CN112978904A - Preparation method of biological filler for treating water pollution - Google Patents
Preparation method of biological filler for treating water pollution Download PDFInfo
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- CN112978904A CN112978904A CN202110388030.4A CN202110388030A CN112978904A CN 112978904 A CN112978904 A CN 112978904A CN 202110388030 A CN202110388030 A CN 202110388030A CN 112978904 A CN112978904 A CN 112978904A
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- thallus
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- biological filler
- filler
- water pollution
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- 239000000945 filler Substances 0.000 title claims abstract description 63
- 238000003911 water pollution Methods 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000001580 bacterial effect Effects 0.000 claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 22
- 241001052560 Thallis Species 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 11
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 241001467578 Microbacterium Species 0.000 claims abstract description 7
- 235000019353 potassium silicate Nutrition 0.000 claims abstract description 7
- 238000002791 soaking Methods 0.000 claims abstract description 3
- 241000894006 Bacteria Species 0.000 claims description 14
- 239000005995 Aluminium silicate Substances 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 235000012211 aluminium silicate Nutrition 0.000 claims description 8
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 8
- 229910021536 Zeolite Inorganic materials 0.000 claims description 4
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 4
- 239000010457 zeolite Substances 0.000 claims description 4
- 241000752227 Zobellella Species 0.000 claims description 3
- 241000752217 Zobellella denitrificans Species 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000002028 Biomass Substances 0.000 claims 1
- -1 mixed thallus Chemical compound 0.000 claims 1
- 238000005067 remediation Methods 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 8
- 239000010865 sewage Substances 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000003344 environmental pollutant Substances 0.000 description 6
- 239000000835 fiber Substances 0.000 description 6
- 231100000719 pollutant Toxicity 0.000 description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000002504 physiological saline solution Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 235000010344 sodium nitrate Nutrition 0.000 description 3
- 239000004317 sodium nitrate Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000005696 Diammonium phosphate Substances 0.000 description 2
- 239000005909 Kieselgur Substances 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 2
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 2
- 235000019838 diammonium phosphate Nutrition 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000079938 Cellulosimicrobium funkei Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004115 Sodium Silicate Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910052911 sodium silicate Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/10—Packings; Fillings; Grids
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a preparation method of a biological filler for treating water pollution. The method comprises the following steps: (1) preparing mixed thalli; the mixed thallus consists of thallus of Microbacterium fenflurans DSM 16025, thallus of Tobexate denitrificans DSM 19707 and thallus of Oleericoccumamyloliguefacium 99B CGMCC No. 20523; (2) mixing the mixed thallus with diatomite to obtain thallus powder; (3) putting the gravel into a water glass solution for soaking; and then mixing the soaked gravel and the bacterial powder to obtain the biological filler. Experiments prove that the COD removal rate is obviously increased when the biological filler prepared by the invention is used for treating artificial sewage. The biological filler prepared by the invention can treat water pollution and has important application value.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a preparation method of a biological filler for treating water pollution.
Background
The residual culture medium of the fermentation production bacterial liquid contains a large amount of nitrogen, carbon and phosphorus pollutants, and if the bacterial liquid is directly used for water environment treatment, the bacterial liquid is equivalent to additionally introducing exogenous pollutants, so that the residual culture medium needs to be removed and then used.
Fillers and/or solid microbial agents are often needed in water environment treatment facilities such as artificial wetlands or ecological floating islands. The conventional solid microbial inoculum not only remains a large amount of pollutants of a culture medium, but also is easy to rapidly run off in a water environment. Therefore, the preparation of a biological filler which can be used for wetlands or ecological floating islands is urgently needed.
Disclosure of Invention
The invention aims to prepare a biological filler for treating water pollution.
The invention firstly protects a preparation method of a biological filler for treating water pollution, which comprises the following steps:
(1) preparing mixed thalli; the mixed thallus comprises thallus of Microbacterium febrifuga (Cellulosimicrobuenkei) DSM 16025, thallus of Zobellella denitrificans (Zobellella adenitiricas) DSM 19707, and thallus of Oleericoccumyloliquefascium 99B CGMCC No. 20523;
(2) after the step (1) is finished, mixing the mixed thallus solution with diatomite or kaolin to obtain thallus powder;
(3) soaking the filler in water glass; then mixing the soaked filler with the bacterial powder obtained in the step (2) to obtain a biological filler;
the filler is gravel, zeolite or ceramsite.
In the step (1), the ratio of the thalli of the filamentous Microbacterium cellulophilus (Cellulosimicrobuekei) DSM 16025, the thalli of the denitrifying Tolbecco (Zobellella adenitiricans) DSM 19707 and the thalli of the Oleericoccumyloliquefascium 99B CGMCC No.20523 in the mixed thalli can be 1 (0.8-1.2): (0.8-1.2) (such as 1 (0.8-1.0): 0.8-1.0), 1 (1.0-1.2): 1.0-1.2), 1:0.8:0.8, 1:1.2:1.2 and 1:1: 1: 1).
In the step (2), the mixed thallus solution can be obtained by resuspending the mixed thallus for normal saline.
In the step (2), the ratio of the physiological saline to the mixed thallus in the mixed thallus solution can be 4-6 ml: 50X 109cfu (e.g. 4-5 ml: 50X 10)9cfu、5-6ml：50×109cfu、4ml：50×109cfu、5ml：50× 109cfu or 6 ml: 50X 109cfu)。
In the step (2), the ratio of the mixed bacteria in the bacteria powder to the diatomite or the kaolin can be 50 multiplied by 109cfu: 20-30g (e.g. 50X 10)9cfu：20-25g、50×109cfu：25-30g、50×109cfu：20g、50×109cfu：25g、50×109cfu：30g)。
In the biological filler prepared by any one of the methods, the proportion of the normal saline, the mixed thallus, the diatomite or the kaolin and the filler can be 4-6 ml: 50X 109cfu: 20-30 g: 120-130g (e.g. 4 ml: 50X 10)9cfu：20g： 120g、6ml：50×109cfu: 30g of: 130g or 5 ml: 50X 109cfu：25g：125g)。
The biological filler prepared by adopting any one of the methods also belongs to the protection scope of the invention.
The application of the biological filler prepared by any one of the methods in treating water pollution also belongs to the protection scope of the invention.
In the application, the biological filler prepared by any one of the methods is added into polluted water, and then the polluted water is treated at normal temperature, so that the water pollution can be treated.
The application of any one of the mixed thalli in treating water pollution also belongs to the protection scope of the invention.
The application of any mixed thallus in the preparation of the biological filler for treating water pollution also belongs to the protection scope of the invention.
In the application, any one of the mixed bacteria is added into polluted water, and then the polluted water is treated at normal temperature, so that the water pollution can be treated. The form of adding any one of the mixed thalli can be adding any one of the biological fillers.
The artificial wetland, the ecological floating island and other water environment treatment facilities often need to use the filler. Therefore, the biological filler prepared by adopting any one of the methods can be used for wetlands or ecological floating islands.
Any of the above-described mixed cells also fall within the scope of the present invention.
Experiments prove that the COD removal rate is obviously reduced after the biological filler prepared by the invention is added into sewage and stands for 7 days at room temperature. Therefore, the biological filler prepared by the invention can treat water pollution and is used for wetlands or ecological floating islands. The invention has important application value.
Deposit description
Latin name: oleericoccum amyloliquefaciens
The strain number is as follows: 99B
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Address: xilu No. 1 Hospital No. 3 of Beijing market facing Yang district
The preservation date is as follows: year 2020, 8 and 11
Registration number of the preservation center: CGMCC No.20523
Drawings
FIG. 1 shows the result of step two in the example (from left to right, in this order, the bacterial powder B, the gravel and the biological filler B).
Detailed Description
The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The examples provided below serve as a guide for further modifications by a person skilled in the art and do not constitute a limitation of the invention in any way.
The experimental procedures in the following examples, unless otherwise indicated, are conventional and are carried out according to the techniques or conditions described in the literature in the field or according to the instructions of the products. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
In the following examples, Microcellularis finnii (Cellulosimicrobiumfunkei) DSM 16025 (hereinafter abbreviated to Microcellularis finnii) and Tolbecco denitrificans (Zobellagadenificans) DSM 19707 (hereinafter abbreviated to Tolbecco denitrificans) were both products of the German collection of microorganisms.
In the following examples, Oleericocculomiquefacium 99B CGMCC No.20523 (hereinafter referred to as "strain 99B") is disclosed in Chinese patent application CN 112226388B.
In the following examples, the fermentation medium had a solute concentration of glucose 20g/L, sodium chloride 5g/L, magnesium sulfate 0.2g/L, ammonium sulfate 1g/L, sodium nitrate 2g/L, dipotassium hydrogen phosphate 10g/L, potassium dihydrogen phosphate 5g/L and yeast extract 2g/L, and the solvent was water and had a natural pH.
