CN113179775A - Melon grafted seedling growth nondestructive testing and healing environment evaluation method in healing period - Google Patents
Melon grafted seedling growth nondestructive testing and healing environment evaluation method in healing period Download PDFInfo
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- 230000035876 healing Effects 0.000 title claims abstract description 96
- 230000012010 growth Effects 0.000 title claims abstract description 62
- 241000219112 Cucumis Species 0.000 title claims abstract description 36
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 title claims abstract description 36
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 238000011156 evaluation Methods 0.000 title claims abstract description 34
- 238000009659 non-destructive testing Methods 0.000 title claims description 35
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- 230000035784 germination Effects 0.000 claims description 10
- 239000011087 paperboard Substances 0.000 claims description 10
- 239000012286 potassium permanganate Substances 0.000 claims description 10
- 238000002791 soaking Methods 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- 230000000630 rising effect Effects 0.000 claims description 8
- 230000004083 survival effect Effects 0.000 claims description 8
- 238000004590 computer program Methods 0.000 claims description 7
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- 230000000249 desinfective effect Effects 0.000 description 2
- 230000001066 destructive effect Effects 0.000 description 2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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Abstract
The invention belongs to the technical field of grafting detection and evaluation, and discloses a melon grafted seedling healing period growth nondestructive detection and healing environment evaluation method, which comprises the following steps: respectively carrying out stock seedling culture and scion seedling culture; completely flattening the first true leaf of the stock, and grafting when the first true leaf of the scion is half-unfolded; continuously detecting the growth state of the grafted seedlings; and evaluating the grafting healing environment through the change of the slope of the growth curve. The method has the advantages of simple operation, no need of large-scale equipment support, low cost, no place limitation, no need of professional technical personnel for guidance, continuous detection of the growth condition of the grafted seedling in the grafting healing period, convenience for seedling raising factories and farmers to accurately evaluate the environmental parameters in the grafting healing period, and capability of providing certain improvement suggestions.
Description
Technical Field
The invention belongs to the technical field of grafting detection and evaluation, and particularly relates to a nondestructive growth detection and healing environment evaluation method for melon grafted seedlings in a healing period.
Background
At present, the grafting can improve the crop yield, enhance the stress resistance, solve the problems of soil-borne diseases, continuous cropping obstacles and the like, and is widely applied to the production of vegetable seedlings. Environmental changes in the grafting healing period have great influence on the quality of grafted seedlings, and the evaluation of the grafting healing environment in the past usually depends on the strong seedling index and the survival rate. The measurement of the strong seedling index belongs to destructive detection, and the seedlings cannot be reused; the determination of the survival rate needs to increase repeated experiments to ensure the accuracy of the evaluation. If the growth state of the grafted seedlings in the grafting healing period can be detected nondestructively and continuously, the seedling using amount and accidental errors can be reduced, and the evaluation accuracy is ensured.
At present, large-scale instruments and equipment are needed for continuously detecting the growth of the grafted seedlings in the healing period, and professional hardware, software platforms and professional technical personnel are needed for guiding. Therefore, it is necessary to develop a technology which is simple in operation, free from large-scale equipment support, low in cost, free from site limitation, free from the guidance of professional technicians, capable of continuously and nondestructively detecting the growth state of the grafted seedlings and simultaneously providing a certain suggestion for improving the grafting healing environment.
Through the above analysis, the problems and defects of the prior art are as follows:
(1) environmental changes in the grafting healing period have great influence on the quality of grafted seedlings, the conventional evaluation on the grafting healing environment generally depends on the strong seedling index and the survival rate, the measurement of the strong seedling index belongs to destructive detection, and seedlings cannot be reused; the determination of the survival rate needs to increase repeated experiments to ensure the accuracy of the detection.
(2) The continuous detection of the growth of the grafted seedlings in the healing period needs large-scale instruments and equipment, and needs to be provided with professional hardware, software platforms and professional technical personnel for guidance.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a nondestructive testing and healing environment evaluation method for growth of melon grafted seedlings in a healing period.
The invention is realized in such a way that a melon grafted seedling growth nondestructive testing and healing environment evaluation method in the healing period comprises the following steps:
respectively carrying out stock seedling culture and scion seedling culture;
completely flattening the first true leaf of the stock, and grafting when the first true leaf of the scion is half-unfolded;
step three, continuously detecting the growth state of the grafted seedlings;
and step four, evaluating the grafting healing environment through the slope change of the growth curve.
