CN113125388A - Fluorescence imaging device and gene sequencer - Google Patents
Fluorescence imaging device and gene sequencer Download PDFInfo
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- CN113125388A CN113125388A CN201911390722.1A CN201911390722A CN113125388A CN 113125388 A CN113125388 A CN 113125388A CN 201911390722 A CN201911390722 A CN 201911390722A CN 113125388 A CN113125388 A CN 113125388A
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- 238000000799 fluorescence microscopy Methods 0.000 title claims abstract description 28
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 17
- 230000003287 optical effect Effects 0.000 claims abstract description 54
- 230000010354 integration Effects 0.000 claims description 11
- 238000003384 imaging method Methods 0.000 abstract description 10
- 238000012163 sequencing technique Methods 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 6
- 238000012632 fluorescent imaging Methods 0.000 description 4
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000003754 machining Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229920000297 Rayon Polymers 0.000 description 1
- 230000001808 coupling effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 230000001105 regulatory effect Effects 0.000 description 1
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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Abstract
A fluorescence imaging device and a gene sequencer are provided, wherein the fluorescence imaging device comprises a base, a camera, a barrel mirror, a first actuator, a second actuator and a third actuator. Because the camera loops through the third base and the second base and is installed on the first base, the barrel mirror is directly installed on the first base, the second base can be adjusted by the first actuator to translate along the optical axis direction relative to the first base, the third base can be adjusted by the second actuator to translate along the integral direction relative to the second base, the camera can be adjusted by the third actuator to rotate along the optical axis relative to the third base, front and back focusing, translation along the integral direction and rotation adjustment along the optical axis can be realized by the camera relative to the barrel mirror, and multiple free adjustments can meet the imaging precision of high-throughput gene sequencing.
Description
Technical Field
The invention relates to the technical field of gene sequencing, in particular to a fluorescence imaging device and a gene sequencer.
Background
The high-throughput gene sequencer is based on the fluorescence microscopic imaging principle, a base is marked by different fluorescence markers, the markers are excited by laser to generate fluorescence, and fluorescence signals are collected by an objective lens and a cylindrical lens and then imaged on a high-sensitivity camera. With the continuous improvement of the TDI camera in indexes such as resolution, line frequency and the like, the TDI camera is more suitable for a gene sequencer with ultrahigh flux compared with an area array camera. In order to obtain a high-quality image of a fluorescence signal, a camera light-sensitive surface needs to be fixed in a focal depth range of a barrel mirror, the focal depth DOF of the barrel mirror is about 100 mu m by taking the barrel mirror with the focal length of 200mm, the F # number of 6.7, the central wavelength of 550nm and the field diameter of 14mm as an example, the tolerance of the complex and the image direction is obtained, the inclination tolerance of the camera light-sensitive surface is about 10', the camera light-sensitive surface can be ensured through the precision machining and assembly of a camera structural part, and in the focal depth direction, a front and back focusing mechanism with the resolution of 10 mu m magnitude is needed to realize the; in order to meet the requirements of subsequent data processing software on images, the camera is required to have the functions of rotation and translation along the integration direction, the rotation resolution of the camera is calculated to be about +/-1 mrad according to edges +/-5 pixel and the radius length 5000pixel, and the translation resolution of the camera along the integration direction is about 10 mu m magnitude according to 2 pixel; in order to simplify the complexity of the adjusting mechanism and improve the reliability of the device, the long edge direction of the camera adopts a windowing mode to replace the translation motion of the long edge. Therefore, the TDI camera needs to have precise adjustment functions such as front and back focusing, translation along the integration direction, rotation along the optical axis, and the like, and the cameras in the prior art cannot achieve such many degrees of freedom adjustment.
Disclosure of Invention
The invention provides a fluorescent imaging device capable of realizing front and back focusing, translation along an integral direction and rotation regulation imaging along an optical axis and a gene sequencer.
