CN113122547B - CsMYB110基因在调控类胡萝卜素合成中的应用 - Google Patents
CsMYB110基因在调控类胡萝卜素合成中的应用 Download PDFInfo
- Publication number
- CN113122547B CN113122547B CN202110425738.2A CN202110425738A CN113122547B CN 113122547 B CN113122547 B CN 113122547B CN 202110425738 A CN202110425738 A CN 202110425738A CN 113122547 B CN113122547 B CN 113122547B
- Authority
- CN
- China
- Prior art keywords
- csmyb110
- gene
- carotenoid
- tea
- synthesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000021466 carotenoid Nutrition 0.000 title claims abstract description 61
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 61
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 48
- 150000001747 carotenoids Chemical class 0.000 title claims abstract description 45
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 35
- 230000033228 biological regulation Effects 0.000 title claims abstract description 15
- 239000002773 nucleotide Substances 0.000 claims abstract description 4
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 4
- 244000269722 Thea sinensis Species 0.000 claims description 47
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims description 28
- 240000003553 Leptospermum scoparium Species 0.000 claims description 28
- 235000015459 Lycium barbarum Nutrition 0.000 claims description 28
- 101000662893 Arabidopsis thaliana Telomere repeat-binding factor 1 Proteins 0.000 claims description 3
- 101000662890 Arabidopsis thaliana Telomere repeat-binding factor 2 Proteins 0.000 claims description 3
- 101000662891 Arabidopsis thaliana Telomere repeat-binding factor 3 Proteins 0.000 claims description 3
- 101000662896 Arabidopsis thaliana Telomere repeat-binding factor 4 Proteins 0.000 claims description 3
- 101000662897 Arabidopsis thaliana Telomere repeat-binding factor 5 Proteins 0.000 claims description 3
- 230000014509 gene expression Effects 0.000 abstract description 25
- 241000196324 Embryophyta Species 0.000 abstract description 10
- 238000009825 accumulation Methods 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 238000010367 cloning Methods 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 238000010353 genetic engineering Methods 0.000 abstract description 3
- 230000007246 mechanism Effects 0.000 abstract description 3
- 241001122767 Theaceae Species 0.000 abstract 4
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 235000013616 tea Nutrition 0.000 description 36
- -1 carotenoid compound Chemical class 0.000 description 18
- 241000208125 Nicotiana Species 0.000 description 17
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 17
- 238000004458 analytical method Methods 0.000 description 13
- 238000011144 upstream manufacturing Methods 0.000 description 12
- 235000006468 Thea sinensis Nutrition 0.000 description 11
- 108091034117 Oligonucleotide Proteins 0.000 description 10
- 210000001519 tissue Anatomy 0.000 description 10
- 230000000692 anti-sense effect Effects 0.000 description 9
- 230000005764 inhibitory process Effects 0.000 description 8
- 239000000049 pigment Substances 0.000 description 8
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 7
- 235000013734 beta-carotene Nutrition 0.000 description 7
- 239000011648 beta-carotene Substances 0.000 description 7
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 7
- 229960002747 betacarotene Drugs 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000013598 vector Substances 0.000 description 7
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 239000013612 plasmid Substances 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 description 6
- 108091023040 Transcription factor Proteins 0.000 description 5
- 102000040945 Transcription factor Human genes 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000001656 lutein Substances 0.000 description 5
- 235000012680 lutein Nutrition 0.000 description 5
- 229960005375 lutein Drugs 0.000 description 5
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 description 5
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 230000002018 overexpression Effects 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 101710173432 Phytoene synthase Proteins 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000010839 reverse transcription Methods 0.000 description 4
- 230000004960 subcellular localization Effects 0.000 description 4
- 230000009261 transgenic effect Effects 0.000 description 4
- 230000001052 transient effect Effects 0.000 description 4
- OINNEUNVOZHBOX-QIRCYJPOSA-K 2-trans,6-trans,10-trans-geranylgeranyl diphosphate(3-) Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CC\C(C)=C\COP([O-])(=O)OP([O-])([O-])=O OINNEUNVOZHBOX-QIRCYJPOSA-K 0.000 description 3
- OINNEUNVOZHBOX-XBQSVVNOSA-N Geranylgeranyl diphosphate Natural products [P@](=O)(OP(=O)(O)O)(OC/C=C(\CC/C=C(\CC/C=C(\CC/C=C(\C)/C)/C)/C)/C)O OINNEUNVOZHBOX-XBQSVVNOSA-N 0.000 description 3
- 102100034343 Integrase Human genes 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- 230000009418 agronomic effect Effects 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000010195 expression analysis Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- NUHSROFQTUXZQQ-UHFFFAOYSA-N isopentenyl diphosphate Chemical compound CC(=C)CCO[P@](O)(=O)OP(O)(O)=O NUHSROFQTUXZQQ-UHFFFAOYSA-N 0.000 description 3
- 239000002207 metabolite Substances 0.000 description 3
