CN112831419A - Nucleic acid sample save set - Google Patents
Nucleic acid sample save set Download PDFInfo
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- CN112831419A CN112831419A CN202110084150.5A CN202110084150A CN112831419A CN 112831419 A CN112831419 A CN 112831419A CN 202110084150 A CN202110084150 A CN 202110084150A CN 112831419 A CN112831419 A CN 112831419A
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- refrigeration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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Abstract
The invention discloses a nucleic acid sample storage device, and belongs to the technical field of sample storage. The nucleic acid sample preservation device comprises a workbench, a first refrigeration seat, a second refrigeration seat, a first refrigeration assembly and a second refrigeration assembly. The first refrigeration seat is arranged on the workbench and used for placing the 96 pore plate, and 96 pits corresponding to the bottom of the 96 pore plate are arranged on the first refrigeration seat; the second refrigerating seat is arranged on the workbench and arranged at the same side as the first refrigerating seat, and can be used for placing an eight-joint pipe and a UP pipe at the same time; the first refrigeration assembly and the second refrigeration assembly are arranged on one side, deviating from the first refrigeration seat and the second refrigeration seat, of the workbench, the first refrigeration assembly is arranged corresponding to the first refrigeration seat, and the second refrigeration assembly is arranged corresponding to the second refrigeration seat. The invention can be used for placing sample containers with different specifications, has wide application range and good low-temperature preservation effect.
Description
Technical Field
The invention relates to the technical field of sample preservation, in particular to a nucleic acid sample preservation device.
Background
The liquid treatment station is an automatic liquid-transferring working platform with flexible function and excellent performance, and a high-throughput biomedical working station with powerful function can meet various liquid treatment works such as liquid transferring, liquid separation, dilution, mixing and the like in a laboratory, is suitable for a full-automatic extraction and purification system for separating DNA/RNA, protein and cells, and is designed for solving the current and future extraction and purification problems of customers.
Preservation of a cryogenic sample nucleic acid is required during processing of the sample using a liquid processing station. The existing low-temperature nucleic acid sample storage device has poor storage effect and poor universality.
Disclosure of Invention
The invention aims to provide a low-temperature nucleic acid sample storage device which is good in storage effect and strong in applicability.
In order to realize the purpose, the following technical scheme is provided:
a nucleic acid sample preservation device, comprising:
a work table;
the first refrigerating seat is arranged on the workbench and used for placing a 96-hole plate, and 96 pits corresponding to the bottom of the 96-hole plate are formed in the first refrigerating seat;
the second refrigerating seat is arranged on the workbench and is arranged at the same side of the first refrigerating seat, and the eight-joint pipe and the UP pipe can be placed on the second refrigerating seat at the same time;
the workbench is arranged on the back of the workbench, the first refrigeration seat and one side of the second refrigeration seat are arranged, the first refrigeration component corresponds to the first refrigeration seat, and the second refrigeration component corresponds to the second refrigeration seat.
In a preferred embodiment of the nucleic acid sample storage device of the present invention, a pillar is provided between each of two adjacent wells.
In a preferred embodiment of the nucleic acid sample storage device of the present invention, the second cooling stage has at least one octal-gang well group for placing the octal-gang tubes and at least one containing well for placing the UP tubes.
As a preferable embodiment of the nucleic acid sample storage device of the present invention, the second refrigeration seat further includes a liner tube, the liner tube may be placed in the accommodation hole, the UP tube may be placed in the liner tube, the liner tube is located between the UP tube and an inner wall of the accommodation hole, and the liner tube includes an adapter portion extending into the accommodation hole and a placing portion placed on an opening of the accommodation hole.
As a preferable scheme of the nucleic acid sample storage device of the present invention, the first refrigeration assembly includes a first refrigeration sheet, a first radiator, and a first fan that are sequentially disposed, and the second refrigeration assembly includes a second refrigeration sheet, a second radiator, and a second fan that are sequentially disposed.
As a preferable scheme of the nucleic acid sample storage device, a groove is formed in the workbench, the first refrigeration sheet is arranged at the bottom of the groove, and the first refrigeration seat is placed in the groove.
In a preferred embodiment of the nucleic acid sample storage device of the present invention, a conductive plate is formed by extending one side of the second cooling seat, and the second cooling plate is disposed in close contact with the conductive plate.
The nucleic acid sample storage apparatus according to the present invention preferably further comprises:
the first temperature measuring assembly is used for measuring the temperature of the first refrigerating seat in real time;
and the second temperature measuring component is used for measuring the temperature of the second refrigerating seat in real time.