According to a first aspect, there is provided in an embodiment a fluorescence imaging apparatus comprising:
the base comprises a first base, a second base and a third base, the second base can be arranged on the first base in an adjustable mode along the optical axis direction, and the third base can be arranged on the second base in an adjustable mode along the integral direction;
the camera is rotatably arranged on the third base along the optical axis;
the cylindrical mirror is arranged on the first base, the lighting end face of the camera is arranged towards the cylindrical mirror, and the cylindrical mirror and the camera are aligned along the optical axis;
the first actuator is connected with the first base and the second base and is used for adjusting the second base to translate along the direction of the optical axis relative to the first base;
the second actuator is connected with the second base and the third base and is used for regulating the third base to translate along the integral direction relative to the second base;
and a third actuator coupled to the third base and the camera, the third actuator for adjusting the camera to rotate relative to the third base along the optical axis.
Furthermore, the lighting end of the camera is provided with an annular groove, the third base is of a flat plate structure perpendicular to the optical axis, an annular bulge is arranged on the surface of the third base facing the camera, and the annular bulge of the third base is rotatably inserted into the annular groove of the camera; the third base is provided with at least two arc-shaped slotted holes positioned on the same circumference, third locking screws are installed in the arc-shaped slotted holes, and the camera is locked on the third base by the third locking screws.
Further, a camera snap ring is installed in the annular groove of the camera, and the annular bulge of the third base is rotatably inserted in the camera snap ring.
Furthermore, the first base is of a flat plate structure parallel to the optical axis, the second base is of an L-shaped plate and comprises a horizontal plate and a vertical plate, the horizontal plate of the second base is connected with the first base, and the vertical plate of the second base is connected with the third base; a horizontal plate of the second base is provided with a first slotted hole along the optical axis direction, a first locking screw is installed in the first slotted hole, and the second base is locked on the first base by the first locking screw; and a second slotted hole along the integral direction is formed in the vertical plate of the second base, a second locking screw is installed in the second slotted hole, and the third base is locked on the second base by the second locking screw.
Further, a guide rail and a guide groove along the optical axis direction are arranged between the horizontal plates of the first base and the second base, and a guide rail and a guide groove along the integral direction are arranged between the vertical plate of the second base and the third base.
Further, the first actuator comprises a first fixed block and a first adjusting screw rod, the first fixed block is arranged in the middle of the side face of the first base, the first adjusting screw rod is connected with the side face of the second base, and the first adjusting screw rod is arranged along the direction of the optical axis; the second actuator comprises a second fixed block and a second adjusting screw rod, the fixed block is arranged in the middle of the side face of the second base, the second adjusting screw rod is connected with the side face of the third base, and the second adjusting screw rod is arranged along the integral direction; the third actuator comprises a third fixing block and a third adjusting screw rod, the third fixing block is installed at the end part of the side face of the third base, the third adjusting screw rod faces the side face of the camera, and the third adjusting screw rod is arranged along the direction perpendicular to the optical axis.
Furthermore, the first actuator also comprises a first adjusting block, the first adjusting block is arranged in the middle of the side surface of the second base, and the first adjusting screw is connected with the first adjusting block; the second actuator further comprises a second adjusting block, the second adjusting block is installed in the middle of the side face of the third base, and the second adjusting screw is connected with the second adjusting block.
Further, the number of the third actuators is four, and the four actuators are symmetrically distributed on two side ends, perpendicular to the first base, of the second base.
Further, the tube mirror is installed on the first base through a flange seat.
According to a second aspect, an embodiment provides a gene sequencer, comprising the fluorescence imaging apparatus described above.
According to the fluorescence imaging device and the gene sequencer of the embodiment, the camera is sequentially arranged on the first base through the third base and the second base, the barrel mirror is directly arranged on the first base, the first actuator can adjust the second base to translate along the optical axis direction relative to the first base, the second actuator can adjust the third base to translate along the integral direction relative to the second base, and the third actuator can adjust the camera to rotate along the optical axis relative to the third base, so that the camera can realize front and back focusing, translation along the integral direction and rotation adjustment along the optical axis relative to the barrel mirror, and multi-freedom adjustment can meet the imaging precision of high-throughput gene sequencing.
Drawings
FIG. 1 is a schematic diagram of a fluorescence imaging apparatus according to an embodiment;
FIG. 2 is a schematic diagram of a fluorescence imaging apparatus according to an embodiment;
FIG. 3 is a schematic diagram of a first base according to an embodiment;
FIG. 4 is a schematic diagram of a second base according to an embodiment;
FIG. 5 is a schematic diagram of a third exemplary embodiment of a base;
FIG. 6 is a schematic diagram of a third exemplary embodiment of a base;
FIG. 7 is an axial cross-sectional view of a fluorescence imaging device in one embodiment.
Detailed Description
The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.
Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).
In one embodiment, a fluorescence imaging apparatus is provided, which is based on a camera for imaging, and the camera can perform focusing back and forth, translation along an integration direction, and rotation along an optical axis for adjusting imaging. The camera in this embodiment may be a TDI camera or other cameras for fluorescence imaging.
As shown in fig. 1 and 2, the fluorescence imaging apparatus of the present embodiment includes a base 10, a camera 20, a barrel mirror 30, a first actuator 40, a second actuator 50, and a third actuator 60, wherein the first actuator 40, the second actuator 50, and the third actuator 60 are used to drive the camera 20 to adjust to a precise position.
The base 10 includes a first base 11, a second base 12 and a third base 13, and the first base 11 is a flat plate structure. The barrel mirror 30 has a plurality of lenses, the barrel mirror 30 is used for emitting imaging light to the camera 20, the barrel mirror 30 is mounted on the right end of the first base 11 through the flange seat 14, and the optical axis of the barrel mirror 30 is parallel to the first base 11.
As shown in fig. 1, 3 and 4, the second base 12 is an L-shaped plate, the second base 12 includes a horizontal plate and a vertical plate, the horizontal plate of the second base 12 is installed at the left end of the first base 11, the upper surface of the first base 11 is provided with a guide rail 11a (also can be a flat key installed in the guide groove) along the optical axis direction, the lower surface of the horizontal plate of the second base 12 is provided with a corresponding guide groove 12a, the guide groove 12a of the second base 12 is clamped on the guide rail 11a of the first base 11, so that the second base 12 can translate along the optical axis direction relative to the first base 11, the guide rail 11a and the guide groove 12a are provided to limit, the second base 12 is prevented from translating in other directions, and the adjustment accuracy and the structural stability are improved. The upper surface of the first base 11 is provided with a guide groove, and the lower surface of the horizontal plate of the second base 12 is provided with a guide rail or a slide block, which can also play a role in axial limiting. The horizontal plate of the second base 12 is further provided with a plurality of first slots 12b along the optical axis direction, each first slot 12b is internally provided with a first locking screw 71, the first base 11 is provided with a corresponding threaded hole, and the first locking screw 71 locks the second base 12 on the first base 11.
As shown in fig. 1, 2, 5 and 6, the third base 13 is a flat plate structure, and the third base 13 is mounted on a surface of the vertical plate of the second base 12 facing away from the barrel mirror 30. The vertical plate of the second base 12 is provided with a guide groove 12c along the integral direction (the horizontal direction perpendicular to the optical axis) on the surface facing the third base 13, the third base 13 is provided with a corresponding guide rail 13a (which can also be set as a guide block) on the surface facing the second base 12, and the guide rail 13a of the third base 13 is clamped in the guide groove 12c of the second base 12, so that the third base 13 can translate along the integral direction relative to the second base 13, the guide rail 13a and the guide groove 12c also play a role in limiting, and the adjustment precision and the structural stability are improved. The vertical plate of the second base 12 is provided with a guide rail or a guide block, and the third base 13 is provided with a guide groove, which can also play a role of limiting along the integral direction. The second base 12 is provided with a plurality of second slots 12d along the integration direction, a second locking screw 72 is installed in each second slot 12d, the third base 13 is provided with a corresponding screw hole, and the second locking screw 72 locks the third base 13 on the second base 12.