- 230000037361 pathway Effects 0.000 description 3
- CBIDRCWHNCKSTO-UHFFFAOYSA-N prenyl diphosphate Chemical compound CC(C)=CCO[P@](O)(=O)OP(O)(O)=O CBIDRCWHNCKSTO-UHFFFAOYSA-N 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- YVLPJIGOMTXXLP-UHFFFAOYSA-N 15-cis-phytoene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CC=CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C YVLPJIGOMTXXLP-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 101150019514 CRTISO gene Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- FADYJNXDPBKVCA-UHFFFAOYSA-N L-Phenylalanyl-L-lysin Natural products NCCCCC(C(O)=O)NC(=O)C(N)CC1=CC=CC=C1 FADYJNXDPBKVCA-UHFFFAOYSA-N 0.000 description 2
- SBANPBVRHYIMRR-UHFFFAOYSA-N Leu-Ser-Pro Natural products CC(C)CC(N)C(=O)NC(CO)C(=O)N1CCCC1C(O)=O SBANPBVRHYIMRR-UHFFFAOYSA-N 0.000 description 2
- 238000002123 RNA extraction Methods 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- TZVUSFMQWPWHON-NHCYSSNCSA-N Val-Asp-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](C(C)C)N TZVUSFMQWPWHON-NHCYSSNCSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 230000019771 cognition Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000009833 condensation Methods 0.000 description 2
- 230000005494 condensation Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000008303 genetic mechanism Effects 0.000 description 2
- 108010037850 glycylvaline Proteins 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 230000037353 metabolic pathway Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000024053 secondary metabolic process Effects 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 150000003505 terpenes Chemical class 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- PSQYTAPXSHCGMF-BQYQJAHWSA-N β-ionone Chemical compound CC(=O)\C=C\C1=C(C)CCCC1(C)C PSQYTAPXSHCGMF-BQYQJAHWSA-N 0.000 description 2
- SFEOKXHPFMOVRM-UHFFFAOYSA-N (+)-(S)-gamma-ionone Natural products CC(=O)C=CC1C(=C)CCCC1(C)C SFEOKXHPFMOVRM-UHFFFAOYSA-N 0.000 description 1
- JKQXZKUSFCKOGQ-JLGXGRJMSA-N (3R,3'R)-beta,beta-carotene-3,3'-diol Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C[C@@H](O)CC1(C)C JKQXZKUSFCKOGQ-JLGXGRJMSA-N 0.000 description 1
- LXJXRIRHZLFYRP-VKHMYHEASA-L (R)-2-Hydroxy-3-(phosphonooxy)-propanal Natural products O=C[C@H](O)COP([O-])([O-])=O LXJXRIRHZLFYRP-VKHMYHEASA-L 0.000 description 1
- KJTLQQUUPVSXIM-ZCFIWIBFSA-M (R)-mevalonate Chemical compound OCC[C@](O)(C)CC([O-])=O KJTLQQUUPVSXIM-ZCFIWIBFSA-M 0.000 description 1
- YVLPJIGOMTXXLP-UUKUAVTLSA-N 15,15'-cis-Phytoene Natural products C(=C\C=C/C=C(\CC/C=C(\CC/C=C(\CC/C=C(\C)/C)/C)/C)/C)(\CC/C=C(\CC/C=C(\CC/C=C(\C)/C)/C)/C)/C YVLPJIGOMTXXLP-UUKUAVTLSA-N 0.000 description 1
- YVLPJIGOMTXXLP-BAHRDPFUSA-N 15Z-phytoene Natural products CC(=CCCC(=CCCC(=CCCC(=CC=C/C=C(C)/CCC=C(/C)CCC=C(/C)CCC=C(C)C)C)C)C)C YVLPJIGOMTXXLP-BAHRDPFUSA-N 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- GFBLJMHGHAXGNY-ZLUOBGJFSA-N Ala-Asn-Asp Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O GFBLJMHGHAXGNY-ZLUOBGJFSA-N 0.000 description 1
- STACJSVFHSEZJV-GHCJXIJMSA-N Ala-Asn-Ile Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O STACJSVFHSEZJV-GHCJXIJMSA-N 0.000 description 1
- DVJSJDDYCYSMFR-ZKWXMUAHSA-N Ala-Ile-Gly Chemical compound [H]N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(O)=O DVJSJDDYCYSMFR-ZKWXMUAHSA-N 0.000 description 1
- ZRNWJUAQKFUUKV-SRVKXCTJSA-N Arg-Met-Met Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCSC)C(O)=O ZRNWJUAQKFUUKV-SRVKXCTJSA-N 0.000 description 1
- WKPXXXUSUHAXDE-SRVKXCTJSA-N Arg-Pro-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C(N)N)C(O)=O WKPXXXUSUHAXDE-SRVKXCTJSA-N 0.000 description 1
- HNJNAMGZQZPSRE-GUBZILKMSA-N Arg-Pro-Asn Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(O)=O HNJNAMGZQZPSRE-GUBZILKMSA-N 0.000 description 1
- YHZQOSXDTFRZKU-WDSOQIARSA-N Arg-Trp-Leu Chemical compound C1=CC=C2C(C[C@@H](C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)[C@@H](N)CCCN=C(N)N)=CNC2=C1 YHZQOSXDTFRZKU-WDSOQIARSA-N 0.000 description 1
- XMGVWQWEWWULNS-BPUTZDHNSA-N Arg-Trp-Ser Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N XMGVWQWEWWULNS-BPUTZDHNSA-N 0.000 description 1
- BZMWJLLUAKSIMH-FXQIFTODSA-N Asn-Glu-Glu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O BZMWJLLUAKSIMH-FXQIFTODSA-N 0.000 description 1
- JEEFEQCRXKPQHC-KKUMJFAQSA-N Asn-Leu-Phe Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O JEEFEQCRXKPQHC-KKUMJFAQSA-N 0.000 description 1
- RLHANKIRBONJBK-IHRRRGAJSA-N Asn-Met-Phe Chemical compound CSCC[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)NC(=O)[C@H](CC(=O)N)N RLHANKIRBONJBK-IHRRRGAJSA-N 0.000 description 1
- DATSKXOXPUAOLK-KKUMJFAQSA-N Asn-Tyr-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(O)=O DATSKXOXPUAOLK-KKUMJFAQSA-N 0.000 description 1
- SNDBKTFJWVEVPO-WHFBIAKZSA-N Asp-Gly-Ser Chemical compound [H]N[C@@H](CC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(O)=O SNDBKTFJWVEVPO-WHFBIAKZSA-N 0.000 description 1
- WNGZKSVJFDZICU-XIRDDKMYSA-N Asp-Leu-Trp Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)NC(=O)[C@H](CC(=O)O)N WNGZKSVJFDZICU-XIRDDKMYSA-N 0.000 description 1
- FQHBAQLBIXLWAG-DCAQKATOSA-N Asp-Lys-Met Chemical compound CSCC[C@@H](C(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(=O)O)N FQHBAQLBIXLWAG-DCAQKATOSA-N 0.000 description 1
- MGSVBZIBCCKGCY-ZLUOBGJFSA-N Asp-Ser-Ser Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O MGSVBZIBCCKGCY-ZLUOBGJFSA-N 0.000 description 1
- MBPKYKSYUAPLMY-DCAQKATOSA-N Cys-Arg-Leu Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(O)=O MBPKYKSYUAPLMY-DCAQKATOSA-N 0.000 description 1
- NLCZGISONIGRQP-DCAQKATOSA-N Cys-Arg-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CS)N NLCZGISONIGRQP-DCAQKATOSA-N 0.000 description 1
- LXJXRIRHZLFYRP-VKHMYHEASA-N D-glyceraldehyde 3-phosphate Chemical compound O=C[C@H](O)COP(O)(O)=O LXJXRIRHZLFYRP-VKHMYHEASA-N 0.000 description 1
- KJTLQQUUPVSXIM-UHFFFAOYSA-N DL-mevalonic acid Natural products OCCC(O)(C)CC(O)=O KJTLQQUUPVSXIM-UHFFFAOYSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- GVVPGTZRZFNKDS-YFHOEESVSA-N Geranyl diphosphate Natural products CC(C)=CCC\C(C)=C/COP(O)(=O)OP(O)(O)=O GVVPGTZRZFNKDS-YFHOEESVSA-N 0.000 description 1
- GNMQDOGFWYWPNM-LAEOZQHASA-N Gln-Gly-Ile Chemical compound CC[C@H](C)[C@H](NC(=O)CNC(=O)[C@@H](N)CCC(N)=O)C(O)=O GNMQDOGFWYWPNM-LAEOZQHASA-N 0.000 description 1
- JRHPEMVLTRADLJ-AVGNSLFASA-N Gln-Lys-Lys Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)N)N JRHPEMVLTRADLJ-AVGNSLFASA-N 0.000 description 1
- NTBDVNJIWCKURJ-ACZMJKKPSA-N Glu-Asp-Asn Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O NTBDVNJIWCKURJ-ACZMJKKPSA-N 0.000 description 1
- CXRWMMRLEMVSEH-PEFMBERDSA-N Glu-Ile-Asn Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(N)=O)C(O)=O CXRWMMRLEMVSEH-PEFMBERDSA-N 0.000 description 1
- ZSWGJYOZWBHROQ-RWRJDSDZSA-N Glu-Ile-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(O)=O ZSWGJYOZWBHROQ-RWRJDSDZSA-N 0.000 description 1
- MRWYPDWDZSLWJM-ACZMJKKPSA-N Glu-Ser-Asp Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O MRWYPDWDZSLWJM-ACZMJKKPSA-N 0.000 description 1
- TWYSSILQABLLME-HJGDQZAQSA-N Glu-Thr-Arg Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O TWYSSILQABLLME-HJGDQZAQSA-N 0.000 description 1
- JVYNYWXHZWVJEF-NUMRIWBASA-N Glu-Thr-Asn Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CCC(=O)O)N)O JVYNYWXHZWVJEF-NUMRIWBASA-N 0.000 description 1
- OVSKVOOUFAKODB-UWVGGRQHSA-N Gly-Arg-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CCCN=C(N)N OVSKVOOUFAKODB-UWVGGRQHSA-N 0.000 description 1
- XTQFHTHIAKKCTM-YFKPBYRVSA-N Gly-Glu-Gly Chemical compound NCC(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O XTQFHTHIAKKCTM-YFKPBYRVSA-N 0.000 description 1
- HAXARWKYFIIHKD-ZKWXMUAHSA-N Gly-Ile-Ser Chemical compound NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(O)=O HAXARWKYFIIHKD-ZKWXMUAHSA-N 0.000 description 1
- GJHWILMUOANXTG-WPRPVWTQSA-N Gly-Val-Arg Chemical compound [H]NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O GJHWILMUOANXTG-WPRPVWTQSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 101001094048 Homo sapiens Pendrin Proteins 0.000 description 1
- DMHGKBGOUAJRHU-RVMXOQNASA-N Ile-Arg-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N1CCC[C@@H]1C(=O)O)N DMHGKBGOUAJRHU-RVMXOQNASA-N 0.000 description 1
- DMHGKBGOUAJRHU-UHFFFAOYSA-N Ile-Arg-Pro Natural products CCC(C)C(N)C(=O)NC(CCCN=C(N)N)C(=O)N1CCCC1C(O)=O DMHGKBGOUAJRHU-UHFFFAOYSA-N 0.000 description 1
- WUKLZPHVWAMZQV-UKJIMTQDSA-N Ile-Glu-Val Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C(C)C)C(=O)O)N WUKLZPHVWAMZQV-UKJIMTQDSA-N 0.000 description 1
- NZGTYCMLUGYMCV-XUXIUFHCSA-N Ile-Lys-Arg Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)N NZGTYCMLUGYMCV-XUXIUFHCSA-N 0.000 description 1
- WYUHAXJAMDTOAU-IAVJCBSLSA-N Ile-Phe-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)O)N WYUHAXJAMDTOAU-IAVJCBSLSA-N 0.000 description 1
- BATWGBRIZANGPN-ZPFDUUQYSA-N Ile-Pro-Gln Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)N)C(=O)O)N BATWGBRIZANGPN-ZPFDUUQYSA-N 0.000 description 1
- IPFKIGNDTUOFAF-CYDGBPFRSA-N Ile-Val-Arg Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CCCN=C(N)N IPFKIGNDTUOFAF-CYDGBPFRSA-N 0.000 description 1
- IPMYMEWFZKHGAX-UHFFFAOYSA-N Isotheaflavin Natural products OC1CC2=C(O)C=C(O)C=C2OC1C(C1=C2)=CC(O)=C(O)C1=C(O)C(=O)C=C2C1C(O)CC2=C(O)C=C(O)C=C2O1 IPMYMEWFZKHGAX-UHFFFAOYSA-N 0.000 description 1
- IBMVEYRWAWIOTN-UHFFFAOYSA-N L-Leucyl-L-Arginyl-L-Proline Natural products CC(C)CC(N)C(=O)NC(CCCN=C(N)N)C(=O)N1CCCC1C(O)=O IBMVEYRWAWIOTN-UHFFFAOYSA-N 0.000 description 1
- 239000012880 LB liquid culture medium Substances 0.000 description 1
- 201000010743 Lambert-Eaton myasthenic syndrome Diseases 0.000 description 1
- 241000880493 Leptailurus serval Species 0.000 description 1
- IGUOAYLTQJLPPD-DCAQKATOSA-N Leu-Asn-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N IGUOAYLTQJLPPD-DCAQKATOSA-N 0.000 description 1
- FMEICTQWUKNAGC-YUMQZZPRSA-N Leu-Gly-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(O)=O FMEICTQWUKNAGC-YUMQZZPRSA-N 0.000 description 1
- KVOFSTUWVSQMDK-KKUMJFAQSA-N Leu-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(C)C)CC1=CN=CN1 KVOFSTUWVSQMDK-KKUMJFAQSA-N 0.000 description 1
- DBSLVQBXKVKDKJ-BJDJZHNGSA-N Leu-Ile-Ala Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O DBSLVQBXKVKDKJ-BJDJZHNGSA-N 0.000 description 1
- LXKNSJLSGPNHSK-KKUMJFAQSA-N Leu-Leu-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)O)N LXKNSJLSGPNHSK-KKUMJFAQSA-N 0.000 description 1
- MVHXGBZUJLWZOH-BJDJZHNGSA-N Leu-Ser-Ile Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O MVHXGBZUJLWZOH-BJDJZHNGSA-N 0.000 description 1
