CN112915120A

CN112915120A - Magnolia officinalis processed product and processing and detecting method

Info

Publication number: CN112915120A
Application number: CN202110125350.0A
Authority: CN
Inventors: 林昶; 李玮; 杨长福; 王建科; 陈志琳; 吴书亮; 李玉平
Original assignee: Guizhou University of Traditional Chinese Medicine
Current assignee: Guizhou University of Traditional Chinese Medicine
Priority date: 2021-01-29
Filing date: 2021-01-29
Publication date: 2021-06-08

Abstract

The invention relates to a magnolia officinalis processed product and a processing and detecting method, wherein the magnolia officinalis processed product comprises the following components: the ginger juice consists of 2: 1-18: 1, and the processing method comprises the following steps: squeezing rhizoma Zingiberis recens to obtain juice; mixing cortex Magnolia officinalis with succus Zingiberis recens, and oven drying. The invention optimizes the prescription of the processed product of the ginger processed magnolia bark and the processing method thereof through tests, and defines the best method of the ginger processed magnolia bark, and the total content of honokiol and magnolol reaches 3.34 percent at most by using the method; the detection method is determined, and the detection method has good repeatability, stability, precision and the like.

Description

Magnolia officinalis processed product and processing and detecting method

Technical Field

The invention relates to the field of traditional Chinese medicine processing, in particular to a magnolia officinalis processed product and processing and detecting methods.

Background

Magnolia officinalis, also known as Magnolia officinalis, Magnolia officinalis and Magnolia officinalis in the botanical field, is a plant of Magnoliaceae and Magnoliaceae, and commonly comprises Magnolia officinalis (original subspecies) and Magnolia officinalis (subspecies). The polymerized fruit is in a shape of a round oval and 9-15 cm long; the bone-whole line has a beak with a length of 3-4 mm; the seeds are in the shape of a triangle, inverted egg and are about 1cm long. The flowering period is 5-6 months, and the fruit period is 8-10 months. Mainly produced in Sichuan and Hubei provinces. The Chinese medicinal materials refer to dried bark, root bark and branch bark of the plant. Stripping off root bark and branch bark for 4-6 months, directly drying in the shade, slightly boiling the dried bark in boiling water, piling up the dried bark in a shady and humid place, steaming and softening the dried bark until the inner surface of the dried bark is purple brown or tan, taking out the dried bark, rolling the dried bark into a cylinder shape, and drying. Cut into shreds and prepare ginger. Can be used for treating dyspepsia, abdominal distention, constipation, and damp obstruction of middle warmer. Can also be added into cancer medicine. Therefore, how to further enhance the effect of magnolia officinalis is worth intensive study.

In the prior art, the application number is CN200910095729.0, the invention discloses a processing method of a traditional Chinese medicine magnolia officinalis, which comprises the following steps: cleaning cortex Magnolia officinalis, scraping off coarse bark, cleaning, softening at room temperature, cutting into 1cm long shreds, and drying in oven at 50-60 deg.C for 1-1.5 hr to obtain crude product of cortex Magnolia officinalis; squeezing ginger into juice, wherein the using amount of the ginger is 10-15% of that of the raw magnolia officinalis, adding water into the ginger juice for diluting, pouring the diluted ginger juice into the raw magnolia officinalis for stewing for 60-90 minutes, and the mass of the diluted ginger juice is 40-60% of that of the raw magnolia officinalis; the ginger juice magnolia officinalis is placed in a microwave oven, is processed for 15-30 minutes under the power of 400-700W, is taken out and is cooled to obtain the traditional Chinese medicine ginger magnolia officinalis processed product. The method has the advantages of standard processing steps, uniform technical parameters and easily controlled processing process; compared with the traditional ginger magnolia bark processed product, the prepared ginger magnolia bark processed product has small loss of the sum of the contents of the effective components, and can effectively meet the clinical requirement on the ginger magnolia bark decoction pieces. However, this invention has the following problems: 1. the mangnolia officinalis is cut into 1cm long shreds after being softened at normal temperature, and the shreds are placed in an oven at 50-60 ℃ for drying for 1-1.5 hours, so that the effective components of the mangnolia officinalis are easily lost due to direct drying in the oven; 2. the stacking thickness during drying is not clear, and the time is not easy to control; 3. the microwave processing is carried out for 15-30 minutes under the power of 400-700W, and the active ingredients of the mangnolia officinalis processed by the microwave are likely to be lost; the specification shows that the sum of the contents of magnolol and honokiol in the processed product of the traditional Chinese medicine magnolia officinalis is about 2.91 percent, and the effective components are low.

The invention overcomes the technical problem of CN200910095729.0, the shredded magnolia officinalis and ginger juice are uniformly mixed and then dried in an oven, and the magnolia officinalis is not directly dried in the oven, so the content of honokiol and magnolol is effectively reserved, and the test proves that the total content of the honokiol and the magnolol reaches 3.34 percent at most.

The invention discloses a novel processing method of magnolia officinalis with ginger under the application number of CN201610096597.3 in the prior art, which comprises the following steps: pretreating cortex Magnolia officinalis, preparing adjuvant ginger juice, adding ginger juice into cortex Magnolia officinalis in batches, moistening, preheating coarse sand, adding ginger cortex Magnolia officinalis, parching, sweating, and air drying. By adopting the processing method of the magnolia officinalis, the ginger magnolia officinalis can be uniformly heated, the phenomena of scorching and the like can not occur, the generation of oil smoke can be effectively reduced, and the pollution to the environment and the influence on the health of processing workers are reduced; meanwhile, the dissolution rate of the active ingredients of the magnolia officinalis is improved, and the drug effect is enhanced. However, this invention has the following problems: 1. the squeezed ginger juice is heated and boiled after being subjected to ultrasonic treatment, and the treatment process of the ginger juice is complicated; 2. the ginger juice is added into the magnolia officinalis, the heating and moistening are carried out for 6 hours, the sweating is carried out, the processing time is long, the cost is high, and the effective components are easy to lose due to the fact that the heating temperature is not clear; 3. the feasibility of the process was not confirmed by orthogonal experiments.

The invention overcomes the technical problem of CN201610096597.3, and the ginger is directly squeezed into juice, the process is simple; the orthogonal test omits the moistening procedure, the time is shorter, and the test proves that the total content of the honokiol and the magnolol of the invention reaches 3.34 percent at most.

Application number CN201310277299.0 in the prior art, invention name: a novel processing method of ginger magnolia bark; the invention relates to a novel processing method of ginger magnolia officinalis, which comprises the following steps: pretreating raw materials, sealing and accumulating to induce sweating, mixing with rhizoma Zingiberis recens leaching solution after sweating, moistening, parching with slow fire, moistening, and standing at intervals to give cream. The magnolia bark processed by the traditional Chinese medicine processing method has high content of active ingredients, the surface of the magnolia bark has magnolia bark cream, and the magnolia bark cream with magnolia bark is superior to the magnolia bark of ginger in pharmacopoeia and the raw magnolia bark in anti-inflammatory and analgesic effects. This invention has the following problems: 1. the process focuses on the frost yield of the magnolia officinalis, and the content of magnolol and the content of active ingredients of honokiol are not determined; 2. the process is complicated.