As shown in fig. 2, 6 and 7, the vertical plate of the second base 12 and the middle portion of the third base 13 have aligned through holes for avoiding imaging light. The third base 13 is provided with an annular protrusion 13b on the surface of the vertical plate facing away from the second base 12, and the annular protrusion 13b surrounds the edge of the through hole. The daylighting end of camera 20 has annular groove 21, installs camera snap ring 22 in the annular groove 21, and camera snap ring 22's surface external screw thread, internal surface are the smooth surface, have the internal thread in the annular groove 21, and camera snap ring 22 is installed in the annular groove 21 through threaded connection's mode, and camera snap ring 22 also can be fixed in the annular groove 21 through the mode of viscose or joint. Annular protrusion 13b cartridge of third base 13 is in camera snap ring 22, and annular protrusion 13b and camera snap ring 22 of third base 13 form swivelling joint to camera 20 can be rotatory along the optical axis relative to third base 13, and camera snap ring 22 plays transitional coupling's effect, has avoided the direct rotatory wearing and tearing that cause with the contact of third base 13 of camera 20, and camera snap ring 22 can also be changed after wearing and tearing, also can improve rotatory machining precision. The third base 13 is further provided with 4 arc-shaped slotted holes 13c located on one circumference, the 4 arc-shaped slotted holes 13c are located at four corners of the third base 13, the surface of the camera 20 facing the third base 13 is provided with corresponding threaded holes, a third locking screw 73 is installed in each arc-shaped slotted hole 13c, and the camera 20 is locked on the third base 13 by the third locking screws 73. The second base 12 is provided with 4 corresponding arc-shaped slots 12e, and the arc-shaped slots 12e on the second base 12 play a role of avoiding, so that the third locking screw 73 can pass through the second base 12 to connect the third base 13 and the camera 20. The preferred arc-shaped slot 13c is a countersunk slot so that the head of the third locking screw 73 can be hidden in the arc-shaped slot 13c of the third base 13.
In other embodiments, the annular protrusion 13b of the third base 13 is directly inserted into the annular groove 21 of the camera 20, although it may cause some wear, and may also serve as a rotational coupling. The arc-shaped slotted holes 13c can also be arranged into two or three evenly positioned on a circle, and can also play a role of rotation locking.
As shown in fig. 1, in the present embodiment, the first actuator 40 includes a first fixed block 41 and a first adjusting screw 42, the first fixed block 41 is installed in the middle of the left end side surface of the first base 11 by screws, a threaded hole is formed in the first fixed block 41, the first adjusting screw 42 is installed in the threaded hole of the first fixed block 41, the first adjusting screw 42 is disposed along the optical axis, the first adjusting screw 42 has a rotating end and a driving end, the rotating end of the first adjusting screw 42 is used for adjusting translation, and the driving end of the first adjusting screw 42 is connected to the left end surface of the second base 12. Because the thickness of the second base 12 is relatively thin, the first actuator 40 further includes a first adjusting block 43, the first adjusting block 43 is mounted at the middle of the left side surface of the second base 12 through a screw, the driving end of the first adjusting screw 42 is connected with the first adjusting block 43, and the first adjusting block 43 plays a role of transitional connection.
The second actuator 50 includes a second fixed block 51 and a second adjusting screw 52, the second fixed block 51 is mounted in the middle of the side of the vertical plate of the second base 12 through a screw, a threaded hole is formed in the second fixed block 51, the second adjusting screw 52 is mounted in the threaded hole of the second fixed block 51, the second adjusting screw 52 is arranged along the integral direction, the second adjusting screw 52 has a rotating end and a driving end, the rotating end of the second adjusting screw 52 is used for adjusting translation, and the driving end of the second adjusting screw 52 is connected with the side of the third base 13. Because the thickness of the third base 13 is relatively thin, the second actuator 50 further includes a second adjusting block 53, the second adjusting block 53 is mounted in the middle of the side surface of the third base 13 through a screw, the driving end of the second adjusting screw 52 is connected with the second adjusting block 53, and the second adjusting block 53 plays a role in transitional connection.
The third actuators 60 have four, and the four third actuators 60 are distributed on the upper and lower ends of the two vertical sides of the third base 13. The third actuator 60 includes a third fixed block 61 and a third adjusting screw 62, the third fixed block 61 is fixed on the third base 13 by screws, a threaded hole is formed in the third fixed block 61, the third adjusting screw 62 is installed in the threaded hole of the third fixed block 61, the third adjusting screw 62 is arranged along the integral direction, the third adjusting screw 62 has a rotating end and a driving end, the rotating end of the third adjusting screw 62 is used for adjusting rotation, and the driving end of the third adjusting screw 62 is arranged toward the side of the camera 20. There is a gap between the third adjusting screws 62 of the four third actuators 60 and the camera 20, and the four third actuators 60 are divided into two groups, each group of the third actuators 60 includes two third actuators 60 on the diagonal, and the two groups of the third actuators 60 are used for driving the camera to rotate in different directions along the optical axis.
In the fluorescence imaging apparatus of the present embodiment, the camera 20 can perform front and back focusing, translation along the integration direction, and rotation along the optical axis for imaging with respect to the barrel mirror 30, and the specific adjustment manner is as follows:
front and back focusing adjustment: all the first locking screws 71 are loosened, then the rotating end of the first adjusting screw 42 is driven, the first adjusting screw 42 drives the second base 12 to move along the optical axis relative to the first base 11, that is, the camera 20 is driven to move along the optical axis relative to the barrel mirror 30, and after the camera 20 moves in place along the optical axis direction, all the first locking screws 71 are locked, the position of the camera 20 along the optical axis direction is fixed, and focusing is completed.
Adjustment of translation in the integration direction: all the second locking screws 72 are loosened, then the rotating end of the second adjusting screw 52 is driven, the second adjusting screw 52 drives the third base 13 to move along the integrating direction relative to the second base 12, that is, the camera 20 is driven to move along the integrating direction relative to the barrel mirror 30, and after the camera 20 moves in place along the integrating direction, all the second locking screws 72 are locked, the position of the camera 20 along the integrating direction is fixed, and the translation along the integrating direction is completed.
And (3) rotationally adjusting along the optical axis: all the third locking screws 73 are loosened, the rotating ends of the third adjusting screws 62 of the two groups of third actuators 60 are driven, the driving ends of the third adjusting screws 62 of the two groups of third actuators 60 abut against the side face of the camera 20 respectively, the camera 20 is driven to rotate along the optical axis relative to the third base 13, namely, the camera 20 is driven to rotate along the optical axis relative to the barrel mirror 30, after the camera 20 rotates to a position along the optical axis, all the third locking screws 73 are locked finally, the rotating position of the camera 20 along the optical axis is fixed, and the rotating ends of the third adjusting screws 62 of the two groups of third actuators 60 are rotated away from the side face of the camera 20, so that the optical axis rotation adjustment is completed.
In the fluorescence imaging apparatus provided in this embodiment, because the camera 20 is sequentially installed on the first base 11 through the third base 13 and the second base 12, the barrel mirror 30 is directly installed on the first base 11, the first actuator 40 can adjust the second base 12 to translate along the optical axis direction relative to the first base 11, the second actuator 50 can adjust the third base 13 to translate along the integration direction relative to the second base 12, and the third actuator 60 can adjust the camera 20 to rotate along the optical axis relative to the third base 13, so that the camera 20 can realize front-back focusing, translation along the integration direction, and rotation adjustment along the optical axis relative to the barrel mirror 30, and multiple degrees of freedom can meet the imaging accuracy of high-throughput gene sequencing. Moreover, the fluorescence imaging device has the advantages of compact structure, stable integral structure, strong precision stability and small occupied space.
The gene sequencer comprises the fluorescent imaging device in the embodiment, and the fluorescent imaging device can realize front and back focusing, translation along the integral direction and rotation along the optical axis for adjustment and imaging, so that the gene sequencer can obtain more accurate fluorescent imaging, and the gene detection precision is improved.
The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.
Claims (10)
1. A fluorescence imaging apparatus, comprising:
a base including a first base, a second base mounted on the first base to be adjustable in an optical axis direction, and a third base mounted on the second base to be adjustable in an integration direction;
a camera rotatably mounted on the third base along an optical axis;
a barrel mirror mounted on the first base, a light-collecting end of the camera disposed toward the barrel mirror, and the barrel mirror and the camera aligned along an optical axis;
a first actuator coupled to the first and second bases, the first actuator for adjusting the second base to translate relative to the first base along the optical axis;
a second actuator coupled to the second base and a third base, the second actuator for adjusting the third base to translate relative to the second base along an integration direction;
and a third actuator coupled to the third base and the camera, the third actuator for adjusting the camera to rotate relative to the third base along the optical axis.
2. The fluorescence imaging device according to claim 1, wherein the light collecting end of the camera has an annular groove, the third base is a flat plate structure perpendicular to the optical axis, an annular protrusion is disposed on a surface of the third base facing the camera, and the annular protrusion of the third base is rotatably inserted into the annular groove of the camera; the camera is characterized in that the third base is provided with at least two arc-shaped slotted holes positioned on the same circumference, third locking screws are installed in the arc-shaped slotted holes, and the third locking screws lock the camera on the third base.
3. The fluorescence imaging device of claim 2, wherein said camera annular groove has a camera snap ring mounted therein, and said third base annular protrusion is rotatably inserted into said camera snap ring.
4. The fluorescence imaging apparatus according to claim 1, wherein said first base is a flat plate structure parallel to an optical axis, said second base is an L-shaped plate, said second base includes a horizontal plate and a vertical plate, said horizontal plate of said second base is connected to said first base, said vertical plate of said second base is connected to said third base; a horizontal plate of the second base is provided with a first slotted hole along the optical axis direction, a first locking screw is installed in the first slotted hole, and the second base is locked on the first base by the first locking screw; and a second slotted hole along the integral direction is formed in the vertical plate of the second base, a second locking screw is installed in the second slotted hole, and the third base is locked on the second base by the second locking screw.
5. The fluorescence imaging apparatus according to claim 4, wherein a guide rail and a guide groove in an optical axis direction are provided between the first base and the horizontal plate of the second base, and a guide rail and a guide groove in an integrating direction are provided between the vertical plate of the second base and the third base.
6. The fluorescence imaging apparatus according to claim 1, wherein said first actuator includes a first fixed block and a first adjusting screw, said first fixed block being mounted in a middle portion of a side surface of said first base, said first adjusting screw being connected to a side surface of said second base, said first adjusting screw being disposed in an optical axis direction; the second actuator comprises a second fixed block and a second adjusting screw rod, the fixed block is arranged in the middle of the side face of the second base, the second adjusting screw rod is connected with the side face of the third base, and the second adjusting screw rod is arranged along the integral direction; the third actuator comprises a third fixed block and a third adjusting screw, the third fixed block is installed at the end part of the side face of the third base, the third adjusting screw faces the side face of the camera, and the third adjusting screw is arranged along the direction perpendicular to the optical axis.
7. The fluorescence imaging device of claim 6, wherein said first actuator further comprises a first adjustment block, said first adjustment block mounted in a lateral middle of said second base, said first adjustment screw connected to said first adjustment block; the second actuator further comprises a second adjusting block, the second adjusting block is installed in the middle of the side face of the third base, and the second adjusting screw is connected with the second adjusting block.
8. The fluorescence imaging apparatus according to claim 6, wherein said third actuator has four actuators, and four of said actuators are symmetrically disposed on both side ends of said second base perpendicular to said first base.
9. The fluorescence imaging device of claim 1, wherein said barrel mirror is mounted on said first base by a flange mount.
