CN112973822B - Protein separation and purification device and purification method - Google Patents
Protein separation and purification device and purification method Download PDFInfo
- Publication number
- CN112973822B CN112973822B CN202110298059.3A CN202110298059A CN112973822B CN 112973822 B CN112973822 B CN 112973822B CN 202110298059 A CN202110298059 A CN 202110298059A CN 112973822 B CN112973822 B CN 112973822B
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- Prior art keywords
- annular
- annular shell
- liquid outlet
- outlet pipe
- shell
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 38
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 38
- 238000000746 purification Methods 0.000 title claims abstract description 19
- 238000000926 separation method Methods 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims description 11
- 239000007788 liquid Substances 0.000 claims abstract description 55
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052742 iron Inorganic materials 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 238000004587 chromatography analysis Methods 0.000 abstract description 8
- 238000007789 sealing Methods 0.000 abstract 2
- 238000005194 fractionation Methods 0.000 description 2
- 238000005377 adsorption chromatography Methods 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
- B01L3/50825—Closing or opening means, corks, bungs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a protein separation and purification device, which comprises an annular shell and a chromatography column on an iron support, wherein a fixing plate with an annular structure is arranged in an opening at the upper end of the annular shell, more than one liquid inlet hopper is arranged on the fixing plate, more than one annular groove is arranged on the top surface of an annular seat, the lower end of the annular shell is inserted into the corresponding annular groove, slots are arranged at positions of the annular seat opposite to the liquid inlet hoppers, test tubes are arranged in the slots, the upper ends of the test tubes are sleeved outside the liquid inlet hoppers, a rubber layer is arranged on the surface of the fixing plate, a cover plate with an annular structure is arranged at the top of the annular shell, through holes are arranged on the cover plate, a liquid outlet pipe is arranged at the lower end of the chromatography column, and the lower end of the liquid outlet pipe is inserted into the annular shell along the through holes. The liquid outlet pipe sealing device is simple in structure, the liquid outlet hole below the liquid outlet pipe can be sealed in the annular shell, contact with outside air is avoided, sealing and opening of the liquid outlet pipe can be automatically completed when a test tube is replaced, and convenience and efficiency are greatly improved.
Description
Technical Field
The invention relates to the technical field of protein purification, in particular to a protein separation and purification device and a protein separation and purification method.
Background
A typical eukaryotic cell may contain thousands of different proteins, and in order to study a protein, the protein must first be purified from other protein and non-protein molecules. Therefore, the separation and purification of proteins is widely used in research and application of biochemistry and the like, is an important operation technology, mainly utilizes the differences of the sizes, shapes, charges, hydrophobicity, solubility, biological activity and the like of various proteins to purify target proteins from other proteins, and a general procedure can be divided into three steps of pretreatment, coarse fractionation and fine fractionation. Wherein, the fine-grading separation generally adopts chromatography methods, including gel filtration chromatography, ion exchange chromatography, adsorption chromatography, affinity chromatography and the like. Different particle fillers are loaded in the chromatographic column according to different purposes, and after a sample passes through the chromatographic column, the separated and purified proteins flow out in sequence and are respectively collected in different test tubes.
At present, the protein of outflow exposes earlier in the air in the external world, flows into the test tube that corresponds again, causes the influence to protein easily, and changes a test tube at every turn, and the drain pipe on the chromatography column need be closed more, and after the change is accomplished, opening the drain pipe, the operation is comparatively troublesome, and efficiency is influenced, causes protein to trickle to the external world easily, causes pollution and waste.
Disclosure of Invention
The technical problem to be solved by the present invention is to provide a protein separation and purification device and a protein separation and purification method, so as to solve the problems proposed in the background art.
The invention is realized by the following technical scheme: a protein separation and purification device comprises an annular shell and a chromatography column on an iron support, wherein a fixed plate with an annular structure is arranged in an opening at the upper end of the annular shell, more than one liquid inlet hopper is arranged on the fixed plate, an annular seat is arranged at the lower end of the annular shell, more than one annular groove is arranged on the top surface of the annular seat, the lower end of the annular shell is inserted into the corresponding annular groove, slots are respectively arranged at positions, right opposite to the liquid inlet hoppers, of the annular seat, test tubes are respectively arranged in the slots, the upper ends of the test tubes are sleeved outside the liquid inlet hoppers, a rubber layer is arranged on the surface of the fixed plate, rectangular openings are respectively arranged at positions, right opposite to the liquid inlet hoppers, a cover plate with an annular structure is arranged at the top of the annular shell, through holes are arranged on the cover plate, rubber rings are arranged on the inner wall surfaces of the through holes, a liquid outlet pipe is arranged at the lower end of the chromatography column, the lower end of the liquid outlet pipe is inserted into the annular shell along the through holes, the bottom surface of the liquid outlet pipe and the surface of the rubber layer, the inner ring surface of the rubber ring is propped against the outer wall surface of the liquid outlet pipe.
