CN113166770A - 重组大肠杆菌***及其构建方法和其在合成α-1,2-岩藻糖基化寡糖中的应用 - Google Patents
重组大肠杆菌***及其构建方法和其在合成α-1,2-岩藻糖基化寡糖中的应用 Download PDFInfo
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Abstract
涉及基因工程技术领域,提供了重组大肠杆菌***及其构建方法和其在合成α‑1,2‑岩藻糖基化寡糖中的应用。该重组大肠杆菌***具有α‑1,2‑岩藻糖基转移酶基因和GDP‑岩藻糖焦磷酸化酶基因。通过将α‑1,2‑岩藻糖基转移酶基因和GDP‑岩藻糖焦磷酸化酶基因转化到大肠杆菌中,构建基因工程菌,以表达α‑1,2‑岩藻糖基转移酶和GDP‑岩藻糖焦磷酸化酶,并将表达的α‑1,2‑岩藻糖基转移酶和GDP‑岩藻糖焦磷酸化酶用于酶法合成α‑1,2‑岩藻糖基化寡糖的。
Description
PCT国内申请,说明书已公开。
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- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (3)
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CN2019111195059 | 2019-11-15 | ||
CN201911119505 | 2019-11-15 | ||
PCT/CN2019/120873 WO2021093022A1 (zh) | 2019-11-15 | 2019-11-26 | 重组大肠杆菌***及其构建方法和其在合成α-1,2-岩藻糖基化寡糖中的应用 |
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CN113166770A true CN113166770A (zh) | 2021-07-23 |
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CN (1) | CN113166770A (zh) |
AU (1) | AU2019400151B2 (zh) |
WO (1) | WO2021093022A1 (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115466707A (zh) * | 2022-06-27 | 2022-12-13 | 天津科技大学 | 一种生产2′-岩藻糖基乳糖的乳酸乳球菌及其应用 |
CN116286919A (zh) * | 2021-12-03 | 2023-06-23 | 虹摹生物科技(上海)有限公司 | 基因工程菌及利用其制备岩藻糖基化寡糖的方法 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113337554A (zh) * | 2021-05-28 | 2021-09-03 | 华东理工大学 | 一种体外多酶级联催化合成岩藻糖基化乳糖的方法 |
CN113789341B (zh) * | 2021-09-16 | 2022-10-11 | 陕西理工大学 | BmSPI38的同型串联多聚体及其构建方法和应用 |
Citations (3)
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KR101648352B1 (ko) * | 2015-11-09 | 2016-08-16 | 서울대학교산학협력단 | 푸코실락토오스 생산용 재조합 대장균 및 이를 이용한 푸코실락토오스의 생산방법 |
CN108611384A (zh) * | 2012-08-20 | 2018-10-02 | 中央研究院 | 寡糖的大规模酶合成的方法 |
CN109554385A (zh) * | 2018-07-24 | 2019-04-02 | 石家庄葛兰德生物科技有限公司 | 一种基因工程菌制备2-岩藻糖基乳糖的方法 |
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2019
- 2019-11-26 WO PCT/CN2019/120873 patent/WO2021093022A1/zh active Application Filing
- 2019-11-26 AU AU2019400151A patent/AU2019400151B2/en active Active
- 2019-11-26 CN CN201980006916.9A patent/CN113166770A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108611384A (zh) * | 2012-08-20 | 2018-10-02 | 中央研究院 | 寡糖的大规模酶合成的方法 |
KR101648352B1 (ko) * | 2015-11-09 | 2016-08-16 | 서울대학교산학협력단 | 푸코실락토오스 생산용 재조합 대장균 및 이를 이용한 푸코실락토오스의 생산방법 |
CN109554385A (zh) * | 2018-07-24 | 2019-04-02 | 石家庄葛兰德生物科技有限公司 | 一种基因工程菌制备2-岩藻糖基乳糖的方法 |
Non-Patent Citations (4)
Title |
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NCBI REFERENCE SEQUENCE: WP_010993080.1: "kinase [Bacteroides fragilis]", 《NCBI》 * |
OH,J.D. 等: "Helicobacter pylori HPAG1, complete genome", 《GENBANK》 * |
WEI WANG等: "Chemoenzymatic synthesis of GDP-L-fucose and the Lewis X glycan derivatives", 《PNAS》 * |
YOUNG-WOOK CHIN 等: "Metabolic Engineering of Escherichia coli to produce 2’-Fucosyllactose via Salvage Pathway of guanosine 5’-Diphosphate(GDP)-L-Fucose", 《BIOTECHNOLOGY AND BIOENGINEERING》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116286919A (zh) * | 2021-12-03 | 2023-06-23 | 虹摹生物科技(上海)有限公司 | 基因工程菌及利用其制备岩藻糖基化寡糖的方法 |
CN115466707A (zh) * | 2022-06-27 | 2022-12-13 | 天津科技大学 | 一种生产2′-岩藻糖基乳糖的乳酸乳球菌及其应用 |
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WO2021093022A1 (zh) | 2021-05-20 |
AU2019400151B2 (en) | 2022-06-23 |
AU2019400151A1 (en) | 2021-06-03 |
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