10. A gene sequencer comprising the fluorescence imaging apparatus according to any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911390722.1A CN113125388A (en) | 2019-12-30 | 2019-12-30 | Fluorescence imaging device and gene sequencer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911390722.1A CN113125388A (en) | 2019-12-30 | 2019-12-30 | Fluorescence imaging device and gene sequencer |
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| CN113125388A true CN113125388A (en) | 2021-07-16 |
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| CN201911390722.1A Pending CN113125388A (en) | 2019-12-30 | 2019-12-30 | Fluorescence imaging device and gene sequencer |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB195818A (en) * | 1922-02-07 | 1923-04-12 | Ernest Hall Craggs | Improvements in and relating to microscopes and devices for microphotography |
| WO2001040747A1 (en) * | 1999-12-01 | 2001-06-07 | Ryer Damond V | Spectral instrument using multiple non-interfering optical beam paths and elements for use therewith |
| US20060115260A1 (en) * | 2004-12-01 | 2006-06-01 | Pentax Corporation | Imaging device having an angle adjusting device for an image sensor |
| CN203191616U (en) * | 2013-01-17 | 2013-09-11 | 深圳迈瑞生物医疗电子股份有限公司 | Optics adjusting frame |
| JP2014052420A (en) * | 2012-09-05 | 2014-03-20 | Hoya Corp | Optical means support structure |
| CN104793334A (en) * | 2015-04-02 | 2015-07-22 | 同济大学 | Cascading coarse-fine data coupling optical scanning device |
| CN105511122A (en) * | 2015-11-25 | 2016-04-20 | 武汉精测电子技术股份有限公司 | Automatic positioning focusing detection apparatus based on online machine visual inspection |
| CN105779267A (en) * | 2014-12-16 | 2016-07-20 | 深圳华大基因研究院 | Optical system used for gene sequencer |
| US20180180984A1 (en) * | 2014-06-26 | 2018-06-28 | Yeon Systems Co., Ltd. | Monocular stereoscopic camera |
| CN209604778U (en) * | 2018-11-30 | 2019-11-08 | 深圳华大生命科学研究院 | Multiple degrees of freedom regulating device and the imaging device for using the multiple degrees of freedom regulating device |
-
2019
- 2019-12-30 CN CN201911390722.1A patent/CN113125388A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB195818A (en) * | 1922-02-07 | 1923-04-12 | Ernest Hall Craggs | Improvements in and relating to microscopes and devices for microphotography |
| WO2001040747A1 (en) * | 1999-12-01 | 2001-06-07 | Ryer Damond V | Spectral instrument using multiple non-interfering optical beam paths and elements for use therewith |
| US20060115260A1 (en) * | 2004-12-01 | 2006-06-01 | Pentax Corporation | Imaging device having an angle adjusting device for an image sensor |
| JP2014052420A (en) * | 2012-09-05 | 2014-03-20 | Hoya Corp | Optical means support structure |
| CN203191616U (en) * | 2013-01-17 | 2013-09-11 | 深圳迈瑞生物医疗电子股份有限公司 | Optics adjusting frame |
| US20180180984A1 (en) * | 2014-06-26 | 2018-06-28 | Yeon Systems Co., Ltd. | Monocular stereoscopic camera |
| CN105779267A (en) * | 2014-12-16 | 2016-07-20 | 深圳华大基因研究院 | Optical system used for gene sequencer |
| CN104793334A (en) * | 2015-04-02 | 2015-07-22 | 同济大学 | Cascading coarse-fine data coupling optical scanning device |
| CN105511122A (en) * | 2015-11-25 | 2016-04-20 | 武汉精测电子技术股份有限公司 | Automatic positioning focusing detection apparatus based on online machine visual inspection |
| CN209604778U (en) * | 2018-11-30 | 2019-11-08 | 深圳华大生命科学研究院 | Multiple degrees of freedom regulating device and the imaging device for using the multiple degrees of freedom regulating device |
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