CN112979383A - Bio-organic fertilizer for promoting crop growth and preparation method and application thereof - Google Patents
Bio-organic fertilizer for promoting crop growth and preparation method and application thereof Download PDFInfo
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- CN112979383A CN112979383A CN202110382514.8A CN202110382514A CN112979383A CN 112979383 A CN112979383 A CN 112979383A CN 202110382514 A CN202110382514 A CN 202110382514A CN 112979383 A CN112979383 A CN 112979383A
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- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 116
- 230000012010 growth Effects 0.000 title claims abstract description 24
- 230000001737 promoting effect Effects 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title abstract description 13
- 239000003337 fertilizer Substances 0.000 claims abstract description 76
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 34
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 34
- 241000563903 Bacillus velezensis Species 0.000 claims abstract description 34
- 241000193417 Brevibacillus laterosporus Species 0.000 claims abstract description 34
- 244000005700 microbiome Species 0.000 claims abstract description 34
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 25
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 239000011573 trace mineral Substances 0.000 claims abstract description 21
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 21
- 150000001875 compounds Chemical class 0.000 claims abstract description 19
- 239000002131 composite material Substances 0.000 claims abstract description 11
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical group [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 46
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 23
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- 235000007079 manganese sulphate Nutrition 0.000 claims description 23
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 23
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 22
- 238000002156 mixing Methods 0.000 claims description 15
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- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims description 12
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 claims description 12
- 108010028690 Fish Proteins Proteins 0.000 claims description 12
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims description 12
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 claims description 12
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 claims description 12
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- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 11
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 11
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- JHECKPXUCKQCSH-UHFFFAOYSA-J calcium;disodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate;hydrate Chemical compound O.[Na+].[Na+].[Ca+2].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O JHECKPXUCKQCSH-UHFFFAOYSA-J 0.000 claims description 3
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- 125000005619 boric acid group Chemical group 0.000 claims description 2
- 244000038559 crop plants Species 0.000 claims 1
- 238000011282 treatment Methods 0.000 abstract description 85
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- 235000010799 Cucumis sativus var sativus Nutrition 0.000 abstract description 14
- 229910002651 NO3 Inorganic materials 0.000 abstract description 11
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract description 11
- 239000000126 substance Substances 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- 239000005416 organic matter Substances 0.000 abstract description 3
- 239000000843 powder Substances 0.000 description 49
- 238000000855 fermentation Methods 0.000 description 37
- 230000004151 fermentation Effects 0.000 description 37
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 24
- 239000002994 raw material Substances 0.000 description 18
- 230000000813 microbial effect Effects 0.000 description 16
- 241000196324 Embryophyta Species 0.000 description 15
- 230000000694 effects Effects 0.000 description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 12
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- 239000007864 aqueous solution Substances 0.000 description 12
- 229910000019 calcium carbonate Inorganic materials 0.000 description 12
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 12
- 239000008103 glucose Substances 0.000 description 12
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- 230000001580 bacterial effect Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
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- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 102000030523 Catechol oxidase Human genes 0.000 description 1
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- 108010052832 Cytochromes Proteins 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 244000000005 bacterial plant pathogen Species 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 238000004178 biological nitrogen fixation Methods 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
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- 210000003608 fece Anatomy 0.000 description 1
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- 108010057270 haemoferritin Proteins 0.000 description 1
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 229910001437 manganese ion Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
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- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D5/00—Fertilisers containing magnesium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a biological organic fertilizer for promoting crop growth and a preparation method and application thereof, and relates to the technical field of biological organic fertilizers, wherein the biological organic fertilizer comprises the following components in parts by weight: 6-15 parts of compound microorganism, 75-85 parts of organic fertilizer, 5-10 parts of medium element fertilizer and 1-2 parts of trace element fertilizer; the composite microorganisms comprise brevibacillus laterosporus, bacillus methylotrophicus and bacillus subtilis, and the effective viable bacteria content of the composite microorganisms in the biological organic fertilizer is more than 30 hundred million cfu/g. On the basis of conventional fertilization, 150kg of the biological organic fertilizer is applied to each mu, the organic matter content of the soil is increased, the nitrate content is obviously reduced, and the physical and chemical properties are improved; the cucumber plant grows well, and the morbidity is reduced; the cucumber yield is obviously higher than that of the conventional fertilization treatment and the control without applying any fertilizer, and the economic benefit is remarkably increased.
Description
Technical Field
The invention relates to the technical field of biological organic fertilizers, in particular to a biological organic fertilizer for promoting the growth of crops and a preparation method and application thereof.
Background
Agriculture is the foundation of national economy, and guaranteeing national food safety and effective supply of important agricultural products are the primary tasks for building modern agriculture. The fertilizer plays a vital role as the most basic agricultural production data. In recent years, in order to pursue high yield of agricultural products, chemical fertilizers are excessively applied by growers, the physical and chemical properties of soil are changed due to excessive nutrient supply, the content of organic matters is reduced, the microbial community of the soil is unbalanced, the number of harmful pathogenic bacteria is increased rapidly, the biological diversity and effectiveness are reduced, and the problems of soil hardening, salinization, continuous cropping obstacle, frequent crop diseases and other series of agricultural planting are increasingly severe.
The plant pathogenic bacteria in the soil are affected by host plants and environmental conditions in the processes of growth, development and pathogenesis, a method for controlling the occurrence and development of diseases by utilizing beneficial microorganisms is called as plant disease biological control, and the beneficial microorganisms are called as plant disease biocontrol bacteria.
