CN113079946A - Edible fungus cultivation method and culture medium - Google Patents
Edible fungus cultivation method and culture medium Download PDFInfo
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- CN113079946A CN113079946A CN202110611054.1A CN202110611054A CN113079946A CN 113079946 A CN113079946 A CN 113079946A CN 202110611054 A CN202110611054 A CN 202110611054A CN 113079946 A CN113079946 A CN 113079946A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention discloses an edible fungus cultivation method and a culture medium, and belongs to the technical field of edible fungus cultivation. The method for cultivating the edible fungi comprises the following steps: (1) pretreatment: preparing a culture medium for edible fungus cultivation for later use; (2) and (3) inoculation treatment: inoculating edible fungi into a culture medium; (3) spawn running culture: transferring the culture substrate to a spawn running room for culture; (4) fruiting and culturing: transferring the culture medium to a fruiting chamber for culturing. The culture medium comprises the following raw materials in parts by weight: wood chip: 25-35 parts of bran: 25-35 parts of corncobs: 20-30 parts of soybean meal: 2-10 parts of rice bran: 2-5 parts of light calcium carbonate: 1-2 parts, calcium superphosphate: 1-2 parts, oregano oil: 0.2-0.6 parts of osthole: 0.05 to 0.08 portion. Tests prove that the two index data of the dry quality of the hyphae and the density of the fungus balls have better significant difference, and provide balanced nutrition for the growth of the pleurotus eryngii.
Description
Technical Field
The invention belongs to the technical field of edible fungus culture, and particularly relates to an edible fungus culture method and culture medium.
Background
Pleurotus eryngii (Pleurotus eryngii)Pleurotus eryngii) Pleurotus eryngii belonging to Pleurotus genus of Pleurotaceae family of Agaricales of Basidiomycetes of Eumycota of Pleurotaceae family of Eumycota of Pleurotus eryngii belonging to rare edible fungi. The pleurotus eryngii fruiting body is white in color, thick in meat and crisp in texture, has unique almond flavor and abalone flavor, and has the reputation of king in mushroom. Pleurotus eryngii is rich in proteins, essential amino acids, dietary fibers and various trace elements, and contains a large amount of bioactive substances such as polysaccharides, terpenoids, flavonoids and the like. Has anticancer, blood lipid reducing, and blood lipid reducing effectsHas multiple effects of promoting gastrointestinal digestion, preventing cardiovascular diseases and the like, and is a precious medicinal fungus.
At present, pleurotus eryngii is mainly produced in a traditional mode, usually, a cultivation material and pleurotus eryngii spores are subjected to bag cultivation, bottle cultivation or box cultivation, and the pleurotus eryngii grows in a relatively closed space, so that air circulation is poor and water is not uniformly supplied in the growth process of hypha and thalli, so that the yield is unstable, the space utilization rate is low, the survival rate of strains is low due to the influence of seasons, facilities and environmental conditions caused by nutrient imbalance, the hypha is easy to age, strain variation is caused, the growth of the pleurotus eryngii in the later period is influenced, the nutrition structure is unreasonable, the yield of the pleurotus eryngii is reduced, the quality is reduced, and other serious consequences are caused.
Disclosure of Invention
Problems to be solved
Aiming at the problems in the prior art, the invention provides the edible fungus cultivation method and the culture medium, so that the two index data of the dry quality of the hyphae and the density of the fungus balls have better significant difference, balanced nutrition is provided for the growth of the pleurotus eryngii, the growth speed of the pleurotus eryngii is high, the growth period is short, and the time to market is accelerated.
Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
A culture medium for the method for cultivating edible fungi,
the composite material comprises the following raw materials in parts by weight:
25 to 35 parts of wood chips,
25 to 35 portions of bran coat,
20 to 30 parts of corncobs,
2 to 10 portions of soybean meal,
2 to 5 parts of rice bran,
1 to 2 portions of light calcium carbonate,
1 to 2 parts of calcium superphosphate,
0.2 to 0.6 portion of oregano oil,
0.05 to 0.08 portion of osthole.
In the culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
27 to 32 parts of wood chips,
28 to 33 portions of bran coat,
23 to 27 parts of corncobs,
4-8 parts of soybean meal,
2 to 5 parts of rice bran,
1 to 2 portions of light calcium carbonate,
1 to 2 parts of calcium superphosphate,
0.2 to 0.6 portion of oregano oil,
0.05 to 0.08 portion of osthole.
In the culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
30 parts of wood chips, namely wood chips,
30 parts of bran, namely wheat bran,
25 parts of corncobs, namely corn cobs,
6 parts of soybean meal, namely 6 parts of soybean meal,
4 parts of rice bran, namely, rice bran 4 parts,
2 parts of light calcium carbonate, namely 2 parts of light calcium carbonate,
2 parts of calcium superphosphate, namely calcium superphosphate,
0.4 part of oregano oil,
0.07 part of osthole.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, light calcium carbonate, calcium superphosphate, origanum oil and osthole into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
in the invention, the two index data of the dry quality of the hyphae and the density of the fungus balls have better significant difference. On the basis of the traditional technology, the light calcium carbonate, the calcium superphosphate, the osthole and the oregano oil are creatively introduced into the components of the culture medium, and the four substances, particularly the osthole and the oregano oil, play a key role in improving the parameter values of the mycelium dry mass and the pellet density of the pleurotus eryngii.
Detailed Description
The invention is further described with reference to specific examples.
Example 1
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
25 parts of wood chips, namely 25 parts of wood chips,
35 parts of wheat bran, namely, wheat bran,
20 parts of corn cob, namely 20 parts of corn cob,
10 parts of soybean meal, namely 10 parts of soybean meal,
2 parts of rice bran, namely 2 parts of rice bran,
2 parts of light calcium carbonate, namely 2 parts of light calcium carbonate,
1 part of calcium superphosphate, namely calcium superphosphate,
0.6 part of oregano oil,
0.05 part of osthole.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, light calcium carbonate, calcium superphosphate, origanum oil and osthole into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Example 2
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
35 parts of wood chips, namely wood chips,
25 parts of bran, namely, 25 parts of wheat bran,
30 parts of corn cob, namely 30 parts of corn cob,
2 parts of soybean meal, namely 2 parts of soybean meal,
5 parts of rice bran, namely 5 parts of rice bran,
1 part of light calcium carbonate, namely 1 part of light calcium carbonate,
2 parts of calcium superphosphate, namely calcium superphosphate,
0.2 part of oregano oil,
0.08 portion of osthole.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, light calcium carbonate, calcium superphosphate, origanum oil and osthole into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Example 3
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
27 parts of wood chips, namely wood chips,
33 parts of bran, namely, wheat bran,
23 parts of corn cob, namely,
8 parts of soybean meal, namely 8 parts of soybean meal,
2 parts of rice bran, namely 2 parts of rice bran,
2 parts of light calcium carbonate, namely 2 parts of light calcium carbonate,
1 part of calcium superphosphate, namely calcium superphosphate,
0.6 part of oregano oil,
0.05 part of osthole.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, light calcium carbonate, calcium superphosphate, origanum oil and osthole into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Example 4
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
32 parts of wood chips, namely 32 parts of wood chips,
28 parts of bran, namely 28 parts of wheat bran,
27 parts of corn cob, namely corn cob,
4 parts of soybean meal, namely 4 parts of soybean meal,
5 parts of rice bran, namely 5 parts of rice bran,
1 part of light calcium carbonate, namely 1 part of light calcium carbonate,
2 parts of calcium superphosphate, namely calcium superphosphate,
0.2 part of oregano oil,
0.08 portion of osthole.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, light calcium carbonate, calcium superphosphate, origanum oil and osthole into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Example 5
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
30 parts of wood chips, namely wood chips,
30 parts of bran, namely wheat bran,
25 parts of corncobs, namely corn cobs,
6 parts of soybean meal, namely 6 parts of soybean meal,
4 parts of rice bran, namely, rice bran 4 parts,
2 parts of light calcium carbonate, namely 2 parts of light calcium carbonate,
2 parts of calcium superphosphate, namely calcium superphosphate,
0.4 part of oregano oil,
0.07 part of osthole.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, light calcium carbonate, calcium superphosphate, origanum oil and osthole into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Comparative example 1
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
30 parts of wood chips, namely wood chips,
30 parts of bran, namely wheat bran,
25 parts of corncobs, namely corn cobs,
6 parts of soybean meal, namely 6 parts of soybean meal,
4 parts of rice bran, namely, rice bran 4 parts,
0.4 part of oregano oil,
0.07 part of osthole.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, oregano oil and osthole into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Comparative example 2
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
30 parts of wood chips, namely wood chips,
30 parts of bran, namely wheat bran,
25 parts of corncobs, namely corn cobs,
6 parts of soybean meal, namely 6 parts of soybean meal,
4 parts of rice bran, namely, rice bran 4 parts,
2 parts of light calcium carbonate, namely 2 parts of light calcium carbonate,
2 parts of calcium superphosphate, namely calcium superphosphate,
0.4 part of oregano oil.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding wood dust, bran, corncob, bean pulp, rice bran, light calcium carbonate, calcium superphosphate and oregano oil into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Comparative example 3
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
30 parts of wood chips, namely wood chips,
30 parts of bran, namely wheat bran,
25 parts of corncobs, namely corn cobs,
6 parts of soybean meal, namely 6 parts of soybean meal,
4 parts of rice bran, namely, rice bran 4 parts,
2 parts of light calcium carbonate, namely 2 parts of light calcium carbonate,
2 parts of calcium superphosphate, namely calcium superphosphate,
0.07 part of osthole.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, light calcium carbonate, calcium superphosphate and osthole into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Comparative example 4
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
30 parts of wood chips, namely wood chips,
30 parts of bran, namely wheat bran,
25 parts of corncobs, namely corn cobs,
6 parts of soybean meal, namely 6 parts of soybean meal,
4 parts of rice bran, namely, rice bran 4 parts,
2 parts of light calcium carbonate, namely 2 parts of light calcium carbonate,
and 2 parts of calcium superphosphate.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, light calcium carbonate and calcium superphosphate into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Comparative example 5
The method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
In the above-mentioned method for cultivating edible fungi,
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
In the above-mentioned method for cultivating edible fungi,
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
In the above-mentioned method for cultivating edible fungi,
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
In the above-mentioned method for cultivating edible fungi,
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
In the above-mentioned method for cultivating edible fungi,
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
The culture medium of the edible fungus cultivation method,
the composite material comprises the following raw materials in parts by weight:
30 parts of wood chips, namely wood chips,
30 parts of bran, namely wheat bran,
25 parts of corncobs, namely corn cobs,
6 parts of soybean meal, namely 6 parts of soybean meal,
4 parts of rice bran, namely, rice bran 4 parts,
and 2 parts of calcium superphosphate.
In the culture medium of the edible fungus cultivation method,
the preparation method of the culture medium comprises the following steps:
adding wood dust, bran, corncobs, bean pulp, rice bran and calcium superphosphate into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
Comparative example 6
Chinese invention patent, application number: CN201210327369.4, publication No.: CN102845219B discloses a pleurotus eryngii cultivation process, the specification of which is as follows:
the technical idea of the pleurotus eryngii cultivation process is as follows:
1) firstly, screening and propagating a culture medium suitable for pleurotus eryngii strains and a proportion. Research shows that the culture medium for expanding propagation of strains is prepared by using wheat grains and wood chips as main raw materials, the obtained strains have strong vitality, after the culture medium is mixed with the sterilized culture medium, the strains are distributed with more points, germinate early and grow fast, and can grow in the culture medium and on the surface of the culture medium;
2) the cultivation substrate for producing the pleurotus eryngii is sterilized, inoculated and then subpackaged, and is sterilized for a short time (8 minutes) at the ultrahigh temperature (138 ℃), so that the sterilization time is shortened, the cooling, seed dressing and subpackaging of the cultivation substrate are all carried out in an aseptic environment, and the working efficiency and the equipment utilization rate are improved;
3) uniformly stirring strains in a stirring mode; can greatly shorten the spawn running period, shorten the production period and reduce the production cost.
The following is an example given by the inventors, and it should be noted that the present invention is not limited to this example.
The embodiment provides a pleurotus eryngii production process, which comprises the following steps:
1) preparing strains: under the aseptic condition, transferring the pleurotus eryngii strain to a comprehensive PDA slant culture medium, and culturing for 15 days at 26 ℃ to obtain a first-grade pleurotus eryngii strain.
The culture medium for preparing the second-level strain and the third-level strain adopts the following formula: 60% of wheat grains, 39.35% of broad-leaved tree wood chips, 0.5% of edible sucrose, 0.1% of monopotassium phosphate and 0.05% of magnesium sulfate.
The preparation method of the culture medium comprises the following steps:
(1) rinsing the wheat: and (3) rinsing the used wheat with clear water, and removing floating impurities and hollow particles.
(2) Soaking or cooking: soaking wheat or steaming wheat grains at 60 deg.C until the wheat grains absorb water until there is no white core (water content is about 65%);
(3) preparing a nutrient solution: dissolving edible sucrose, potassium dihydrogen phosphate and magnesium sulfate with water to obtain a nutrient solution;
(4) uniformly mixing and stirring the wheat grains, the broad-leaved tree wood chips and the nutrient solution which are expanded by water absorption, and adjusting the mixture to have the water content of 65 percent to obtain a culture medium;
(5) subpackaging, sterilizing and cooling: subpackaging the culture medium into strain bottles or strain bags, sterilizing at 121 deg.C for 2h, and cooling;
(6) inoculation: inoculating the strain on a culture medium under aseptic condition, inoculating the first-stage strain to propagate the second-stage strain, and inoculating the second-stage strain to propagate the third-stage strain.
2) Production process flow
(1) Preparing and sterilizing a culture medium:
the culture medium can be prepared by itself or commercialized.
The culture medium of this example uses the following weight percentage formulation (on a dry basis): broad-leaved tree sawdust 34%, cottonseed hull 20%, corncob 20%, bran 15%, soybean meal 7%, corn flour 3% and gypsum 1%.
The preparation method of the culture medium comprises weighing raw materials in the formula, adding water, stirring to reach required water content (about 65%), adjusting pH to 7.5, stirring, directly packaging into large package (non-culture bag or culture bottle) or directly packaging into a sterilizing pot without packaging, and sterilizing at 138 deg.C for 8 min.
(2) And (3) cooling: the sterilized culture medium is cooled to below 22 deg.C in sterile environment, and can be vacuumized, cold water heat-transfer and cold-air forced cooling.
(3) Mixing strains: in a sterile environment, dispersing the third-level strain of the pleurotus eryngii into small particles, and preferably dispersing the wheat particles independently. Uniformly mixing the dispersed three-level strains into the sterilized and cooled culture medium by a stirring mode at one time according to the mass ratio of 10%, because the strains on the wheat grains have strong vitality, the strains can quickly grow by utilizing oxygen in gaps in the culture medium, mycelium of the strains in the whole bag or bottle is connected, and then the oxygen in the culture chamber is utilized to grow through the bag surface or the bottle opening.
(4) Subpackaging: in a sterile room, the culture medium with the pleurotus eryngii strains is subpackaged in conventional culture bags or culture bottles, and is transferred to a mycelium culture room after being sealed by a sterile lantern ring and a sealing cover. Culturing at room temperature of 22 deg.C and relative air humidity of 70% for 10 days under good ventilation condition, allowing mycelia of Pleurotus eryngii to fill the culture bag or bottle, culturing for 10 days to reduce temperature, and performing normal mushroom management.
(5) Management: differentiation and growth of pleurotus eryngii fruiting bodies and fruiting of pleurotus eryngii are managed according to a conventional process.
Comparative example 7
Chinese invention patent, application number: CN201510366848.0, publication No.: CN104987151A discloses a culture medium for pleurotus eryngii and a culture method for pleurotus eryngii, and the specification of the culture medium is as follows:
the culture medium for the pleurotus eryngii comprises the following raw materials in percentage by weight: 13% of tea seed hulls, 11% of lotus seed hulls, 10% of hazelnut hulls, 11% of hemp hulls, 24% of cotton seed hulls, 7% of wheat bran, 4% of wheat flour, 4% of sorghum flour, 2% of soybean flour, 4% of tea bran, 4% of hemp bran, 2% of bovine bone meal, 3% of lime powder and 1% of brown sugar.
A pleurotus eryngii cultivation method comprises the following steps:
(1) material preparation and fermentation: crushing tea seed hulls, lotus seed hulls, hazelnut hulls, hemp hulls and cottonseed hulls, crushing the crushed tea seed hulls, lotus seed hulls, hazelnut hulls, hemp hulls and cottonseed hulls into particles with the particle size of 7mm, adding water to the crushed tea seed hulls, stirring the crushed tea seed hulls, the water to be added while stirring, wherein the humidity is suitable for the situation that water seeps out but does not drip out when the culture material is held by hands, then piling the mixture for fermentation for 3 days, and turning the pile every day at the fermentation temperature of about 60 ℃; mixing testa Tritici, semen Tritici Aestivi powder, jowar powder, semen glycines powder, tea bran, fructus Cannabis bran, Os bovis Seu Bubali powder, calx powder, and brown sugar with water to obtain paste, standing for 4 hr, mixing all materials, and fermenting for 2 days at 60 deg.C.
(2) Bagging, namely bagging the fermented material by using a bagging machine when the water content of the fermented material is about 63 percent, wherein a film tube material with the diameter of 18 cm and a tube bag with the length of 45 cm are used for bagging.
(3) And (3) sterilizing, namely, folding the bag openings of the material bags filled with the culture materials in order at two ends, then putting the material bags into the woven bag, and then putting the material bags into a sterilization pot, so that the material bags are convenient to take out of the pot and transport the sterilized material bags. When the sterilization temperature reaches 100 ℃, keeping for 20 hours, then stopping heating, and taking out the material bag after 12 hours of stewing.
(4) Inoculating, namely moving the sterilized material bag into an inoculating room which is sterilized in advance, continuously sterilizing the material bag in the inoculating room again by using an edible fungus smoke disinfectant, and spraying 84 disinfectant in the air for sterilization when people enter the inoculating room; after sterilization, taking out the material bag from the woven bag, respectively putting a layer of strain with the thickness of 3cm at two ends of the material bag (namely a two-end inoculation method), and folding the opening of the bag in half; one person is responsible for putting the inoculated fungus cylinders into another clean and disinfected woven bag; conveniently move into and cultivate the room, and the braided bag can block the invasion fungus section of thick bamboo of miscellaneous fungus, reduces a fungus section of thick bamboo and infects, has both made things convenient for the transportation to have improved the probability that a fungus section of thick bamboo cultivateed successfully.
(5) Spawn running management: and (3) placing the inoculated fungus sticks into a culture room in a dark environment for culture, controlling the indoor temperature to be 28 ℃, ventilating frequently, and keeping the air fresh, wherein the humidity cannot exceed 70%.
(6) The fruiting management comprises the following steps:
a) stacking mushroom sticks, namely stacking the mushroom sticks into a cuboid with the length of 1m, the width of 0.8m and the height of 1m or a cube with the length of 1m, the width of 1m and the height of 1m in a mushroom shed by using soil, wherein the length of each mushroom stick is 0.3m, when the cube or the cuboid is stacked by using the mushroom sticks, firstly stacking the front face, the rear face, the left face and the right face, then filling prepared soil in the middle, and finally stacking the top face; before building the fungus sticks, the fungus sticks must be full of fungus and the outer bag must be removed. After the whole cube is built, a plurality of (three) water dropping pipes are inserted into the middle of the nutrient mud, the water dropping pipes are PVC pipes, and round holes with the diameter of 3cm are drilled at the positions of every 10cm by using electric drills. The nutrient solution is used for supplementing nutrient solution to the whole cubic fungus stick in the later period of fruiting, thereby improving the yield. The soil-building fruiting mode enables the mushroom sticks to well absorb nutrients in soil, and artificial nutrient solution is injected through the PVC pipes, so that the mushroom sticks can grow more mushrooms, the purpose of high yield is achieved, and the occupied area is greatly reduced. Also avoids the defect that the stacked mushrooms are easy to burn.
b) Inducing bud, filling water to the ground after the hypha is full, the humidity is 90%, promoting bud formation, enhancing ventilation, and the temperature difference is 10 ℃. After the mushroom body appears, reasonable environmental conditions are created. Keeping the ground moist, spraying into the air with the air humidity of about 85%, and ventilating for 30 minutes in time after each water spraying. When the mushroom grows to be more than 2 cm, water can be sprayed to the mushroom. Keeping proper fruiting temperature, and performing heat preservation or aeration cooling.
(7) And (4) harvesting, namely, when the cap of the pleurotus eryngii is flat and spores are not ejected yet, the period is the optimum period for harvesting. Collecting standard; according to the market demand, the pleurotus eryngii stem is 8 cm long, 4 cm thick and smaller than the pileus. After the first tide of mushrooms is picked, mushroom sticks are cleaned, and dead mushrooms and residual roots are cleaned. And (3) stopping water for 3 days after harvesting every tide, culturing the mushrooms for 10 days, and then injecting nutrient solution into the water dripping pipe in the middle of the mud column for secondary fruiting management until few mushrooms are produced.
Preferably, the soil in the step (6) comprises the following components in percentage by weight: 2% of lime, 2% of coconut husk, 15% of bagasse, 40% of granular fertile soil and 41% of mushroom bran.
Preferably, in the fruiting management process in the step (6), a nutrient solution is injected into the mushroom sticks, and the nutrient solution comprises the following components in percentage by weight: 1% of brown sugar, 5% of potato boiled water, 1% of lime, 0.04% of magnesium sulfate, 0.04% of monopotassium phosphate and the balance of water. The boiled water of potatoes is obtained by adding 100g of sliced potatoes into 1000g of water, boiling and filtering.
Comparative example 8
Chinese invention patent, application number: CN201610721056.5, publication No.: CN106242824B discloses a pleurotus eryngii cultivation material and a planting method for producing pleurotus eryngii by using the cultivation material, and the specification of the cultivation material is as follows:
"wherein the wood chips in example 2 are elm wood chips and pine wood chips, and the straws are wheat straws and corn straws, wherein the weight ratio of the elm wood chips to the pine wood chips is 1:1, and the weight ratio of the wheat straws to the corn straws is 1: 2.
The planting method of the pleurotus eryngii comprises the following steps:
(1) semi-fermented cultivation material: crushing and uniformly mixing the cultivation material in the embodiment 2, then adding water to ensure that the water content of a culture medium is 60%, adding a fermentation strain to perform semi-fermentation to obtain a fermentation material, wherein the semi-fermentation condition is that the temperature is 27 ℃ and the time is 8 hours;
(2) manufacturing a cultivation bag: curling the sponge into a barrel shape or a spiral shape, sewing the curled sponge by using cotton threads, sealing one end of the curled sponge, inserting cotton into the sealed position, adding the prepared fermentation material into the sponge, then inserting the cotton into the other end of the sponge by using the same method, and sealing the sealed position, wherein the weight of the whole cultivation bag is controlled to be 3kg, and the sponge for manufacturing the cultivation bag has the length of 50cm, the width of 50cm and the thickness of 0.7 cm;
(3) and (3) sterilization and cooling: pasteurizing the prepared cultivation bag, and cooling to room temperature of 25 ℃;
(4) inoculating and culturing: inoculating pleurotus eryngii strains, and culturing in dark place; the cultivation was carried out at 24 ℃ under dark conditions and a humidity of 65% for 30 days, at a pH of 6.8. Then, the culture was carried out under light at 14 ℃ and 87% humidity for 17 days under light of 650 lx. After 17 days, removing the seal and taking out the cotton;
(5) harvesting head tide mushrooms: harvesting when the length of the fruiting body of the pleurotus eryngii is 13cm and the cap of the pleurotus eryngii is 6 cm;
(6) harvesting two-tide mushrooms: sterilizing the culture bag, supplementing water and nutrient solution, ventilating, performing illumination culture, and harvesting after growing the second tide of mushrooms.
The nutrient solution comprises the following components in parts by weight:
urea: 0.2%, potassium dihydrogen phosphate: 0.2%, yeast extract: 0.2%, pig manure: 0.3%, gypsum: 0.1% and the balance water ".
The pleurotus eryngii cultivated in the above examples 1 to 5 and the pleurotus eryngii cultivated in the comparative examples 1 to 8 were subjected to the relevant tests with reference to the following prior arts:
the first testing technology comprises the following steps: zhangjie, Lu Dan, Shibin, response surface experiments optimized culture conditions for Pleurotus eryngii liquid fermentation strain [ J ] food science, 2017, 38(006): 147-.
And (2) testing technology II: liulina, Wang Anjian, Wei Shuxin, and the like, research on the production of gamma-aminobutyric acid by fermenting mushroom stem zymolyte by lactic acid bacteria [ J ] packaging and food machinery, 2018(4) and 13-19.
And (3) measuring the yield of the pleurotus eryngii:
and (3) measuring the density and the dry mass of the mycelium pellet, namely taking 100mL of fermentation liquor, filtering with eight layers of gauze to obtain the mycelium pellet, and drying in a 105 ℃ forced air drying oven to obtain the dry mass (g/100 mL) of the mycelium. Taking 1mL of fermentation liquor, diluting by 10 times, taking 1mL of diluted fermentation liquor, and counting the number of bacteria balls, wherein the density of the bacteria balls can be obtained by the following formula:
cell density/(piece/L) = AxB x103
In the formula: a is the volume/mL of the fermentation liquor stock solution; b is the dilution factor of the fermentation liquor; 103The volume was 1000 mL.
The test shows that the dry mass of the hyphae in example 1 is 1.24g/100mL, and the density of the mycelium pellet is 6.5x104Per liter;
in example 2, the dry mass of the mycelia was 1.26g/100mL, and the pellet density was 6.6X104Per liter;
in example 3, the dry mass of the mycelia was 1.25g/100mL,the density of the bacteria pellet is 6.6x104Per liter;
in example 4, the dry mass of the mycelia was 1.28g/100mL, and the pellet density was 6.7X104Per liter;
in example 5, the dry mass of the mycelia was 1.30g/100mL, and the pellet density was 6.9X104Per liter;
in comparative example 1, the dry mass of the mycelia was 1.21g/100mL, and the pellet density was 6.1X104Per liter;
in comparative example 2, the dry mass of the mycelia was 1.14g/100mL, and the pellet density was 5.8X104Per liter;
in comparative example 3, the dry mass of the mycelia was 1.08g/100mL, and the pellet density was 5.3X104Per liter;
in comparative example 4, the dry mass of the mycelia was 1.09g/100mL, and the pellet density was 5.3X104Per liter;
in comparative example 5, the dry mass of mycelia was 1.01g/100mL, and the pellet density was 4.8X104Per liter;
in comparative example 6, the dry mass of the mycelia was 1.05g/100mL, and the pellet density was 5.0X104Per liter;
in comparative example 7, the dry mass of the mycelia was 1.20g/100mL, and the pellet density was 6.1X104Per liter;
in comparative example 8, the dry mass of mycelia was 1.26g/100mL, and the pellet density was 6.6X104Per liter;
the data show that compared with comparative examples 1-8, the two index data of the dry mass of the hyphae and the density of the fungus balls show significant difference effects in examples 1-5, and further, the data of comparative examples 1-5 show that light calcium carbonate, calcium superphosphate, osthole and oregano oil play a critical role.
While the invention has been described in further detail in connection with specific embodiments thereof, it will be understood that the invention is not limited thereto, and that various other modifications and substitutions may be made by those skilled in the art without departing from the spirit of the invention, which should be considered to be within the scope of the invention as defined by the appended claims.
Claims (10)
1. A method for cultivating edible fungi comprises the following steps:
(1) pretreatment: preparing a culture medium for edible fungus cultivation for later use;
(2) and (3) inoculation treatment: inoculating edible fungi into the culture medium in the step (1);
(3) spawn running culture: transferring the culture substrate inoculated in the step (2) to a bacteria growing room for culture;
(4) fruiting and culturing: and (4) transferring the culture medium subjected to spawn running culture in the step (3) to a fruiting room for culture.
2. The method for cultivating edible fungi according to claim 1, wherein:
the edible fungus in the step (1) is pleurotus eryngii with the scientific name of pleurotus eryngiiPleurotus eryngii。
3. The method for cultivating edible fungi according to claim 1, wherein:
the inoculation amount of the edible fungi in the step (2) is 5% of the weight of the culture medium.
4. The method for cultivating edible fungi according to claim 1, wherein:
the temperature of the spawning chamber in the step (3) is 25 ℃;
the relative humidity of the air in the fermentation chamber in the step (3) is 65 percent.
5. The method for cultivating edible fungi according to claim 1, wherein:
and (4) keeping the bacteria growing room in the step (3) in a dark environment condition.
6. The method for cultivating edible fungi according to claim 1, wherein:
the concentration of carbon dioxide in the fruiting chamber in the step (4) is not more than 2000 mg/kg;
the relative air humidity of the fruiting chamber in the step (4) is 85%;
the daily illumination time of the fruiting chamber in the step (4) is 4h, and the illumination intensity is 600 Lux.
7. A culture medium for use in the method of cultivating edible fungi according to claim 1, wherein:
the composite material comprises the following raw materials in parts by weight:
25 to 35 parts of wood chips,
25 to 35 portions of bran coat,
20 to 30 parts of corncobs,
2 to 10 portions of soybean meal,
2 to 5 parts of rice bran,
1 to 2 portions of light calcium carbonate,
1 to 2 parts of calcium superphosphate,
0.2 to 0.6 portion of oregano oil,
0.05 to 0.08 portion of osthole.
8. The culture medium for edible mushroom cultivation method according to claim 7, characterized in that:
the composite material comprises the following raw materials in parts by weight:
27 to 32 parts of wood chips,
28 to 33 portions of bran coat,
23 to 27 parts of corncobs,
4-8 parts of soybean meal,
2 to 5 parts of rice bran,
1 to 2 portions of light calcium carbonate,
1 to 2 parts of calcium superphosphate,
0.2 to 0.6 portion of oregano oil,
0.05 to 0.08 portion of osthole.
9. The culture medium for edible mushroom cultivation method according to claim 8, characterized in that:
the composite material comprises the following raw materials in parts by weight:
30 parts of wood chips, namely wood chips,
30 parts of bran, namely wheat bran,
25 parts of corncobs, namely corn cobs,
6 parts of soybean meal, namely 6 parts of soybean meal,
4 parts of rice bran, namely, rice bran 4 parts,
2 parts of light calcium carbonate, namely 2 parts of light calcium carbonate,
2 parts of calcium superphosphate, namely calcium superphosphate,
0.4 part of oregano oil,
0.07 part of osthole.
10. The culture medium for edible mushroom cultivation method according to claim 9, characterized in that:
the preparation method of the culture medium comprises the following steps:
adding sawdust, bran, corncob, soybean meal, rice bran, light calcium carbonate, calcium superphosphate, origanum oil and osthole into a stirring tank, adding water, stirring, bagging, and performing irradiation sterilization;
wherein the water content in the culture medium is 65%.
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