Further, in the step one, the stock seedling raising comprises:
sterilizing the melon stock seeds with 1 per mill of potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 h; then accelerating germination for 24h at 30 ℃, sowing the radicles in 50-hole plug disks when the radicles are exposed to white, and grafting the 1 st true leaves when the leaves are completely spread.
Further, in the step one, the scion seedling raising comprises:
sterilizing melon scion seeds with 1 ‰ potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 hr; accelerating germination for 24h at 30 ℃, and broadcasting the radicles in a flat plate when the radicles are exposed to white; grafting the 1 st true leaf when the true leaf is half-unfolded.
Further, the second step comprises:
rootstock treatment, comprising:
cutting off all growth points and one cotyledon from the cotyledon base part by using a grafting knife in an inclined angle of 45 degrees, and reserving one cotyledon; the cutting of the noodles is finished as soon as possible, and the smoothness of the cutting of the noodles is guaranteed.
Scion treatment, comprising:
the grafting blade is obliquely cut upwards at an angle of 45 degrees at a position 0.5cm-1.0cm away from the base part of the cotyledon, and the tangent plane is ensured to be parallel and smooth with the stock.
Further, the matrix is thoroughly irrigated 1 day before grafting, and the grafting blade, the grafting clip and the grafting healing environment are sterilized and disinfected by 75% medical alcohol in advance, and are used after being naturally dried.
Further, in the third step, the requirements of the grafted seedling are as follows: and (4) continuously photographing a single plant, and completing a true leaf growth curve only by 5-6 grafted seedlings.
In the third step, the continuous detection of the growth state of the grafted seedling comprises:
flattening the true leaves of the grafted seedlings on a paperboard by using tweezers; photographing the mobile phone in parallel to a paperboard containing a 10mm line segment; importing the picture into a computer, and opening the picture by imagej;
calculating the area of the blade by taking a line segment of 10mm as a scale; calculating the true leaf growth area of the single plant 1-10 days after grafting, drawing a true leaf growth curve of the survival plant in different healing environments, and analyzing the rising inflection point and the change of the slope of the growth curve.
Further, in the fourth step, the evaluation of the grafting healing environment through the slope change of the growth curve comprises:
and (3) evaluating the grafting healing environment through the rising inflection point of the curve and the change of the slope of the curve: the earlier the inflection point is, the faster the slope rises, and the grafting healing environment is better.
The invention also aims to provide a nondestructive testing device for implementing the growth nondestructive testing and healing environment evaluation method for the melon grafted seedling in the healing period, wherein the nondestructive testing device comprises a hardboard with a length of 10mm, a camera or a camera for taking a picture, tweezers and a computer with imagej software.
Another object of the present invention is to provide a computer device, which includes a memory and a processor, wherein the memory stores a computer program, and the computer program, when executed by the processor, causes the processor to execute the non-destructive testing for growth and evaluation method for healing environment during healing period of melon grafting seedling.
Another object of the present invention is to provide a computer-readable storage medium, which stores a computer program, which, when executed by a processor, causes the processor to execute the method for nondestructive testing of growth of melon grafted seedlings during a healing period and evaluating a healing environment.
The invention also aims to provide an information data processing terminal which executes the growth nondestructive testing and healing environment evaluation method in the healing period of melon grafted seedlings.
By combining all the technical schemes, the invention has the advantages and positive effects that: the melon grafted seedling growth nondestructive testing and healing environment evaluation method in the healing period is simple to operate, free of large-scale equipment support, low in cost, free of place limitation, capable of continuously testing the growth condition of the grafted seedling in the grafting healing period without guidance of professional technicians, convenient for seedling raising factories and farmers to accurately evaluate environmental parameters in the grafting healing period, and capable of providing certain improvement suggestions.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of the non-destructive testing of the growth of melon grafted seedlings in the healing period and the evaluation method of the healing environment provided by the embodiment of the invention.
FIG. 2 is a photograph showing the area of the true leaf of the grafted seedling according to the embodiment of the present invention.
Fig. 3 is a schematic diagram of calculating the true leaf area by Imagej according to the embodiment of the present invention.
FIG. 4 is a schematic diagram of growth curves under different healing environments (T1, T2, T3, see in particular Table 1) provided by an embodiment of the present invention;
in the figure: t1, the healing environment is excellent; t2, good healing environment; t3, healing environment poor.