- SBANPBVRHYIMRR-GARJFASQSA-N Leu-Ser-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CO)C(=O)N1CCC[C@@H]1C(=O)O)N SBANPBVRHYIMRR-GARJFASQSA-N 0.000 description 1
- 102000009151 Luteinizing Hormone Human genes 0.000 description 1
- 108010073521 Luteinizing Hormone Proteins 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- XFIHDSBIPWEYJJ-YUMQZZPRSA-N Lys-Ala-Gly Chemical compound OC(=O)CNC(=O)[C@H](C)NC(=O)[C@@H](N)CCCCN XFIHDSBIPWEYJJ-YUMQZZPRSA-N 0.000 description 1
- FACUGMGEFUEBTI-SRVKXCTJSA-N Lys-Asn-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CCCCN FACUGMGEFUEBTI-SRVKXCTJSA-N 0.000 description 1
- SFQPJNQDUUYCLA-BJDJZHNGSA-N Lys-Cys-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CS)NC(=O)[C@H](CCCCN)N SFQPJNQDUUYCLA-BJDJZHNGSA-N 0.000 description 1
- RZHLIPMZXOEJTL-AVGNSLFASA-N Lys-Gln-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CCCCN)N RZHLIPMZXOEJTL-AVGNSLFASA-N 0.000 description 1
- WGLAORUKDGRINI-WDCWCFNPSA-N Lys-Glu-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O WGLAORUKDGRINI-WDCWCFNPSA-N 0.000 description 1
- ITWQLSZTLBKWJM-YUMQZZPRSA-N Lys-Gly-Ala Chemical compound OC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](N)CCCCN ITWQLSZTLBKWJM-YUMQZZPRSA-N 0.000 description 1
- BDFHWFUAQLIMJO-KXNHARMFSA-N Lys-Thr-Pro Chemical compound C[C@H]([C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCCCN)N)O BDFHWFUAQLIMJO-KXNHARMFSA-N 0.000 description 1
- ZNAPAUSAUBHENO-IHPCNDPISA-N Lys-Trp-His Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CC3=CN=CN3)C(=O)O)NC(=O)[C@H](CCCCN)N ZNAPAUSAUBHENO-IHPCNDPISA-N 0.000 description 1
- YORIKIDJCPKBON-YUMQZZPRSA-N Met-Glu-Gly Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O YORIKIDJCPKBON-YUMQZZPRSA-N 0.000 description 1
- SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 description 1
- 108010077850 Nuclear Localization Signals Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- SSSFPISOZOLQNP-GUBZILKMSA-N Pro-Arg-Asp Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(O)=O SSSFPISOZOLQNP-GUBZILKMSA-N 0.000 description 1
- DMKWYMWNEKIPFC-IUCAKERBSA-N Pro-Gly-Arg Chemical compound [H]N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O DMKWYMWNEKIPFC-IUCAKERBSA-N 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 238000010818 SYBR green PCR Master Mix Methods 0.000 description 1
- LRZLZIUXQBIWTB-KATARQTJSA-N Ser-Lys-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O LRZLZIUXQBIWTB-KATARQTJSA-N 0.000 description 1
- XKFJENWJGHMDLI-QWRGUYRKSA-N Ser-Phe-Gly Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)NCC(O)=O XKFJENWJGHMDLI-QWRGUYRKSA-N 0.000 description 1
- DINQYZRMXGWWTG-GUBZILKMSA-N Ser-Pro-Pro Chemical compound OC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 DINQYZRMXGWWTG-GUBZILKMSA-N 0.000 description 1
- UXRMWRBWCAGDQB-UHFFFAOYSA-N Theaflavin Natural products C1=CC(C2C(CC3=C(O)C=C(O)C=C3O2)O)=C(O)C(=O)C2=C1C(C1OC3=CC(O)=CC(O)=C3CC1O)=CC(O)=C2O UXRMWRBWCAGDQB-UHFFFAOYSA-N 0.000 description 1
- PXQUBKWZENPDGE-CIQUZCHMSA-N Thr-Ala-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](C)NC(=O)[C@H]([C@@H](C)O)N PXQUBKWZENPDGE-CIQUZCHMSA-N 0.000 description 1
- AYCQVUUPIJHJTA-IXOXFDKPSA-N Thr-His-Leu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(C)C)C(O)=O AYCQVUUPIJHJTA-IXOXFDKPSA-N 0.000 description 1
- KRDSCBLRHORMRK-JXUBOQSCSA-N Thr-Lys-Ala Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O KRDSCBLRHORMRK-JXUBOQSCSA-N 0.000 description 1
- ABWNZPOIUJMNKT-IXOXFDKPSA-N Thr-Phe-Ser Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(O)=O ABWNZPOIUJMNKT-IXOXFDKPSA-N 0.000 description 1
- DXDMNBJJEXYMLA-UBHSHLNASA-N Trp-Asn-Asp Chemical compound C1=CC=C2C(C[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O)=CNC2=C1 DXDMNBJJEXYMLA-UBHSHLNASA-N 0.000 description 1
- PVRRBEROBJQPJX-SZMVWBNQSA-N Trp-His-Gln Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CC3=CN=CN3)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N PVRRBEROBJQPJX-SZMVWBNQSA-N 0.000 description 1
- RRVUOLRWIZXBRQ-IHPCNDPISA-N Trp-Leu-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC1=CNC2=CC=CC=C21)N RRVUOLRWIZXBRQ-IHPCNDPISA-N 0.000 description 1
- DTPWXZXGFAHEKL-NWLDYVSISA-N Trp-Thr-Glu Chemical compound [H]N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(O)=O DTPWXZXGFAHEKL-NWLDYVSISA-N 0.000 description 1
- IYHRKILQAQWODS-VJBMBRPKSA-N Trp-Trp-Glu Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CC3=CNC4=CC=CC=C43)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N IYHRKILQAQWODS-VJBMBRPKSA-N 0.000 description 1
- YMZYSCDRTXEOKD-IHPCNDPISA-N Tyr-Trp-Asn Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CC3=CC=C(C=C3)O)N YMZYSCDRTXEOKD-IHPCNDPISA-N 0.000 description 1
- VLOYGOZDPGYWFO-LAEOZQHASA-N Val-Asp-Glu Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O VLOYGOZDPGYWFO-LAEOZQHASA-N 0.000 description 1
- XEYUMGGWQCIWAR-XVKPBYJWSA-N Val-Gln-Gly Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)NCC(=O)O)N XEYUMGGWQCIWAR-XVKPBYJWSA-N 0.000 description 1
- XXWBHOWRARMUOC-NHCYSSNCSA-N Val-Lys-Asn Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(=O)N)C(=O)O)N XXWBHOWRARMUOC-NHCYSSNCSA-N 0.000 description 1
- VPGCVZRRBYOGCD-AVGNSLFASA-N Val-Lys-Val Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O VPGCVZRRBYOGCD-AVGNSLFASA-N 0.000 description 1
- NHXZRXLFOBFMDM-AVGNSLFASA-N Val-Pro-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)C(C)C NHXZRXLFOBFMDM-AVGNSLFASA-N 0.000 description 1
- QIVPZSWBBHRNBA-JYJNAYRXSA-N Val-Pro-Phe Chemical compound CC(C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(O)=O QIVPZSWBBHRNBA-JYJNAYRXSA-N 0.000 description 1
- JKQXZKUSFCKOGQ-LQFQNGICSA-N Z-zeaxanthin Natural products C([C@H](O)CC=1C)C(C)(C)C=1C=CC(C)=CC=CC(C)=CC=CC=C(C)C=CC=C(C)C=CC1=C(C)C[C@@H](O)CC1(C)C JKQXZKUSFCKOGQ-LQFQNGICSA-N 0.000 description 1
- 101150034063 ZDS gene Proteins 0.000 description 1
- 101150067436 ZDS1 gene Proteins 0.000 description 1
- QOPRSMDTRDMBNK-RNUUUQFGSA-N Zeaxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCC(O)C1(C)C)C=CC=C(/C)C=CC2=C(C)CC(O)CC2(C)C QOPRSMDTRDMBNK-RNUUUQFGSA-N 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 108010047495 alanylglycine Proteins 0.000 description 1
- JKQXZKUSFCKOGQ-LOFNIBRQSA-N all-trans-Zeaxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2=C(C)CC(O)CC2(C)C JKQXZKUSFCKOGQ-LOFNIBRQSA-N 0.000 description 1
- KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000074 antisense oligonucleotide Substances 0.000 description 1
- 238000012230 antisense oligonucleotides Methods 0.000 description 1
- 108010059459 arginyl-threonyl-phenylalanine Proteins 0.000 description 1
- 108010062796 arginyllysine Proteins 0.000 description 1
- 108010040443 aspartyl-aspartic acid Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 235000020279 black tea Nutrition 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000007621 cluster analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 229920002770 condensed tannin Polymers 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000006356 dehydrogenation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 150000004141 diterpene derivatives Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- GVVPGTZRZFNKDS-JXMROGBWSA-N geranyl diphosphate Chemical compound CC(C)=CCC\C(C)=C\CO[P@](O)(=O)OP(O)(O)=O GVVPGTZRZFNKDS-JXMROGBWSA-N 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 108010055341 glutamyl-glutamic acid Proteins 0.000 description 1
- 108010015792 glycyllysine Proteins 0.000 description 1
- 230000033444 hydroxylation Effects 0.000 description 1
- 238000005805 hydroxylation reaction Methods 0.000 description 1
- 238000010813 internal standard method Methods 0.000 description 1
- 101150044508 key gene Proteins 0.000 description 1
- 108010077158 leucinyl-arginyl-tryptophan Proteins 0.000 description 1
- 108010057821 leucylproline Proteins 0.000 description 1
- 150000002634 lipophilic molecules Chemical class 0.000 description 1
- 229940040129 luteinizing hormone Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000030648 nucleus localization Effects 0.000 description 1
- 235000020333 oolong tea Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000010525 oxidative degradation reaction Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000003711 photoprotective effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000009894 physiological stress Effects 0.000 description 1
- 235000011765 phytoene Nutrition 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- CASUWPDYGGAUQV-UHFFFAOYSA-M potassium;methanol;hydroxide Chemical class [OH-].[K+].OC CASUWPDYGGAUQV-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108700042769 prolyl-leucyl-glycine Proteins 0.000 description 1
- 108010004914 prolylarginine Proteins 0.000 description 1
- 108010090894 prolylleucine Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000014620 theaflavin Nutrition 0.000 description 1
- IPMYMEWFZKHGAX-ZKSIBHASSA-N theaflavin Chemical compound C1=C2C([C@H]3OC4=CC(O)=CC(O)=C4C[C@H]3O)=CC(O)=C(O)C2=C(O)C(=O)C=C1[C@@H]1[C@H](O)CC2=C(O)C=C(O)C=C2O1 IPMYMEWFZKHGAX-ZKSIBHASSA-N 0.000 description 1
- 229940026509 theaflavin Drugs 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- 125000002640 tocopherol group Chemical class 0.000 description 1
- 235000019149 tocopherols Nutrition 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 108010073969 valyllysine Proteins 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 235000010930 zeaxanthin Nutrition 0.000 description 1
- 239000001775 zeaxanthin Substances 0.000 description 1
- 229940043269 zeaxanthin Drugs 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本发明属于基因工程技术领域,公开了CsMYB110基因在调控类胡萝卜素合成中的应用。其中CsMYB110基因的核苷酸序列如序列表中SEQ ID NO.1所示;CsMYB110基因编码的蛋白的氨基酸序列如序列表中SEQ ID NO.2所示。CsMYB110的表达模式与茶树叶片中类胡萝卜素合成高度相关,该基因能够显著促进植物的类胡萝卜素合成积累。该基因的克隆将不仅有利于解析茶树叶片类胡萝卜素的调控机制,而且有助于培育类胡萝卜素含量较高的茶树品种,提高茶叶品质,具有很大的应用价值。
Description
技术领域
本发明涉及基因工程技术领域,具体为CsMYB110基因在调控类胡萝卜素合成中的应用。
背景技术
茶树(Camellia sinensis(L.)O.Kuntze)是我国重要的经济作物,其叶片中的色素主要分为脂溶性色素和水溶性色素两大类,其中黄酮类、花青素类以及茶黄素等属于水溶性色素,而叶绿素,类胡萝卜素,原花色素等属于脂溶性色素。这些色素都与茶叶品质质量密切相关,包括茶叶的干茶、茶汤及叶底的色泽以及茶叶香气的形成。
类胡萝卜素广泛存在于高等植物的叶、花和果实中,是一种天然的黄色、橙红色或红色的亲脂分子,属于萜类色素。茶叶中类胡萝卜素在每100g干重36~73mg之间,主要由β-胡萝卜素、叶黄素和玉米黄质组成,在植物的生理、发育、生态和进化中起到重要作用。在高等植物中,类胡萝卜素在质体中合成,首先从甲羟戊酸途径(MEP)途径开始,由丙酮酸和3-磷酸甘油醛合成异戊二烯焦磷酸(IPP)及其异构体二甲烯丙基焦磷酸(DMAPP),然后三个IPP分子与一个DMAPP分子通过牻牛儿基焦磷酸合成酶(GGPPS)缩合成牻牛儿基牻牛儿基焦磷酸(GGPP),这个途径是二萜类化合物、类胡萝卜素、生育酚和叶绿素侧链合成过程中共有的。八氢番茄红素合成酶(PSY)催化两分子的GGPP缩合生成C40八氢番茄红素,这是生成的第一个类胡萝卜素化合物,然后再经过脱氢、环化、羟基化等转变为其他的类胡萝卜素,如β-胡萝卜素、叶黄素等。近年来,人们对类胡萝卜素的生物合成途径进行了广泛研究,并且已经在许多植物中鉴定了大部分催化生物合成步骤的酶基因,但是对于调控机制的研究大多集中在花与果实方面,对于叶片、种子以及根中研究较少。