The invention overcomes the technical problem of CN201310277299.0, and the total content of magnolol and magnolol is up to 3.34% by test; the ginger juice is uniformly stirred and baked, and the process is simple.

Prior art application No. 201910011757.3, title of invention: a preparation method of traditional Chinese medicine magnolia bark decoction pieces and a preparation method of ginger magnolia bark decoction pieces are provided, 1) coarse surface layers of magnolia bark raw materials are scraped to obtain crude magnolia bark; 2) drenching the crude cortex Magnolia officinalis, and moistening; 3) steaming the moistened crude cortex Magnolia officinalis in a steam box at 40-50 deg.C; 4) crushing the steamed magnolia officinalis, and drying to obtain magnolia officinalis decoction pieces; the drying process comprises a first drying section, a second drying section and a third drying section which are sequentially arranged, the temperatures of the first drying section, the second drying section and the third drying section are not higher than 80 ℃, the drying temperatures of the first drying section and the third drying section are both lower than the drying temperature of the second drying section, and the water content of the dried magnolia officinalis decoction pieces is not more than 10%; and the particle diameter of the crushed magnolia bark decoction pieces is 0.2-0.9 mm. The content of effective components is increased by improving the processing method, and the loss of the effective components is greatly reduced. This invention has the following problems: 1. it is not clear that the ginger juice and the magnolia bark decoction pieces are mixed, moistened and fried to brown temperature, so that the process is unstable, and the content of the magnolol and the effective components of the honokiol are not clear.

The invention overcomes the technical problem of application number 201910011757.3, and is obtained by uniformly stirring and baking ginger juice, and the process is simple and stable; tests show that the total content of the magnolia officinalis, the magnolol and the magnolol reaches 3.34 percent at most.

Application number in the prior art: 201610096047.1, title of the invention: the invention provides a processing method of ginger-processed magnolia officinalis, which comprises the following steps: cleaning cortex Magnolia officinalis, deactivating enzyme, inducing perspiration, preparing rhizoma Zingiberis recens juice, moistening, parching, inducing perspiration, and air drying. The processing method of the magnolia officinalis can improve the dissolution rate of the active ingredients of the magnolia officinalis, enhance the drug effect, and press the magnolia officinalis into a plate shape, thereby being convenient for cleaning and shredding, and being convenient for packaging and storage. However, this invention has the following problems: 1. the magnolia officinalis needs to be cleaned, de-enzymed and sweated, and the process is complex; 2. the ginger magnolia officinalis needs to be heated by slow fire and then by strong fire, the temperature is high, and active ingredients are easy to lose.

The invention overcomes the technical problem of application number 201610096047.1, and is obtained by uniformly stirring and baking the ginger juice, and the process is simple and stable; the active ingredients are not easy to lose, and the total content of the magnolia officinalis, the magnolol and the magnolol is up to 3.34 percent through test.

Application number CN201410574229.6 in the prior art, invention name: a process for preparing magnolia bark by ginger steaming method includes such steps as removing impurities, removing coarse bark, washing, moistening, shredding, baking in hot air circulating oven, mixing with ginger juice, steaming for 1-8 hr, taking out, baking and cooling. Compared with the classic ginger moxibustion method, the total amount of magnolol and honokiol in the product can reach 2.5%, the variation range is small, the production condition is easy to standardize, the problem of large environmental pollution caused by smoke dust in the production process of the ginger moxibustion method is solved, and the mangnolia officinalis prepared by the process has a good effect on the inhibition rate of swelling. However, this invention has the following problems: 1. the method needs to be performed with tight soaking, the shredded magnolia officinalis and the ginger juice are firstly steamed in a steam box and then dried in an oven, the time is long, and the cost is high; 2. the consumption of the ginger juice is not clear; 3. the content of magnolol and honokiol active ingredient is not clear.

The invention overcomes the technical problem of application number CN201410574229.6, the invention defines the using amount of ginger juice, the ginger juice is uniformly stirred and baked, and the process is simple and stable; the active ingredients are not easy to lose, and the total content of the magnolia officinalis, the magnolol and the magnolol is up to 3.34 percent through test.

The invention discloses a processing method of traditional Chinese medicine dried ginger, which comprises the steps of pretreatment, sweating, preparation of ginger and mangnolia officinalis extracting solution, stir-frying, preparation of processing liquid, moistening and frosting. In the processing method of the dried ginger, the bee pollen contains a plurality of trace elements and a large amount of active substances such as active protease, nucleic acid, flavonoid compounds and the like, the dissolution rate and the drug effect of the active ingredients are enhanced after the dried ginger is moistened by the processing liquid of the bee pollen, the final cream-forming method can be used for enhancing the anti-inflammatory effect of the dried ginger, the cream-forming rate is higher, and the anti-inflammatory and analgesic effects are obviously improved. However, this invention has the following problems: 1. the ginger needs to be sweated, the magnolia officinalis needs to be extracted by heat for 2-3 times, the process is complex, the time is long, and the cost is high; 2. the content of magnolol and honokiol active ingredient is not clear.

The invention overcomes the technical problem of application number 201710220974.4, and is obtained by uniformly stirring and baking the ginger juice, and the process is simple and stable; the active ingredients are not easy to lose, and the total content of the magnolia officinalis, the magnolol and the magnolol is up to 3.34 percent through test.

Application number in the prior art: 201810912870.4, title of the invention: the invention discloses a processing method of magnolia officinalis, which comprises the following steps: 1) selecting cortex Magnolia officinalis, cutting into shreds, adding vinegar into rhizoma Zingiberis recens juice, stirring, soaking, and boiling, wherein the weight of the vinegar added into rhizoma Zingiberis recens juice is 10-15% of that of cortex Magnolia officinalis shreds; 2) taking out and airing to obtain the product. The invention can keep the effective components of the magnolia officinalis by adding the ginger juice and the vinegar. However, this invention has the following problems: 1. the ginger juice is not boiled or squeezed definitely; 2. the content of magnolol and honokiol active ingredient is not clear.

The invention overcomes the problem of application number 201810912870.4, clearly shows that the ginger juice has better effect through tests, and tests show that the total content of magnolia officinalis, magnolol and magnolol reaches up to 3.34 percent.

In conclusion, the invention researches and develops a magnolia officinalis processed product and a processing and detecting method in order to solve the technical problems of complex process, low total content of honokiol and magnolol in magnolia officinalis, unscientific detecting method and the like in the prior art.

Disclosure of Invention

The invention aims to provide a magnolia officinalis processed product;

the other purpose of the invention is to provide a processing method of the magnolia officinalis processed product;

the invention also aims to provide a detection method of the magnolia officinalis processed product.