The biological organic fertilizer is a functional fertilizer prepared by taking livestock and poultry manure, crop straws, agricultural and sideline products, municipal sludge, solid organic waste materials of food processing and the like as raw materials and mixing with a plurality of beneficial microbial agents. Compared with inorganic fertilizers, the biological organic fertilizer integrates the advantages of traditional organic fertilizers and microbial fertilizers, can effectively improve the utilization rate of the fertilizer, improve the soil quality and increase the quantity of beneficial microorganisms in the soil, and is very important for the development of green organic agriculture.
The action strength and the number of viable bacteria of probiotics contained in the biological organic fertilizer determine the quality of the biological organic fertilizer, strains with strong activity, high temperature resistance, high permeability, drought resistance and the like are generally used in the production of the current biological organic fertilizer, but the number of the strains is generally not high, the dosage form is mainly solid powder or particles, and the strains are generally applied as base fertilizers along with base fertilizers.
Disclosure of Invention
In view of the above, the invention aims to provide a bio-organic fertilizer for promoting crop growth, and a preparation method and an application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a biological organic fertilizer for promoting crop growth, which comprises the following components in parts by weight:
6-15 parts of compound microorganism, 75-85 parts of organic fertilizer, 5-10 parts of medium element fertilizer and 1-2 parts of trace element fertilizer;
the composite microorganisms are Brevibacillus laterosporus, Bacillus methylotrophicus and Bacillus subtilis, the effective viable bacteria content of the composite microorganisms in the bio-organic fertilizer is more than 30 hundred million cfu/g, and the effective viable bacteria content of each Bacillus in the bio-organic fertilizer is more than 2 hundred million cfu/g.
Preferably, the mass ratio of the brevibacillus laterosporus to the bacillus methylotrophicus to the bacillus subtilis is 2-5:2-5: 2-5.
Preferably, the organic fertilizer is fish protein, fulvic acid and rhamnose, and the mass ratio of the fish protein to the fulvic acid to the rhamnose is 5-10:65-75: 5-15.
Preferably, the secondary element fertilizer is magnesium sulfate, EDTA-Ca and calcium nitrate, and the mass ratio of the magnesium sulfate to the EDTA-Ca to the calcium nitrate is 2.5-5:1-3: 1.5-5.
Preferably, the trace element fertilizer is boric acid, manganese sulfate, zinc sulfate and ferrous sulfate, and the mass ratio of the boric acid to the manganese sulfate to the zinc sulfate to the ferrous sulfate is 0.2-0.5:0.15-0.5:0.2-0.5: 0.15-0.5.
The invention also provides a preparation method of the bio-organic fertilizer, which comprises the following steps: and mixing the compound microorganism, the organic fertilizer, the secondary element fertilizer and the trace element fertilizer to obtain the biological organic fertilizer.
The invention also provides application of the bio-organic fertilizer in the technical scheme in promoting crop growth.
The invention also provides application of the bio-organic fertilizer in the technical scheme in reducing disease incidence of crops.
Preferably, the crop comprises cucumber.
Preferably, the application comprises: the dosage of the biological organic fertilizer is 150 kg/mu.
The invention provides a biological organic fertilizer for promoting crop growth, and a preparation method and application thereof, wherein the biological organic fertilizer comprises the following components in parts by weight: 6-15 parts of compound microorganism, 75-85 parts of organic fertilizer, 5-10 parts of medium element fertilizer and 1-2 parts of trace element fertilizer; the composite microorganisms comprise brevibacillus laterosporus, bacillus methylotrophicus and bacillus subtilis, and the effective viable bacteria content of the composite microorganisms in the biological organic fertilizer is more than 30 hundred million cfu/g. The biological organic fertilizer provided by the invention contains high biocontrol bacteria viable count, and after being applied to soil, the biological organic fertilizer can restore the microbial community structure of the soil, supplement the organic matter content of the soil, reduce the disease incidence of plant diseases, improve the utilization rate of the fertilizer and promote the growth of crops.
Detailed Description
The invention provides a biological organic fertilizer for promoting crop growth, which comprises the following components in parts by weight:
6-15 parts of compound microorganism, 75-85 parts of organic fertilizer, 5-10 parts of medium element fertilizer and 1-2 parts of trace element fertilizer;
the composite microorganisms are Brevibacillus laterosporus, Bacillus methylotrophicus and Bacillus subtilis, the effective viable bacteria content of the composite microorganisms in the bio-organic fertilizer is more than 30 hundred million cfu/g, and the effective viable bacteria content of each Bacillus in the bio-organic fertilizer is more than 2 hundred million cfu/g.
The biological organic fertilizer provided by the invention comprises 6-15 parts by weight of composite microorganisms, preferably 10 parts by weight. In the present invention, the mass ratio of Brevibacillus laterosporus, Bacillus methylotrophicus and Bacillus subtilis is preferably 2-5:2-5: 2-5. The sources of the brevibacillus laterosporus, the bacillus methylotrophicus and the bacillus subtilis are not specially limited, and the bacillus laterosporus, the bacillus methylotrophicus and the bacillus subtilis can be obtained by adopting conventional commercially available strains. According to the invention, brevibacillus laterosporus, bacillus methylotrophicus and bacillus subtilis are selected and respectively fermented independently, and the powder raw materials with high purity and high bacterial count are prepared respectively in a powder spraying manner, and meanwhile, a plurality of effective metabolites in the fermentation liquor are reserved. After the fertilizer is applied to soil, functional microorganisms propagate and grow in a large quantity, the number and the structure of rhizosphere soil microbial communities can be improved, the soil microbial biomass is increased, and the diversity of soil microorganisms is improved; the dominant flora is formed at the rhizosphere of the crops, so that the growth and the propagation of other harmful microorganisms are inhibited, the antagonism on harmful pathogenic bacteria is generated, and the occurrence of diseases is reduced; the functional microorganism can induce crops to generate peroxidase, polyphenol oxidase, glucanase and the like to participate in the defense reaction of plants to harmful microorganisms, and the adaptability of the crops to adverse environments is improved.
