CN112980663B - A collection and protection device for microorganism nucleic acid sample - Google Patents
A collection and protection device for microorganism nucleic acid sample Download PDFInfo
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- CN112980663B CN112980663B CN202110531379.9A CN202110531379A CN112980663B CN 112980663 B CN112980663 B CN 112980663B CN 202110531379 A CN202110531379 A CN 202110531379A CN 112980663 B CN112980663 B CN 112980663B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/22—Means for packing or storing viable microorganisms
Abstract
The invention discloses a collection and protection device for a microbial nucleic acid sample, which belongs to the technical field of microbial scientific research instruments and comprises a sampling head and a protective shell, wherein a supporting seat is fixedly arranged at the bottom of the protective shell, an arc-shaped rod is fixedly connected to the inner wall of the protective shell, an arc-shaped block in sliding connection with the arc-shaped rod is sleeved on the arc-shaped rod, first springs are fixedly connected between two sides of the arc-shaped block and the inner wall of the protective shell, the two first springs are sleeved outside the arc-shaped rod, a driving mechanism is arranged between the arc-shaped block and the inner bottom of the protective shell, a supporting block is fixedly connected to the upper end of the arc-shaped block, and a mixing tube is arranged above the supporting block. The invention can not only provide a clean and sanitary environment for the sample, so that the sample is not easily influenced by the external environment, but also protect the mixing tube and the liquid injection tube, so that the sample is not easily damaged, and the sample and the microbial nucleic acid protective liquid can be fully mixed and uniformly mixed.
Description
Technical Field
The invention relates to the technical field of microorganism scientific research instruments, in particular to a device for collecting and protecting a microorganism nucleic acid sample.
Background
Microorganisms are ubiquitous, large in quantity and diverse, and are closely related to human life and the surrounding environment. Microorganisms have many uses, and are currently applied to various fields such as industry, agriculture, medicine, and food, and thus it is very important to continuously and intensively study the composition, kind, and function of microorganisms in the human surroundings. At present, collection and transportation of microbial nucleic acid samples are one of important links in the research of microorganisms in clinical and scientific research.
During the process of collection and transportation of the microbial nucleic acid sample, if a proper sample collection device and transportation conditions are lacked, the workload of workers is increased, and the microbial nucleic acid is degraded, so that the follow-up research fails.
In the prior art, a device for collecting a microbial metabolite sample clinically cannot be effectively protected in the transportation process, and is inconvenient to operate due to the fact that part of operations are distributed; therefore, we have designed a collection and protection device for microbial nucleic acid samples.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a device for collecting and protecting a microbial nucleic acid sample.
In order to achieve the purpose, the invention adopts the following technical scheme:
a collection and protection device for a microbial nucleic acid sample comprises a sampling head and a protective shell, wherein a supporting seat is fixedly installed at the bottom of the protective shell, an arc rod is fixedly connected to the inner wall of the protective shell, an arc block which is in sliding connection with the arc rod is sleeved on the arc rod, first springs are fixedly connected between two sides of the arc block and the inner wall of the protective shell, the two first springs are sleeved outside the arc rod, a driving mechanism is arranged between the arc block and the inner bottom of the protective shell, a supporting block is fixedly connected to the upper end of the arc block, a mixing tube is arranged above the supporting block, an injection tube is installed at the bottom of the mixing tube, a plug is installed at the bottom of the injection tube, a limiting mechanism for limiting the mixing tube and the injection tube is arranged at the upper end of the supporting block, two arc plates are arranged inside the protective shell and are abutted against the outer wall of the mixing tube, and two all be equipped with between the one side that the arc carried on the back mutually and the inner wall of protective housing and support tight mechanism, the sampling head is located the hybrid tube, the inside of hybrid tube is equipped with annotates the liquid pipe, annotate two bracing pieces of outer wall fixedly connected with of liquid pipe, two the bracing piece offsets with the upper end of hybrid tube, the bottom of annotating the liquid pipe is installed and is not broken away from, the sampling head is installed in the bottom of bottom, the upper end of protective housing is equipped with the closing cap, be equipped with fixed establishment between closing cap and the protective housing, the bottom of closing cap is equipped with the arc wall, be equipped with pushing mechanism between arc wall and the notes liquid pipe.
Preferably, actuating mechanism is including installing the motor of bottom in the protective housing, the output shaft of motor is fixed with the cam, the cam offsets with the outer wall of arc piece, power and control button are installed to the outer wall of protective housing, motor, power and control button electric property series.
Preferably, the limiting mechanism comprises a first groove arranged on the supporting block, a second groove is arranged at the bottom of the first groove, and the mixing tube and the injection tube are respectively abutted against the first groove and the second groove.
Preferably, support tight mechanism include with the protective housing inner wall rotate be connected with the turning block, the inside of turning block is equipped with the inner chamber, inner chamber sliding connection has the sliding block, be fixed with the second spring between the inner wall of sliding block and inner chamber, the other end fixedly connected with dwang of sliding block, the dwang runs through the one end lateral wall of turning block and rather than sliding connection, the dwang is connected with the outer wall rotation of arc.
Preferably, the fixing mechanism comprises a first magnetic block fixed at the upper end of the protective shell, a second magnetic block is fixedly connected to the bottom of the sealing cover, the first magnetic block and the second magnetic block are abutted, and the opposite surfaces of the first magnetic block and the second magnetic block have opposite magnetic poles.
Preferably, pushing mechanism is including fixing the third spring at the mixed inside pipe wall, the outer wall cover of annotating the liquid pipe is equipped with the lantern ring, two thin poles of upper end fixedly connected with of lantern ring, annotate the liquid pipe and two thin poles all run through the third spring and rather than offset, two the common fixedly connected with cambered surface piece in upper end of thin pole, the cambered surface piece offsets with the inner wall of arc wall, the upper end fixed mounting who annotates the liquid pipe has the annular piece.
Preferably, the third spring is in a circular truncated cone shape, and the diameter of the upper end of the third spring is smaller than that of the lower end of the third spring.
Preferably, the liquid injection pipe is arranged in an inverted frustum shape.
Compared with the prior art, the invention has the beneficial effects that:
1. the motor work drives the cam and rotates, and the cam rotates the removal of drive arc piece, under the effect of first spring, can realize that the arc piece resets to realize that arc piece, supporting shoe, hybrid tube use the turning block as axle center reciprocating motion, thereby can realize sample and microorganism nucleic acid protection liquid intensive mixing and misce bene.
2. The mixing pipe and the liquid injection pipe can be protected by the protective shell, so that the mixing pipe and the liquid injection pipe are not easy to damage in carrying and transportation,
3. through closing cap and protective housing can make sample and microorganism nucleic acid protection liquid when mixing under inclosed environment, be difficult for receiving external environment's influence, more be favorable to the protection to the sample.
In conclusion, the invention can not only provide a clean and sanitary environment for the sample, so that the sample is not easily influenced by the external environment, but also protect the mixing tube and the liquid injection tube, so that the sample is not easily damaged, and the sample and the microbial nucleic acid protective solution can be fully mixed and uniformly mixed.
Drawings
FIG. 1 is a schematic structural diagram of a collection and protection device for a microbial nucleic acid sample according to the present invention;
FIG. 2 is an enlarged view of the structure A in FIG. 1;
FIG. 3 is a schematic diagram of a pressing mechanism of the apparatus for collecting and protecting a nucleic acid sample from a microorganism according to the present invention;
FIG. 4 is a schematic structural diagram of a limiting mechanism in the collection and protection device for a microbial nucleic acid sample according to the present invention;
FIG. 5 is a top view of an arc-shaped plate of the collection and protection device for a microbial nucleic acid sample according to the present invention;
FIG. 6 is a schematic structural diagram of a bottom cover of a device for collecting and protecting a microbial nucleic acid sample according to the present invention;
FIG. 7 is a cross-sectional view of a collar of the device for collecting and protecting a microbial nucleic acid sample according to the present invention.
In the figure: the device comprises a support seat 1, a protective shell 2, an arc-shaped rod 3, a first spring 4, an arc-shaped block 5, a motor 6, a cam 7, a power supply 8, a control button 9, a support rod 10, a support block 11, a mixing tube 12, a sampling head 13, a lantern ring 14, a thin rod 15, a liquid injection tube 16, an arc-shaped plate 17, a sealing cover 18, a second magnetic block 19, a first magnetic block 20, a first groove 21, an injection tube 22, a plug 23, a second groove 24, a rotating block 25, an inner cavity 26, a second spring 27, a sliding block 28, a rotating rod 29, an arc-shaped groove 30, an arc-shaped surface block 31, an annular block 32, a third spring 33 and a bottom cover 34.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-7, a collection and protection device for microorganism nucleic acid sample, including sampling head 13 and protective housing 2, the bottom fixed mounting of protective housing 2 has supporting seat 1, the inner wall fixedly connected with arc pole 3 of protective housing 2, the cover is equipped with rather than sliding connection's arc piece 5 on the arc pole 3, equal first spring 4 of fixedly connected with between the both sides of arc piece 5 and the inner wall of protective housing 2, two first spring 4 all overlap in the outside of arc pole 3, can realize the reseing of arc piece 5 through first spring 4, then the centre of a circle of arc pole 3 and the coincidence of the centre of a circle of turning block 25.
Be equipped with actuating mechanism between the interior bottom of arc piece 5 and protective housing 2, actuating mechanism is including installing motor 6 of bottom in protective housing 2, and the output shaft of motor 6 is fixed with cam 7, and cam 7 offsets with the outer wall of arc piece 5, and power 8 and control button 9 are installed to the outer wall of protective housing 2, and motor 6, power 8 and control button 9 electrical property are established ties, and motor 6 drives cam 7 and rotates and can rock or shake arc piece 5, can mix the liquid mixture to the microorganism.
The upper end fixedly connected with supporting shoe 11 of arc piece 5, the top of supporting shoe 11 is equipped with hybrid tube 12, injection tube 22 is installed to the bottom of hybrid tube 12, end cap 23 is installed to the bottom of injection tube 22, the upper end of supporting shoe 11 is equipped with the stop gear of restriction hybrid tube 12 and injection tube 22, stop gear is including setting up first recess 21 on supporting shoe 11, the bottom of first recess 21 is equipped with second recess 24, hybrid tube 12 and injection tube 22 offset with first recess 21 and second recess 24 respectively.
The inside of protective housing 2 is equipped with two arcs 17, two arcs 17 all offset with the outer wall of hybrid tube 12, and all be equipped with between the one side that two arcs 17 carried on the back mutually and the inner wall of protective housing 2 and support tight mechanism, support tight mechanism and include the turning block 25 who is connected with the rotation of protective housing 2 inner wall, the inside of turning block 25 is equipped with inner chamber 26, sliding connection has sliding block 28 in the inner chamber 26, be fixed with second spring 27 between the inner wall of sliding block 28 and inner chamber 26, sliding block 28's other end fixedly connected with dwang 29, dwang 29 runs through the one end lateral wall of turning block 25 and rather than sliding connection, dwang 29 is connected with the outer wall rotation of arcs 17, can carry out the centre gripping to hybrid tube 12.
The sampling head 13 is positioned in the mixing pipe 12, the liquid injection pipe 16 is arranged in the mixing pipe 12, the outer wall of the liquid injection pipe 16 is fixedly connected with two support rods 10, the two support rods 10 are abutted against the upper end of the mixing pipe 12, the bottom of the liquid injection pipe 16 is provided with a non-separated bottom cover 34, and the bottom cover 34 is connected with the liquid injection pipe 16 through an integrated rubber wire; the sampling head 13 is arranged at the bottom of the bottom cover 34, and the upper end of the protective shell 2 is provided with a sealing cover 18.
A fixing mechanism is arranged between the sealing cover 18 and the protective shell 2 and comprises a first magnetic block 20 fixed at the upper end of the protective shell 2, a second magnetic block 19 is fixedly connected to the bottom of the sealing cover 18, the first magnetic block 20 and the second magnetic block 19 are abutted, and opposite magnetic poles of the first magnetic block 20 and the second magnetic block 19 are opposite.
The bottom of the sealing cover 18 is provided with an arc-shaped groove 30, a pushing mechanism is arranged between the arc-shaped groove 30 and the liquid injection pipe 16, the pushing mechanism comprises a third spring 33 fixed on the inner wall of the mixing pipe 12, the outer wall of the liquid injection pipe 16 is sleeved with a lantern ring 14, the upper end of the lantern ring 14 is fixedly connected with two thin rods 15, the liquid injection pipe 16 and the two thin rods 15 both penetrate through the third spring 33 and abut against the third spring 33, the upper ends of the two thin rods 15 are fixedly connected with an arc surface block 31 together, the arc surface block 31 abuts against the inner wall of the arc-shaped groove 30, and the upper end of the liquid injection pipe 16 is fixedly provided with an annular block 32; the third spring 33 is in a circular truncated cone shape, and the diameter of the upper end of the third spring 33 is smaller than that of the lower end; the liquid injection pipe 16 is arranged in an inverted frustum shape; wherein the inner bottom of the collar 14 is arranged as a ring-shaped concave surface.
