CN113180050B - 胡椒碱作为农业杀菌剂的用途 - Google Patents
胡椒碱作为农业杀菌剂的用途 Download PDFInfo
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- 239000003899 bactericide agent Substances 0.000 title claims abstract description 15
- MXXWOMGUGJBKIW-YPCIICBESA-N piperine Chemical compound C=1C=C2OCOC2=CC=1/C=C/C=C/C(=O)N1CCCCC1 MXXWOMGUGJBKIW-YPCIICBESA-N 0.000 title claims abstract description 15
- 235000019100 piperine Nutrition 0.000 title claims abstract description 14
- 229940075559 piperine Drugs 0.000 title claims abstract description 14
- WVWHRXVVAYXKDE-UHFFFAOYSA-N piperine Natural products O=C(C=CC=Cc1ccc2OCOc2c1)C3CCCCN3 WVWHRXVVAYXKDE-UHFFFAOYSA-N 0.000 title claims abstract description 14
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- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 3
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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- IGFHQQFPSIBGKE-UHFFFAOYSA-N Nonylphenol Natural products CCCCCCCCCC1=CC=C(O)C=C1 IGFHQQFPSIBGKE-UHFFFAOYSA-N 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
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- 241000607479 Yersinia pestis Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
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- 230000009036 growth inhibition Effects 0.000 description 1
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- 230000002401 inhibitory effect Effects 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
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- DPCSPGOPQYRPCP-UHFFFAOYSA-N n-[4-[4-[3-(dimethylamino)propyl]piperazin-1-yl]phenyl]acetamide Chemical compound C1CN(CCCN(C)C)CCN1C1=CC=C(NC(C)=O)C=C1 DPCSPGOPQYRPCP-UHFFFAOYSA-N 0.000 description 1
- SNQQPOLDUKLAAF-UHFFFAOYSA-N nonylphenol Chemical compound CCCCCCCCCC1=CC=CC=C1O SNQQPOLDUKLAAF-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229950004889 piperamide Drugs 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 1
- 229920005552 sodium lignosulfonate Polymers 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/04—Sulfonic acids; Derivatives thereof
- A01N41/08—Sulfonic acid halides; alpha-Hydroxy-sulfonic acids; Amino-sulfonic acids; Thiosulfonic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明公开了化合物胡椒碱作为农业杀菌剂的用途。该化合物作为杀菌剂用于防治农业真菌性病害,可有效地防治菌核病、纹枯病、灰霉病、赤霉病、炭疽病、恶苗病、蔓枯病、枯萎病、黄萎病等农作物病害。胡椒碱作为活性成分制成的农业杀菌剂具有防治效果好,广谱性及低毒、无残留、无污染、无公害的特点,特别有利于环境保护。
Description
技术领域
本发明属于农业技术领域,具体涉及一种化合物胡椒碱作为活性成分制备农业杀菌剂的用途。
背景技术
农作物的病害由于不像害虫和杂草那样容易被人们及时察觉,所以往往造成防治上的忽视和困难,因而其危害就更为严重。据报道,全世界单是由病原真菌引起的植物病害就多达一万种,所造成的损失占作物年度总损失的10%~30%。如果把病毒、细菌和线虫等引起的植物病害也算在内,其损失就更为可观。
为了满足人们对粮食和其它生活物质日益增长的需要,不得不采用化学品以保护作物不受有害生物的侵袭,其中杀菌剂起着重要作用。具有农用杀菌活性的天然产物,一般易被植物吸收和传导,对植物药害小或无药害,因此倍受重视。
化合物胡椒碱,别名:胡椒酰胺、胡椒辣碱,英文名: Piperine,英文别名: 1-Piperylpiperidine,CAS号: 94-62-2,分子式: C17H19NO3,分子量: 285.33,分子结构:
该化合物溶于甲醇、乙醇、丙酮、乙酸乙酯、氯仿等有机溶剂。对其抑制植物病原菌的活性作用研究较少。
发明内容
本发明的目的是探索化合物胡椒碱作为活性成分在制备农业杀菌剂的应用。
本发明通过大量研究证明,此化合物作为活性成分制备得到的农业杀菌剂具有优异的杀菌活性,可应用在防治各种农作物上的真菌性病害,抑菌普较广,如:菌核病、纹枯病、灰霉病、赤霉病、炭疽病、恶苗病、蔓枯病、枯萎病、黄萎病。
化合物胡椒碱可从天然植物中分离纯化得到,亦可通过化学合成方法得到。其作为活性成分和农药助剂、赋型剂配制成农药上允许的任意一种剂型。其还可以和其它农药复配,与农药助剂、赋型剂混合制成农药上允许的任意一种剂型。所述的剂型可以是乳油、悬浮剂、水乳剂、可湿性粉剂、微乳剂或水分散颗粒剂等。所用的助剂可以采用十二烷基苯磺酸钙、吡咯烷酮、聚氧乙烯聚氧丙稀嵌段化合物、壬基酚聚氧乙烯醚等,赋形剂可为环己酮、溶剂油、溶剂油、水、丙二醇、去离子水等。复配所用的其他农药可为有机杀菌剂类,如***类杀菌剂***醇;植物源杀菌剂,如大蒜素、蛇床子素等。
与现有技术比较本发明的有益效果:化合物胡椒碱作为杀菌剂,抑菌谱广,持效期长,是农业上理想的杀菌剂。与一般化学农药相比,由于来源于天然植物的提取物,对于各种粮食、瓜果、蔬菜等经济作物具有低毒、无残留、无污染、无公害的特点,更有利环境保护。
具体实施方式
以下通过具体实施方式对本发明效果做进一步说明:
实施例1:化合物胡椒碱对农业病害的防治
供试菌株立枯丝核菌(Thanatephorus cucumeris)从水稻纹枯病病叶上分离;核盘菌(Sclerotioia selerotiorum)从油菜菌核病菌株上分离得到;灰葡萄孢菌(Botrytis cinerea)从草莓灰霉病上分离得到;禾谷镰孢菌(Fusarium graminearum)从小麦赤霉病病株上分离得到;胶孢炭疽菌(Colletotrichum gloeosporioides)从葡萄炭疽病病株上分离得到;串珠镰孢菌(Fusarium moniliforme)从水稻恶苗病病株上分离得到。这六种菌种由南京农业大学植保学院提供。瓜类黑腐球壳菌(Didymella bryoniae )从西瓜蔓枯病病株上分离得到,稻瘟病菌(Magnaporthe oryzae)从水稻稻瘟病株上分离得到,这两种菌种由江苏省中国科学院植物研究所提供。尖孢镰刀菌(Fusarium oxysporum)从棉花枯萎病病株上分离得到,大丽轮枝菌(Verticillium dahliae) 从棉花黄萎病病株上分离得到,由江苏省农科院植保所提供。
供试化合物胡椒碱,将其溶于DMSO制成1000μg/mL母液,保存备用。PDA培养基:200g去皮马铃薯切成块,煮沸10min后,取汁,加入20g琼脂,加热至熔化,再加入20g葡萄糖,用水定容至1000mL。
采用菌丝生长速率测定法对菌丝生长的抑制测定,从PDA平板上预培养的供试菌边缘打取菌碟(直径5mm),将供试药剂母液与培养基均匀混合,使供试药剂最终浓度分别为60、50、40、30、20、10、0ppm,混合液分别倾入直径为9cm的灭菌培养皿中制成平板,每皿接种一菌碟,菌丝面朝上,每处理重复3次。25℃培养至对照菌落布满培养皿三分之二以上时,十字交叉法测量菌落增长直径。由菌落直径平均值(mm)计算生长抑制率。对所得的数据用DPS V3.01专业版软件分析处理,得出标准曲线和EC50值。
从结果中可以看出化合物胡椒碱可有效地防治菌核病、纹枯病、灰霉病、赤霉病、炭疽病、恶苗病、蔓枯病、枯萎病、黄萎病,对这几种病害抑制中浓度 EC50为19.3687~96.0871 mg/L。尤其对灰霉病、蔓枯病、炭疽病、纹枯病效果较好,对这几种病害抑制中浓度EC50均小于40mg/L,具体实验结果见表1。通过实验充分体现了本发明农药对这些菌的显著防治效果,但对稻瘟病的抑制效果不好,EC50和回归方程都无法算出。
表1 化合物对农业病害的抑菌率
植物病原菌 | Regression equation | R | EC<sub>50</sub>(mg/L) | 95%置信区间 |
灰葡萄孢菌 | y=1.8060+2.4815x | 0.9853 | 19.3687 | 17.0819~ 21.9617 |
瓜类黑腐球壳菌 | y=1.5520+2.3587x | 0.9834 | 28.9613 | 25.6683~ 32.6768 |
胶孢炭疽菌 | y=2.2854+1.7819x | 0.9521 | 33.3764 | 24.5826~ 45.3161 |
立枯丝核菌 | y=2.7985+1.3908x | 0.9740 | 38.2787 | 31.9477~ 45.8643 |
尖孢镰刀菌 | y=1.9725+1.7915x | 0.9989 | 48.9705 | 46.8245~ 51.2148 |
核盘菌 | y=1.3461+2.1066x | 0.9960 | 54.2595 | 49.3909~ 59.6081 |
禾谷镰孢菌 | y=3.4831+0.8560x | 0.9661 | 59.1725 | 43.7605~ 80.0125 |
串珠镰孢菌 | y=3.0131+1.0559x | 0.9739 | 76.1612 | 55.2824~ 104.9254 |
大丽轮枝菌 | y=0.1355+2.4535x | 0.9974 | 96.0871 | 85.9332~ 107.4406 |
实施例2:
称取该化合物与木质素、硅藻土等常规辅料按照常规工艺,制成有效成分浓度为2%的可湿性粉剂。
实施例3:
称取该化合物与十二烷基苯磺酸钙、苯乙基酚聚氧乙烯醚、溶剂油等常规辅料按照常规工艺,制成有效成分浓度为2%的乳油。
实施例4:
称取该化合物与木质磺酸钠、高岭土等常规辅料按照常规工艺,制成有效成分浓度为2%的水分散粒剂。
实施例5:
称取该化合物与溶剂油、吡咯烷酮,黄原胶等常规辅料按照常规工艺,制成有效成分浓度为1%的水乳剂。
实施例6:
称取该化合物与十二烷基苯磺酸钙、吡咯烷酮等常规辅料按照常规工艺,制成有效成分浓度为1%的微乳剂。
实施例7:
按照重量比1:5的比例称取该化合物和***醇,与溶剂油、吡咯烷酮,黄原胶等常规辅料按照常规工艺,制成有效成分浓度为5%的水乳剂。
实施例8:
按照重量比6:1的比例称取该化合物和大蒜素,与溶剂油、吡咯烷酮,黄原胶等常规辅料按照常规工艺,制成有效成分浓度为5%的水乳剂。
实施例9:
按照重量比1:1的比例称取该化合物和蛇床子素,与溶剂油、吡咯烷酮,黄原胶等常规辅料按照常规工艺,制成有效成分浓度为5%的水乳剂。
Claims (3)
1.胡椒碱作为活性成分在制备防治西瓜蔓枯病杀菌剂中的用途。
2.根据权利要求1所述的用途,其特征在于所述活性成分和农药助剂混合制成农药上允许的任意一种剂型;或者所述活性成分和其他农药以及农药助剂混合制成农药上允许的任意一种剂型。
3.根据权利要求2所述的用途,其特征在于所述的剂型是乳油、悬浮剂、水乳剂、可湿性粉剂、微乳剂或水分散颗粒剂;所述的其他农药为***类杀菌剂***醇或植物源杀菌剂大蒜素、蛇床子素。
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1813537A (zh) * | 2005-02-06 | 2006-08-09 | 陈华玲 | 胡椒碱杀虫剂及其制备方法 |
CN101218912A (zh) * | 2008-01-29 | 2008-07-16 | 杜道林 | 一种胡椒碱除虫灭菌剂 |
CN107646844A (zh) * | 2017-09-29 | 2018-02-02 | 杨凌农科大无公害农药研究服务中心 | 一种胡椒碱与丁香酚植物源复配增效杀菌剂及其应用 |
CN107711845A (zh) * | 2017-09-29 | 2018-02-23 | 杨凌农科大无公害农药研究服务中心 | 一种胡椒碱与啶酰菌胺增效复配杀菌剂及其应用 |
CN111511211A (zh) * | 2017-12-21 | 2020-08-07 | 国立比利时根特大学 | 用于控制植物病原体的化合物 |
-
2021
- 2021-04-26 CN CN202110453586.7A patent/CN113180050B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1813537A (zh) * | 2005-02-06 | 2006-08-09 | 陈华玲 | 胡椒碱杀虫剂及其制备方法 |
CN101218912A (zh) * | 2008-01-29 | 2008-07-16 | 杜道林 | 一种胡椒碱除虫灭菌剂 |
CN107646844A (zh) * | 2017-09-29 | 2018-02-02 | 杨凌农科大无公害农药研究服务中心 | 一种胡椒碱与丁香酚植物源复配增效杀菌剂及其应用 |
CN107711845A (zh) * | 2017-09-29 | 2018-02-23 | 杨凌农科大无公害农药研究服务中心 | 一种胡椒碱与啶酰菌胺增效复配杀菌剂及其应用 |
CN111511211A (zh) * | 2017-12-21 | 2020-08-07 | 国立比利时根特大学 | 用于控制植物病原体的化合物 |
Non-Patent Citations (4)
Title |
---|
Antibacterial and Antifungal Activities of easily grown Eritrean Black Pepper;Abraham Yohannes, et,al.;《International Journal of Engineering Research & Technology》;20180228;第7卷(第2期);第81-83页 * |
Fungicidal activity of pipernonaline, a piperidine alkaloid derived from long pepper, Piper longum L., against phytopathogenic fungi;Sung-Eun Lee et,al.;《Crop Protection》;20010731;第20卷(第6期);第523-528页 * |
Natural phenolic derivatives based on piperine scaffold as potential antifungal agents;Jingjing Wang et,al.;《BMC Chemistry》;20200330;第14卷(第1期);第1-12页 * |
多取代内酰胺衍生物的设计、合成及生物活性研究;王双双;《中国优秀硕士学位论文全文数据库•工程科技Ⅰ辑》;20210115(第1期);第57、67页 * |
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