CN113170788A

CN113170788A - Application of honokiol as agricultural bactericide

Info

Publication number: CN113170788A
Application number: CN202110454082.7A
Authority: CN
Inventors: 宋萍萍; 王雨; 韦敏; 钱怡云; 颜露
Original assignee: Institute of Botany of CAS
Current assignee: Institute of Botany of CAS
Priority date: 2021-04-26
Filing date: 2021-04-26
Publication date: 2021-07-27
Anticipated expiration: 2041-04-26
Also published as: CN113170788B

Abstract

The invention discloses an application of a compound honokiol as an agricultural bactericide. The compound is used as a bactericide for preventing and treating agricultural fungal diseases, and can effectively prevent and treat crop diseases such as sclerotinia rot, banded sclerotial blight, gray mold, gibberellic disease, anthracnose, bakanae disease, gummy stem blight, fusarium wilt, verticillium wilt, rice blast and the like. The agricultural bactericide prepared by taking honokiol as an active ingredient has the characteristics of good prevention and treatment effect, broad spectrum, low toxicity, no residue, no pollution and no public nuisance, and is particularly beneficial to environmental protection.

Description

Application of honokiol as agricultural bactericide

Technical Field

The invention belongs to the technical field of agriculture, and particularly relates to application of a compound honokiol as an active ingredient in preparation of an agricultural bactericide.

Background

Diseases of crops are not easy to be detected by people in time like pests and weeds, so that neglect and difficulty in prevention and control are caused, and the damage is more serious. It is reported that plant diseases caused by pathogenic fungi alone account for as many as ten thousand in the world, and the loss caused accounts for 10% -30% of the annual total loss of crops. If plant diseases caused by viruses, bacteria, nematodes and the like are counted, the loss is more remarkable.

In order to meet the increasing demand of people for food and other living materials, chemicals have to be applied to protect crops from harmful organisms, of which bactericides play an important role. Natural products with agricultural bactericidal activity are generally easy to be absorbed and conducted by plants, and have little or no phytotoxicity to plants, so that the natural products are paid much attention.

Compound Honokiol, the name of English is Honokiol, the CAS number is 35354-74-6, the molecular formula is C₁₈H₁₈O₂266.33, molecular weight:

the compound is yellowish brown to yellowish or white powder, and is dissolved in organic solvent such as methanol, ethanol, acetone, ethyl acetate, chloroform, etc. The research on the activity of the compound for inhibiting the phytopathogen is less.

Disclosure of Invention

The invention aims to explore the application of honokiol as an active ingredient in preparing agricultural fungicides.

A large number of researches prove that the agricultural bactericide prepared by using the compound as an active ingredient has excellent bactericidal activity, can be applied to control fungal diseases on various crops, and has wide bacteriostatic spectrum, such as: sclerotinia rot, banded sclerotial blight, gray mold, gibberellic disease, anthracnose, bakanae disease, gummy stem blight, fusarium wilt, verticillium wilt and rice blast.

The honokiol can be obtained by separating and purifying natural plants, or by chemical synthesis. The compound is used as an active ingredient, a pesticide auxiliary agent and an excipient to be prepared into any formulation allowed on pesticides. It can also be compounded with other pesticides, and mixed with pesticide adjuvant and excipient to make any dosage form allowable on pesticides. The preparation formulation can be missible oil, a suspending agent, an aqueous emulsion, wettable powder, a microemulsion, water dispersible granules and the like. The used auxiliary agent can adopt calcium dodecyl benzene sulfonate, pyrrolidone, polyoxyethylene polyoxypropylene block compound, nonylphenol polyoxyethylene ether and the like, and the excipient can be cyclohexanone, solvent oil, water, propylene glycol, deionized water and the like. Other pesticides used for compounding can be organic bactericides, such as triazole bactericides triadimenol; botanical bactericides such as allicin, osthole, etc.

Compared with the prior art, the invention has the beneficial effects that: the honokiol is used as the bactericide, has wide antibacterial spectrum and long lasting period, and is an ideal bactericide in agriculture. Compared with common chemical pesticides, the plant extract is derived from natural plants, has the characteristics of low toxicity, no residue, no pollution and no public nuisance for various economic crops such as grains, melons, fruits, vegetables and the like, and is more beneficial to environmental protection.

Detailed Description

The effects of the present invention are further illustrated by the following specific embodiments:

example 1: prevention and treatment of agricultural diseases by honokiol

Test strains Rhizoctonia solani (A)Thanatephorus cucumeris)Separating from rice sheath blight disease leaves; sclerotinia sclerotiorum (A) and (B)Sclerotioia selerotiorum) Separating from sclerotinia rot of colza strain; botrytis cinerea (A. cinerea)Botrytis cinerea) Separating from strawberry gray mold; fusarium graminearum (F.), (Fusarium graminearum) Separating from wheat scab; colletotrichum gloeosporioides (B)Colletotrichum gloeosporioides) Separating from grape anthracnose disease plants; fusarium moniliforme (F.), (Fusarium moniliforme) Is separated from the rice bakanae disease strain. The six strains are provided by the plant protection institute of Nanjing university of agriculture. Shell fungus of black rot melon (Didymella bryoniae) Magnaporthe grisea separated from gummy stem blight of watermelon (Magnaporthe oryzae) Is separated from rice blast disease plants, and the two strains are provided by plant research institute of China academy of sciences in Jiangsu province. Fusarium oxysporum (F.), (Fusarium oxysporum) Verticillium dahliae separated from cotton wilt disease plant (A), (B), (C)Verticillium dahliae) Is separated from cotton verticillium wilt disease plants and is provided by the plant protection of agricultural academy of Jiangsu province.

Dissolving test compound honokiol in DMSO to prepare 1000 μ g/mL mother liquor, and storing for use. PDA culture medium, 200g peeled potato is cut into blocks, boiled for 10min, juice is taken, 20g agar is added, heating is carried out until melting, 20g glucose is added, and water is added for constant volume until 1000 mL.

The inhibition of hypha growth is determined by hypha growth rate determination, a bacterial dish (diameter 5mm) is punched from the edge of a test bacterium pre-cultured on a PDA plate, the mother liquor of the test reagent is uniformly mixed with a culture medium, the final concentration of the test reagent is respectively 30, 20, 10, 5, 2.5 and 0ppm, the mixed liquor is respectively poured into a sterilized culture dish with the diameter of 9cm to prepare a plate, each dish is inoculated with a bacterial dish, the hypha face upwards, and each treatment is repeated for 3 times. And (4) culturing at 25 ℃ until the control colonies are more than two thirds of the full culture dish, and measuring the colony growing diameter by a cross method. The growth inhibition rate was calculated from the average colony diameter (mm). The obtained data is analyzed and processed by DPS V3.01 professional version software to obtain a standard curve and EC₅₀The value is obtained.

The results show that the compound honokiol can effectively prevent and treat sclerotinia sclerotiorum, banded sclerotial blight, gray mold, gibberellic disease, anthracnose, bakanae disease, gummy stem blight, fusarium wilt, verticillium wilt and rice blast, and inhibit the medium-concentration EC of the diseases₅₀The concentration is 4.3684-18.5477 mg/L, and the specific experimental results are shown in Table 1. The obvious effect of the pesticide is fully embodied through experiments.

TABLE 1 bacteriostatic rate of the compounds on agricultural diseases

Plant pathogenic bacteria	Regression equation	R	EC₅₀(mg/L)	95% confidence interval
					Rhizoctonia solani	y=4.5298+0.6516x	0.9972	5.2677	4.6782~5.9315
Sclerotinia sclerotiorum	y=0.2656+3.9774x	0.9619	15.4999	11.753~20.4412
					Fusarium graminearum	y=4.1838+1.0270x	0.9803	6.2331	4.6972~8.2711
Colletotrichum gloeosporioides	y=3.1194+1.4828x	0.9719	18.5477	14.9536~23.0057
					Botrytis cinerea	y=4.5867+0.3927x	0.9681	11.2807	8.7735~14.5045
Fusarium moniliforme	y=4.0465+1.3139x	0.9998	5.318	5.2018~5.4368
					Fusarium oxysporum	y=3.5219+1.4663x	0.9662	10.1877	7.7803~13.34
Pediococcus cucurbitae	y=3.9187+1.6887x	0.9989	4.3684	4.0218~4.7449
					Verticillium dahliae	y=1.9773+2.4231x	0.9891	17.6794	15.1654~20.6102
Magnaporthe grisea	y=2.5366+3.0771x	0.9124	6.3176	3.3288~11.9898

Example 2:

weighing the compound and conventional auxiliary materials such as lignin, diatomite and the like, and preparing the wettable powder with the effective component concentration of 2% according to a conventional process.

Example 3:

the compound is weighed and mixed with conventional auxiliary materials such as calcium dodecyl benzene sulfonate, phenethyl phenol polyoxyethylene ether, solvent oil and the like according to a conventional process to prepare missible oil with the concentration of the effective component of 2 percent.

Example 4:

the compound and conventional auxiliary materials such as sodium lignosulphonate, kaolin and the like are weighed and prepared into water dispersible granules with the effective component concentration of 2% according to a conventional process.

Example 5:

weighing the compound, solvent oil, pyrrolidone, xanthan gum and other conventional auxiliary materials, and preparing the mixture into an aqueous emulsion with the concentration of an effective component of 1% according to a conventional process.

Example 6:

weighing the compound and conventional auxiliary materials such as calcium dodecyl benzene sulfonate, pyrrolidone and the like, and preparing the microemulsion with the concentration of the effective component of 1% according to the conventional process.

Example 7:

according to the weight ratio of 1: 5, weighing the compound and the triadimenol, and preparing the compound and the triadimenol with conventional auxiliary materials such as solvent oil, pyrrolidone, xanthan gum and the like into the aqueous emulsion with the effective component concentration of 5 percent according to a conventional process.

Example 8:

according to the weight ratio of 6: 1, weighing the compound and the allicin, and preparing the compound and the allicin with conventional auxiliary materials such as solvent oil, pyrrolidone, xanthan gum and the like into an aqueous emulsion with the concentration of an effective component of 5 percent according to a conventional process.

Example 9:

according to the weight ratio of 5: 1, weighing the compound and osthole, and mixing with solvent oil, pyrrolidone, xanthan gum and other conventional auxiliary materials according to a conventional process to prepare the aqueous emulsion with the effective component concentration of 5%.

Claims

1. Use of honokiol as active ingredient in preparing agricultural bactericide.

2. The use according to claim 1, characterized in that the compound is used as an active ingredient and pesticide adjuvant and excipient to be prepared into any one of pesticide allowable dosage forms; or the compound is used as an active ingredient and is prepared into any one of pesticide allowable dosage forms together with other pesticides and pesticide auxiliary agents and excipients.

3. Use according to claim 2, characterized in that the formulation is an emulsifiable concentrate, a suspension, an aqueous emulsion, a wettable powder, a microemulsion or a water dispersible granule; the other pesticides are triazole fungicide triadimenol or botanical fungicides allicin and osthole.

4. The use according to claim 1, characterized in that the fungicide is used for controlling agricultural fungal diseases.

5. The use according to claim 4, characterized in that the agricultural fungal diseases are sclerotinia sclerotiorum, banded sclerotial blight, gray mold, head blight, anthracnose, bakanae disease, gummy stem blight, fusarium wilt, verticillium wilt, rice blast.