The biological organic fertilizer provided by the invention comprises 75-85 parts of organic fertilizer by weight, and preferably 78-80 parts. In the invention, the organic fertilizer is preferably fish protein, fulvic acid and rhamnose, and the mass ratio of the fish protein to the fulvic acid and the rhamnose is preferably 5-10:65-75: 5-15. In the invention, after the biological organic fertilizer is applied to soil, fulvic acid can improve the content of organic matters in the soil, promote the increase of a soil granular structure, the reduction of soil volume weight and the increase of porosity, and is beneficial to water saving and fertilizer conservation of the soil; besides providing nutrients for promoting the rapid colonization of the thallus superiority, the fish protein and rhamnose can also provide long-acting nutrients for crops and improve the quality of the crops.
The biological organic fertilizer provided by the invention comprises 5-10 parts of medium element fertilizer by weight, and preferably 8-8.5 parts. In the invention, the medium element fertilizer preferably comprises magnesium sulfate, EDTA-Ca and calcium nitrate, and the mass ratio of the magnesium sulfate, the EDTA-Ca and the calcium nitrate is preferably 2.5-5:1-3: 1.5-5. In the invention, calcium ions in the medium element fertilizer can stabilize the structure of crop cell membranes, stimulate cell elongation and accelerate root growth and development; the magnesium ions can enhance the photosynthesis of crops, activate key enzymes of physiological metabolism in the crops and promote the growth and development of the crops.
The biological organic fertilizer provided by the invention comprises 1-2 parts of trace element fertilizer by weight, and preferably 1.5 parts. In the invention, the trace element fertilizer preferably comprises boric acid, manganese sulfate, zinc sulfate and ferrous sulfate, and the mass ratio of the boric acid to the manganese sulfate to the zinc sulfate to the ferrous sulfate is preferably 0.2-0.5:0.15-0.5:0.2-0.5: 0.15-0.5. In the invention, iron ions in the trace element fertilizer are important components of cytochrome and non-heme ferritin and participate in photosynthesis, biological nitrogen fixation and respiration; manganese ions act as activators of many enzymes in the cell, affecting glycolysis and the tricarboxylic acid cycle; boron is a constituent of cell wall hemicellulose; zinc is an essential element of chlorophyll plants. The addition of the trace elements can stabilize the normal physiological metabolism and decomposition function of plants, promote the growth and development of the plants and avoid the problems of poor growth and development, yield reduction, quality reduction and the like caused by deficiency in elements in the later growth stage of plants.
In the invention, the total content of microorganisms in the biological organic fertilizer is more than or equal to 30 hundred million cfu/g, and the content of organic matters is more than or equal to 40 percent.
The invention also provides a preparation method of the bio-organic fertilizer, which comprises the following steps: and mixing the compound microorganism, the organic fertilizer, the secondary element fertilizer and the trace element fertilizer to obtain the biological organic fertilizer.
In the invention, the brevibacillus laterosporus, the bacillus methylotrophicus and the bacillus subtilis in the compound microorganism are preferably prepared into bacterial powder and mixed to obtain the compound microorganism.
In the present invention, the method for preparing the brevibacillus laterosporus powder preferably comprises: inoculating the brevibacillus laterosporus into a fermentation tank aqueous solution containing 0.5% of glucose, 2.0% of starch, 1.5% of bean cake powder, 1% of yeast powder, 60.2% of magnesium sulfate, 0.3% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate and 0.03% of manganese sulfate and having the pH value of 7.0-7.2 at the temperature of 121 ℃ after sterilization for 30min, and fermenting for 48-72h to obtain brevibacillus laterosporus fermentation liquor; adding a carrier according to the proportion of adding 5-10kg of the carrier into each ton of fermentation liquor, and spraying and drying to prepare the brevibacillus laterosporus powder, wherein the viable bacteria content of the brevibacillus laterosporus in the powder is more than 500 hundred million cfu/g. The type of the vector is not particularly limited, and the vector can be a conventional vector for adsorbing the brevibacillus laterosporus.
In the present invention, the preparation method of the bacillus methylotrophicus powder preferably comprises: inoculating bacillus methylotrophicus into a fermentation tank aqueous solution containing 1% of glucose, 1.5% of starch, 2.0% of bean cake powder, 1% of yeast powder, 0.3% of magnesium sulfate, 0.3% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate and 0.03% of manganese sulfate and having a pH value of 7.0-7.2 at a strain inoculation amount of 2.0-3.0% (g/L) at 121 ℃, sterilizing for 30min, cooling to 34-37 ℃, and fermenting for 48-72h to obtain bacillus methylotrophicus fermentation liquor; adding a carrier according to the proportion of adding 5-10kg of the carrier into each ton of fermentation liquor, and spraying and drying to prepare the bacillus methylotrophicus powder, wherein the viable bacteria content of the bacillus methylotrophicus in the powder is more than 100 hundred million cfu/g. The type of the vector is not particularly limited, and the vector can be a conventional vector for adsorbing the bacillus methylotrophicus.
In the present invention, the preparation method of the bacillus subtilis powder preferably comprises: inoculating bacillus subtilis according to the inoculation amount of 1.5-3.0% (g/L) into a fermentation tank aqueous solution containing 1.5% of glucose, 2% of starch, 2% of bean cake powder, 1% of yeast powder, 0.5% of magnesium sulfate, 0.5% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.05% of manganese sulfate and the pH value of 7.0-7.2 after sterilizing at 121 ℃ for 30min, and fermenting for 48-72h to obtain bacillus subtilis fermentation liquor; adding a carrier according to the proportion of adding 5-10kg of the carrier into each ton of fermentation liquor, and spraying and drying to prepare the bacillus subtilis powder, wherein the viable bacteria content of the bacillus subtilis in the powder is more than 500 hundred million cfu/g. The invention has no special limitation on the types of the vectors, and the vectors can be used for adsorbing the bacillus subtilis.
