CN113176328A - Method for evaluating influence of hydro-junction on fish habitat based on otolith micro-area in-situ trace elements - Google Patents
Method for evaluating influence of hydro-junction on fish habitat based on otolith micro-area in-situ trace elements Download PDFInfo
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- 230000002842 otolith Effects 0.000 title claims abstract description 77
- 210000001265 otolithic membrane Anatomy 0.000 title claims abstract description 77
- 241000356847 Otolithes Species 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 33
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 24
- 235000013619 trace mineral Nutrition 0.000 title claims abstract description 24
- 239000011573 trace mineral Substances 0.000 title claims abstract description 24
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 18
- 241001519451 Abramis brama Species 0.000 claims abstract description 27
- 238000004458 analytical method Methods 0.000 claims abstract description 11
- 230000008859 change Effects 0.000 claims abstract description 10
- 239000011347 resin Substances 0.000 claims abstract description 5
- 229920005989 resin Polymers 0.000 claims abstract description 5
- 210000003128 head Anatomy 0.000 claims abstract description 4
- 238000000608 laser ablation Methods 0.000 claims abstract description 4
- 238000000095 laser ablation inductively coupled plasma mass spectrometry Methods 0.000 claims abstract description 4
- 238000005070 sampling Methods 0.000 claims abstract description 4
- 238000012216 screening Methods 0.000 claims abstract description 4
- 230000008569 process Effects 0.000 claims description 13
- 238000012360 testing method Methods 0.000 claims description 13
- 238000012937 correction Methods 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 4
- 238000005498 polishing Methods 0.000 claims description 4
- 238000002679 ablation Methods 0.000 claims description 3
- 239000012159 carrier gas Substances 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims description 3
- 239000007789 gas Substances 0.000 claims description 3
- 239000001307 helium Substances 0.000 claims description 3
- 229910052734 helium Inorganic materials 0.000 claims description 3
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 claims description 3
- 238000010408 sweeping Methods 0.000 claims 1
- 238000010276 construction Methods 0.000 abstract description 6
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 9
- 239000013505 freshwater Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 241001275890 Megalobrama amblycephala Species 0.000 description 5
- 230000012010 growth Effects 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 230000005012 migration Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000001617 migratory effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
- G01N27/626—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode using heat to ionise a gas
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/32—Polishing; Etching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
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Abstract
The invention discloses a method for evaluating the influence of a hydro-junction on a fish habitat based on otolith micro-area in-situ trace elements, which comprises the following steps: (1) collecting samples: collecting Guangdong bream samples along the line of the hydraulic junction; (2) extracting otolith: extracting otolith from a balance sac at the head of a Guangdong bream sample and grinding the otolith; (3) making otolith slices: putting the otolith into a grinding mold, adding the prepared cold buried resin for embedding, and placing the otolith in a shade place for hardening; (4) the otolith in-situ micro-area trace element analysis method comprises the following steps: using LA-ICP-MS laser ablation sampling, and adopting a line scanning mode; (5) screening out the indicator elements Sr and Ca, and analyzing the change of Sr/Ca values from the core to the edge of the sagittal plane of the otolith; (6) and evaluating the influence of the hydro-junction on the fish habitat. The method has the advantages of rapidness and high accuracy. A new method is provided for evaluating the influence of the hydro-hub construction on the aquatic habitat.
Description
Technical Field
The invention belongs to the field of aquatics, and relates to a method for evaluating influence of a hydro junction on a fish habitat based on otolith micro-area in-situ trace elements.
Background
The Guangdong bream is one of the important edible economic fishes in China, is a large river migratory fish, lives in the middle and lower layers of a water body, is omnivorous, produces viscous eggs, and is mainly distributed in the middle and lower reaches of the Zhujiang, the Wanquan river in the Hainan and part of the river of the continental river. The Guangdong bream is one of the main fishing objects in the middle and lower river sections of the Zhujiang river, and the proportion of the quantity of the fish-harvested objects can reach 44.1 percent. Historical data shows that the fishing amount of the Guangdong breams in the flood season of the domestic fish in the 20 th century and the fishing amount of the Guangdong breams in the 60 th century only reach 20t, and the resource amount of the Guangdong breams declines to about 5t in the 80 th century. However, how to evaluate the influence of the construction of the hydro-junction on the habitat of the Guangdong bream is a key problem which needs to be solved urgently at present.
During the development and growth of fish, chemical elements in the water environment are deposited in the otolith in the form of trace elements, and the trace elements deposited on the otolith are not decomposed or reabsorbed and are permanent. The microchemical composition from the central core region to the outer edge region of the otolith typically records, in a time-series of growths, the changes in the water chemistry environment experienced by individual fish from birth to capture.
Disclosure of Invention
The invention provides a method for evaluating influence of a hydro hub on a fish habitat based on in-situ trace elements in a micro-area of an otolith, and aims to analyze the change of the habitat of Guangdong bream based on the trace elements deposited on the otolith of the Guangdong bream and further evaluate the influence of the construction of the hydro hub on the habitat of the Guangdong bream
The invention is realized by the following technical scheme:
a method for evaluating the influence of a hydro-junction on a fish habitat based on otolith micro-area in-situ trace elements comprises the following steps:
(1) collecting samples: collecting Guangdong bream samples along the line of the hydraulic junction;
(2) extracting otolith: extracting otolith from a balance sac at the head of a Guangdong bream sample and grinding the otolith;
(3) making otolith slices: putting the otolith into a grinding mold, adding the prepared cold buried resin for embedding, and placing the otolith in a shade place for hardening; the function is as follows: the otolith sample is small and is fixed in the grinding tool by resin, thereby facilitating grinding
(4) The otolith in-situ micro-area trace element analysis method comprises the following steps: using LA-ICP-MS laser ablation sampling, and adopting a line scanning mode;
(5) screening out the indicator elements Sr and Ca, and analyzing the change of Sr/Ca values from the core to the edge of the sagittal plane of the otolith;
(6) and evaluating the influence of the hydro-junction on the fish habitat.
As a preferred embodiment of the present invention: in the step (3), in the grinding process, the waterproof wear-resistant abrasive paper of 120grit, 600grit, 1200grit, 2000grit and 2500grit is sequentially selected and used on a grinding die to be ground to the core along the longitudinal section of the otolith, different abrasive paper particle sizes are used, scratches of a grinding plane are reduced, the whole process needs to be observed under a microscope, after one surface is ground, the process is repeated to finish grinding of the other surface of the otolith, after two surfaces of the otolith are ground to the core, 0.3 mu m of alumina water-soluble cloth is used for polishing, the preparation of the otolith slice is finished, alumina polishing is used, scratches left in the grinding process are removed, and the interference of the observation of growth lines of the otolith is reduced.
As a preferred embodiment of the present invention: in the step (4), the diameter of the denudation beam is 40 μm, the frequency is 10Hz, the energy density is about 7.99J/cm2, the linear scanning speed is 5um/s, high-purity helium gas is used as a carrier gas, and the scheme is suitable for otolith samples through tests to extract the trace elements on the otolith samples.
As a preferred embodiment of the present invention: in the step (4), the NIST 610 is used for debugging the instrument before the test to enable the instrument to reach the optimal state, 20s of blank is received before and after the sample test, and the sample test time is determined according to the test length.
As a preferred embodiment of the present invention: in the step (4), a group of NIST 610, NIST 612 and MACS-3 is inserted into each 3-4 lines of ablation to calculate the element content, the off-line processing of the analysis data is completed by adopting software ICPMSDataCal, and the data correction is carried out by taking the NIST 612 as an external standard.
As a preferred embodiment of the present invention: in the step (4), the off-line processing of the analysis data comprises the selection of a sample and a blank signal, the correction of the sensitivity drift of the instrument and the calculation of element content.
As a preferred embodiment of the present invention: in the step (3), the otolith is selected from a right otolith.
As a preferred embodiment of the present invention: in the step (3), the otolith is selected from a left otolith.
Has the advantages that: the method analyzes the habitat change of the Guangdong bream based on the trace elements deposited on the Guangdong bream otolith, and further evaluates the influence of the construction of the hydro junction on the habitat of the Guangdong bream. The method has the advantages of rapidness and high accuracy. By comparing the difference of the in-situ trace elements of the otolith micro-area between Guangdong bream groups on the Changthou hydro junction dam and under the dam, the trace element change recorded from the core (0) to the edge of the sagittal plane of the otolith is analyzed, the change of the Sr/Ca value of a key element is screened out, and then the influence of the hydro junction construction on the habitat of the Guangdong bream is evaluated. A new method is provided for evaluating the influence of the hydro-hub construction on the aquatic habitat.