通常来说,消费者对茶类饮品的青睐很大一部分是取决于其香气与味道,而大多数香气化合物是在茶类的加工过程中由类胡萝卜素、氨基酸、不饱和脂肪酸等转化形成的。类胡萝卜素在茶树叶片中含量较高,不仅是植物光合作用和光保护所必需的色素,参与光合作用以及多种生理逆境调节,而且是形成茶叶色泽和香气的重要化合物,其氧化降解产物如β-紫罗酮、二氢海葵内脂等对增进茶叶香气有重要作用。有研究表明,类胡萝卜素在生产过程中的降解导致了红茶与乌龙茶加工过程中萜类风味化合物的形成,由此鉴定类胡萝卜素是生产高品质茶类香气的重要前体物质。近年来茶树基因组数据的公布,虽然为我们从分子水平解析茶树叶片中类胡萝卜素合成机理提供了理论参考,但是在基因层次研究茶树类胡萝卜素合成的研究较少,不能够为基因工程育种提供优秀的基因资源。
发明内容
本发明的目的在于:提供CsMYB110基因在调控类胡萝卜素合成中的应用,实现调控茶树叶片中类胡萝卜素合成和茶叶品质形成的应用研究,为实现茶树选择性农艺性状育种提供了理论和实际参考基础。
为了实现上述目的,本发明提供如下技术方案:
一种CsMYB110基因,所述CsMYB110基因为茶树MYB类转录因子基因,所述基因的核苷酸序列,如序列表SEQ ID NO.1所示。
优选地,所述CsMYB110基因编码的蛋白序列,如序列表SEQ ID NO.2所示。
优选地,CsMYB110基因在调控类胡萝卜素合成中的应用,所述CsMYB110基因应用于调控茶树中类胡萝卜素合成和茶叶品质的形成。
本发明的有益效果在于:
本发明中,首次克隆并验证了调控茶树类胡萝卜素合成的关键转录因子CsMYB110,该转录因子在茶树中促进类胡萝卜素类化合物合成,影响茶叶品质形成,本发明还提供了含有CsMYB110基因的重组质粒、转基因工程菌和转基因植株。本发明丰富了茶树次生代谢的认知,以及茶叶品质形成特别是香气形成的遗传机制。本发明为实现茶树选择性农艺性状育种提供了理论和实际参考基础。
附图说明
图1:为茶树不同组织中类胡萝卜素化合物的含量差异和CsMYB110以及类胡萝卜素合成基因的表达差异图;
图2:为CsMYB110基因在烟草叶片中的亚细胞定位分析图;
图3:为CsMYB110基因促进烟草叶片中类胡萝卜素化合物的合成比较图;
图4:为CsMYB110基因调控茶树叶片类胡萝卜素化合物合成积累图。
具体实施方式
在本发明中所使用的术语,除非有另外说明,一般具有本领域普通技术人员通常理解的含义。
下面结合具体的制备实施例和应用实施例,并参照数据进一步详细地描述本发明。应理解,这些实施例只是为了举例说明本发明,而非以任何方式限制本发明的范围。
在以下的实施例中,未详细描述的各种过程和方法是本领域中公知的常规方法。所用到的引物,均在首次出现时标明,其后所用相同引物,均以首次标明的内容相同。
实施例1:
1、CsMYB110基因的克隆与序列结构分析
CsMYB110基因,为茶树MYB类转录因子基因,其克隆与序列结构分析,具体如下:
茶树国家级良种舒茶早种植于安徽省庐阳区合肥安徽农业大学农业产业园,取幼嫩叶片用于RNA的提取。总RNA的抽提采用Trizol试剂(Invitrogen,USA)按照说明操作,用分光度计检测其RNA含量和质量。
反转录生成第一链:取1μg RNA作模板,根据Promega公司M-MLV1反转录酶配套试剂使用说明,分别加入下游引物0.5μg,定容至15μl,70℃变性5min,立即在冰上放置5min;然后分别加入M-MLV 5×buffer 5μl,dNTP(25mM)5μl,rRNasin RibonucleaseInhibitor25U,M-MLV反转录酶200U,DEPC水补足25μl,42℃温育1h,95℃5min灭活反转录酶。经过优化后,取适量的反转录产物用于随后的PCR。以cDNA第一链作为RT-PCR模板,常规方法做PCR,扩增CsMYB110基因。其中上游引物:(5’-AGGTGCATGGACTGAGGAAG-3’),下游引物:(5’-CAAAGATCCCAAAGGTCCATTTT-3’)。25μl PCR反应体系为:10×Ex taq buffer 2.5μl,dNTP 2.0μl,Mg2+1.5μl,上、下游引物各1μl,Ex taq 0.2μl,模板1μl,ddH20 15.8μl。反应程序如下为95℃5min,95℃50sec,58℃50sec,72℃1min,72℃10min,35个循环。PCR产物CsMYB110基因经纯化回收后,连接到pMDTM19-T Simple Vector载体(Takara,Japan)上得到pMDTM19-T-CsMYB110质粒,转化大肠杆菌感受态细胞DH5α,送上海生工公司测序,得到的CsMYB110基因的核苷酸序列如序列表SEQ ID NO.1所示,具体如下:
ATGGAAGGTGTTCCTTTAGGAGTGAGAAAAGGTGCATGGACTGAGGAAGAAGACAACCTCCTTAAGAAGTGCATTGAAACTAATGGAGAAGGAAAGTGGCACCAAGTTCCTTTCAAAGCAGGATTGAACAGATGCAGGAAGAGTTGTAGATTGAGATGGTTGAACTATCTGAGGCCCAATATTAAGAGAGGAAGCTTTGGGGTAGATGAAGTTGATCTCATTGTTAGGCTTCATAAGCTTCTAGGAAACAGATGGTCGCTAATTGCGGGCAGACTTCCAGGAAGGACAGCAAACGATGTAAAAAACTACTGGAATACCCACTTGCAGAAGAAGCTGATACCTCAAATTGAAGTGGTGAAAGTTAAGACTCCGAGGATGATGGAGACCAAAGCTATACGACCTCGACCTCGAACCTTCTCAAAAAACCTAATTTGGTTAAAATCCAAAACAACCGCCATAGCTAATATTGAAACAAGAAACAATCTCTTCAAGCAACTATCACCACCACTATCGCCGCCAAGGGACGATGGAATATCGTGGTGGGAAAACATGTTTGTTGACTTGGAAATTAACAAAGAAATCACATTGTCAATTGATGGATCAAACGAGGAGAAATGGCATGAAAAGGAAACACAAGGTATTCTGGCAATTGGCGACAGTTCTGTTCAAGGAGAGAGTGATTGGAATGACATTTTTATTGATAAAATGGACCTTTGGGATCTTTGA。
CsMYB110基因编码的蛋白序列,如序列表SEQ ID NO.2所示具体如下:
MEGVPLGVRKGAWTEEEDNLLKKCIETNGEGKWHQVPFKAGLNRCRKSCRLRWLNYLRPNIKRGSFGVDEVDLIVRLHKLLGNRWSLIAGRLPGRTANDVKNYWNTHLQKKLIPQIEVVKVKTPRMMETKAIRPRPRTFSKNLIWLKSKTTAIANIETRNNLFKQLSPPLSPPRDDGISWWENMFVDLEINKEITLSIDGSNEEKWHEKETQGILAIGDSSVQGESDWNDIFIDKMDLWDL。
2、茶树不同组织类胡萝卜素含量和CsMYB110基因表达分析
(1)茶树不同组织类胡萝卜素含量分析
茶树国家级良种舒茶早种植于安徽省庐阳区合肥安徽农业大学农业产业园,通过8个组织器官用来分析类胡萝卜素含量分布和基因表达。这8个组织器官包括顶芽、嫩叶、成熟叶、老叶、嫩茎、花、果实、根。类胡萝卜素化合物测定采用安捷伦高效液相色谱仪(HPLC)进行分析。
取新鲜研磨的样品粉末约0.1g,加入1ml氯仿匀混,室温超声仪处理1h;再加入800μl水混匀,2500r/min离心10min,分层后取下部有色液体至新管中,真空干燥至液体挥发;加入500μl异丙醇和100μl饱和的KOH-甲醇溶液混匀避光放置于-20℃过夜;第二天加入500μl水和400μl氯仿混匀,2500r/min离心10min,分层后取下部有色液体至新管中,真空干燥至液体挥发;加入少量乙酸乙酯混匀,用0.22μm滤膜过滤,然后分装到进样瓶中准备进样分析。
HPLC参数设置如下:反相Spherisorb ODS2色谱柱(5μm,4.6×250mm)(Waters),柱温30℃,进样量为10μl,检测波长为440nm,采用二元流动相,流速为1.0mL/min,梯度洗脱(A80%甲醇+,B 100%乙酸乙酯:0.1-5min 95%A 5%B;5min-8min 75%A 25%B;8min-15min 70%A 30%B;15min-25min 60%A 40%B;25min-45min55%A 45%B;45min-60min45%A 55%B;60min-65min 30%A 70%B;65min-70min 100%B;70min-75min 95%A 5%B)。所检测物质均采用内标法进行定性和定量。
(2)CsMYB110和相关基因在茶树不同组织中表达分析
上文中茶树8个不同组织用于总RNA的提取,然后交由中国华大公司进行二代转录组测序,每个样品三个生物学重复。基因表达量计算由标准的转录组测序分析流程产生,基因表达量用FPKM(Fragments Per Kilobase of exon model per Million mappedfragments)值表示。
图1为茶树不同组织中类胡萝卜素化合物的含量差异和CsMYB110以及类胡萝卜素合成基因的表达差异图。如图1所示,通过HPLC分析可以看到茶树8个不同组织的叶黄素与β-胡萝卜素含量分布不同,在老叶(ML)中叶黄素含量最高,并且随叶片成熟度不断升高而升高,β-胡萝卜素也呈现类似趋势。在茶树嫩茎(ST)、嫩芽(AB)、花(FL)中类胡萝卜素含量也比较高,在其他组织中含量比较低。其中茶树花中只特异性地含有β-胡萝卜素。通过基因表达聚类分析表明,CsMYB110与一些胡萝卜素代谢途径结构基因具有相似的表达趋势,都高表达在茶树花中。
3、CsMYB110基因亚细胞定位和烟草叶片瞬时过量表达分析
(1)CsMYB110-pK7GW7载体构建
以pMDTM 19-T Simple:CsMYB110质粒为模板,利用引物(其中上游引物为
5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCAGGTGCATGGACTGAGGAAG-3’,下游引物为5’-GGGGACCACTTTGTACAAGAAAGCTGGGTCAAAGATCCCAAAGGTCCATTTT-3’)进行PCR扩增。PCR产物用1%的琼脂糖胶电泳条带进行回收。利用Gateway克隆技术,加入1μl PCR回收产物,与其质量相当的pDONR221中间载体,最后加入1μl BP Clonase Mix,室温过夜后转化DH5α,送上海生工公司测序。测序正确的阳性克隆的质粒,取1μl质粒并加入等量pK7GW7过表达载体,最后加入1μl LR Clonase Mix,室温过夜后转化DH5α,送上海生工公司测序验证。
(2)CsMYB110亚细胞定位分析
取适量的本式烟草种子加去离子水,置于4℃冰箱春化处理72h之后播种。播种完覆盖保鲜膜,置于合适条件(湿度60%,温度23℃,16小时光照/8小时黑暗)下等待发芽。种子出芽之后,尽量选取大小一致的苗进行移栽,正常培养。把CsMYB110-pK7GW7载体电激法转化到GV3101农杆菌当中,并通过常规PCR方法鉴定阳性克隆。挑取含有目的基因的阳性菌落,在5mL含有相应抗生素的LB液体培养基中,28℃,200r/min培养约24h;吸取培养过的菌液2ml,加入到50mL新鲜的含有相应抗生素的LB液体培养基,继续振荡培养至OD600约为1.0,离心收集菌体,用适量转化液(MS+0.3mg/L6-BA+150g/L蔗糖+15g/LEMS+0.06%silwetL-77,PH=5.7)重悬菌体,终浓度OD600约为0.8。将准备好的转化液装在注射器中,轻轻地注射到烟草叶片背部,然后黑暗放置24H。三天之后,取注射的叶片进行激光共聚焦显微镜观察。以不含有目的基因的空载体作为对照。
图2为CsMYB110基因在烟草叶片中的亚细胞定位分析图。如图2所示,CsMYB110在烟草叶片中具有明显的转录因子核定位信号,这说明CsMYB110可能在细胞核中调控相关基因的转录表达。
(3)烟草叶片瞬时表达代谢物分析
通过激光共聚焦显微镜对烟草叶片进行荧光分析,选择带有核定位荧光信号的烟草叶片作为CsMYB110瞬时过表达遗传材料,空载体作为对照。然后用HPLC分别分析烟草叶片中类胡萝卜素化合物含量的差异。
图3为CsMYB110基因促进烟草叶片中类胡萝卜素化合物的合成比较图。图3所示,图3(A)中,在烟草叶片中瞬时表达CsMYB110,能显著促进β胡萝卜素的合成。图3(B)中,在烟草叶片中瞬时表达CsMYB110,能显著促进黄体黄质的合成。在图3中,把CsMYB110瞬时过表达在烟草叶片中,能显著提高烟草叶片的类胡萝卜素化合物的合成积累,其中黄体黄质和β-胡萝卜素升高最显著。
结果表明,瞬时过表达CsMYB110能显著提高烟草叶片中类胡萝卜素化合物的含量。
4、CsMYB110基因在茶树体内功能验证
(1)体外寡核苷酸反义抑制实验
根据CsMYB110预测序列设计合成寡核苷酸反义的引物,设计在网站http://sfold.wadsworth.org/cgi-bin/soligo.pl上完成,引物序列如所示:
P1,(5’-GTTGTCTTCTTCCTCAGTCC-3’);
P2,(5’-GGTCGTATAGCTTTGGTCTC-3’);
P3,(5’-CATCGTTTGCTGTCCTTCCT-3’);
用80mM蔗糖溶液溶解,配制并获得体外寡核苷酸反义抑制缓冲液,空白为蔗糖溶液;用剪刀剪下大小基本一致,颜色鲜艳,色泽健康,无虫无病的一芽一叶,***装有缓冲液的96孔板中,要确保一芽一叶尾部没入缓冲液中。将96孔板放入光照培养箱中按光照16h/黑暗8h进行光照培养,培养箱温度为28℃。处理后3d引物处理样和空白样分别取样进行代谢和基因表达分析。
(2)体外寡核苷酸反义抑制样品的代谢物和相关基因表达分析
对处理样品和对照样品分别用于总RNA的提取以及cDNA第一链合成。反转录产物(cDNA第一条链)稀释80倍作为模板,使用SYBR Realtime Mix(TOYOBO,Osaka,Japan),配制20μl反应体系:8.4μl稀释80倍的逆转录产物,上下游引物各0.8μl(10pmol/μl),10μl2×SYBR Green PCR Master Mix,每个反应配3个重复。然后在bio-radCFX-96上以程序:①95℃3min;②95℃10s,60℃15s,72℃30s运行45个循环;③从65℃到95℃,以0.1℃/s绘制熔解曲线。
上游引物:(5’-ACCTCGACCTCGAACCTTCT-3’),下游引物:(5’-TATTCCATCGTCCCTTGGCG-3’),以茶树ACTIN基因为内参,上游引物:(5’-GCCATATTTGATTGGAATGG-3’),下游引物:(5’-GGTGCCACAACCTTGATCTT-3’)通过仪器自带分析软件,计算出CsMYB110的相对表达数值。同理,计算PSY(上游引物5’-GTGGCGAAGTTTGTGCAGAG-3’,下游引物:5’-GTGACGCATTAGGCCCATCA-3’)、PDS(上游引物5’-TTTGGCAGATGCAGGTCACA-3’,下游引物:5’-TCAAATCGGCTGAACTCCCC-3’)、ZDS(上游引物5’-GCCATGAGGTTGGTGCTGAT-3’,下游引物:5’-GATCAACAAGAGCCCGCACA-3’)、CRTISO(上游引物5’-TTAGGGTTTAATGGGGCGGG-3’,下游引物:5’-AAACCCAGAACTCCCACCAG-3’)、LYCe(上游引物5’-GGCGCTTATTCTGCAACTGG-3’,下游引物:5’-TCGGCGGAGGAAAGAGTAGA-3’)、BCH(上游引物5’-ACGACGGTCTCGTTCACAAA-3’,下游引物:5’-CAACTCATCAAGCCCTCCCA-3’)的相对表达量。结果显示CsMYB110体外寡核苷酸反义抑制能显著干扰目的基因表达水平。类胡萝卜素合成关键基因表达分析显示:PSY、PDS、ZDS、CRTISO、LYCe、BCH等基因表达都显著下调。代谢物含量分析表明叶黄素及β-类胡萝卜素化合物含量显著降低。
图4为CsMYB110基因调控茶树叶片类胡萝卜素化合物合成积累图。其中图4(A)为寡核苷酸反义抑制实验示意图,一芽一叶材料取自于舒茶早品种,Control treatment,空白对照;AsODN-CsMYB110,CsMYB110寡核苷酸反义链抑制处理。图4(B)为CsMYB110基因在处理样品中的基因表达量图。图4(C)为茶树叶片中类胡萝卜素化合物含量变化图。图4(D)为类胡萝卜素代谢途径关键基因表达量变化图。
如图4所示,可以看到通过体外寡核苷酸反义抑制实验可以使得CsMYB110的表达量和对照相比显著被抑制,通过体外寡核苷酸反义抑制CsMYB110基因表达3d后代谢物质测定结果可以看到,抑制CsMYB110的表达可以显著抑制茶树叶片类胡萝卜素化合物的合成积累,类胡萝卜素合成途径中的关键结构基因的表达量也显著降低。
综上所述,CsMYB110蛋白及其编码基因与茶树叶片类胡萝卜素化合物合成代谢调控相关,能显著提高茶叶品质形成,CsMYB110基因能够应用于调控茶树中类胡萝卜素合成和茶叶品质的形成。
CsMYB110的表达模式与茶树叶片中类胡萝卜素合成高度相关,将该基因瞬时过量表达在植物叶片中,能够显著促进植物的类胡萝卜素合成积累,通过反义寡核苷酸技术抑制CsMYB110的表达,使茶树叶片中类胡萝卜素相关化合物含量显著下降,类胡萝卜素合成相关基因表达也显著下降。该基因的克隆将不仅有利于解析茶树叶片类胡萝卜素的调控机制,而且有助于培育类胡萝卜素含量较高的茶树品种,提高茶叶品质,具有很大的应用价值。
本发明中,首次克隆并验证了调控茶树类胡萝卜素合成的关键转录因子CsMYB110,该转录因子在茶树中促进类胡萝卜素类化合物合成,影响茶叶品质形成,本发明还提供了含有CsMYB110基因的重组质粒、转基因工程菌和转基因植株。本发明丰富了茶树次生代谢的认知,以及茶叶品质形成特别是香气形成的遗传机制。本发明为实现茶树选择性农艺性状育种提供了理论和实际参考基础。
上述是对发明进行了示例性描述,显然本发明具体实现并不受上述方式的限制,只要采用了本发明的方法构思和技术方案进行的这种非实质改进,或未经改进将发明的构思和技术方案直接应用于其他场合的,均在本发明的保护范围之内。
序列表
<110> 安徽农业大学
<120> CsMYB110基因及在调控类胡萝卜素合成中的应用
<130> NO
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 726
<212> DNA
<213> 茶树(Camellia sinensis L. O. Kuntze)
<400> 1
atggaaggtg ttcctttagg agtgagaaaa ggtgcatgga ctgaggaaga agacaacctc 60
cttaagaagt gcattgaaac taatggagaa ggaaagtggc accaagttcc tttcaaagca 120
ggattgaaca gatgcaggaa gagttgtaga ttgagatggt tgaactatct gaggcccaat 180
attaagagag gaagctttgg ggtagatgaa gttgatctca ttgttaggct tcataagctt 240
ctaggaaaca gatggtcgct aattgcgggc agacttccag gaaggacagc aaacgatgta 300
aaaaactact ggaataccca cttgcagaag aagctgatac ctcaaattga agtggtgaaa 360
gttaagactc cgaggatgat ggagaccaaa gctatacgac ctcgacctcg aaccttctca 420
aaaaacctaa tttggttaaa atccaaaaca accgccatag ctaatattga aacaagaaac 480
aatctcttca agcaactatc accaccacta tcgccgccaa gggacgatgg aatatcgtgg 540
tgggaaaaca tgtttgttga cttggaaatt aacaaagaaa tcacattgtc aattgatgga 600
tcaaacgagg agaaatggca tgaaaaggaa acacaaggta ttctggcaat tggcgacagt 660
tctgttcaag gagagagtga ttggaatgac atttttattg ataaaatgga cctttgggat 720
ctttga 726
<210> 2
<211> 241
<212> PRT
<213> 茶树(Camellia sinensis L. O. Kuntze)
<400> 2
Met Glu Gly Val Pro Leu Gly Val Arg Lys Gly Ala Trp Thr Glu Glu
1 5 10 15
Glu Asp Asn Leu Leu Lys Lys Cys Ile Glu Thr Asn Gly Glu Gly Lys
20 25 30
Trp His Gln Val Pro Phe Lys Ala Gly Leu Asn Arg Cys Arg Lys Ser
35 40 45
Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro Asn Ile Lys Arg Gly
50 55 60
Ser Phe Gly Val Asp Glu Val Asp Leu Ile Val Arg Leu His Lys Leu
65 70 75 80
Leu Gly Asn Arg Trp Ser Leu Ile Ala Gly Arg Leu Pro Gly Arg Thr
85 90 95
Ala Asn Asp Val Lys Asn Tyr Trp Asn Thr His Leu Gln Lys Lys Leu
100 105 110
Ile Pro Gln Ile Glu Val Val Lys Val Lys Thr Pro Arg Met Met Glu
115 120 125
Thr Lys Ala Ile Arg Pro Arg Pro Arg Thr Phe Ser Lys Asn Leu Ile
130 135 140
Trp Leu Lys Ser Lys Thr Thr Ala Ile Ala Asn Ile Glu Thr Arg Asn
145 150 155 160
Asn Leu Phe Lys Gln Leu Ser Pro Pro Leu Ser Pro Pro Arg Asp Asp
165 170 175
Gly Ile Ser Trp Trp Glu Asn Met Phe Val Asp Leu Glu Ile Asn Lys
180 185 190
Glu Ile Thr Leu Ser Ile Asp Gly Ser Asn Glu Glu Lys Trp His Glu
195 200 205
Lys Glu Thr Gln Gly Ile Leu Ala Ile Gly Asp Ser Ser Val Gln Gly
210 215 220
Glu Ser Asp Trp Asn Asp Ile Phe Ile Asp Lys Met Asp Leu Trp Asp
225 230 235 240
Leu
Claims (1)
1.一种CsMYB110基因在调控类胡萝卜素合成中的应用,其特征在于,CsMYB110基因为茶树MYB类转录因子基因,所述CsMYB110基因的核苷酸序列,如序列表SEQ ID NO.1所示;所述CsMYB110基因应用于调控茶树中类胡萝卜素合成和茶叶品质的形成。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110425738.2A CN113122547B (zh) | 2021-04-20 | 2021-04-20 | CsMYB110基因在调控类胡萝卜素合成中的应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110425738.2A CN113122547B (zh) | 2021-04-20 | 2021-04-20 | CsMYB110基因在调控类胡萝卜素合成中的应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113122547A CN113122547A (zh) | 2021-07-16 |