In the invention, the raw materials of the organic fertilizer, the secondary element fertilizer and the trace element fertilizer are preferably crushed, sieved by a 100-mesh sieve and mixed with undersize products. In the invention, the particle size of the bio-organic fertilizer is preferably less than 100 meshes. The biological organic fertilizer adopts a full water-soluble formula, can be applied along with water besides bottom application, is convenient for water conservation and emission reduction, and improves the absorption utilization rate of the fertilizer.
The invention also provides application of the bio-organic fertilizer in the technical scheme in promoting crop growth.
The invention also provides application of the bio-organic fertilizer in the technical scheme in reducing disease incidence of crops.
In the present invention, the crop preferably comprises cucumber.
In the present invention, the application preferably includes: the dosage of the biological organic fertilizer is 150 kg/mu. The application method of the biological organic fertilizer is not particularly limited, and the application method of the conventional biological organic fertilizer is adopted.
In order to further illustrate the present invention, the following detailed description of the invention is given in conjunction with examples, which should not be construed to limit the scope of the invention.
Example 1
1. A bio-organic fertilizer for promoting crop growth comprises the following raw materials in percentage by weight: 10% of compound microbial agent, 79% of organic fertilizer, 10% of secondary element fertilizer and 1% of trace element fertilizer.
Wherein the compound microbial agent accounts for the following weight percentages of the biological organic fertilizer: 3% of brevibacillus laterosporus, 3% of bacillus methylotrophicus and 4% of bacillus subtilis;
wherein the organic fertilizer accounts for the weight percentage of the biological organic fertilizer: 4% of fish protein, 70% of fulvic acid and 5% of rhamnose;
wherein the secondary element fertilizer accounts for the weight percentage of the biological organic fertilizer: 5% magnesium sulfate, 2% EDTA-Ca, 3% calcium nitrate;
wherein the microelement fertilizer accounts for the weight percentage of the biological organic fertilizer: 0.25% boric acid, 0.25% manganese sulfate, 0.25% zinc sulfate, 0.25% ferrous sulfate;
the total content of the microorganisms is 33.5 hundred million cfu/g, and the content of organic matters is 45.5 percent.
2. The preparation method comprises the following steps:
step one, inoculating brevibacillus laterosporus into a fermentation tank aqueous solution containing 0.5% of glucose, 2.0% of starch, 1.5% of bean cake powder, 1% of yeast powder, 0.2% of magnesium sulfate, 0.3% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.03% of manganese sulfate and pH 7.0 after sterilizing at 121 ℃ for 30min, and fermenting for 50h to obtain brevibacillus laterosporus fermentation liquor; adding 6.5kg of carrier into each ton of fermentation liquor, adding the carrier, and spray-drying to prepare brevibacillus laterosporus powder, wherein the effective bacteria content of the brevibacillus laterosporus is 515 hundred million cfu/g;
secondly, inoculating bacillus methylotrophicus into a fermentation tank aqueous solution containing 1% of glucose, 1.5% of starch, 2.0% of bean cake powder, 1% of yeast powder, 0.3% of magnesium sulfate, 0.3% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.03% of manganese sulfate and pH 7 after sterilizing at 121 ℃ for 30min, and fermenting for 48 hours to obtain bacillus methylotrophicus fermentation liquor; adding 8kg of carrier into each ton of fermentation liquor, and spraying and drying to prepare bacillus methylotrophicus powder, wherein the effective bacteria content of the bacillus methylotrophicus is 108 hundred million cfu/g;
inoculating bacillus subtilis into a fermentation tank aqueous solution containing 1.5% of glucose, 2% of starch, 2% of bean cake powder, 1% of yeast powder, 0.5% of magnesium sulfate, 0.5% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.05% of manganese sulfate and pH (7.0) according to the inoculation amount of 2.5% (g/L), sterilizing at 121 ℃, cooling to 35 ℃ after 30min, and fermenting for 48h to obtain bacillus subtilis fermentation liquor; adding a carrier according to the proportion of adding 10kg of the carrier into each ton of fermentation liquor, and spraying and drying to prepare bacillus subtilis powder, wherein the effective bacteria content of the bacillus subtilis is 520 hundred million cfu/g;
step four, weighing each bacterial powder raw material of the compound microbial agent prepared in the step one to the step three, and uniformly mixing to prepare a first mixture;
weighing raw materials of the medium element fertilizer and the trace element raw materials, mixing, crushing, sieving by a 100-mesh sieve, adding the first mixture, and fully and uniformly stirring to obtain a second mixture;
and step six, weighing the raw materials of the organic fertilizers, mixing, crushing, sieving by a 100-mesh sieve, adding the second mixture, and fully and uniformly stirring to obtain the biological organic fertilizer.
Example 2
1. A bio-organic fertilizer for promoting crop growth comprises the following raw materials in percentage by weight: 10% of compound microbial agent, 80% of organic fertilizer, 8% of secondary element fertilizer and 2% of trace element fertilizer.
Wherein the compound microbial agent accounts for the following weight percentages of the biological organic fertilizer: 2% of brevibacillus laterosporus, 4% of bacillus methylotrophicus and 4% of bacillus subtilis;
wherein the organic fertilizer accounts for the weight percentage of the biological organic fertilizer: 5% of fish protein, 65% of fulvic acid and 10% of rhamnose;
wherein the secondary element fertilizer accounts for the weight percentage of the biological organic fertilizer: 4% magnesium sulfate, 2% EDTA-Ca, 2% calcium nitrate
Wherein the microelement fertilizer accounts for the weight percentage of the biological organic fertilizer: 0.5% boric acid, 0.5% manganese sulfate, 0.5% zinc sulfate, 0.5% ferrous sulfate
The total content of the microorganisms is 35.2 hundred million cfu/g, and the content of organic matters is 43.3 percent.
