CN113008367A - Laser light intensity three-dimensional distribution measuring system and measuring method - Google Patents
Laser light intensity three-dimensional distribution measuring system and measuring method Download PDFInfo
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Abstract
The invention discloses a laser light intensity three-dimensional distribution measuring system, which is characterized in that: the device comprises a YAG laser, a laser beam expanding device, a body laser induced fluorescence signal generating device, a power meter, a beam collector, a fluorescence signal detecting system, a data collecting and processing device and a signal generator; YAG laser sends out after laser beam expander and power meter, locate at the end of the laser light path by the beam collector, in order to stop the high-energy laser beam from continuing to spread; the fluorescence signal detection system is arranged at one side of the body laser induced fluorescence signal generation device, and the body laser induced fluorescence signal generation device comprises a cuvette containing rhodamine-ethanol solution, wherein the rhodamine can be excited by the expanded body laser beam to generate a body laser induced fluorescence signal; the fluorescence signal detection system transmits the collected body laser induced fluorescence signal to the data collection and processing device; the signal generator is respectively connected with the Nd-YAG laser and the fluorescence signal detection system through network cables, and the Nd-YAG laser and the fluorescence signal detection system are synchronized through the signal generator.
Description
The technical field is as follows:
the invention relates to the technical field of computational imaging, laser and spectrum, photoelectric detection and digital image processing, in particular to a system and a method for measuring three-dimensional distribution of laser light intensity.
Background art:
with the development and breakthrough of scientific technology, the laser technology plays an important role in national defense construction and national economy construction. Laser has been widely used in military, industrial, medical and other fields due to its good coherence, monochromaticity, high brightness, high energy density and other features.
The energy distribution of the laser is related to the quality of the laser beam, and has very important significance on the design, optimization and application of the laser. In practical applications, the laser energy distribution perpendicular to the propagation direction of the beam tends to be uneven due to the influence of the propagation medium and other factors. Therefore, accurate measurement of laser energy distribution is of great importance to the wide application of laser technology in a plurality of fields.
At present, the laser energy distribution measuring methods mainly include a scanning sampling method, an array camera detection method, a light spot imaging method and the like, but the methods mainly have the following defects:
1) the laser beam is required to be spatially sampled in the measuring process, and the original energy distribution of the laser beam is influenced more or less in the sampling process;
2) the detection methods can only realize the detection of the energy distribution on the two-dimensional cross section of the laser beam, but the actual laser beam has structures such as beam waist and the like, and the three-dimensional energy distribution of the laser beam cannot be detected in single measurement by the methods;
3) the detection equipment adopted by the methods is complex and the test precision is not high.
The non-invasive measurement method has become a mainstream technology in the field of modern flow field detection due to the characteristics of quick dynamic responsiveness and no interference on a field to be detected. Among them, optical diagnostic techniques involving the fields of laser and spectroscopy, photodetection, digital image processing, and the like are widely used.
In recent years, laser-induced fluorescence technology has been used to make visual measurements of key components in the flow field. Laser-induced fluorescence is essentially a photoluminescence phenomenon, in which when a specific component is irradiated with incident light of a specific wavelength, a dielectric photon absorbs the energy of the incident light and transits to an excited state, and a photon in the excited state transits to a ground state by spontaneous radiation, while emitting a fluorescence photon having a wavelength longer than that of the incident light and an intensity proportional to the concentration of the specific component.
Therefore, by adopting a non-invasive laser induced fluorescence technology, the specific components in the uniformly mixed solution are excited by laser to release fluorescence, and the visualization of the three-dimensional distribution of the laser intensity can be indirectly realized.
Meanwhile, by combining the tomography technology, the three-dimensional distribution of the laser intensity can be reconstructed. The method comprises the steps of simultaneously measuring a target field to be measured at a plurality of angles by adopting a plurality of imaging devices, and realizing three-dimensional measurement of the target field to be measured by combining a chromatographic reconstruction algorithm.
However, there are also two problems:
1) the solution may absorb the laser energy to a certain extent, which affects the measurement of the laser energy;
2) the use of multiple imaging devices requires extremely high experimental costs.
The invention content is as follows:
aiming at the defects in the prior art, the invention provides a laser intensity three-dimensional distribution measuring system and a measuring method, which adopt a non-invasive measuring method, namely a bulk laser induced fluorescence technology, and measure the absorptivity of a medium solution based on the beer-lambert law;
meanwhile, by means of the endoscopic tomography technology, the problems of high manufacturing cost of the traditional tomography technology are solved while the advantages of the traditional tomography technology are kept. The absorption effect of the solution is considered in the reconstruction process of the laser light intensity three-dimensional distribution, and an alternate iterative reconstruction algorithm is adopted to realize the reconstruction of the laser light intensity three-dimensional distribution after absorption and correction and indirectly realize the measurement of the laser light intensity three-dimensional distribution.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a laser light intensity three-dimensional distribution measurement system is characterized in that: the device comprises a YAG laser, a laser beam expanding device, a body laser induced fluorescence signal generating device, a power meter, a beam collector, a fluorescence signal detecting system, a data collecting and processing device and a signal generator; YAG laser sends out after laser beam expander and power meter, locate at the end of the laser light path by the beam collector, in order to stop the high-energy laser beam from continuing to spread; the fluorescence signal detection system is arranged at one side of the body laser induced fluorescence signal generation device, and the body laser induced fluorescence signal generation device comprises a cuvette containing rhodamine-ethanol solution, wherein the rhodamine can be excited by the expanded body laser beam to generate a body laser induced fluorescence signal; the fluorescence signal detection system transmits the collected body laser induced fluorescence signal to the data collection and processing device; the signal generator is respectively connected with the Nd-YAG laser and the fluorescence signal detection system through network cables, and the Nd-YAG laser and the fluorescence signal detection system are synchronized through the signal generator.
In one embodiment, the laser beam expanding device comprises a plano-concave spherical mirror and a plano-convex spherical mirror.
In one embodiment, the fluorescence signal detection system comprises a plurality of first lenses, a multi-lens endoscope, a notch filter, a second lens and a high-speed camera;
the multi-split endoscope comprises a plurality of incident ends and an emergent end, wherein the front end of the incident end is provided with a first lens, and the first lenses are uniformly distributed on the circumference of the same radius outside the bulk laser induced fluorescence signal generating device;
a second lens is arranged at the front end of the high-speed camera, a notch filter is arranged at the front end of the second lens, and the notch filter and the emergent end are arranged at intervals;
the fluorescence signal detection system is in communication connection with the data acquisition and processing device through a network cable.
A measuring method of a laser light intensity three-dimensional distribution measuring system is characterized in that: comprises the following steps of (a) carrying out,
s1: establishing a set of body laser induced fluorescence signal detection system based on endoscopic chromatography;
s2: measuring the absorptivity of rhodamine-ethanol solution in a laser induced fluorescence signal generation device;
s3: the YAG laser and the fluorescence signal detection system are synchronized through a signal generator;
s4: the fluorescence signal detection system transmits the collected multi-angle body laser induced fluorescence signals to the data collection and processing device;
s5: the data acquisition and processing device carries out data processing on the multi-angle laser induced fluorescence signals to obtain the three-dimensional distribution of the laser light intensity after absorption and correction.
In one embodiment, the step of S5 further includes the step of,
s51: when the body laser induced fluorescence signal generating device is not placed and the Nd-YAG laser device is not in work, a calibration plate with a black-white grid is placed at the same position as the body laser induced fluorescence signal generating device, a fluorescence signal detection system is adopted to collect a calibration plate picture, and required calibration parameters are obtained through a calibration algorithm;
s52: taking down the calibration plate and placing the body laser induced fluorescence signal generating device to enable the Nd, namely the YAG laser to work at the frequency doubling frequency, namely the light emitting wavelength is 532nm, and adopting a signal generator to synchronize the Nd, the YAG laser and the fluorescence signal detecting system to record the body laser induced fluorescence signal and send the body laser induced fluorescence signal to the data collecting and processing device for storage;
s53: establishing a fluorescence signal field inversion model by combining the beer-Lambert law and adopting a Monte Carlo ray tracing method;
s54: and solving the inversion problem by combining the inversion model of the fluorescence signal field and adopting a chromatography alternate iterative reconstruction algorithm, and reconstructing the bulk laser induced fluorescence field so as to obtain the three-dimensional distribution of the light intensity of the laser beam to be detected.
