CN112852601A - Intestinal bacterial colony screening filtration equipment - Google Patents
Intestinal bacterial colony screening filtration equipment Download PDFInfo
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- CN112852601A CN112852601A CN202110018713.0A CN202110018713A CN112852601A CN 112852601 A CN112852601 A CN 112852601A CN 202110018713 A CN202110018713 A CN 202110018713A CN 112852601 A CN112852601 A CN 112852601A
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- tank
- fixed
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- bacterial colony
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- 230000001580 bacterial effect Effects 0.000 title claims abstract description 31
- 238000001914 filtration Methods 0.000 title claims abstract description 28
- 238000012216 screening Methods 0.000 title claims abstract description 13
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 44
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 33
- 238000010790 dilution Methods 0.000 claims abstract description 21
- 239000012895 dilution Substances 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 16
- 238000007865 diluting Methods 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000002699 waste material Substances 0.000 claims abstract description 5
- 241001052560 Thallis Species 0.000 abstract description 4
- 230000002550 fecal effect Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 2
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an intestinal colony screening and filtering device, which comprises: fixing the frame; a bacterial colony diluting and stirring tank for placing fresh excrement; the fourth-stage filter tank is communicated with the bacterial colony dilution stirring tank; the waste liquid storage tank is communicated with the four-stage filter tank; the stirring lifting sliding table is fixed on the fixed rack and used for inserting or keeping away the carried stirring paddle from the interior of the bacterial colony dilution stirring tank; the water adding pipeline is fixed on the fixed rack, is respectively communicated with the bacterial colony diluting and stirring tank and the four-stage filtering tank and is used for providing water; the nitrogen protection pipeline is fixed on the fixed rack, is respectively communicated with the bacterial colony dilution stirring tank and the four-stage filtering tank and is used for providing nitrogen; and the filter paper replacing sliding table is fixed on the fixed rack and used for controlling the replacement of the filter paper of each stage of filter unit in the four-stage filter tank. The device can be used for rapidly screening and filtering fresh excrement of healthy qualified donors and collecting effective thalli.
Description
Technical Field
The invention relates to an intestinal colony screening and filtering device.
Background
The fecal microbe transplanting technology is to filter and screen the fresh excrement of healthy donor and to treat intestinal tract diseases, such as intestinal tract dysfunction, metabolism disorder, etc. with beneficial thallus. Since most of the fecal bacteria are anaerobic bacteria and survive in the feces of qualified donors, it is important to rapidly screen out as many effective bacteria as possible. At present, no professional equipment is used for quickly and efficiently screening fecal bacteria.
Disclosure of Invention
The invention aims to provide an intestinal colony screening and filtering device, which is used for rapidly screening and filtering fresh excrement of a healthy qualified donor and collecting effective thalli.
The technical scheme for realizing the purpose is as follows:
an intestinal colony screening filter apparatus comprising:
fixing the frame;
the bacterial colony dilution stirring tank is fixed on the fixed frame and used for placing fresh excrement;
the fourth-stage filter tank is arranged at the bottom of the bacterial colony diluting and stirring tank and is communicated with the bacterial colony diluting and stirring tank through a valve at the bottom of the bacterial colony diluting and stirring tank;
the waste liquid storage tank is arranged at the bottom of the four-stage filter tank and is communicated with the four-stage filter tank;
the stirring lifting sliding table is fixed on the fixed rack and used for inserting or keeping away the carried stirring paddle from the interior of the bacterial colony dilution stirring tank;
the water adding pipeline is fixed on the fixed rack, is respectively communicated with the bacterial colony diluting and stirring tank and the four-stage filtering tank and is used for providing water;
the nitrogen protection pipeline is fixed on the fixed rack, is respectively communicated with the bacterial colony dilution stirring tank and the four-stage filtering tank and is used for providing nitrogen; and
and the filter paper replacing sliding table is fixed on the fixed rack and used for controlling the replacement of the filter paper of each stage of filter unit in the four-stage filter tank.
Preferably, the four-stage filter tank comprises a first-stage filter unit, a second-stage filter unit, a third-stage filter unit and a four-stage filter unit which are connected in sequence.
Preferably, the method further comprises the following steps: the motor is arranged on the stirring lifting sliding table and used for driving the stirring paddle to rotate;
and the negative pressure air exhaust system is fixed on the fixed rack and is used for vacuumizing the four-stage filter tank.
Preferably, the method further comprises the following steps: and the control system is arranged on the fixed rack and used for controlling the stirring lifting sliding table, the water adding pipeline, the nitrogen protection pipeline, the filter paper replacing sliding table, the motor and the negative pressure air pumping system.
The invention has the beneficial effects that: the method quickly screens and filters the fresh excrement of the healthy qualified donor, collects effective thalli, has the characteristics of simple operation, quick removal of harmful food residues, high thallus collection and survival rate and the like, and effectively fills the gap of the market. Has high practicability.
Drawings
Fig. 1 is a perspective view of an intestinal colony screening filter apparatus of the present invention.
Detailed Description
The invention will be further explained with reference to the drawings.
Referring to fig. 1, the intestinal colony screening and filtering apparatus of the present invention includes: bacterial colony dilution agitator tank 1, level four filter jar 2, waste liquid holding vessel 3, water feeding pipeline 4, nitrogen protection pipeline 5, negative pressure air exhaust system 6, stirring lift slip table 7, filter paper change slip table 8, control system 9, fixed frame 10.
Bacterial colony dilution agitator tank 1 is fixed on fixed frame 10, and the fresh excrement and urine of qualified donor is put into it through bacterial colony dilution agitator tank 1's dog-house. The bacterial colony diluting and stirring tank 1 is of a transparent structure, can be diluted and stirred by adding water through an interface, and is filled with nitrogen to protect the activity of anaerobic bacteria.
Level four filters jar 2 and installs in 1 bottoms of bacterial colony dilution agitator tank to dilute agitator tank 1 intercommunication with the bacterial colony through the valve of 1 bottoms of bacterial colony dilution agitator tank. The valve can be controlled manually or in other ways. The four-stage filter tank 2 comprises a first-stage filter unit, a second-stage filter unit, a third-stage filter unit and a four-stage filter unit which are connected in sequence.
The waste liquid storage tank 3 is arranged at the bottom of the four-stage filter tank 2 and is communicated with the four-stage filter tank 2. Stirring lift slip table 7 is fixed on fixed frame 10 for insert or keep away from the bacterial colony with the stirring rake of carrying and dilute agitator tank 1 inside. The water adding pipeline 4 is fixed on the fixed frame 10 and is respectively communicated with the bacterial colony dilution stirring tank 1 and the four-stage filter tank 2 for supplying water. Nitrogen protection pipeline 5 is fixed on fixed frame 10, communicates bacterial colony respectively and dilutes agitator tank 1 and level four filter tank 2 for provide nitrogen gas.
The filter paper replacing sliding table 8 is fixed on the fixed frame 10 and used for controlling the replacement of the filter paper of each stage of the filter unit in the four-stage filter tank 2. The filter paper replacement sliding table 8 can drive all stages of filter units to do vertical opening and closing movement, and filter paper quick replacement is achieved.
A motor for driving the stirring paddle to rotate is arranged on the stirring lifting sliding table 7.
The negative pressure air exhaust system 6 is fixed on the fixed frame 10 and is used for vacuumizing the four-stage filter tank 2. The space pressure of each stage of filtering units is gradually reduced, and the filtering is smoother.
The control system 9 is installed on the fixed frame 10 and used for controlling the stirring lifting sliding table 7, the water feeding pipeline 4, the nitrogen protection pipeline 5, the filter paper replacement sliding table 8, the motor and the negative pressure air extraction system 6. The electric automatic control function is provided for the whole device, each movement and the automatic action of the switch component are controlled, and the whole device is more convenient to operate.
Fresh excrement of qualified donors is put into the bacterial colony dilution agitator tank 1 through the dog-house of the agitator tank, and water is added into the excrement fungus dilution agitator tank 1 through the water adding pipeline 4, and nitrogen is filled into the excrement fungus dilution agitator tank 1 through the nitrogen protection pipeline 5, and after the water addition amount is appropriate, the stirring lifting sliding table 7 drives the stirring paddle to insert into the excrement fungus dilution agitator tank 1 to implement stirring. After the stirring is finished, a valve at the bottom of the fecal bacteria dilution stirring tank 1 is opened, and the uniformly diluted fecal liquid flows into a first-stage filtering unit of a lower four-stage filtering tank 2 and is automatically filtered downwards step by step. At the moment, the negative pressure air exhaust system 6 starts to vacuumize the four-stage filter tank 2, the water adding pipeline 4 and the nitrogen protection pipeline 5 respectively add water and fill nitrogen into the four-stage filter tank 2, and the filtering is promoted to be rapidly completed together. After the filtration is finished, the sliding table 8 is replaced by filter paper, so that the interfaces of all stages of filter units can be separated, and the filter paper with the excrement residues and the collected effective thalli are taken out.
The above embodiments are provided only for illustrating the present invention and not for limiting the present invention, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention, and therefore all equivalent technical solutions should also fall within the scope of the present invention, and should be defined by the claims.
Claims (4)
1. An intestinal colony screening and filtering device, comprising:
a stationary frame (10);
the bacterial colony dilution stirring tank (1) is fixed on the fixed rack (10) and is used for placing fresh excrement;
a four-stage filter tank (2) which is arranged at the bottom of the bacterial colony diluting and stirring tank (1) and is communicated with the bacterial colony diluting and stirring tank (1) through a valve at the bottom of the bacterial colony diluting and stirring tank (1);
a waste liquid storage tank (3) which is arranged at the bottom of the four-stage filter tank (2) and is communicated with the four-stage filter tank (2);
the stirring lifting sliding table (7) is fixed on the fixed rack (10) and used for inserting or keeping away the carried stirring paddle from the colony dilution stirring tank (1);
a water adding pipeline (4) which is fixed on the fixed frame (10) and is respectively communicated with the bacterial colony dilution stirring tank (1) and the four-stage filtering tank (2) and used for providing water;
a nitrogen protection pipeline (5) which is fixed on the fixed frame (10) and is respectively communicated with the bacterial colony dilution stirring tank (1) and the four-stage filter tank (2) and used for providing nitrogen; and
and the filter paper replacing sliding table (8) is fixed on the fixed rack (10) and is used for controlling the replacement of the filter paper of each stage of filter unit in the four-stage filter tank (2).
2. The intestinal colony screening and filtering device according to claim 1, characterized in that the quaternary filtering tank (2) comprises a primary filtering unit, a secondary filtering unit, a tertiary filtering unit and a quaternary filtering unit which are connected in sequence.
3. The enteric colony filtering device of claim 1, further comprising: the motor is arranged on the stirring lifting sliding table (7) and used for driving the stirring paddle to rotate;
and the negative pressure air exhaust system (6) is fixed on the fixed rack (10) and is used for vacuumizing the four-stage filter tank (2).
4. The enteric colony filtering device of claim 3, further comprising: install on fixed frame (10), be used for control stirring lift slip table (7), water filling pipeline (4), nitrogen protection pipeline (5), filter paper change slip table (8), motor and negative pressure air exhaust system (6) control system (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110018713.0A CN112852601A (en) | 2021-01-07 | 2021-01-07 | Intestinal bacterial colony screening filtration equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110018713.0A CN112852601A (en) | 2021-01-07 | 2021-01-07 | Intestinal bacterial colony screening filtration equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112852601A true CN112852601A (en) | 2021-05-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110018713.0A Pending CN112852601A (en) | 2021-01-07 | 2021-01-07 | Intestinal bacterial colony screening filtration equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112852601A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528324A (en) * | 2021-09-16 | 2021-10-22 | 迈德(山东)科技股份有限公司 | Microorganism filtering and extracting device and filtering and extracting method |
Citations (9)
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|---|---|---|---|---|
| CN204874518U (en) * | 2015-06-25 | 2015-12-16 | 扬州市第一人民医院 | Intestinal content target composition draws equipment |
| CN105842012A (en) * | 2016-05-01 | 2016-08-10 | 上海大学 | Automatic water filtering and distributing system |
| CN206103537U (en) * | 2016-09-28 | 2017-04-19 | 西南石油大学 | Bushing type air cleaner |
| CN106929398A (en) * | 2015-12-29 | 2017-07-07 | 苏州海黄龙生物技术有限公司 | Full-automatic caprophyl separator |
| CN207507072U (en) * | 2017-08-22 | 2018-06-19 | 北京恒信未来科技开发有限公司 | A kind of petroleum transportation pipeline production coating filter |
| CN109517727A (en) * | 2019-01-17 | 2019-03-26 | 上海宝藤生物医药科技股份有限公司 | Separation device and separation method for intestinal microorganisms |
| CN208667728U (en) * | 2018-08-21 | 2019-03-29 | 卓源健康科技有限公司 | Full automatic standard intestinal flora preparation preparing instrument |
| CN211158827U (en) * | 2019-11-12 | 2020-08-04 | 上海威驰过滤***有限公司 | Filter convenient to change filter bag |
| WO2020165873A1 (en) * | 2019-02-15 | 2020-08-20 | Oncosimis Biotech Private Limited | Method and system for enhanced continuous production, secretion and separation of recombinant polypeptides from bacteria |
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2021
- 2021-01-07 CN CN202110018713.0A patent/CN112852601A/en active Pending
Patent Citations (9)
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| CN204874518U (en) * | 2015-06-25 | 2015-12-16 | 扬州市第一人民医院 | Intestinal content target composition draws equipment |
| CN106929398A (en) * | 2015-12-29 | 2017-07-07 | 苏州海黄龙生物技术有限公司 | Full-automatic caprophyl separator |
| CN105842012A (en) * | 2016-05-01 | 2016-08-10 | 上海大学 | Automatic water filtering and distributing system |
| CN206103537U (en) * | 2016-09-28 | 2017-04-19 | 西南石油大学 | Bushing type air cleaner |
| CN207507072U (en) * | 2017-08-22 | 2018-06-19 | 北京恒信未来科技开发有限公司 | A kind of petroleum transportation pipeline production coating filter |
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| WO2020165873A1 (en) * | 2019-02-15 | 2020-08-20 | Oncosimis Biotech Private Limited | Method and system for enhanced continuous production, secretion and separation of recombinant polypeptides from bacteria |
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Non-Patent Citations (1)
| Title |
|---|
| 刘庆辉: "《中国技术成果大全》", 30 November 1992, 科学技术文献出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528324A (en) * | 2021-09-16 | 2021-10-22 | 迈德(山东)科技股份有限公司 | Microorganism filtering and extracting device and filtering and extracting method |
| CN113528324B (en) * | 2021-09-16 | 2022-01-04 | 迈德(山东)科技股份有限公司 | Microorganism filtering and extracting device and filtering and extracting method |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210528 |