2. The preparation method comprises the following steps:
step one, inoculating brevibacillus laterosporus into a fermentation tank aqueous solution containing 0.5% of glucose, 2.0% of starch, 1.5% of bean cake powder, 1% of yeast powder, 0.2% of magnesium sulfate, 0.3% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.03% of manganese sulfate and pH 7.0 after sterilizing at 121 ℃ for 30min, and fermenting for 52h to obtain brevibacillus laterosporus fermentation liquor; adding 6.5kg of carrier into each ton of fermentation liquor, adding the carrier, and spray-drying to prepare brevibacillus laterosporus powder, wherein the effective bacteria content of the brevibacillus laterosporus is 531 hundred million cfu/g;
secondly, inoculating bacillus methylotrophicus into a fermentation tank aqueous solution containing 1% of glucose, 1.5% of starch, 2.0% of bean cake powder, 1% of yeast powder, 0.3% of magnesium sulfate, 0.3% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.03% of manganese sulfate and pH 7 after sterilizing at 121 ℃ for 30min, and fermenting for 50 hours to obtain bacillus methylotrophicus fermentation liquor; adding 8kg of carrier into each ton of fermentation liquor, and spraying and drying to prepare bacillus methylotrophicus powder, wherein the effective bacteria content of the bacillus methylotrophicus is 110 hundred million cfu/g;
inoculating bacillus subtilis into a fermentation tank aqueous solution containing 1.5% of glucose, 2% of starch, 2% of bean cake powder, 1% of yeast powder, 0.5% of magnesium sulfate, 0.5% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.05% of manganese sulfate and pH (7.0) according to the inoculation amount of 3% (g/L), sterilizing at 121 ℃, cooling to 35 ℃ after 30min, and fermenting for 54h to obtain bacillus subtilis fermentation liquor; adding a carrier according to the proportion of adding 10kg of the carrier into each ton of fermentation liquor, and spraying and drying to prepare bacillus subtilis powder, wherein the effective bacteria content of bacillus subtilis is 563 hundred million cfu/g;
step four, weighing each bacterial powder raw material of the compound microbial agent prepared in the step one to the step three, and uniformly mixing to prepare a first mixture;
weighing raw materials of the medium element fertilizer and the trace element raw materials, mixing, crushing, sieving by a 100-mesh sieve, adding the first mixture, and fully and uniformly stirring to obtain a second mixture;
and step six, weighing the raw materials of the organic fertilizers, mixing, crushing, sieving by a 100-mesh sieve, adding the second mixture, and fully and uniformly stirring to obtain the biological organic fertilizer.
Example 3
1. A bio-organic fertilizer for promoting crop growth comprises the following raw materials in percentage by weight: 15% of compound microbial agent, 75% of organic fertilizer, 8.5% of secondary element fertilizer and 1.5% of trace element fertilizer.
Wherein the compound microbial agent accounts for the following weight percentages of the biological organic fertilizer: 5% of Brevibacillus laterosporus, 5% of Bacillus methylotrophicus and 5% of Bacillus subtilis;
wherein the organic fertilizer accounts for the weight percentage of the biological organic fertilizer: 5% of fish protein, 65% of fulvic acid and 5% of rhamnose;
wherein the secondary element fertilizer accounts for the weight percentage of the biological organic fertilizer: 3.5% magnesium sulfate, 2.5% EDTA-Ca, 2.5% calcium nitrate
Wherein the microelement fertilizer accounts for the weight percentage of the biological organic fertilizer: 0.5% boric acid, 0.15% manganese sulfate, 0.35% zinc sulfate, 0.5% ferrous sulfate
The total content of the microorganisms is 42.5 hundred million cfu/g, and the content of organic matters is 42.1 percent.
2. The preparation method comprises the following steps:
step one, inoculating brevibacillus laterosporus into a fermentation tank aqueous solution containing 0.5% of glucose, 2.0% of starch, 1.5% of bean cake powder, 1% of yeast powder, 0.2% of magnesium sulfate, 0.3% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.03% of manganese sulfate and pH 7.0 after sterilizing at 121 ℃ for 30min, and fermenting for 51h to obtain brevibacillus laterosporus fermentation liquor; adding a carrier according to the proportion of adding 7kg of the carrier into each ton of fermentation liquor, and spraying and drying to prepare brevibacillus laterosporus powder, wherein the effective bacteria content of the brevibacillus laterosporus is 519 hundred million cfu/g;
secondly, inoculating 2.0% (g/L) of bacillus methylotrophicus into a fermentation tank aqueous solution containing 1% of glucose, 1.5% of starch, 2.0% of bean cake powder, 1% of yeast powder, 0.3% of magnesium sulfate, 0.3% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.03% of manganese sulfate and the pH value of 7, and fermenting for 49 hours to obtain bacillus methylotrophicus fermentation liquor after sterilizing at 121 ℃ for 30 min; adding 8.3kg of carrier into each ton of fermentation liquor, adding the carrier, and spray-drying to prepare bacillus methylotrophicus powder, wherein the effective bacteria content of the bacillus methylotrophicus is 105 hundred million cfu/g;
inoculating bacillus subtilis into a fermentation tank aqueous solution containing 1.5% of glucose, 2% of starch, 2% of bean cake powder, 1% of yeast powder, 0.5% of magnesium sulfate, 0.5% of calcium carbonate, 0.15% of dipotassium hydrogen phosphate, 0.05% of manganese sulfate and pH (7.0) for 54h after sterilizing at 121 ℃ for 30min, and fermenting to obtain bacillus subtilis fermentation liquor; adding a carrier according to the proportion of adding 9kg of the carrier into each ton of fermentation liquor, and spraying and drying to prepare bacillus subtilis powder, wherein the effective bacteria content of the bacillus subtilis is 545 hundred million cfu/g;
step four, weighing each bacterial powder raw material of the compound microbial agent prepared in the step one to the step three, and uniformly mixing to prepare a first mixture;
weighing raw materials of the medium element fertilizer and the trace element raw materials, mixing, crushing, sieving by a 100-mesh sieve, adding the first mixture, and fully and uniformly stirring to obtain a second mixture;
and step six, weighing the raw materials of the organic fertilizers, mixing, crushing, sieving by a 100-mesh sieve, adding the second mixture, and fully and uniformly stirring to obtain the biological organic fertilizer.
