CN112940921B - Quick sampling detection robot of skin disinfection effect - Google Patents
Quick sampling detection robot of skin disinfection effect Download PDFInfo
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- CN112940921B CN112940921B CN202110173787.1A CN202110173787A CN112940921B CN 112940921 B CN112940921 B CN 112940921B CN 202110173787 A CN202110173787 A CN 202110173787A CN 112940921 B CN112940921 B CN 112940921B
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Abstract
The invention discloses a rapid sampling detection robot for skin disinfection effect, which comprises a box body, wherein a sampling area, a pretreatment area and an incubation detection area are sequentially arranged in the box body from left to right; in the sampling area, a notch is formed in the lower left corner of the box body, a consumable module is arranged on one side of the notch, and the consumable module is arranged at the bottom of the box body; in the pretreatment area, a bearing plate is arranged on the rear side wall of the box body, and a sprayer is arranged above the bearing plate; in the temperature incubation detection zone, horizontal sliding connection has vertical storage plate on the trailing flank of box, storage plate's preceding even a plurality of storage grooves that are used for placing the diaphragm box of having seted up, set up the pull mouth that can wear out storage plate on the right side wall of box, the last heater that is provided with of storage plate. The invention uses a sampling membrane to carry out impression collection on the skin of a subject, the sampled membrane is put into a membrane box for incubation, and finally a camera is used for shooting the growth result after the growth state of the bacterial colony reaches the detection standard.
Description
Technical Field
The invention relates to the field of experimental equipment, in particular to a robot for rapidly sampling and detecting a skin disinfection effect.
Background
The microorganism detection informatization is one of the future development directions of automatic instruments, detection result information which is limited in a small part of system in the past and has fixed updating frequency and slow speed is converted into a form which is updated in real time and is relatively transparent, the use value of the information can be improved in a new layer, and therefore the information can be effectively served to the society. By developing and applying the automatic robot, the basic point for generating basic information can be changed from a human to an instrument, the efficiency is improved, the cost is reduced, and the stability and the accuracy of data are maintained, so that the automatic detection robot has high research value.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provide a rapid sampling and detecting robot with a skin disinfection effect.
Therefore, the invention provides a rapid sampling detection robot for skin disinfection effect, which comprises a box body, wherein a sampling area, a pretreatment area and an incubation detection area are sequentially arranged in the box body from left to right; in the sampling area, a notch for extending into a collected sample is formed in the lower left corner of the box body, a consumable module is arranged on one side of the notch and arranged at the bottom of the box body, and the consumable module is used for placing a membrane; in the pretreatment area, a bearing plate is arranged on the rear side wall of the box body and used for placing the membrane box, and a sprayer is arranged above the bearing plate; in the incubation detection area, a longitudinal storage plate is horizontally connected to the rear side face of the box body in a sliding manner, a plurality of storage grooves used for placing the diaphragm boxes are uniformly formed in the front of the storage plate, a drawing opening capable of penetrating through the storage plate is formed in the right side wall of the box body, and a heater is arranged on the storage plate; the left side of the top of the box body is provided with a first camera and a first position moving mechanism, the first camera is located right above the notch, the output end of the first position moving mechanism is connected with a sampling head through a first electric expansion piece, the output end of the first position moving mechanism can horizontally move, the left side of the top of the box body is provided with a second position moving mechanism, the output end of the second position moving mechanism is connected with a clamping mechanism through a second electric expansion piece, and the output end of the second position moving mechanism can horizontally move; when a sample enters the box body after being shot by the first camera, the first position moving mechanism enables the sampling head to extract the diaphragm from the consumable module, collects the sample on the diaphragm, finally places the diaphragm in the diaphragm box of the bearing plate, and the second position moving mechanism enables the diaphragm box to move from the bearing plate to the accommodating groove.
Further, the sampling head comprises: the cylinder is longitudinally arranged below the first electric expansion piece, and the top of the cylinder is closed and connected with the expansion end of the first electric expansion piece; the cylinder is longitudinally arranged inside the cylinder, the outer surface of the cylinder is attached to the inner surface of the cylinder, the height of the cylinder is smaller than that of the cylinder, and the top of the cylinder is connected with the top of the cylinder through a third electric expansion piece; when the sampling head extracts the membrane, the bottom of the cylinder is in contact with the membrane, the cylinder moves upwards through the third electric expansion piece, and the membrane is adsorbed; when the sampling head is used for placing the membrane, the third electric expansion piece moves the cylinder downwards to separate the membrane from the bottom of the cylinder.
Furthermore, a pressure sensor and a light-emitting point are arranged at the bottom of the cylinder; when the sampling head collects a sample on the diaphragm, the stretching degree of the first electric telescopic device is controlled according to the pressure value detected by the pressure sensor, and meanwhile, the light emitting point downwards emits a light column to the surface of the sample after absorbing the diaphragm.
Furthermore, a placing opening for placing the diaphragm box by a user is formed in the wall of the box body above the bearing plate.
Further, the first position moving mechanism and the second position moving mechanism are consistent in structure;
the first position moving mechanism includes: the supporting frame is fixed at the top of the box body, and the bottom of the supporting frame is a square frame; the number of the sliding blocks is 4, each sliding block is respectively connected to one side edge of the square frame at the bottom of the support frame in a sliding mode, and the sliding of each sliding block is respectively driven through a driving mechanism; the moving block is arranged in the center of a square frame at the bottom of the support frame, the outer surface of each side of the periphery of the moving block is connected with one sliding block through a telescopic rod with variable length, and the bottom of the moving block is connected with the first electric expansion piece; the sliding block slides along the side edge of the square frame at the bottom of the supporting frame under the action of the driving mechanism, so that the position of the moving block is changed.
Further, a handle is arranged on the right side of the containing plate, and the heater is located on the surface of the containing plate.
Furthermore, a second camera is arranged on the wall of the box body opposite to the containing plate, and data shot by the second camera is displayed on a display located outside the box body.
Further, the inside of diaphragm box is provided with the culture medium, the composition of culture medium includes: 17.8% of tryptone, 26% of agar powder, 7% of beef extract, 9% of yeast extract, 9% of glucose, 0.6% of lecithin, 0.6% of vitamin B complex and 60.30% of Tween, wherein the pH value of the culture medium is between 7.0 and 7.5.
The rapid sampling detection robot for the skin disinfection effect provided by the invention has the following beneficial effects:
firstly, a sampling membrane is used for carrying out impression collection on the skin of a subject, then the sampled membrane is placed in a membrane box for incubation, and finally, a camera is used for shooting a growth result after the growth state of a bacterial colony reaches a detection standard; according to the invention, each step required in a single skin disinfection effect (microorganism residual) detection experiment is replaced by an automatic instrument instead of manual operation, and each instrument is combined into a miniature automatic assembly line, so that a single intelligent detection instrument capable of automatically detecting the full-flow skin disinfection effect is finally obtained.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the overall structure of the present invention;
FIG. 3 is a schematic view of the overall structure of the present invention;
FIG. 4 is a schematic view of the overall structure of the present invention;
fig. 5 is a schematic view of the overall structure of the present invention.
Detailed Description
An embodiment of the present invention will be described in detail below with reference to the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the embodiment.
In the present application, the type and structure of components that are not specified are all the prior art known to those skilled in the art, and those skilled in the art can set the components according to the needs of the actual situation, and the embodiments of the present application are not specifically limited.
Specifically, as shown in fig. 1 to 5, an embodiment of the present invention provides a rapid sampling and detecting robot for skin disinfection effect, which includes a box body, and a sampling area, a pretreatment area, and an incubation detection area are sequentially disposed inside the box body 1 from left to right. Wherein, the sampling area is used for collecting the collected sample, the pretreatment area is used for pretreating the collected sample before detection, and the incubation detection area is used for carrying out constant-temperature cultivation on the sample.
In the sampling district, the breach that is used for stretching into the collection sample is offered to the lower left corner of box 1, one side of breach is provided with consumptive material module 2, consumptive material module 2 sets up box 1's bottom, consumptive material module 2 is used for placing the diaphragm. The patch is used for dipping the collected sample, and the cells for obtaining the collected sample are arranged on the patch.
In the preliminary treatment district, be provided with loading board 3 on the rear side wall of box 1, loading board 3 is used for placing diaphragm box 4, the top of loading board 3 is provided with atomizer 5. The sprayer 5 sprays the membrane cassette 4 on the carrier plate 3, which allows the creation of a culture environment, which allows the cells to have a good living environment, and the sprayed solution can be physiological saline.
In the incubation detection zone, horizontal sliding connection has fore-and-aft storage plate 6 on the trailing flank of box 1, and storage plate 6 is preceding even set up a plurality of storage tank 7 that are used for placing diaphragm box 4, set up on the right side wall of box 1 and to wear out storage plate 6's pull mouth, be provided with the heater on storage plate 6. The set temperature is reached by heating, so that a proper temperature can be provided for the growth of the cells, and the cells can stably grow.
The sampling area, the pretreatment area and the incubation detection area are connected, and the collection, treatment and culture of cells for collecting samples are completed through mutual direct sequential matching, specifically, a first camera 8 and a first position moving mechanism 9 are arranged on the left side of the top of the box body 1, the first camera 8 is positioned right above the gap, the output end of the first position moving mechanism 9 is connected with a sampling head 11 through a first electric expansion piece 10, the output end of the first position moving mechanism 9 can horizontally move, a second position moving mechanism 12 is arranged on the left side of the top of the box body 1, the output end of the second position moving mechanism 12 is connected with a clamping mechanism through a second electric expansion piece 13, and the output end of the second position moving mechanism 12 can horizontally move; when a sample enters the box body 1 after being shot by the first camera 8, the first position moving mechanism 9 enables the sampling head 11 to extract a diaphragm in the consumable module 2, collects the sample on the diaphragm, finally places the diaphragm in the diaphragm box 4 of the bearing plate 3, and the diaphragm box 4 is moved to the accommodating groove 7 from the bearing plate 3 through the second position moving mechanism 12.
In this embodiment, the sampling head 11 includes: a cylinder 11-1 and a cylinder 11-2. The cylinder 11-1 is longitudinally arranged below the first electric expansion piece 10, and the top of the cylinder is closed and connected with the expansion end of the first electric expansion piece 10; the cylinder 11-2 is longitudinally arranged inside the cylinder 11-1, the outer surface of the cylinder is attached to the inner surface of the cylinder 11-1, the height of the cylinder is smaller than that of the cylinder 11-1, and the top of the cylinder is connected with the top of the cylinder 11-1 through a third electric expansion piece 11-3; when the sampling head 11 extracts the membrane, the bottom of the cylinder 11-1 is contacted with the membrane, and the cylinder 11-2 is moved upwards by the third electric expansion piece 11-3 to adsorb the membrane; when the sampling head 11 places the membrane, the third electric telescopic device 11-3 moves the cylinder 11-2 downwards to separate the membrane from the bottom of the cylinder 11-1.
Among the above-mentioned technical scheme, adsorb the diaphragm through the mode of adsorbing for the diaphragm is on sampling head 11, thereby accomplishes the process of sampling. When the adsorption is not needed, the release is needed, and the adsorption and the release of the invention are carried out in a pneumatic mode.
Meanwhile, the bottom of the cylinder 11-1 is provided with a pressure sensor 11-4 and a light-emitting point 11-5; when the sampling head 11 collects a sample on the diaphragm, the expansion degree of the first electric expansion piece 10 is controlled according to the pressure value detected by the pressure sensor 11-4, and meanwhile, the light emitting point 11-5 downwards emits a light column to the surface of the sample after absorbing the diaphragm. The light-emitting point is used for emitting a light column downwards after the diaphragm is sucked, the light column is reflected on the skin of a subject to indicate the position corresponding to the current diaphragm, and the pressure sensor is used for monitoring the pressure between the diaphragm and the skin of the subject in real time in the sampling process.
In this embodiment, the wall of the box body 1 above the bearing plate 3 is provided with a placing opening for a user to place the diaphragm box 4. Therefore, the membrane box 4 can be conveniently placed from the outer side of the box body 1 by a user, the use is more convenient, and meanwhile, the replacement and the processing of the membrane box 4 are more convenient.
In the present embodiment, the first position moving mechanism 9 and the second position moving mechanism 12 are identical in structure; the first position moving mechanism 9 includes: the device comprises a support frame 9-1, a sliding block 9-2 and a moving block 9-3, wherein the support frame 9-1 is fixed at the top of the box body 1, and the bottom of the support frame is a square frame; the number of the sliding blocks 9-2 is 4, each sliding block 9-2 is respectively connected to one side edge of the bottom square frame of the supporting frame 9-1 in a sliding manner, and the sliding of each sliding block 9-2 is respectively driven by a driving mechanism; the moving block 9-3 is arranged at the center of a square frame at the bottom of the support frame 9-1, the outer surface of each side of the periphery of the moving block is connected with one sliding block 9-2 through a telescopic rod 9-4 with variable length, and the bottom of the moving block 9-3 is connected with the first electric expansion piece 10; the slide block 9-2 slides along the side edge of the bottom square frame of the support frame 9-1 under the action of the driving mechanism, so that the position of the moving block 9-3 is changed.
In this embodiment, a handle 14 is provided on the right side of the receiving plate 6, and the heater is located on the surface of the receiving plate 6. Just so can be very convenient take in board 6 pull out to use and observe diaphragm box 4, very convenience.
In this embodiment, a second camera 15 is disposed on a wall of the box body 1 opposite to the containing plate 6, data captured by the second camera 15 is displayed on a display 16 located outside the box body 1, the second camera 15 uses a camera with not less than 30 ten thousand pixels to photograph the cultured membrane, and simultaneously, a program is used to analyze the number and volume of color blocks in the picture, and finally, the number of colonies corresponding to each membrane is obtained through machine analysis.
In this embodiment, the membrane cassette 4 is provided with a culture medium therein, and the culture medium comprises the following components: 17.8% of tryptone, 26% of agar powder, 7% of beef extract, 9% of yeast extract, 9% of glucose, 0.6% of lecithin, 0.6% of vitamin B complex and 60.30% of Tween, wherein the pH value of the culture medium is between 7.0 and 7.5.
The membrane is divided into three layers, the blue layer surface at the bottommost layer is a water-proof plastic package layer, and the water-proof plastic package layer is attached to filter paper in advance when the sampling membrane is manufactured. The red layer of the middle layer is thin layer chromatography filter paper, and the green layer of the top layer is solid culture medium.
The culture medium used for the patch is a mixture of nutrient substances and cold water gel. The preparation scheme is that the nutrient substances and cold hydrogel are added into deionized water to be constant volume to 1000mL, and 1.25mL of 1mg/mL sodium deoxycholate, 8mL of 1.6% bromocresol purple solution and 5mL of 0.8% TTC solution are added after sterilization is carried out for 15min at 121 ℃.
The nutrient substance in the culture medium comprises, per liter of culture medium, tryptone 15.0g, beef extract 5.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 5.0g, and lactose 15.0 g. The optimal range of the pH value of the culture medium is 6.3-6.5.
The optimal proportion of the cold hydrogel is that 0.7g of cold hydrogel is added into each liter of culture medium, and the specific formula is as follows: the mass volume ratio of the components is 0.2-0.4 percent of sodium polyacrylate, 0.1-0.3 percent of xanthan gum, 0.1-0.2 percent of carrageenan and 0.1-0.2 percent of konjac glucomannan.
The concentration of TTC should be adjusted to 0.4 mg/L.
The diaphragm manufacturing scheme is as follows: firstly, performing plastic packaging treatment on one surface of qualified thin-layer chromatography filter paper, adding a waterproof coating layer on the surface, then cutting the filter paper into a required shape according to a standard or a requirement, paving the filter paper in a container such as a glass plate, pouring a prepared culture medium into the container to soak the filter paper, taking out the filter paper after 2 hours, drying the filter paper in the container at 37 ℃, removing the culture medium remained on the plastic packaging side, subpackaging the membrane in a sterilized polypropylene plastic bag, sealing and refrigerating for later use, and waiting for subsequent treatment.
The cold hydrogel is a substance which can be used for replacing agar in a common culture medium, so that the defect that the agar is easy to solidify at low temperature can be overcome, and the texture of the finished product film is more compact and uniform.
In the invention, the formula of the culture medium is as follows (under an ideal state): 17.8% of tryptone, 26% of agar powder, 7% of beef extract, 9% of yeast extract, 9% of glucose, 0.6% of lecithin, 0.6% of vitamin B complex and 6030% of tween. Mixing the above materials at a certain proportion, adding into deionized water at a ratio of 61 g/L, adjusting pH to 7.0-7.5, autoclaving, and pouring into a carrying box. The finished product requires no bubble after the culture medium is solidified, the surface is smooth and flat, the thickness is uniform, and the average thickness is 1 mm.
The above disclosure is only for a few specific embodiments of the present invention, however, the present invention is not limited to the above embodiments, and any variations that can be made by those skilled in the art are intended to fall within the scope of the present invention.
Claims (6)
1. The rapid sampling and detecting robot for the skin disinfection effect comprises a box body and is characterized in that a sampling area, a pretreatment area and an incubation detection area are sequentially arranged in the box body (1) from left to right;
in the sampling area, a notch used for extending into a collected sample is formed in the lower left corner of the box body (1), a consumable module (2) is arranged on one side of the notch, the consumable module (2) is arranged at the bottom of the box body (1), and the consumable module (2) is used for placing a membrane;
in the pretreatment area, a bearing plate (3) is arranged on the rear side wall of the box body (1), the bearing plate (3) is used for placing the membrane box (4), and a sprayer (5) is arranged above the bearing plate (3);
in the incubation detection area, a longitudinal storage plate (6) is horizontally connected to the rear side face of the box body (1) in a sliding mode, a plurality of storage grooves (7) used for placing the membrane boxes (4) are uniformly formed in the front face of the storage plate (6), a drawing opening capable of penetrating through the storage plate (6) is formed in the right side wall of the box body (1), and a heater is arranged on the storage plate (6);
a first camera (8) and a first position moving mechanism (9) are arranged on the left side of the top of the box body (1), the first camera (8) is located right above the notch, the output end of the first position moving mechanism (9) is connected with a sampling head (11) through a first electric expansion piece (10), the output end of the first position moving mechanism (9) can move horizontally, a second position moving mechanism (12) is arranged on the left side of the top of the box body (1), the output end of the second position moving mechanism (12) is connected with a clamping mechanism through a second electric expansion piece (13), and the output end of the second position moving mechanism (12) can move horizontally;
when a sample enters the box body (1) through shooting by the first camera (8), the first position moving mechanism (9) enables the sampling head (11) to extract a diaphragm in the consumable module (2), collects the sample on the diaphragm, finally places the diaphragm in the diaphragm box (4) of the bearing plate (3), and then moves the diaphragm box (4) from the bearing plate (3) to the accommodating groove (7) through the second position moving mechanism (12);
the sampling head (11) comprises:
the cylinder (11-1) is longitudinally arranged below the first electric expansion piece (10), and the top of the cylinder is closed and connected with the expansion end of the first electric expansion piece (10);
the cylinder (11-2) is longitudinally arranged inside the cylinder (11-1), the outer surface of the cylinder is attached to the inner surface of the cylinder (11-1) and is lower than the cylinder (11-1), and the top of the cylinder is connected with the top of the cylinder (11-1) through a third electric expansion piece (11-3);
when the sampling head (11) extracts the membrane, the bottom of the cylinder (11-1) is contacted with the membrane, and the cylinder (11-2) is moved upwards through the third electric expansion piece (11-3) to adsorb the membrane; when the sampling head (11) is used for placing a membrane, the third electric expansion piece (11-3) is used for moving the cylinder (11-2) downwards to separate the membrane from the bottom of the cylinder (11-1);
and a second camera (15) is arranged on the wall of the box body (1) opposite to the containing plate (6), and data shot by the second camera (15) is displayed on a display (16) positioned outside the box body (1).
2. The robot for rapid sampling and detecting of skin disinfection effect according to claim 1, wherein the bottom of said cylinder (11-1) is provided with a pressure sensor (11-4) and a light emitting point (11-5);
when the sampling head (11) collects a sample on the diaphragm, the stretching degree of the first electric telescopic device (10) is controlled according to the pressure value detected by the pressure sensor (11-4), and meanwhile, the light emitting point (11-5) downwards emits a light column to the surface of the sample after absorbing the diaphragm.
3. The robot for rapidly sampling and detecting the skin disinfection effect according to claim 1, wherein a placing through hole for a user to place the membrane cassette (4) is formed on the wall of the box body (1) above the bearing plate (3).
4. The robot for rapid sampling and detecting of skin disinfection effect according to claim 1, wherein said first position moving mechanism (9) and said second position moving mechanism (12) are identical in structure;
the first position moving mechanism (9) includes:
the supporting frame (9-1) is fixed at the top of the box body (1), and the bottom of the supporting frame is a square frame;
the number of the sliding blocks (9-2) is 4, each sliding block (9-2) is respectively connected to one side edge of the bottom square frame of the supporting frame (9-1) in a sliding mode, and the sliding of each sliding block (9-2) is respectively driven through a driving mechanism;
the moving block (9-3) is arranged in the center of a square frame at the bottom of the supporting frame (9-1), the outer surface of each side of the periphery of the moving block is connected with one sliding block (9-2) through a length-variable telescopic rod (9-4), and the bottom of the moving block (9-3) is connected with the first electric expansion piece (10);
the sliding block (9-2) slides along the side edge of the bottom square frame of the supporting frame (9-1) under the action of the driving mechanism, so that the position of the moving block (9-3) is changed.
5. The robot for rapidly sampling and detecting skin disinfection effects according to claim 1, wherein a handle (14) is provided on the right side of the receiving plate (6), and the heater is located on the surface of the receiving plate (6).
6. The robot for rapidly sampling and detecting the skin disinfection effect as claimed in claim 1, wherein a culture medium is arranged inside the membrane cassette (4), and the culture medium comprises the following components: 17.8% of tryptone, 26% of agar powder, 7% of beef extract, 9% of yeast extract, 9% of glucose, 0.6% of lecithin, 0.6% of vitamin B complex and 60.30% of Tween, wherein the pH value of the culture medium is between 7.0 and 7.5.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110173787.1A CN112940921B (en) | 2021-02-06 | 2021-02-06 | Quick sampling detection robot of skin disinfection effect |
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| CN202110173787.1A CN112940921B (en) | 2021-02-06 | 2021-02-06 | Quick sampling detection robot of skin disinfection effect |
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| CN112940921A CN112940921A (en) | 2021-06-11 |
| CN112940921B true CN112940921B (en) | 2022-08-02 |
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| CA2449925C (en) * | 2001-06-08 | 2010-03-09 | F. Hoffmann-La Roche Ag | Control solution packets and methods for calibrating bodily fluid sampling devices |
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| CN103356243A (en) * | 2012-03-27 | 2013-10-23 | 马广 | Novel skin sampler |
| CN203290876U (en) * | 2013-03-22 | 2013-11-20 | 北京德麦特捷康科技发展有限公司 | Skin image collection device |
| ITMI20130692A1 (en) * | 2013-04-26 | 2014-10-27 | Copan Italia Spa | DEVICE AND PROCEDURE FOR THE AUTOMATIC PROCESSING OF CROP PLATES FOR MICROBIOLOGICAL SAMPLES |
| CN107661124A (en) * | 2017-09-22 | 2018-02-06 | 陈昭斌 | A kind of skin degerming effect monitoring quick sampling device |
| CN108872143A (en) * | 2018-05-22 | 2018-11-23 | 南京农业大学 | A kind of wheat infection head blight level detection method based near infrared spectrum |
| CN109222897B (en) * | 2018-08-01 | 2024-06-18 | 中日友好医院 | Pressure measuring device for probe of skin confocal microscope |
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| CN209549188U (en) * | 2018-08-30 | 2019-10-29 | 南京工业大学 | Quick serum extractor |
| CN110870766A (en) * | 2018-08-31 | 2020-03-10 | 宁波戴维医疗器械股份有限公司 | Data communication device for incubator and incubator |
| GB201816911D0 (en) * | 2018-10-17 | 2018-11-28 | Univ London Queen Mary | Apparatus and methods |
| CN110093269A (en) * | 2019-06-13 | 2019-08-06 | 王春梅 | A kind of sampling of microorganisms in water, culture and detection integrated apparatus |
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