CN113176217A - Carrot-based chemical and material analysis professional course experimental method - Google Patents
Carrot-based chemical and material analysis professional course experimental method Download PDFInfo
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- CN113176217A CN113176217A CN202110330347.2A CN202110330347A CN113176217A CN 113176217 A CN113176217 A CN 113176217A CN 202110330347 A CN202110330347 A CN 202110330347A CN 113176217 A CN113176217 A CN 113176217A
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- 244000000626 Daucus carota Species 0.000 title claims abstract description 85
- 235000002767 Daucus carota Nutrition 0.000 title claims abstract description 85
- 238000002474 experimental method Methods 0.000 title claims abstract description 26
- 238000004458 analytical method Methods 0.000 title claims abstract description 23
- 239000000463 material Substances 0.000 title claims abstract description 22
- 239000000126 substance Substances 0.000 title claims abstract description 18
- 235000015190 carrot juice Nutrition 0.000 claims abstract description 74
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 68
- 239000003208 petroleum Substances 0.000 claims abstract description 34
- 239000002048 multi walled nanotube Substances 0.000 claims abstract description 33
- 238000010438 heat treatment Methods 0.000 claims abstract description 21
- 238000003756 stirring Methods 0.000 claims abstract description 20
- 238000012360 testing method Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 11
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims description 9
- 238000004506 ultrasonic cleaning Methods 0.000 claims description 8
- 238000002835 absorbance Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 description 12
- 150000001746 carotenes Chemical class 0.000 description 8
- 235000005473 carotenes Nutrition 0.000 description 8
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 7
- 239000002994 raw material Substances 0.000 description 7
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 7
- 238000010521 absorption reaction Methods 0.000 description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 6
- 238000000605 extraction Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 238000000862 absorption spectrum Methods 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 2
- 150000001336 alkenes Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/24—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for chemistry
Abstract
The invention provides a carrot-based experiment method for a professional chemical and material analysis course, which is implemented according to the following steps: step 1, squeezing carrot to obtain carrot juice and carrot paste; step 2, separating the carrot juice obtained in the step 1 from the carrot juice; step 3, extracting the carrot juice separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration‑3g/L‑10‑2Heating and uniformly stirring the multi-wall carbon nano tube solution in g/L, wherein the volume ratio of the carrot juice to the petroleum ether to the carrot juice is 15-20: 10-15: 5-6; step 4, extracting the carrot mud separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration into the carrot mud‑3g/L‑10‑ 2Heating and stirring uniformly after g/L of multi-wall carbon nano tube solution, wherein the volume of the added carrot juice into the petroleum ether is 1ml-1.5ml, and the volume of the added multi-wall carbon nano tube solution is 0.2ml-0.25 ml; and 5, respectively testing the products obtained in the steps 3 and 4 by using a double-beam ultraviolet-visible spectrophotometer.
Description
Technical Field
The invention belongs to the technical field of chemical and material analysis methods, and particularly relates to a carrot-based professional course experiment method for chemical and material analysis.
Background
In experimental teaching of courses such as analytical chemistry, material chemistry analysis, instrument analysis and the like, an ultraviolet-visible spectrophotometer is often used for measuring an absorption peak of a compound and testing the absorbance of the compound, so that the explanation and explanation of a chemical structure and a functional group of the compound are realized.
The traditional experimental method is usually carried out by adopting an extraction method, an extractant and a diluent are required to be added in the method, the environmental pollution is easily caused in the experimental process, heating and retainment cooling are required, and the required experimental equipment is relatively complex.
In order to better cope with the development trend of the world large industry and new industry, the fusion advantage between the industrial and scientific professions is exerted. The teaching concept of the university department is integrated in course experiment teaching, and the experiment teaching research of a professional course under the background of the university department needs to be started from the reality so as to improve the learning interest of students, the capability of solving actual problems, the practical capability and the comprehensive capability of analyzing problems.
Disclosure of Invention
The invention aims to provide a carrot-based experiment method for a professional chemical and material analysis course, which is characterized in that common carrots are used as raw materials, carotene is extracted, an ultraviolet-visible spectrophotometer is used for measuring an absorption peak of the extracted carotene and testing the absorbance of the carotene, so that the explanation and explanation of the chemical structure and functional groups of the carotene are realized, and the purposes of improving the learning interest of students, solving the actual problem, enabling the students to practice and analyzing the comprehensive capability of the problems are achieved.
