CN113046239A - Cell culture device - Google Patents
Cell culture device Download PDFInfo
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- CN113046239A CN113046239A CN202110287717.9A CN202110287717A CN113046239A CN 113046239 A CN113046239 A CN 113046239A CN 202110287717 A CN202110287717 A CN 202110287717A CN 113046239 A CN113046239 A CN 113046239A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 39
- 238000004140 cleaning Methods 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 230000005540 biological transmission Effects 0.000 claims description 72
- 239000007788 liquid Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 16
- 238000007790 scraping Methods 0.000 abstract description 13
- 239000007921 spray Substances 0.000 abstract description 11
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 238000005457 optimization Methods 0.000 description 9
- 238000009434 installation Methods 0.000 description 6
- 241000700605 Viruses Species 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000001680 brushing effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/087—Cleaning containers, e.g. tanks by methods involving the use of tools, e.g. brushes, scrapers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/093—Cleaning containers, e.g. tanks by the force of jets or sprays
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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Abstract
The invention belongs to the technical field of cell culture equipment, and particularly relates to a cell culture device which comprises an incubator, wherein a cover is detachably connected to the upper end surface of the incubator, a motor is installed on the upper end surface of the cover, a first bearing is fixedly connected to the lower end surface of the cover, a plurality of spray heads spray water in a water tank to spray and flush the inside of the incubator, the cleaning effect is greatly improved by matching with bristles, a cleaning disc is driven to rotate by starting the motor, two scraping blades are driven to rotate, suspended cells stained on the inner wall side of the incubator are scraped, the cleaning effect is further improved, the inside of the incubator is kept clean, the next cell culture work is facilitated, the problems that the existing cell culture device is generally cleaned manually after being used, the cleaning effect is poor, time and labor are wasted, and the next cell sheet culture effect is influenced if the suspended cells attached to the inner wall of the culture device cannot be cleaned thoroughly are solved, is not beneficial to the cell culture work.
Description
Technical Field
The invention relates to the technical field of cell culture equipment, in particular to a cell culture device.
Background
Cells (English name: cell) are not uniformly defined, and the more common method is: cells are the basic structural and functional units of an organism. It is known that all organisms except viruses are composed of cells, but the vital activities of the viruses must be embodied in the cells, and the cell culture work is widely applied to various fields such as biology, medicine, research and development of new drugs, and becomes one of the most important basic sciences.
The existing cell culture device is usually washed manually after being used, the washing effect is poor, time and labor are wasted, if the suspended cells attached to the inner wall of the culture device cannot be thoroughly cleaned, the next cell sheet culture effect can be influenced, and the cell culture work is not facilitated.
Disclosure of Invention
In order to solve the problems in the background art, the invention provides a cell culture device, which has the characteristics of being capable of automatically cleaning the culture device, good in cleaning effect, time-saving and labor-saving, capable of ensuring the cleaning quality of the interior of an incubator and beneficial to cell culture work.
In order to achieve the purpose, the invention provides the following technical scheme: a cell culture device comprises an incubator, wherein the upper end surface of the incubator is detachably connected with a case cover, the upper end surface of the case cover is provided with a motor, the output end of the motor is fixedly connected with a first rotating shaft, the lower end surface of the case cover is fixedly connected with a first bearing, the lower end surface of the first rotating shaft sequentially penetrates through the case cover and the first bearing and extends to the lower part of the first bearing, the first rotating shaft is rotatably connected with the first bearing, the lower end surface of the first rotating shaft is fixedly connected with an upper transmission disc, the lower end surface of the upper transmission disc is fixedly connected with a plurality of upper transmission shafts distributed in a circular ring shape, the right side wall of the first bearing is fixedly connected with an upper connecting rod, the lower end surface of the upper connecting rod is fixedly connected with a hinge wheel, the outer wall of the hinge wheel is movably connected with a lower connecting rod, the other end of the lower connecting rod is fixedly connected with a second bearing, the inner, the upper end face of the second rotating shaft is fixedly connected with a lower transmission disc, the upper end face of the lower transmission disc is fixedly connected with a plurality of lower transmission shafts distributed in a circular ring shape, the lower transmission shafts are meshed with the upper transmission shafts, the lower end face of the second rotating shaft is fixedly connected with a cleaning disc, bristles are arranged on the lower end face of the cleaning disc, connectors are fixedly connected to the left side and the right side of the cleaning disc, connecting cylinders are detachably connected to the opposite sides of the two connectors, fixed plates are fixedly connected to the opposite sides of the two connecting cylinders, scraping sheets are fixedly connected to the opposite sides of the two fixed plates, the outer walls of the scraping sheets are respectively abutted against the left side and the right side of the inner wall of the incubator, a water tank is arranged on the right side wall of the incubator, oblique blocks are fixedly connected to the left side and the right side of the inner bottom of the incubator, and mounting plates are fixedly connected to the, two a plurality of shower nozzles are all installed to the up end of mounting panel, and are a plurality of the shower nozzle all communicates with the water tank through the pipeline.
In order to facilitate the meshing transmission of the upper transmission disc and the lower transmission disc, it is preferable that the upper transmission disc and the lower transmission disc have the same structural size, and the upper connecting rod and the lower connecting rod have the same structural size.
In order to drive the lower connecting rod to rotate, as a preferable mode of the cell culture device of the present invention, a driving motor is installed on the front end surface of the hinge wheel, and the output end of the driving motor penetrates through the hinge wheel and extends to the back of the hinge wheel to be fixedly connected with the upper connecting rod.
In order to facilitate control of the operation device, it is preferable that the right side wall of the incubator is fixedly connected with two connecting rods, and the right side walls of the two connecting rods are fixedly connected with a control panel.
In order to control the internal temperature of the incubator, it is preferable that a thermostatic control box is provided on the left side wall of the incubator, and the thermostatic control box is electrically connected to the control panel.
In order to detect the internal temperature of the incubator in real time, it is preferable that a temperature sensor is disposed on the right side of the top end of the incubator, and the temperature sensor is electrically connected to the control panel.
In order to facilitate the addition of cells and a culture solution, it is preferable that the left side of the upper end surface of the incubator is provided with a cell inlet and a culture solution inlet, and the cell inlet is positioned on the left side of the culture solution inlet.
In order to add water to the inside of the water tank, it is preferable that the outer wall of the water tank is provided with a liquid level window, and the upper end surface of the water tank is provided with a water inlet.
In order to facilitate the discharge of the cell culture solution, it is preferable that the bottom end of the outer wall of the incubator is provided with a cell outlet, and the outer wall of the cell outlet is provided with a valve.
In order to observe the operation of the inside of the incubator, it is preferable that the outer wall of the incubator is provided with an observation window.
Compared with the prior art, the invention has the beneficial effects that:
after the incubator is used, a motor is started, the motor drives a first rotating shaft to rotate, a first bearing enables the first rotating shaft to rotate more stably, the first rotating shaft drives an upper transmission disc at the lower end and a plurality of upper transmission shafts to rotate, a lower transmission disc is meshed with the upper transmission shafts through a plurality of lower transmission shafts, so that a lower transmission disc is driven to rotate, a driving motor is started simultaneously, the driving motor drives a lower connecting rod to rotate ninety degrees anticlockwise, the lower connecting rod drives a second bearing to rotate ninety degrees anticlockwise, so that the lower transmission shaft and the upper transmission shafts rotate ninety degrees anticlockwise, the upper transmission shafts are vertically meshed with the lower transmission shafts, the lower transmission shafts can still be driven to rotate, a cleaning disc at the lower end of a second rotating shaft is parallel to the right side wall of the incubator, bristles are abutted against the side wall of the incubator, and the motor drives the cleaning disc to rotate when in work, thereby it is clean to rotate the lateral wall to the incubator, start a plurality of shower nozzles of bottom simultaneously, a plurality of shower nozzles are with the inside water blowout of water tank, thereby spray and wash to incubator inside, the cooperation brush hair improves clean effect greatly, tentatively clean the back that finishes, start driving motor once more and drive down connecting rod clockwise ninety degrees, thereby make down driving disk and lower transmission shaft all playback, clean dish is the vertical state with the incubator lateral wall this moment, then cup joint the doctor-bar in the connector through the connecting cylinder and accomplish the installation, two doctor-bars after the installation respectively with the both sides wall butt of incubator, the starter motor then drives clean dish and rotates, thereby drive two doctor-bars and rotate, be stained with the suspended cells who covers with incubator inner wall side and strike off, further improve clean effect, thereby make the incubator inside keep clean, be favorable to next cell culture work.
To sum up, this kind of cell culture device has can wash culture apparatus automatically, and the cleaning performance is good, and labour saving and time saving guarantees the inside clean quality of incubator, is favorable to the characteristics of cell culture work.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is an overall internal structural view of the present invention;
FIG. 2 is a schematic view of the structure of the upper connecting rod of the present invention;
FIG. 3 is a bottom view of the upper tray of the present invention;
FIG. 4 is a schematic view of the folded state of the lower connecting rod of the present invention;
FIG. 5 is a schematic view of the upper and lower transmission shafts in vertical engagement according to the present invention;
FIG. 6 is a schematic view showing a state where the wiper blade of the present invention is mounted;
FIG. 7 is an overall external structural view of the present invention;
in the figure, 1, incubator; 2. a box cover; 3. a first bearing; 4. a first rotating shaft; 5. an upper transmission disc; 6. an upper transmission shaft; 7. an upper connecting rod; 8. a hinge wheel; 9. a lower connecting rod; 10. a second bearing; 11. a second rotating shaft; 12. a lower transmission disc; 13. a lower transmission shaft; 14. cleaning the disc; 15. brushing; 16. a connector; 17. a connecting cylinder; 18. a fixing plate; 19. scraping a blade; 20. a water tank; 21. a motor; 22. a sloping block; 23. mounting a plate; 24. a spray head; 25. a drive motor; 26. a connecting rod; 27. a control panel; 28. a constant temperature control box; 29. a temperature sensor; 30. a cell inlet; 31. a culture solution inlet; 32. a liquid level window; 33. a water filling port; 34. a cell outlet; 35. a valve; 36. and (4) an observation window.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1-7, the present invention provides the following technical solutions: a cell culture device comprises an incubator 1, the upper end surface of the incubator 1 is detachably connected with a case cover 2, the upper end surface of the case cover 2 is provided with a motor 21, the output end of the motor 21 is fixedly connected with a first rotating shaft 4, the lower end surface of the case cover 2 is fixedly connected with a first bearing 3, the lower end surface of the first rotating shaft 4 sequentially penetrates through the case cover 2 and the first bearing 3 and extends to the lower part of the first bearing 3, the first rotating shaft 4 is rotatably connected with the first bearing 3, the lower end surface of the first rotating shaft 4 is fixedly connected with an upper transmission disc 5, the lower end surface of the upper transmission disc 5 is fixedly connected with a plurality of upper transmission shafts 6 distributed in a circular ring shape, the right side wall of the first bearing 3 is fixedly connected with an upper connecting rod 7, the lower end surface of the upper connecting rod 7 is fixedly connected with a hinge wheel 8, the outer wall of the hinge wheel 8 is movably connected with a, a second rotating shaft 11 is rotatably connected inside the second bearing 10, a lower transmission disc 12 is fixedly connected on the upper end surface of the second rotating shaft 11, a plurality of lower transmission shafts 13 distributed in a circular ring shape are fixedly connected on the upper end surface of the lower transmission disc 12, the plurality of lower transmission shafts 13 are meshed with the plurality of upper transmission shafts 6, a cleaning disc 14 is fixedly connected on the lower end surface of the second rotating shaft 11, bristles 15 are arranged on the lower end surface of the cleaning disc 14, connectors 16 are fixedly connected on the left side and the right side of the cleaning disc 14, connecting cylinders 17 are detachably connected on the opposite sides of the two connectors 16, fixed plates 18 are fixedly connected on the opposite sides of the two connecting cylinders 17, scraping blades 19 are fixedly connected on the opposite sides of the two fixed plates 18, the outer walls of the two scraping blades 19 are respectively abutted against the left side and the right side of the inner wall of the incubator 1, a water tank 20 is arranged on the right side wall of the incubator 1, oblique, the equal fixedly connected with mounting panel 23 of up end of two sloping blocks 22, a plurality of shower nozzles 24 are all installed to the up end of two mounting panels 23, and are a plurality of shower nozzle 24 all communicates with water tank 20 through the pipeline.
In this embodiment: after the incubator 1 is used, the motor 21 is started, the motor 21 drives the first rotating shaft 4 to rotate, the first bearing 3 enables the first rotating shaft 4 to rotate more stably, the first rotating shaft 4 drives the upper transmission disc 5 and the upper transmission shafts 6 at the lower end to rotate, the lower transmission disc 12 is meshed with the upper transmission shafts 6 through the lower transmission shafts 13, so that the lower transmission disc 12 is driven to rotate, the driving motor 25 is started, the driving motor 25 drives the lower connecting rod 9 to rotate ninety degrees anticlockwise, the lower connecting rod 9 drives the second bearing 10 to rotate ninety degrees anticlockwise, so that the lower transmission shaft 13 and the upper transmission shafts 6 rotate ninety degrees anticlockwise, the upper transmission shafts 6 are vertically meshed with the lower transmission shafts 13, the lower transmission shafts 13 can still be driven to rotate, and the cleaning disc 14 at the lower end of the second rotating shaft 11 is parallel to the right side wall of the incubator 1, the brush hair 15 is abutted against the side wall of the incubator 1, the motor 21 drives the cleaning disc 14 to rotate when working, thereby rotating and cleaning the side wall of the incubator 1, simultaneously starting the plurality of spray heads 24 at the bottom, the plurality of spray heads 24 spray water inside the water tank 20, thereby spraying and washing the inside of the incubator 1, the cleaning effect is greatly improved by matching with the brush hair 15, after the preliminary cleaning is finished, the driving motor 25 is started again to drive the lower connecting rod 9 to rotate ninety degrees clockwise, thereby enabling the lower driving disc 12 and the lower driving shaft 13 to return to the original positions, at the moment, the cleaning disc 14 is vertical to the side wall of the incubator 1, then the scraping blades 19 are sleeved on the connecting head 16 through the connecting cylinder 17 to complete the installation, after the installation, the two scraping blades 19 are respectively abutted against the two side walls of the incubator 1, the motor 21 drives the cleaning disc 14 to rotate, thereby driving the two scraping blades 19 to rotate, and scraping off suspended cells covered on the inner, further improving the cleaning effect, thereby keeping the interior of the incubator 1 clean and being beneficial to the next cell culture work.
As a technical optimization scheme of the invention, the upper transmission disc 5 and the lower transmission disc 12 are identical in structural size, and the upper connecting rod 7 and the lower connecting rod 9 are identical in structural size.
In this embodiment: go up drive disk 5 and lower drive disk 12's structure size homogeneous phase to can intermeshing play driven effect, go up connecting rod 7 and lower connecting rod 9 and pass through 8 swing joint of hinge, drive disk 12 is rotatory under the convenient drive.
As a technical optimization scheme of the invention, a driving motor 25 is installed on the front end face of the hinge wheel 8, and the output end of the driving motor 25 penetrates through the hinge wheel 8 and extends to the back of the hinge wheel 8 to be fixedly connected with the upper connecting rod 7.
In this embodiment: the driving motor 25 is convenient for driving the lower connecting rod 9 to rotate, and the control is convenient.
As a technical optimization scheme of the invention, two connecting rods 26 are fixedly connected to the right side wall of the incubator 1, and a control panel 27 is fixedly connected to the right side walls of the two connecting rods 26.
In this embodiment: the connecting rod 26 has the effect of fixing the control panel 27, and the control panel 27 is arranged to facilitate control of the operating equipment, so that the automation degree is high.
As a technical optimization scheme of the invention, a constant temperature control box 28 is arranged on the left side wall of the incubator 1, and the constant temperature control box 28 is electrically connected with the control panel 27.
In this embodiment: the temperature inside the incubator 1 is controlled by the thermostatic control chamber 28, thereby facilitating the adjustment of the temperature suitable for cell culture.
As a technical optimization scheme of the invention, a temperature sensor 29 is arranged at the right side of the top end in the incubator 1, and the temperature sensor 29 is electrically connected with the control panel 27.
In this embodiment: the temperature sensor 29 can detect the temperature inside the incubator 1 in real time and display the temperature through the display screen at the front end of the control panel 27, so that the temperature can be conveniently observed and adjusted.
As a technical optimization scheme of the invention, the left side of the upper end surface of the incubator 1 is provided with a cell inlet 30 and a culture solution inlet 31, and the cell inlet 30 is positioned on the left side of the culture solution inlet 31.
In this embodiment: the cell inlet 30 facilitates the insertion of cells into the incubator 1, and the culture solution inlet 31 facilitates the introduction of the cell culture solution.
As a technical optimization scheme of the invention, the outer wall of the water tank 20 is provided with a liquid level window 32, and the upper end surface of the water tank 20 is provided with a water filling port 33.
In this embodiment: the residual amount of water in the water tank 20 can be observed more visually through the liquid level window 32, so that water can be added through the water filling port 33 in time.
As a technical optimization scheme of the invention, the bottom end of the outer wall of the incubator 1 is provided with a cell outlet 34, and the outer wall of the cell outlet 34 is provided with a valve 35.
In this embodiment: after the cell culture is finished, the valve 35 is opened to discharge the cell culture solution through the cell outlet 34, so that the use is convenient.
As a technical optimization scheme of the invention, the outer wall of the incubator 1 is provided with an observation window 36.
In this embodiment: the user can observe the operation of the inside of the incubator 1 through the observation window 36.
The working principle and the using process of the invention are as follows: firstly, the cell inlet 30 is convenient for inserting cells into the incubator 1, the cell culture solution is placed into the incubator 1 through the culture solution inlet 31 for culture, after the incubator 1 is used, the motor 21 is started, the motor 21 drives the first rotating shaft 4 to rotate, the first bearing 3 enables the first rotating shaft 4 to rotate more stably, the first rotating shaft 4 drives the upper transmission disc 5 and the upper transmission shafts 6 at the lower end to rotate, the lower transmission disc 12 is meshed with the upper transmission shafts 6 through the lower transmission shafts 13, so the lower transmission disc 12 is driven to rotate, the driving motor 25 is started at the same time, the driving motor 25 drives the lower connection rod 9 to rotate ninety degrees counterclockwise, at the moment, the lower connection rod 9 drives the second bearing 10 to rotate ninety degrees counterclockwise, so that the lower transmission shaft 13 and the upper transmission shafts 6 rotate ninety degrees counterclockwise, at the moment, the upper transmission shafts 6 are vertically meshed with the lower transmission shafts 13, the lower transmission shaft 13 can still be driven to rotate, at the moment, the cleaning disc 14 at the lower end of the second rotating shaft 11 is parallel to the right side wall of the incubator 1, the bristles 15 are abutted to the side wall of the incubator 1, the motor 21 drives the cleaning disc 14 to rotate when working, so that the side wall of the incubator 1 is rotated and cleaned, a plurality of spray heads 24 at the bottom are simultaneously started, the plurality of spray heads 24 spray water inside the water tank 20, so that the inside of the incubator 1 is sprayed and washed, the cleaning effect is greatly improved by matching with the bristles 15, after the preliminary cleaning is finished, the driving motor 25 is started again to drive the lower connecting rod 9 to rotate ninety degrees clockwise, so that the lower transmission disc 12 and the lower transmission shaft 13 are both reset, at the moment, the cleaning disc 14 is vertical to the side wall of the incubator 1, then the scraping blades 19 are sleeved on the connecting head 16 through the connecting cylinder 17 to complete the installation, and after the installation, the, the starter motor 21 then drives the cleaning disc 14 to rotate, thereby driving the two scraping blades 19 to rotate, scraping off the suspended cells stained on the inner wall side of the incubator 1, further improving the cleaning effect, keeping the inside of the incubator 1 clean, and being beneficial to the next cell culture work.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The utility model provides a cell culture device, includes incubator (1), the up end of incubator (1) can be dismantled and is connected with case lid (2), its characterized in that: the upper end face of the box cover (2) is provided with a motor (21), the output end of the motor (21) is fixedly connected with a first rotating shaft (4), the lower end face of the box cover (2) is fixedly connected with a first bearing (3), the lower end face of the first rotating shaft (4) sequentially penetrates through the box cover (2) and the first bearing (3) and extends to the lower part of the first bearing (3), the first rotating shaft (4) is rotatably connected with the first bearing (3), the lower end face of the first rotating shaft (4) is fixedly connected with an upper transmission disc (5), the lower end face of the upper transmission disc (5) is fixedly connected with a plurality of upper transmission shafts (6) distributed in a circular ring shape, the right side wall of the first bearing (3) is fixedly connected with an upper connecting rod (7), the lower end face of the upper connecting rod (7) is fixedly connected with a hinge wheel (8), the outer wall of the hinge wheel (8) is movably connected with a lower connecting rod (9), the other end of the lower connecting rod (9) is fixedly connected with a second bearing (10), the inside of the second bearing (10) is connected with a second rotating shaft (11) in a rotating manner, the upper end face of the second rotating shaft (11) is fixedly connected with a lower transmission disc (12), the upper end face of the lower transmission disc (12) is fixedly connected with a plurality of lower transmission shafts (13) distributed in a circular ring shape, the lower transmission shafts (13) are meshed with the upper transmission shafts (6), the lower end face of the second rotating shaft (11) is fixedly connected with a cleaning disc (14), the lower end face of the cleaning disc (14) is provided with bristles (15), the left side and the right side of the cleaning disc (14) are both fixedly connected with connectors (16), one side of the two connectors (16) opposite to each other is detachably connected with a connecting cylinder (17), and one side of the two connecting cylinders (17) opposite to each other is both fixedly connected with a, two equal fixedly connected with doctor-bar (19) in one side that fixed plate (18) carried on the back mutually, two the outer wall of doctor-bar (19) respectively with the inner wall left and right sides butt of incubator (1), the right side wall of incubator (1) is provided with water tank (20), the equal fixedly connected with sloping block (22) in the inside bottom left and right sides of incubator (1), two the equal fixedly connected with mounting panel (23) of up end of sloping block (22), two a plurality of shower nozzles (24) are all installed to the up end of mounting panel (23), and are a plurality of shower nozzle (24) all communicate with water tank (20) through the pipeline.
2. A cell culture apparatus according to claim 1, wherein: the structure size of going up drive plate (5) and lower drive plate (12) is all the same, the structure size of going up connecting rod (7) and lower connecting rod (9) is all the same.
3. A cell culture apparatus according to claim 1, wherein: the front end face of the hinge wheel (8) is provided with a driving motor (25), and the output end of the driving motor (25) penetrates through the hinge wheel (8) and extends to the back of the hinge wheel (8) to be fixedly connected with the upper connecting rod (7).
4. A cell culture apparatus according to claim 1, wherein: the right side wall of the incubator (1) is fixedly connected with two connecting rods (26), and the right side wall of the two connecting rods (26) is fixedly connected with a control panel (27).
5. A cell culture apparatus according to claim 4, wherein: the left side wall of incubator (1) is provided with thermostatic control case (28), thermostatic control case (28) and control panel (27) electric connection.
6. A cell culture apparatus according to claim 4, wherein: the inside top right side of incubator (1) is provided with temperature sensor (29), temperature sensor (29) and control panel (27) electric connection.
7. A cell culture apparatus according to claim 1, wherein: the left side of the upper end face of the incubator (1) is provided with a cell inlet (30) and a culture solution inlet (31), and the cell inlet (30) is positioned on the left side of the culture solution inlet (31).
8. A cell culture apparatus according to claim 1, wherein: the outer wall of the water tank (20) is provided with a liquid level window (32), and the upper end face of the water tank (20) is provided with a water filling port (33).
9. A cell culture apparatus according to claim 1, wherein: the cell culture box is characterized in that a cell outlet (34) is formed in the bottom end of the outer wall of the culture box (1), and a valve (35) is arranged on the outer wall of the cell outlet (34).
10. A cell culture apparatus according to claim 1, wherein: the outer wall of incubator (1) is provided with observation window (36).
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| CN202110287717.9A CN113046239B (en) | 2021-03-17 | 2021-03-17 | Cell culture device |
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| CN202110287717.9A CN113046239B (en) | 2021-03-17 | 2021-03-17 | Cell culture device |
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| CN117903911A (en) * | 2024-01-22 | 2024-04-19 | 山东省核工业二四八地质大队 | Microorganism culture device for soil pollution treatment |
| CN118006461A (en) * | 2023-12-28 | 2024-05-10 | 武汉奇凯信息科技发展有限公司 | Cell separation culture device and method based on visual accurate identification |
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| CN113046239B (en) | 2024-06-28 |
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