CN112831194B - Cochineal red lake without aluminum salt and preparation method thereof - Google Patents
Cochineal red lake without aluminum salt and preparation method thereof Download PDFInfo
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- CN112831194B CN112831194B CN202011573106.2A CN202011573106A CN112831194B CN 112831194 B CN112831194 B CN 112831194B CN 202011573106 A CN202011573106 A CN 202011573106A CN 112831194 B CN112831194 B CN 112831194B
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- cochineal
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- whey protein
- aluminum salt
- lake
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B63/00—Lakes
- C09B63/005—Metal lakes of dyes
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a cochineal red lake without aluminum salt and a preparation method thereof, wherein the preparation method comprises the following steps: preparing cochineal pigment solution; (2) preparing a whey protein solution; (3) Stirring and reacting cochineal red pigment solution and whey protein solution; (4) And cooling the reaction liquid to room temperature, filtering or centrifuging, collecting the solid, and drying to obtain the cochineal color lake without aluminum salt. Compared with the prior art, the method has the advantages of simple and feasible process, simple operation and lower cost, does not use aluminum salt or add calcium salt, directly utilizes calcium ions in protein to form chelation to obtain the cochineal red lake, and has good stability.
Description
Technical Field
The invention belongs to the field of natural pigment preparation, and particularly relates to cochineal red lake without aluminum salt and a preparation method thereof.
Background
Cochineal pigment is an anthraquinone natural pigment extracted from cochineal, and the color of carminic acid colorant can range from orange yellow to red to purple according to different pH values, so that the cochineal acid colorant is an excellent colorant for food, cosmetics, medicines and textiles, and is only one natural pigment which is allowed by FDA to be used for food, medicines and cosmetics. However, the maximum wavelength fluctuation range of the color of the carminic acid product is wide under different pH values, so that the application field of the carminic acid is limited. In order to solve this problem, CN101061185A, CN 101461513A, CN101475756a produces carminic acid aluminum calcium lake, which is a chelate lake compound formed by combining carminic acid with aluminum ions and calcium ions, and the lake is a product obtained by reacting carminic acid with aluminum ions and calcium ions under an aqueous condition.
However, the reason that aluminum ions cause memory loss of people is determined as food pollutants to be controlled, and the long-term accumulation of aluminum in the body of a patient with renal failure seriously threatens the bone, brain and nervous system of the patient. Therefore, there is an urgent need to develop a method for preparing an aluminum-free cochineal red lake.
Disclosure of Invention
Based on the current situation that cochineal color lake in the prior art mostly contains aluminum ions, the cochineal color lake without aluminum salt and the preparation method thereof are provided.
The purpose of the invention can be realized by the following technical scheme:
the invention provides a preparation method of cochineal red lake without aluminum salt, which comprises the following steps:
(1) Preparing a cochineal pigment solution;
(2) Preparing a whey protein solution;
(3) Stirring and reacting cochineal red pigment solution and whey protein solution;
(4) And cooling the reaction liquid to room temperature, filtering or centrifuging, collecting the solid, and drying to obtain the cochineal color lake without aluminum salt.
In one embodiment of the present invention, the method for preparing the cochineal color solution in step (1) comprises: the cochineal color with the purity of more than 90 percent is stirred and dissolved in deionized water, and the mass ratio of the cochineal color to the deionized water is 1:5-10.
In one embodiment of the present invention, the method for preparing the whey protein solution in step (2) comprises: dissolving whey protein in deionized water, wherein the mass ratio of the whey protein to the deionized water is 1:5-10.
In one embodiment of the present invention, the conditions for stirring and reacting the cochineal color solution and the whey protein solution in step (3) are as follows: heating the cochineal pigment solution to 85-95 ℃, slowly adding the whey protein solution, and stirring for reacting for 0.5-2h. Wherein the volume ratio of the cochineal color solution to the whey protein solution is 1 (1-5).
In one embodiment of the present invention, in the step (4), the temperature of the reaction solution is naturally reduced to room temperature.
In one embodiment of the present invention, in the step (4), the filtration comprises suction filtration or plate-and-frame filtration.
In one embodiment of the present invention, in the step (4), the centrifugation may be performed at 4000 revolutions.
In one embodiment of the present invention, in the step (4), the collected solid is dried in vacuum at 50 to 70 ℃.
In one embodiment of the present invention, in step (4), the collected solid is dried and then pulverized to 100-200 mesh, so as to obtain cochineal red lake without aluminum salt.
The invention also provides the cochineal red lake without aluminum salt, which is prepared by the preparation method.
The cochineal color lake without aluminum salt has no obvious color change between pH4.0-7.0, and is red.
Compared with the prior art, the method has the advantages of simple and feasible process, simple operation and lower cost, does not use aluminum salt or add calcium salt, directly utilizes calcium ions in protein to form chelation to obtain the cochineal red lake, and has good stability.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example 1
2.1g carminic acid was dissolved in 20ml water, and 3.3g whey protein was added to 30ml water and dissolved with heating and stirring. Then carmine solution was added and the temperature was raised to 85-95 c and whey protein solution was slowly added. Stirring and reacting for 1h, centrifuging for 10 minutes at 4000 revolutions, collecting solid, washing the solid for 1 time by using deionized water, vacuum-drying the collected solid at 60 ℃, and crushing to 100-200 meshes to obtain the cochineal lake with red color, wherein the color of the prepared cochineal lake is not obviously changed between pH4.0 and 7.0 and is red.
