CN113143548B - Artificial intervertebral disc tissue, construction method, preparation method, computer readable storage medium and equipment - Google Patents
Artificial intervertebral disc tissue, construction method, preparation method, computer readable storage medium and equipment Download PDFInfo
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- CN113143548B CN113143548B CN202110301861.3A CN202110301861A CN113143548B CN 113143548 B CN113143548 B CN 113143548B CN 202110301861 A CN202110301861 A CN 202110301861A CN 113143548 B CN113143548 B CN 113143548B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
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- A61F2/44—Joints for the spine, e.g. vertebrae, spinal discs
- A61F2/442—Intervertebral or spinal discs, e.g. resilient
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Abstract
The invention provides an artificial intervertebral disc tissue, a construction method, a preparation method, a computer readable storage medium and equipment. The artificial intervertebral disc tissue sequentially comprises a first cartilage plate, an intervertebral disc body and a second cartilage plate from top to bottom, and further comprises a nucleus pulposus unit, wherein the intervertebral disc body is provided with a nucleus pulposus cavity for accommodating the nucleus pulposus unit. The application of the invention can effectively replace degenerated intervertebral disc tissues, promote the activity of intervertebral discs, delay the aging of lumbar vertebrae and prolong the service life of intervertebral discs; simultaneously, the problems related to lumbar intervertebral disc excision, intervertebral fusion, caused intervertebral disc function deficiency, reduced activity of the spinal motion unit and replacement of the intervertebral disc metal prosthesis are reduced.
Description
Technical Field
The invention belongs to the technical field of intervertebral discs, and particularly relates to artificial intervertebral disc tissues, a construction method, a preparation method, a computer readable storage medium and equipment.
Background
The healthy intervertebral disc can absorb and disperse impact during the movement of vertebrae, which is beneficial to the movement of the spine. Most students consider that the initiating factor of lumbar retrogressive change is intervertebral disc, and as the age increases, the intervertebral disc gradually degenerates, and then the conditions of hyperosteogeny, osteophyte, ligament hypertrophy, calcification and the like appear, and the degeneration of the intervertebral disc is the initiating factor of subsequent structural degeneration, and the intervertebral disc itself cannot be regenerated.
At present, for the aged intervertebral disc in lumbar degenerative diseases, intervertebral disc cleaning and intervertebral bone grafting fusion operation are mostly adopted, namely, most of the intervertebral disc is excised, bones are stuffed, and the bones of the upper vertebral body and the lower vertebral body are grown together. And secondly, an artificial intervertebral disc replacement mode can be adopted. The former mode loses the mobility of this backbone unit, and the latter mode is more expensive, and artifical intervertebral disc is mostly the metal material simultaneously, and wear debris etc. can arouse foreign matter reaction.
Disclosure of Invention
In view of the above-described shortcomings of the prior art, it is an object of the present invention to provide artificial disc tissue and methods of construction, preparation, computer readable storage media, apparatus. The artificial intervertebral disc tissue sequentially comprises a first cartilage plate, an intervertebral disc body and a second cartilage plate from top to bottom, and further comprises a nucleus pulposus unit, wherein the intervertebral disc body is provided with a nucleus pulposus cavity for accommodating the nucleus pulposus unit. The application of the invention can effectively replace degenerated intervertebral disc tissues, promote the activity of intervertebral discs, delay the aging of lumbar vertebrae and prolong the service life of intervertebral discs; simultaneously, the problems related to lumbar intervertebral disc excision, intervertebral fusion, caused intervertebral disc function deficiency, reduced activity of the spinal motion unit and replacement of the intervertebral disc metal prosthesis are reduced.
To achieve the above and other related objects, a first aspect of the present invention provides an artificial disc tissue comprising, in order from top to bottom, a first cartilage plate, a disc body and a second cartilage plate, the artificial disc tissue further comprising a nucleus pulposus unit, the disc body being provided with a nucleus pulposus cavity accommodating the nucleus pulposus unit.
The second aspect of the present invention provides the method for constructing an artificial intervertebral disc tissue, comprising the steps of:
1) Constructing a lumbar model;
2) Determining a excision range, simulating excision on the lumbar model to obtain an excised lumbar model, and taking excision parts as an initial artificial intervertebral disc tissue model;
3) And constructing a first cartilage plate model, an intervertebral disc body model, a second cartilage plate model and a nucleus pulposus unit model by taking the outer contour of the initial artificial intervertebral disc tissue model as a reference, wherein the constructed intervertebral disc body model is provided with a nucleus pulposus cavity for accommodating the nucleus pulposus unit model.
A third aspect of the present invention provides a method for preparing the above-described artificial disc tissue, comprising: and constructing and obtaining an artificial intervertebral disc tissue model according to the construction method of the artificial intervertebral disc tissue model, and preparing the artificial intervertebral disc tissue.
A fourth aspect of the present invention provides a computer-readable storage medium having stored thereon a computer program which, when executed by a processor, implements the steps of the above-described method of constructing an artificial disc tissue model, or the steps of the above-described method of preparing an artificial disc tissue.
A fifth aspect of the invention provides an apparatus comprising: the device comprises a processor and a memory, wherein the memory is used for storing a computer program, and the processor is used for executing the computer program stored in the memory, so that the device executes the steps of the method for constructing the artificial intervertebral disc tissue model or the steps of the method for preparing the artificial intervertebral disc tissue.
As described above, the present invention has at least one of the following advantages:
1) The invention prepares the artificial intervertebral disc tissue with bioactivity through a tissue engineering technology, promotes the regeneration of the intervertebral disc, prolongs the service life of the intervertebral disc, delays the degeneration of the intervertebral disc and related tissues, and solves the problem that the existing intervertebral disc tissue cannot be replaced by a bioactive prosthesis.
2) The application of the invention can effectively replace degenerated intervertebral disc tissues, promote the activity of intervertebral discs, delay the aging of lumbar vertebrae and prolong the service life of intervertebral discs; simultaneously, the problems related to lumbar intervertebral disc excision, intervertebral fusion, caused intervertebral disc function deficiency, reduced activity of the spinal motion unit and replacement of the intervertebral disc metal prosthesis are reduced.
3) The invention reduces the treatment cost of lumbar vertebra degeneration diseases and solves the problems of pain, movement disorder and the like caused by spinal degeneration.
Drawings
Fig. 1 is a schematic view showing the structure of an artificial disc tissue according to the present invention.
Fig. 2 is a schematic view showing the structure of a second cartilage plate in the artificial disc tissue according to the present invention.
Fig. 3 is a schematic view showing the structure of the disc body, the second cartilage plate and the nucleus pulposus unit in the artificial disc tissue according to the invention.
Fig. 4 is a schematic view of the artificial disc of fig. 1, shown in a cut-away configuration.
Fig. 5 is a schematic diagram showing the structure of a nucleus pulposus unit in an artificial intervertebral disc tissue according to the invention.
Reference numerals:
1. first cartilage plate
11. First cartilage lamina
111. First cartilage unit
112. First connecting unit
2. Intervertebral disc body
21. Nucleus pulposus cavity
22. Intervertebral disc body layer
221. Intervertebral disc body component
2211. First intervertebral disc body unit
2212. Second intervertebral disc body unit
3. Second cartilage plate
31. Second cartilage lamina
311. Second cartilage unit
312. Second connection unit
4. Nucleus pulposus unit
Detailed Description
Further advantages and effects of the present invention will become apparent to those skilled in the art from the disclosure of the present invention, which is described by the following specific examples.
Please refer to fig. 1 to 5. It should be understood that the structures, proportions, sizes, etc. shown in the drawings are for illustration purposes only and should not be construed as limiting the invention to the extent that it can be practiced, since modifications, changes in the proportions, or otherwise, used in the practice of the invention, are not intended to be critical to the essential characteristics of the invention, but are intended to fall within the spirit and scope of the invention. Also, the terms such as "upper," "lower," "left," "right," "middle," and "a" and the like recited in the present specification are merely for descriptive purposes and are not intended to limit the scope of the invention, but are intended to provide relative positional changes or modifications without materially altering the technical context in which the invention may be practiced.
An artificial disc tissue comprising, in order from top to bottom, as shown in fig. 1, a first cartilage plate 1, a disc body 2 and a second cartilage plate 3, the artificial disc tissue further comprising a nucleus pulposus unit 4, the disc body 2 being provided with a nucleus pulposus cavity 21 accommodating the nucleus pulposus unit 4.
The artificial disc tissue can replace the defective disc tissue of the patient and has the mobility of the spinal unit.
In a preferred embodiment, the artificial disc tissue is generally cylindrical in shape.
In a preferred embodiment, as shown in fig. 1, the first cartilage plate 1 comprises several layers of first cartilage plate layers 11. The first cartilage plate 1 simulates the cartilage layer at the junction of the vertebral bodies of an intervertebral disc.
In a preferred embodiment, as shown in fig. 1, the first cartilage plate layer 11 comprises a number of parallel first cartilage units 111.
In a preferred embodiment, the first cartilage units 111 of adjacent layers intersect at an angle α of 30 to 90 °.
In a preferred embodiment, the materials of adjacent first cartilage units in the same first cartilage plate layer are a polymer and a hydrogel mixed with chondrocytes and chondrocyte growth factors, respectively. The high molecular polymer provides the required mechanical strength, and the hydrogel mixed with the chondrocytes and the chondrocyte growth factors enables the artificial intervertebral disc tissue to have biological activity, promotes the regeneration of the intervertebral disc, prolongs the service life of the intervertebral disc and delays the degeneration of the intervertebral disc and related tissues.
In a preferred embodiment, the high molecular polymer is selected from at least one of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid.
In a preferred embodiment, the chondrocyte growth factor is selected from one or more of bone morphogenic protein-4/7 and combined transcriptional growth β.
In a preferred embodiment, the chondrocyte density is 5000 to 8000 cells/mm 3 。
In a preferred embodiment, the concentration of chondrocyte growth factor is 10 to 100000ng/uL.
In a preferred embodiment, the materials of adjacent first chondrocyte layers are a high molecular polymer and a hydrogel mixed with chondrocytes and chondrocyte growth factors, respectively. The high molecular polymer provides the required mechanical strength, and the hydrogel mixed with the chondrocytes and the chondrocyte growth factors enables the artificial intervertebral disc tissue to have biological activity, promotes the regeneration of the intervertebral disc, prolongs the service life of the intervertebral disc and delays the degeneration of the intervertebral disc and related tissues.
In a preferred embodiment, the high molecular polymer is selected from at least one of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid.
In a preferred embodiment, the chondrocyte growth factor is selected from one or more of bone morphogenic protein-4/7 and combined transcriptional growth β.
In a preferred embodiment, the chondrocyte density is 5000 to 8000 cells/mm 3 。
In a preferred embodiment, the concentration of chondrocyte growth factor is 10 to 100000ng/uL.
In a preferred embodiment, as shown in fig. 1, the first cartilage plate layer 11 further comprises a first connection unit 112, and the first connection unit 112 connects adjacent first cartilage units 111.
In a preferred embodiment, the material of the first connection unit 112 is a high molecular polymer.
In a preferred embodiment, the high molecular polymer is selected from at least one of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid.
In a preferred embodiment, as shown in fig. 2, the second cartilage plate 3 comprises several layers of second cartilage plate layers 31. The second cartilage plate simulates the cartilage layer at the junction of the vertebral bodies of the disc.
In a preferred embodiment, as shown in fig. 2, the second cartilage plate layer 31 comprises a plurality of parallel second cartilage units 311.
In a preferred embodiment, the second chondrocyte 311 of an adjacent layer crosses an angle β of 30 to 90 °.
In a preferred embodiment, the material of adjacent second cartilage units in the same second cartilage plate layer is a polymer and a hydrogel mixed with chondrocytes and chondrocyte growth factors, respectively. The high molecular polymer provides the required mechanical strength, and the hydrogel mixed with the chondrocytes and the chondrocyte growth factors enables the artificial intervertebral disc tissue to have biological activity, promotes the regeneration of the intervertebral disc, prolongs the service life of the intervertebral disc and delays the degeneration of the intervertebral disc and related tissues.
In a preferred embodiment, the high molecular polymer is selected from at least one of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid.
In a preferred embodiment, the chondrocyte growth factor is selected from one or more of bone morphogenic protein-4/7 and combined transcriptional growth β.
In a preferred embodiment, the chondrocyte density is 5000 to 8000 cells/mm 3 。
In a preferred embodiment, the concentration of chondrocyte growth factor is 10 to 100000ng/uL.
In a preferred embodiment, the material of the adjacent second chondrocyte layer is a high molecular polymer and a hydrogel mixed with chondrocytes and chondrocyte growth factors, respectively. The high molecular polymer provides the required mechanical strength, and the hydrogel mixed with the chondrocytes and the chondrocyte growth factors enables the artificial intervertebral disc tissue to have biological activity, promotes the regeneration of the intervertebral disc, prolongs the service life of the intervertebral disc and delays the degeneration of the intervertebral disc and related tissues.
In a preferred embodiment, the high molecular polymer is selected from at least one of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid.
In a preferred embodiment, the chondrocyte growth factor is selected from one or more of bone morphogenic protein-4/7 and combined transcriptional growth β.
In a preferred embodiment, the chondrocyte density is 5000 to 8000 cells/mm 3 。
In a preferred embodiment, the concentration of chondrocyte growth factor is 10 to 100000ng/uL.
In a preferred embodiment, as shown in fig. 3, the second cartilage plate layer 31 further comprises a second connection unit 312, and the second connection unit 312 connects adjacent second cartilage units 311.
In a preferred embodiment, the material of the second connection unit 312 is a high molecular polymer.
In a preferred embodiment, the high molecular polymer is selected from at least one of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid.
In a preferred embodiment, as shown in fig. 4, the disc body 2 has a shape of a circular hollow cylinder, and the nucleus cavity 21 is a hollow circular hollow cylinder.
In a preferred embodiment, as shown in fig. 1, 3 and 4, the disc body 2 comprises several layers of disc body layers 22. The disc body 2 mimics the annulus fibrosus of an intervertebral disc.
In a preferred embodiment, as shown in fig. 3 and 4, the disc body layer 22 includes a plurality of disc body members 221 arranged in concentric circles.
In a preferred embodiment, as shown in fig. 4, the artificial disc tissue includes a first disc body unit 2211 and a second disc body unit 2212 in the same disc body layer, the material of the first disc body unit 2211 is hydrogel mixed with fibroblast and fibroblast growth factor, and the material of the second disc body unit 2212 is high polymer; each of the disc body layers is alternately arranged with the first disc body units 2211 and the second disc body units 2212 in order from the outside to the inside. The high molecular polymer provides the required mechanical strength, and the hydrogel mixed with the fibroblast and the fibroblast growth factor enables the artificial intervertebral disc tissue to have biological activity, promotes the regeneration of the intervertebral disc, prolongs the service life of the intervertebral disc and delays the degeneration of the intervertebral disc and related tissues.
In a preferred embodiment, the outermost disc body unit is a hydrogel mixed with fibroblasts and fibroblast growth factors.
In a preferred embodiment, the density of fibroblasts is 4000 to 6000 fibroblasts/mm 3 。
In a preferred embodiment, the concentration of fibroblast growth factor is between 10 and 100000ng/uL.
In a preferred embodiment, the high molecular polymer is selected from at least one of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid.
In a preferred embodiment, as shown in fig. 5, the nucleus pulposus unit 4 is in the shape of an egg. The design allows the nucleus unit to deform during disc compression and rebound after compression is removed. The design allows the artificial disc tissue to have the mobility of the spinal unit.
In a preferred embodiment, the material of the nucleus pulposus unit 4 is a hydrogel, including but not limited to GelMA.
The second aspect of the present invention provides the above method for constructing an artificial intervertebral disc tissue, comprising the steps of:
1) Constructing a lumbar model;
the lumbar model can be generally constructed according to CT data and/or MRI data, and the data can be imported into software such as three-dimensional reconstruction design software such as E3D and the like in the construction process, and registration fusion is carried out, so that the lumbar model is constructed;
2) Determining a excision range, simulating excision on the lumbar model to obtain an excised lumbar model, and taking excision parts as an initial artificial intervertebral disc tissue model;
in determining the range of resection, the occurrence of high collapse, calcification, herniated disc is typically selected in the model as the resection target, and location and size are determined in the model.
3) And constructing a first cartilage plate model, an intervertebral disc body model, a second cartilage plate model and a nucleus pulposus unit model by taking the outer contour of the initial artificial intervertebral disc tissue model as a reference, wherein the constructed intervertebral disc body model is provided with a nucleus pulposus cavity for accommodating the nucleus pulposus unit model.
In a preferred embodiment, the first cartilage plate layer model is constructed as a first cartilage plate layer model of several layers, the second cartilage plate layer model is constructed as a second cartilage plate layer model of several layers, and the disc body model is constructed as a disc body layer of several layers.
In a preferred embodiment, the first cartilage plate layer model is constructed as a plurality of parallel first cartilage unit models, the second cartilage plate layer model is constructed as a plurality of parallel second cartilage unit models, and the disc body layer model is constructed as a plurality of disc body unit models arranged in concentric circles.
In a preferred embodiment, the first cartilage unit model intersection angle α of the build adjacent layer is 30-90 °.
In a preferred embodiment, a first connection element model is constructed on a first cartilage plate layer model, connecting adjacent first cartilage element models.
In a preferred embodiment, the second cartilage unit model intersection angle β of the build adjacent layer is 30-90 °.
In a preferred embodiment, a second connection element model is constructed on the second cartilage plate layer model, connecting adjacent second cartilage element models.
In a preferred embodiment, the model of the disc body is constructed in the shape of a circular hollow cylinder, and the nucleus cavity is the cavity of the circular hollow cylinder.
In a preferred embodiment, the model of the nucleus unit is constructed in the shape of an egg.
In a preferred embodiment, the construction method further comprises: a fixation unit model is constructed that runs through the disc body model from one side to the other and through the nucleus unit model.
A third aspect of the present invention provides a method for preparing the above-described artificial disc tissue, the method comprising: and constructing and obtaining an artificial intervertebral disc tissue model according to the construction method of the artificial intervertebral disc tissue model, and preparing the artificial intervertebral disc tissue.
In a preferred embodiment, the artificial disc tissue is prepared by 3D printing.
According to the construction method of the artificial intervertebral disc tissue model, the artificial intervertebral disc tissue model is constructed and obtained, a printing path is set, a computer STL file is generated, then the file is transferred to a program of a 3D printer, and printing is started.
A computer readable storage medium having stored thereon a computer program which when executed by a processor performs the steps of the method of constructing an artificial disc tissue model as described above, or of the method of preparing an artificial disc tissue as described above.