The nucleic acid sample storage apparatus according to the present invention preferably further comprises:
the first regulator is used for regulating the power of the first refrigerating sheet;
and the second regulator is used for regulating the power of the second refrigerating sheet.
The invention has the beneficial effects that:
according to the nucleic acid sample preservation device provided by the invention, the table board of the workbench is provided with the first refrigerating seat and the second refrigerating seat, and the side of the workbench, which is far away from the first refrigerating seat and the second refrigerating seat, is provided with the first refrigerating assembly and the second refrigerating assembly. The first refrigeration seat is used for placing the 96 pore plate, and 96 pits corresponding to the bottom of the 96 pore plate are arranged on the first refrigeration seat, so that the stable placement of the 96 pore plate is ensured; the second refrigeration seat can be used for placing an eight-connection pipe and an UP pipe at the same time; make this nucleic acid sample save set can place the sample container of different specifications, application scope is wide. First refrigeration subassembly cools off first refrigeration seat, and second refrigeration subassembly cools off the second refrigeration seat for this nucleic acid sample save set's low temperature is preserved effectually, guarantees the final performance of sample.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments of the present invention will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the contents of the embodiments of the present invention and the drawings without creative efforts.
FIG. 1 is a first isometric view of a nucleic acid sample retention device provided in accordance with an embodiment of the invention;
FIG. 2 is an exploded view of a nucleic acid sample storage device according to an embodiment of the present invention;
FIG. 3 is a second isometric view of a nucleic acid sample retention device provided in accordance with an embodiment of the invention.
Reference numerals:
10-96 well plates;
1-a workbench; 2-a first refrigeration seat; 3-a second refrigeration seat; 4-a first refrigeration component; 5-a second refrigeration component; 6-a first temperature measuring assembly; 7-a second temperature measuring assembly;
11-a groove; 12-mounting a frame;
21-pits; 22-a pillar;
31-octal groups of holes; 32-a containment hole; 33-a liner tube; 34-a conductive plate;
331-an adaptation portion; 332-placing section;
41-a first refrigeration sheet; 42-a first heat sink; 43-a first fan;
51-a second chilling plate; 52-a second heat sink; 53-second fan.
Detailed Description
In order to make the technical problems solved, technical solutions adopted and technical effects achieved by the present invention clearer, the technical solutions of the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.
In the description of the present invention, it should be noted that unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in FIGS. 1 to 3, this example provides a nucleic acid sample storage apparatus, which is a part of a liquid processing station, for cryopreserving a low-temperature nucleic acid sample. The nucleic acid sample storage device comprises a workbench 1, a first refrigerating seat 2 and a second refrigerating seat 3 which are arranged on the surface of the workbench 1, and a first refrigerating component 4 and a second refrigerating component 5 which are arranged on one side of the workbench 1 far away from the first refrigerating seat 2 and the second refrigerating seat 3. The first refrigerating seat 2 is used for placing the 96-pore plate 10, the second refrigerating seat 3 is arranged at the same side of the first refrigerating seat 2 and can be used for placing an eight-union-pipe and a UP pipe simultaneously, so that the nucleic acid sample preservation device can be used for placing sample containers with different specifications, and the application range is wide. The first refrigerating assembly 4 is arranged corresponding to the first refrigerating seat 2 and used for cooling the first refrigerating seat 2; the second refrigerating assembly 5 is arranged corresponding to the second refrigerating seat 3 and used for cooling the second refrigerating seat 3; the low-temperature preservation effect is good, and the final performance of the sample is ensured.
Wherein, be equipped with on the first refrigeration seat 2 with 96 pit 21 corresponding with 96 orifice plate 10 bottoms, guarantee that 96 orifice plate 10 stably places. Preferably, the pillars 22 are disposed between two adjacent concave pits 21, so as to further improve the placing stability and the convenience of the placing operation of the 96-well plate 10.
The second refrigerating seat 3 is provided with at least one octal hole group 31 and at least one accommodating hole 32, the octal hole group 31 is used for accommodating octal tubes, the accommodating hole 32 is used for accommodating UP tubes, and the two different consumables of the octal tubes and the UP tubes can be compatible to cool simultaneously.
Preferably, the second refrigeration seat 3 also comprises a liner 33. The liner 33 can be placed inside the receiving hole 32 with the UP tube placed in the liner 33 and the liner 33 between the UP tube and the inner wall of the receiving hole 32. The liner tube 33 includes an adapter portion 331 extending into the receiving hole 32 and a landing portion 332 landing on the opening of the receiving hole 32, and the UP tube extends into the adapter portion 331. By selecting the adapting parts 331 with different sizes, the UP pipe adapting device can adapt to UP pipes with different specifications, and the universality of the device is further improved.