Drawings
FIG. 1 is a graph showing the Sr/Ca value variation recorded by analysis of the quantitative line from the core (0) to the edge of the sagittal plane of the megalobrama amblycephala on the Changzhou dam.
FIG. 2 is a graph showing the Sr/Ca value variation recorded by analysis of the quantitative line from the core (0) to the edge of the sagittal plane of the megalobrama amblycephala under the Changtui dam.
Detailed Description
The embodiments of the present invention will be described in detail below with reference to the accompanying drawings: the present embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a specific operation process are given, but the protection scope of the present invention is not limited to the following embodiments.
Example (b):
a method for evaluating the influence of a hydro-junction on a fish habitat based on otolith micro-area in-situ trace elements comprises the following steps:
(1) collecting samples: along the line of the hydro junction, the Guangdong bream sample is collected.
(2) Extracting otolith: otoliths were extracted from the balancing sac of the head of the megalobrama amblycephala sample and ground.
(3) Making otolith slices: and (3) putting the dextrogyra stone into a grinding mold, adding the prepared cold buried resin for embedding, and placing the mixture in a shade place for hardening. In the step (3), in the grinding process, 120grit, 600grit, 1200grit, 2000grit and 2500grit waterproof wear-resistant abrasive paper is selected and used on a grinding die in sequence to grind to the core along the longitudinal section of the otolith, the whole process needs to be observed under a microscope, after one surface is ground, the process is repeated to finish grinding of the other surface of the otolith, and after both surfaces of the otolith are ground to the core, 0.3 mu m of alumina water-soluble cloth is used for polishing to finish the preparation of the otolith slice.
(4) The otolith in-situ micro-area trace element analysis method comprises the following steps: and (4) performing laser ablation sampling by using LA-ICP-MS in a line scanning mode. In the step (4), the diameter of the denudation beam is 40 μm, the frequency is 10Hz, the energy density is about 7.99J/cm2, the linear scanning speed is 5um/s, and high-purity helium gas is used as a carrier gas. In the step (4), the NIST 610 is used for debugging the instrument before the test to enable the instrument to reach the optimal state, 20s of blank is received before and after the sample test, and the sample test time is determined according to the test length. In the step (4), a group of NIST 610, NIST 612 and MACS-3 is inserted into each 3-4 lines of ablation to calculate the element content, the off-line processing of the analysis data is completed by adopting software ICPMSDataCal, and the data correction is carried out by taking the NIST 612 as an external standard. In the step (4), the off-line processing of the analysis data comprises the selection of a sample and a blank signal, the correction of the sensitivity drift of the instrument and the calculation of element content.
(5) And screening out the indicator elements Sr and Ca, and analyzing the change of Sr/Ca values from the core to the edge of the sagittal plane of the otolith.
(6) And evaluating the influence of the hydro-junction on the fish habitat.
As shown in fig. 1 and 2, the abscissa represents the position from the core of the otolith, i.e., the life history of the guangdong bream, and 0 represents the core, i.e., the birth. The ordinate represents the Sr/Ca change deposited in otoliths in the life process of the Guangdong bream and is related to the change of the living water environment. FIG. 1 shows the Sr/Ca value fluctuation rule of Guangdong bream otolith at the upstream of Changzhou hydro junction, the Sr/Ca value fluctuation range is 0.92-1.69, the Sr/Ca value of the whole otolith is lower and is below 3, and the whole life history is shown in fresh water. FIG. 2 shows that the Sr/Ca value of Guangdong bream otolith at the downstream of Changzhou hydro junction fluctuates regularly around 3, which shows that the developmental stage shows the life history of migration between fresh water and brackish water river mouths.