As the preferred technical scheme, the lower end of the annular shell is provided with a bottom plate, the surface of the bottom plate is upwards protruded to form an annular structure fixing part, the surface of the fixing part is provided with a first positioning groove of an annular structure, the bottom surface of the annular seat is outwards protruded to form a first positioning part of the annular structure, and the first positioning part is movably arranged in the first positioning groove.
As the preferred technical scheme, be equipped with the second constant head tank of loop configuration on the terminal surface of annular shell upper end, the bottom surface of apron all outwards protrudes the second location portion that forms a loop configuration just to second constant head tank department, and second location portion movable mounting is in the second constant head tank.
As the preferred technical scheme, more than one toggle strip is arranged on the outer wall surface of the annular shell.
As a preferred technical scheme, the cover plate, the fixing plate and the annular shell are all made of transparent plastic materials.
Preferably, the rubber layer is made of transparent silica gel material.
As the preferred technical scheme, the outer wall surface of the annular shell is outwards protruded to form a clamping strip of an annular structure, the inner wall surface of the annular groove is inwards sunken to form a clamping groove of the annular structure, and one end of the clamping strip is movably arranged in the clamping groove.
The invention also provides a protein separation and purification method which is realized by adopting the protein separation and purification device.
The invention has the beneficial effects that: the liquid outlet pipe is simple in structure, after the liquid outlet pipe is inserted into the through hole, the liquid outlet hole below the liquid outlet pipe can be sealed in the annular shell, so that the contact with outside air is avoided, protein can directly enter the test tube along the liquid inlet hopper, the test tube can be driven after the annular shell is rotated, the test tube is effectively replaced, once the liquid outlet pipe is moved away from the upper side of the liquid inlet hopper, the end face of the lower end of the liquid outlet pipe can be abutted against the rubber layer to directly seal the liquid outlet pipe, when other test tubes are not aligned, the liquid outlet pipe is always sealed, the outflow of protein is avoided, after the liquid outlet pipe is moved to the next test tube, the blocking of the rubber layer is lost, so that the protein in the liquid outlet pipe smoothly flows out, the replacement of the test tube can be directly completed in the process, the liquid outlet pipe can be closed and opened, and the convenience and the efficiency are greatly improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the installation structure of the liquid inlet funnel and the fixing plate according to the present invention;
figure 3 is a top view of the annular seat of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
In the description of the present invention, it should be noted that unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can, for example, be fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1, 2 and 3, the protein separation and purification device of the present invention comprises an annular shell 5 and a chromatography column 1 on an iron support, wherein an opening at the upper end of the annular shell 5 is provided with a fixing plate 6 with an annular structure, the fixing plate 6 is provided with more than one liquid inlet hopper 7, the lower end of the annular shell 5 is provided with an annular seat 12, the top surface of the annular seat 12 is provided with more than one annular groove 17, the lower end of the annular shell 5 is inserted into the corresponding annular groove 17, the annular seat 12 is provided with slots 16 opposite to the liquid inlet hoppers 7, the slots 16 are provided with test tubes 9, the upper ends of the test tubes 9 are sleeved outside the liquid inlet hoppers 7, the surface of the fixing plate 6 is provided with a rubber layer, the rubber layer is provided with a rectangular opening opposite to the liquid inlet hoppers, the top of the annular shell 5 is provided with a cover plate 4 with an annular structure, the cover plate 4 is provided with a through hole, the inner wall of the through hole is provided with a rubber ring 3, liquid outlet pipe 2 is installed to the lower extreme of chromatography post 1, and in liquid outlet pipe 2 lower extreme inserted the annular shell along the through-hole, the bottom surface of liquid outlet pipe 2 and the surface on rubber layer, the inner circle face of rubber circle 3 supported with the outer wall face of liquid outlet pipe 2.
In this embodiment, a bottom plate 14 is disposed at the lower end of the annular housing 5, a ring-shaped fixing portion 13 is formed by upward protruding the surface of the bottom plate 14, a first positioning groove of a ring-shaped structure is disposed on the surface of the fixing portion 13, a first positioning portion 15 of a ring-shaped structure is formed by outward protruding the bottom surface of the annular seat 12, and the first positioning portion 15 is movably mounted in the first positioning groove, so that the annular housing rotates along the first positioning groove.
In this embodiment, be equipped with the second constant head tank of loop configuration on the terminal surface of annular shell 5 upper end, the bottom surface of apron 4 just all outwards protrudes the second location portion that forms an loop configuration to second constant head tank department, and second location portion movable mounting is in the second constant head tank, when the annular shell rotates, can fix a position the apron through the drain pipe, and the apron can be all the time with the opening seal of annular shell upper end, has avoided the entering of outside air.
In this embodiment, more than one toggle bar 8 is installed on the outer wall surface of the annular housing 5, which facilitates the rotation of the annular housing in the annular seat.
In this embodiment, the cover plate 4, the fixing plate 6 and the annular housing 5 are made of a transparent plastic material; the rubber layer is made of transparent silica gel material, so that the worker can see the inner condition through the rubber layer and the annular shell.
In this embodiment, the outer wall surface of the annular housing 5 protrudes outward to form the clamping strip 11 with an annular structure, the inner wall surface of the annular groove 17 protrudes inward to form the clamping groove with an annular structure, and one end of the clamping strip 11 is movably installed in the clamping groove, so that the clamping strip can rotate along the clamping groove.
The invention also provides a protein separation and purification method, which is realized by adopting the device and specifically comprises the following steps: the test tube is inserted into the slot, the annular shell is inserted into the annular slot and is clamped and fixed through the clamping strip and the clamping slot, the rotation of the annular shell is not influenced because the clamping strip and the clamping slot are arranged in an annular structure, after the annular shell is fixed, the liquid outlet tube at the lower end of the chromatographic column is inserted into the through hole and is right opposite to the liquid inlet hopper, a gap between the chromatographic column and the through hole is sealed through the rubber ring, so that the contact with the outside air is avoided, protein can directly enter the test tube along the liquid inlet hopper, the annular shell can be rotated after one test tube is fully stored, the annular shell is rotated along the clamping slot, the test tube is driven by the rotation of the annular shell, the test tube is effectively replaced, once the liquid outlet tube is moved away from the upper part of the liquid inlet hopper, the end face at the lower end of the liquid outlet tube can abut against the rubber layer, the liquid outlet tube is directly sealed, when other test tubes are not aligned, the liquid outlet tube is always sealed, and the outflow of the protein is avoided, and after the drain pipe moved to next test tube, can lose stopping of rubber layer, make the smooth outflow of inside protein, the replacement of the completion test tube that this process can be direct also can carry out closing and opening of drain pipe, great increase convenience and efficiency.
The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the inventive work should be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope defined by the claims.
Claims (6)
1. A protein separation and purification device is characterized in that: including annular shell (5) and chromatographic column (1) on the iron stand platform, install fixed plate (6) of loop configuration in the opening of annular shell (5) upper end, install more than one on fixed plate (6) and advance liquid fill (7), annular shell (5) lower extreme is equipped with annular seat (12), be equipped with more than one ring channel (17) on the top surface of annular seat (12), annular shell (5) lower extreme inserts in corresponding ring channel (17), annular seat (12) just all are equipped with slot (16) to advancing liquid fill (7) department, all be equipped with test tube (9) in slot (16), the liquid fill (7) outside is located to test tube (9) upper end cover, install the rubber layer on the surface of fixed plate (6), the rubber layer just all is equipped with the rectangle mouth to advancing liquid fill department, the top of annular shell (5) is equipped with apron (4) of loop configuration, be equipped with the through-hole on apron (4), the inner wall surface of the through hole is provided with a rubber ring (3), the lower end of the chromatographic column (1) is provided with a liquid outlet pipe (2), the lower end of the liquid outlet pipe (2) is inserted into the annular shell along the through hole, the bottom surface of the liquid outlet pipe (2) is abutted to the surface of the rubber layer, and the inner ring surface of the rubber ring (3) is abutted to the outer wall surface of the liquid outlet pipe (2); the lower end of the annular shell (5) is provided with a bottom plate (14), the surface of the bottom plate (14) protrudes upwards to form an annular structure fixing part (13), the surface of the fixing part (13) is provided with a first positioning groove of an annular structure, the bottom surface of the annular seat (12) protrudes outwards to form a first positioning part (15) of the annular structure, and the first positioning part (15) is movably arranged in the first positioning groove; the end face of the upper end of the annular shell (5) is provided with a second positioning groove of an annular structure, the bottom surface of the cover plate (4) is opposite to the second positioning groove and protrudes outwards to form a second positioning part of the annular structure, and the second positioning part is movably installed in the second positioning groove.
2. The protein separating and purifying apparatus according to claim 1, wherein: more than one toggle strip (8) is arranged on the outer wall surface of the annular shell (5).
3. The protein separation and purification apparatus according to claim 1, wherein: the cover plate (4), the fixing plate (6) and the annular shell (5) are all made of transparent plastic materials.
4. The protein separation and purification apparatus according to claim 1, wherein: the rubber layer is made of transparent silica gel material.
5. The protein separating and purifying apparatus according to claim 1, wherein: the outer wall surface of the annular shell (5) is outwards protruded to form a clamping strip (11) with an annular structure, the inner wall surface of the annular groove (17) is inwards sunken to form a clamping groove with an annular structure, and one end of the clamping strip (11) is movably arranged in the clamping groove.
6. A method for separating and purifying protein is characterized in that: the device of any one of claims 1 to 5 is used to achieve separation and purification of proteins.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110298059.3A CN112973822B (en) | 2021-03-19 | 2021-03-19 | Protein separation and purification device and purification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110298059.3A CN112973822B (en) | 2021-03-19 | 2021-03-19 | Protein separation and purification device and purification method |
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| Publication Number | Publication Date |
|---|---|
| CN112973822A CN112973822A (en) | 2021-06-18 |
| CN112973822B true CN112973822B (en) | 2022-09-16 |
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| CN202110298059.3A Expired - Fee Related CN112973822B (en) | 2021-03-19 | 2021-03-19 | Protein separation and purification device and purification method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001124790A (en) * | 1999-10-28 | 2001-05-11 | Tokyo Rika Kikai Kk | Powder and particle sample determining and distributing device |
| CN210356116U (en) * | 2019-04-26 | 2020-04-21 | 贵州师范学院 | Chemical experiment extraction device |
| CN212396780U (en) * | 2020-04-03 | 2021-01-26 | 佛山市天光源环保检测服务有限公司 | Test tube water bath subassembly is used in inspection |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10167202A (en) * | 1996-12-12 | 1998-06-23 | Saburo Suzuki | Charging method and device of liquid into thermoplastic resin container at high-temperature |
| JP3273916B2 (en) * | 1998-03-30 | 2002-04-15 | 日本たばこ産業株式会社 | Analysis pretreatment system |
| EP1307289A4 (en) * | 2000-08-02 | 2005-07-27 | Nl Technologies Ltd | System for multiple sterile sample collection and isolation |
| JP4646809B2 (en) * | 2003-12-26 | 2011-03-09 | パナソニック株式会社 | Biological sample discrimination device, biological sample discrimination method, and biological sample discrimination plate |
| EP2067529B1 (en) * | 2007-12-06 | 2012-11-14 | Mettler-Toledo AG | Siphoning device for laboratory containers |
| DE102010063144A1 (en) * | 2010-12-15 | 2012-06-21 | Krones Aktiengesellschaft | Fluid rotary distributor |
| CN108072547A (en) * | 2018-01-21 | 2018-05-25 | 新昌县七星街道新伟机械厂 | A kind of acid rain automatic sampler of environmental test |
| CN109060437A (en) * | 2018-08-14 | 2018-12-21 | 南京唯诺尔科技项目管理有限公司 | A kind of sampler for biotechnology |
| CN210171483U (en) * | 2019-06-28 | 2020-03-24 | 贵阳学院 | Novel liquid distributor applied in chemical experiment process |
| CN211042840U (en) * | 2019-12-04 | 2020-07-17 | 贝丽萍 | Oil pumping detection equipment for oil production field management |
| CN212300961U (en) * | 2020-05-21 | 2021-01-05 | 甘肃省化工研究院有限责任公司 | Chemical industry raw material sampling extraction element for chemical production |
| CN212722165U (en) * | 2020-07-06 | 2021-03-16 | 四川和鉴检测技术有限公司 | Water sample collection device |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001124790A (en) * | 1999-10-28 | 2001-05-11 | Tokyo Rika Kikai Kk | Powder and particle sample determining and distributing device |
| CN210356116U (en) * | 2019-04-26 | 2020-04-21 | 贵州师范学院 | Chemical experiment extraction device |
| CN212396780U (en) * | 2020-04-03 | 2021-01-26 | 佛山市天光源环保检测服务有限公司 | Test tube water bath subassembly is used in inspection |
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| CN112973822A (en) | 2021-06-18 |
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