CN113116795A

CN113116795A - A cosmetic composition for stimulating collagen production and resisting wrinkle

Info

Publication number: CN113116795A
Application number: CN202110294315.1A
Authority: CN
Inventors: 曾启林
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-03-19
Filing date: 2021-03-19
Publication date: 2021-07-16

Abstract

The invention discloses a cosmetic composition for stimulating collagen to generate and resisting wrinkles, which comprises the following raw materials in parts by weight: 0.6-0.8 part of pinoresinol diglucoside, 1-3 parts of dipeptide diaminobutyrylbenzyl amide diacetate, 2-4 parts of sodium hyaluronate, 100 parts of deionized water, 3-5 parts of glycerol, 10-12 parts of aloe gel, 50-70 parts of plant extract, 2-4 parts of octyl glycol, 1-3 parts of 1, 2-hexanediol, 1.6-2 parts of palmitoyl tripeptide and 3-6 parts of a moisturizing antioxidant; the cosmetic composition for stimulating collagen production and resisting wrinkles is prepared by the following steps: the first step, preparing a first component; step two, preparing a second component; and thirdly, respectively heating the first component and the second component, then mixing, cooling, adding palmitoyl tripeptide, a moisturizing antioxidant, and uniformly stirring to obtain the cosmetic composition for stimulating the generation of collagen and resisting wrinkles.

Description

A cosmetic composition for stimulating collagen production and resisting wrinkle

Technical Field

The invention belongs to the technical field of cosmetic compositions, and particularly relates to a cosmetic composition for stimulating collagen to generate and resisting wrinkles.

Background

There is an increasing need in the cosmetics industry to develop products that can be topically applied to the skin to improve the condition and appearance of the skin. Consumer interest is in reducing or delaying the dermatological signs of chronologically or hormonally aged or photoaged skin, such as fine lines, wrinkles and sagging skin and other conditions resulting from the progressive degradation of the dermal-epidermal junction and cell-cell adhesion in the epidermis. During the aging process, the appearance of skin, i.e., the color and appearance of skin, deteriorates slowly from aging and/or exposure to sunlight. A number of cosmetic and medical treatments have been developed in an attempt to treat aging or aged skin. Such cosmetics or treatment methods typically contain an organic acid as its active ingredient or ingredient. These active ingredients often cause consumer discomfort such as burning, itching and redness.

Thus, there is still a general need in the cosmetics industry for products that retard or counteract the effects of aging of the skin. In particular, there remains a need for topically applied cosmetic compositions using natural plant materials as active ingredients that have anti-aging and skin texture benefits.

Disclosure of Invention

The present invention provides a cosmetic composition for stimulating collagen production to resist wrinkles.

The technical problems to be solved by the invention are as follows:

a large number of cosmetic and medical treatments are currently being developed in an attempt to treat aging or already aging skin. Such cosmetics or treatment methods typically contain an organic acid as its active ingredient or ingredient. These active ingredients often cause consumer discomfort such as burning, itching and redness.

The purpose of the invention can be realized by the following technical scheme:

a cosmetic composition for stimulating collagen generation and resisting wrinkles comprises the following raw materials in parts by weight:

0.6-0.8 part of pinoresinol diglucoside, 1-3 parts of dipeptide diaminobutyrylbenzyl amide diacetate, 2-4 parts of sodium hyaluronate, 100 parts of deionized water, 3-5 parts of glycerol, 10-12 parts of aloe gel, 50-70 parts of plant extract, 2-4 parts of octyl glycol, 1-3 parts of 1, 2-hexanediol, 1.6-2 parts of palmitoyl tripeptide and 3-6 parts of a moisturizing antioxidant;

the cosmetic composition for stimulating collagen production and resisting wrinkles is prepared by the following steps:

firstly, adding pinoresinol diglucoside, dipeptide diaminobutyryl benzylamide diacetate and sodium hyaluronate into deionized water, uniformly mixing, heating to 80-85 ℃, preserving heat, stirring and uniformly dispersing; regulating the pH value of the solution to 5.5 by using triethanolamine to obtain a first mixture;

secondly, mixing glycerol, aloe vera gel, plant extract, caprylyl glycol and 1, 2-hexanediol, and uniformly stirring at 40 ℃ to obtain a second mixture;

and step three, respectively heating the first mixture and the second mixture to 80 ℃, mixing and stirring at the rotating speed of 100r/min, cooling to 45 ℃, adding palmitoyl tripeptide and a moisturizing antioxidant, uniformly stirring, and storing at 25 ℃ to obtain the cosmetic composition for stimulating the generation of collagen and resisting wrinkles.

Further, the moisturizing antioxidant is prepared by the following steps:

step S11, mixing a sodium hydroxide solution and chitosan, standing for 10-12h at the temperature of-18 ℃ after mixing, then unfreezing and transferring to a three-neck flask, adding isopropanol into the three-neck flask, stirring for 30-40min at the temperature of 25 ℃ and the rotation speed of 400r/min, adding solid chloroacetic acid in an equivalent manner for three times, wherein the addition time is 5min, stirring and reacting for 2h at the temperature of 70 ℃ and the stirring speed of 400r/min, after the reaction is finished, adding equal-volume distilled water into the solution for diluting, adjusting the pH value to 7.0 by glacial acetic acid, carrying out vacuum filtration, washing a filter cake by using an ethanol aqueous solution with a volume fraction of 70% and absolute ethanol in sequence, and after the washing is finished, putting into a 60 ℃ drying oven for drying to obtain a solid a;

step S12, adding the solid a and N, N-dimethylformamide into a round-bottom flask, adding pyridine, setting the temperature to be 0-5 ℃, continuously stirring for 30min at the rotation speed of 300r/min, dropwise adding thionyl chloride mixed solution by using a constant-pressure dropping funnel, heating to 75 ℃ after dropwise adding, continuously stirring for reacting for 3h, mixing the obtained reaction solution with anhydrous ether with the same volume after the reaction is finished, separating out a precipitate, carrying out vacuum filtration, washing the obtained filter cake with acetone until the filtrate does not contain chloride ions, and after the washing is finished, carrying out vacuum drying at 50 ℃ to constant weight to obtain a solid b;

step S13, mixing solid b and phosphate buffer solution according to 1 g: mixing 50mL to obtain a mixed solution A, and mixing collagen peptide phosphate and a phosphate buffer solution according to the weight ratio of 1 g: 50mL of the mixture was mixed to obtain a mixed solution B, and the mixture was mixed with a microbial transglutaminase and a phosphate buffer solution in an amount of 0.1 g: mixing 20mL of the mixture to obtain a mixed solution C, mixing the mixed solution A, the mixed solution B and the mixed solution C, stirring for 10min at the temperature of 100 ℃, then cooling to room temperature, carrying out vacuum filtration, and carrying out vacuum drying on the obtained filter cake at the temperature of 25 ℃ to constant weight to obtain a first component;

further, in step S11, the sodium hydroxide solution is sodium hydroxide and deionized water, and the ratio of 1 g: 1mL of the sodium hydroxide solution and the chitosan are mixed, and the dosage ratio of the sodium hydroxide solution to the chitosan is 10 g: 10 mL; the dosage ratio of the chitosan, the isopropanol and the chloroacetic acid is 1 g: 10-15 mL: 1.2 g.

Further, in the step S12, the thionyl chloride mixed solution is thionyl chloride and N, N-dimethylformamide according to a volume ratio of 1: 1, mixing; the dosage ratio of the solid a, the N, N-dimethylformamide, the pyridine and the thionyl chloride mixed solution is 1 g: 5mL of: 5mL of: 20-30 mL.

Further, the phosphate buffer solution in step S13 is a 0.2mol/L Na2HPO4/NaH2PO4 buffer solution, and the pH value of the buffer solution is 6; the volume ratio of the mixed liquor A to the mixed liquor B to the mixed liquor C is 5: 5: 2.

step S21, adding dimethylacetamide into a three-neck flask, adding L-ascorbic acid and dichloromethane under the conditions that the temperature is 0 ℃ and the rotating speed is 200r/min, stirring for 6-10min, adding solid b for three times in different quantities, keeping the temperature and the rotating speed constant, continuing stirring for 50-60min, washing the obtained reaction solution with ethyl acetate at 0 ℃, washing with ice water until the filtrate does not contain chloride ions, after washing, drying in vacuum at 25 ℃ to constant weight to obtain a second component, mixing the first component and the second component according to a mass ratio of 1: 1 mixing to obtain the moisture-preserving antioxidant.

Further, the amount ratio of dimethylacetamide, L-ascorbic acid, dichloromethane and solid b in step S21 was 6 mL: 1 g: 2-3 mL: 1-1.2 g.

Further, the plant extract is prepared by the following steps:

step A11, weighing the following raw materials in parts by weight: 50-60 parts of strawberry leaves, 10-20 parts of angelica, 10-26 parts of pseudo-ginseng, 10-16 parts of motherwort, 10-20 parts of radix ophiopogonis and 10-15 parts of liquorice;

step A12, mixing the weighed raw materials, adding the mixture into a water-alcohol mixed solution, heating the mixture to 75-90 ℃, and extracting for 30-120 min;

step A13, stopping heating, cooling to 20-50 deg.C, filtering, concentrating under reduced pressure to remove solvent, and making into plant extract.

Further, in the step a12, the water-alcohol mixed solution is deionized water and propylene glycol according to a volume ratio of 2: 0.6-1 by weight; the dosage ratio of the weighed total amount of the raw materials to the water-alcohol mixed solution is 0.6-1 g: 20-30 mL.

The invention has the beneficial effects that:

the pinoresinol diglucoside in the first component can improve the proliferation activity of fibroblasts, promote the expression of related collagen, inhibit the expression of matrix metalloproteinases and reduce the degradation of collagen, and has a structure similar to that of estrogen and an estrogen-like effect. The phytoestrogen has the functions of promoting epidermal proliferation and collagen synthesis in vitro, protecting collagen from enzyme degradation, and promoting skin cells to generate collagen, thereby realizing skin repair and reducing wrinkle. Dipeptide diaminobutyrylbenzylamide diacetate is a synthetic peptide simulating snake venom serum, and can prevent acetylcholine nerve conduction, achieve the effect of relaxing muscles, and further improve dynamic wrinkles. The plant extract contains strawberry leaf extract, the strawberry leaf extract contains effective components for promoting fibroblast secretion and maturation, and the effective components cooperate with traditional Chinese medicine raw materials to protect skin.

Using chitosan with good biocompatibility as a matrix, firstly alkalifying the chitosan, then adding chloroacetic acid for many times for carboxymethylation to obtain a solid a, and performing acyl chlorination on the solid a to obtain a solid b; the solid b and the collagen peptide form isopeptide between primary amine on the solid b and amino on the collagen peptide under the catalytic action of glutaminase, the collagen peptide is grafted to chitosan, and the collagen peptide has stronger free radicals and excellent inoxidizability, and simultaneously can stimulate cell proliferation, stimulate collagen synthesis of skin cells and have moisturizing effect.

Solid b and the enol-type hydroxyl on the L-ascorbic acid are subjected to esterification reaction to prepare a second component, L-ascorbic acid is called vitamin C for short, the L-ascorbic acid is not only applied to the fields of food, agriculture and the like, but also applied to medical cosmetology, but 2 adjacent enol-type hydroxyl on C2 and C3 in the molecular structure of the L-ascorbic acid are extremely easy to decompose, and the properties of the L-ascorbic acid can be changed and limited by external influences.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The moisturizing antioxidant is prepared by the following steps:

step S11, mixing a sodium hydroxide solution and chitosan, standing for 11h at the temperature of-18 ℃ after mixing, then unfreezing and transferring to a three-neck flask, adding isopropanol into the three-neck flask, stirring for 35min at the temperature of 25 ℃ and the rotating speed of 400r/min, adding solid chloroacetic acid in three equal amounts, each time at an interval of 5min, stirring for reaction for 2h at the temperature of 70 ℃ and the stirring speed of 400r/min, after the reaction is finished, adding equal-volume distilled water into the solution for dilution, adjusting the pH value to 7.0 with glacial acetic acid, carrying out vacuum filtration, washing a filter cake with an ethanol aqueous solution with a volume fraction of 70% and absolute ethanol in sequence, and after the washing is finished, putting into a 60 ℃ oven for drying to obtain a solid a;

step S12, adding the solid a and N, N-dimethylformamide into a round-bottom flask, adding pyridine, setting the temperature at 2 ℃ and the rotating speed at 300r/min, continuing stirring for 30min, dropwise adding thionyl chloride mixed solution by using a constant-pressure dropping funnel, heating to 75 ℃ after dropwise adding, continuing stirring for reaction for 3h, after the reaction is finished, mixing the obtained reaction solution with equal volume of anhydrous ether, separating out a precipitate, performing vacuum filtration, washing the obtained filter cake with acetone until the filtrate does not contain chloride ions, and after the washing is finished, performing vacuum drying at 50 ℃ to constant weight to obtain a solid b;

step S13, mixing solid b and phosphate buffer solution according to 1 g: mixing 50mL to obtain a mixed solution A, and mixing collagen peptide phosphate and a phosphate buffer solution according to the weight ratio of 1 g: 50mL of the mixture was mixed to obtain a mixed solution B, and the mixture was mixed with a microbial transglutaminase and a phosphate buffer solution in an amount of 0.1 g: mixing 20mL of the mixture to obtain a mixed solution C, mixing the mixed solution A, the mixed solution B and the mixed solution C, stirring for 10min at the temperature of 100 ℃, then cooling to room temperature, carrying out vacuum filtration, and carrying out vacuum drying on the obtained filter cake at the temperature of 25 ℃ to constant weight to obtain a first component.

Wherein the sodium hydroxide solution in step S11 is sodium hydroxide and deionized water in a ratio of 1 g: 1mL of the sodium hydroxide solution and the chitosan are mixed, and the dosage ratio of the sodium hydroxide solution to the chitosan is 10 g: 10 mL; the dosage ratio of the chitosan, the isopropanol and the chloroacetic acid is 1 g: 12mL of: 1.2 g;

wherein the thionyl chloride mixed solution in the step S12 is thionyl chloride and N, N-dimethylformamide in a volume ratio of 1: 1, mixing; the dosage ratio of the solid a, the N, N-dimethylformamide, the pyridine and the thionyl chloride mixed solution is 1 g: 5mL of: 5mL of: 25 mL;

wherein the phosphate buffer solution in the step S13 is 0.2mol/L Na2HPO4/NaH2PO4 buffer solution, and the volume ratio of the mixed solution A to the mixed solution B to the mixed solution C is 5: 5: 2.

step S21, adding dimethylacetamide into a three-neck flask, adding L-ascorbic acid and dichloromethane under the conditions that the temperature is 0 ℃ and the rotating speed is 200r/min, stirring for 8min, adding solid b for three times in different quantities, keeping the temperature and the rotating speed constant, continuing stirring for 55min, washing the obtained reaction solution with ethyl acetate at 0 ℃, washing with ice water until the filtrate does not contain chloride ions, after washing, drying in vacuum at 25 ℃ to constant weight to obtain a second component, and mixing the first component and the second component according to a mass ratio of 1: 1 mixing to obtain the moisture-preserving antioxidant.

Wherein the dosage ratio of the dimethylacetamide, the L-ascorbic acid, the dichloromethane and the solid b in the step S21 is 6 mL: 1 g: 2.5 mL: 1g of the total weight of the composition.

Example 2

The plant extract is prepared by the following steps:

step A11, weighing the following raw materials in parts by weight: 55 parts of strawberry leaves, 15 parts of angelica, 18 parts of pseudo-ginseng, 13 parts of motherwort, 15 parts of radix ophiopogonis and 2 parts of liquorice;

step A12, mixing the weighed raw materials, adding the mixture into a water-alcohol mixed solution, heating to 82 ℃ and extracting for 65 min;

and step A13, stopping heating, cooling to 35 ℃, filtering, concentrating under reduced pressure, and removing the solvent to obtain the plant extract.

Wherein, the water-alcohol mixed solution in the step A12 is deionized water and propylene glycol according to the volume ratio of 2: 0.8, mixing; the ratio of the total amount of the raw materials to the amount of the water-alcohol mixed solution is 0.8 g: 25 mL.

Example 3

0.6 part of pinoresinol diglucoside, 1 part of dipeptide diaminobutyrylbenzylamide diacetate, 2 parts of sodium hyaluronate, 100 parts of deionized water, 3 parts of glycerol, 10 parts of aloe gel, 50 parts of plant extract, 2 parts of caprylyl glycol, 1 part of 1, 2-hexanediol, 1.6 parts of palmitoyl tripeptide and 3 parts of a moisturizing antioxidant;

firstly, adding pinoresinol diglucoside, dipeptide diaminobutyryl benzylamide diacetate and sodium hyaluronate into deionized water, uniformly mixing, heating to 80 ℃, preserving heat, stirring and uniformly dispersing; regulating the pH value of the solution to 5.5 by using triethanolamine to obtain a first mixture;

Wherein the moisturizing antioxidant was prepared as in example 1; the plant extract was prepared as in example 2.

Example 4

0.7 part of pinoresinol diglucoside, 2 parts of dipeptide diaminobutyrylbenzylamide diacetate, 3 parts of sodium hyaluronate, 150 parts of deionized water, 4 parts of glycerol, 11 parts of aloe vera gel, 60 parts of plant extract, 3 parts of caprylyl glycol, 2 parts of 1, 2-hexanediol, 1.8 parts of palmitoyl tripeptide and 4 parts of moisturizing antioxidant;

firstly, adding pinoresinol diglucoside, dipeptide diaminobutyryl benzylamide diacetate and sodium hyaluronate into deionized water, uniformly mixing, heating to 82 ℃, preserving heat, stirring and uniformly dispersing; regulating the pH value of the solution to 5.5 by using triethanolamine to obtain a first mixture;

Example 5

0.8 part of pinoresinol diglucoside, 3 parts of dipeptide diaminobutyrylbenzylamide diacetate, 4 parts of sodium hyaluronate, 200 parts of deionized water, 5 parts of glycerol, 12 parts of aloe gel, 70 parts of plant extract, 4 parts of caprylyl glycol, 3 parts of 1, 2-hexanediol, 2 parts of palmitoyl tripeptide and 6 parts of a moisturizing antioxidant;

firstly, adding 0.8 part of pinoresinol diglucoside, dipeptide diaminobutyrylbenzyl amide diacetate and sodium hyaluronate into deionized water, uniformly mixing, heating to 85 ℃, preserving heat, stirring and uniformly dispersing; regulating the pH value of the solution to 5.5 by using triethanolamine to obtain a first mixture;

Comparative example 1

The moisture-retaining antioxidant was not added, and the remaining raw materials and preparation process were the same as in example 3.

Comparative example 2

The plant extracts were not added, and the rest of the raw materials and the preparation process were the same as in example 3.

The cosmetic compositions of examples 1 to 3 and comparative examples 1 to 2 were tested;

testing the water content: 50 volunteers, divided into 5 groups, marked 4X 4cm inside the forearm of the left and right hand²Test area, same arm mark 2 areas simultaneously, the interval of area is 2 cm. The test and control areas were measured using a skin moisture test probe assay, 3 replicates per area. The blank value of each test area was measured and then measured at 2.0. + -. 0.1mg sample/cm²The amount of (a) was applied to the test area uniformly using a latex finger cuff. Applying for 14 days and 28 days, respectively, and measuring the skin moisture content of the tested area relative to the blank control area by using a skin moisture test probe;

thin test of desalination: the using method comprises the following steps: the external application is carried out for 2 times every day, the application amount is that the face is evenly coated, and the fine line condition is observed. 50 volunteers, divided into 5 groups; the results are shown in table 1 below:

TABLE 1

As can be seen from table 1 above, the cosmetic composition for stimulating collagen production and resisting wrinkles prepared by the present invention has excellent moisturizing performance and a significant effect of reducing fine lines.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing is illustrative and explanatory only and is not intended to be exhaustive or to limit the invention to the precise embodiments described, and various modifications, additions, and substitutions may be made by those skilled in the art without departing from the scope of the invention or exceeding the scope of the claims.

Claims

1. A cosmetic composition for stimulating collagen to generate and resisting wrinkles is characterized by comprising the following raw materials in parts by weight:

the moisturizing antioxidant is prepared from a first component and a second component in a mass ratio of 1: 1, mixing;

the first component is prepared by the following steps:

step S11, mixing a sodium hydroxide solution and chitosan, standing for 10-12h at the temperature of-18 ℃ after mixing, then unfreezing and transferring to a three-neck flask, adding isopropanol into the three-neck flask, stirring for 30-40min at the temperature of 25 ℃, adding chloroacetic acid in an equivalent manner for three times, with an interval of 5min each time, stirring and reacting for 2h at the temperature of 70 ℃, adding equal volume of distilled water into the solution after the reaction is finished, diluting the solution, adjusting the pH value to 7.0 by glacial acetic acid, carrying out vacuum filtration, washing a filter cake by an ethanol aqueous solution with a volume fraction of 70% and absolute ethanol in sequence, and drying in a 60 ℃ oven after the washing is finished to obtain a solid a;

step S12, adding the solid a and N, N-dimethylformamide into a round-bottom flask, adding pyridine, continuously stirring for 30min at the temperature of 0-5 ℃, dropwise adding thionyl chloride mixed solution by using a constant-pressure dropping funnel, heating to 75 ℃ after dropwise adding, continuously stirring for reacting for 3h, mixing the obtained reaction solution with anhydrous ether with the same volume after the reaction is finished, separating out a precipitate, carrying out vacuum filtration, washing the obtained filter cake with acetone until the filtrate does not contain chloride ions, and after the washing is finished, carrying out vacuum drying at 50 ℃ to constant weight to obtain a solid b;

2. The cosmetic composition for stimulating collagen production to combat wrinkles as claimed in claim 1 wherein the second component is prepared by the steps of:

step S21, adding dimethylacetamide into a three-neck flask, adding L-ascorbic acid and dichloromethane at the temperature of 0 ℃, stirring for 6-10min, adding solid b in different quantities for three times, keeping the temperature and the rotating speed unchanged, continuously stirring for 50-60min, washing the obtained reaction solution with ethyl acetate at the temperature of 0 ℃, washing with ice water until the filtrate does not contain chloride ions, and after washing, drying in vacuum at the temperature of 25 ℃ to constant weight to obtain a second component.

3. The cosmetic composition for stimulating collagen production to combat wrinkles as claimed in claim 1, wherein the plant extract is prepared by the steps of: