CN112958015B - System and method for recombining shell structure by bubble-assisted sound wave - Google Patents
System and method for recombining shell structure by bubble-assisted sound wave Download PDFInfo
- Publication number
- CN112958015B CN112958015B CN202110190011.0A CN202110190011A CN112958015B CN 112958015 B CN112958015 B CN 112958015B CN 202110190011 A CN202110190011 A CN 202110190011A CN 112958015 B CN112958015 B CN 112958015B
- Authority
- CN
- China
- Prior art keywords
- bubble
- frequency
- inlet
- hydrogel
- layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
- B01J13/04—Making microcapsules or microballoons by physical processes, e.g. drying, spraying
- B01J13/046—Making microcapsules or microballoons by physical processes, e.g. drying, spraying combined with gelification or coagulation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00788—Three-dimensional assemblies, i.e. the reactor comprising a form other than a stack of plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00801—Means to assemble
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00851—Additional features
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00925—Irradiation
- B01J2219/00932—Sonic or ultrasonic vibrations
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The invention discloses a system for recombining a shell structure by bubble-assisted sound waves, which comprises: the bubble generation module comprises a microfluidic chip and a first inlet; the micro-fluidic chip is provided with a micro-fluidic channel, and the first inlet is connected with the micro-fluidic channel; the first inlet is used for conveying hydrogel with a certain concentration to the microfluidic channel to form a bubble core; the bubble size control module comprises a high-frequency piezoelectric sheet and is used for generating high-frequency sound waves to grow bubbles; the cell manipulation module comprises a second inlet and a low-frequency piezoelectric sheet, the second inlet is used for conveying the pretreated cells and the hydrogel to the microfluidic channel, and the low-frequency piezoelectric sheet is used for generating low-frequency sound waves to enable the cells in the sample to form a spherical surface around the bubble agglomeration; the signal generator is used for inputting corresponding signals to the high-frequency piezoelectric sheet and the low-frequency piezoelectric sheet; and the curing module is used for curing the hydrogel in the sample after the cell aggregation is finished. The system has simple structure, and can accurately control the cell recombination shell structure through sound waves.
Description
Technical Field
The invention relates to the field of micro-nano material control, in particular to a system and a method for recombining a shell structure by using bubbles to assist sound waves.
Background
The micro-nano material control is a tool which is urgently needed in various fields, in particular to the field of life science. The contact type tool comes to the micron level, the precision and the portability are greatly reduced, and the tool cannot be developed. Non-contact is an ideal tool and includes optical, magnetic, electrical, acoustic, etc. that can produce non-contact mechanical effects. But the sound force is most concerned in the operation and control of the living body, and has the best application prospect.
Microfluidic technology is a new field across disciplines, and has developed rapidly in recent years. The micro-fluidic chip has the advantages of small size, low cost, high integration and the like, and has the advantages of good control and detection of fluid and cells in a micron scale. In recent years, contact tool bubbles have gained wide attention in microfluidics by virtue of their simple, green-friendly characteristics. In particular, the combination of bubbles with the acoustic field produces many synergistic effects that can be used for more complex control of particles than the acoustic field alone. Such as for drug delivery, cell sorting and patterning. However, there are many limitations to the efficient use of bubbles in microfluidics, such as the controllability of the size and location of the bubbles.
Disclosure of Invention
The invention aims to provide a system for recombining a shell structure by bubble-assisted sound waves, which enriches the spatial form of cells formed by operation arrangement and provides a new arrangement method.
In order to solve the technical problem, the invention provides a technical scheme that: the system comprises:
the bubble generation module comprises a microfluidic chip and a first inlet; the micro-fluidic chip is provided with a micro-fluidic channel, and the first inlet is connected with the micro-fluidic channel; the first inlet is used for conveying hydrogel with a certain concentration to the microfluidic channel to form a bubble core; the micro-fluidic channel consists of a first layer of cavity array and a second layer of square cavity, the first layer of cavity array is communicated with the second layer of square cavity, and the first layer of cavity array is positioned above the second layer of square cavity;
the bubble size control module comprises a high-frequency piezoelectric sheet and is used for generating high-frequency sound waves to enable bubbles in the microfluidic channel to grow up and controlling the sizes of the bubbles by adjusting the action time of the high-frequency sound waves;
the cell manipulation module comprises a second inlet and a low-frequency piezoelectric sheet, the second inlet is used for conveying the pretreated cells and the hydrogel to the microfluidic channel, the low-frequency piezoelectric sheet is used for generating low-frequency sound waves, and the cells in the sample are agglomerated around the bubbles to form a spherical surface by utilizing the acoustic radiation force reflected by the surfaces of the bubbles and combining the drag force of the fluid;
the signal generator is used for inputting corresponding signals to the high-frequency piezoelectric sheet and the low-frequency piezoelectric sheet;
and the curing module is used for curing the hydrogel in the sample after the cell aggregation is finished to form a shell layer.
According to the scheme, the height of the cavity array of the first layer is 50 micrometers, the cavity array of the first layer is composed of 5 multiplied by 5 cylindrical units, the radius of a cross section circle of each unit is 50 micrometers, and the distance between the units is 700 micrometers.
According to the scheme, the height of the square cavity of the second layer is 250 micrometers.
According to the scheme, the microfluidic channel is also connected with a sample outlet.
According to the scheme, one end of each of the first inlet and the second inlet is connected with the microfluidic channel, and the other end of each of the first inlet and the second inlet is connected with the microfluidic pump.
According to the scheme, the system further comprises a glass slide, and the microfluidic chip, the high-frequency piezoelectric sheet and the low-frequency piezoelectric sheet are all fixed on the glass slide.
According to the scheme, the high-frequency piezoelectric plate is connected with the power amplifier through a first positive electrode signal input end and a first negative electrode signal input end, and the low-frequency piezoelectric plate is connected with the power amplifier through a second positive electrode signal input end and a second negative electrode signal input end; the power amplifier is connected with the signal generator.
A method for recombining shells by bubble-assisted sound waves comprises the following steps:
s1, preparing 5% hydrogel, and dividing into two parts;
s2, using one part of hydrogel to pretreat the cells to obtain a sample with a certain cell concentration;
s3, conveying the other part of hydrogel to the microfluidic channel from the first inlet through the microfluidic pump, and generating bubble cores in the first layer of cavity array after the hydrogel enters the microfluidic channel;
s4, setting the frequency and power of the radio frequency signal of the signal generator to enable the high-frequency piezoelectric sheet to generate high-frequency sound waves to enlarge bubbles, and controlling the action time of the sound waves by controlling the switch of the signal generator so as to realize the control of the sizes of the bubbles;
s5, conveying the sample to the microfluidic channel from the second inlet through the microfluidic pump, setting the frequency and power of the radio frequency signal of the signal generator again to enable the low-frequency piezoelectric plate to generate low-frequency sound waves, and enabling particle cells in the sample to form a spherical surface around the bubble agglomeration by utilizing the acoustic radiation force reflected by the surface of the bubble and combining the drag force of the fluid;
and S6, after the particle cells are agglomerated, solidifying the hydrogel in the sample by utilizing a solidifying module, and finishing the manufacturing of the shell layer structure.
According to the scheme, the pretreatment specifically comprises the following steps: human ovarian granulosa cells with an adherent area of more than 85% were trypsinized, centrifuged and resuspended in 1ml of 5% hydrogel in a 4ml petri dish.
The invention has the beneficial effects that: the sample can enter the microfluidic channel to form a bubble core by arranging the bubble generation module; the bubble size control module can realize the control of the size of the bubbles by generating high-frequency sound waves and controlling the action duration of the high-frequency sound waves; the cell manipulation module can enable cells in the sample to form a spherical surface around the bubbles by generating low-frequency sound waves; the curing module can cure the hydrogel in the sample, so that the cells which are agglomerated around the bubbles are fixed to form a shell layer; according to the invention, the high-frequency sound wave is adopted to control the size of the bubbles, and then the low-frequency sound wave is adopted to control the cells, so that the damage of heat generated by the high-frequency sound wave to the cells is avoided.
Further, the microfluidic channel is provided with the first layer of cavity array and the second layer of square cavity, so that the efficiency of capturing bubble nuclei generated after the sample flows into the microfluidic channel can reach one hundred percent.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic diagram of a vertical structure according to an embodiment of the present invention;
FIG. 3 is a micrograph of bubble generation and size control according to one embodiment of the present invention;
FIG. 4 is a fluorescence micrograph of particle control according to one embodiment of the present invention;
FIG. 5 is a micrograph of different focal planes of granulosa cells after spheronization in accordance with one embodiment of the present invention;
in the figure: 1-a micro-fluidic chip, 2-a first inlet, 3-a sample outlet, 4-a second inlet, 5-a high-frequency piezoelectric sheet, 6-a low-frequency piezoelectric sheet, 7-a first positive electrode signal input end, 8-a first negative electrode signal input end, 9-a second positive electrode signal input end, 10-a second negative electrode signal input end, 11-a glass slide, 101-a first layer of cavity array and 102-a second layer of square cavity.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more apparent, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings of the embodiments of the present disclosure. It is to be understood that the described embodiments are only a few embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the disclosure without any inventive step, are within the scope of protection of the disclosure.
Referring to fig. 1, the system for bubble assisted acoustic recombination shell structure comprises:
the bubble generation module comprises a micro-fluidic chip 1 and a first inlet 2, wherein the micro-fluidic chip 1 is provided with a micro-fluidic channel, and the first inlet 2 is connected with the micro-fluidic channel; the first inlet 2 is used for conveying hydrogel with a certain concentration to the microfluidic channel to form a bubble core; in this embodiment, the microfluidic chip 1 is made of Polydimethylsiloxane (PDMS), which is an organic material, and the specific manufacturing method is as follows: drawing the shapes of a first layer cavity array 101 and a second layer square cavity 102 in the microfluidic channel by using software, respectively manufacturing mask plates according to the shapes, developing the shapes on a silicon wafer in sequence by using an ultraviolet lithography technology to obtain a silicon wafer mold of the microfluidic channel, pouring uncured PDMS on the silicon wafer mold, and baking for one hour at the temperature of 75 ℃ to solidify to obtain the microfluidic chip 1; the cells in this example are human ovarian granulosa cells, the hydrogel is methacrylated gelatin (GelMA), model number EFL-GM-60;
the bubble size control module comprises a high-frequency piezoelectric sheet 5, is used for generating high-frequency sound waves to enable bubbles to grow up, and realizes the control of the bubble size by adjusting the action time of the high-frequency sound waves; in the embodiment, the high-frequency piezoelectric sheet 5 is a 3MHz piezoelectric sheet;
the cell manipulation module comprises a second inlet 4 and a low-frequency piezoelectric sheet 6, wherein the second inlet 4 is used for conveying the pretreated cells and hydrogel to the microfluidic channel, the low-frequency piezoelectric sheet 6 is used for generating low-frequency sound waves, and the particle cells in the sample are agglomerated around the bubbles to form a spherical surface by utilizing the acoustic radiation force reflected by the surfaces of the bubbles and combining with the drag force of the fluid; in the embodiment, the low-frequency piezoelectric sheet 6 is a 131kHz piezoelectric sheet;
the signal generator is used for inputting corresponding signals to the high-frequency piezoelectric sheet 5 and the low-frequency piezoelectric sheet 6;
the curing module is used for curing the hydrogel in the sample to form a shell layer; in this embodiment, the curing module is a 405nm blue light lamp.
Further, the microfluidic channel is composed of a first layer of cavity array 101 and a second layer of square cavity 102, the first layer of cavity array 101 is communicated with the second layer of square cavity 102, and the first layer of cavity array 101 is located above the second layer of square cavity 102.
Further, the first-layer cavity array 101 has a height of 50 μm, and is composed of a 5 × 5 array of cylindrical cells, the cross-sectional circle radius of the cells is 50 μm, and the distance between the cells is 700 μm.
Further, the second layer square cavity 102 has a height of 250 μm.
Further, the microfluidic channel is also connected with a sample outlet 3.
Furthermore, one end of each of the first inlet 2 and the second inlet 4 is connected with the microfluidic channel, and the other end is connected with a microfluidic pump.
Further, the system also comprises a glass slide 11, and the microfluidic chip 1, the high-frequency piezoelectric sheet 5 and the low-frequency piezoelectric sheet 6 are all fixed on the glass slide 11.
Further, the high-frequency piezoelectric patch 5 is connected with the power amplifier through a first positive electrode signal input end 7 and a first negative electrode signal input end 8, and the low-frequency piezoelectric patch 6 is connected with the power amplifier through a second positive electrode signal input end 9 and a second negative electrode signal input end 10; the power amplifier is connected with the signal generator; the signal generator can adjust the frequency of the output radio frequency signal to match the frequency of the piezoelectric sheet, and the power amplifier can adjust the output power of the signal generator.
A method for recombining shells by bubble-assisted sound waves comprises the following steps:
s1, preparing 5% hydrogel, and dividing the hydrogel into two parts;
s2, digesting the human ovarian granulosa cells with the adherent area of more than 85% by pancreatin in a 4ml culture dish, centrifuging, and resuspending with 1ml of 5% hydrogel to obtain the cell concentration of 3X 106one/mL asPreparing a sample for later use;
s3, pushing another part of hydrogel into the microfluidic channel from the first inlet 2 at a speed of 3mL/h by using the microfluidic pump, and generating bubble nuclei in the first layer of cavity array 101 after the hydrogel enters the microfluidic channel;
s4, setting the signal generator to generate a 3MHz radio frequency signal, setting the power to 1.6w through the power amplifier, connecting the high-frequency piezoelectric sheet 5 through the first positive electrode signal input end 7 and the first negative electrode signal input end 8, generating high-frequency sound waves by the high-frequency piezoelectric sheet 5 to enlarge bubbles, and controlling the action time of the sound waves by controlling the switch of the signal generator so as to realize the control of the sizes of the bubbles; see figure 3 for bubble size variation;
s5, delivering the sample to the microfluidic channel from the second inlet 4 by the microfluidic pump, setting the signal generator to generate a radio frequency signal of 131kHz, setting the power to 1.6w by the power amplifier, and connecting the low-frequency piezoelectric plate 5 to the second positive signal input end 9 and the second negative signal input end 10, where the low-frequency piezoelectric plate 5 generates a low-frequency acoustic wave, and using the acoustic radiation force reflected by the surface of the bubble, combining the drag force of the fluid to make the particle cells in the sample agglomerate around the bubble to form a spherical surface, as shown in fig. 4;
s6, after the particle cells are agglomerated, vertically irradiating the microfluidic chip 1 by using a 403nm blue light lamp to solidify the hydrogel in the sample, and finishing the manufacturing of the shell structure, as shown in FIG. 5.
In summary, the invention provides a system for a bubble-assisted acoustic wave recombination shell structure, hydrogel is conveyed to a microfluidic channel from a first inlet through a microfluidic pump, and a bubble core is formed after the hydrogel enters the microfluidic channel; the frequency and the power of the signal generator are adjusted to transmit radio-frequency signals to the high-frequency piezoelectric sheet, and the high-frequency piezoelectric sheet generates high-frequency sound waves to grow bubbles; the pretreated cells and hydrogel are conveyed to the microfluidic channel from the second inlet through the microfluidic pump, the frequency and the power of the signal generator are adjusted again, radio-frequency signals are transmitted to the low-frequency piezoelectric sheet, the low-frequency piezoelectric sheet generates low-frequency sound waves, and the cells are agglomerated around bubbles to form a spherical surface; and (5) solidifying the hydrogel by adopting a solidifying module, and finishing the manufacturing of the shell structure of the cells.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (9)
1. A system for recombining a shell structure by bubble-assisted sound waves is characterized in that: the system comprises:
the bubble generation module comprises a microfluidic chip and a first inlet; the micro-fluidic chip is provided with a micro-fluidic channel, and the first inlet is connected with the micro-fluidic channel; the first inlet is used for conveying hydrogel with a certain concentration to the microfluidic channel to form a bubble core; the micro-fluidic channel consists of a first layer of cavity array and a second layer of square cavity, the first layer of cavity array is communicated with the second layer of square cavity, and the first layer of cavity array is positioned above the second layer of square cavity;
the bubble size control module comprises a high-frequency piezoelectric sheet and is used for generating high-frequency sound waves to enable bubbles in the microfluidic channel to grow up and controlling the sizes of the bubbles by adjusting the action time of the high-frequency sound waves;
the cell manipulation module comprises a second inlet and a low-frequency piezoelectric sheet, the second inlet is used for conveying the pretreated cells and the hydrogel to the microfluidic channel, the low-frequency piezoelectric sheet is used for generating low-frequency sound waves, and the cells in the sample are agglomerated around the bubbles to form a spherical surface by utilizing the acoustic radiation force reflected by the surfaces of the bubbles and combining the drag force of the fluid;
the signal generator is used for inputting corresponding signals to the high-frequency piezoelectric sheet and the low-frequency piezoelectric sheet;
and the curing module is used for curing the hydrogel in the sample after the cell aggregation is finished to form a shell layer.
2. The system of bubble assisted acoustic recombination shell structure of claim 1, wherein: the height of the first layer cavity array is 50 mu m, the first layer cavity array is composed of 5 multiplied by 5 array cylindrical units, the radius of a cross section circle of each unit is 50 mu m, and the distance between the units is 700 mu m.
3. The system of bubble assisted acoustic recombination shell structures of claim 1 or 2, wherein: the second layer square cavity height is 250 μm.
4. The system of bubble assisted acoustic recombination shell structure of claim 1, wherein: the microfluidic channel is also connected with a sample outlet.
5. The system of bubble assisted acoustic recombination shell structure of claim 1, wherein: one end of the first inlet and one end of the second inlet are connected with the micro-fluidic channel, and the other end of the first inlet and the second inlet are connected with a micro-flow pump.
6. The system of bubble assisted acoustic recombination shell structure of claim 1 or claim, wherein: the system also comprises a glass slide, and the microfluidic chip, the high-frequency piezoelectric sheet and the low-frequency piezoelectric sheet are all fixed on the glass slide.
7. The system of bubble assisted acoustic recombination shell structure of claim 1, wherein: the high-frequency piezoelectric plate is connected with the power amplifier through a first positive electrode signal input end and a first negative electrode signal input end, and the low-frequency piezoelectric plate is connected with the power amplifier through a second positive electrode signal input end and a second negative electrode signal input end; the power amplifier is connected with the signal generator.
8. A method for reconstructing a shell structure of cells using the system for reconstructing a shell structure of acoustic waves assisted by bubbles according to any one of claims 1 to 7, wherein: the method comprises the following steps:
s1, preparing 5% hydrogel, and dividing into two parts;
s2, using one part of hydrogel to pretreat the cells to obtain a sample with a certain cell concentration;
s3, conveying the other part of hydrogel to the microfluidic channel from the first inlet through the microfluidic pump, and generating bubble cores in the first layer of cavity array after the hydrogel enters the microfluidic channel;
s4, setting the frequency and power of the radio frequency signal of the signal generator to enable the high-frequency piezoelectric sheet to generate high-frequency sound waves to enlarge bubbles, and controlling the action time of the sound waves by controlling the switch of the signal generator so as to realize the control of the sizes of the bubbles;
s5, conveying the sample to the microfluidic channel from the second inlet through the microfluidic pump, setting the frequency and power of the radio frequency signal of the signal generator again to enable the low-frequency piezoelectric plate to generate low-frequency sound waves, and enabling particle cells in the sample to form a spherical surface around the bubble agglomeration by utilizing the acoustic radiation force reflected by the surface of the bubble and combining the drag force of the fluid;
and S6, after the particle cells are agglomerated, solidifying the hydrogel in the sample by utilizing a solidifying module, and finishing the manufacturing of the shell layer structure.
9. The method of recombining shell structures of cells of claim 8, wherein: the pretreatment specifically comprises the following steps: human ovarian granulosa cells with an adherent area of more than 85% were trypsinized, centrifuged and resuspended in 1ml of 5% hydrogel in a 4ml petri dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110190011.0A CN112958015B (en) | 2021-02-18 | 2021-02-18 | System and method for recombining shell structure by bubble-assisted sound wave |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110190011.0A CN112958015B (en) | 2021-02-18 | 2021-02-18 | System and method for recombining shell structure by bubble-assisted sound wave |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112958015A CN112958015A (en) | 2021-06-15 |
| CN112958015B true CN112958015B (en) | 2022-01-28 |
Family
ID=76285128
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110190011.0A Active CN112958015B (en) | 2021-02-18 | 2021-02-18 | System and method for recombining shell structure by bubble-assisted sound wave |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112958015B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113976051B (en) * | 2021-11-15 | 2022-08-23 | 国科温州研究院(温州生物材料与工程研究所) | From showy aquogel preparation facilities based on micro-fluidic |
| WO2023102818A1 (en) * | 2021-12-09 | 2023-06-15 | 中国科学院深圳先进技术研究院 | Acoustic microfluidic system for cell fusion and preparation method therefor and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105136763A (en) * | 2015-09-10 | 2015-12-09 | 大连海事大学 | Single microalgae cell activity dynamic monitoring novel method and device based on gas-liquid interface single cell capture and chlorophyll fluorescence characterization |
| CN106079677A (en) * | 2016-06-14 | 2016-11-09 | 中国科学院化学研究所 | A kind of patterning Two-Dimensional Bubble array and its preparation method and application |
| CN111774105A (en) * | 2020-06-09 | 2020-10-16 | 南京航空航天大学 | Ultrasonic precise micro-fluidic chip based on nano motor array and implementation method thereof |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE0200860D0 (en) * | 2002-03-20 | 2002-03-20 | Monica Almqvist | Microfluidic cell and method for sample handling |
| CN116179323A (en) * | 2011-05-27 | 2023-05-30 | 不列颠哥伦比亚大学 | Microfluidic cell capture and analysis device for high throughput analysis |
| CN105214742B (en) * | 2015-10-10 | 2017-10-31 | 中国科学院深圳先进技术研究院 | The method of microfluid system and manipulation particulate based on artificial structure's sound field |
| US10982181B2 (en) * | 2016-09-07 | 2021-04-20 | Triad National Security, Llc | Devices for cell culture and methods of making and using the same |
| CN108845026B (en) * | 2018-03-07 | 2020-11-24 | 中国科学院深圳先进技术研究院 | Acoustic surface wave micro-fluidic chip and micron-sized acoustic focal region forming method |
| CN110272860B (en) * | 2019-06-30 | 2020-07-14 | 东南大学苏州医疗器械研究院 | Construction method and application of three-dimensional cell culture microenvironment |
| CN110624427B (en) * | 2019-10-28 | 2020-08-04 | 西安交通大学 | Bubble generation device and method based on surface acoustic wave micro-fluidic |
-
2021
- 2021-02-18 CN CN202110190011.0A patent/CN112958015B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105136763A (en) * | 2015-09-10 | 2015-12-09 | 大连海事大学 | Single microalgae cell activity dynamic monitoring novel method and device based on gas-liquid interface single cell capture and chlorophyll fluorescence characterization |
| CN106079677A (en) * | 2016-06-14 | 2016-11-09 | 中国科学院化学研究所 | A kind of patterning Two-Dimensional Bubble array and its preparation method and application |
| CN111774105A (en) * | 2020-06-09 | 2020-10-16 | 南京航空航天大学 | Ultrasonic precise micro-fluidic chip based on nano motor array and implementation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112958015A (en) | 2021-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112958015B (en) | System and method for recombining shell structure by bubble-assisted sound wave | |
| Kaynak et al. | Addressable acoustic actuation of 3D printed soft robotic microsystems | |
| Baudoin et al. | Folding a focalized acoustical vortex on a flat holographic transducer: Miniaturized selective acoustical tweezers | |
| CN103009632B (en) | Microarray die-free forming device based on surface acoustic wave and forming method | |
| CN107583696B (en) | A kind of particle capture and release device based on bulk acoustic wave excitation and moving bubble | |
| CN105818383B (en) | A kind of Meta Materials photocuring 3D printing method based on holographic optical tweezer | |
| Tang et al. | On-chip simultaneous rotation of large-scale cells by acoustically oscillating bubble array | |
| CN104698516B (en) | The preparation method of curved compound eye lens arra | |
| Zhang et al. | A manta ray-inspired biosyncretic robot with stable controllability by dynamic electric stimulation | |
| CN112899158A (en) | Micro-processing gas matching layer modulation body ultrasonic cell assembling and arranging device, preparation method and application | |
| Sun et al. | A novel three-dimensional microfluidic platform for on chip multicellular tumor spheroid formation and culture | |
| CN112371065B (en) | Method for generating magnetic core-shell microcapsules based on surface acoustic wave microfluidic device | |
| Liu et al. | Effects of two surface acoustic wave sorting chips on particles multi-level sorting | |
| CN108397373A (en) | A kind of valveless electromagnetism Micropump and preparation method thereof | |
| CN114774275A (en) | Three-dimensional cytosphere generating chip based on bipolar electrode under rotating electric field and application | |
| Liu et al. | Multi-level separation of particles using acoustic radiation force and hydraulic force in a microfluidic chip | |
| Ni et al. | Recent Progress on the Development of Magnetically‐Responsive Micropillars: Actuation, Fabrication, and Applications | |
| CN107824105B (en) | Micro mixer capable of adjusting solution concentration gradient based on moving bubbles | |
| Shakeri et al. | The fabrication and bonding of thermoplastic microfluidics: A review | |
| Sun et al. | Dynamic fabrication of microfluidic systems for particles separation based on optical projection lithography | |
| Chen et al. | Hydrodynamic metamaterials: Principles, experiments, and applications | |
| CN202986108U (en) | Micro-array mould-free forming device based on surface acoustic wave | |
| Deng et al. | Fabrication of Magnetic Microrobots by Assembly | |
| Wu et al. | Controllable and Scaffold‐Free Formation of 3D Multicellular Architectures Using a Bipolar Electrode Array | |
| Bian et al. | Investigation on the influence of design parameters of streamline flow tubes on pump performance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |