CN112940292A - Dendrobium officinale polysaccharide hydrogel as well as preparation method and application thereof - Google Patents
Dendrobium officinale polysaccharide hydrogel as well as preparation method and application thereof Download PDFInfo
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 132
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 132
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 132
- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 88
- 239000000017 hydrogel Substances 0.000 title claims abstract description 70
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000000843 powder Substances 0.000 claims abstract description 49
- 239000000499 gel Substances 0.000 claims abstract description 18
- 150000007529 inorganic bases Chemical class 0.000 claims abstract description 7
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims description 30
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 22
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical group [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 18
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 16
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 16
- 239000011780 sodium chloride Substances 0.000 claims description 11
- 239000001103 potassium chloride Substances 0.000 claims description 9
- 235000011164 potassium chloride Nutrition 0.000 claims description 9
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 8
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 8
- 230000007935 neutral effect Effects 0.000 claims description 4
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims description 4
- 159000000000 sodium salts Chemical class 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 2
- 241000026010 Dendrobium candidum Species 0.000 abstract description 55
- 239000002994 raw material Substances 0.000 abstract description 13
- 239000003431 cross linking reagent Substances 0.000 abstract description 2
- 238000011112 process operation Methods 0.000 abstract description 2
- 239000012153 distilled water Substances 0.000 description 14
- 239000000203 mixture Substances 0.000 description 12
- 239000000047 product Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000003519 biomedical and dental material Substances 0.000 description 2
- 238000002329 infrared spectrum Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 241001523681 Dendrobium Species 0.000 description 1
- 208000019693 Lung disease Diseases 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 201000007100 Pharyngitis Diseases 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000002612 dispersion medium Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000018927 edible plant Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/24—Acids; Salts thereof
- C08K3/26—Carbonates; Bicarbonates
- C08K2003/262—Alkali metal carbonates
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/16—Halogen-containing compounds
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/20—Oxides; Hydroxides
- C08K3/22—Oxides; Hydroxides of metals
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a dendrobium officinale polysaccharide hydrogel as well as a preparation method and application thereof. The raw materials for preparing the dendrobium officinale polysaccharide hydrogel comprise 1-3 wt% of dendrobium officinale polysaccharide powder, 0.1-1.5 wt% of inorganic base, 0-0.5 wt% of inorganic salt and the balance of water. According to the invention, the dendrobium candidum polysaccharide is prepared into hydrogel for the first time under the condition of no cross-linking agent, and the gel strength of the prepared dendrobium candidum polysaccharide hydrogel is 100-130g/cm2The water holding rate is 85-95%. Provides a new approach for the development of dendrobium officinale polysaccharide products, and has wide application and high economic value. The inventionThe prepared dendrobium officinale hydrogel has good water holding rate and gel strength, has biocompatibility and biodegradability, and can be used as a biochemical material; meanwhile, the preparation method disclosed by the invention is simple in process operation, low in cost and easy to realize industrialization.
Description
Technical Field
The invention relates to a dendrobium officinale polysaccharide hydrogel as well as a preparation method and application thereof, belonging to the technical field of biochemical materials.
Background
The hydrogel is a macromolecular network system taking water as a dispersion medium, has certain elasticity, can keep a certain shape in water, and can keep a large amount of water. The hydrogel is widely applied to the fields of biochemistry, medicine, agriculture, food, daily chemical industry and the like. To date, most hydrogels are non-biodegradable, which limits their use in medicine and environmental protection.
Dendrobium officinale (Dendrobium officinale Kimura et Migo) is an edible plant of the genus Dendrobium of the family Orchidaceae with high medicinal value. As a traditional Chinese medicine, it shows various biological activities medically, including treating hyperglycemia, lung diseases, throat inflammation and the like. The main products of dendrobium officinale in the current market are: fresh dendrobium officinale strips, dendrobium officinale capsules and dendrobium officinale powder, and no report is found on the development of other forms of dendrobium officinale products.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides a dendrobium officinale polysaccharide product which is biodegradable, has good biocompatibility and can be used as a biomedical material.
In order to solve the problems, the invention provides a dendrobium officinale polysaccharide hydrogel which comprises 1-3% of dendrobium officinale polysaccharide powder, 0.1-1.5% of inorganic base, 0-0.5% of neutral inorganic salt and the balance of water.
Preferably, the dendrobium officinale polysaccharide powder is a polysaccharide substance extracted from stems and/or leaves of dendrobium officinale.
Preferably, the inorganic base is at least one of sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide.
Preferably, the neutral inorganic salt is at least one of inorganic sodium salt and inorganic potassium salt.
More preferably, the inorganic sodium salt is sodium chloride and the inorganic potassium salt is potassium chloride.
Preferably, the water is distilled water or deionized water.
The invention also provides a preparation method of the dendrobium officinale polysaccharide hydrogel, which comprises the following steps:
step 1: dispersing the dendrobium officinale polysaccharide powder in water, and standing to fully swell the dendrobium officinale polysaccharide powder into uniform sol;
step 2: adding inorganic base and inorganic salt into the sol, stirring uniformly at a constant temperature, and standing to obtain the dendrobium officinale polysaccharide hydrogel.
Preferably, the standing conditions in the step 1 are as follows: the time is 1-2h, and the temperature is 20-30 ℃.
Preferably, the stirring time in the step 2 is 5-20min, the stirring temperature at constant temperature is 20-60 ℃, and the standing time is 12-24 h.
Preferably, the gel strength of the dendrobium officinale polysaccharide hydrogel obtained in the step 2 is 100-130g/cm2The water holding rate is 85-95%.
The invention also provides application of the dendrobium officinale polysaccharide hydrogel in biomedical materials.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the dendrobium candidum polysaccharide is prepared into hydrogel under the condition of no cross-linking agent for the first time, a new way is provided for the development of dendrobium candidum polysaccharide products, and the dendrobium candidum polysaccharide product has wide application and high economic value.
2. The dendrobium officinale hydrogel disclosed by the invention has good water holding rate and gel strength, has biocompatibility and biodegradability, and can be used as a biomedical material; meanwhile, the preparation method disclosed by the invention is simple in process operation, low in cost and easy to realize industrialization.
Drawings
FIG. 1 is a graph showing the texture change trend of an aqueous gel of Dendrobium officinale Kimura et Migo prepared according to an embodiment of the present invention;
FIG. 2 is an appearance diagram of the Dendrobium officinale polysaccharide hydrogel prepared in example 3;
fig. 3 is an infrared spectrum of the dendrobium officinale polysaccharide hydrogel prepared in example 3.
Detailed Description
In order to make the invention more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings.
Example 1
Preparing dendrobium officinale polysaccharide powder:
weighing 45g of dried and crushed dendrobium officinale stems, adding 10 times of volume of absolute ethyl alcohol, soaking at normal temperature for 72h, removing the ethyl alcohol, adding 20 times of volume of distilled water, extracting at 100 ℃ for 2h, repeatedly extracting for 4 times, carrying out rotary evaporation concentration on an extracting solution, adding 4 times of volume of absolute ethyl alcohol, standing for 24h, centrifuging (3000rpm for 15min), collecting precipitates, freezing and drying the precipitates, and crushing to obtain the dendrobium officinale polysaccharide powder.
The dendrobium officinale polysaccharide powder used in the following embodiments is prepared by the method of the embodiment.
Example 2
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to enable the dendrobium candidum polysaccharide powder to be fully swelled into uniform sol, simultaneously adding potassium carbonate and sodium chloride into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 1 wt% of potassium carbonate, 0.5 wt% of sodium chloride and 97 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 132g/cm2The water retention rate was 93%.
Example 3
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to enable the dendrobium candidum polysaccharide powder to be fully swelled into uniform sol, simultaneously adding potassium carbonate and potassium chloride into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 1 wt% of potassium carbonate, 0.5 wt% of sodium chloride and 97 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and has the gel strength of 139g/cm2The water retention rate was 94%.
Fig. 2 is an appearance diagram of the dendrobium officinale polysaccharide hydrogel prepared in this example, and fig. 3 is an infrared spectrum diagram of the dendrobium officinale polysaccharide hydrogel prepared in this example, from which it can be seen that acetyl groups of dendrobium officinale polysaccharide are hydrolyzed under alkaline conditions.
Example 4
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to enable the dendrobium candidum polysaccharide powder to be fully swelled into uniform sol, adding potassium carbonate into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and then standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 1% of potassium carbonate and 97.5 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 123g/cm2The water retention rate was 92%.
Example 5
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to fully swell the dendrobium candidum polysaccharide powder into uniform sol, adding sodium carbonate and sodium chloride into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium candidum polysaccharide powder, 1 wt% of sodium carbonate, 0.5 wt% of sodium chloride and 97 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 121g/cm2Hand holdingThe water content was 93%.
Example 6
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to fully swell the dendrobium candidum polysaccharide powder into uniform sol, adding sodium carbonate and potassium chloride into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium candidum polysaccharide powder, 1 wt% of sodium carbonate, 0.5 wt% of potassium chloride and 97 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 128g/cm2The water retention rate was 93%.
Example 7
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to fully swell the dendrobium candidum polysaccharide powder into uniform sol, adding sodium carbonate into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 1 wt% of sodium carbonate and 97.5 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 109g/cm2The water retention rate was 91%.
Example 8
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to fully swell the dendrobium candidum polysaccharide powder into uniform sol, adding sodium hydroxide and sodium chloride into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 0.5 wt% of sodium hydroxide, 0.5 wt% of sodium chloride and 97.5 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 118g/cm2The water retention rate is 90%.
Example 9
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to fully swell the dendrobium candidum polysaccharide powder into uniform sol, adding sodium hydroxide and potassium chloride into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 0.5 wt% of sodium hydroxide, 0.5 wt% of potassium chloride and 97.5 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 121g/cm2The water retention rate was 91%.
Example 10
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to fully swell the dendrobium candidum polysaccharide powder into uniform sol, adding sodium hydroxide into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 0.5 wt% of sodium hydroxide and 98 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 105g/cm2The water retention rate was 89%.
Example 11
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to fully swell the dendrobium candidum polysaccharide powder into uniform sol, adding potassium hydroxide and sodium chloride into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 0.5 wt% of potassium hydroxide, 0.5 wt% of sodium chloride and 97.5 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 113g/cm2The water retention rate is 90%.
Example 12
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to fully swell the dendrobium candidum polysaccharide powder into uniform sol, adding potassium hydroxide and potassium chloride into the dendrobium candidum polysaccharide sol, stirring for 20 minutes in a water bath at 50 ℃, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 0.5 wt% of potassium hydroxide, 0.5 wt% of potassium chloride and 97.5 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 119g/cm2The water retention rate was 92%.
Example 13
Preparation of a dendrobium officinale polysaccharide hydrogel:
dispersing the dendrobium candidum polysaccharide powder in water under the stirring condition, standing for 2 hours at room temperature to fully swell the dendrobium candidum polysaccharide powder into uniform sol, adding potassium hydroxide into the dendrobium candidum polysaccharide sol, stirring in a water bath at 50 ℃ for 20 minutes, and standing for 24 hours at room temperature (25 ℃) to obtain the dendrobium candidum polysaccharide hydrogel, wherein the mixture ratio of the raw materials is as follows: 1.5 wt% of dendrobium officinale polysaccharide powder, 0.5 wt% of potassium hydroxide and 98 wt% of distilled water.
The prepared dendrobium officinale polysaccharide hydrogel has complete appearance and good elasticity, and the gel strength is 107g/cm2The water retention rate was 89%.
The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way and substantially, it should be noted that those skilled in the art may make several modifications and additions without departing from the scope of the present invention, which should also be construed as a protection scope of the present invention.
Claims (10)
1. The dendrobium officinale polysaccharide hydrogel is characterized by comprising 1-3% of dendrobium officinale polysaccharide powder, 0.1-1.5% of inorganic base, 0-0.5% of neutral inorganic salt and the balance of water.
2. The dendrobium officinale polysaccharide hydrogel according to claim 1, wherein the dendrobium officinale polysaccharide powder is a polysaccharide substance extracted from stems and/or leaves of dendrobium officinale.
3. The dendrobium officinale polysaccharide hydrogel according to claim 1, wherein the inorganic base is at least one of sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide.
4. The dendrobium officinale polysaccharide hydrogel according to claim 1, wherein the neutral inorganic salt is at least one of an inorganic sodium salt and an inorganic potassium salt.
5. The dendrobium officinale polysaccharide hydrogel according to claim 4, wherein the inorganic sodium salt is sodium chloride, and the inorganic potassium salt is potassium chloride.
6. The preparation method of the dendrobium officinale polysaccharide hydrogel according to any one of claims 1 to 5, which is characterized by comprising the following steps:
step 1: dispersing the dendrobium officinale polysaccharide powder in water, and standing to fully swell the dendrobium officinale polysaccharide powder into uniform sol;
step 2: adding inorganic base and inorganic salt into the sol, stirring uniformly at a constant temperature, and standing to obtain the dendrobium officinale polysaccharide hydrogel.
7. The preparation method of the dendrobium officinale polysaccharide hydrogel according to claim 6, wherein the standing condition in the step 1 is as follows: the time is 1-2h, and the temperature is 20-30 ℃.
8. The preparation method of the dendrobium officinale polysaccharide hydrogel according to claim 6, wherein the stirring time in the step 2 is 5-20min, the stirring temperature at constant temperature is 20-60 ℃, and the standing time is 12-24 h.
9. The method for preparing the dendrobium officinale polysaccharide hydrogel as claimed in claim 6, wherein the gel strength of the dendrobium officinale polysaccharide hydrogel obtained in the step 2 is 100-130g/cm2The water holding rate is 85-95%.
10. The use of the dendrobium officinale polysaccharide hydrogel of any one of claims 1-5 in biomedical materials.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114403182A (en) * | 2021-12-31 | 2022-04-29 | 上海应用技术大学 | Chiffon cake containing plant dendrobe polysaccharide and processing method thereof |
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| CN106220747A (en) * | 2016-06-23 | 2016-12-14 | 江西九草铁皮石斛科技协同创新有限公司 | Ultrasonic assistant Vacuum cavitation extracts Dendrobium officinale polysaccharide and the preparation technology of instant powder thereof |
| CN106890122A (en) * | 2017-03-27 | 2017-06-27 | 广州天玺生物科技有限公司 | Skin soothing and itching relieving gel and preparation method thereof |
| US20200281214A1 (en) * | 2017-09-30 | 2020-09-10 | JlANGSU VOCATIONAL COLLEGE OF AGRICULTURE AND FORESTRY | Fusarium solani and use of same in prevention and treatment of southern blight of dendrobium officinale kimura et migo |
| CN108477612A (en) * | 2018-02-06 | 2018-09-04 | 刘利勇 | A kind of dendrobium nobile nutritive powder and preparation method including dendrobium candidum purified extract |
| CN108464960A (en) * | 2018-04-17 | 2018-08-31 | 凯瑞欧（天津）生物技术有限公司 | A kind of dendrobium candidum beauty-care gel |
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| CN110387068A (en) * | 2019-08-01 | 2019-10-29 | 温州大学 | A kind of dendrobium polysaccharide nanofiber hydrogel |
| CN111253495A (en) * | 2020-01-20 | 2020-06-09 | 文山壮族苗族自治州农业科学院 | Extraction process of dendrobe polysaccharide and dendrobine in dendrobium officinale |
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| CN114403182A (en) * | 2021-12-31 | 2022-04-29 | 上海应用技术大学 | Chiffon cake containing plant dendrobe polysaccharide and processing method thereof |
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