And (3) field test:
first, experimental design
1. Experimental Material
Selecting cucumbers from the biological organic fertilizer prepared in the embodiment 1-3 to perform a field experiment; the test is carried out in the same protected test field, and the variety of the tested cucumber is Guiqing No. 1; the experiment is provided with 7 treatments, each treatment is repeated for 3 times, the random block design is carried out, and the cell area is 15m2And in each season, the seeds are independently harvested, weighed and counted according to the residential area, and other cultivation management measures are consistent with the habits of local farmers.
Treatment 1: and (3) adding the biological organic fertilizer and conventional fertilization, and additionally applying the biological fertilizer prepared in the embodiment 1 of the invention on the basis of the conventional fertilization, wherein the application amount is 150 kg/mu.
And (3) treatment 2: and (3) adding the biological organic fertilizer and conventional fertilization, and additionally applying the biological fertilizer prepared in the embodiment 2 of the invention on the basis of the conventional fertilization, wherein the application amount is 150 kg/mu.
And (3) treatment: and (3) adding the biological organic fertilizer and conventional fertilization, and additionally applying the biological fertilizer prepared in the embodiment 3 of the invention on the basis of the conventional fertilization, wherein the application amount is 150 kg/mu.
And (4) treatment: and (2) adding a biological organic fertilizer and conventional fertilization (the biological organic fertilizer comprises 5% of brevibacillus laterosporus, 5% of bacillus methylotrophicus, 5% of bacillus subtilis, 75% of fulvic acid, 3.5% of magnesium sulfate, 2.5% of EDTA-Ca, 2.5% of calcium nitrate, 0.5% of boric acid, 0.15% of manganese sulfate, 0.35% of zinc sulfate and 0.5% of ferrous sulfate), and additionally applying 150 kg/mu of the biological organic fertilizer on the basis of the conventional fertilization.
And (4) treatment 5: the organic fertilizer and conventional fertilizer application (the organic fertilizer comprises 75% of fulvic acid, 7.5% of fish protein, 7.5% of rhamnose, 3.5% of magnesium sulfate, 2.5% of EDTA-Ca, 2.5% of calcium nitrate, 0.5% of boric acid, 0.15% of manganese sulfate, 0.35% of zinc sulfate and 0.5% of ferrous sulfate) are carried out, and 150 kg/mu of the organic fertilizer is additionally applied on the basis of the conventional fertilizer application.
6, treatment: applying fertilizer, applying potassium sulfate compound fertilizer (N: P)2O5:K2O15: 15:15)60 kg/mu.
And (7) treatment: blank control (CK does not apply any fertilizer to the water)
The test site is in Shuangjing Zhengshuangjing village of Shuangjing in TieLing City of Liaoning province, the test time is 5 months to 7 months in 2020, the test field is convenient to drain and irrigate, and no fertilizer test is performed in recent years. The physical and chemical indexes of the soil are shown in Table 1
2. Experimental methods
The soil samples are collected before fertilizer application, after cucumber seedling pulling and after crop harvesting, and the crop root system samples are taken for 4 times. Collecting rhizosphere soil samples by adopting a balance jump method, repeatedly positioning 9 sampling points each, taking 1000ml of soil at each sampling point with the sampling depth of 0-25cm, mixing, and reducing the volume to 500ml for standby inspection by using a quartering method.
TABLE 1 basic conditions of the soil of the test plots
Second, experimental results
1. Influence of different fertilization treatments on physicochemical properties of salinized soil of protected land
TABLE 2 Effect of different fertilisation treatments on soil nutrient content
As can be seen from Table 2, when the bio-organic fertilizer prepared in the embodiments 1 to 3 of the invention is used for improving saline soil, compared with CK, the conductivity of the bio-organic fertilizer prepared in the embodiments 1 to 3 is respectively reduced by 0.91ms/kg, 0.88ms/kg and 0.92 ms/kg; the total nitrogen of the soil is respectively increased by 0.21g/kg, 0.16g/kg and 0.14 g/kg; the quick-acting phosphorus is respectively increased by 20.9mg/kg, 19.41mg/kg and 17.10 mg/kg; the quick-acting potassium is increased by 34.11mg/kg, 25.23mg/kg and 23.57 mg/kg; the organic matters of the soil are respectively increased by 7.14g/kg, 6.24g/kg and 5.79 g/kg.
Compared with CK, the conductivity of the treatment 4 and the treatment 5 is respectively reduced by 0.90ms/kg and 0.25 ms/kg; the total nitrogen of the soil is respectively increased by 0.08g/kg and 0.28 g/kg; the quick-acting phosphorus is respectively increased by 12.44mg/kg and 8.68 mg/kg; the quick-acting potassium is increased by 18.9mg/kg and 19.89 mg/kg; the organic matters of the soil are respectively increased by 6.13g/kg and 9.76 g/kg; although it is improved compared with CK, it is compared with the bio-organic fertilizer of this application.