FIG. 5 is a schematic diagram of the plant growth status 10d after grafting according to the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a nondestructive testing method for growth of melon grafted seedlings in a healing period and an evaluation method for a healing environment, and the invention is described in detail below by combining the attached drawings.
As shown in fig. 1, the method for nondestructive testing of growth of melon grafted seedlings in the healing period and evaluation of the healing environment provided by the embodiment of the present invention comprises the following steps:
s101, respectively carrying out stock seedling culture and scion seedling culture;
s102, completely flattening the first true leaf of the stock, and grafting when the first true leaf of the scion is half-unfolded;
s103, continuously detecting the growth state of the grafted seedlings;
and S104, evaluating the grafting healing environment through the slope change of the growth curve.
In step S101, the stock seedling raising includes:
sterilizing the melon stock seeds with 1 per mill of potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 h; then accelerating germination for 24h at 30 ℃, sowing the radicles in 50-hole plug disks when the radicles are exposed to white, and grafting the 1 st true leaves when the leaves are completely spread.
In step S101, the scion seedling raising includes:
sterilizing melon scion seeds with 1 ‰ potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 hr; accelerating germination for 24h at 30 ℃, and broadcasting the radicles in a flat plate when the radicles are exposed to white; grafting the 1 st true leaf when the true leaf is half-unfolded.
The step S102 includes:
rootstock treatment, comprising:
cutting off all growth points and one cotyledon from the cotyledon base part by using a grafting knife in an inclined angle of 45 degrees, and reserving one cotyledon; the cutting of the noodles is finished as soon as possible, and the smoothness of the cutting of the noodles is guaranteed.
Scion treatment, comprising:
the grafting blade is obliquely cut upwards at an angle of 45 degrees at a position 0.5cm-1.0cm away from the base part of the cotyledon, and the tangent plane is ensured to be parallel and smooth with the stock.
And (3) watering the substrate thoroughly 1 day before grafting, sterilizing and disinfecting the grafting blade, the grafting clip and the grafting healing environment by 75% medical alcohol in advance, and naturally airing for use.
In step S103, the requirements of the grafted seedling are: and (4) continuously photographing a single plant, and completing a true leaf growth curve only by 5-6 grafted seedlings.
In step S103, the nondestructive testing device includes a cardboard with a length of 10mm, a camera or a camera for taking a picture, tweezers, and a computer with imagej software.
In step S103, the continuous detection of the growth state of the grafted seedling includes:
flattening the true leaves of the grafted seedlings on a paperboard by using tweezers; photographing the mobile phone in parallel to a paperboard containing a 10mm line segment; importing the picture into a computer, and opening the picture by imagej;
calculating the area of the blade by taking a line segment of 10mm as a scale; calculating the true leaf growth area of the single plant 1-10 days after grafting, drawing a true leaf growth curve of the survival plant in different healing environments, and analyzing the rising inflection point and the change of the slope of the growth curve.
In step S104, evaluating the grafting healing environment through the slope change of the growth curve includes:
and (3) evaluating the grafting healing environment through the rising inflection point of the curve and the change of the slope of the curve: the earlier the inflection point is, the faster the slope rises, and the grafting healing environment is better.
The technical solution of the present invention will be further described with reference to the following examples.
Example 1
The melon grafting seedling growth nondestructive testing and healing environment evaluation technology in the healing period provided by the embodiment of the invention comprises the following steps:
(1) stock seedling culture: sterilizing the melon stock seeds with 1 per mill of potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 h; then accelerating germination for 24h at 30 ℃, sowing the radicles in 50-hole plug disks when the radicles are exposed to white, and grafting the 1 st true leaves when the leaves are completely spread.
(2) Scion seedling culture: sterilizing melon scion seeds with 1 ‰ potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 hr; accelerating germination for 24h at 30 ℃, and broadcasting the radicles in a flat plate when the radicles are exposed to white; grafting the 1 st true leaf when the true leaf is half-unfolded.
(3) And (3) grafting period: stock: 1, completely flattening true leaves, and grafting: half-spread of the 1 st true leaf.
(4) The grafting method comprises the following steps: adopting monocotyledon grafting.
(5) Treating the rootstock: cutting off all growth points and one cotyledon from the cotyledon base part by using a grafting knife in an inclined angle of 45 degrees, and reserving one cotyledon; the cutting of the noodles is finished as soon as possible, and the smoothness of the cutting of the noodles is guaranteed. Scion treatment: the grafting blade is obliquely cut at an angle of about 45 degrees upwards at a position 0.5cm-1.0cm away from the base part of the cotyledon, and the tangent plane is ensured to be parallel and smooth with the stock. Grafting: the scion is closely attached to the grafting section of the stock, and a grafting clip is clipped.
(6) And (3) continuously detecting the growth state of the grafted seedlings:
preparing a nondestructive testing device: a cardboard (with 10mm long line), a camera or a camera for taking pictures, a pair of tweezers, and a computer with imagej software.
Nondestructive testing process: flattening the true leaves of the grafted seedlings on a paper board with tweezers → taking a picture (containing a 10mm line segment) of a mobile phone in parallel with the paper board → guiding the picture into a computer → opening the picture with imagej → taking the 10mm line segment as a scale, calculating the area of the leaves → calculating the growth area of the single true leaves 1-10d after grafting → drawing the growth curve of the true leaves of the surviving plants.
Evaluation indexes are as follows: and (3) evaluating the grafting healing environment through the rising inflection point of the curve and the change of the slope of the curve: the earlier the inflection point is, the faster the slope rises, which indicates that the grafting healing environment is better.
Requirements of grafted seedlings: and (4) continuously photographing a single plant, and completing a true leaf growth curve only by 5-6 grafted seedlings.
Note that:
the blade and the grafting clip used in the invention (4) are sterilized in advance by 75% medical alcohol and are used after being naturally dried.
In the invention (4), the matrix is watered thoroughly 1 day before grafting.
Example 2
The melon grafting seedling growth nondestructive testing and healing environment evaluation technology in the healing period provided by the embodiment of the invention comprises the following steps:
(1) stock seedling culture: sterilizing melon stock seeds 'super boxing' with 1 ‰ potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 hr; then accelerating germination for 24h at 30 ℃, sowing the radicles in 50-hole plug disks when the radicles are exposed to white, and grafting the 1 st true leaves when the leaves are completely spread.
(2) Scion seedling culture: sterilizing melon scion seeds 'scales' with 1 ‰ potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 hr; accelerating germination for 24h at 30 ℃, and broadcasting the radicles in a flat plate when the radicles are exposed to white; grafting the 1 st true leaf when the true leaf is half-unfolded. The scions are sown 3-4 days later than the rootstocks.
(3) Preparation before grafting: and (3) watering the matrix thoroughly 1 day before grafting, and sterilizing and disinfecting the grafting blade and the grafting healing environment.
(4) Grafting operation: the monocotyledon is adopted for the close-grafting,
(5) treating the rootstock: cutting off all growth points and one cotyledon from the cotyledon base part by using a grafting knife in an inclined angle of 45 degrees, and reserving one cotyledon; the cutting of the noodles is finished as soon as possible, and the smoothness of the cutting of the noodles is guaranteed. Scion treatment: the grafting blade is obliquely cut at an angle of about 45 degrees upwards at a position 0.5cm-1.0cm away from the base part of the cotyledon, and the tangent plane is ensured to be parallel and smooth with the stock. Grafting: the scion is closely attached to the grafting section of the stock, and a grafting clip is clipped.
(6) Nondestructive testing of the growth state of the grafted seedling:
6 grafted seedlings are grafted under different grafting healing environments, and the true leaf growth curve of the surviving grafted seedlings is counted.
Preparing a nondestructive testing device: a cardboard (line with 10mm length), a camera or mobile phone for taking pictures, a pair of tweezers, and a computer with imagej software
Nondestructive testing process: flattening the true leaves of the grafted seedlings on a paper board with tweezers → taking a picture (containing a 10mm line segment) with a mobile phone in parallel with the paper board → guiding the picture into a computer → opening the picture with imagej → taking the 10mm line segment as a scale, calculating the area of the leaves → calculating the growth area of the single true leaves of 1-10d after grafting → drawing true leaf growth curves of plants under different healing environments, and analyzing the rising inflection point of the growth curves and the change of the slope.
In the nondestructive testing process, the area of the true leaf is photographed, as shown in figure 2; imagej calculates the true leaf area, see fig. 3; growth curves were plotted and the growth curves for different healing environments are shown in figure 4.
The results and recommendations for different healing environments are shown in FIG. 5, tables 1-3.
TABLE 1 grafting healing Environment management parameters of T1, T2, T3
TABLE 2 evaluation accuracy and improvement advice for grafting healing environment according to the present invention (see TABLE 1 for specific environment)
TABLE 3 environmental evaluation and accuracy of the traditional seedling strengthening index and survival rate method for grafting healing (see TABLE 1 for specific environment)
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. The melon grafted seedling growth nondestructive testing and healing environment evaluation method in the healing period is characterized by comprising the following steps of:
respectively carrying out stock seedling culture and scion seedling culture;
completely flattening the first true leaf of the stock, and grafting when the first true leaf of the scion is half-unfolded;
step three, continuously detecting the growth state of the grafted seedlings;
and step four, evaluating the grafting healing environment through the slope change of the growth curve.
2. The method for nondestructive testing of growth and evaluation of healing environment of melon grafted seedling in healing period according to claim 1, wherein in the first step, the rootstock seedling raising comprises:
sterilizing the melon stock seeds with 1 per mill of potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 h; then accelerating germination for 24h at 30 ℃, sowing the radicles in 50-hole plug disks when the radicles are exposed to white, and grafting the 1 st true leaves when the leaves are completely spread.
3. The non-destructive testing and healing environment evaluating method for growth of melon grafted seedling at healing period as claimed in claim 1, wherein in step one, the scion seedling raising comprises:
sterilizing melon scion seeds with 1 ‰ potassium permanganate solution for 10min, washing with tap water for 3 times, and soaking in tap water at room temperature for 8 hr; accelerating germination for 24h at 30 ℃, and broadcasting the radicles in a flat plate when the radicles are exposed to white; grafting the 1 st true leaf when the true leaf is half-unfolded.
4. The non-destructive testing and healing environment evaluating method for growth of melon grafted seedling in healing period as claimed in claim 1, wherein in step three, the grafted seedling is: and continuously photographing the single plant, and finishing a true leaf growth curve of 5-6 grafted seedlings.
5. The method for nondestructive testing of growth and evaluation of healing environment of melon grafted seedling in healing period as claimed in claim 1, wherein in step three, the continuous testing of growth state of grafted seedling comprises:
flattening the true leaves of the grafted seedlings on a paperboard by using tweezers; photographing the mobile phone in parallel to a paperboard containing a 10mm line segment; importing the picture into a computer and opening the picture;
calculating the area of the blade by taking a line segment of 10mm as a scale; calculating the true leaf growth area of the single plant 1-10 days after grafting, drawing a true leaf growth curve of the survival plant in different healing environments, and analyzing the rising inflection point and the change of the slope of the growth curve.
6. The method for nondestructive testing of growth and evaluation of healing environment during healing period of melon graft as claimed in claim 1 wherein in step four, the evaluation of the healing environment of graft by the slope change of growth curve comprises:
and (3) evaluating the grafting healing environment through the rising inflection point of the curve and the change of the slope of the curve: the earlier the inflection point is, the faster the slope rises, and the grafting healing environment is better.
7. A nondestructive testing device for implementing the nondestructive testing of growth of melon grafted seedlings in the healing period and the evaluation method of healing environment as claimed in any one of claims 1 to 6, wherein the nondestructive testing device comprises a cardboard with a length of 10mm, a camera or a mobile phone for taking a picture, tweezers and a computer with imagej software.
8. A computer device, characterized in that the computer device comprises a memory and a processor, the memory stores a computer program, when the computer program is executed by the processor, the computer program causes the processor to execute the melon grafting seedling healing period growth nondestructive testing and healing environment evaluation method according to any one of claims 1-6.
9. A computer-readable storage medium storing a computer program which, when executed by a processor, causes the processor to execute the method for nondestructive testing of growth during a healing period of a melon graft and evaluation of healing environment of any one of claims 1 to 6.
10. An information data processing terminal, characterized in that the information data processing terminal executes the growth nondestructive testing and healing environment evaluation method for melon grafted seedling in the healing period according to any one of claims 1 to 6.
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2021
- 2021-05-25 CN CN202110573355.XA patent/CN113179775B/en not_active Expired - Fee Related
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| CN110915447A (en) * | 2019-10-01 | 2020-03-27 | 华中农业大学 | Method for growing seedlings of muskmelon stocks by cotyledon-free grafting |
| CN111066509A (en) * | 2020-01-19 | 2020-04-28 | 华中农业大学 | Method for improving quality of watermelon rootstock zero-leaf grafted seedlings |
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