The processed product comprises the following components in percentage by weight: the ginger juice is 2: 1-18: 1.

Preferably, the processed product comprises the following components in percentage by weight: the ginger juice is 6: 1-14: 1.

Further preferably, the processed product comprises the following components in percentage by weight: the ginger juice is 8: 1-12: 1.

Further preferably, the processed product comprises the following components in percentage by weight: the ratio of ginger juice to ginger juice is 10: 1.

The processing method of the magnolia officinalis processed product comprises the following steps:

1) cleaning ginger, mashing, adding 1-5 times of water, squeezing to obtain juice, adding 1-4 times of water into ginger residue, repeatedly squeezing once, and mixing the juice to obtain ginger juice;

2) shredding cortex magnoliae officinalis to obtain shredded cortex magnoliae officinalis, adding ginger juice, stirring uniformly, placing in an oven, drying at 60-90 ℃ for 2-5 h, taking out, and air drying to obtain the final product.

Preferably, the processing method of the magnolia officinalis processed product comprises the following steps:

1) cleaning ginger, mashing, adding 2-4 times of water, squeezing to obtain juice, adding 2-3 times of water into ginger residue, repeatedly squeezing once, and mixing the juice to obtain ginger juice;

2) shredding cortex magnoliae officinalis to obtain shredded cortex magnoliae officinalis, adding ginger juice, stirring uniformly, placing in an oven, drying at 65-85 ℃ for 3-4 h, taking out, and air drying to obtain the final product.

Further preferably, the processing method of the magnolia officinalis processed product comprises the following steps:

1) cleaning rhizoma Zingiberis recens, mashing, adding 3 times of water, squeezing to obtain juice, adding 2 times of water into rhizoma Zingiberis recens residue, squeezing once again, and mixing the juices to obtain rhizoma Zingiberis recens juice;

2) slicing cortex Magnolia officinalis to obtain sliced cortex Magnolia officinalis, adding succus Zingiberis recens, stirring, oven drying at 80 deg.C for 3 hr, taking out, and air drying.

The detection method of the magnolia officinalis processed product comprises the following steps:

1) chromatographic conditions and System suitability test

Octadecylsilane chemically bonded silica is used as a filling agent; methanol-water with a mobile phase ratio of 65-85: 15-28; detection wavelength: 275-300 nm, a column temperature of 26-33 ℃, a flow rate of 1mL/min, a theoretical plate number of not less than 8000 according to magnolol and honokiol peaks, and a chromatographic column of 250mm multiplied by 4.6mm of an S/N4DG70548 column;

2) preparation of control solutions

Accurately weighing appropriate amount of honokiol and magnolol reference substance, respectively, adding methanol to dissolve and dilute to scale, making into solution containing magnolol 40 μ g and magnolol 24 μ g per 1mL, and filtering with 0.45 μm microporous membrane to obtain reference substance solution;

3) preparation of test solution

Accurately weighing 0.2g of magnolia officinalis powder, adding 20-30 ml of analysis methanol into a conical flask with a plug of 150ml, shaking up, sealing the conical flask, quantitatively weighing, carrying out ultrasonic extraction for 20-50 min, quantitatively weighing again, supplementing the loss weight with the analysis methanol, filtering, accurately weighing 5ml of subsequent filtrate, placing the subsequent filtrate in a volumetric flask of 25ml, adding methanol to scale, shaking up, fixing the volume, and filtering the filtrate through a microporous filter membrane of 0.45 mu m to obtain a sample solution.

Preferably, the detection method of the magnolia officinalis processed product comprises the following steps:

1) chromatographic conditions and System suitability test

Octadecylsilane chemically bonded silica is used as a filling agent; methanol-water with a mobile phase ratio of 70-82: 18-25; detection wavelength: 280-298 nm, a column temperature of 28-32 ℃, a flow rate of 1mL/min, a theoretical plate number of which is not less than 8000 according to magnolol and honokiol peaks, and a chromatographic column of 250mm multiplied by 4.6mm of an S/N4DG70548 column;

2) preparation of control solutions

3) preparation of test solution

Accurately weighing 0.2g of magnolia officinalis powder, adding 20-30 ml of analysis methanol into a conical flask with a plug of 150ml, shaking up, sealing the conical flask, quantitatively weighing, carrying out ultrasonic extraction for 25-40 min, quantitatively weighing again, supplementing the loss weight with the analysis methanol, filtering, accurately weighing 5ml of subsequent filtrate, placing the subsequent filtrate in a volumetric flask of 25ml, adding methanol to scale, shaking up, fixing the volume, and filtering the filtrate through a microporous filter membrane of 0.45 mu m to obtain a sample solution.

Further preferably, the detection method of the magnolia officinalis processed product comprises the following steps:

1) chromatographic conditions and System suitability test

Octadecylsilane chemically bonded silica is used as a filling agent; methanol-water with a mobile phase ratio of 78: 22; detection wavelength: 294nm, column temperature of 30 ℃, flow rate of 1m L/min, theoretical plate number not less than 8000 according to magnolol and honokiol peak, chromatographic column of 250mm × 4.6mm of S/N4DG70548 column;

2) preparation of control solutions

3) preparation of test solution

Precisely weighing 0.2g of magnolia officinalis powder, adding 25ml of analysis methanol into a conical flask with a plug, shaking uniformly, sealing the conical flask, quantitatively weighing, carrying out ultrasonic extraction for 30min, further quantitatively weighing, supplementing the loss weight with the analysis methanol, filtering, precisely weighing 5ml of subsequent filtrate, placing the subsequent filtrate in a 25ml volumetric flask, adding methanol to the scale, shaking uniformly, fixing the volume, and filtering the filtrate through a 0.45 mu m microporous filter membrane to obtain a sample solution.

The ratio of the ginger juice to the ginger is 1: 1, i.e. 1g of ginger, 1ml of ginger juice needs to be squeezed out.

The density of the ginger juice is 1 g/ml.

Has the advantages that:

1. the invention optimizes the processed products of the ginger processed magnolia bark and the processing method thereof through tests, and defines the best method of the ginger processed magnolia bark, and the total content of honokiol and magnolol reaches 3.34 percent by using the method.

2. The invention takes magnolia officinalis processed by four processing methods of ginger juice boiling, ginger juice frying, ginger juice boiling and ginger juice frying respectively for test, and the data can be known through visual analysis: the processing can reduce the content of phenolic components in the magnolia officinalis; the content of the ginger juice stir-fried in the four processing methods of the magnolia officinalis is obviously higher than that of other processing methods, and the method has statistical significance compared with other processing methods; therefore, the best processing method is the mixing and frying of the ginger juice in different processing methods.

3. The variance analysis is carried out on the measurement result, and the analysis by SPSS software shows that: the content determination results of magnolol and honokiol in magnolia officinalis with different auxiliary material proportions have significant differences. The content measurement results of the ginger-processed magnolia officinalis with different auxiliary material ratios show that the total content of the active ingredients of the processed product of the magnolia officinalis and the squeezed ginger juice is high in a ratio of 10:1, so that 10:1 is selected as the optimal auxiliary material ratio.

4. Through the positive cross validation test, the highest value of the results in the orthogonal test table can be achieved, and the optimal processing process condition selected by the orthogonal test is stable.

5. The invention shows that the magnolol is in a linear relation of 2-20 mu l and the honokiol is in a linear relation of 1-15 mu l through linear relation examination.

6. According to the invention, the precision tests show that the RSD of the peak areas of the magnolol and the honokiol obtained by calculation are 1.62% and 0.16% respectively, which indicates that the precision of the instrument is better.

7. The invention shows through repeatability tests that the RSD of the calculated magnolol and honokiol content is 0.6124% and 0.7019% respectively, which shows that the method has good repeatability.

8. The stability test shows that the test solution is in a stable state within 12 hours.

9. The invention shows that the average sample recovery rate of magnolol is 97.82 percent respectively through sample recovery test,

99.78%, 98.84%, in the range specified by 95% to 105%, indicating that the accuracy of the determination method is high; the average sample adding recovery rate of honokiol is respectively 98.81%, 97.90% and 95.05%, and is within the specified range of 95% -105%, which indicates that the accuracy of the determination method is high.

10. The invention discloses a processing method of traditional Chinese medicine magnolia officinalis with ginger under the name of application number CN200910095729.0 in the prior art, and has the following problems: 1. the mangnolia officinalis is cut into 1cm long shreds after being softened at normal temperature, and the shreds are placed in an oven at 50-60 ℃ for drying for 1-1.5 hours, so that the effective components of the mangnolia officinalis are easily lost due to direct drying in the oven; 2. the stacking thickness during drying is not clear, and the time is not easy to control; 3. the microwave processing is carried out for 15-30 minutes under the power of 400-700W, and the active ingredients of the mangnolia officinalis processed by the microwave are likely to be lost; the specification shows that the sum of the contents of magnolol and honokiol in the processed product of the traditional Chinese medicine magnolia officinalis is about 2.91 percent, and the effective components are low.

11. The invention discloses a novel processing method of magnolia officinalis with ginger under the name of application number CN201610096597.3 in the prior art, and has the following problems: 1. the squeezed ginger juice is heated and boiled after being subjected to ultrasonic treatment, and the treatment process of the ginger juice is complicated; 2. the ginger juice is added into the magnolia officinalis, the heating and moistening are carried out for 6 hours, the sweating is carried out, the processing time is long, the cost is high, and the effective components are easy to lose due to the fact that the heating temperature is not clear; 3. the feasibility of the process was not confirmed by orthogonal experiments.

12. Application number CN201310277299.0 in the prior art, invention name: a novel processing method of ginger magnolia bark; there are the following problems: 1. the process focuses on the frost yield of the magnolia officinalis, and the content of magnolol and the content of active ingredients of honokiol are not determined; 2. the process is complicated.

13. Prior art application No. 201910011757.3, title of invention: the preparation method of the traditional Chinese medicine magnolia bark decoction pieces and the preparation method of the ginger magnolia bark decoction pieces have the following problems: 1. it is not clear that the ginger juice and the magnolia bark decoction pieces are mixed, moistened and fried to brown temperature, so that the process is unstable, and the content of the magnolol and the effective components of the honokiol are not clear.

14. Application number in the prior art: 201610096047.1, title of the invention: a processing method of magnolia officinalis with ginger has the following problems: 1. the magnolia officinalis needs to be cleaned, de-enzymed and sweated, and the process is complex; 2. the ginger magnolia officinalis needs to be heated by slow fire and then by strong fire, the temperature is high, and active ingredients are easy to lose.

15. Application number CN201410574229.6 in the prior art, invention name: a processing technology of magnolia officinalis by ginger steaming has the following problems: 1. the method needs to be performed with tight soaking, the shredded magnolia officinalis and the ginger juice are firstly steamed in a steam box and then dried in an oven, the time is long, and the cost is high; 2. the consumption of the ginger juice is not clear; 3. the content of magnolol and honokiol active ingredient is not clear.

16. The invention discloses a processing method of traditional Chinese medicine dried ginger, which is applied under the name of 201710220974.4 in the prior art and has the following problems: 1. the ginger needs to be sweated, the magnolia officinalis needs to be extracted by heat for 2-3 times, the process is complex, the time is long, and the cost is high; 2. the content of magnolol and honokiol active ingredient is not clear.

17. Application number in the prior art: 201810912870.4, title of the invention: a processing method of magnolia officinalis has the following problems: 1. the ginger juice is not boiled or squeezed definitely; 2. the content of magnolol and honokiol active ingredient is not clear.

Detailed Description

The technical solution of the present invention will be further specifically described below by way of specific examples.

Example 1 processed product of Magnolia officinalis and method for preparing the same

The components: 100kg of mangnolia officinalis and 10kg of ginger juice.

The preparation method comprises the following steps:

Example 2 processed product of Magnolia officinalis and method for preparing the same

The components: 100kg of mangnolia officinalis and 10kg of ginger juice.

The preparation method comprises the following steps:

1) cleaning rhizoma Zingiberis recens, mashing, adding 2 times of water, squeezing to obtain juice, adding 2 times of water into rhizoma Zingiberis recens residue, repeatedly squeezing once, and mixing the juices to obtain rhizoma Zingiberis recens juice;

2) shredding cortex Magnolia officinalis to obtain shredded cortex Magnolia officinalis, adding succus Zingiberis recens, stirring, oven drying at 65 deg.C for 3 hr, taking out, and air drying.

Example 3 processed product of Magnolia officinalis and method for preparing the same

The components: 100kg of mangnolia officinalis and 10kg of ginger juice.

The preparation method comprises the following steps:

1) cleaning rhizoma Zingiberis recens, mashing, adding 4 times of water, squeezing to obtain juice, adding 3 times of water into rhizoma Zingiberis recens residue, repeatedly squeezing once, and mixing the juices to obtain rhizoma Zingiberis recens juice;

2) slicing cortex Magnolia officinalis to obtain sliced cortex Magnolia officinalis, adding succus Zingiberis recens, stirring, oven drying at 85 deg.C for 4 hr, taking out, and air drying.

Example 4 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 10kg of ginger juice.

The preparation method comprises the following steps:

1) cleaning rhizoma Zingiberis recens, mashing, adding 1 times of water, squeezing to obtain juice, adding 1 times of water into rhizoma Zingiberis recens residue, squeezing once again, and mixing the juices to obtain rhizoma Zingiberis recens juice;

2) shredding cortex Magnolia officinalis to obtain shredded cortex Magnolia officinalis, adding succus Zingiberis recens, stirring, oven drying at 60 deg.C for 2 hr, taking out, and air drying.

Example 5 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 10kg of ginger juice.

The preparation method comprises the following steps:

1) cleaning rhizoma Zingiberis recens, mashing, adding 5 times of water, squeezing to obtain juice, adding 4 times of water into rhizoma Zingiberis recens residue, repeatedly squeezing once, and mixing the juices to obtain rhizoma Zingiberis recens juice;

2) slicing cortex Magnolia officinalis to obtain sliced cortex Magnolia officinalis, adding succus Zingiberis recens, stirring, oven drying at 90 deg.C for 5 hr, taking out, and air drying.

Example 6 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 50kg of ginger juice.

The preparation method comprises the following steps:

Example 7 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 50kg of ginger juice.

The preparation method comprises the following steps:

Example 8 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 50kg of ginger juice.

The preparation method comprises the following steps:

Example 9 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 50kg of ginger juice.

The preparation method comprises the following steps:

Example 10 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 50kg of ginger juice.

The preparation method comprises the following steps:

Example 11 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 16.7kg of ginger juice.

The preparation method comprises the following steps:

Example 12 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 16.7kg of ginger juice.

The preparation method comprises the following steps:

Example 13 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 16.7kg of ginger juice.

The preparation method comprises the following steps:

Example 14 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 16.7kg of ginger juice.

The preparation method comprises the following steps:

Example 15 processed product of Magnolia officinalis and method of preparation

The components: 100kg of mangnolia officinalis and 16.7kg of ginger juice.

The preparation method comprises the following steps:

Example 16 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 7.1kg of ginger juice.

The preparation method comprises the following steps:

Example 17 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 7.1kg of ginger juice.

The preparation method comprises the following steps:

Example 18 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 7.1kg of ginger juice.

The preparation method comprises the following steps:

Example 19 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 7.1kg of ginger juice.

The preparation method comprises the following steps:

Example 20 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 7.1kg of ginger juice.

The preparation method comprises the following steps:

Example 21 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 5.6kg of ginger juice.

The preparation method comprises the following steps:

Example 22 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 5.6kg of ginger juice.

The preparation method comprises the following steps:

Example 23 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 5.6kg of ginger juice.

The preparation method comprises the following steps:

Example 24 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 5.6kg of ginger juice.

The preparation method comprises the following steps:

Example 25 processed product of Magnolia officinalis and method of preparation

The components: 100kg of magnolia officinalis and 5.6kg of ginger juice.

The preparation method comprises the following steps:

Example 26 processed product of Magnolia officinalis and method of preparation

The components: 60kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

Example 27 processed product of Magnolia officinalis and method of preparation

The components: 60kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

Example 28 processed product of Magnolia officinalis and method of preparation

The components: 60kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

Example 29 processed product of Magnolia officinalis and method of preparation

The components: 60kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

Example 30 processed product of Magnolia officinalis and method of preparation

The components: 60kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

Example 31 processed product of Magnolia officinalis and method of preparation

The components: 14kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

Example 32 processed product of Magnolia officinalis and method of preparation

The components: 14kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

Example 33 processed product of Magnolia officinalis and method of preparation

The components: 14kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

Example 34 processed product of Magnolia officinalis and method of preparation

The components: 14kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

Example 35 processed product of Magnolia officinalis and method of preparation

The components: 14kg of mangnolia officinalis and 1kg of ginger juice.

The preparation method comprises the following steps:

The processed articles of examples 1 to 35 were examined by the examination method of any of examples 36 to 40

Example 36 detection method

1) Chromatographic conditions and System suitability test

Octadecylsilane chemically bonded silica is used as a filling agent; methanol-water with a mobile phase ratio of 78: 22; detection wavelength: 294nm, column temperature of 30 ℃, flow rate of 1mL/min, theoretical plate number not less than 8000 according to magnolol and honokiol peak, chromatographic column of 250mm × 4.6mm of S/N4DG70548 column;

2) preparation of control solutions

3) preparation of test solution

Example 37 detection method

1) Chromatographic conditions and System suitability test

Octadecylsilane chemically bonded silica is used as a filling agent; methanol-water with a mobile phase ratio of 72: 28; detection wavelength: 275nm, column temperature of 26 ℃, flow rate of 1mL/min, theoretical plate number not less than 8000 according to magnolol and honokiol peak, chromatographic column of 250mm × 4.6mm of S/N4DG70548 column;

2) preparation of control solutions

3) preparation of test solution

Accurately weighing 0.2g of Magnolia officinalis powder, adding 20ml of analytical methanol into 150ml of conical flask with plug, shaking, sealing, quantitatively weighing, ultrasonically extracting for 20min, quantitatively weighing, supplementing the loss weight with analytical methanol, filtering, accurately weighing 5ml of subsequent filtrate, placing in 25ml volumetric flask, adding methanol to scale, shaking, and fixing volume, and filtering the filtrate with 0.45 μm microporous membrane to obtain sample solution.

Example 38 detection method

1) Chromatographic conditions and System suitability test

Octadecylsilane chemically bonded silica is used as a filling agent; methanol-water with a mobile phase ratio of 85: 15; detection wavelength: 300nm, a column temperature of 33 ℃, a flow rate of 1mL/min, a theoretical plate number of which is not less than 8000 according to magnolol and honokiol peaks, and a chromatographic column of 250mm multiplied by 4.6mm of an S/N4DG70548 column;

2) preparation of control solutions

3) preparation of test solution

Accurately weighing 0.2g of Magnolia officinalis powder, adding 30ml of analytical methanol into 150ml of conical flask with plug, shaking, sealing, quantitatively weighing, ultrasonically extracting for 50min, quantitatively weighing, supplementing the loss weight with analytical methanol, filtering, accurately weighing 5ml of subsequent filtrate, placing in 25ml volumetric flask, adding methanol to scale, shaking, and metering volume, filtering the filtrate with 0.45 μm microporous membrane to obtain sample solution.

Example 39 detection method

1) Chromatographic conditions and System suitability test

Octadecylsilane chemically bonded silica is used as a filling agent; methanol-water with a mobile phase ratio of 75: 25; detection wavelength: 280nm, a column temperature of 28 ℃, a flow rate of 1mL/min, a theoretical plate number of which is not less than 8000 according to magnolol and honokiol peaks, and a chromatographic column of 250mm multiplied by 4.6mm of an S/N4DG70548 column;

2) preparation of control solutions

3) preparation of test solution

Accurately weighing 0.2g of Magnolia officinalis powder, adding 20ml of analytical methanol into 150ml of conical flask with plug, shaking, sealing, quantitatively weighing, ultrasonically extracting for 25min, quantitatively weighing, supplementing the loss weight with analytical methanol, filtering, accurately weighing 5ml of subsequent filtrate, placing in 25ml volumetric flask, adding methanol to scale, shaking, and metering volume, and filtering the filtrate with 0.45 μm microporous membrane to obtain sample solution.

Example 40 detection method

1) Chromatographic conditions and System suitability test

Octadecylsilane chemically bonded silica is used as a filling agent; methanol-water with a mobile phase ratio of 82: 18; detection wavelength: 298nm, column temperature 32 deg.C, flow rate 1mL/min, theoretical plate number not less than 8000 according to magnolol and honokiol peak, and S/N4DG70548 column 250mm × 4.6 mm;

2) preparation of control solutions

3) preparation of test solution

Accurately weighing 0.2g of Magnolia officinalis powder, adding 30ml of analytical methanol into 150ml of conical flask with plug, shaking, sealing, quantitatively weighing, ultrasonically extracting for 40min, quantitatively weighing, supplementing the loss weight with analytical methanol, filtering, accurately weighing 5ml of subsequent filtrate, placing in 25ml volumetric flask, adding methanol to scale, shaking, and fixing volume, and filtering the filtrate with 0.45 μm microporous membrane to obtain sample solution.

The team of the invention screens the best scheme of the invention and confirms the beneficial effects thereof through the following tests, in particular as follows:

1 Instrument and reagent

1.1 instruments

Agilent 1260 high performance liquid chromatograph; agilent 5TC-C18(2) column 250X 4.6 mm; (ii) a Ten-thousandth electronic balance (ME204E/02 from Shanghai Metler-Toliduo instruments, Inc.), one hundred thousand analytical balance (AUW220D from Suzhou Shimadzu instruments, Inc.), portable high-speed universal pulverizer (Lingdan brand LDP-100 from Red Sun electromechanical, Inc. of Yongkang, Zhejiang), ultrasonic cleaner (Shanghai Yijing ultrasonic instruments, Inc.); circulating water type vacuum pump (model: SHZ-95, Yingyu Zhihua instruments ltd, Henan, China) electrothermal blowing dry box (model: 101-2AB, Taster instruments ltd, Tianjin) digital display constant temperature water bath (model: HH2, Australia instruments ltd, Hezhou) juice extractor (model: Jiuyang JYL-C91T, produced by Jiuyang Gmby)

1.2 reagent

Honokiol reference substances (batch number GZDD-0035, Guizhou Di Biotechnology, Inc.), magnolol reference substances (batch number GZDD-0070, Guizhou Di Biotechnology, Inc.), chromatographic methanol from Tianjin Kemiou chemical reagent, Inc., ultrapure water as Wahaha purified water, and the rest of the reagents are analytically pure.

2 sources of samples

The purchasing place of the magnolia officinalis is Meitan county Jinpancun in Zunyi City. Altitude 1104 m, Beijing 27 ° 35 '8 ", Tokyo 107 ° 26' 3". The collecting time is 2016 years, 5 months and 21 times, and the collected magnolia officinalis medicinal materials mainly comprise dry bark, root bark and branch bark. Ginger is purchased in the farmer market in the Huaxi region of Guiyang city.

3 processing of ginger magnolia bark

3.1 preparation of ginger juice

3.1.1 preparation of the boiled ginger juice: taking 300g of ginger, adding three times of water, placing in a stainless steel pot, decocting with slow fire, filtering with gauze to obtain 300ml of ginger juice for later use

3.1.2 preparation of squeezed ginger juice: 300g of ginger is taken, water with equal amount is added, juice is squeezed by a juice extractor, and then the juice is filtered by gauze, so that 300ml of squeezed ginger juice is prepared for standby.

3.2 preparation of ginger-processed Magnolia bark

Mixing shredded cortex Magnolia officinalis with prepared succus Zingiberis recens, moistening for 2 hr, oven drying to near dry, and cooling.

3.2.1 proportions of different adjuvants

Taking about 100g of five parts of shredded magnolia officinalis, sampling the shredded magnolia officinalis and the squeezed ginger juice according to the proportion of 2:1, 6:1, 10:1, 14:1 and 18:1, diluting the ginger juice with a proper amount of water, uniformly mixing the diluted ginger juice with the magnolia officinalis, moistening the mixture and cooling the mixture. Pulverizing and sieving with a third sieve. Bagging for later use. The specific process is shown in table 1 below.

TABLE 1 preparation of ginger-magnolia bark with different adjuvant proportions

3.2.2 different baking temperatures

Taking about 100g of five parts of shredded magnolia officinalis, diluting 10ml of prepared ginger juice with 20ml of water, uniformly stirring, moistening, putting into an oven, drying at 50 ℃, 60 ℃, 70 ℃, 80 ℃ and 90 ℃, cooling, pulverizing, and sieving with a third sieve. Bagging for later use. The specific process is shown in table 2 below.

TABLE 2 preparation of ginger-processed magnolia bark with different baking temperatures

3.2.3 different baking times

Taking 100g of five parts of shredded magnolia officinalis, diluting with 10ml of prepared ginger juice and 20ml of water respectively, stirring uniformly, moistening, and placing into an oven to bake at 70 ℃ for 180min, 210min, 240min, 270min and 300 min. Cooling, pulverizing, sieving with a third sieve, and bagging. The specific procedure is shown in table 3 below.

TABLE 3 preparation of cortex Magnolia officinalis with different baking times

3.2.4 different moistening time

Taking about 100g of six parts of shredded magnolia officinalis, diluting with 10ml of prepared ginger juice and 20ml of water respectively, uniformly stirring, moistening for 3h, 6h, 9h, 12h, 15h and 18h respectively, and putting into an oven to bake for 4h at 60 ℃. Cooling, pulverizing, and sieving with a third sieve. Bagging for later use. The specific procedure is shown in table 4 below.

TABLE 4 preparation of Magnolia officinalis with different moistening time

4.1 methodological investigation

4.1.1 chromatographic conditions and System suitability test

Octadecylsilane chemically bonded silica is used as a filling agent; the mobile phase is methanol-water (78: 22); detection wavelength: 294nm, column temperature 30 ℃ and flow rate 1 mL/min. The theoretical plate number is not less than 8000 according to magnolol and honokiol peak. Column for chromatography, S/N4DG70548 column 250mm × 4.6 mm.

4.1.2 preparation of control solutions

Accurately weighing appropriate amount of honokiol and magnolol reference substance, respectively, adding methanol to dissolve and dilute to scale, making into solution containing magnolol 40 μ g and magnolol 24 μ g per 1mL, and filtering with 0.45 μm microporous membrane to obtain reference substance solution.

4.1.3 preparation of test solutions

Taking about 0.2g of cortex Magnolia officinalis powder (passing through a third sieve), precisely weighing (one ten-thousandth of the powder), adding into 150ml conical flask with plug, adding 25ml of analytical methanol, shaking, sealing the plug, quantitatively weighing, and ultrasonically extracting for 30 min. Quantitatively weighing again, supplementing the lost weight with analytical methanol, filtering, precisely measuring the subsequent filtrate 5ml, placing in a 25ml volumetric flask, adding methanol to scale, shaking up, and fixing the volume. Filtering the filtrate with 0.45 μm microporous membrane to obtain test solution.

4.1.4 examination of the Linear relationship

Precisely weighing the honokiol and the honokiol respectively at 10.32mg and 10.14mg, putting the honokiol and the honokiol into a 25ml volumetric flask, adding a proper amount of methanol to dissolve the honokiol and diluting the honokiol to the scale to obtain stock solutions with the concentrations of 412.28 mu g/ml and 405.6 mu g/ml respectively.

Taking the stock solution, precisely diluting to obtain a magnolol control solution with a magnolol concentration of 49.54 mu g/ml and a honokiol concentration of 10.14 mu g/ml, performing injection measurement on magnolol with injection volumes of 2 mu l, 5 mu l, 10 mu l, 15 mu l, 20 mu l and honokiol of 1ul, 2 mu l, 5 mu l, 10 mu l and 15 mu l according to chromatographic conditions of 4.1.1, performing regression treatment by taking the injection mass (X) of the control as an abscissa and the peak area (Y) as an ordinate to obtain a magnolol regression equation of Y-83.791X +7.7488 (r-0.9999) and a honokiol regression equation of Y-30.613X +0.1363(0.9999), wherein the magnolol regression equation shows that the honokiol is in a range of 2 mu l-20 mu l and the honokiol has a good linear relationship in a range of 1 mu l-15 mu l, and the result is shown in a table of 5 and 6.

TABLE 5 examination of magnolol Linear relationship

TABLE 6 examination of the Honokiol Linear relationship

4.1.5 precision test

Precisely sucking a reference substance solution under item 4.1.2, filtering the reference substance solution through a 0.45-micrometer microporous filter membrane, accurately injecting 5 mu L of the reference substance solution under the chromatographic condition under item 4.1.1, continuously injecting for 6 times, respectively recording peak areas, and respectively calculating to obtain the RSD of the peak areas of magnolol and honokiol to be 1.62% and 0.16%, which indicates that the precision of the instrument is better, and the result is shown in Table 7.

TABLE 7 results of precision test

4.1.6 repeatability test

Taking 6 parts of magnolia officinalis sample, each part is about 0.2g, precisely weighing (accurate to one ten thousand), preparing a test solution according to the method under 4.1.3, respectively carrying out sample injection measurement according to the chromatographic conditions under 4.1.1, and calculating the RSD of magnolol and honokiol content to be 0.6124% and 0.7019%, wherein the result shows that the method has good repeatability and the result is shown in Table 8.

TABLE 8 results of the repeatability tests

4.1.7 stability test

Precisely measuring the same test solution, accurately injecting 5 μ L sample at 0h, 2h, 4h, 6h, 8h, 10h and 12h, recording peak area, and calculating the RSD value of magnolol and honokiol peak area, the result is shown in Table 9.

TABLE 9 stability test results

As can be seen from the above table, the average value of the magnolol peak area of the test sample is 879.31, RSD is 0.17%, the average value of the honokiol peak area is 100.24, and RSD is 0.66%. The results show that the test solution was in a steady state within 12 hours.

4.1.8 sample recovery test

Weighing 9 parts of magnolia officinalis powder sample, wherein each part is about 0.1g, precisely weighing (accurate to one ten thousand) 80%, 100% and 120% of the magnolia officinalis powder sample, respectively, adding a honokiol reference substance and a honokiol reference substance according to 3 levels, preparing a test solution according to a preparation method of the test solution under the '4.1.3' method, measuring according to a content measurement method, and respectively calculating the recovery rate, wherein the results are shown in a table 10 and a table 11.

Recovery rate/% (actual measurement-amount of the component to be measured contained in the test sample)/amount of the control added × 100%.

TABLE 10 magnolol sample recovery test results

As can be seen from the table, the average sample recovery rate of magnolol is 97.82%, 99.78%, and 98.84%, respectively, and is within the specified range of 95% to 105%, indicating that the accuracy of the determination method is high.

TABLE 11 sample recovery measurement results for honokiol

As can be seen from the table, the average recovery rates of honokiol added to the sample are respectively 98.81%, 97.90% and 95.05%, and are within the specified ranges of 95% to 105%, indicating that the accuracy of the measurement method is high.

4.1.9 assay of samples

Taking about 0.2g of cortex magnoliae officinalis sample powder, precisely weighing, preparing a test solution according to the preparation method under item 4.1.3, precisely absorbing 5 mu L of the test solution respectively, injecting the test solution into a high performance liquid chromatograph, recording peak areas, calculating the contents of magnolol and honokiol of cortex magnoliae officinalis, and calculating the result according to the dry product, wherein the result is shown in table 12.

TABLE 12 determination of Magnolia cortex with ginger (n ═ 3)

4.2 examination of extraction conditions

4.2.1 examination of extraction methods

Taking about 0.2g of Magnolia officinalis powder, precisely weighing, adding into 150ml conical flask with plug, adding 25ml of analytical methanol, shaking, sealing, ultrasonic extracting, and cold soaking respectively. Ultrasonic extracting for 30min by ultrasonic method, and cold soaking for 24h by cold soaking method. Filtering, precisely measuring 5ml of the subsequent filtrate, placing in a 25ml measuring flask, adding methanol to scale, shaking, and filtering the filtrate with 0.45 μm microporous membrane. The results are shown in Table 13.

TABLE 13 examination of the extraction methods

The analysis of the measured result shows that under the same condition, the total content of magnolol and honokiol measured by an ultrasonic extraction method is larger than that measured by a cold-soaking extraction method. Because the ultrasonic extraction method can save time, the ultrasonic method is selected as the extraction method.

4.2.2 examination of extraction time

Precisely weighing about 0.2g of cortex Magnolia officinalis powder, adding into 150ml conical flask with plug, adding 25ml of analytical methanol, shaking, sealing, quantitatively weighing, and ultrasonic extracting for 20, 30, 40, and 50min respectively. Weighing again, supplementing the loss weight with analytical methanol, filtering, precisely measuring to obtain 5ml of the subsequent filtrate, placing in a 25ml measuring flask, adding methanol to scale, shaking, and filtering the filtrate with 0.45 μm microporous membrane. The results are shown in Table 14.

TABLE 14 examination of extraction times

And (3) analyzing the variance of the measurement result, and analyzing by SPSS software, wherein the different ultrasonic extraction times have significant difference on the content measurement result of magnolol and honokiol in the magnolia officinalis. As can be seen from Table 10 above, the total content of magnolol and honokiol measured at 30min and 40min is higher, and the total content value is almost the same, so that 30min with less time consumption is selected as the optimal extraction time.

4.3 examination of Single factor

4.3.1 examination of the proportions of the different adjuvants

Taking about 0.2g of Magnolia officinalis powder with different adjuvant ratios under item 3.2.1, preparing test solution according to item 4.1.3, and determining the results shown in Table 16.

TABLE 16 examination of the proportions of various adjuvants

The variance analysis is carried out on the measurement result, and the analysis by SPSS software shows that: the content determination results of magnolol and honokiol in magnolia officinalis with different auxiliary material proportions have significant differences. The content determination results of the ginger-processed magnolia officinalis with different auxiliary material proportions show that the magnolia officinalis and the squeezed ginger juice are sampled according to the proportion of 10:1, are moistened, and the total content of the effective components of the baked processed product is higher than the total content of the effective components of the processed product according to the proportion of 2:1, 6:1, 14:1 and 18: 1. Therefore, the optimal proportion of the auxiliary materials is 10: 1.

4.3.3 investigation of different baking temperatures

Taking about 0.2g of Magnolia officinalis powder with different adjuvant ratios under item 3.2.2, preparing test solution according to the method under item 4.1.3, and determining the results shown in Table 17.

TABLE 17 determination of the contents of different baking temperatures

Analysis of variance was performed on the measurements of table 13, and analysis by SPSS software showed: the content determination results of magnolol and honokiol in the cortex magnoliae officinalis with different baking temperatures have significant difference. The measurement results of the content of the magnolia officinalis in the ginger processed foods at different baking temperatures show that: when the baking temperature is 70 ℃, the content of magnolol and honokiol is the highest, so that the optimal baking temperature is determined to be 70 ℃.

4.3.4 examination of different baking times

Taking about 0.2g of Magnolia officinalis powder with different baking time under item 3.2.3, preparing test solution according to the test preparation method under item 4.1.3, and determining the results are shown in Table 18.

TABLE 18 determination of the content at different baking times

Variance analysis is carried out on the measurement results of different baking times, and SPSS analysis shows that: the content determination results of magnolol and honokiol in the cortex magnoliae officinalis with ginger in different baking time are obviously different. The measurement results of the content of the magnolia officinalis after baking for different baking times show that: when the baking time is 300min, the content of the effective components is the highest, so that 300min is determined as the optimal baking time. 4.3.5 investigation of different smoldering times

About 0.2g of Magnolia officinalis powder with different moistening time under item 3.2.4 was used to prepare test solutions according to the method under item 4.1.3, and the test results are shown in Table 19.

TABLE 19 measurement of content at different smoldering times

Variance analysis is carried out on the measurement results of different moistening times, and SPSS analysis shows that: the content determination results of magnolol and honokiol in the cortex magnoliae officinalis have significant difference in different moistening time. The content determination results of the magnolia officinalis in different moistening times show that: the total content of effective components of cortex Magnolia officinalis is the highest after moistening for 6 hr. Therefore, the optimal moistening time is 6 hours.

4.4 orthogonal Experimental investigation

4.4.1 determination of factor level

And determining the optimal conditions of different processing technologies of the magnolia officinalis from the result of single factor investigation. Adopting comprehensive scoring method, taking the content of magnolol and honokiol as evaluation index, and performing L9 (3) according to three-factor three-level on the main factors (A represents adjuvant usage amount, B represents temperature, and C represents baking time) influencing the processing process of the component⁴) Orthogonal test, see Table 20. orthogonal test protocol, see Table 20, visual analysis and variance of resultsSee tables 22 and 23 for analysis.

TABLE 20 factor level table

Table 21 orthogonal test protocol table

Table 22 visual analysis table

The optimal scheme is as follows: baking A2B3C1 at 80 deg.C for 3 hr with 10:1 ratio of adjuvants.

TABLE 23 ANOVA TABLE

And (4) analyzing results: the intuitive analysis table shows that the extreme difference C is more than B and more than A, so that the baking time, the baking temperature and the auxiliary material proportion influence which are the most great on the effective components of the magnolia officinalis are analyzed, and the A is obtained according to the comparison of all levels₂B₃C₁Is the optimal condition for baking magnolia officinalis with ginger juice. The optimal processing process conditions can be obtained by combining visual analysis and variance analysis, and are as follows: baking cortex Magnolia officinalis and succus Zingiberis recens at 80 deg.C for 3 hr with an adjuvant ratio of 10: 1.

4.4.2 orthogonal validation experiments

(1) Sample preparation: baking cortex Magnolia officinalis and succus Zingiberis recens at 80 deg.C for 3 hr with an adjuvant ratio of 10: 1. In triplicate.

(2) About 0.2g of ginger magnolia bark powder under the item of sample preparation is taken, three parts of test solution are prepared according to the method under the item 4.1.3, 5 mu l of the test solution is precisely absorbed and injected into a high performance liquid chromatograph, the peak area is recorded, and the measurement result is shown in the table 24.

TABLE 24 Positive verification test results

And (4) conclusion: through the positive cross validation test, the highest value of the results in the orthogonal test table can be achieved, and the optimal processing process condition selected by the orthogonal test is stable, so that the ginger-roasted magnolia officinalis process comprises the following steps: cleaning rhizoma Zingiberis recens, mashing, adding appropriate amount of water, squeezing to obtain juice, adding appropriate amount of water into rhizoma Zingiberis recens residue, squeezing once again, and mixing the juices to obtain "ginger juice". Mixing the cleaned shredded cortex Magnolia officinalis with succus Zingiberis recens, oven drying at 80 deg.C for 3 hr, taking out, and air drying. 10kg of ginger juice is used per 100kg of mangnolia officinalis.

While the invention has been described in detail in the foregoing by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that certain changes and modifications may be made therein based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. The processed magnolia officinalis product is characterized by comprising the following components in percentage by weight: the ginger juice is 2: 1-18: 1.

2. The magnolia officinalis processed product according to claim 1, wherein the processed product comprises the following components: the ginger juice is 6: 1-14: 1.

3. The magnolia officinalis processed product according to claim 2, wherein the processed product comprises the following components: the ginger juice is 8: 1-12: 1.

4. The magnolia officinalis processed product according to claim 3, wherein the processed product comprises the following components: the ratio of ginger juice to ginger juice is 10: 1.

5. The processing method of the processed magnolia bark product as set forth in any one of claims 1 to 4, wherein the method comprises:

6. The processing method of claim 5, which is characterized in that the method comprises the following steps:

7. The processing method of the magnolia officinalis processed product according to claim 6, wherein the method comprises the following steps:

8. The detection method for the processed magnolia officinalis product as claimed in any one of claims 1 to 3, wherein the detection method comprises the following steps:

1) chromatographic conditions and System suitability test

2) preparation of control solutions

3) preparation of test solution

9. The detection method for the magnolia officinalis processed product as claimed in claim 8, wherein the detection method comprises:

1) chromatographic conditions and System suitability test

2) preparation of control solutions

3) preparation of test solution

10. The detection method for the magnolia officinalis processed product as claimed in claim 9, wherein the detection method comprises:

1) chromatographic conditions and System suitability test

2) preparation of control solutions

3) preparation of test solution