In order to achieve the purpose, the invention adopts the technical scheme that: a chemical and material analysis professional course experiment method based on carrots is implemented according to the following steps:
step 2, separating the carrot juice obtained in the step 1 from the carrot juice;
step 3, extracting the carrot juice separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration-3g/L-10-2Heating and uniformly stirring the multi-wall carbon nano tube solution in g/L, wherein the volume ratio of the carrot juice to the petroleum ether to the carrot juice is 15-20: 10-15: 5-6;
step 4, extracting the carrot mud separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration into the carrot mud-3g/L-10-2Heating and stirring uniformly after g/L of multi-wall carbon nano tube solution, wherein the volume of the added carrot juice into the petroleum ether is 1ml-1.5ml, and the volume of the added multi-wall carbon nano tube solution is 0.2ml-0.25 ml;
and 5, respectively testing the products obtained in the steps 3 and 4 by using a double-beam ultraviolet-visible spectrophotometer.
As a preferred technical solution of the present invention, in the step 1, carrots are cut into blocks, and then put into a juicer to be juiced, so as to obtain carrot juice and carrot paste.
As a preferred technical scheme of the present invention, in the step 2, the carrot juice and carrot mud obtained in the step 1 are firstly filtered by micro-nano filter paper, the obtained liquid is vibrated in an ultrasonic cleaning machine and then filtered by the micro-nano filter paper again, so as to obtain the carrot juice, and the product isolated by the micro-nano filter paper is carrot mud.
As a preferable technical scheme of the invention, in the step 3 and the step 4, the heating and stirring temperature is 20-24 ℃, and the time is 8-10 min.
In the step 5, the test wavelength selected by the dual-beam uv-vis spectrophotometer is 200nm to 800nm, the test parameter is set to absorbance, and the test step is 0.05nm to 0.1 nm.
The invention has the beneficial effects that: the invention discloses a carrot-based experiment method for chemistry and material analysis professional courses, which relates to multidisciplinary and student-based autonomous teaching experiments, and provides a method for adding trace multi-walled carbon nanotubes, so that the solubility of carotene in an organic solvent is increased, and an extraction method is not needed; compared with an extraction method, the experimental method is simple in experimental operation, does not need complex chemical extraction equipment, is high in interestingness, improves the autonomous learning ability of students, and also improves the learning interest of the students, the capability of solving practical problems, the practical capability and the comprehensive capability of analyzing problems.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a UV-VIS spectrum of carrot juice in an experimental method of a carrot-based chemistry and material analysis professional course according to the present invention;
FIG. 2 is a UV-VIS spectrum of mashed carrot in an experimental method based on a carrot chemistry, material analysis professional course.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
The invention relates to a carrot-based chemical and material analysis professional course experimental method, which is implemented according to the following steps:
step 2, filtering the carrot juice and the carrot paste obtained in the step 1 by using micro-nano filter paper, oscillating the obtained liquid in an ultrasonic cleaning machine, and filtering by using the micro-nano filter paper again to obtain the carrot juice, wherein the product isolated by the micro-nano filter paper is the carrot paste;
step 3, extracting the carrot juice separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration-3g/L-10-2Heating and stirring the multi-walled carbon nanotube solution at the temperature of between 20 and 24 ℃ for 8 to 10 minutes, wherein the volume ratio of the carrot juice to the petroleum ether to the carrot juice is 15 to 20: 10-15: 5-6;
step 4, extracting the carrot mud separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration into the carrot mud-3g/L-10-2Heating and stirring the multi-walled carbon nanotube solution of g/L at the temperature of between 20 and 24 ℃ for 8 to 10 minutes, wherein the volume of the added carrot juice into the petroleum ether is 1 to 1.5ml, and the volume of the added multi-walled carbon nanotube solution is 0.2 to 0.25 ml;
and 5, respectively testing the products obtained in the steps 3 and 4 by using a double-beam ultraviolet-visible spectrophotometer.
The ultraviolet absorption spectra of the carotenes obtained from the carrot puree and the carrot juice in the experimental method of the invention are used respectively for qualitative analysis. The method can be used for obtaining obvious absorption peaks no matter the carrot paste and the carrot juice, and shows that the method does not influence the measurement of the ultraviolet absorption peaks on the form of an experimental sample, so that the form of the experimental sample can be not strictly limited, the experimental sample can be in a liquid state or a liquid-solid state, and the experimental success rate is high. Because carotene belongs to unsaturated conjugated olefin, the ultraviolet absorption spectrum has obvious red shift phenomenon. And calibrating the ultraviolet absorption peak and the absorbance value of the carotene through the identification of the spectrum peak. Comparing fig. 1 and fig. 2, the absorption peaks generated by the two substances are found to be identical, and the absorbance intensities are similar.
Example 1
The invention relates to a carrot-based chemical and material analysis professional course experimental method, which is implemented according to the following steps:
step 2, filtering the carrot juice and the carrot paste obtained in the step 1 by using micro-nano filter paper, oscillating the obtained liquid in an ultrasonic cleaning machine, and filtering by using the micro-nano filter paper again to obtain the carrot juice, wherein the product isolated by the micro-nano filter paper is the carrot paste;
step 3, extracting the carrot juice separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration-3Heating and stirring the multi-walled carbon nanotube solution at the temperature of 20 ℃ for 8min after the multi-walled carbon nanotube solution is in g/L, wherein the volume ratio of the carrot juice to the petroleum ether to the carrot juice is 15: 10: 5;
step 4, extracting the carrot mud separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration into the carrot mud-3Heating and stirring at 20 deg.C for 8min after adding multiwalled carbon nanotube solution of g/L, wherein the volume of the solution added with carrot juice is 1ml and the volume of the solution added with multiwalled carbon nanotube is 0.2 ml;
and 5, respectively testing the products obtained in the steps 3 and 4 by using a double-beam ultraviolet-visible spectrophotometer.
Example 2
The invention relates to a carrot-based chemical and material analysis professional course experimental method, which is implemented according to the following steps:
step 2, filtering the carrot juice and the carrot paste obtained in the step 1 by using micro-nano filter paper, oscillating the obtained liquid in an ultrasonic cleaning machine, and filtering by using the micro-nano filter paper again to obtain the carrot juice, wherein the product isolated by the micro-nano filter paper is the carrot paste;
step 3, extracting the carrot juice separated in the step 2, and sequentially adding petroleum ether and the carrot juice with the concentration of 5 multiplied by 10-3Heating and stirring the multi-walled carbon nanotube solution at the temperature of 22 ℃ for 9min after the multi-walled carbon nanotube solution is in g/L, wherein the volume ratio of the carrot juice to the petroleum ether to the carrot juice is 15: 12: 6;
step 4, extracting the carrot mud separated in the step 2, and sequentially adding petroleum ether and the mixture with the concentration of 5 multiplied by 10-3Heating and stirring at 22 deg.C for 9min after g/L of multi-walled carbon nanotube solution, wherein the volume of the added carrot juice is 1.2ml in petroleum ether and the volume of the added multi-walled carbon nanotube solution is 0.22ml in 1g carrot juice;
and 5, respectively testing the products obtained in the steps 3 and 4 by using a double-beam ultraviolet-visible spectrophotometer.
Example 3
The invention relates to a carrot-based chemical and material analysis professional course experimental method, which is implemented according to the following steps:
step 2, filtering the carrot juice and the carrot paste obtained in the step 1 by using micro-nano filter paper, oscillating the obtained liquid in an ultrasonic cleaning machine, and filtering by using the micro-nano filter paper again to obtain the carrot juice, wherein the product isolated by the micro-nano filter paper is the carrot paste;
step 3, extracting the carrot juice separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration-2Heating and stirring the multi-walled carbon nanotube solution at 24 ℃ for 10min after the multi-walled carbon nanotube solution is in g/L, wherein the volume ratio of the carrot juice to the petroleum ether to the carrot juice is 20: 10: 5;
step 4, extracting the carrot mud separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration into the carrot mud-2Heating and stirring at 24 deg.C for 10min after adding multiwalled carbon nanotube solution of g/L, wherein the volume of the solution added with carrot juice is 1.5ml and the volume of the solution added with multiwalled carbon nanotube is 0.25 ml;
and 5, respectively testing the products obtained in the steps 3 and 4 by using a double-beam ultraviolet-visible spectrophotometer.