Example 2
This example provides a method for preparing cochineal red lake without aluminum salt, comprising the following steps:
(1) Preparation of cochineal pigment solution: stirring and dissolving cochineal pigment with the purity of more than 90% in deionized water, wherein the mass ratio of the cochineal pigment to the deionized water is 1:5;
(2) Preparing a whey protein solution: dissolving whey protein in deionized water, wherein the mass ratio of the whey protein to the deionized water is 1:5;
(3) Heating the cochineal pigment solution to 85 ℃, slowly adding the whey protein solution into the cochineal pigment solution, and stirring for reacting for 2 hours; wherein the volume ratio of the cochineal pigment solution to the whey protein solution is 1:3.
(4) Naturally cooling the reaction liquid to room temperature, carrying out suction filtration, collecting solids, carrying out vacuum drying at 50 ℃, and crushing to 100-200 meshes to obtain the cochineal color lake without aluminum salt.
The cochineal color lake obtained in this example, which does not contain aluminum salt, is red without any significant change in color between pH4.0 and 7.0.
Example 3
This example provides a method for preparing cochineal red lake without aluminum salt, comprising the following steps:
(1) Preparing a cochineal color solution: dissolving cochineal pigment with purity of more than 90% in deionized water under stirring, wherein the mass ratio of the cochineal pigment to the deionized water is 1:8;
(2) Preparing a whey protein solution: dissolving whey protein in deionized water, wherein the mass ratio of the whey protein to the deionized water is 1:8;
(3) Heating the cochineal color solution to 90 ℃, slowly adding the whey protein solution into the cochineal color solution, and stirring for reacting for 1 hour; wherein the volume ratio of the cochineal color solution to the whey protein solution is 1.
(4) Naturally cooling the reaction liquid to room temperature, filtering by a plate frame, collecting solids, drying in vacuum at 60 ℃, and crushing to 100-200 meshes to obtain the cochineal color lake without aluminum salt.
The cochineal color lake obtained in this example, which does not contain aluminum salt, is red without any significant change in color between pH4.0 and 7.0.
Example 4
This example provides a method for preparing cochineal red lake without aluminum salt, comprising the following steps:
(1) Preparation of cochineal pigment solution: stirring and dissolving cochineal pigment with the purity of more than 90% in deionized water, wherein the mass ratio of the cochineal pigment to the deionized water is 1:10;
(2) Preparing a whey protein solution: dissolving whey protein in deionized water, wherein the mass ratio of the whey protein to the deionized water is 1;
(3) Heating the cochineal color solution to 95 ℃, slowly adding the whey protein solution, and stirring for reacting for 0.5h; wherein the volume ratio of the cochineal pigment solution to the whey protein solution is 1:4.
(4) Naturally cooling the reaction liquid to room temperature, centrifuging at 4000 revolutions, collecting solids, performing vacuum drying at 70 ℃, and crushing to 100-200 meshes to obtain the cochineal color lake without aluminum salt.
The cochineal color lake obtained in this example, which does not contain aluminum salt, is red without any significant change in color between pH4.0 and 7.0.
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (3)
1. A preparation method of cochineal red lake without aluminum salt is characterized by comprising the following steps:
(1) Preparing a cochineal pigment solution;
(2) Preparing a whey protein solution;
(3) Stirring and reacting cochineal red pigment solution and whey protein solution;
(4) Cooling the reaction liquid to room temperature, filtering or centrifuging, collecting the solid, and drying to obtain the cochineal color lake without aluminum salt;
the method for preparing the cochineal pigment solution in the step (1) comprises the following steps: stirring and dissolving cochineal pigment with the purity of more than 90% in deionized water, wherein the mass ratio of the cochineal pigment to the deionized water is 1:5-10;
the method for preparing the whey protein solution in the step (2) comprises the following steps: dissolving whey protein in deionized water, wherein the mass ratio of the whey protein to the deionized water is 1:5-10;
the condition of stirring and reacting the cochineal red pigment solution and the whey protein solution in the step (3) is as follows: heating the cochineal color solution to 85-95 ℃, adding the whey protein solution, stirring and reacting for 0.5-2h, wherein the volume ratio of the cochineal color solution to the whey protein solution is 1 (1-5);
and (4) drying the collected solid in vacuum at 50-70 ℃, and in the step (4), drying the collected solid and then crushing the dried solid into 100-200 meshes to obtain the cochineal color lake without aluminum salt, wherein the color lake is red at the pH value of 4.0-7.0.
2. The method for preparing cochineal lake without aluminum salt according to claim 1, wherein in the step (4), the temperature of the reaction solution is naturally reduced to room temperature by cooling the reaction solution to room temperature.
3. The method according to claim 1, wherein the step (4) of filtering comprises suction filtration or plate-and-frame filtration.
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| CN202011573106.2A CN112831194B (en) | 2020-12-24 | 2020-12-24 | Cochineal red lake without aluminum salt and preparation method thereof |
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| CN202011573106.2A CN112831194B (en) | 2020-12-24 | 2020-12-24 | Cochineal red lake without aluminum salt and preparation method thereof |
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| CN112831194B true CN112831194B (en) | 2023-03-31 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR930012007B1 (en) * | 1990-12-17 | 1993-12-23 | 동양나일론 주식회사 | Process for the preparation of insoluble carminic acid lake |
| CN101461513B (en) * | 2009-01-05 | 2012-07-25 | 上海染料研究所有限公司 | Method for preparing water-soluble cochinilin color lake |
| CN111454588B (en) * | 2020-04-10 | 2021-12-07 | 江南大学 | Method for preparing cochineal red aluminum-free lake pigment by using protein, product and application thereof |
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