA)
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- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention provides a method for purifying inosine from fermentation liquor, and relates to the technical field of chemical industry. The method for purifying inosine from the fermentation liquor comprises the following steps: s1, selecting inosine fermentation liquor for treatment; s2, placing the inosine fermentation liquor filtered in the S1 into a reaction kettle; s3, independently storing the filtrate collected in the S2; s4, concentrating, crystallizing and drying the inosine water solution filtered in the S2; s5, filtering the filtrate in the step S2 by using a microfiltration membrane; and S6, purifying the filtrate filtered out in the S5 and the wastewater collected in the S3 by using a sewage treatment device. Through collecting inosine zymotic fluid filtrating glycogen, purify the emission through filtrating sewage treatment's mode simultaneously, make environmental protection pressure little, dilute while filterable setting through using the nanofiltration membrane to inosine zymotic fluid, make harvesting that inosine can the maximize, be worth promoting greatly.
Description
Technical Field
The invention relates to the technical field of chemical engineering, in particular to a method for purifying inosine from fermentation liquor.
Background
Inosine is also called inosine, etc., is a nucleoside compound formed by combining hypoxanthine and ribose, inosinic acid can be used as a precursor for synthesizing adenylic acid and guanylic acid in the de novo synthesis of purine, is suitable for leucopenia, thrombocytopenia and various heart diseases caused by various reasons, inosine can also be used as an accessory enzyme medicament, inosine is a component of ATP, coenzyme A, ribonucleic acid and deoxyribonucleic acid in the body and is involved in the metabolism and energy metabolism of the body, can improve the level of ATP in the body, can be converted into various nucleotides and is involved in the synthesis of protein, inosine has good permeability on cell membranes, can directly enter cells, can be converted into nucleotides and further is converted into ATP to be involved in the metabolism, has the effects of promoting the recovery of liver cells and preventing fatty liver, can improve the activity of various enzymes and stimulate the production of antibodies in the body, the conventional extraction process of inosine needs to be carried out by steps of fermentation liquor, column chromatography, concentration, recrystallization and the like, and the steps can also be carried out by steps of fermentation liquor, flocculation, precipitation or other methods for separating partial sugar residues, concentration, recrystallization and the like.
The conventional process for purifying inosine has three obvious defects, one is that a large amount of waste water or other waste materials are generated, the environmental protection pressure is high, the two concentration processes are long, the energy consumption is large, the energy conservation and consumption reduction are not facilitated, and the product yield is not ideal.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a method for purifying inosine from fermentation liquor, and solves the problems of large environmental protection pressure of a large amount of waste materials generated by inosine purification by a conventional process and low yield of high-yield products with long working procedure and energy consumption.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a method for purifying inosine from a fermentation broth, comprising the following steps:
s1, selecting inosine fermentation liquor for treatment, adding sodium hydroxide into the inosine fermentation liquor to adjust the pH value of the inosine fermentation liquor to 6-8 during treatment, then stirring the inosine fermentation liquor by using a stirring device, heating the inosine fermentation liquor to 115 ℃ during stirring, filtering the inosine fermentation liquor by using a filtering device after stirring for 1-3 minutes, and filtering to remove solids and impurities in the inosine fermentation liquor;
s2, placing the inosine fermentation liquor filtered in the S1 into a reaction kettle, adding tap water with the same volume as that of the inosine fermentation liquor into the reaction kettle, passing the diluted inosine fermentation liquor through a nanofiltration membrane by using a pump body, slowly introducing the tap water into the inosine fermentation liquor by using another new pump body when the pump body transports the inosine fermentation liquor, introducing clear water into the inosine fermentation liquor by using the other pump body, wherein the clear water accounts for one third of the volume of the inosine fermentation liquor transported by the pump body, diluting the inosine in a concentrated solution by using water, avoiding the blockage of the nanofiltration membrane due to the overhigh concentration of residual liquid on the surface of the nanofiltration membrane, allowing the nanofiltration membrane to pass through the tap water and the inosine, collecting and then reserving the filtered inosine fermentation liquor, and separately collecting the redundant filtrate in the inosine fermentation liquor;
s3, independently storing the filtrate collected in S2, and reversely flushing the nanofiltration membrane from the inside of the nanofiltration membrane by using tap water through a pump body, so that a little residual substance for filtering inosine on the surface of the nanofiltration membrane is flushed and removed, and the flushed wastewater is collected;
s4, concentrating and crystallizing the inosine water solution filtered in the step S2, pumping the crystallized mixture into a drying device for drying by using a pump after crystallization, and collecting inosine powder after drying at the temperature during drying;
s5, filtering the filtrate in the step S2 by using a microfiltration membrane, collecting glycogen after filtration for secondary culture of inosine fermentation liquor, and separately collecting the filtrate left after filtration;
s6, purifying the filtrate filtered out in the S5 and the wastewater collected in the S3 by using a sewage treatment device, allowing the filtrate to be subjected to standing purification by spherical sludge particles during purification, wherein the standing time is 1-2 days, and then discharging the purified wastewater.
