CN113141766B

CN113141766B - Electrophysiological experiment shielding box

Info

Publication number: CN113141766B
Application number: CN202110411204.4A
Authority: CN
Inventors: 李沅衡; 黄剑平; 汪圆圆; 余潜衡远; 王萍; 李光林
Original assignee: Shenzhen Institute of Advanced Technology of CAS
Current assignee: Shenzhen Institute of Advanced Technology of CAS
Priority date: 2021-04-16
Filing date: 2021-04-16
Publication date: 2022-10-14
Anticipated expiration: 2041-04-16
Also published as: WO2022217951A1; CN113141766A

Abstract

The application discloses electrophysiological experiment shielded cell relates to electrophysiological experiment shielded cell technical field. The device is suitable for electrophysiological experiments of rodent live animals, can reliably fix the live animals, and is very convenient to operate. The electrophysiological experiment shielding box comprises a box body, wherein a box door is arranged on one side surface of the box body; an operation platform is arranged in the box body, an animal head fixing mechanism is arranged on the operation platform, and the animal head fixing mechanism comprises a support assembly, at least one animal tooth fixing block and at least two animal ear fixing shafts, wherein the at least one animal tooth fixing block and the at least two animal ear fixing shafts are arranged on the support assembly. The application is used for improving the performance of the electrophysiological experiment shielding box.

Description

Electrophysiological experiment shielding box

Technical Field

The application relates to the technical field of electrophysiological acquisition instruments and equipment, in particular to an electrophysiological experiment shielding box.

Background

Electrophysiological techniques refer to techniques for stimulating a living body with various forms of energy (electricity, sound, etc.), measuring, recording and analyzing electrical phenomena (bioelectricity) occurring in the living body and electrical characteristics of the living body. The very weak electrical activity of cells in the body can be recorded from the body surface of a human or an animal through the electrophysiological technology, and the clinical diagnosis is facilitated through the analysis of the electrical activity. The electrophysiological experiments are important components in the exercise and physiology experiment course, and medical personnel further learn the activities and characteristics of a living body through the electrophysiological experiments. When carrying out the electrophysiological experiment, generally can adopt electrophysiological experiment shielded cell to shield in order to obtain better experimental effect external electromagnetic interference signal.

The existing electrophysiological experiment shielding box only aims at animal nerves and body tissues generally, and does not specially aim at the electrophysiological experiment shielding box of the living animal, so that the electrophysiological experiment of the living animal is very inconvenient to carry out.

Disclosure of Invention

The embodiment of the application provides an electrophysiological experiment shielded cell, is applicable to rodent live body animal's electrophysiological experiment, can reliably fix the live body animal, and the operation is very convenient.

In order to achieve the above object, an embodiment of the present application provides a shielding box for electrophysiological experiments, which includes a box body, wherein a box door is arranged on one side surface of the box body; be equipped with operation platform in the box, the last animal head fixed establishment that is equipped with of operation platform, animal head fixed establishment includes the bracket component and sets up at least one animal tooth fixed block and two at least animal ear fixed axles on the bracket component.

Further, the bracket assembly comprises a U-shaped bracket arranged along the horizontal direction, and a first upright post and a second upright post arranged along the vertical direction; the first upright posts are respectively positioned on the corresponding horizontal sections of the U-shaped bracket; the second upright post is positioned on the arc-shaped section of the U-shaped bracket; the opening of the U-shaped bracket faces the box door; one ends of the first upright column and the second upright column are connected with the operating platform, and the other ends of the first upright column and the second upright column are connected with the U-shaped support in a sliding manner; the U-shaped support can move up and down along the first upright post and the second upright post.

Furthermore, the U-shaped support, the first upright post and the second upright post are square tubes; the U-shaped support is provided with a first through hole which is communicated up and down, the side length of the first through hole is matched with the side lengths of the first upright post and the second upright post, and the U-shaped support is sleeved on the first upright post and the second upright post and can slide up and down along the first upright post and the second upright post; a plurality of first knobs are connected to the outer side wall of the U-shaped support in a threaded mode, and the threaded sections of the first knobs extend into the U-shaped support and are abutted to the first upright posts or the second upright posts.

Further, the inside wall of first stand is equipped with the second through-hole, the first end of animal ear's fixed axle passes get into behind the second through-hole in the cavity of first stand, threaded connection second knob on the preceding lateral wall of first stand or the back lateral wall, the screw thread section of second knob stretch into in the first stand and with animal ear's fixed axle butt.

Further, the inside wall of second stand is equipped with the third through-hole, the first end of animal tooth fixed block is equipped with the draw-in groove that is used for joint animal tooth, the second end of animal tooth fixed block passes get into behind the third through-hole in the cavity of second stand, threaded connection third knob on the left side wall of second stand or the right side wall, the screw thread section of third knob stretch into in the second stand and with animal tooth fixed block butt.

Furthermore, the operating platform is connected with the box body in a sliding mode and can move towards the direction close to or away from the box door.

Furthermore, the box body is provided with sliding grooves extending along the horizontal direction on two vertical wall surfaces located on two sides of the door body, and sliding blocks matched with the sliding grooves are arranged on two sides of the operating platform.

Further, a camera is arranged in the box body, and the camera can shoot and record the reaction of the experimental object in real time.

Furthermore, the top wall of the box body adopts a perspective shielding net.

Furthermore, a flexible wire crocodile clip for fixing the electrode assembly is arranged in the box body.

Compared with the prior art, the application has the following beneficial effects:

the operating platform is arranged in the electrophysiological experiment shielding box, the animal head fixing mechanism is arranged on the operating platform and comprises a bracket component, an animal tooth fixing block and at least two animal ear fixing shafts, wherein the animal tooth fixing block and the at least two animal ear fixing shafts are arranged on the bracket component; before the experiment, the teeth of the living animal are fixed in the animal tooth fixing block, and the animal ear fixing shaft is inserted into the auditory canal of the living animal, so that the operation is very convenient and the fixation is very reliable.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic external view of a shielding box for electrophysiological experiments according to an embodiment of the present disclosure;

FIG. 2 is a schematic structural diagram of the electrophysiological experiment shielding box of the embodiment of the present application with the door removed;

FIG. 3 is a schematic structural diagram of an operation platform in the electrophysiological experiment shielding box in the embodiment of the present application

FIG. 4 is a schematic structural diagram of an animal head fixing mechanism in a shielding box for electrophysiological experiments according to an embodiment of the present application;

FIG. 5 is a schematic view of a connection structure of a first upright post and an animal ear fixing shaft in the shielding box for electrophysiological experiments according to the embodiment of the present application;

FIG. 6 is a schematic view of a connection structure of a second upright post and an animal tooth fixing block in the shielding box for electrophysiological experiments according to the embodiment of the present application;

FIG. 7 is a schematic structural diagram of a top wall of a box body in the electrophysiological experiment shielding box of the embodiment of the present application;

fig. 8 is a schematic structural diagram of the left side wall in the shielding box for electrophysiological experiments in the embodiment of the present application.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

Referring to fig. 1 and 2, an embodiment of the present application provides a shielding box for electrophysiological experiments of experiments on living rodents, which includes a box body 1, and a box door 2 is arranged on a front side surface of the box body 1. The door 2 is pivotably installed on the cabinet 1, and selectively opens or closes the cabinet 1. The box body 1 and the box door 2 are locked by a box door lock catch 13 and a rotary lock knob 14. An operation platform 3 made of bakelite is arranged in the box body 1, and the upper part of the operation platform 3 is wrapped by conductive rubber. An animal head fixing mechanism 4 is arranged on the operating platform 3. Referring to fig. 3, the edge of the operation platform 3 is engraved with a scale 32 that provides a proportional reference for the experimental recordings. Referring to fig. 4, the animal head fixing mechanism 4 includes a bracket assembly 41, an animal tooth fixing block 42 disposed on the bracket assembly 41, and at least two animal ear fixing shafts 43. Before the brain electrophysiological experiment is carried out, only the teeth of the tested living animal are required to be fixed in the animal tooth fixing block 42, and the animal ear fixing shaft 43 is required to be inserted into the auditory canal of the tested living animal, so that the operation is very convenient and the fixation is very reliable.

When the living animal neuroelectrophysiology detection is carried out, no matter the nerve is central or peripheral, the noise interference caused by the surrounding environment to the electrophysiology signal obviously influences the signal acquisition, and the signal quality is reduced, so that the box body 1 and the box door 2 in the embodiment of the application are both made of bakelite materials, and the outer layer of the box body is wrapped by conductive rubber.