Example 4
The invention relates to a carrot-based chemical and material analysis professional course experimental method, which is implemented according to the following steps:
step 2, filtering the carrot juice and the carrot paste obtained in the step 1 by using micro-nano filter paper, oscillating the obtained liquid in an ultrasonic cleaning machine, and filtering by using the micro-nano filter paper again to obtain the carrot juice, wherein the product isolated by the micro-nano filter paper is the carrot paste;
step 3, extracting the carrot juice separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration-2Heating and stirring the multi-walled carbon nanotube solution at 24 ℃ for 8-10 min after the multi-walled carbon nanotube solution is in g/L, wherein the volume ratio of the carrot juice to the petroleum ether to the carrot juice is 15: 15: 6;
step 4, extracting the carrot mud separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration into the carrot mud-2Heating and stirring at 24 deg.C for 9min after g/L of multi-walled carbon nanotube solution, wherein the volume of the solution added into 1g carrot juice and petroleum ether is 1.5ml, and the volume of the solution added into multi-walled carbon nanotube solution is 0.2 ml;
and 5, respectively testing the products obtained in the steps 3 and 4 by using a double-beam ultraviolet-visible spectrophotometer.
Example 5
The invention relates to a carrot-based chemical and material analysis professional course experimental method, which is implemented according to the following steps:
step 2, filtering the carrot juice and the carrot paste obtained in the step 1 by using micro-nano filter paper, oscillating the obtained liquid in an ultrasonic cleaning machine, and filtering by using the micro-nano filter paper again to obtain the carrot juice, wherein the product isolated by the micro-nano filter paper is the carrot paste;
step 3, extracting the carrot juice separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration-3Heating and stirring the multi-walled carbon nanotube solution at the temperature of 20 ℃ for 8min after the multi-walled carbon nanotube solution is in g/L, wherein the volume ratio of the carrot juice to the petroleum ether to the carrot juice is 20: 15: 6;
step 4, extracting the carrot mud separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration into the carrot mud-3Heating and stirring at 22 deg.C for 10min after adding multiwall carbon nanotube solution of g/L, wherein volume of 1.5ml of carrot juice added with petroleum ether and multiwall carbon nanotube addedThe volume of the solution was 0.25 ml;
and 5, respectively testing the products obtained in the steps 3 and 4 by using a double-beam ultraviolet-visible spectrophotometer.
While the foregoing description shows and describes several preferred embodiments of the invention, it is to be understood, as noted above, that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (5)
1. A chemical and material analysis professional course experiment method based on carrots is characterized by comprising the following steps:
step 1, squeezing carrot to obtain carrot juice and carrot paste;
step 2, separating the carrot juice obtained in the step 1 from the carrot juice;
step 3, extracting the carrot juice separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration-3g/L-10-2Heating and uniformly stirring the multi-wall carbon nano tube solution in g/L, wherein the volume ratio of the carrot juice to the petroleum ether to the carrot juice is 15-20: 10-15: 5-6;
step 4, extracting the carrot mud separated in the step 2, and sequentially adding petroleum ether and 10-degree concentration into the carrot mud-3g/L-10-2Heating and stirring uniformly after g/L of multi-wall carbon nano tube solution, wherein the volume of the added carrot juice into the petroleum ether is 1ml-1.5ml, and the volume of the added multi-wall carbon nano tube solution is 0.2ml-0.25 ml;
and 5, respectively testing the products obtained in the steps 3 and 4 by using a double-beam ultraviolet-visible spectrophotometer.
2. The carrot-based chemistry, material analysis professional lesson experimental method as claimed in claim 1, wherein in the step 1, carrot is cut into blocks and then put into a juicer to be juiced, so as to obtain carrot juice and carrot puree.
3. The carrot-based chemistry and material analysis professional course experimental method as claimed in claim 2, wherein in the step 2, the carrot juice and carrot mud obtained in the step 1 are firstly filtered by micro-nano filter paper, the obtained liquid is vibrated in an ultrasonic cleaning machine and then filtered by the micro-nano filter paper again, the carrot juice is obtained, and the product isolated by the micro-nano filter paper is the carrot mud.
4. The carrot-based chemistry, material analysis professional lesson experimental method as claimed in claim 3, wherein in the step 3 and the step 4, the temperature of heating and stirring is 20-24 ℃ and the time is 8-10 min.
5. The carrot-based chemistry, material analysis professional lesson experimental method as claimed in claim 4, wherein in the step 5, the double beam UV-visible spectrophotometer selects the test wavelength to be 200nm-800nm, the test parameter is set to be absorbance, and the test step size is 0.05nm-0.1 nm.
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