CN112858273B - Intelligent hospital is with former detection kit of dog procalcitonin, detection device of adaptation - Google Patents
Intelligent hospital is with former detection kit of dog procalcitonin, detection device of adaptation Download PDFInfo
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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Abstract
The invention relates to a detection kit for an intelligent hospital, an adaptive detection device and a detection method. The kit comprises a kit body with a detection cavity, a detection plate arranged in the detection cavity and a sampling mechanism. The box body is provided with a sample adding port, and the detection plate is provided with one end communicated with the sample adding port. The detection plate is used for acquiring sample liquid from the sample adding port, processing and reacting the sample liquid and outputting optical detection information. The reagent kit overcomes the defects that the reagent performance is unstable, the accuracy and the repeatability are poor and the like caused by sample pollution in the traditional sampling mode through a special sampling mechanism.
Description
Technical Field
The invention relates to the technical field of canine procalcitonin detection equipment, in particular to a canine procalcitonin detection kit for intelligent hospitals, an adaptive detection device and a detection method.
Background
Since the 80 s of the last century, immunological detection techniques have evolved rapidly with the maturation of monoclonal antibodies, artificial skin, genetic engineering and various labeling techniques, with the use of immunoturbidimetry, rate-of-scattering nephelometry (INA), latex-enhanced turbidimetry (ITA) and chemiluminescent analytical techniques gradually replacing traditional immunoprecipitation, immunoagglutination, making detection faster, and the use of rapid detection (POCT) in recent years, making immunological procedures simpler and more convenient. The detection method of POCT changes the traditional gel layer analysis method and high performance liquid chromatography which are not easy to be automated in time consuming, and the common immunological detection method comprises the following steps: the ELISA is a closed type chemiluminescence detection system which utilizes the principles of two double antibody sandwich methods to detect indexes in serum, has more specificity and no cross reaction, but has low detection sensitivity, narrow linear range, difficult realization of full automation and the like.
In recent years, along with the development of POCT technology, the fluorescent immunochromatography technology has been developed greatly, overcomes the defects of the GICA and retains the advantages of rapidness, simplicity and convenience, and the advantages of high sensitivity and high specificity of the technology make the technology well applied in the field. However, the detection kit based on the integrated flexible and activated POCT has little influence on the use efficiency and application range, and the existing kit is greatly influenced by the environment and has low reliability.
Disclosure of Invention
In view of the above, it is necessary to provide a detection kit for intelligent hospitals, an adaptive detection device and a detection method. The kit is convenient to operate, can reduce environmental interference and is high in reliability.
The invention provides a detection kit for intelligent hospitals, which comprises the following components:
the box body forms a detection cavity, and a sample adding port is formed in the box body;
the detection plate is arranged in the detection cavity and is provided with one end communicated with the sample adding port, and the detection plate is used for acquiring sample liquid from the sample adding port, processing and reacting the sample liquid and outputting optical detection information;
the sealing pad is arranged at the sample adding port;
and the sampling mechanism comprises a sampling shell and a valve body, the sampling shell is provided with a sampling section, a connecting section, a discharging section and an extruding part, the sampling section is used for sucking external sample liquid, the connecting section is used for communicating the sampling port, the discharging section is used for discharging waste liquid, the extruding part is arranged between the sampling section and the connecting section, the sampling section to the connecting section forms a sampling passage, the sampling section to the discharging section forms a discharging passage, the sampling passage and the discharging passage are respectively provided with a valve body, the valve body on the sampling passage can prevent the sample liquid from flowing from the connecting section to the sampling section, and the valve body on the discharging passage can prevent the sample liquid from flowing from the discharging section to the sampling section.
Further, the sampling mechanism further comprises a power source in transmission connection with the valve body, and the power source drives the valve body to move in the sample injection passage or the discharge passage.
Optionally, the power supply is extrusion portion, extrusion portion includes be suitable for the extrusion hand protruding and connect in hand protruding with the elastic component between the valve body, hand protruding can be pressed and warp, and pass through the elastic component drives the valve body action.
Further, the elastic member includes an extension section disposed along the length direction of the sample introduction path or the discharge path, and a compression section extending into the sampling case corresponding to the hand-shaped protrusion, where the compression section is deformable together with the hand-shaped protrusion to drive the extension section to extend or retract along the length direction of the sample introduction path or the discharge path.
Optionally, the power source is the movable ring of movable sleeve on the sampling shell, the valve body set up in being close to the movable ring in the sampling shell, the movable ring with the valve body passes through magnetic force transmission and is connected.
Further, the sampling shell is further provided with a filtering part arranged on the sampling passage, the sampling shell further comprises an air head communicated to the sampling passage or the discharging passage, and the air head can perform sterilization filtration on the air entering the sampling passage or the discharging passage or balance the air pressure in the sampling passage or the discharging passage.
The invention also provides a detection device matched with the kit, which comprises:
the box body is provided with a box body,
the detection main body is arranged in the box body, the top end of the detection main body is used for being detachably connected with the kit, the bottom end of the detection main body is provided with a temperature control part and a detection part, the detection part is used for acquiring optical biological information on a detection plate in the kit, and the temperature control part is used for controlling the temperature in the kit connected to the top end of the detection main body;
and the control console is fixedly connected with the box body, is respectively and electrically connected with the detection part and the temperature control part, and is used for acquiring information of the detection part and the temperature control part and sending control instructions to the detection part and the temperature control part.
Further, the detection main body is also provided with a vibration part for uniformly vibrating and mixing the sample.
The invention also provides a detection method by adopting the kit or the detection device, which comprises the following steps:
s1, preparing the detection plate, mounting the detection plate in a detection cavity, and sealing a sampling port of the shell by adopting a sealing gasket;
s2, when detection is needed, taking the sampling mechanism, and connecting the connecting section with the sample adding port;
s3, obtaining sample liquid through the extrusion part, driving the valve body on the sample injection passage to act, and enabling the sample liquid to enter the sample injection passage and flow to the detection plate in a communicating way;
s4, detecting the reaction by the detection plate through the reactant attached to the detection plate, and acquiring a detection result through a self-arranged command line or by means of external detection equipment.
Further, the detection method further comprises the steps of performing temperature control and detection on the detection reaction.
The beneficial effects are that:
1. the kit provided by the invention can be used for CPCT rapid quantitative analysis in serum, plasma, peripheral blood, venous blood and the like, and has the advantages of rapidness, simplicity, convenience, strong anti-interference capability and the like; the special sampling mechanism overcomes the defects of unstable reagent performance, poor accuracy, poor repeatability and the like caused by sample pollution in the traditional sampling mode, greatly improves the stability of products, has more accurate and reliable detection results, has better repeatability and linearity, provides support for diagnosis, differential diagnosis, treatment effect monitoring and post-healing evaluation of bacterial infection, and has good application prospect.
2. When the detection device is carried, the kit can be inserted into the top end of the detection main body, and the detection main body is recovered after the sampling and sample reaction work is completed through the kit; in addition, the detection part can be used for obtaining the biochemical reaction result information generated in the kit, such as the color development, fluorescence development, chemiluminescence and absorbance measurement of a control line. The kit can be used for carrying out rapid detection independently, and for detecting some complex biological samples, the kit can be matched with a detection device for carrying out rapid detection, and the application range of the kit is greatly widened through the matched use of the kit and the detection device.
3. When detecting complicated biological sample, often need carry out temperature control to it, current detection kit itself does not set up any article parking space except the test tube, thereby it is reserve to lead to the test personnel unable to deposit some auxiliary material when using, its detection kit itself is inside not to set up refrigerated construction simultaneously, thereby it can't effectively store the work to its inside medicine to lead to the kit itself, and do not set up any supplementary test structure that dispenses in the general detection kit, thereby the test personnel need consume a large amount of time in the work of dispensing before detecting, and can't ensure the quality of dispensing, reduce the practical degree of kit itself. The kit and the detection device provided by the invention can be used for refrigerating samples, carrying out sample treatment and reaction in real time, collecting biological information on detection results, facilitating the use and observation of medicines by later-stage experimenters, effectively avoiding the consumption of a large amount of dispensing time by experimenters before experimental detection work, ensuring the dispensing quality, and reducing the practical degree of the kit.
Drawings
FIG. 1 is a schematic diagram of a detection kit for a smart hospital according to an embodiment of the present invention
Fig. 2 is a schematic diagram showing structural connection of the squeeze part and the valve in the kit according to the embodiment of the present invention.
Fig. 3 is another state diagram of fig. 2.
Fig. 4 is a schematic perspective view of a valve in the kit according to an embodiment of the present invention.
Fig. 5 is a schematic diagram showing alternative structural connection of the moving ring and the valve in the kit according to the embodiment of the present invention.
Fig. 6 is a schematic diagram showing an alternative structural connection of a moving ring and a valve in the kit according to an embodiment of the present invention.
Fig. 7 is a schematic diagram showing an alternative structural connection of a moving ring and a valve in the kit according to an embodiment of the present invention.
Fig. 8 is a schematic diagram showing alternative structural connection of the moving ring and the valve in the kit according to the embodiment of the present invention.
Fig. 9 is a schematic structural diagram of a detection device adapted to the kit according to an embodiment of the present invention.
Fig. 10 is a flowchart of a detection method using the kit or the detection device according to an embodiment of the present invention.
Fig. 11 is an alternative flowchart of step S3 in fig. 10.
Fig. 12 is an alternative flowchart of step S3 in fig. 10.
1 box body, 10 detection cavity, 100 sample suction cavity, 101 reaction cavity, 11 sample inlet, 12 discharge outlet, 2 detection plate, 3 sealing pad,
4 sampling mechanism, 40 sampling shell, 400 sample sucking section, 4000 annular bulge, 401 connecting section, 402 discharging section, 403 extruding section, 4030 hand bulge, 4031 elastic piece, 4032 extending section, 4033 compression section,
41 valve body, 410 valve portion, 4100 mounting portion, 411 sliding ring, 412 valve core,
A 42 ring, 420 ring-shaped locking grooves, an A sample introduction passage, a B discharge passage, a 43 gas head, a 44 first spring, a 45 second spring,
5 boxes, 6 detection main bodies, 60 temperature control parts, 600 placing grooves, 61 detection parts and 7 control tables.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the following examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The embodiment of the invention provides a detection kit for an intelligent hospital, which comprises a box body 1, a detection plate 2, a sealing pad 3 and a sampling mechanism 4. The kit provided by the invention can be used for CPCT rapid quantitative analysis in serum, plasma, peripheral blood, venous blood and the like, and has the advantages of rapidness, simplicity, convenience, strong anti-interference capability and the like; the special sampling mechanism overcomes the defects of unstable reagent performance, poor accuracy, poor repeatability and the like caused by sample pollution in the traditional sampling mode, greatly improves the stability of products, has more accurate and reliable detection results, has better repeatability and linearity, provides support for diagnosis, differential diagnosis, treatment effect monitoring and post-healing evaluation of bacterial infection, and has good application prospect.
Referring to fig. 1, a detection cavity 10 is formed in a box body 1, a sample inlet 11 is formed in the box body 1, and the sample inlet 11 is communicated with the inside and the outside of the detection cavity 10.
The detection plate 2 is disposed in the detection chamber 10. The detection plate 2 has one end communicating with the sample addition port 11. The detection plate 2 is used for acquiring a sample liquid from the sample inlet 11, processing and reacting the sample liquid and outputting optical detection information. The test board 2 may be a common test board with test paper function.
Taking the detection of the canine procalcitonin as an example, the detection plate comprises a PVC (polyvinyl chloride) bottom lining plate, a nitrocellulose membrane, a water absorption pad and a sample treatment pad, wherein the nitrocellulose membrane, the water absorption pad and the sample treatment pad are all adhered to the PVC bottom lining plate, the nitrocellulose membrane is positioned in the middle, the sample treatment pad and the water absorption pad are respectively lapped at two ends of the nitrocellulose membrane, the middle of the nitrocellulose membrane is parallel provided with a detection line coated with a canine procalcitonin polyclonal antibody and a quality control line coated with a sheep anti-mouse IgG secondary antibody, and an observation window right opposite to the middle of the nitrocellulose membrane is arranged on the box body. The sample treatment pad comprises a whole blood filter membrane treated by a treatment liquid and a glass fiber membrane treated by a red cell monoclonal antibody, wherein the glass fiber membrane is arranged on the outer layer, the whole blood filter membrane is arranged on the inner layer, and the glass fiber membrane and the whole blood filter membrane are subjected to sealing and drying treatment under the condition that the relative humidity of the glass fiber membrane and the whole blood filter membrane is not higher than 30 percent at room temperature. The treatment solution is a Tri-HCl buffer solution containing 0.2-1% Tween-20, 0.1-2% BSA and 0.5-1% blocker, and pH 7.0-8.0, and the erythrocyte monoclonal antibody treatment solution is a Tri-HCl buffer solution containing 0.2-1% Tween-20, 0.1-2% BSA and 0.5-3 mg/mL anti-Hb monoclonal antibody, and pH 7.0-8.0. The detection line L-T and the quality control line L-C are formed by parallelly scribing on a nitrocellulose membrane by a membrane spraying instrument at a spraying speed of 1-2uL/cm and a distance of 0.7cm, wherein two antibody solutions used by the membrane spraying instrument are respectively an antibody solution prepared by preparing an anti-canine procalcitonin polyclonal antibody by using a Tri-Cl buffer solution with the concentration of 0.02M and the pH of 7.0 to 1-2 mg/mL, an antibody solution prepared by preparing a goat anti-mouse IgG secondary antibody by using a Tri-HCl buffer solution with the concentration of 0.02M and the pH of 7.0, and the nitrocellulose membrane after the membrane is sprayed is dried and stored in a sealing manner under the condition that the relative humidity of the nitrocellulose membrane at room temperature is not higher than 30%.
The closing pad 3 is arranged at the sample adding port 11. The detection chamber 10 for the closing pad 3 is sealed, reducing the contact of the detection plate 2 with the outside. Further, the sealing pad 3 can be pierced and kept sealed in the detection chamber 10.
As shown in fig. 1, the sampling mechanism 4 includes a sampling housing 40 and a valve 41. The sampling housing 40 includes a sample suction section 400, a connection section 401, a discharge section 402, and a pressing portion 403. The sample-sucking section 400 may suck the external sample liquid. The connecting section 401 can be connected to the sample inlet 11, and thus, after connection, the sealing is performed by the sealing pad 3. The discharge section 402 is used to discharge waste liquid. The pressing portion 403 is provided between the sample absorbing stage 400 and the connecting stage 401. The sample sucking section 400 to the connecting section 401 form a sample injection passage A, and the sample sucking section 400 to the discharging section 402 form a discharging passage B. The sample introduction path A and the discharge path B are both provided with a valve 41, the valve 41 positioned on the sample introduction path A can prevent sample liquid from flowing from the connecting section 401 to the sample sucking section 400, and the valve 41 positioned on the discharge path B can prevent sample liquid from flowing from the discharge section 402 to the sample sucking section 400. In this way, the connecting section 401 can be communicated with the sample adding port 11 through the sampling mechanism 4 under the requirement that the sealing sampling is needed to reduce the influence of the environment, so that the sample liquid can be subjected to closed sampling through the sampling mechanism, and the opportunity of personnel and environment contact is reduced.
Further, to facilitate the absorption of the sample liquid by the detection plate, the detection chamber 10 includes a sample absorbing chamber 100 and a reaction chamber 101. One end of the detection plate 2 extends into the sample absorbing chamber 100 for absorbing the sample liquid. The other end of the detection plate 2 extends into the reaction chamber 101, and is used for transferring the absorbed sample liquid into the reaction chamber 101 through the capillary attraction generated by the detection plate 2 and reacting with the coupling reaction substance on the detection plate 2 at one end in the reaction chamber 10, so as to achieve the purpose of detection.
Further, in order to facilitate the sample in the sample absorbing chamber 100 to be sufficiently absorbed by the detection plate 2, the detection plate 2 includes a substrate 20 and an absorbing layer 21 attached to the substrate 20.
Optionally, the substrate 20 is provided with a plurality of capillary grooves, and the absorbing layer 21 has one end attached to the inside of the capillary grooves. Optionally, the substrate 20 is disposed in the sample chamber 100 at one end thereof in a mesh structure, and the absorbent layer 21 has a portion attached between the pores of the mesh structure.
Correspondingly, as shown in fig. 1, the sample suction cavity 100 is provided with a sample inlet 11, and the sample suction section 400 can be communicated with the sample inlet 11; the sample absorbing cavity 100 is further provided with a discharge port 12, and the discharge section 402 can be communicated with the discharge port 12, so that the sample liquid is absorbed by the sample absorbing section 400, enters the sample absorbing cavity 100 through the sample adding port 11, is fully absorbed by the absorbing layer 21 in the sample absorbing cavity 100, flows out of the sample absorbing cavity 100 through the discharge port 12, and is discharged through the discharge section 402.
Furthermore, the reaction chamber 101 may further contain a fluorescent reagent, by disposing the extrusion portion 403 on the reaction chamber 101 housing, the fluorescent reagent is wrapped in a reagent groove disposed in the reaction chamber 101 by a sealing film, and the inner wall of the reaction chamber 101 housing corresponding to the fluorescent reagent is protruded to form a hook, when the extrusion portion 403 is pressed, the hook may pierce the sealing film to release the fluorescent reagent to the detection plate 2 in the reaction chamber 101, so as to perform fluorescent reaction or fluorescent display, thereby facilitating detection and judgment of the reaction.
In particular, the sampling mechanism 4 further comprises a power source in driving connection with the valve 41. The power source driving valve 41 moves in the injection passage a or the discharge passage B, thereby achieving the function of blocking, choking or guiding the injection passage a or the discharge passage B.
Alternatively, the power source is the pressing portion 403. Specifically, the pressing portion 403 includes a hand-shaped protrusion 4030 adapted to press and an elastic member 4031 connected between the hand-shaped protrusion 4030 and the valve 41. The hand-shaped protrusion 4030 is deformed by pressure and drives the valve 41 to act through the elastic member 4031.
Specifically, as shown in fig. 2 and 3, the elastic member 4031 includes an extension section 4032 provided along the length direction of the sample introduction path a or the discharge path B and a compression section 4033 extended into the sample housing 40 corresponding to the hand-shaped protrusion 4030. The compression section 4033 can deform along with the hand-held protrusion 4030 to drive the extension section 4032 to extend or retract along the length of the sample introduction path a or the discharge path B.
Alternatively, the power source is a movable ring 42 movably sleeved on the sampling housing 40. The valve 41 is disposed within the sampling housing 40 adjacent to the moving ring 42, and the moving ring 42 is magnetically coupled to the valve 41. Specifically, the moving ring 42 may act on the valve 41 by magnetic attraction and repulsion forces to urge the valve 41 to move to block, choke or guide the injection passage a or the discharge passage B.
Further, the movable ring 42 has one end elastically connected to the outer wall of the sampling case 40, so that the movable ring 42 can be elastically connected to the outer wall of the sampling case 40 when it is not driven by an external force. Further, to lock the moving ring 42 against the outer wall of the sampling shell 40, the moving ring 42 is further provided with an annular locking groove 420, and the sampling shell 40 further has an annular protrusion 4000 matching with the annular locking groove 420. When the moving ring 42 moves relative to the outer wall of the sampling shell 40 until the annular protrusion 4000 is clamped in the annular locking groove 420, locking of the moving ring 42 relative to the outer wall of the sampling shell 40 is achieved. Specifically, the annular protrusion 4000 or the annular locking groove 420 is made of an elastic material, and can be deformed by pressing so as to enable the annular protrusion and the annular locking groove to slide relatively.
Specifically, the sampling housing 40 has a valve portion 410 adapted to actuate the valve 41, and the valve portion 410 has an inner wall adapted to the outer surface of the valve 41. As shown in fig. 4, the valve 41 is cylindrical at one end and conical at the other end, the inner wall of the valve portion 410 is adapted to the valve 41, and when the conical one end of the valve 41 driven by the moving ring 42 is pressed against the inner wall of the valve body 400, the flow of liquid in the valve portion 410 is stopped; when the conical end of the valve 41 is driven by the movable ring 42 to leave the inner wall of the valve portion 410, the liquid in the valve portion 410 resumes circulation. Specifically, as shown in fig. 4, the valve 41 includes a sliding ring 411 and a valve core 412 extending from one side of the center of the sliding ring 411, where the sliding ring 411 is adapted to the inner wall of the sampling shell 40, and can enable fluid to flow through, and the sliding ring 411 is further connected to an elastic member 4031, and can be driven by the elastic member 4031 to slide along the direction of the sampling path a, so that the valve core 412 can be pressed against the valve portion 410 to realize opening and closing of the valve. More specifically, one end of the valve element 412 is cone-shaped, and the valve element 412 is made of an elastic rubber material so as to firmly close the valve portion 410.
More specifically, in the embodiment in which the power source is the pressing portion 403, the elastic member 4031 is connected to a cylindrical end of the valve 41, and a conical end of the valve 41 is used for opening and closing the valve portion 410.
More specifically, in the embodiment in which the power source is the movable ring 42, as shown in fig. 5 to 8, the valve portion 410 is formed with the mounting portion 4100 outside, and the movable ring 42 is movably connected to the mounting portion 4100. For example, the movable ring 42 is connected to the mounting portion 4100 by a first spring 44, the mounting portion 4100 is an annular groove structure on the outer periphery of the sampling shell 40, and a grating opening is formed along the length direction of the sampling shell 40, and the movable ring 42 has a portion extending from the grating opening to the outer periphery of the sampling shell 40, so that the movable ring 42 can be pushed by a user to slide. And the sliding ring 411 is elastically coupled to the inner wall of the sampling path a by the second spring 45. And the sliding ring 411 is magnetically repulsive to the moving ring 42.
Further, the sampling case 40 is further formed with a filter part 402 provided on the sample introduction path a. The sampling mechanism 4 further includes a gas head 43 that communicates into the sample introduction path a or the discharge path B. The air head 43 may filter the sterilization into the sample introduction path a or the discharge path B, or balance the air pressure in the sample introduction path a or the discharge path B.
The invention also provides a detection device matched with the kit provided by the embodiment, as shown in fig. 9, comprising a box body 5, a detection main body 6 and a control console 7. The box 5 forms a sealed space, and the detection main body 6 and the control console 7 are arranged in the box, so that the box is convenient to transport and carry by a user.
The detection body 6 is provided in the case 5. The tip of the detection body 6 is used to detachably attach the kit provided in the above embodiment. The bottom end of the detection body 6 has a temperature control portion 60 and a detection portion 61. The detection section 61 is used to acquire optical biological information on a detection plate in the kit. The temperature control unit 61 is used to control the temperature in the kit connected to the tip of the detection body 6.
The console 7 is fixedly installed in the sealed space of the case 1. The console 7 is electrically connected with the detecting part and the temperature controlling part, and is used for acquiring information of the detecting part and the temperature controlling part and sending control instructions to the detecting part and the temperature controlling part.
Thus, when the detection device is carried, the kit can be inserted into the top end of the detection main body 6, and the detection main body is recovered after the sampling and sample reaction work is completed through the kit; in addition, the detection part can be used for obtaining the biochemical reaction result information generated in the kit, such as the color development, fluorescence development, chemiluminescence and absorbance measurement of a control line. For some detection reactions, the temperature needs to be controlled, for example, for the detection of some nucleic acid samples, the existing detection kit is not provided with any article storage space except a test tube, so that a tester cannot store some auxiliary equipment for later use when using, meanwhile, the inside of the detection kit is not provided with a refrigeration structure, so that the kit cannot effectively store medicines in the kit, and no auxiliary dispensing test structure is arranged in the general detection kit, so that the tester needs to consume a large amount of time on dispensing work before detection, the dispensing quality cannot be ensured, and the practical degree of the kit is reduced.
Specifically, the temperature control unit 61 in the present application can spray cool air and warm air to the kit that is locked to the top end of the detection body 6. Specifically, the placement groove 600 is formed at the top end of the detection main body 6, the kit can be placed in the placement groove 600, and a temperature control area 601 is formed in the placement groove 600. The temperature control part 61 includes a hot air piece and a cold air piece provided in the inner wall of the case 1. The hot air part can blow hot air into the placement groove 600 by electric heating. The cold air piece is released by releasing pressure by a cold source with a storage, so as to blow cold air into the placing groove 600, wherein the cold source can be high-pressure liquid nitrogen, liquid helium or liquid carbon dioxide, or solid ice (which is in direct contact with the placing groove 600 so as to be convenient for cooling).
Further, the detection main body 6 further has a vibration part 62 for uniformly vibrating and mixing the sample. Specifically, the vibration part 62 includes a vibration motor disposed in the case 1 and a vibration connector in driving connection with a power output end of the vibration motor. The vibration connecting piece is fixedly connected with the placing groove 600, and the placing groove 600 can be used for placing not only the sample tube but also the reagent kit.
Further, in some special detection, at first the experimenter can put into standing groove 600 with test tube or sample tube that is equipped with the medicine, closed box 1, afterwards start vibrating motor through pressing the vibration motor control button on control cabinet 4, drive vibrations board connecting piece through vibrating motor and vibrate, and vibrate the mixed work of medicine in to the test tube or the sample tube in the standing groove 600 through vibrating connecting piece, can effectively accelerate the interior molecular activity rate of medicine, thereby improve its mixed quality, the later stage experimenter of being convenient for uses and observes the medicine, effectively avoid the experimenter to consume a large amount of dispensing time before experimental detection work, and can't ensure the quality of dispensing, reduce the practical degree of kit itself.
Further, the box 1 includes the case lid, the top both sides fixedly connected with closure spare of case lid, through the closure spare, can seal and block up standing groove 600 respectively after the case lid is closed, play the effect in separate space, avoid hot-blast spare or cold wind spare to cause the damage to standing groove or detect the relevant part of main part, make it appear fog when taking out the use and take place the oxidation phenomenon, also can improve detection kit device main part 1 self energy consumption problem simultaneously greatly, reduce its consumption, improve its life.
The invention also provides a detection method using the kit or the detection device, as shown in fig. 10, comprising the following steps:
s1, preparing the detection plate, mounting the detection plate in a detection cavity, and sealing a sampling port of the shell by adopting a sealing gasket;
s2, when detection is needed, taking the sampling mechanism, and connecting the connecting section with the sample adding port;
s3, obtaining sample liquid through the extrusion part, driving the valve body on the sample injection passage to act, and enabling the sample liquid to enter the sample injection passage and flow to the detection plate in a communicating way;
s4, detecting the reaction by the detection plate through the reactant attached to the detection plate, and acquiring a detection result through a self-arranged command line or by means of external detection equipment.
Taking the detection plate provided above as an example, the detection plate preparation step in the S1 step includes:
the detection plate comprises a PVC bottom lining plate, a nitrocellulose membrane, a water absorption pad and a sample treatment pad, wherein the nitrocellulose membrane, the water absorption pad and the sample treatment pad are all adhered to the PVC bottom lining plate, the nitrocellulose membrane is positioned in the middle, the sample treatment pad and the water absorption pad are respectively lapped at two ends of the nitrocellulose membrane, and two control lines are arranged in parallel in the middle of the nitrocellulose membrane. The sample treatment pad comprises a whole blood filter membrane treated by treatment liquid and a glass fiber membrane treated by red cell monoclonal antibody treatment liquid, wherein the glass fiber membrane is arranged on the outer layer, and the whole blood filter membrane is arranged on the inner layer; and sealing and drying the glass fiber membrane and the whole blood filter membrane under the condition that the relative humidity at room temperature is not higher than 30 percent.
Wherein the treatment solution is a Tri-HCl buffer solution containing 0.2-1% Tween-20, 0.1-2% BSA and 0.5-1% blocker, and the erythrocyte monoclonal antibody treatment solution is a Tri-HCl buffer solution containing 0.2-1% Tween-20, 0.1-2% BSA and 0.5-3 mg/mL anti-Hb monoclonal antibody, and 0.02M, pH 7.0-8.0. The detection line L-T and the quality control line L-C are formed by parallelly scribing on a nitrocellulose membrane by a membrane spraying instrument at a spraying speed of 1-2uL/cm and a distance of 0.7cm, wherein two antibody solutions used by the membrane spraying instrument are respectively an antibody solution prepared by preparing an anti-canine procalcitonin polyclonal antibody by using a Tri-Cl buffer solution with the concentration of 0.02M and the pH of 7.0 to 1-2 mg/mL, an antibody solution prepared by preparing a goat anti-mouse IgG secondary antibody by using a Tri-HCl buffer solution with the concentration of 0.02M and the pH of 7.0, and the nitrocellulose membrane after the membrane is sprayed is dried and stored in a sealing manner under the condition that the relative humidity of the nitrocellulose membrane at room temperature is not higher than 30%.
In an alternative embodiment of step S3, as shown in fig. 11, the method includes:
s31, the power source is an extrusion part, the extrusion part comprises a hand-shaped bulge suitable for extrusion and an elastic piece connected between the hand-shaped bulge and the valve body, the hand-shaped bulge is held to deform the hand-shaped bulge, and the valve body is driven to act through elasticity so as to open the sample introduction passage;
s32, the detection cavity comprises a sample suction cavity and a reaction cavity, one end of the detection plate extends into the sample suction cavity and is used for absorbing sample liquid, the other end of the detection plate extends into the reaction cavity, the extrusion part absorbs the sample, and the absorbed sample liquid can migrate to the reaction cavity through capillary absorption generated by the detection plate and react with coupling reaction substances on the detection plate positioned at one end of the reaction cavity so as to achieve the detection purpose.
In another alternative embodiment of step S3, as shown in fig. 12, the method includes:
s31, the power source is a movable ring movably sleeved on the sampling shell, the valve body is arranged in the sampling shell close to the movable ring, the movable ring is connected with the valve body through magnetic force transmission, the movable ring is pushed to move relative to the outer wall of the sampling shell 40, and the movable ring can act with the valve body through magnetic attraction and repulsion force so as to promote the valve body to move, so that a sampling passage is opened;
s32, the detection cavity comprises a sample suction cavity and a reaction cavity, one end of the detection plate extends into the sample suction cavity and is used for absorbing sample liquid, the other end of the detection plate extends into the reaction cavity, the extrusion part absorbs the sample, and the absorbed sample liquid can migrate to the reaction cavity through capillary absorption generated by the detection plate and react with coupling reaction substances on the detection plate positioned at one end of the reaction cavity so as to achieve the detection purpose.
Further, the detection method further comprises the steps of performing temperature detection on the detection reaction. Specifically, the temperature control can be realized by spraying cool air and warm air to the kit clamped at the top end of the detection main body through the control Wen Buxiang. The hot air part can blow hot air into the placement groove 600 by electric heating. The cold air piece is released by releasing pressure by a cold source with a storage, so as to blow cold air into the placing groove 600, wherein the cold source can be high-pressure liquid nitrogen, liquid helium or liquid carbon dioxide, or solid ice (which is in direct contact with the placing groove 600 so as to be convenient for cooling). Therefore, the control console can be used for controlling the hot air piece and the cold air piece to control the hot air quantity and the cold air quantity blown out by the hot air piece and the cold air piece, and further control the temperature of the kit. The detecting step may be implemented by using a detecting unit provided by the detecting body, for example, some optical lenses or detecting modules are provided, so as to accurately obtain the optical biological information on the detecting board, so as to determine the detecting result.
The present invention is not limited to the above-mentioned embodiments, and any changes or substitutions that can be easily understood by those skilled in the art within the technical scope of the present invention are intended to be included in the scope of the present invention.
Claims (3)
1. A kit for detecting procalcitonin in a canine for a smart hospital, comprising:
the box body is provided with a sample adding port, and the detection cavity comprises a sample sucking cavity and a reaction cavity which are partially separated;
the detection plate is arranged in the detection cavity, one end of the detection plate extends into the sample suction cavity and is communicated with the sample adding port and is used for absorbing sample liquid, the other end of the detection plate extends into the reaction cavity and is used for transferring the absorbed sample liquid into the reaction cavity through capillary suction generated by the detection plate and reacting with a coupling reaction substance on the detection plate at one end of the reaction cavity so as to process and react the sample liquid and output optical detection information; the detection plate comprises a PVC bottom lining plate, a nitrocellulose membrane, a water absorption pad and a sample treatment pad, wherein the nitrocellulose membrane, the water absorption pad and the sample treatment pad are all adhered to the PVC bottom lining plate, the nitrocellulose membrane is positioned in the middle, the sample treatment pad and the water absorption pad are respectively lapped at two ends of the nitrocellulose membrane, the middle of the nitrocellulose membrane is provided with a detection line coated with a canine procalcitonin antigen and a quality control line coated with a goat anti-mouse IgG secondary antibody in parallel, and the box body is provided with an observation window right opposite to the middle of the nitrocellulose membrane; the sample treatment pad comprises a whole blood filter membrane treated by a treatment liquid and a glass fiber membrane treated by erythrocyte monoclonal antibody, wherein the glass fiber membrane is arranged on the outer layer, the whole blood filter membrane is arranged on the inner layer, and the glass fiber membrane and the whole blood filter membrane are subjected to sealing and drying treatment under the condition that the relative humidity of the whole blood filter membrane is not higher than 30% at room temperature; the treatment solution is a Tri-HCl buffer solution containing 0.2-1% Tween-20, 0.1-2% BSA and 0.5-1% blocker, wherein the red blood cell monoclonal antibody treatment solution is a Tri-HCl buffer solution containing 0.02M, pH 7.0-8.0 of anti-Hb monoclonal antibody, 0.2-1% Tween-20, 0.1-2% BSA and 0.5-3 mg/mL; the detection line and the quality control line are formed by parallel scribing on the nitrocellulose membrane at the spraying speed of 1-2uL/cm and the distance of 0.7cm by a membrane spraying instrument;
the sealing pad is arranged at the sample adding port; and
A sampling mechanism, the sampling mechanism comprising:
the sampling shell is provided with a sample sucking section, a connecting section, a discharging section and an extruding section, wherein the sample sucking section is used for sucking external sample liquid, the connecting section is used for communicating the sample adding port, the discharging section is used for discharging waste liquid, the extruding section is arranged between the sample sucking section and the connecting section, and a sample injecting passage is formed from the sample sucking section to the connecting section;
the valve body is arranged on the sample introduction passage and the discharge section, the valve body positioned on the sample introduction passage can prevent sample liquid from flowing from the connecting section to the sample suction section, and the valve body positioned on the discharge section can prevent sample liquid from flowing from the discharge section to the sample suction section; the valve body comprises a sliding ring and a valve core extending from one side of the center of the sliding ring, the sliding ring is matched with the inner wall of the sampling shell and can enable fluid to flow through, and one end of the valve core is cone-shaped so as to firmly close the valve body;
the power source is in transmission connection with the valve body and drives the valve body to move in the sample injection passage;
the power source is an extrusion part, the extrusion part comprises a hand-shaped protrusion suitable for extrusion and an elastic piece connected between the hand-shaped protrusion and the valve body, the hand-shaped protrusion can be deformed under pressure, and the valve body is driven to act through the elastic piece;
the elastic piece comprises an extending section and a pressed section, the extending section is arranged along the length direction of the sample injection passage, the pressed section extends into the sampling shell corresponding to the hand-shaped protrusion, and the pressed section can deform along with the hand-shaped protrusion to drive the extending section to extend or shrink along the length direction of the sample injection passage.
2. The kit of claim 1, wherein the sampling housing is further formed with a filter portion disposed on the sampling passage, and the sampling mechanism further comprises an air head communicated into the sampling passage or the exhaust section, the air head being capable of sterilizing and filtering the air entering the sampling passage or the exhaust section, or balancing the air pressure in the sampling passage or the exhaust section.
3. A test device for testing using the kit of claim 1 or 2, the test device comprising:
a case;
the kit comprises a detection main body arranged in a box body, wherein the top end of the detection main body is used for detachably connecting the kit of claim 1 or 2, the bottom end of the detection main body is provided with a temperature control part and a detection part, the detection part is used for acquiring optical biological information on a detection plate in the kit, the temperature control part is used for controlling the temperature in the kit connected to the top end of the detection main body, and the detection main body is also provided with a vibration part for uniformly vibrating and mixing a sample; and
The control console is fixedly connected with the box body, is respectively and electrically connected with the detection part and the temperature control part, and is used for acquiring information of the detection part and the temperature control part and sending control instructions to the detection part and the temperature control part.
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