Referring to fig. 1 and 2, the box top wall 103 is pivotally connected to the box left side wall 104, and the structure can also realize the closing or opening of the box 1, which meets different experimental requirements. In order to facilitate the closing or opening of the box body, the box door 2 and the box body top wall 103 are both provided with handles 8.

Referring to fig. 2, the case left side wall 104 and the case right side wall 105 are provided with electrode lead inlets 9 for passing electrode leads (not shown). The rear side wall of the box body 1 is provided with a gas anesthesia catheter inlet 10 for a gas anesthesia catheter (not shown) to pass through so as to ensure that the experimental animal is in an anesthesia state in the whole process.

Referring to fig. 4, in some embodiments, the number of the animal tooth fixing blocks 42 is one, the number of the animal ear fixing shafts 43 is two, and the two animal ear fixing shafts 43 are disposed to face each other, whereby the structure of the animal head fixing mechanism 4 is simpler.

In other embodiments, the number of the animal tooth fixing blocks 42 is multiple, and the number of the animal ear fixing shafts 43 is more than two, so that when one of the animal tooth fixing blocks 42 or the animal ear fixing shafts 43 is damaged, only the spare animal tooth fixing block 42 or the spare animal ear fixing shaft 43 needs to be replaced, and the experimental process cannot be delayed due to equipment damage.

Referring to fig. 4-7, in some embodiments, the bracket assembly 41 includes a U-shaped bracket 411 disposed in a horizontal direction and two first uprights 412 and one second upright 413 disposed in a vertical direction. Wherein, two first vertical columns 412 are respectively located on the corresponding horizontal sections of the U-shaped bracket 411, and the second vertical column 413 is located on the arc section of the U-shaped bracket 411. Referring to fig. 2,U the opening of the bracket 411 is facing the door 2. One end of each of the first column 412 and the second column 413 is connected to the operation platform 3, the other end of each of the first column 412 and the second column 413 is slidably connected to the U-shaped bracket 411, and the U-shaped bracket 411 can move up and down along the first column 412 and the second column 413.

With continued reference to fig. 4-7, in some embodiments, U-shaped bracket 411, first upright 412, and second upright 413 are square tubes. The U-shaped bracket 411 is provided with a first through hole 4111 which is through from top to bottom, the side length of the first through hole 4111 is matched with the side lengths of the first upright column 412 and the second upright column 413, and the U-shaped bracket 411 is sleeved on the first upright column 412 and the second upright column 413 and can slide up and down along the first upright column 412 and the second upright column 413. A plurality of first knobs 414 are connected to the outer side wall of the U-shaped bracket 411 in a threaded manner, and the threaded sections of the first knobs 414 extend into the U-shaped bracket 411 and abut against the first upright posts 412 or the second upright posts 413. Loosening the first knob 414, can adjust the height of U-shaped support 411, and screwing the second knob 414 can fix U-shaped support 411 in preset position, from this, the living animal of this application embodiment can be applicable to different heights, and application scope is wider.

Referring to fig. 4-6, in some embodiments, the inner sidewall of the first upright 412 is provided with a second through hole 4121, the first end of the animal ear fixing shaft 43 passes through the second through hole 4121 and enters the cavity of the first upright 412, and the animal ear fixing shaft 43 is inserted into the ear canal of the living animal after the living animal is fixed. The front side wall of the first upright post 412 is in threaded connection with a second knob 415, and the threaded section of the second knob 415 extends into the first upright post 412 and abuts against the animal ear fixing shaft 43. Not hard up second knob 415, can adjust the distance between two animal ear fixed axles 43, and the second knob 415 of screwing can fix animal ear fixed axle 43 in preset position, from this, the living animal of different two footpaths can be applicable to this application embodiment, and application scope is wider.

With continued reference to fig. 4 to 6, on the premise of adopting the technical solution that the outer sidewall of the first upright 412 is screwed to the second knob 415, in some embodiments, the inner sidewall of the second upright 413 is provided with a third through hole 4131, the first end of the animal tooth fixing block 42 is provided with a slot 421 for clamping the animal tooth, and after the living animal is fixed, the tooth of the living animal is clamped into the slot 421. The second end of the animal tooth fixing block 42 passes through the third through hole 4131 and then enters the cavity of the second upright column 413, the left side wall of the second upright column 413 is in threaded connection with the third knob 416, and the threaded section of the third knob 416 extends into the second upright column 413 and abuts against the animal tooth fixing block 42. The third knob 416 is loosened, the front and back displacement of the animal tooth fixing block 42 can be adjusted, and the animal tooth fixing block 42 can be fixed at a preset position by screwing the third knob 416, so that the animal tooth fixing block can be suitable for living animals with different head lengths, and the application range is wider.

Referring to fig. 2, in the premise of adopting the technical solution that the outer sidewall of the first upright 412 is screwed with the second knob 415 and the outer sidewall of the second upright 413 is screwed with the third knob 416, in some embodiments, the operation platform 3 is slidably connected with the box body 1 and can move toward or away from the box door 2. From this, the position of setting up the three dimension of animal head fixed establishment 4 on operation platform 3 all can be adjusted, is favorable to once only adjusting to target in place, carries out unnecessary removal to being carried out by experimental animals in the reduction experiment, has reduced the influence of jamming to experimental animals, and it is more convenient to use.

In some embodiments, the animal head fixing mechanism 4 and the operation platform 3 are detachably connected, so that the animal head fixing mechanism 4 is convenient to replace when damaged, and other types of experiments can be performed after the animal head fixing mechanism 4 is removed, and therefore, the application range of the embodiment of the application is wider. Specifically, referring to fig. 3, a fixing frame slot 31 for fixing the animal head fixing mechanism 4 is disposed on the operation platform 3, and a side length of the fixing frame slot 31 is adapted to a side length of the first upright 412 and a side length of the second upright 413.

With continued reference to fig. 2, in some embodiments, the two vertical wall surfaces of the box body 1 on the two sides of the door body are respectively provided with a sliding groove 101 extending along the horizontal direction, and the two sides of the operation platform 3 are provided with sliding blocks (not shown) adapted to the sliding grooves 101. In other embodiments, the operation platform 3 and the box body 1 can also be slidably connected through a sliding rail assembly.

When the electrophysiological signal of a living animal is detected, the nerve or muscle tissue to be detected needs to be separated under an anesthesia state, and then the nerve or muscle tissue needs to be recorded or stimulated, but the existing shielding box cannot realize effective synchronous recording and observation. Therefore, referring to fig. 8, in some embodiments, a camera 5 is provided in the housing 1, the camera 5 can shoot and record the reaction of the experimental subject in real time, and by connecting the camera 5 with a computer display outside the housing 1, the reaction of the experimental animal can be observed on the display screen at the same time. Specifically, a camera card slot 11 for installing the camera 5 and a camera data line hole 12 for passing through a data line are arranged on the left side wall 104 and the right side wall 105 of the box body. Therefore, the embodiment of the application can observe the specific activity state of the animal while recording so as to adjust in real time, and the electrophysiological signals and the animal tissue reaction are reflected on the computer screen in real time through the camera 5, so that the aims of effectively shielding the interference of surrounding signals and observing the experiment in real time are fulfilled. For better clarity of observation, the cameras 5 may be high-definition digital cameras, and the number of the cameras 5 may be selected according to actual needs. Illustratively, the number of the cameras 5 may be two, and the two cameras are respectively positioned at the upper left and the upper right of the case 1.

In some embodiments, for easier observation, the top wall 103 of the box body is a perspective shielding net, so that the experimenter can directly observe the top of the box body 1 in real time when acquiring signals of the animals.

Because the electrode assembly in the prior art cannot be effectively fixed during recording or stimulation, the movement of muscles during stimulation often causes the displacement of the electrodes in the electrode assembly during recording and stimulation, which affects the progress of the experiment, and when the recording or stimulation involves a plurality of nerve or muscle sites, the requirements for effective and accurate fixation of the electrodes are more strict. Thus, referring to fig. 8, in some embodiments, a flexible cord alligator clip 6 is provided within the case 1 for securing the electrode assembly. Specifically, referring to fig. 7 and 8, flexible cord crocodile clip slots 7 for fixing flexible cord crocodile clips are arranged on the box top wall 103, the left side wall 104 and the right side wall 105, the number of the flexible cord crocodile clip slots is selected according to different experimental requirements, and is not limited here, therefore, the flexible cord crocodile clips 6 can provide stable anchor points for stimulating or recording electrodes in the electrode assembly, and the phenomenon that the electrodes are displaced due to accidental displacement of a living animal during an experiment is avoided, so that experimental results are interfered.

The above are only specific embodiments of the present application, but the scope of the present application is not limited thereto, and any changes or substitutions within the technical scope disclosed in the present application should be covered within the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

Claims

1. The shielding box for the electrophysiological experiment comprises a box body, wherein a box door is arranged on one side surface of the box body; it is characterized in that the preparation method is characterized in that,

the box body and the box door are both made of bakelite, and the outer layers of the box body and the box door are wrapped by conductive rubber; electrode lead inlets for electrode leads to pass through are formed in the left side wall and the right side wall of the box body respectively; the rear side wall of the box body is provided with a gas anesthesia catheter inlet for a gas anesthesia catheter to pass through so as to ensure that the experimental animal is in an anesthesia state in the whole process;

an operation platform made of bakelite is arranged in the box body, and conductive rubber is wrapped above the operation platform; the operation platform is provided with an animal head fixing mechanism, and the animal head fixing mechanism comprises a bracket assembly, at least one animal tooth fixing block and at least two animal ear fixing shafts, wherein the at least one animal tooth fixing block and the at least two animal ear fixing shafts are arranged on the bracket assembly;

the bracket component comprises a U-shaped bracket arranged along the horizontal direction, and a first upright post and a second upright post arranged along the vertical direction; the first upright posts are respectively positioned on the corresponding horizontal sections of the U-shaped bracket; the second upright post is positioned on the arc-shaped section of the U-shaped bracket;

the opening of the U-shaped bracket faces the box door;

one ends of the first upright post and the second upright post are connected with the operating platform, and the other ends of the first upright post and the second upright post are connected with the U-shaped bracket in a sliding manner; the U-shaped support can move up and down along the first upright post and the second upright post.

2. The shielding box for electrophysiological experiments of claim 1,

the U-shaped support, the first upright post and the second upright post are square tubes; the U-shaped support is provided with a first through hole which is communicated up and down, the side length of the first through hole is matched with the side lengths of the first upright post and the second upright post, and the U-shaped support is sleeved on the first upright post and the second upright post and can slide up and down along the first upright post and the second upright post;

a plurality of first knobs are connected to the outer side wall of the U-shaped support in a threaded mode, and the threaded sections of the first knobs extend into the U-shaped support and are abutted to the first upright posts or the second upright posts.

3. The electrophysiological experiment shielding box of claim 2, wherein the inner sidewall of the first column has a second through hole, the first end of the animal ear fixing shaft passes through the second through hole and then enters the cavity of the first column, the front sidewall or the rear sidewall of the first column is threaded with a second knob, and the threaded section of the second knob extends into the first column and abuts against the animal ear fixing shaft.

4. The electrophysiological experiment shielding box of claim 2, wherein the second column has a third through hole in its inner sidewall, the first end of the animal tooth fixing block has a slot for engaging with an animal tooth, the second end of the animal tooth fixing block passes through the third through hole and then enters the cavity of the second column, the left or right sidewall of the second column has a third knob in threaded connection, and the threaded section of the third knob extends into the second column and abuts against the animal tooth fixing block.

5. The shielding box of claim 1, wherein the operation platform is slidably connected to the box body and is movable toward or away from the box door.

6. The electrophysiological experiment shielding box of claim 5, wherein two vertical walls of the box body on two sides of the door body are provided with sliding grooves extending in a horizontal direction, and two sides of the operating platform are provided with sliding blocks adapted to the sliding grooves.

7. The electrophysiological experiment shielding box of claim 1, wherein a camera is disposed in the box body, and the camera can capture and record the reaction of the experimental subject in real time.

8. The shielding box for electrophysiological experiments of claim 1, wherein a see-through shielding net is used as a top wall of the box body.

9. The shielding box for electrophysiological experiments of any of claims 1 to 8, wherein a flexible crocodile clip for securing the electrode assembly is provided in the box.