Example, preparation of biological Filler and application of biological Filler in treating Water pollution
Firstly, preparation of biological filler A
1. Inoculating fen-type fiber microbe into the fermentation culture medium, and conventionally culturing to obtain bacterial liquid with concentration of 109cfu/ml of the bacterial liquid of the Fenx type fiber Microbacterium.
2. Inoculating denitrifying Zubel bacterium into a fermentation medium, and performing conventional culture to obtain a bacterium liquid with the concentration of 109cfu/ml of bacterial liquid of denitrifying Zoebel.
3. Mixing 1 volume part of the fen-type fiber microbe liquid and 1 volume part of the bacteria liquid of the denitrifying Zubel City to obtain a mixed bacteria liquid A.
4. Taking 50ml of mixed bacterium liquid A, centrifuging at 5000rpm for 5min, and collecting thalli; then, the mycelia were resuspended in 5ml of physiological saline, and 25g of diatomaceous earth was added thereto and mixed to obtain a powder A.
5. Placing 125g of gravel (the diameter is 3-5mm) into water glass (namely liquid sodium silicate, specifically a product of industrial and trade company Limited in Olympic (Beijing)) to be soaked for 5min, then fully mixing the soaked gravel and the bacterial powder A (aiming at enabling the bacterial powder to be adhered to the surface of the gravel), and airing to obtain the biological filler A.
Secondly, preparation of biological filler B
1. Inoculating fen-type fiber microbe into the fermentation culture medium, and conventionally culturing to obtain bacterial liquid with concentration of 109cfu/ml of the bacterial liquid of the Fenx type fiber Microbacterium.
2. Inoculating denitrifying Zubel bacterium into a fermentation medium, and performing conventional culture to obtain a bacterium liquid with the concentration of 109cfu/ml of bacterial liquid of denitrifying Zoebel.
3. Inoculating into fermentation mediumStrain 99B, conventionally culturing to obtain bacterial liquid with concentration of 109cfu/ml of strain 99B.
4. And mixing 1 volume part of the fen-type fiber microbe bacterial liquid, 1 volume part of the denitrification Zoebel bacterium bacterial liquid and 1 volume part of the strain 99B bacterial liquid to obtain a mixed bacterial liquid B.
5. Taking 50ml of mixed bacterium liquid B, centrifuging at 5000rpm for 5min, and collecting thalli; then, the mycelia were resuspended in 5ml of physiological saline, and 25g of diatomaceous earth was added thereto and mixed to obtain a powder B.
6. 125g of gravel (with the diameter of 3-5mm) is placed in a water glass solution to be soaked for 5min, then the soaked gravel and the bacterial powder B are fully mixed (in order to enable the bacterial powder to be adhered to the surface of the gravel) and dried to obtain the biological filler B.
Partial results are shown in FIG. 1.
Adding 25g of diatomite into 5ml of normal saline, and mixing to obtain powder; 125g of gravel (3-5 mm in diameter) was placed in a water glass solution and soaked for 5min, after which the soaked gravel and powder were mixed well (in order to make the powder stick to the gravel surface) and dried to obtain a control filler.
Application of biological filler in treating water pollution
Artificial sewage: adding glucose, sodium nitrate and diammonium phosphate into tap water to obtain artificial sewage; in the artificial sewage, the concentration of glucose is 0.1g/L, the concentration of sodium nitrate is 0.05g/L, and the concentration of diammonium phosphate is 0.05 g/L.
The biological filler to be detected is biological filler A, biological filler B or contrast filler.
Taking sterile artificial sewage, adding a biological filler to be detected (5 g of the biological filler to be detected is added into each 100ml of the sterile artificial sewage), and standing at room temperature for 7 d; then, the Chemical Oxygen Demand (COD) is detected by a rapid digestion spectrophotometry for measuring the Chemical Oxygen Demand of the water quality HJ/T399-.
The results are shown in Table 1. The results show that the removal rate of COD of the biological filler B is obviously increased compared with that of the biological filler A. Therefore, the biological filler B can treat water pollution.
TABLE 1
| COD removal rate | |
| Control filler | 7.2％ |
| Biological filler A | 25.4％ |
| Biological filler B | 59.4％ |
To further optimize the preparation process of the biological filler B, the inventors carried out a number of experimental investigations, including but not limited to:
(1) the liquid used for resuspending the thallus
Since the biological filler B is finally used for removing pollutants, and additional nitrogen and phosphorus pollution can not be introduced, the inventor conducts a large amount of experiments, and the thallus is optimally resuspended by normal saline (namely the COD removal rate is highest).
(2) Mixing the re-suspension with other substances to prepare fungus powder
Since the biological filler B is finally used for removing pollutants, and additional nitrogen and phosphorus pollution can not be introduced, the inventor finds that the bacterial powder prepared by mixing the heavy suspension and the diatomite or the kaolin is the best (namely the COD removal rate is the highest) through a large number of experiments.
(3) Optimum component for preparing biological filler B
Through a large number of experiments, the inventor finds that the gravel, the zeolite or the ceramsite and the bacterial powder are fully mixed and dried to obtain the biological filler B with the best quality (namely the highest COD removal rate).
The agent for treating the gravel, the zeolite or the ceramsite is required to be nontoxic and free of additional pollutants, and the inventor conducts a large number of experiments to ensure that the treatment effect is optimal (namely the COD removal rate is highest) by using water glass.
(4) Optimum proportion of the components
Through a large number of experiments, the inventor finds that when the biological filler B is prepared, the proportion of the physiological saline, the mixed thallus, the diatomite or the kaolin and the filler is 4-6 ml: 50X 109cfu: 20-30 g: 120-130g, the optimal proportion is 5 ml: 50X 109cfu: 25g of: 125 g; the ratio is such that the bacterial powder adheres as completely as possible, and beyond this ratio it is either loose or slushy. The ratio of the thallus of the Micromyces fibraus (Cellulosimicrobubunkei) DSM 16025 to the thallus of the Zobellella denitrificans (Zobellelladienitificans) DSM 19707 to the thallus of the Ocericoccumamycolliquefascium 99B CGMCC No.20523 in the mixed thallus is 1 (0.8-1.2) to (0.8-1.2), and the optimal ratio is 1:1: 1. The COD removal rate is highest by mixing according to the optimal proportion.
The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.
Claims (10)
1. A preparation method of biological filler for treating water pollution comprises the following steps:
(1) preparing mixed thalli; the mixed thallus comprises thallus of Microbacterium febrifuga (Cellulosimicrobuenkei) DSM 16025, thallus of Zobellella denitrificans (Zobellella adenitiricas) DSM 19707, and thallus of Oleericoccumyloliquefascium 99B CGMCC No. 20523;
(2) after the step (1) is finished, mixing the mixed thallus solution with diatomite or kaolin to obtain thallus powder;
(3) soaking the filler in water glass; then mixing the soaked filler with the bacterial powder obtained in the step (2) to obtain a biological filler;
the filler is gravel, zeolite or ceramsite.
2. The method of claim 1, wherein: in the step (1), the ratio of the thalli of the filamentous Microbacterium cellulophilus (Cellulosimicrobuekei) DSM 16025, the thalli of the denitrifying Tolbecco (Zobellladenithicans) DSM 19707 and the thalli of the Oleericoccumammalia myloliquefascium 99B CGMCC No.20523 in the mixed thalli is 1 (0.8-1.2) to (0.8-1.2).
3. The method of claim 1, wherein: and (3) in the step (2), the mixed thallus solution is prepared by resuspending the mixed thallus in normal saline.
4. The method of claim 1, wherein: in the step (2), the ratio of the mixed bacteria in the bacteria powder to the diatomite or the kaolin is 50 multiplied by 109cfu：20-30g。
5. The method of claim 1, wherein: in the biological filler, the proportion of normal saline, mixed thallus, diatomite or kaolin and the filler is 4-6 ml: 50X 109cfu：20-30g：120-130g。
6. A biological filler produced by the production method according to any one of claims 1 to 5.
7. Use of the biological filler prepared according to claim 6 for treating water pollution.
8. The use of the mixed bacterial cells of claim 1 or 2 for treating water pollution.
9. Use of the mixed biomass according to claim 1 or 2 in the preparation of a biofilm carrier for the remediation of water pollution.
10. The mixed bacterial cell according to claim 1 or 2.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR9815130A (en) * | 1997-12-01 | 2000-10-10 | Eisu Innovative Ges Fuer Techn | Biosorbents and process for their production |
| US20160186111A1 (en) * | 2014-11-06 | 2016-06-30 | Thomas Benton Young, III | Municipal waste water treatment plant/process with by product and drinking water recycle |
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