The computer-readable storage medium, as will be appreciated by one of ordinary skill in the art: all or part of the steps for implementing the method embodiments described above may be performed by computer program related hardware. The aforementioned computer program may be stored in a computer readable storage medium. The program, when executed, performs steps including the method embodiments described above; and the aforementioned storage medium includes: various media that can store program code, such as ROM, RAM, magnetic or optical disks.
An apparatus, comprising: the device comprises a processor and a memory, wherein the memory is used for storing a computer program, and the processor is used for executing the computer program stored in the memory, so that the device executes the steps of the method for constructing the artificial intervertebral disc tissue model or the steps of the method for preparing the artificial intervertebral disc tissue.
Example 1
An artificial intervertebral disc tissue sequentially comprises a first cartilage plate 1, an intervertebral disc body 2 and a second cartilage plate 3 from top to bottom, the artificial intervertebral disc tissue further comprises a nucleus pulposus unit 4, the intervertebral disc body 2 is provided with a nucleus pulposus cavity 21 for accommodating the nucleus pulposus unit 4, and the whole artificial intervertebral disc tissue is cylindrical.
The first cartilage plate 1 comprises 2 layers of first cartilage plate layers 11, theThe first cartilage plate layer 11 comprises a plurality of parallel first cartilage units 111, the number of the parallel first cartilage units is determined according to the size of the intervertebral disc, the crossing angle alpha of the first cartilage units 111 of the adjacent layers is 90 degrees, the materials of the adjacent first cartilage units are polycaprolactone and hydrogel mixed with chondrocyte and chondrocyte growth factors, the chondrocyte growth factors are bone morphogenetic protein-4/7, and the density of the chondrocyte is 5000-8000/mm 3 The concentration of the chondrocyte growth factor is 10-100000 ng/uL; the first cartilage plate layer 11 further comprises a first connection unit 112, the first connection unit 112 is connected with the adjacent first cartilage unit 111, and the first connection unit 112 is made of polycaprolactone.
The disc body 2 is in a shape of a circular hollow cylinder, the nucleus pulposus cavity 21 is a cavity of the circular hollow cylinder, the disc body 2 comprises a plurality of disc body layers 22, the number of layers is determined according to the size of the disc, the disc body layers 22 comprise a plurality of disc body components 221 which are arranged in concentric circles, the number of the disc body components is determined according to the size of the disc, the artificial disc tissues comprise a first disc body unit 2211 and a second disc body unit 2212 in the same disc body layer, the material of the first disc body unit 2211 is hydrogel mixed with fibroblast and fibroblast growth factor, the material of the second disc body unit 2212 is a high polymer, and the high polymer is polycaprolactone; each disc body layer is alternately arranged with a first disc body unit 2211 and a second disc body unit 2212 from outside to inside, the outermost disc body unit is hydrogel mixed with fibroblast and fibroblast growth factor, and the density of the fibroblast is 5000/mm 3 The concentration of fibroblast growth factor was 400ng/uL.
The nucleus pulposus unit 4 is in the shape of an egg, and the material of the nucleus pulposus unit 4 is hydrogel.
The second cartilage plate 3 comprises 2 layers of second cartilage plate layers 31, the second cartilage plate layers 31 comprising a plurality of parallel second cartilage units 311, the number of parallel second cartilage units being determined according to the disc size, the second of the adjacent layersThe crossing angle beta of the cartilage units 311 is 90 degrees, the materials of the adjacent second cartilage units are respectively polycaprolactone and hydrogel mixed with chondrocyte and chondrocyte growth factors, the chondrocyte growth factors are bone morphogenetic protein-4/7, and the density of the chondrocyte is 5000-8000/mm 3 The concentration of the chondrocyte growth factor is 10-100000 ng/uL; the second cartilage plate layer 31 further comprises a second connection unit 312, the second connection unit 312 connects adjacent second cartilage units 311, and the second connection unit 312 is made of polycaprolactone.
The preparation method of the artificial intervertebral disc tissue comprises the following steps:
1) Constructing a lumbar model;
the lumbar model can be generally constructed according to CT data and/or MRI data, and the data can be imported into software such as three-dimensional reconstruction design software such as E3D and the like in the construction process, and registration fusion is carried out, so that the lumbar model is constructed;
2) Determining a excision range, simulating excision on the lumbar model to obtain an excised lumbar model, and taking excision parts as an initial artificial intervertebral disc tissue model;
in determining the range of resection, the occurrence of high collapse, calcification, herniated disc is typically selected in the model as the resection target, and location and size are determined in the model.
3) Constructing a first cartilage plate model, an intervertebral disc body model, a second cartilage plate model and a nucleus pulposus unit model by taking the outer contour of the initial artificial intervertebral disc tissue model as a reference, wherein the constructed intervertebral disc body model is provided with a nucleus pulposus cavity for accommodating the nucleus pulposus unit model;
constructing a first cartilage plate layer model with a first cartilage plate layer model of 2 layers, constructing a second cartilage plate layer model with a second cartilage plate layer model of 2 layers, constructing an intervertebral disc body model into a plurality of intervertebral disc body layers, and determining the number of layers according to the size of an intervertebral disc.
The method comprises the steps of constructing a first cartilage plate layer model into a plurality of parallel first cartilage unit models, determining the number of the parallel first cartilage unit models according to the size of an intervertebral disc, constructing a second cartilage plate layer model into a plurality of parallel second cartilage unit models, determining the number of the parallel second cartilage unit models according to the size of the intervertebral disc, and constructing an intervertebral disc body part model into a plurality of concentric circle arranged intervertebral disc body part models, wherein the number of the intervertebral disc part models is determined according to the size of the intervertebral disc.
The first cartilage unit model of the constructed adjacent layer has an intersection angle alpha of 90 deg.. And constructing a first connection unit model on the first cartilage plate layer model, and connecting adjacent first cartilage unit models. The second cartilage unit model of the constructed adjacent layer has a 90 ° intersection angle beta. And constructing a second connection unit model on the second cartilage plate layer model, and connecting adjacent second cartilage unit models.
The model of the intervertebral disc body is constructed to be in the shape of a circular hollow cylinder, and the nucleus cavity is a cavity of the circular hollow cylinder. The nucleus pulposus unit model is constructed in the shape of an egg.
4) Artificial disc tissue was prepared by 3D printing.
According to the construction method of the artificial intervertebral disc tissue model, the artificial intervertebral disc tissue model is constructed and obtained, a printing path is designed, a computer STL file is generated, then the file is transferred to a program of a 3D printer, and printing is started.
The above embodiments are merely illustrative of the principles of the present invention and its effectiveness, and are not intended to limit the invention. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the invention. Accordingly, it is intended that all equivalent modifications and variations of the invention be covered by the claims, which are within the ordinary skill of the art, be within the spirit and scope of the present disclosure.
Claims (16)
1. An artificial intervertebral disc tissue, which is characterized by comprising a first cartilage plate (1), an intervertebral disc body (2) and a second cartilage plate (3) from top to bottom in sequence, and further comprising a nucleus pulposus unit (4), wherein the intervertebral disc body (2) is provided with a nucleus pulposus cavity (21) for accommodating the nucleus pulposus unit (4); the first cartilage plate (1) comprises a plurality of layers of first cartilage plate layers (11); the first cartilage plate layer (11) comprises a plurality of parallel first cartilage units (111);
the method also comprises the following technical characteristics:
feature 2), the second cartilage plate (3) comprising several layers of second cartilage plate layers (31);
feature 4), the disc body (2) comprising several layers of disc body layers (22);
31 In feature 2), the second cartilage plate layer (31) comprises a number of parallel second cartilage units (311);
51 In feature 4), the disc body layer (22) includes a plurality of disc body members (221) arranged in concentric circles;
211 A first cartilage unit (111) crossing angle alpha of 30-90 DEG for adjacent layers;
214 -the first cartilage sheet layer (11) further comprises a first connection unit (112), the first connection unit (112) connecting adjacent first cartilage units (111);
311 In the feature 31), the crossing angle beta of the second cartilage units (311) of the adjacent layers is 30-90 degrees;
314 -feature 31), the second cartilage plate layer (31) further comprising a second connection unit (312), the second connection unit (312) connecting adjacent second cartilage units (311);
511 In the feature 51), the artificial disc tissue includes a first disc body unit (2211) and a second disc body unit (2212) in the same disc body layer, the material of the first disc body unit (2211) is hydrogel mixed with fibroblast and fibroblast growth factor, and the material of the second disc body unit (2212) is high polymer; each disc body layer is alternately arranged with a first disc body unit (2211) and a second disc body unit (2212) in sequence from outside to inside.
2. The artificial disc tissue of claim 1, further comprising at least one of the following technical features:
1) The artificial intervertebral disc tissue is cylindrical as a whole;
2) The intervertebral disc body (2) is in the shape of a circular ring hollow cylinder, and the nucleus pulposus cavity (21) is a cavity of the circular ring hollow cylinder;
3) The nucleus pulposus unit (4) is in the shape of an egg;
4) The material of the nucleus pulposus unit (4) is hydrogel.
3. The artificial disc tissue of claim 1, further comprising at least one of the following technical features:
212 The materials of adjacent first cartilage units in the same first cartilage plate layer are respectively high molecular polymers and hydrogels mixed with chondrocytes and chondrocyte growth factors;
213 The materials of the adjacent first cartilage plate layers are respectively high molecular polymers and hydrogels mixed with chondrocytes and chondrocyte growth factors.
4. The artificial disc tissue of claim 1, further comprising at least one of the following technical features:
312 In feature 31), the materials of adjacent second cartilage units in the same second cartilage plate layer are respectively a high molecular polymer and a hydrogel mixed with chondrocytes and chondrocyte growth factors;
313 In feature 31), the materials of adjacent second chondrocyte layers are a polymer and a hydrogel mixed with chondrocytes and chondrocyte growth factors, respectively.
5. The artificial disc tissue of claim 3, further comprising at least one of the following technical features:
2121 In feature 212), the high molecular polymer is at least one selected from the group consisting of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid;
2122 In feature 212), the chondrocyte growth factor is selected from one or more of bone morphogenic protein-4/7 and combined transcriptional growth β;
2123 In feature 212), the chondrocyte density is 5000-8000/mm 3 ；
2124 In feature 212), the concentration of chondrocyte growth factor is 10-100000 ng/uL;
2131 213) the high molecular polymer is at least one selected from the group consisting of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid;
2132 213) the chondrocyte growth factor is selected from one or more of bone morphogenic protein-4/7 and combined transcriptional growth β;
2133 213) the chondrocyte density is 5000-8000/mm 3 ；
2134 In feature 213), the concentration of chondrocyte growth factor is 10-100000 ng/uL.
6. The artificial disc tissue of claim 1, wherein 2141) the feature 214) the material of the first connection unit (112) is a high molecular polymer.
7. The artificial disc tissue of claim 4, further comprising at least one of the following technical features:
3121 In feature 312), the high molecular polymer is at least one selected from the group consisting of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid;
3122 Characterized) in feature 312), the chondrocyte growth factor is selected from one or more of bone morphogenic protein-4/7 and combined transcriptional growth β;
3123 In feature 312), the chondrocyte density is 5000-8000/mm 3 ；
3124 Characteristic 312), the concentration of the chondrocyte growth factor is 10-100000 ng/uL;
3131 313) the high molecular polymer is selected from at least one of polycaprolactone, polylactic acid-glycolic acid copolymer, and polylactic acid;
3132 313) the chondrocyte growth factor is selected from one or more of bone morphogenic protein-4/7 and combined transcriptional growth β;
3133 313) the density of chondrocytes is 5000-8000/mm 3 ；
3134 313) the concentration of chondrocyte growth factor is 10-100000 ng/uL.
8. The artificial disc tissue of claim 1, further comprising at least one of the following features:
3141 314), the material of the second connection unit (312) is a high molecular polymer;
5111 Feature 511), the outermost disc body unit is a hydrogel mixed with fibroblasts and fibroblast growth factors;
5112 In feature 511), the density of fibroblasts is 4000 to 6000 fibroblasts/mm 3 ；
5113 In feature 511), the concentration of fibroblast growth factor is 10-100000 ng/uL;
5114 In feature 511), the high molecular polymer is at least one selected from the group consisting of polycaprolactone, a polylactic acid-glycolic acid copolymer, and polylactic acid.
9. The method of constructing an artificial disc tissue according to any one of claims 1 to 8, comprising the steps of:
1) Constructing a lumbar model;
2) Determining a excision range, simulating excision on the lumbar model to obtain an excised lumbar model, and taking excision parts as an initial artificial intervertebral disc tissue model;
3) And constructing a first cartilage plate model, an intervertebral disc body model, a second cartilage plate model and a nucleus pulposus unit model by taking the outer contour of the initial artificial intervertebral disc tissue model as a reference, wherein the constructed intervertebral disc body model is provided with a nucleus pulposus cavity for accommodating the nucleus pulposus unit model.
10. The method of constructing artificial disc tissue according to claim 9, further comprising at least one of the following technical features:
1) In step 1), constructing the lumbar model from CT data and/or MRI data;
2) In the step 3), a first cartilage plate layer model is built to be a first cartilage plate layer model with a plurality of layers, a second cartilage plate layer model is built to be a second cartilage plate layer model with a plurality of layers, and an intervertebral disc body model is built to be an intervertebral disc body layer with a plurality of layers;
3) In the step 3), constructing an intervertebral disc body model to be in the shape of a circular ring hollow cylinder, wherein the nucleus cavity is a cavity of the circular ring hollow cylinder;
4) In step 3), a nucleus unit model is constructed in the shape of an egg.
11. The method of constructing an artificial disc tissue according to claim 10, wherein in the feature 2), the first cartilage plate layer model is constructed as a plurality of parallel first cartilage unit models, the second cartilage plate layer model is constructed as a plurality of parallel second cartilage unit models, and the disc body part model is constructed as a plurality of disc body part models arranged in concentric circles.
12. The method of constructing artificial disc tissue of claim 11, further comprising at least one of the following technical features:
1) Constructing a first cartilage unit model of an adjacent layer, wherein the crossing angle alpha of the first cartilage unit model is 30-90 degrees;
2) Constructing a first connection unit model on the first cartilage plate layer model, and connecting adjacent first cartilage unit models;
3) Constructing a second cartilage unit model of an adjacent layer, wherein the crossing angle beta of the second cartilage unit model is 30-90 degrees;
4) And constructing a second connection unit model on the second cartilage plate layer model, and connecting adjacent second cartilage unit models.
13. The method of preparing artificial disc tissue according to any one of claims 1 to 6, wherein the method of preparing comprises: the method for constructing an artificial intervertebral disc tissue model according to any one of claims 9 to 12, wherein the artificial intervertebral disc tissue model is constructed and obtained, and the artificial intervertebral disc tissue is prepared.
14. The method of preparing artificial disc tissue according to claim 13, wherein the artificial disc tissue is prepared by 3D printing.
15. A computer-readable storage medium, on which a computer program is stored, characterized in that the program, when being executed by a processor, implements the steps of the method of constructing an artificial disc tissue model according to any one of claims 9 to 12, or the steps of the method of preparing an artificial disc tissue according to claim 11 or 12.
16. An apparatus, comprising: a processor and a memory for storing a computer program, the processor being adapted to execute the computer program stored by the memory to cause the apparatus to perform the steps of the method of constructing an artificial disc tissue model according to any one of claims 9 to 12 or the steps of the method of preparing an artificial disc tissue according to claim 13 or 14.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5865845A (en) * | 1996-03-05 | 1999-02-02 | Thalgott; John S. | Prosthetic intervertebral disc |
| CN101909548A (en) * | 2008-01-17 | 2010-12-08 | 斯恩蒂斯有限公司 | An expandable intervertebral implant and associated method of manufacturing the same |
| CN107496060A (en) * | 2017-08-09 | 2017-12-22 | 国家纳米科学中心 | A kind of three-dimensional artificial total spinal disc and its preparation method and application |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69522060T2 (en) * | 1994-09-08 | 2002-05-29 | Stryker Technologies Corp | Intervertebral disc core made of hydrogel |
| US6482234B1 (en) * | 2000-04-26 | 2002-11-19 | Pearl Technology Holdings, Llc | Prosthetic spinal disc |
| US6468311B2 (en) * | 2001-01-22 | 2002-10-22 | Sdgi Holdings, Inc. | Modular interbody fusion implant |
| CA2595266C (en) * | 2005-01-19 | 2010-12-21 | Casey K. Lee | Elastomeric intervertebral disc prosthesis |
| US20070179618A1 (en) * | 2006-01-31 | 2007-08-02 | Sdgi Holdings, Inc. | Intervertebral prosthetic disc |
| WO2008022206A2 (en) * | 2006-08-15 | 2008-02-21 | Motionback Llc | Spinal implant |
| CN102727329B (en) * | 2012-06-28 | 2015-01-07 | 深圳清华大学研究院 | Artificial cervical intervertebral disc |
| CN103190969A (en) * | 2013-05-02 | 2013-07-10 | 山东威高骨科材料有限公司 | Artificial intervertebral disc |
| CN204542472U (en) * | 2015-01-30 | 2015-08-12 | 马盟 | A kind of artificial intervertebral disk |
| CN106821554A (en) * | 2015-12-03 | 2017-06-13 | 重庆智寰传动科技发展有限公司 | A kind of artificial intervertebral disk |
| CN107049563B (en) * | 2017-04-19 | 2020-02-07 | 北京爱康宜诚医疗器材有限公司 | Intervertebral disc prosthesis |
| CN208756262U (en) * | 2018-01-20 | 2019-04-19 | 西安市红会医院 | A kind of 3D printing cervical artificial disc prosthese |
| CN110025409B (en) * | 2019-04-17 | 2021-03-23 | 东南大学 | Elastic interbody fusion cage with magnesium powder function enhanced high polymer material porous structure and preparation method thereof |
| CN112294502A (en) * | 2020-10-30 | 2021-02-02 | 四川大学华西医院 | Assembly type total intervertebral disc prosthesis based on 3D printing technology and manufacturing method thereof |
-
2021
- 2021-03-22 CN CN202110301861.3A patent/CN113143548B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5865845A (en) * | 1996-03-05 | 1999-02-02 | Thalgott; John S. | Prosthetic intervertebral disc |
| CN101909548A (en) * | 2008-01-17 | 2010-12-08 | 斯恩蒂斯有限公司 | An expandable intervertebral implant and associated method of manufacturing the same |
| CN107496060A (en) * | 2017-08-09 | 2017-12-22 | 国家纳米科学中心 | A kind of three-dimensional artificial total spinal disc and its preparation method and application |
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