In one embodiment, the step of S51 further includes the step of,
s511: the fluorescence signal detection system collects black and white lattice images on the multi-angle calibration plate through a one-to-many endoscope;
s512: extracting black and white grid angular points in the multi-angle calibration plate photo to obtain coordinates of the multi-angle calibration plate photo in a world coordinate system and a camera coordinate system;
s513: and obtaining required calibration parameters by combining coordinates of the black and white lattice corner points under a world coordinate system and a camera coordinate system through a Zhang Zhengyou calibration algorithm, and determining the position relationship among the detection angles of the fluorescence signal detection system and the position relationship between the fluorescence signal detection system and the laser-induced fluorescence signal field to be detected according to the calibration parameters.
In one embodiment, the Nd-YAG laser works at double frequency, and the laser beam expanding device consists of a plano-concave spherical mirror and a plano-convex spherical mirror, and the focal length of the laser beam expanding device is determined according to the diameter of an emergent light spot of the laser and the required beam expanding multiple.
In one embodiment, the bulk laser induced fluorescence signal generating device comprises a cuvette containing ethanol diluted rhodamine solution, wherein rhodamine can be excited by expanded 532nm bulk laser beams to generate bulk laser induced fluorescence signals; the cuvette is of a capless design and made of a piece of JGS-1 quartz glass, the end face dimension of the cuvette being larger than the diameter of the expanded bulk laser beam.
In one embodiment, the notch filter has a center wavelength of 532nm, a bandwidth of 20nm and an OD of 6, so that the detected laser-induced fluorescence signal is free from interference of the laser signal.
In one embodiment, the rhodamine-ethanol solution absorbs the laser signal to produce a laser induced fluorescence signal.
In one embodiment, the absorbance of the rhodamine-ethanol solution can be calculated by measuring the laser energy before and after the laser beam passes through the cuvette using a power meter and using beer-lambert law.
The invention has the main beneficial effects that: the invention adopts a non-invasive optical diagnosis method, namely a body laser induced fluorescence technology, combines an endoscopic chromatography technology, calculates the absorptivity of a medium solution through the beer-Lambert law, and adopts an alternate iterative reconstruction algorithm to reconstruct a body laser induced fluorescence signal field which is subjected to absorption correction, thereby indirectly realizing the three-dimensional distribution measurement of laser intensity. The method can well solve the problems existing in the prior laser light intensity distribution measurement, and realize high-precision laser light intensity three-dimensional distribution measurement while reducing the experiment cost.
Description of the drawings:
the above and other features, properties and advantages of the present invention will become more apparent from the following description of the embodiments with reference to the accompanying drawings in which like reference numerals denote like features throughout the several views, wherein:
FIG. 1 is a schematic structural diagram of a bicolor radiation temperature measurement system based on endoscopic tomography in a laser intensity three-dimensional distribution measurement system according to an embodiment of the present invention;
fig. 2 is a schematic top view of a projection process of light rays emitted from a fluorescence signal field to be measured on a pixel (s, t) in a measurement system for three-dimensional distribution of laser intensity according to an embodiment of the present invention.
In the illustration:
YAG 1-Nd laser
2-plano-concave spherical mirror
3-plano-convex spherical mirror
4-body laser induced fluorescence signal generating device
5-power meter
6-beam current collector
7-first lens
8-incident end
9-endoscope
10-exit end
11-notch filter
12-second lens
13-high speed camera
14-data acquisition and processing device
15-signal generator.
The specific implementation mode is as follows:
the present invention will be described in detail with reference to specific examples. The following examples will help those skilled in the art to further understand the present invention, but are not limited thereto, and all modifications or equivalent substitutions can be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention. It should be noted that the relationships depicted in the figures are for illustrative purposes only and are not to be construed as limiting the patent.
Referring to fig. 1 in conjunction with fig. 2, a method for measuring three-dimensional distribution of laser intensity is as follows,
s1: establishing a set of body laser induced fluorescence signal detection system based on endoscopic chromatography; the endoscopic chromatography-based body laser-induced fluorescence signal detection system comprises: a Nd, a YAG laser 1, a laser beam expanding device, an integrated laser induced fluorescence signal generating device 4, a power meter 5, a beam collector 6, a fluorescence signal detecting system and a data collecting and processing device 14;
the laser beam expanding device comprises a plano-concave spherical mirror 2 and a plano-convex spherical mirror 3; the body laser induced fluorescence signal generating device 4 comprises a cuvette containing rhodamine-ethanol solution;
the fluorescence signal detection system comprises a plurality of first lenses 7, a one-to-many endoscope 9, a notch filter 11, a second lens 12 and a high-speed camera 13;
the one-to-many endoscope 9 comprises a plurality of incident ends 8 and an emergent end 10, and the incident ends 8 and the first lens 7 are uniformly arranged on the circumference of the same radius outside the bulk laser induced fluorescence signal generating device 4; the fluorescence signal detection system is in communication connection with the data acquisition and processing device 14 through a network cable;
s2: measuring the absorption rate of rhodamine-ethanol solution in the body laser induced fluorescence signal generation device 4;
s3: the Nd is that the YAG laser 1 and the fluorescence signal detection system are synchronized through a signal generator 15;
s4: the fluorescence signal detection system transmits the collected multi-angle body laser induced fluorescence signals to the data collecting and processing device 14;
s5: the data acquisition and processing device 14 performs data processing on the multi-angle laser-induced fluorescence signal to obtain three-dimensional distribution of laser intensity after absorption correction.
The Nd-YAG laser works at double frequency, and the light-emitting wavelength of the Nd-YAG laser is 532 nm.
The laser beam expanding device is composed of a plano-concave spherical mirror 2 and a plano-convex spherical mirror 3, and the focal length of the laser beam expanding device is determined according to the diameter of a light-emitting spot of the laser and the required beam expanding multiple.
The body laser induced fluorescence signal generating device is a cuvette containing ethanol diluted rhodamine solution, wherein rhodamine can be excited by expanded 532nm body laser beams to generate body laser induced fluorescence signals.
The cuvette can be a uncovered cuvette made of JGS-1 quartz glass, and the size of the end face of the cuvette is larger than the diameter of the expanded bulk laser beam.
The beam dump 6 may be placed at the end of the laser path to block the continued propagation of the high energy laser beam.
The notch filter 11 has a central wavelength of 532nm, a bandwidth of 20nm and an OD of 6, and has an effect of preventing interference of a laser signal in a detected laser-induced fluorescence signal.
The rhodamine-ethanol solution absorbs the laser signal to generate a laser induced fluorescence signal.
The absorptivity of the rhodamine-ethanol solution can be obtained by measuring the laser energy before and after a laser beam passes through the cuvette by a power meter and calculating by adopting the beer-lambert law.
The step of S5 further includes the steps of:
s51: when the body laser induced fluorescence signal generating device 4 and the Nd-YAG laser 1 are not placed yet, a calibration plate with a black-white grid is placed at the same position, a fluorescence signal detection system is adopted to collect a calibration plate picture, and required calibration parameters are obtained through a calibration algorithm;
s52: taking down the calibration plate and placing the body laser induced fluorescence signal generating device 4 to enable the Nd, namely the YAG laser to work at the frequency doubling frequency, namely the light-emitting wavelength is 532nm, and adopting a signal generator to synchronize the Nd, the YAG laser and a fluorescence signal detection system, recording the body laser induced fluorescence signal and sending the body laser induced fluorescence signal to the data acquisition and processing device for storage;
s53: establishing a fluorescence signal field inversion model by combining the beer-Lambert law and adopting a Monte Carlo ray tracing method;
s54: and solving the inversion problem by combining the inversion model of the fluorescence signal field and adopting an alternating iterative reconstruction algorithm, and reconstructing the bulk laser induced fluorescence field so as to obtain the three-dimensional distribution of the light intensity of the laser beam to be detected.
The calibration process in the step S51 further includes the steps of:
s511: the fluorescence signal detection system collects black and white lattice images on the multi-angle calibration plate through a one-to-many endoscope;
s512: extracting black and white grid angular points in the multi-angle calibration plate photo to obtain coordinates of the multi-angle calibration plate photo in a world coordinate system and a camera coordinate system;
s513: and obtaining required calibration parameters by combining coordinates of black and white lattice corner points in a world coordinate system and a camera coordinate system through a Zhang-Yongyou calibration algorithm, and determining the position relationship among the detection angles of the fluorescence signal detection system and the position relationship between the fluorescence signal detection system and the laser-induced fluorescence signal field to be detected according to the calibration parameters.
And S53, establishing a fluorescence signal field inversion model, wherein the process is as follows:
referring to fig. 2, combining the monte carlo photon tracking method, it can be known that the mathematical expression of the light intensity of a certain pixel point on the camera chip can be expressed as:
p(s，t)＝∫∫∫I(xw，yw，zw)·W(xi，yi，zi，s，t)dV, (1)
wherein p (s, t) is the intensity measured at the pixel center point (s, t); i (x)w，yw，zw) Is a certain point (x) in the world coordinate systemw，yw，zw) The laser induced fluorescence intensity of (a); w represents a weight matrix, the physical meaning of which is the intensity distribution of a point source of light with unit intensity imaged on the camera chip, i.e. the point spread function. (s, t) and (x)w，yw，zw) The cell indices on the camera chip coordinate system and in the world coordinate system, respectively. If the laser-induced fluorescence field to be detected is discretized, N is formed in the directions of three coordinate axes of x, y and zx、Ny、NzPer voxel, the above equation can be further expressed as:
wherein, N represents the discretized total prime number; Δ x, Δ y, Δ z are the dimensions of the voxels along the three coordinate axes, respectively. Therefore, each pixel on the camera provides a linear equation system, and the variable in the equation is the intensity of the bulk laser induced fluorescence signal of all voxels in the reconstruction region. However, when there is an absorption effect of the rhodamine-ethanol solution in the fluorescence signal generation device 4, the above fluorescence signal field inversion model should be expressed as follows, in combination with beer-lamber's law:
wherein α is an absorption rate of the uniformly mixed rhodamine-ethanol solution, measured in the step S2, liIs the propagation distance of the laser induced fluorescence signal in the ith voxel.
When laser-induced fluorescence signals in regions to be detected at different angles are recorded simultaneously, a plurality of sets of linear equations can be obtained, which are expressed in a vector form as follows:
the mathematical expression for solving the principle of the bulk laser induced fluorescence signal field by using an alternating iterative reconstruction algorithm described in the step S54 is as follows:
wherein the content of the first and second substances,
representing the determined voxels (x) in an iterative processi，yi，zi) The superscript k represents the kth iteration. Lambda [ alpha ]ARTFor the relaxation factor, the convergence rate and convergence of the iteration can be controlled, and in this embodiment, the values are takenIs 0.7. Ws，tIs a weight coefficient corresponding to the pixel (s, t).
Thus, the difference between the true projection values and the reconstructed values is taken into account during each iteration. Finally, by setting an iteration termination condition: the iteration residual is smaller than the set value or the iteration times are larger than the set value, and the iteration is terminated, in this embodiment, the iteration residual is less than 10-6The maximum number of iterations is 200.
It should be noted that the prior art in the protection scope of the present invention is not limited to the examples given in the present application, and all the prior art which is not inconsistent with the technical scheme of the present invention, including but not limited to the prior patent documents, the prior publications and the like, can be included in the protection scope of the present invention. In addition, the combination of the features in the present application is not limited to the combination described in the claims of the present application or the combination described in the embodiments, and all the features described in the present application may be freely combined or combined in any manner unless contradictory to each other. It should also be noted that the above-mentioned embodiments are only specific embodiments of the present invention. It is apparent that the present invention is not limited to the above embodiments and similar changes or modifications can be easily made by those skilled in the art from the disclosure of the present invention and shall fall within the scope of the present invention.
Claims (11)
1. A laser light intensity three-dimensional distribution measurement system is characterized in that: the device comprises a YAG laser, a laser beam expanding device, a body laser induced fluorescence signal generating device, a power meter, a beam collector, a fluorescence signal detecting system, a data collecting and processing device and a signal generator; wherein the content of the first and second substances,
after laser emitted by a YAG laser passes through a laser beam expanding device and a power meter, a beam collector is arranged at the tail end of a laser light path to block the continuous propagation of high-energy laser beams;
the fluorescence signal detection system is arranged at one side of the body laser induced fluorescence signal generation device, and the body laser induced fluorescence signal generation device comprises a cuvette containing rhodamine-ethanol solution, wherein the rhodamine can be excited by the expanded body laser beam to generate a body laser induced fluorescence signal;
the fluorescence signal detection system transmits the collected body laser induced fluorescence signal to the data collection and processing device;
the signal generator is respectively connected with the Nd-YAG laser and the fluorescence signal detection system through network cables, and the Nd-YAG laser and the fluorescence signal detection system are synchronized through the signal generator.
2. The laser light intensity three-dimensional distribution measuring system according to claim 1, characterized in that: the laser beam expanding device comprises a plano-concave spherical mirror and a plano-convex spherical mirror.
3. The laser light intensity three-dimensional distribution measuring system according to claim 2, characterized in that: the fluorescence signal detection system comprises a plurality of first lenses, a one-to-many endoscope, a notch filter, a second lens and a high-speed camera;
the multi-split endoscope comprises a plurality of incident ends and an emergent end, wherein the front end of the incident end is provided with a first lens, and the first lenses are uniformly distributed on the circumference of the same radius outside the bulk laser induced fluorescence signal generating device;
a second lens is arranged at the front end of the high-speed camera, a notch filter is arranged at the front end of the second lens, and the notch filter and the emergent end are arranged at intervals;
the fluorescence signal detection system is in communication connection with the data acquisition and processing device through a network cable.
4. A measuring method of the laser light intensity three-dimensional distribution measuring system as claimed in claim 3, characterized in that: comprises the following steps of (a) carrying out,
s1: establishing a set of body laser induced fluorescence signal detection system based on endoscopic chromatography;
s2: measuring the absorptivity of rhodamine-ethanol solution in a laser induced fluorescence signal generation device;
s3: the YAG laser and the fluorescence signal detection system are synchronized through a signal generator;
s4: the fluorescence signal detection system transmits the collected multi-angle body laser induced fluorescence signals to the data collection and processing device;
s5: the data acquisition and processing device carries out data processing on the multi-angle laser induced fluorescence signals to obtain the three-dimensional distribution of the laser light intensity after absorption and correction.
5. The measurement method according to claim 4, characterized in that: the step of S5 further includes the steps of,
s51: when the body laser induced fluorescence signal generating device is not placed and the Nd-YAG laser device is not in work, a calibration plate with a black-white grid is placed at the same position as the body laser induced fluorescence signal generating device, a fluorescence signal detection system is adopted to collect a calibration plate picture, and required calibration parameters are obtained through a calibration algorithm;
s52: taking down the calibration plate and placing the body laser induced fluorescence signal generating device to enable the Nd, namely the YAG laser to work at the frequency doubling frequency, namely the light emitting wavelength is 532nm, and adopting a signal generator to synchronize the Nd, the YAG laser and the fluorescence signal detecting system to record the body laser induced fluorescence signal and send the body laser induced fluorescence signal to the data collecting and processing device for storage;
s53: establishing a fluorescence signal field inversion model by combining the beer-Lambert law and adopting a Monte Carlo ray tracing method;
s54: and solving the inversion problem by combining the inversion model of the fluorescence signal field and adopting a chromatography alternate iterative reconstruction algorithm, and reconstructing the bulk laser induced fluorescence field so as to obtain the three-dimensional distribution of the light intensity of the laser beam to be detected.
6. The apparatus for detecting the three-dimensional distribution of laser intensity and the method for measuring the same as claimed in claim 5, wherein the step of S51 further comprises the steps of,
s511: the fluorescence signal detection system collects black and white lattice images on the multi-angle calibration plate through a one-to-many endoscope;
s512: extracting black and white grid angular points in the multi-angle calibration plate photo to obtain coordinates of the multi-angle calibration plate photo in a world coordinate system and a camera coordinate system;
s513: and obtaining required calibration parameters by combining coordinates of the black and white lattice corner points under a world coordinate system and a camera coordinate system through a Zhang Zhengyou calibration algorithm, and determining the position relationship among the detection angles of the fluorescence signal detection system and the position relationship between the fluorescence signal detection system and the laser-induced fluorescence signal field to be detected according to the calibration parameters.
7. The measurement method as claimed in claim 4, wherein the Nd: YAG laser operates at double frequency, the laser beam expanding device is composed of a plano-concave spherical mirror and a plano-convex spherical mirror, and the focal length is determined according to the diameter of the light spot of the laser and the required beam expanding multiple.
8. The measurement method according to claim 4, wherein the bulk laser induced fluorescence signal generation device comprises a cuvette containing diluted rhodamine solution in ethanol, wherein rhodamine can be excited by the expanded 532nm bulk laser beam to generate a bulk laser induced fluorescence signal; the cuvette is of a capless design and made of a piece of JGS-1 quartz glass, the end face dimension of the cuvette being larger than the diameter of the expanded bulk laser beam.
9. The method of claim 4, wherein the notch filter has a center wavelength of 532nm, a bandwidth of 20nm, and an OD of 6, such that the detected laser-induced fluorescence signal is free from interference of the laser signal.
10. The method of claim 4, wherein the rhodamine-ethanol solution absorbs the laser signal to produce a laser induced fluorescence signal.
11. The method according to claim 4, wherein the absorbance of the rhodamine-ethanol solution is calculated by measuring the laser energy of the laser beam before and after passing through the cuvette by a power meter and using the beer-Raney law.
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