When the sampling device is used, a worker pulls the sealing cover 18 upwards to separate the first magnetic block 20 from the second magnetic block 19, the thin rod 15 and the cambered block 31 move upwards under the action of the third spring 33, the worker pulls the cambered block 31 upwards to take out the thin rod 15 and the liquid injection pipe 16 completely, then takes out the sampling head 13, and then a proper amount of microbial nucleic acid protection solution is contained in the liquid injection pipe 16, so that the bottom cover 34 is in a blocking state.
After the collection of microorganisms through the sampling head 13 is finished, a worker installs the sampling head 13 and the bottom cover 34 together, penetrates the thin rod 15 and the liquid injection pipe 16 through the third spring 33 and slowly puts down, and at the moment, the third spring 33 supports the thin rod 15; then, the worker pushes the cover 18 against the upper end of the protective case 2, and under the attraction of the first magnetic block 20 and the second magnetic block 19, the cover 18 and the protective case 2 can be mounted together.
At this moment, the arc surface block 31 abuts against the arc-shaped groove 30, when the sealing cover 18 abuts against the protective shell 2, the sealing cover 18 drives the arc surface block 31, the thin rod 15 and the lantern ring 14 to move downwards, firstly, the bottom of the lantern ring 14 abuts against the sampling head 13 and pushes the sampling head 13 to move downwards so as to be separated from the bottom cover 34, after the sampling head 13 falls, the annular concave surface at the bottom of the lantern ring 14 abuts against the bottom cover 34 and is driven to be separated from the liquid injection pipe 16, and at this moment, the microorganism nucleic acid protection liquid drops in the liquid injection pipe 16 fall on the inner bottom of the mixing pipe 12 and can be mixed with collected microorganisms.
Then the staff presses control button 9, and motor 6 work drives cam 7 and rotates, and cam 7 rotates drive arc piece 5 and removes, under the effect of first spring 4, can realize that arc piece 5 resets to realize that arc piece 5, supporting shoe 11, mixing tube 12 use turning block 25 as axle center reciprocating swing, thereby can realize that sample and microorganism nucleic acid protection liquid intensive mixing and misce bene.
The mixing pipe 12 and the liquid injection pipe 16 can be protected by the protective shell 2, so that the mixing pipe and the liquid injection pipe are not easy to damage in carrying and transportation, and meanwhile, the sample is not easy to be influenced by the external environment in a closed environment when being mixed with the microbial nucleic acid protective liquid by the sealing cover 18 and the protective shell 2, so that the sample can be protected more conveniently.
When the mixing tube 12 needs to be taken out, the mixing tube 12 is moved upwards to be separated from the arc-shaped plate 17, and then the mixed sample can be taken out by opening the plug 23.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. The collection and protection device for the microbial nucleic acid sample comprises a sampling head (13) and a protective shell (2), and is characterized in that a supporting seat (1) is fixedly installed at the bottom of the protective shell (2), an arc-shaped rod (3) is fixedly connected to the inner wall of the protective shell (2), an arc-shaped block (5) in sliding connection with the arc-shaped rod (3) is sleeved on the arc-shaped rod (3), first springs (4) are fixedly connected between two sides of the arc-shaped block (5) and the inner wall of the protective shell (2), the two first springs (4) are sleeved outside the arc-shaped rod (3), a driving mechanism is arranged between the arc-shaped block (5) and the inner bottom of the protective shell (2), a supporting block (11) is fixedly connected to the upper end of the arc-shaped block (5), a mixing tube (12) is arranged above the supporting block (11), and an injection tube (22) is installed at the bottom of the mixing tube (12), the injection device is characterized in that a plug (23) is installed at the bottom of the injection tube (22), a limiting mechanism for limiting the mixing tube (12) and the injection tube (22) is arranged at the upper end of the supporting block (11), two arc-shaped plates (17) are arranged in the protective shell (2), the two arc-shaped plates (17) are abutted against the outer wall of the mixing tube (12), abutting mechanisms are arranged between the back surfaces of the two arc-shaped plates (17) and the inner wall of the protective shell (2), the sampling head (13) is positioned in the mixing tube (12), an injection tube (16) is arranged in the mixing tube (12), two supporting rods (10) are fixedly connected to the outer wall of the injection tube (16), the two supporting rods (10) are abutted against the upper end of the mixing tube (12), a bottom cover (34) which cannot be separated is installed at the bottom of the injection tube (16), and the sampling head (13) is installed at the bottom of the bottom cover (34), the upper end of protective housing (2) is equipped with closing cap (18), be equipped with fixed establishment between closing cap (18) and protective housing (2), the bottom of closing cap (18) is equipped with arc wall (30), be equipped with pushing mechanism between arc wall (30) and notes liquid pipe (16), actuating mechanism is including installing motor (6) of bottom in protective housing (2), the output shaft of motor (6) is fixed with cam (7), cam (7) offset with the outer wall of arc piece (5), power (8) and control button (9) are installed to the outer wall of protective housing (2), motor (6), power (8) and control button (9) electric series.
2. The collection and protection device for the microbial nucleic acid sample according to claim 1, wherein the limiting mechanism comprises a first groove (21) arranged on the support block (11), a second groove (24) is arranged at the bottom of the first groove (21), and the mixing tube (12) and the injection tube (22) respectively abut against the first groove (21) and the second groove (24).
3. The collection and protection device for the microbial nucleic acid sample according to claim 1, wherein the abutting mechanism comprises a rotating block (25) rotatably connected with the inner wall of the protective shell (2), an inner cavity (26) is arranged inside the rotating block (25), a sliding block (28) is slidably connected in the inner cavity (26), a second spring (27) is fixed between the sliding block (28) and the inner wall of the inner cavity (26), a rotating rod (29) is fixedly connected to the other end of the sliding block (28), the rotating rod (29) penetrates through one end side wall of the rotating block (25) and is slidably connected with the same, and the rotating rod (29) is rotatably connected with the outer wall of the arc-shaped plate (17).
4. The collection and protection device for the microbial nucleic acid sample as claimed in claim 1, wherein the fixing mechanism comprises a first magnetic block (20) fixed at the upper end of the protective shell (2), a second magnetic block (19) is fixedly connected to the bottom of the cover (18), the first magnetic block (20) and the second magnetic block (19) are abutted, and the opposite surfaces of the first magnetic block (20) and the second magnetic block (19) have opposite magnetic poles.
5. The collection and protection device for the microbial nucleic acid sample according to claim 1, wherein the pushing mechanism comprises a third spring (33) fixed on the inner wall of the mixing tube (12), the outer wall of the liquid injection tube (16) is sleeved with a lantern ring (14), the upper end of the lantern ring (14) is fixedly connected with two thin rods (15), the liquid injection tube (16) and the two thin rods (15) both penetrate through the third spring (33) and abut against the third spring, the upper ends of the two thin rods (15) are fixedly connected with an arc-shaped block (31) together, the arc-shaped block (31) abuts against the inner wall of the arc-shaped groove (30), and the upper end of the liquid injection tube (16) is fixedly mounted with an annular block (32).
6. The collection and protection device for a microbial nucleic acid sample according to claim 5, wherein the third spring (33) is in a circular truncated cone shape, and the diameter of the upper end of the third spring (33) is smaller than that of the lower end.
7. The collection and protection device for microbial nucleic acid samples according to claim 5, wherein the liquid injection pipe (16) is in an inverted truncated cone shape.
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| CN202110531379.9A CN112980663B (en) | 2021-05-17 | 2021-05-17 | A collection and protection device for microorganism nucleic acid sample |
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| CN202110531379.9A CN112980663B (en) | 2021-05-17 | 2021-05-17 | A collection and protection device for microorganism nucleic acid sample |
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| CN112980663B true CN112980663B (en) | 2021-07-23 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114176648B (en) * | 2022-01-11 | 2023-08-01 | 内蒙古民族大学 | Stomach microorganism sampling device for animals |
| CN116650028B (en) * | 2023-07-20 | 2023-10-10 | 山东君泰安德医疗科技股份有限公司 | saliva collector |
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