CN112842403A - Collection of aquatic animal breath release14CO2And method of use thereof - Google Patents
Collection of aquatic animal breath release14CO2And method of use thereof Download PDFInfo
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- CN112842403A CN112842403A CN202011547906.7A CN202011547906A CN112842403A CN 112842403 A CN112842403 A CN 112842403A CN 202011547906 A CN202011547906 A CN 202011547906A CN 112842403 A CN112842403 A CN 112842403A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B2010/0083—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements for taking gas samples
- A61B2010/0087—Breath samples
Abstract
The invention discloses a method for collecting aquatic animal breath release14CO2The device and the using method thereof comprise: a nitrogen tank, and a marking reaction bottle and a collecting bottle which are matched and connected with the nitrogen tank; one side of the top of the nitrogen tank is connected with a nitrogen filling pipe, one end of the nitrogen filling pipe is connected with an airflow collecting plate, a plurality of shunt pipes are arranged at the bottom of the airflow collecting plate in an array mode and connected to a collecting pipe, and the collecting pipe is arranged inside the marked reaction bottle; a marking reaction bottle cap is arranged at the top of the marking reaction bottle, a sample injector is arranged on one side of the marking reaction bottle cap, a first air bag is arranged on one side of the sample injector, an adjusting mechanism is arranged in the marking reaction bottle, one end of the adjusting mechanism is connected to the sample injector, and the other end of the adjusting mechanism is connected to the collecting pipe; a sample outlet is formed in the bottom of the adjusting mechanism; an air outlet is formed in the upper portion of the outer side of the marking reaction bottle, and one end of the air outlet is connected with a collecting bottle in a matched mode through a hose.
Description
Technical Field
The invention relates to a method for collecting aquatic animal breathReleasing14CO2In particular to a device for collecting aquatic animal breath release14CO2And methods of using the same.
Background
After feeding, the aquatic animals have been the focus of carbon cycle research along with activities such as absorption, excretion, respiration, secretion, etc. Aquatic animal respiration is an important process for regulating organic production (phytoplankton photosynthesis) and organic carbon consumption (respiratory consumption or remineralization) by respiring CO released by aquatic animals2Is an important component of carbon emission of the water body and is also an important source of inorganic carbon in the water body. However, conventional studies currently estimate the CO released by aquatic animals in respiration by measuring the consumption of oxygen2Amount of CO not directly released by respiration of aquatic animals2Direct measurements were performed.
At present, no respiratory release for aquatic animals has been found14CO2Collected device patents. Routine oxygen consumption method for estimating CO released by aquatic animal respiration2It is difficult to eliminate the influence of microorganisms such as bacteria, and CO produced by respiration of aquatic animals may be overestimated2Amount of the compound (A). But by radioactivity14C marking means by feeding aquatic animals14Bait of C mark, use14CO2Collecting means for directly collecting and accurately measuring the respiratory release of aquatic animals14CO2The method solves the problem that the conventional oxygen consumption estimation method can not directly measure CO2The invention aims to overcome the defects of the prior art and provide a method for directly collecting aquatic animal breath release for ecological marking experiments14CO2The apparatus of (1).
Disclosure of Invention
The invention overcomes the defects of the prior art and provides a device for collecting aquatic animal breath release14CO2And methods of using the same.
In order to achieve the purpose, the invention adopts the technical scheme that: collection of aquatic animal breath release14CO2The apparatus of (1), comprising: nitrogen tank and mark matched and connected with nitrogen tankA reaction bottle and a collecting bottle, which are characterized in that,
a nitrogen filling pipe is arranged on one side of the top of the nitrogen tank, one end of the nitrogen filling pipe is connected with an airflow collecting plate, a plurality of flow dividing pipes are arranged at the bottom of the airflow collecting plate in an array mode and connected to a collecting pipe, and the collecting pipe is arranged inside the marked reaction bottle;
a marking reaction bottle cap is arranged at the top of the marking reaction bottle, a sample injector is arranged on one side of the marking reaction bottle cap, a first air bag is arranged on one side of the sample injector, an adjusting mechanism is arranged in the marking reaction bottle, one end of the adjusting mechanism is connected to the sample injector, and the other end of the adjusting mechanism is connected to the collecting pipe; a sample outlet is formed in the bottom of the adjusting mechanism;
an air outlet is formed in the upper portion of the outer side of the marking reaction bottle, and one end of the air outlet is connected with a collecting bottle in a matched mode through a hose.
In a preferred embodiment of the present invention, the shunt regulating valve is provided with a plurality of regulating switches, the plurality of regulating switches can be adjusted in a linkage manner, and the regulating switches can regulate the opening degree.
In a preferred embodiment of the invention, one side of the marking reaction bottle is provided with a regulating tube, a second air bag is arranged in the marking reaction bottle, and the regulating tube is communicated with the second air bag.
In a preferred embodiment of the invention, a collecting bottle cap is arranged on the top of the collecting bottle, and a plurality of vent holes are arranged on the collecting bottle cap at intervals.
In a preferred embodiment of the present invention, one end of the flexible tube penetrates through the bottle cap of the collection bottle and is inserted into the bottom of the collection bottle, and one end of the flexible tube located inside the collection bottle is of a hook-shaped structure.
In a preferred embodiment of the invention, a pressure reducing valve is arranged at the top of the nitrogen tank, and a nitrogen tank switch is arranged between the nitrogen tank and the nitrogen filling pipe.
In a preferred embodiment of the invention, a plurality of universal wheels are arranged at the bottom of the nitrogen tank along the circumferential direction.
To achieve the above objectThe second technical scheme adopted by the invention is as follows: collection of aquatic animal breath release14CO2The use method of the device comprises the following steps:
before collection, a pressure reducing valve and an adjusting switch are adjusted to ensure that nitrogen is slowly filled into the labeled reaction bottle at a constant speed;
during collection, sodium hydroxide solution is injected into the collection bottle and is controlled by the sample injector to enter the labeled reaction bottle14CO3 2-And H14CO3 -Then, an appropriate amount of dilute hydrochloric acid was added to the labeled reaction flask via a sample injector, and the sample injector was immediately sealed with a first balloon. Opening a nitrogen tank switch to enable nitrogen to be slowly filled into the bottom of the water sample in the labeled reaction bottle at a constant speed, and enabling the generated water sample to be14CO2Driving the mixture into a collecting bottle to be absorbed by sodium hydroxide solution;
by squeezing the first air bag at the mouth of the sample injector, the air bag will remain in the sample injector14CO2Driving to a collecting bottle; by squeezing a second bladder inside the labelling reaction, the nitrogen gas charge can be assisted to produce the product in the labelling reaction flask14CO2Driving the mixture into a collecting bottle for collection.
In a preferred embodiment of the invention, the concentration of sodium hydroxide in the collection bottle is 2mol/L, and the volume is 5 mL.
In a preferred embodiment of the present invention, the labeled reaction bottle and the collection bottle are made of glass.
The invention solves the defects in the background technology, and has the following beneficial effects:
(1) directly collectable for determining respiratory release in aquatic animals14CO2Solves the problem that the conventional oxygen consumption estimation method can not directly measure CO2The measurement precision is improved due to the difficult problem of quantity;
(2) the injection of dilute hydrochloric acid is convenient, and can prevent the sample from being polluted14CO2The loss is avoided, the disassembly is convenient, and the reutilization can be realized;
(3) the sample inlet is connected with a second air bag which not only can play a role of sealing the sample inlet, but also can remain on the sample inletIn the sample injector14CO2Driving to a collecting bottle; first bladder compression may be increased in conjunction with nitrogen inflation14CO2The collection efficiency of (a); .
(4) One side in the marking reaction bottle is provided with a second air bag, the size of the second air bag is changed by adjusting the air quantity in the second air bag, the inner space of the marking reaction bottle can be extruded by the way, and the air generated in the marking reaction bottle can be marked14CO2Fully drives into the collecting bottle and improves14CO2Collection efficiency, control is simple nimble.
Drawings
The invention is further explained below with reference to the figures and examples;
FIG. 1 is a partial perspective block diagram of a preferred embodiment of the present invention;
FIG. 2 is a schematic view of a partial connection structure of a labeled reaction flask and a collection flask according to a preferred embodiment of the present invention;
FIG. 3 is a schematic diagram of the internal structure of the labeled reaction flask and the collection flask according to the preferred embodiment of the present invention;
fig. 4 is a schematic view of a shunt tube distribution according to a preferred embodiment of the present invention;
FIG. 5 is a schematic view of the vent hole distribution of the preferred embodiment of the present invention;
in the figure: 1. a nitrogen tank; 2. an adjustment switch; 3. a first air bag; 4. a universal wheel; 5. a pressure reducing valve; 6. the device comprises a nitrogen tank switch, 7, a nitrogen filling pipe, 8, a marking reaction bottle, 9, a marking reaction bottle cap, 10, a sample injector, 11, a flow dividing pipe, 12, an airflow collecting plate, 13, a regulating pipe, 14, an air outlet, 15, a collecting bottle, 16, a gas collecting pipe, 17, a regulating mechanism, 18, a collecting bottle cap, 19, a sample outlet, 20, a second air bag, 21, a collecting pipe, 22 and a vent hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be taken as limiting the scope of the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, the meaning of "a plurality" is two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations. As shown in FIGS. 1-4, the present invention discloses collecting aquatic animal breath release14CO2In a deviceSchematic view of a partial three-dimensional structure of (a);
in particular, a method for collecting aquatic animal breath release14CO2The apparatus of (1), comprising: a nitrogen tank 1, and a marking reaction bottle 8 and a collecting bottle 15 which are matched and connected with the nitrogen tank 1;
one side of the top of the nitrogen tank 1 is connected with a nitrogen filling pipe 7, one end of the nitrogen filling pipe 7 is connected with an airflow collecting plate 12, a plurality of shunt tubes 11 are arranged at the bottom of the airflow collecting plate 12 in an array mode, the shunt tubes 11 are connected to a collecting pipe 21, and the collecting pipe 21 is arranged inside the marking reaction bottle 8;
a marking reaction bottle cap 9 is arranged at the top of the marking reaction bottle 8, a sample injector 10 is arranged on one side of the marking reaction bottle cap 9, a first air bag 3 is arranged on one side of the sample injector 10, an adjusting mechanism 17 is arranged inside the marking reaction bottle 8, one end of the adjusting mechanism 17 is connected to the sample injector 10, and the other end of the adjusting mechanism 17 is connected to a collecting pipe 21;
the bottom of the adjusting mechanism 17 is provided with a sample outlet 19;
the upper part of the outer side of the marking reaction bottle 8 is provided with a gas outlet 14, and one end of the gas outlet 14 is matched with a gas collecting pipe 16 and is connected with a collecting bottle 15.
It should be noted that the pressure reducing valve 5 can reduce the pressure of the high-purity nitrogen, and the nitrogen gas filling pipe 7 and the gas collecting pipe 16 are both transparent hoses and also channels for gas input and output; the airflow collecting plate 12 is made of stainless steel, the marking reaction bottles 8 and the collecting bottles 15 are made of glass, and the marking reaction bottles 8 and the collecting bottles 15 with the capacities of 30mL and 20mL are selected; the bottle cap 9 of the marked reaction bottle is a perforated screw cap and contains an ultra-low-loss PTFE/silica gel spacer, and the spacer and the perforated screw cap are tightly adhered by glue to prevent air leakage; the collecting bottle cap 18 is a hollow screw cap, and a plurality of vent holes are formed in the top of the collecting bottle cap 18 in an array mode.
According to the embodiment of the invention, the adjusting switches 2 are arranged on any shunt tube 11, the adjusting switches 2 on the shunt tubes 11 can be adjusted in a linkage manner, and the opening degree of the adjusting switches 2 can be adjusted.
The first bladder 3 is connected to the sample injector 10, and not only seals the sample injector port, but also seals the remaining sample in the sample injector14CO2Driving into the collecting bottle to avoid14CO2The collection is incomplete, and the control is simple and flexible.