Comprehensive comparison shows that the conductivity, the quick-acting phosphorus and the quick-acting potassium of the treatment 1-treatment 3 are superior to those of the treatment 4 and the treatment 5, and are obviously superior to those of the treatment 6 and CK, so that the organic fertilizer disclosed by the invention achieves a prominent effect through organic combination of specific components, and the shortage or lack of a certain component can not exert the most prominent effect; therefore, the application of the bio-organic fertilizer prepared in the embodiments 1 to 3 of the invention can improve the physical and chemical properties of soil and improve the soil quality.
2. Influence of different fertilization treatments on nitrate content of salinized soil in protected land
TABLE 3 Effect of different fertilisation treatments on the nitrate content of the soil
As can be seen from table 3, the soil nitrate content after the cucumbers of the control group (CK) and the cucumbers of the treatment 5 and the treatment 6 are harvested is higher than that before the treatment, which indicates that the soil nitrate content without the application of the bio-organic fertilizer is accumulated much; after the soil nitrate is harvested, the content of the soil nitrate is obviously lower than that of the soil nitrate before the soil nitrate is harvested in the treatment 1, the treatment 2, the treatment 3 and the treatment 4, which are respectively reduced by 54.96%, 53.52%, 51.21% and 24.45%, and the treatment 1 to the treatment 3 are obviously better than the treatment 4, which shows that the content of the soil nitrate can be obviously reduced by applying the biological organic fertilizer prepared in the embodiment 1 to the embodiment 3 of the invention.
3. Effect of different fertilization treatments on biological traits of cucumber
TABLE 4 Effect of different fertilisation treatments on biological traits of cucumber
As can be seen from Table 4, the treatments (treatment 1-treatment 3) applying the bio-organic fertilizer prepared in examples 1-3 of the present invention can be used for improving the protected secondary salinized soil, and the cucumber plant characteristics are improved. The plant leaves are dark green, the growth and development are normal, and the saline-alkali harm symptom is not generated. The root knot ratio is significantly reduced, and the treatment 1 to the treatment 3 are respectively reduced by 15.2%, 15.1% and 15.3% compared with the control. Treatment 4 and treatment 5 were reduced by 11.8% and 8.0%, respectively, compared to the control. In comprehensive comparison, treatments 1-3 are significantly superior to treatments 4 and 5.
4. Effect of different fertilization treatments on cucumber plant morbidity
TABLE 5 Effect of different fertilization treatments on cucumber plant morbidity
| Treatment of | Investigating plant number (plant) | Number of plants affected (plant) | Incidence (%) |
| Process 1 | 234 | 30 | 12.82 |
| Treatment 2 | 234 | 33 | 14.10 |
| Treatment 3 | 234 | 24 | 10.25 |
| Treatment 4 | 234 | 40 | 19.09 |
| Treatment 5 | 234 | 42 | 21.95 |
| Treatment 6 | 234 | 69 | 29.48 |
| CK | 234 | 60 | 25.64 |
As can be seen from Table 5, the biological organic fertilizer applied in treatment 1-treatment 3 has better control effect on cucumber diseases, and compared with treatment 6, the morbidity is respectively reduced by 12.66%, 15.38% and 19.23%; the incidence was reduced by 12.82%, 11.54%, 15.39% compared to Control (CK), respectively. Compared with treatment 6, the incidence rates of treatment 4 and treatment 5 are respectively reduced by 10.39% and 7.53%; the incidence was reduced by 6.55% and 3.69% respectively compared to Control (CK). In comprehensive comparison, treatments 1 to 3 were superior to treatments 4 and 5. The biological organic fertilizer prepared in the embodiments 1 to 3 of the invention has a good effect of preventing and treating cucumber diseases, after beneficial microorganisms in the fertilizer are applied to soil, nutrients such as fish protein and rhamnose in the fertilizer are utilized to carry out rapid propagation, and an advantageous flora is established, so that the microbial flora structure in the rhizosphere soil of crops is improved, the propagation of soil-borne pathogenic bacteria is inhibited, the soil environment is optimized, the growth of the root system of the crops is promoted, cucumber plants grow robustly, and the resistance of the plants to the diseases is further improved.
5. Effect of different fertilization treatments on cucumber yield
TABLE 6 Effect of different fertilization treatments on cucumber yield
As can be seen from Table 6: compared with CK, the yield of the treated product 1-treated product 3 is increased by 2359.14 kg/mu, 2024.51 kg/mu and 2146.27 kg/mu respectively, and the yield is increased by 30.58%, 26.24% and 27.82% respectively; compared with treatment 6, treatment 1-treatment 3 respectively increase the yield by 900.35 kg/mu, 656.72 kg/mu and 687.48 kg/mu, and the yield increase rate is respectively 9.81%, 6.17% and 7.49%. Compared with CK, the yield of the treated products is increased by 1950.93 kg/mu and 1855.90 kg/mu respectively, and the yield is increased by 25.28 percent and 24.05 percent respectively in the treatment 4 and the treatment 5; compared with the treatment 6, the treatment 4 and the treatment 5 respectively increase the yield by 492.14 kg/mu and 397.11 kg/mu, and the yield increase rate is 5.36 percent and 4.33 percent. The comprehensive comparison shows that the treatments 1 to 3 are superior to the treatments 4 and 5, and are obviously superior to the control. The data show that the application of the biological organic fertilizer prepared in the embodiments 1 to 3 can effectively improve the acre yield of the cucumbers.
6. Influence of different fertilization treatments on economic benefits
TABLE 7 analysis table for input and output of different fertilization treatments
Note: cucumber is calculated according to 3.5 yuan/kg.