Preferably, the drying equipment in S4 is a spray drying device.
Preferably, the microfiltration membrane in S5 can only filter out glycogen in the filtrate.
Preferably, the spherical sludge granules in S6 are made of activated carbon, and the sewage treatment microorganisms are attached to the spherical sludge granules.
Preferably, the filtering device in S1 is a seaweed filter.
Preferably, the nanofiltration membranes in the S2 and the S3 are both tubular.
(III) advantageous effects
The invention provides a method for purifying inosine from fermentation liquor. The method has the following beneficial effects:
1. according to the invention, the inosine fermentation liquor filtrate is filtered again by the microfiltration membrane, so that redundant glycogen in the inosine fermentation liquor can be collected, the glycogen can be collected, purified raw materials can be saved, the environmental pollution is reduced, and meanwhile, the filtrate is purified and discharged in a sewage treatment mode, so that the environmental protection pressure is low, and the environment is protected.
2. According to the invention, the inosine fermentation liquor is diluted and filtered by using the nanofiltration membrane, so that the yield of inosine can reach the highest, and meanwhile, inosine is directly collected, so that the process of inosine purification is greatly shortened, the energy consumption is reduced, the yield of purification is improved, the purification cost is reduced, the surface of the nanofiltration membrane is not adhered and blocked due to overhigh concentration of filtrate due to the dilution and filtration, the use efficiency of the nanofiltration membrane is improved, and the method is worthy of vigorous popularization.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
the embodiment of the invention provides a method for purifying inosine from fermentation liquor, which comprises the following steps:
s1, selecting inosine fermentation liquor for treatment, adding sodium hydroxide into the inosine fermentation liquor to adjust the pH value to 6 during treatment, adjusting acid and alkali to ensure that the inosine fermentation liquor is convenient to extract, stirring the inosine fermentation liquor by using a stirring device, heating the inosine fermentation liquor to 110 ℃ during stirring, sterilizing the inosine fermentation liquor by heating, stirring for 2 minutes to uniformly mix the inosine fermentation liquor so as to separate impurities such as organic matters or solid particles in the inosine fermentation liquor, filtering the inosine fermentation liquor by using a filtering device, filtering the solid and the impurities in the inosine fermentation liquor, and protecting a nanofiltration membrane during next filtering of the inosine fermentation liquor;
s2, placing the inosine fermentation liquor filtered in the S1 into a reaction kettle, adding tap water with the same volume as the inosine fermentation liquor into the reaction kettle, passing the diluted inosine fermentation liquor through a nanofiltration membrane by using a pump body, slowly introducing tap water into the inosine fermentation liquor by using another new pump body when the inosine fermentation liquor is transported by the pump body, introducing clear water into the inosine fermentation liquor by using the other pump body, wherein the clear water accounts for one third of the volume of the inosine fermentation liquor transported by the pump body, diluting the inosine in a concentrated solution by using water, avoiding the blockage of the nanofiltration membrane due to the overhigh concentration of residual liquid on the surface of the nanofiltration membrane, allowing the nanofiltration membrane to pass only the tap water and the inosine, collecting and reserving the filtrate for later use, and separately collecting the redundant filtrate in the inosine fermentation liquor, wherein the arrangement of filtering and diluting inosine simultaneously can not only maximize the filtration of inosine, but also can prevent the filtrate after the filtration of inosine from adhering to the surface of the nanofiltration membrane due to overhigh concentration, the trouble of one-time treatment and one-time cleaning of the nanofiltration membrane is avoided, and the nanofiltration membrane can be used well after inosine is filtered by the nanofiltration membrane through backwashing of the nanofiltration membrane, so that the cleaning is convenient;
s3, storing the filtrate collected in S2 independently, reversely flushing the nanofiltration membrane from the inside of the nanofiltration membrane by the tap water through the pump body, flushing a few residual substances for filtering inosine on the surface of the nanofiltration membrane, collecting the flushed wastewater, and enabling the nanofiltration membrane to be normally used by means of backwashing, so that the trouble of cleaning and disassembling is avoided;
s4, concentrating and crystallizing the inosine water solution filtered by the S2, pumping a crystallization mixture into a drying device for drying by using a pump after crystallization, collecting inosine powder after drying at the temperature during drying, concentrating and crystallizing after inosine is purified, which belongs to the prior art and is not described herein any more, wherein the drying device can effectively collect inosine, and the inosine is obtained by crystallizing and concentrating immediately after a nanofiltration membrane filters the inosine, so that the process steps are greatly shortened;
s5, filtering the filtrate in the S2 by using a microfiltration membrane, collecting glycogen after filtering for secondary culture of inosine fermentation broth, and collecting the filtrate left after filtering, wherein the glycogen can be reused and can be used as a culture medium raw material in the next fermentation of the inosine strain, so that the environmental protection pressure can be reduced when the filtrate is discharged;
s6, purifying the filtrate filtered in the S5 and the wastewater collected in the S3 by using a sewage treatment device, allowing the filtrate to be subjected to standing purification through spherical sludge particles during purification for 1 day, and then discharging the purified wastewater, wherein the filtrate can meet the environmental protection requirement through the arrangement of sewage purification, so that the sewage treatment pressure is low.
The drying equipment in S4 is a spray drying device, and the spray drying efficiency is high.
The microfiltration membrane in S5 can only filter out glycogen in the filtrate, and the glycogen can be filtered by the microfiltration membrane to filter out impurities such as sewage.
The spherical sludge granules in the S6 are made of activated carbon, sewage treatment microorganisms are attached to the spherical sludge granules, and the microorganisms can decompose organic matters in the filtrate well, so that the filtrate can meet the requirement of environment-friendly sewage discharge.
The filtering means in S1 is a seaweed filter, which filters out impurities well.
The nanofiltration membranes in the S2 and the S3 are both tubular, so that inosine is well filtered, and backwashing of the nanofiltration membranes is facilitated.
Example two:
the embodiment of the invention provides a method for purifying inosine from fermentation liquor, which comprises the following steps:
s1, selecting inosine fermentation liquor for treatment, adding sodium hydroxide into the inosine fermentation liquor to adjust the pH value of the inosine fermentation liquor to 8 during treatment, then stirring the inosine fermentation liquor by using a stirring device, heating the inosine fermentation liquor to 115 ℃ during stirring, filtering the inosine fermentation liquor by using a filtering device after stirring for 3 minutes, and filtering out solids and impurities in the inosine fermentation liquor;
s2, placing the inosine fermentation liquor filtered in the S1 into a reaction kettle, adding tap water with the same volume as that of the inosine fermentation liquor into the reaction kettle, passing the diluted inosine fermentation liquor through a nanofiltration membrane by using a pump body, slowly introducing the tap water into the inosine fermentation liquor by using another new pump body when the pump body transports the inosine fermentation liquor, introducing clear water into the inosine fermentation liquor by using the other pump body, wherein the clear water accounts for one third of the volume of the inosine fermentation liquor transported by the pump body, diluting the inosine in a concentrated solution by using water, avoiding the blockage of the nanofiltration membrane due to the overhigh concentration of residual liquid on the surface of the nanofiltration membrane, allowing the nanofiltration membrane to pass through the tap water and the inosine, collecting and then reserving the filtered inosine fermentation liquor, and separately collecting the redundant filtrate in the inosine fermentation liquor;
s3, independently storing the filtrate collected in S2, and reversely flushing the nanofiltration membrane from the inside of the nanofiltration membrane by using tap water through a pump body, so that a little residual substance for filtering inosine on the surface of the nanofiltration membrane is flushed and removed, and the flushed wastewater is collected;
s4, concentrating and crystallizing the inosine water solution filtered in the step S2, pumping the crystallized mixture into a drying device for drying by using a pump after crystallization, and collecting inosine powder after drying at the temperature during drying;
s5, filtering the filtrate in the step S2 by using a microfiltration membrane, collecting glycogen after filtration for secondary culture of inosine fermentation liquor, and separately collecting the filtrate left after filtration;
and S6, purifying the filtrate filtered out in the S5 and the wastewater collected in the S3 by using a sewage treatment device, allowing the filtrate to be subjected to standing purification by spherical sludge particles for 2 days during purification, and then discharging the purified wastewater.
The drying apparatus in S4 is a spray drying device.
The microfiltration membrane in S5 can only filter out glycogen in the filtrate.
The spherical sludge granules in S6 are made of activated carbon, and sewage-treating microorganisms are attached to the spherical sludge granules.
The filtering device in S1 is a seaweed filter.
The nanofiltration membranes in the S2 and the S3 are both tubular.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A method for purifying inosine from fermentation liquor is characterized in that: the method comprises the following steps:
s1, selecting inosine fermentation liquor for treatment, adding sodium hydroxide into the inosine fermentation liquor to adjust the pH value of the inosine fermentation liquor to 6-8 during treatment, then stirring the inosine fermentation liquor by using a stirring device, heating the inosine fermentation liquor to 115 ℃ during stirring, filtering the inosine fermentation liquor by using a filtering device after stirring for 1-3 minutes, and filtering to remove solids and impurities in the inosine fermentation liquor;
s2, placing the inosine fermentation liquor filtered in the S1 into a reaction kettle, adding tap water with the same volume as that of the inosine fermentation liquor into the reaction kettle, passing the diluted inosine fermentation liquor through a nanofiltration membrane by using a pump body, slowly introducing tap water into the inosine fermentation liquor by using another pump body when the pump body transports the inosine fermentation liquor, wherein the amount of clear water introduced into the inosine fermentation liquor by using the other pump body accounts for one third of the volume of the inosine fermentation liquor transported by the pump body, diluting inosine in a concentrated solution by using water, avoiding the blockage of the nanofiltration membrane due to the overhigh concentration of residual liquid on the surface of the nanofiltration membrane, allowing the nanofiltration membrane to pass through the tap water and the inosine, collecting the inosine fermentation liquor for later use, and separately collecting the redundant filtrate in the inosine fermentation liquor;
s3, independently storing the filtrate collected in S2, and reversely flushing the nanofiltration membrane from the inside of the nanofiltration membrane by using tap water through a pump body, so that a little residual substance for filtering inosine on the surface of the nanofiltration membrane is flushed and removed, and the flushed wastewater is collected;
s4, concentrating and crystallizing the inosine water solution filtered in the step S2, pumping the crystallized mixture into a drying device for drying by using a pump after crystallization, and collecting inosine powder after drying at the temperature during drying;
s5, filtering the filtrate in the step S2 by using a microfiltration membrane, collecting glycogen after filtration for secondary culture of inosine fermentation liquor, and separately collecting the filtrate left after filtration;
s6, purifying the filtrate filtered out in the S5 and the wastewater collected in the S3 by using a sewage treatment device, allowing the filtrate to be subjected to standing purification by spherical sludge particles during purification, wherein the standing time is 1-2 days, and then discharging the purified wastewater.
2. The method for purifying inosine from a fermentation broth according to claim 1, wherein: the drying equipment in S4 is a spray drying device.
3. The method for purifying inosine from a fermentation broth according to claim 1, wherein: the microfiltration membrane in S5 can only filter out glycogen in the filtrate.
4. The method for purifying inosine from a fermentation broth according to claim 1, wherein: the spherical sludge granules in the S6 are made of activated carbon, and sewage treatment microorganisms are attached to the spherical sludge granules.
5. The method for purifying inosine from a fermentation broth according to claim 1, wherein: the filtering device in the S1 is a seaweed filter.
6. The method for purifying inosine from a fermentation broth according to claim 1, wherein: the nanofiltration membranes in the S2 and the S3 are both tubular.
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| CN202110453871.9A CN113185563A (en) | 2021-04-26 | 2021-04-26 | Method for purifying inosine from fermentation liquor |
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| CN202110453871.9A CN113185563A (en) | 2021-04-26 | 2021-04-26 | Method for purifying inosine from fermentation liquor |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1056897A (en) * | 1990-05-26 | 1991-12-11 | 宜昌市第四制药厂 | Counter-current to remove bacteria, one absorption and membrane sepn extract the inosine method |
| CN101948439A (en) * | 2010-08-17 | 2011-01-19 | 刘平 | Extraction method and application to medicine of active alkaloid compounds in cervus nippon temminck |
| CN102639722A (en) * | 2008-12-09 | 2012-08-15 | 东丽株式会社 | Method for producing sugar liquid |
| CN105688499A (en) * | 2016-03-16 | 2016-06-22 | 昆山东大智汇技术咨询有限公司 | Membrane method extraction refining technology of inosine fermentation liquor |
-
2021
- 2021-04-26 CN CN202110453871.9A patent/CN113185563A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1056897A (en) * | 1990-05-26 | 1991-12-11 | 宜昌市第四制药厂 | Counter-current to remove bacteria, one absorption and membrane sepn extract the inosine method |
| CN102639722A (en) * | 2008-12-09 | 2012-08-15 | 东丽株式会社 | Method for producing sugar liquid |
| CN101948439A (en) * | 2010-08-17 | 2011-01-19 | 刘平 | Extraction method and application to medicine of active alkaloid compounds in cervus nippon temminck |
| CN105688499A (en) * | 2016-03-16 | 2016-06-22 | 昆山东大智汇技术咨询有限公司 | Membrane method extraction refining technology of inosine fermentation liquor |
Non-Patent Citations (1)
| Title |
|---|
| 金超楠: "拜氏梭菌发酵条件的优化", 《大连工业大学硕士学位论文》, 16 June 2015 (2015-06-16), pages 18 * |
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Application publication date: 20210730 |