It can be understood that there are 6 shunt tubes 11, there are 6 regulating switches on the air flow collecting plate, each regulating switch controls one shunt tube correspondingly, in the nitrogen filling process, 1 shunt tube can be controlled to be opened, and the other 5 shunt tubes can be controlled to be closed, or 2, 3, 4, 5 or 6 shunt tubes can be controlled to be opened, and the opening degree of the regulating switches can be regulated to control the air flow in the shunt tubes.
As shown in FIG. 3, the present invention discloses a schematic view of the internal structure of a labeled reaction flask 8;
according to the embodiment of the invention, the adjusting pipe 13 is arranged on one side of the marking reaction bottle 8, the second air bag 20 is arranged in the marking reaction bottle 8, and the adjusting pipe 13 is communicated with the second air bag 20.
The second air cell 20 is provided at one side in the marking reaction flask 8, and the size of the second air cell 20 is changed by adjusting the amount of air in the second air cell 20, so that the inner space of the marking reaction flask 8 can be squeezed to mark the gas generated in the reaction flask14CO2Fully drives into the collecting bottle and improves14CO2Collection efficiency, control is simple nimble.
According to the embodiment of the invention, the top of the nitrogen tank 1 is provided with a pressure reducing valve 5, and a nitrogen tank switch 6 is arranged between the nitrogen tank 1 and the nitrogen filling pipe 7.
According to the embodiment of the invention, a collecting bottle cap 18 is arranged on the top of the collecting bottle 15, and a plurality of vent holes 22 are arranged on the collecting bottle cap 18 at intervals.
In a preferred embodiment of the present invention, one end of the gas collection tube 16 penetrates through the bottle cap 18 of the collection bottle and is inserted into the bottom of the collection bottle 15, and one end of the hose inside the collection bottle 15 is of a hook-shaped structure.
According to the embodiment of the invention, the bottom of the nitrogen tank 1 is provided with a plurality of universal wheels 4 along the circumferential direction.
In order to achieve the above purpose, the second technical solution adopted by the present invention is: collection of aquatic animal breath release14CO2The use method of the device comprises the following steps:
before collection, the pressure reducing valve 5 and the regulating switch 2 are regulated to ensure that nitrogen is slowly filled into the labeled reaction bottle at a constant speed;
during collection, sodium hydroxide solution is injected into the collection bottle 15 and is controlled by the sample injector 10 to enter the labeled reaction bottle 814CO3 2-And H14CO3 -Then adding a proper amount of dilute hydrochloric acid into the marking reaction bottle through the sample injector 10, and immediately sealing the sample injector 10 by using the first air bag 3;
opening a nitrogen tank switch 6 to ensure that nitrogen is slowly filled into the bottom of a water sample in a labeled reaction bottle 8 at a constant speed, and generating14CO2Driving to a collecting bottle 15 to be absorbed by sodium hydroxide solution;
the first air bag 3 at the opening of the sample injector 10 is pressed, and the air bag will remain in the sample injector 1014CO2Driving into a collection bottle 15; the size of the second air bag 20 in the marking reaction bottle 8 is adjusted through the adjusting pipe 13 to assist the nitrogen gas to inflate the generated air in the marking reaction bottle 814CO2Driving the mixture into a collecting bottle 15 for collection.
It should be noted that the end of the sample outlet is located below the liquid level of the water sample inside the labeled reaction bottle, and the distance from the sample outlet to the bottom end inside the labeled reaction bottle is 1-5mm, preferably 3 mm.
According to the embodiment of the present invention, the concentration of sodium hydroxide in the collection bottle 15 is 2mol/L, and the volume is 5 mL.
According to the embodiment of the invention, the labeling reaction flask 8 and the collection flask 15 are made of glass.
It should be noted that the air outlet 14 is connected to the top of the collecting bottle 15 through the gas collecting pipe 16, the pressure reducing valve 5 can reduce the pressure of high-purity nitrogen, and the nitrogen gas filling pipe 7 and the gas collecting pipe 16 are both transparent hoses and also are channels for gas input and output; the airflow collecting plate 12 is made of stainless steel, the marking reaction bottles 8 and the collecting bottles 15 are made of glass, and the marking reaction bottles 8 and the collecting bottles 15 with the capacities of 30mL and 20mL are selected; the bottle cap 9 of the marked reaction bottle is a perforated screw cap and contains an ultra-low-loss PTFE/silica gel spacer, and the spacer and the perforated screw cap are tightly adhered by glue to prevent air leakage; as shown in FIG. 5, the invention discloses a schematic view of vent hole distribution, wherein a collecting bottle cap 18 is a hollow screw cap and is provided with a plurality of vent holes.
In a preferred embodiment of the present invention, one end of the gas collection tube 16 penetrates through the bottle cap 18 of the collection bottle and is inserted into the bottom of the collection bottle 15, and one end of the hose inside the collection bottle 15 is of a hook-shaped structure.
It should be noted that one end of the hose is located below the liquid level of the sodium hydroxide solution in the collection bottle, the end of the hook-shaped structure of the hose faces upward, the distance between the end of the hose and the bottom end inside the collection bottle is 1-5mm, and the preferred distance between the hose and the bottom end inside the collection bottle is 2 mm.
According to the embodiment of the invention, the adjusting switches 2 are arranged on any shunt tube 11, the adjusting switches 2 on the shunt tubes 11 can be adjusted in a linkage manner, and the opening degree of the adjusting switches 2 can be adjusted.
The first bladder 3 is connected to the sample injector 10, and not only seals the sample injector port, but also seals the remaining sample in the sample injector14CO2Driving into the collecting bottle 15 to avoid14CO2The collection is incomplete, and the control is simple and flexible.
It can be understood that there are 6 shunt tubes 11, there are 6 regulating switches 2 on the air flow collecting plate 12, each regulating switch controls one shunt tube correspondingly, in the nitrogen filling process, 1 shunt tube can be controlled to be opened, the other 5 shunt tubes can be controlled to be closed, or 2, 3, 4, 5 or 6 shunt tubes can be controlled to be opened, and the opening degree of the regulating switches can be adjusted to control the air flow in the shunt tubes.
According to the embodiment of the invention, the adjusting tube 13 is arranged on one side of the marking reaction bottle 8, the second air bag 20 is arranged in the marking reaction bottle 8, and the second air bag adjusting tube 13 is communicated with the second air bag 20.
The second airbag 20 is provided on one side of the inside of the marker reaction flask 8, and the second airbag 2 is adjusted0 internal gas amount, the size of the second gas bag 20 is changed, the internal space of the marking reaction bottle 8 can be extruded by the way, and the gas generated in the marking reaction bottle can be marked14CO2Fully drives into the collecting bottle 15 and improves14CO2Collection efficiency, control is simple nimble.
According to the embodiment of the invention, the top of the nitrogen tank 1 is provided with a pressure reducing valve 5, and a nitrogen tank switch 6 is arranged between the nitrogen tank 1 and the nitrogen filling pipe 7.
In conclusion, the respiratory release of aquatic animals can be directly measured14CO2Solves the problem that the conventional oxygen consumption estimation method can not directly measure CO2The measurement precision is improved due to the difficult problem of quantity;
the injection of hydrochloric acid is convenient, and can prevent the sample from being contaminated14CO2The running-off of (2) is convenient to disassemble and can be repeatedly used.
The second air bag 20 is arranged at one side in the marking reaction bottle 8, the size of the second air bag 20 is changed by adjusting the air quantity in the second air bag 20, the inner space of the marking reaction bottle 8 can be extruded by the way, and the air generated in the marking reaction bottle 8 can be marked14CO2Fully drives into the collecting bottle 15 and improves14CO2Collection efficiency, control is simple nimble.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (10)
1. Collection of aquatic animal breath release14CO2The apparatus of (1), comprising: a nitrogen tank, a marking reaction bottle and a collecting bottle which are matched and connected with the nitrogen tank, and is characterized in that,
one side of the top of the nitrogen tank is connected with a nitrogen filling pipe, one end of the nitrogen filling pipe is connected with an airflow collecting plate, a plurality of shunt pipes are arranged at the bottom of the airflow collecting plate in an array mode and connected to a collecting pipe, and the collecting pipe is arranged inside the marked reaction bottle;
a marking reaction bottle cap is arranged at the top of the marking reaction bottle, a sample injector is arranged on one side of the marking reaction bottle cap, a first air bag is arranged on one side of the sample injector, an adjusting mechanism is arranged in the marking reaction bottle, one end of the adjusting mechanism is connected to the sample injector, and the other end of the adjusting mechanism is connected to the collecting pipe;
a sample outlet is formed in the bottom of the adjusting mechanism;
an air outlet is formed in the upper portion of the outer side of the marking reaction bottle, and one end of the air outlet is connected with a collecting bottle in a matched mode through a hose.
2. The method of claim 1, wherein the step of collecting the respiratory release comprises collecting the respiratory release from an aquatic animal14CO2The device of (2), its characterized in that, be provided with a plurality of regulating switch on the reposition of redundant personnel governing valve, a plurality of regulating switch can the linkage regulation, regulating switch can adjust the aperture.
3. The method of claim 1, wherein the step of collecting the respiratory release comprises collecting the respiratory release from an aquatic animal14CO2The device is characterized in that one side of the marking reaction bottle is provided with an adjusting pipe, a second air bag is arranged in the marking reaction bottle, and the adjusting pipe is communicated with the second air bag.
4. The method of claim 1, wherein the step of collecting the respiratory release comprises collecting the respiratory release from an aquatic animal14CO2The device is characterized in that a collecting bottle cap is arranged at the top of the collecting bottle, and a plurality of vent holes are formed in the collecting bottle cap at intervals.
5. The method of claim 1, wherein the step of collecting the respiratory release comprises collecting the respiratory release from an aquatic animal14CO2The device is characterized in that one end of the hose penetrates through the bottle cap of the collecting bottle and is inserted into the bottom of the collecting bottle, and one end of the hose positioned in the collecting bottle is of a hook-shaped structure.
6. The method of claim 1, wherein the step of collecting the respiratory release comprises collecting the respiratory release from an aquatic animal14CO2The device is characterized in that a pressure reducing valve is arranged at the top of the nitrogen tank, and a nitrogen tank switch is arranged between the nitrogen tank and the nitrogen filling pipe.
7. The method of claim 1, wherein the step of collecting the respiratory release comprises collecting the respiratory release from an aquatic animal14CO2The device is characterized in that a plurality of universal wheels are arranged at the bottom of the nitrogen tank along the circumferential direction.
8. A method of collecting aquatic animal breath release as claimed in any one of claims 1 to 714CO2The method for using the device is characterized by comprising the following steps:
before collection, a pressure reducing valve and an adjusting switch are adjusted to ensure that nitrogen is slowly filled into the labeled reaction bottle at a constant speed;
during collection, sodium hydroxide solution is injected into the collection bottle and is controlled by the sample injector to enter the labeled reaction bottle14CO3 2-And H14CO3 -Adding a proper amount of dilute hydrochloric acid into the marking reaction bottle through a sample injector, and immediately sealing the sample injector by using a first air bag;
opening a nitrogen tank switch to enable nitrogen to be slowly filled into the bottom of the water sample in the labeled reaction bottle at a constant speed, and enabling the generated water sample to be14CO2Driving the mixture into a collecting bottle to be absorbed by sodium hydroxide solution;
by squeezing the first air bag at the mouth of the sample injector, the air bag will remain in the sample injector14CO2Driving to a collecting bottle; adjusting the gas flow of the second gas bag inside the labeled reaction flask to assist the nitrogen gas to inflate the gas generated in the labeled reaction flask14CO2Driving the mixture into a collecting bottle for collection.
9. A method of collecting aquatic animal breath release according to claim 814CO2The use of the device of (1), characterized in thatThe concentration of sodium hydroxide in the collection bottle was 2mol/L, and the volume was 5 mL.
10. A method of collecting aquatic animal breath release according to claim 814CO2The use method of the device is characterized in that the marking reaction bottle and the collecting bottle are made of glass materials.
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Cited By (1)
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