Further, referring to fig. 3, the first cooling assembly 4 includes a first cooling sheet 41, a first radiator 42 and a first fan 43, which are sequentially arranged, and the second cooling assembly 5 includes a second cooling sheet 51, a second radiator 52 and a second fan 53, which are sequentially arranged. First refrigeration piece 41 is close to first refrigeration seat 2 and sets up, and first refrigeration piece 41 carries out heat-conduction with first refrigeration seat 2, and first radiator 42 carries out heat-conduction with first refrigeration piece 41, and first fan 43 accelerates the heat-conduction of first radiator 42 and air, guarantees that the temperature of first refrigeration seat 2 is in required low temperature all the time. Similarly, the second cooling plate 51 is disposed close to the second cooling seat 3, the second cooling plate 51 conducts heat with the second cooling seat 3, the second radiator 52 conducts heat with the second cooling plate 51, and the second fan 53 accelerates heat conduction between the second radiator 52 and the air, so as to ensure that the temperature of the second cooling seat 3 is always at the required low temperature.
The first refrigerating sheet 41 and the second refrigerating sheet 51 are preferably semiconductor refrigerating sheets, and have the advantages of no noise, no vibration, no need of refrigerant, small volume, light weight and the like.
Be equipped with recess 11 on the mesa of workstation 1, the tank bottom of recess 11 is located to first refrigeration piece 41, first refrigeration seat 2 is placed in recess 11, guarantee the stable of first refrigeration seat 2 and place, and through locating the tank bottom of recess 11 with first refrigeration piece 41, reduce the distance between first refrigeration piece 41 and the first refrigeration seat 2, make the heat of sample transmit first refrigeration piece 41 more high-efficiently, realize more efficient cooling and more real-time temperature control.
One side of the table-board of the working table 1 is provided with a mounting frame 12, and the second refrigerating seat 3 is placed in the mounting frame 12. One side of second refrigeration seat 3 extends and forms conduction board 34, and conduction board 34 runs through workstation 1, and the vertical setting of second refrigeration piece 51 is hugged closely conduction board 34 and is set up, and second radiator 52 and second fan 53 also keep vertical setting with second refrigeration piece 51. Through with the vertical setting of whole second refrigeration subassembly 5, above-mentioned whole 4 levels of first refrigeration subassembly set up, can utilize the space of work bottom better, prevent that second refrigeration subassembly 5 from interfering with first refrigeration subassembly 4.
Further, the nucleic acid sample storage apparatus further includes a first temperature measuring unit 6 and a second temperature measuring unit 7. The first temperature measuring assembly 6 is used for measuring the temperature of the first refrigerating seat 2 in real time, and the second temperature measuring assembly 7 is used for measuring the temperature of the second refrigerating seat 3 in real time. The first temperature measurement assembly 6 and the second temperature measurement assembly 7 are both temperature sensors, materials are convenient to obtain, and measurement is accurate.
The nucleic acid sample storage device further comprises a first regulator and a second regulator. The first regulator regulates the power of the first refrigerating sheet 41 in real time according to the temperature value measured by the first temperature measuring component 6; the second regulator regulates the power of the second refrigerating sheet 51 in real time according to the temperature value measured by the second temperature measuring component 7; and then the temperature of the first refrigerating seat 2 and the second refrigerating seat 3 is adjusted in real time. The first regulator and the second regulator can control the input current of the first cooling plate 41 and the second cooling plate 51 to regulate the power of the first cooling plate 41 and the second cooling plate 51.
The embodiment also provides a liquid processing station, which comprises the nucleic acid sample storage device, can be used for placing sample containers with different specifications, and has strong adaptability and good cooling effect.
It is to be noted that the foregoing is only illustrative of the preferred embodiments of the present invention and the technical principles employed. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail by the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the present invention, and the scope of the present invention is determined by the scope of the appended claims.
Claims (9)
1. A nucleic acid sample storage device, comprising:
a table (1);
the first refrigerating seat (2) is arranged on the workbench (1), the first refrigerating seat (2) is used for placing a 96-hole plate (10), and 96 pits (21) corresponding to the bottom of the 96-hole plate (10) are formed in the first refrigerating seat (2);
the second refrigerating seat (3) is arranged on the workbench (1) and arranged on the same side as the first refrigerating seat (2), and the eight-joint pipe and the UP pipe can be placed on the second refrigerating seat (3) at the same time;
first refrigeration subassembly (4) and second refrigeration subassembly (5) are located workstation (1) deviates from first refrigeration seat (2) with one side of second refrigeration seat (3), first refrigeration subassembly (4) with first refrigeration seat (2) correspond the setting, second refrigeration subassembly (5) with second refrigeration seat (3) correspond the setting.
2. The nucleic acid sample storage device according to claim 1, wherein a pillar (22) is provided between each of two adjacent wells (21).
3. The nucleic acid sample storage device according to claim 1, wherein the second cooling block (3) is provided with at least one octal-UP hole set (31) and at least one housing hole (32), the octal-UP hole set (31) is used for placing the octal-UP tube, and the housing hole (32) is used for placing the UP tube.
4. The nucleic acid sample storage device according to claim 3, wherein the second cooling seat (3) further comprises a liner tube (33), the liner tube (33) is placeable in the housing hole (32), the UP tube is placeable in the liner tube (33), the liner tube (33) is located between the UP tube and the inner wall of the housing hole (32), and the liner tube (33) comprises an adapter portion (331) extending into the housing hole (32) and a landing portion (332) landing on the opening of the housing hole (32).
5. The nucleic acid sample storage device according to any one of claims 1 to 4, wherein the first refrigeration assembly (4) comprises a first refrigeration sheet (41), a first heat sink (42) and a first fan (43) which are arranged in sequence, and the second refrigeration assembly (5) comprises a second refrigeration sheet (51), a second heat sink (52) and a second fan (53) which are arranged in sequence.
6. The nucleic acid sample preservation device according to claim 5, characterized in that the workbench (1) is provided with a groove (11), the first refrigeration sheet (41) is arranged at the bottom of the groove (11), and the first refrigeration seat (2) is placed in the groove (11).
7. The nucleic acid sample storage device according to claim 5, wherein one side of the second refrigeration seat (3) extends to form a conductive plate (34), and the second refrigeration sheet (51) is arranged to be close to the conductive plate (34).
8. The nucleic acid sample storage device according to claim 5, further comprising:
a first temperature measuring assembly (6) for measuring the temperature of the first refrigeration seat (2) in real time;
and the second temperature measuring component (7) is used for measuring the temperature of the second refrigerating seat (3) in real time.
9. The nucleic acid sample storage device according to claim 5, further comprising:
a first regulator for regulating the power of said first refrigeration pill (41);
a second regulator for regulating the power of the second cooling plate (51).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110084150.5A CN112831419A (en) | 2021-01-21 | 2021-01-21 | Nucleic acid sample save set |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110084150.5A CN112831419A (en) | 2021-01-21 | 2021-01-21 | Nucleic acid sample save set |
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| Publication Number | Publication Date |
|---|---|
| CN112831419A true CN112831419A (en) | 2021-05-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110084150.5A Pending CN112831419A (en) | 2021-01-21 | 2021-01-21 | Nucleic acid sample save set |
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| CN (1) | CN112831419A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106938205A (en) * | 2017-05-10 | 2017-07-11 | 贵州大学 | A kind of multifunctional low-temperature orifice fitting |
| CN208373147U (en) * | 2018-05-03 | 2019-01-15 | 成都中创清科医学检验所有限公司 | A kind of low temperature EP pipe support for PCR experiment |
| CN208516102U (en) * | 2018-07-12 | 2019-02-19 | 广州高盛智造科技有限公司 | A kind of sample save set |
| CN210613793U (en) * | 2019-08-15 | 2020-05-26 | 东莞兰卫医学检验实验室有限公司 | EP manages rack combination |
| CN211142057U (en) * | 2019-11-11 | 2020-07-31 | 中国人民公安大学 | DNA sample case for criminal investigation |
| CN214881597U (en) * | 2021-01-21 | 2021-11-26 | 广州高盛智造科技有限公司 | Nucleic acid sample save set |
-
2021
- 2021-01-21 CN CN202110084150.5A patent/CN112831419A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106938205A (en) * | 2017-05-10 | 2017-07-11 | 贵州大学 | A kind of multifunctional low-temperature orifice fitting |
| CN208373147U (en) * | 2018-05-03 | 2019-01-15 | 成都中创清科医学检验所有限公司 | A kind of low temperature EP pipe support for PCR experiment |
| CN208516102U (en) * | 2018-07-12 | 2019-02-19 | 广州高盛智造科技有限公司 | A kind of sample save set |
| CN210613793U (en) * | 2019-08-15 | 2020-05-26 | 东莞兰卫医学检验实验室有限公司 | EP manages rack combination |
| CN211142057U (en) * | 2019-11-11 | 2020-07-31 | 中国人民公安大学 | DNA sample case for criminal investigation |
| CN214881597U (en) * | 2021-01-21 | 2021-11-26 | 广州高盛智造科技有限公司 | Nucleic acid sample save set |
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