The hydraulic junction has obvious influence on the habitat of the Guangdong bream. The ecological environment of Guangdong bream in the upper and lower river sections of the Changzhou dam has very obvious difference. The life history of the megalobrama amblycephala at the upstream of the dam belongs to type I, which shows that the whole process is finished in a freshwater habitat and does not enter brackish water at a river mouth. The megalobrama amblycephala population downstream of the dam shows two life history types II and III, wherein the type II (comprising S1, S2, S3 and S5) live in freshwater in the prehistoric period of life, then swim to a river mouth with higher salinity for fattening, and swim to a freshwater spawning ground for breeding after sexual maturity, and show periodic reproductive migration behavior. Type III (S4) migrates to fresh water spawning sites for reproduction at estuaries with higher salinity in early stages of life history and after sexual maturity, and exhibits periodic reproductive migration behavior.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A method for evaluating the influence of a hydro-junction on a fish habitat based on otolith micro-area in-situ trace elements is characterized by comprising the following steps:
(1) collecting samples: collecting Guangdong bream samples along the line of the hydraulic junction;
(2) extracting otolith: extracting otolith from a balance sac at the head of a Guangdong bream sample and grinding the otolith;
(3) making otolith slices: putting the otolith into a grinding mold, adding the prepared cold buried resin for embedding, and placing the otolith in a shade place for hardening;
(4) the otolith in-situ micro-area trace element analysis method comprises the following steps: using LA-ICP-MS laser ablation sampling, and adopting a line scanning mode;
(5) screening out the indicator elements Sr and Ca, and analyzing the change of Sr/Ca values from the core to the edge of the sagittal plane of the otolith;
(6) and evaluating the influence of the hydro-junction on the fish habitat.
2. The method for evaluating the influence of the hydro-junction on the habitat of the fish as claimed in claim 1, wherein in the step (3), in the grinding process, 120grit, 600grit, 1200grit, 2000grit and 2500grit waterproof wear-resistant abrasive paper is selected and used on a grinding mold to grind to the core along the longitudinal section of the otolith, the whole process is observed under a microscope, after one side grinding is completed, the above processes are repeated to complete the grinding of the other side of the otolith, and after both sides of the otolith are ground to the core, 0.3 μm alumina water-soluble cloth is used for polishing to complete the preparation of the otolith slice.
3. The method for evaluating the influence of the hydro-junction on the habitat of fish according to claim 1, wherein in the step (4), the ablation beam has a diameter of 40 μm, a frequency of 10Hz, an energy density of about 7.99J/cm2, a linear sweeping speed of 5 μm/s, and high-purity helium gas is used as a carrier gas.
4. The method for evaluating the influence of the hydro-junction on the habitat of the fish according to the otolith micro-area in-situ trace elements as claimed in claim 1, wherein in the step (4), a NIST 610 is used for debugging an instrument to reach an optimal state before testing, 20s of blank is received before and after sample testing, and the sample testing time is determined according to the testing length.
5. The method for evaluating the influence of the hydro-junction on the habitat of the fish on the basis of the otolith micro-area in-situ trace elements as claimed in claim 1, wherein in the step (4), a group of NIST 610, NIST 612 and MACS-3 is inserted into each 3-4 lines of denudation to calculate the content of the elements, the offline processing of the analyzed data is completed by software ICPMSDataCal, and the NIST 612 is used as an external standard for data correction.
6. The method for evaluating the influence of the hydro-junction on the fish habitat based on the otolith micro-area in-situ trace elements as claimed in claim 1, wherein in the step (4), the off-line processing of the analysis data comprises selection of sample and blank signals, instrument sensitivity drift correction and element content calculation.
7. The method for evaluating the influence of the hydro-junction on the habitat of fish on the basis of the micro-area in-situ trace elements of the otolith as claimed in claim 1, wherein in the step (3), the otolith is selected from the group consisting of the auricle.
8. The method for evaluating the influence of the hydro-junction on the habitat of fish according to the micro-area in-situ trace elements of otolith, as claimed in claim 1, wherein in the step (3), the otolith is selected from laevo otolith.
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Cited By (2)
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| CN114486442A (en) * | 2022-01-28 | 2022-05-13 | 公安部物证鉴定中心 | Sample preparation method for trace material evidence element analysis |
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Cited By (3)
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| CN113588328B (en) * | 2021-08-10 | 2022-04-19 | 中国水产科学研究院黑龙江水产研究所 | Micro-sampling method for micro-chemical analysis of otolith |
| CN114486442A (en) * | 2022-01-28 | 2022-05-13 | 公安部物证鉴定中心 | Sample preparation method for trace material evidence element analysis |
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