As can be seen from table 7, the net gains of treatments 1-3 were 2861.22 yuan/mu, 2008.52 yuan/mu, and 2116.18 yuan/mu, respectively, compared to treatment 6; compared with CK, the pure income increase of the treatment 1 to the treatment 3 is 7966.99 yuan/mu, 6795.78 yuan/mu and 7221.95 yuan/mu respectively. Compared with the treatment 4 and the treatment 5, the pure income increase is 1452.49 yuan/mu and 1149.89 yuan/mu respectively; compared with CK, the pure income increase of the treatment 4 and the treatment 5 is 6558.26 yuan/mu and 6255.65 yuan/mu respectively. Compared in a comprehensive mode, the treatment 1-treatment 3 is obviously superior to the treatment 4 and the treatment 5, and the fact that the biological organic fertilizer prepared in the embodiment 1-the embodiment 3 of the invention can effectively improve the cucumber yield and the income of farmers by additionally applying the biological organic fertilizer on the basis of conventional fertilization is shown.
According to the comprehensive evaluation of all the test data and the benefit conditions, 150kg of the bio-organic fertilizer prepared by mixing the specific components prepared in the embodiments 1 to 3 of the invention and a specific proportion is applied to each mu on the basis of conventional fertilization, the organic matter content of the soil is increased, the nitrate content is obviously reduced, and the physical and chemical properties are improved; the cucumber plant grows well, and the morbidity is reduced; the cucumber yield is obviously higher than that of the conventional fertilization treatment and the control without applying any fertilizer, and the economic benefit is remarkably increased.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (10)
1. A biological organic fertilizer for promoting the growth of crops is characterized in that according to parts by weight,
6-15 parts of compound microorganism, 75-85 parts of organic fertilizer, 5-10 parts of medium element fertilizer and 1-2 parts of trace element fertilizer;
the composite microorganisms are Brevibacillus laterosporus, Bacillus methylotrophicus and Bacillus subtilis, the effective viable bacteria content of the composite microorganisms in the bio-organic fertilizer is more than 30 hundred million cfu/g, and the effective viable bacteria content of each Bacillus in the bio-organic fertilizer is more than 10 hundred million cfu/g.
2. The bio-organic fertilizer according to claim 1, wherein the mass ratio of the brevibacillus laterosporus, the bacillus methylotrophicus and the bacillus subtilis is 2-5:2-5: 2-5.
3. The bio-organic fertilizer as claimed in claim 1, wherein the organic fertilizer is fish protein, fulvic acid and rhamnose, and the mass ratio of the fish protein to the fulvic acid to the rhamnose is 5-10:65-75: 5-15.
4. The bio-organic fertilizer according to claim 1, wherein the secondary element fertilizer is magnesium sulfate, EDTA-Ca and calcium nitrate, and the mass ratio of the magnesium sulfate, the EDTA-Ca and the calcium nitrate is 2.5-5:1-3: 1.5-5.
5. The bio-organic fertilizer as claimed in claim 1, wherein the trace element fertilizer is boric acid, manganese sulfate, zinc sulfate and ferrous sulfate, and the mass ratio of the boric acid, the manganese sulfate, the zinc sulfate and the ferrous sulfate is 0.2-0.5:0.15-0.5:0.2-0.5: 0.15-0.5.
6. A method for preparing a bio-organic fertilizer according to any one of claims 1 to 5, characterized by comprising the following steps: and mixing the compound microorganism, the organic fertilizer, the secondary element fertilizer and the trace element fertilizer to obtain the biological organic fertilizer.
7. Use of the bioorganic fertilizer of any one of claims 1-5 for promoting the growth of crops.
8. Use of the bio-organic fertilizer of any one of claims 1-5 for reducing the incidence of crop disease.
9. Use according to claim 7 or 8, wherein the crop plants comprise cucumbers.
10. The application according to claim 7 or 8, characterized in that it comprises: the dosage of the biological organic fertilizer is 150 kg/mu.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110382514.8A CN112979383A (en) | 2021-04-09 | 2021-04-09 | Bio-organic fertilizer for promoting crop growth and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113788723A (en) * | 2021-10-29 | 2021-12-14 | 河北馨仙养殖有限公司 | Fruit tree total-nutrient bio-organic fertilizer, preparation method and application thereof |
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| KR101010762B1 (en) * | 2009-10-29 | 2011-01-25 | 지앙수 뉴 그라운드 바이오퍼틸라이저 엔지니어링 센터 컴퍼니 리미티드 | Biologically Controlled Strains and Their Microbial Organic Fertilizers for Withering Disease of Cucumber and Watermelon |
| CN112409101A (en) * | 2020-11-20 | 2021-02-26 | 内蒙古丰沃达生物科技有限公司 | Biological organic fertilizer and preparation method thereof |
| CN112694370A (en) * | 2021-02-01 | 2021-04-23 | 延边兴林生物科技有限公司 | Bio-organic fertilizer for improving stress resistance of crops and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101010762B1 (en) * | 2009-10-29 | 2011-01-25 | 지앙수 뉴 그라운드 바이오퍼틸라이저 엔지니어링 센터 컴퍼니 리미티드 | Biologically Controlled Strains and Their Microbial Organic Fertilizers for Withering Disease of Cucumber and Watermelon |
| CN112409101A (en) * | 2020-11-20 | 2021-02-26 | 内蒙古丰沃达生物科技有限公司 | Biological organic fertilizer and preparation method thereof |
| CN112694370A (en) * | 2021-02-01 | 2021-04-23 | 延边兴林生物科技有限公司 | Bio-organic fertilizer for improving stress resistance of crops and preparation method and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113788723A (en) * | 2021-10-29 | 2021-12-14 | 河北馨仙养殖有限公司 | Fruit tree total-nutrient bio-organic fertilizer, preparation method and application thereof |
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Application publication date: 20210618 |