CN113122547B true CN113122547B (zh) | 2022-03-18 |
Family
ID=76778232
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110425738.2A Active CN113122547B (zh) | 2021-04-20 | 2021-04-20 | CsMYB110基因在调控类胡萝卜素合成中的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113122547B (zh) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113234737A (zh) * | 2021-06-30 | 2021-08-10 | 安徽农业大学 | 茶树myb类转录因子基因在调控茶树咖啡碱生物合成方面的应用 |
CN114686493B (zh) * | 2022-04-25 | 2023-05-23 | 安徽农业大学 | CsJAZ12基因及在调控茶叶咖啡碱合成中的应用 |
CN116024227B (zh) * | 2022-08-29 | 2024-03-01 | 安徽农业大学 | 茶树CsMYB206基因以及在调控茶叶咖啡碱合成中的应用 |
CN115838407B (zh) * | 2022-11-09 | 2024-04-26 | 安徽农业大学 | CsHO1在调控茶叶中茶氨酸的积累中的应用 |
CN117126865B (zh) * | 2023-09-01 | 2024-01-23 | 宁夏农林科学院枸杞科学研究所 | 一种促进类胡萝卜素含量积累的LbaMYB44基因及应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1628127A (zh) * | 2002-03-27 | 2005-06-15 | 科学与工业研究委员会 | 来源于干旱压力耐受性茶树的基因和引入水分压力耐受性的方法 |
CN109678943A (zh) * | 2019-01-16 | 2019-04-26 | 淮阴工学院 | 蛋白AeZDS及其编码基因在提高植物类胡萝卜素积累和抗逆性中的应用 |
CN111187789A (zh) * | 2020-03-13 | 2020-05-22 | 南京农业大学 | 一种水稻myb转录因子及其重组表达载体的应用 |
CN112391398A (zh) * | 2020-11-30 | 2021-02-23 | 青岛市农业科学研究院 | 一种苹果黄酮糖基转移酶基因MdGT1及其应用 |
CN113234737A (zh) * | 2021-06-30 | 2021-08-10 | 安徽农业大学 | 茶树myb类转录因子基因在调控茶树咖啡碱生物合成方面的应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120137382A1 (en) * | 1998-09-22 | 2012-05-31 | Mendel Biotechnology, Inc. | Stress tolerance in plants |
NZ542110A (en) * | 2005-08-30 | 2008-07-31 | Horticulture & Food Res Inst | Compositions and methods for modulating pigment production in plants |
BR112014018294B1 (pt) * | 2012-01-26 | 2022-01-11 | Norfolk Plant Sciences, Ltd | Método para produzir uma planta, cassete de expressão, e, célula bacteriana |
CN104561025A (zh) * | 2013-10-29 | 2015-04-29 | 华中农业大学 | 番茄SlML1基因及应用 |
WO2019226938A1 (en) * | 2018-05-23 | 2019-11-28 | The United States Of America, As Represented By The Secretary Of Agriculture | Fruit-specific promoters |
CN110724696B (zh) * | 2019-11-19 | 2021-01-12 | 安徽农业大学 | 一种脂氢过氧化物裂解酶及其基因和用途 |
CN111100849B (zh) * | 2020-01-16 | 2021-01-12 | 安徽农业大学 | 茶树类胡萝卜素裂解双加氧酶CsCCD4及其在催化合成β-紫罗酮上的应用 |
-
2021
- 2021-04-20 CN CN202110425738.2A patent/CN113122547B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1628127A (zh) * | 2002-03-27 | 2005-06-15 | 科学与工业研究委员会 | 来源于干旱压力耐受性茶树的基因和引入水分压力耐受性的方法 |
CN109678943A (zh) * | 2019-01-16 | 2019-04-26 | 淮阴工学院 | 蛋白AeZDS及其编码基因在提高植物类胡萝卜素积累和抗逆性中的应用 |
CN111187789A (zh) * | 2020-03-13 | 2020-05-22 | 南京农业大学 | 一种水稻myb转录因子及其重组表达载体的应用 |
CN112391398A (zh) * | 2020-11-30 | 2021-02-23 | 青岛市农业科学研究院 | 一种苹果黄酮糖基转移酶基因MdGT1及其应用 |
CN113234737A (zh) * | 2021-06-30 | 2021-08-10 | 安徽农业大学 | 茶树myb类转录因子基因在调控茶树咖啡碱生物合成方面的应用 |
Also Published As
Publication number | Publication date |
---|---|
CN113122547A (zh) | 2021-07-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN113122547B (zh) | CsMYB110基因在调控类胡萝卜素合成中的应用 | |
Xie et al. | Supplemental blue and red light promote lycopene synthesis in tomato fruits | |
Stauder et al. | Strigolactone levels in dicot roots are determined by an ancestral symbiosis-regulated clade of the PHYTOENE SYNTHASE gene family | |
Harris et al. | Betalain production is possible in anthocyanin-producing plant species given the presence of DOPA-dioxygenase and L-DOPA | |
Ananga et al. | Production of anthocyanins in grape cell cultures: a potential source of raw material for pharmaceutical, food, and cosmetic industries | |
Park et al. | Enhanced accumulation of carotenoids in sweetpotato plants overexpressing IbOr-Ins gene in purple-fleshed sweetpotato cultivar | |
CN113151305B (zh) | 茶树wrky29基因在提高植物抗寒性上的应用 | |
Wang et al. | Exogenous abscisic acid regulates distribution of 13C and 15N and anthocyanin synthesis in ‘Red Fuji’apple fruit under high nitrogen supply | |
CN110607310B (zh) | 一种调控茶叶茶毫形成的基因及应用 | |
Baranski et al. | Carotenoid biosynthesis and regulation in plants | |
Meng et al. | Characterization of transcriptional expression and regulation of carotenoid cleavage dioxygenase 4b in grapes | |
CN113957086B (zh) | SlBIN2基因在调控番茄果实成熟和类胡萝卜素合成中的应用 | |
CN111100849B (zh) | 茶树类胡萝卜素裂解双加氧酶CsCCD4及其在催化合成β-紫罗酮上的应用 | |
CN113061614B (zh) | 番茄SlWRKY35基因在提高番茄类胡萝卜素类化合物或/和叶绿素含量中的应用 | |
Zhou et al. | Insights into carotenoid accumulation using VIGS to block different steps of carotenoid biosynthesis in petals of California poppy | |
JP2001000192A (ja) | ヒマワリファルネシルピロリン酸シンターゼ遺伝子を有する植物ベクターとその製造方法 | |
Ding et al. | AgZDS, a gene encoding ζ-carotene desaturase, increases lutein and β-carotene contents in transgenic Arabidopsis and celery | |
Wang et al. | The terpene synthase (TPS) gene family in kiwifruit shows high functional redundancy and a subset of TPS likely fulfil overlapping functions in fruit flavour, floral bouquet and defence | |
Jiang et al. | Characterization of BoaCRTISO reveals its role in carotenoid biosynthesis in Chinese kale | |
Jun-xing et al. | PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit | |
Cseke et al. | Regulation of metabolite synthesis in plants | |
Imtiaz et al. | Apocarotenoids biosynthesis, signaling regulation, crosstalk with phytohormone, and its role in stress tolerance | |
CN103820475B (zh) | 枸杞牦牛儿基牦牛儿焦磷酸合成酶基因及其编码的蛋白质和应用 | |
CN115948456A (zh) | 一种可提升广藿香醇合成量的融合基因及方法 | |
CN114032244B (zh) | 烟草NtPIF1基因及其编码蛋白和应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |