CN112747957A - Sample pretreatment device and treatment method for microbial detection - Google Patents
Sample pretreatment device and treatment method for microbial detection Download PDFInfo
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- CN112747957A CN112747957A CN202011612636.3A CN202011612636A CN112747957A CN 112747957 A CN112747957 A CN 112747957A CN 202011612636 A CN202011612636 A CN 202011612636A CN 112747957 A CN112747957 A CN 112747957A
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- 238000001514 detection method Methods 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 26
- 230000000813 microbial effect Effects 0.000 title claims description 16
- 244000005700 microbiome Species 0.000 claims abstract description 19
- 238000005070 sampling Methods 0.000 claims abstract description 12
- 239000002689 soil Substances 0.000 claims description 31
- 230000005540 biological transmission Effects 0.000 claims description 20
- 238000005553 drilling Methods 0.000 claims description 20
- 239000010410 layer Substances 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 11
- 238000007789 sealing Methods 0.000 claims description 10
- 230000000670 limiting effect Effects 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 6
- 239000002344 surface layer Substances 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 3
- 230000002906 microbiologic effect Effects 0.000 claims 1
- 125000003003 spiro group Chemical group 0.000 claims 1
- 230000003068 static effect Effects 0.000 abstract description 5
- 238000002203 pretreatment Methods 0.000 abstract description 4
- 230000002829 reductive effect Effects 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000013076 target substance Substances 0.000 description 2
- 241001465382 Physalis alkekengi Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
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- General Physics & Mathematics (AREA)
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- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to the technical field of microorganism sampling devices, in particular to a sample pretreatment device and a sample pretreatment method for microorganism detection, aiming at the problem that an object with a set depth cannot be directly sampled in the prior art, the invention provides the following scheme. The invention can ensure that the upper cover and the main pipe can be kept forbidden when the whole main pipe is spirally descended, can ensure that the main pipe and the upper cover are kept relatively static no matter the main pipe is drilled clockwise or lifted anticlockwise, and can not lead the upper cover and the main pipe to be more and more tightened in the initial screwing process to cause difficult disassembly in the later period.
Description
Technical Field
The invention relates to the technical field of microorganism sampling devices, in particular to a sample pretreatment device and a sample pretreatment method for microorganism detection.
Background
In detecting the presence of a microorganism or a target substance in a clinical sample, it is often necessary to process the sample to remove interfering substances and cells, or to enrich the target microorganism or other target substance for detection. Especially for high sensitivity detection, sample preparation is a crucial step, because the concentration of target microorganisms and interfering substances in the sample may cause a great influence on the detection result.
The sample collection device for the microorganism detection disclosed by the retrieved Chinese patent No. CN201822208700.6 comprises an operation table, wherein the bottom of the operation table is fixedly connected with supporting legs, the inside of the operation table is movably sleeved with a threaded rod, the outside of the threaded rod is meshed with the outer edge of a gear, the gear is fixedly sleeved on an output shaft of a transmission motor, the outside of the transmission motor is fixedly sleeved with a fixed sleeve, the transmission motor is electrically connected with a mains supply circuit, but the state is that a drill cylinder in a drilling device can only take out all samples from a preset depth to a surface layer together when the sampling is carried out, the device imitates a sampling mode of a Luoyang shovel, has a plurality of defects that when the object with the target depth is sampled, all the objects from the top end to the target layer can only be taken out, the original target layer can not be directly sampled, and waste is generated, and a quite long sampling tube needs to be designed, so that the sampling tube is easily mixed with a surface object during pulling, and the detection precision is influenced.
Disclosure of Invention
The invention provides a sample pretreatment device and a sample pretreatment method for microbial detection, aiming at overcoming the problem that an object with a given depth cannot be directly sampled in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme: a sample pretreatment device and a treatment method for microorganism detection comprise a female pipe, wherein the inner wall of the female pipe is provided with a smooth surface, an upper cover is screwed on the circumferential outer wall of the female pipe close to the top end, a screw hole is formed in the upper surface of the upper cover close to the pipe orifice of the female pipe, a fastening bolt is screwed in the screw hole, so that the upper cover and the female pipe can be kept forbidden when the female pipe is wholly screwed down, the female pipe and the upper cover can be kept relatively static no matter clockwise drilling down or anticlockwise lifting up, and the upper cover and the female pipe cannot be difficult to disassemble in the later period due to the increasing tightness of the screw connection between the upper cover and the female pipe, a mounting hole is formed in the middle part of the upper surface of the upper cover, a self-locking nut is welded in the mounting hole, a screw bolt is screwed in the self-locking nut, transmission rods which are coaxially distributed are fixed at the upper end and the, a wear-resistant positioning disc is fixed at the bottom end of the transmission rod close to the lower part, the outer wall of the wear-resistant positioning disc is rotatably connected with a soil separating cone, the lower half section of the soil-dividing cone is arranged into a conical structure, the circumferential inner wall of the main pipe close to the bottom end is in threaded connection with a first drill bit in a horn-shaped structure, the aperture of the first drill bit near the top end is smaller than that near the bottom end, the inner circumferential wall of the top end of the first drill bit is in sliding fit with the outer wall of the soil-dividing cone, a supporting top pipe is fixed at the top end of the upper cover, the inner diameter of the supporting top pipe is larger than the diameter of the transmission rod, thereby can only need press down from the top and support the push pipe and rotate on one side when needs sample the object when using and can the female pipe descends to the position of getting of boring, later with the pull-up of dividing the soil awl expose the mouth of pipe, continue to push down female pipe again and can carry out depthkeeping sampling to the effectual mixture that has prevented the different levels of material.
According to the preferable scheme of the invention, the annular groove is formed in the pipe orifice at the top end of the main pipe, the annular rubber ring is embedded in the annular groove, and the thickness of the rubber ring is equal to the depth of the annular groove, so that slipping is avoided when the bottom end of the fastening bolt is in contact with the rubber ring in use, and the locking effect on the upper cover is improved.
As a preferable scheme in the invention, the conical sleeve is fixed on the circumferential outer wall of the main pipe close to the bottom end, and the circumferential outer wall of the conical sleeve is provided with the first spiral groove, so that when an object to be sampled with high compactness is required to be sampled in use, the object can be slowly pushed to two sides in the process of drilling down, the contact between the upper half part of the main pipe and the object is reduced as much as possible, and the resistance generated in the process of pulling up is reduced.
According to the preferable scheme of the invention, three to five limiting grooves are formed in the circumferential outer wall of the soil separating cone close to the bottom end, and the limiting convex blocks matched with the limiting grooves are fixed on the inner wall of the first drill close to the top end, so that the soil separating cone cannot rush out of the pipe orifice when descending.
According to the preferable scheme, the inner wall of the circumference of the inclined plane of the first drill bit is provided with the second spiral grooves, the cross sections of the second spiral grooves are of a semicircular structure, the number of the second spiral grooves is three to six, and the distance between every two spiral grooves is the same everywhere, so that materials can be driven to slide upwards slowly in the process of rotating and drilling when the drill bit is used.
As a preferable scheme in the invention, four rectangular notches are formed in the circumferential inner wall of the first drill bit, which is close to the opening at the bottom end, and the width of each rectangular notch is as deep as a lamp groove, so that the first drill bit can be fixed and radially applied when being installed, and the tip ends of two support legs of one expansion clamp are separately supported on the two opposite rectangular notches, and then the expansion clamp is rotated to form a whole.
As a preferable scheme in the invention, the circumferential inner wall of the main pipe close to the bottom end is also in threaded connection with a second drill bit, the diameter of the second drill bit close to the bottom end is smaller than that of the second drill bit close to the top end, and the circumferential outer wall of the second drill bit close to the bottom end is provided with a hexagonal cutting shoulder, so that the second drill bit can be directly sleeved on the outer wall of the second drill bit by a vice or a small wrench when being installed and then rotated, the outer wall of the second drill bit close to the bottom end edge is provided with a rounded corner, and the top end of the bottom of the soil-dividing cone is completely exposed outside, so that the resistance when the soil-dividing cone is wholly lowered can be reduced when the soil.
As a preferable scheme in the invention, the anti-slip groove is arranged on the circumferential outer wall of the soil-dividing cone, and the sealing ring is embedded in the anti-slip groove, so that when the soil-dividing cone is used, if a fluid or an object with low compactness is sampled, the sealing ring needs to be sleeved on the outer wall of the soil-dividing cone, and the sample is prevented from flowing away from a gap between the soil-dividing cone and the inner wall of the drill bit when the sample is pulled upwards.
A method for pretreating a sample for detecting microorganisms, comprising the steps of:
the method comprises the following steps: firstly, placing a sampling device above an object to be sampled, selecting a flat place for drilling, and then predicting the density of the sample, whether an internal sample layer is fluid or not, whether the compactness needs to be changed or not and which way to drill for analysis;
step two: for the object with higher surface compactness, a first drilling mode, namely a second drill with a small head facing downwards and a large head, is adopted; for an object with lower surface compactness, a second drilling mode, namely a first drill bit with a downward large opening, is adopted, and if a sample layer is fluid, a sealing ring needs to be sleeved on the outer wall of the soil separation cone;
step three: the soil separating cone is lowered to the pipe orifice at the bottom end of the drill bit from the top end of the supporting top pipe through the rotary transmission rod, the pointed end is exposed completely, then the upper cover of the main pipe is locked, and the supporting top pipe can be pressed downwards in a rotary mode until the lowering depth of the bottom end of the main pipe reaches the upper surface layer of the preset position;
step four: and then the transmission rod is rotated reversely to lift the soil separating cone to the top end of the main pipe, so that the soil separating cone can be continuously pressed down to sample, and the soil separating cone is lowered to the pipe orifice of the drill bit at the bottom end to plug the pipe orifice and then the main pipe is pulled back under the condition that the sample layer is fluid.
To sum up, the beneficial effect in this scheme is:
1. according to the sample pretreatment device and the sample pretreatment method for microbial detection, the screw holes formed in the circumferential edge of the upper cover and the fastening bolts screwed in the screw holes are arranged, the rubber rings embedded in the pipe orifices at the top end of the mother pipe are matched with the lower parts of the fastening bolts, when the mother pipe and the upper cover are required to be kept relatively static, the upper cover can be fixed regardless of whether the upper cover is screwed and fastened, namely, the upper cover and the mother pipe can be kept relatively forbidden when the whole mother pipe is spirally descended, and the mother pipe and the upper cover can be kept relatively static regardless of clockwise descending or counterclockwise ascending, and the situation that the upper cover and the mother pipe are increasingly tightened in the initial screwing process to cause later-stage difficult disassembly is avoided;
2. according to the sample pretreatment device and the treatment method for microorganism detection, the first drill bit and the second drill bit which are screwed in different directions at the pipe orifice at the bottom end of the main pipe and the soil separating cone which is inserted at the pipe orifice at the bottom end of the first drill bit and the second drill bit are arranged, the soil separating cone effectively blocks the pipe orifice at the bottom end of the drill bit when integrally descends, and provides a function of tapping, so that when an object needs to be sampled in use, the main pipe can be driven to descend to a drilling position only by pressing the supporting top pipe downwards from the top end and rotating the supporting top pipe, then the soil separating cone is pulled up to the pipe orifice far away from the drill bit, and then the main pipe is continuously pressed downwards to perform depth-setting sampling, so that different layers of materials are effectively prevented from being mixed;
3. according to the sample pretreatment device and the treatment method for microbial detection, through the arrangement of different drill bits and the conical sleeve sleeved on the outer wall of the main pipe close to the bottom end, and the cooperation of the spiral groove I formed in the outer wall of the conical sleeve, when an object to be sampled with high compactness is required to be sampled in use, the object can be slowly pushed to two sides in the drilling process, so that the contact between the upper half part of the main pipe and the object is reduced as much as possible, and the resistance generated in the pulling-up process is reduced;
4. according to the sample pretreatment device and the treatment method for microbial detection, the soil-dividing cone with the anti-skid groove and the sealing ring sleeved on the outer wall of the soil-dividing cone are arranged on the outer wall, so that when the device is used, if a fluid or an object with low compactness is sampled, the sealing ring is sleeved on the outer wall of the soil-dividing cone, and the sample is prevented from flowing away from a gap between the soil-dividing cone and the inner wall of a drill bit when the sample is pulled upwards.
Drawings
FIG. 1 is a schematic flow chart of a method for pretreating a sample for detecting microorganisms according to the present invention;
FIG. 2 is a schematic cross-sectional view of a first embodiment of a sample pretreatment device for microbial detection according to the present invention;
FIG. 3 is a schematic perspective sectional view of a first drill of a sample pretreatment device for microbial detection according to the present invention;
FIG. 4 is a schematic structural view of a second embodiment of a sample pretreatment device for microbial detection according to the present invention;
FIG. 5 is a schematic perspective view of a second drill of a sample pretreatment device for microbial detection according to the present invention;
FIG. 6 is a schematic view of a partial cross-sectional three-dimensional structure of a soil-dividing cone of a sample pretreatment device for microbial detection according to the present invention;
FIG. 7 is an enlarged schematic view of a sample pretreatment device for microbial detection shown in FIG. 2.
In the figure: the device comprises a main pipe 1, a conical sleeve 2, a first spiral groove 3, a first drill bit 4, a second spiral groove 5, a rectangular notch 6, a limiting groove 7, a soil-dividing cone 8, an inverted T-shaped rotary groove 801, a limiting bump 9, a wear-resistant positioning disc 10, a transmission rod 11, a stud 12, an upper cover 13, a self-locking nut 14, a support top pipe 15, a fastening bolt 16, a rubber ring 17, a second drill bit 18, a hexagonal shoulder cutter 1801 and a round corner 1802.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1
Referring to fig. 1-7, a sample pretreatment device and a treatment method for microorganism detection, comprising a main pipe 1 with a smooth inner wall, an upper cover 13 screwed on the outer wall of the circumference of the main pipe 1 near the top end, a screw hole provided on the upper surface of the upper cover 13 near the pipe orifice of the main pipe 1, and a fastening bolt 16 screwed in the screw hole, so as to ensure that the upper cover 13 and the main pipe 1 can keep relative prohibition when the main pipe 1 is spirally descended, and ensure that the main pipe 1 and the upper cover 13 keep relative static regardless of clockwise drilling down or counterclockwise lifting up, and will not make the screw connection between the upper cover 13 and the main pipe 1 more and more tight, which results in difficult later period disassembly, a mounting hole is provided in the middle of the upper surface of the upper cover 13, and a self-locking nut 14 is welded in the mounting hole, and a stud 12 is screwed in the self-locking nut 14, and transmission rods 11 coaxially distributed are respectively fixed on the upper and lower ends of the stud 12, the diameter of the transmission rod 11 is smaller than that of the stud 12, a wear-resistant positioning disc 10 is fixed at the bottom end of the transmission rod 11 close to the lower part, a soil separating cone 8 is rotatably connected to the outer wall of the wear-resistant positioning disc 10, the lower half section of the soil separating cone 8 is set to be in a conical structure, a first drill bit 4 in a horn-shaped structure is screwed on the circumferential inner wall of the mother pipe 1 close to the bottom end, the aperture of the first drill bit 4 close to the top end is smaller than that of the first drill bit 4 close to the bottom end, the circumferential inner wall of the top end of the first drill bit 4 is in sliding fit with the outer wall of the soil separating cone 8, a support top pipe 15 is fixed at the top end of the upper cover 13, the inner diameter of the support top pipe 15 is larger than that of the transmission rod 11, so that when an object needs to be sampled, the mother pipe 1 can be lowered to a drilling position by pressing the support top pipe 15 downwards from one, and then the main pipe 1 is pressed down continuously to perform depth-fixed sampling, thereby effectively preventing the mixing of materials at different levels.
In the invention, the top end pipe orifice of the main pipe 1 is provided with an annular groove, an annular rubber ring 17 is embedded in the annular groove, and the thickness of the rubber ring 17 is equal to the groove depth of the annular groove, so that the slipping is avoided when the bottom end of the fastening bolt 16 is contacted with the rubber ring 17 in use, and the locking effect on the upper cover 13 is improved.
Wherein, female pipe 1's circumference outer wall be close to the bottom mounting and have toper cover 2, and the circumference outer wall of toper cover 2 is opened and is had a helicla flute 3 to can be when using when the higher object of treating the sample of degree of compactness is taken a sample, can slowly promote the object to both sides at the in-process of boring down, in order to let female pipe 1's first half minimize with the contact of object, the resistance that produces when reducing and pulling up.
Wherein, the circumference outer wall of branch soil awl 8 be close to the bottom and open and have three to five spacing grooves 7, and the inner wall of first drill bit 4 is close to the top and is fixed with the spacing lug 9 with spacing groove 7 mutual adaptation to can let branch soil awl 8 can not rush out the mouth of pipe when descending.
The inner wall of the circumference of the inclined plane of the first drill bit 4 is provided with two spiral grooves 5, the cross sections of the two spiral grooves 5 are of semicircular structures, the number of the two spiral grooves 5 is three to six, and the distance between every two spiral grooves 5 is the same everywhere, so that materials can be driven to slide upwards slowly in the process of rotating and drilling when the drill bit is used.
The circumference inner wall of first drill bit 4 open near the bottom opening part and have four equidistance rectangular gaps 6 that distribute, and the width lamps and lanterns groove depth of rectangular gap 6 is favorable to fixing it when installing first drill bit 4 and radially exerting force, only need the most advanced of two stabilizer blades that press from both sides with an inflation separately the top at two relative rectangular gaps 6, on, later the rotatory inflation presss from both sides wholly can.
A method for pretreating a sample for detecting microorganisms, comprising the steps of:
the method comprises the following steps: firstly, placing a sampling device above an object to be sampled, selecting a flat place for drilling, and then predicting the density of the sample, whether an internal sample layer is fluid or not, whether the compactness needs to be changed or not and which way to drill for analysis;
step two: for the object with higher surface compactness, a first drilling mode, namely a second drill with a small head facing downwards and a large head, is adopted; for an object with lower surface compactness, a second drilling mode, namely a first drill bit with a downward large opening, is adopted, and if a sample layer is fluid, a sealing ring needs to be sleeved on the outer wall of the soil separation cone;
step three: the soil separating cone is lowered to the pipe orifice at the bottom end of the drill bit from the top end of the supporting top pipe through the rotary transmission rod, the pointed end is exposed completely, then the upper cover of the main pipe is locked, and the supporting top pipe can be pressed downwards in a rotary mode until the lowering depth of the bottom end of the main pipe reaches the upper surface layer of the preset position;
step four: and then the transmission rod is rotated reversely to lift the soil separating cone to the top end of the main pipe, so that the soil separating cone can be continuously pressed down to sample, and the soil separating cone is lowered to the pipe orifice of the drill bit at the bottom end to plug the pipe orifice and then the main pipe is pulled back under the condition that the sample layer is fluid.
Example 2
Referring to fig. 1-3, a sample pretreatment device and a treatment method for microbial detection, in this embodiment, compared to embodiment 1, the device further includes a second drill 18 screwed to the inner circumferential wall of the female pipe 1 near the bottom end, the diameter of the second drill 18 near the bottom end is smaller than the diameter near the top end, and a hexagonal shoulder 1801 is formed on the outer circumferential wall of the second drill 18 near the bottom end, which is beneficial to directly sleeve the second drill 18 on the outer wall of the second drill 18 by a vice or a small wrench when the second drill 18 is installed, and then the second drill 18 is rotated, wherein the outer wall of the second drill 18 near the bottom end is provided with a rounded corner 1802, and the top end of the bottom of the soil-separating cone 8 is completely exposed, so that the resistance when the whole body descends can be reduced, the pressure is increased, and the drilling efficiency is improved.
Wherein, the circumference outer wall of branch soil awl 8 be provided with anti-skidding groove, and the embedded sealing washer that is equipped with in anti-skidding groove to can be when using, if when taking a sample to the less object of fluidics or degree of compactness, need connect the sealing washer in the outer wall cover of branch soil awl 8, the sample flows away from the gap department between branch soil awl 8 and the drill bit inner wall when avoiding upwards pulling out.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (9)
1. A sample pretreatment device for microbial detection comprises a female pipe (1) with an inner wall provided with a smooth surface, wherein an upper cover (13) is screwed on the outer wall of the circumference of the female pipe (1) close to the top end, the upper surface of the upper cover (13) is provided with a screw hole close to the pipe orifice of the female pipe (1), a fastening bolt (16) is screwed in the screw hole, the middle part of the upper surface of the upper cover (13) is provided with a mounting hole, a self-locking nut (14) is welded in the mounting hole, a stud (12) is screwed in the self-locking nut (14), the upper end and the lower end of the stud (12) are respectively fixed with a coaxially distributed transmission rod (11), the diameter of the transmission rod (11) is smaller than that of the stud (12), a wear-resistant positioning disc (10) is fixed at the bottom end of the transmission rod (11) close to the lower part, and is characterized in that the outer wall of the wear-resistant positioning disc, and divide the lower half section of native awl (8) to set to conical structure, the circumference inner wall of female pipe (1) is close to the bottom spiro union have be loudspeaker column structure first drill bit (4), and first drill bit (4) are close to the aperture on top and are less than the aperture that is close to the bottom, and first drill bit (4) top circumference inner wall forms sliding fit with the outer wall of branch native awl (8), and the top of upper cover (13) is fixed with supports push pipe (15), and the internal diameter that supports push pipe (15) is greater than the diameter of transfer line (11).
2. The pretreatment device for the sample for the microbial detection according to claim 1, wherein an annular groove is formed at the top end pipe orifice of the mother pipe (1), an annular rubber ring (17) is embedded in the annular groove, and the thickness of the rubber ring (17) is equal to the groove depth of the annular groove.
3. The pretreatment device for the sample for the microbial detection according to claim 1, wherein a conical sleeve (2) is fixed on the circumferential outer wall of the mother pipe (1) near the bottom end, and a first spiral groove (3) is formed in the circumferential outer wall of the conical sleeve (2).
4. The pretreatment device for a sample for microorganism detection according to claim 1, wherein the outer circumferential wall of the soil-dividing cone (8) has three to five limiting grooves (7) near the bottom end, and the inner wall of the first drill (4) has a limiting projection (9) fitted with the limiting grooves (7) near the top end.
5. The pretreatment device of a sample for microorganism detection according to claim 1, wherein the inner wall of the circumference of the inclined surface of the first drill (4) is provided with a second spiral groove (5), the cross section of the second spiral groove (5) is of a semicircular structure, the number of the second spiral grooves (5) is three to six, and the distance between each second spiral groove (5) is the same everywhere, so that the materials can be driven to slide upwards slowly in the process of rotating and drilling.
6. The pretreatment device for the samples for the microorganism detection according to claim 1, wherein four rectangular notches (6) are formed in the inner circumferential wall of the first drill (4) near the bottom opening and are distributed equidistantly, and the width of each rectangular notch (6) is as deep as a lamp, so that the first drill (4) can be fixed and radially applied when being installed, the tips of two support legs of one expansion clamp are only required to be separately supported on the two opposite rectangular notches (6), and then the expansion clamp is rotated integrally.
7. The sample pretreatment device for microorganism detection according to claim 1, wherein a second drill bit (18) is further screwed on the inner circumferential wall of the female pipe (1) near the bottom end, the diameter of the second drill bit (18) near the bottom end is smaller than that near the top end, a hexagonal shoulder (1801) is formed on the outer circumferential wall of the second drill bit (18) near the bottom end, so that when the second drill bit (18) is installed, a vice or a small wrench is directly sleeved on the outer wall of the second drill bit (18), and then the second drill bit is rotated, rounded corners (1802) are formed on the outer wall of the second drill bit (18) near the bottom end edge, and the top end of the bottom of the soil-separating cone (8) is completely exposed.
8. The pretreatment device for the samples for the microbiological detection according to claim 1, wherein the outer circumferential wall of the soil-dividing cone (8) is provided with an anti-slip groove, and a sealing ring is embedded in the anti-slip groove.
9. A method for pretreating a sample for detecting microorganisms, comprising the steps of:
the method comprises the following steps: firstly, placing a sampling device above an object to be sampled, selecting a flat place for drilling, and then predicting the density of the sample, whether an internal sample layer is fluid or not, whether the compactness needs to be changed or not and which way to drill for analysis;
step two: for the object with higher surface compactness, a first drilling mode, namely a second drill with a small head facing downwards and a large head, is adopted; for an object with lower surface compactness, a second drilling mode, namely a first drill bit with a downward large opening, is adopted, and if a sample layer is fluid, a sealing ring needs to be sleeved on the outer wall of the soil separation cone;
step three: the soil separating cone is lowered to the pipe orifice at the bottom end of the drill bit from the top end of the supporting top pipe through the rotary transmission rod, the pointed end is exposed completely, then the upper cover of the main pipe is locked, and the supporting top pipe can be pressed downwards in a rotary mode until the lowering depth of the bottom end of the main pipe reaches the upper surface layer of the preset position;
step four: and then the transmission rod is rotated reversely to lift the soil separating cone to the top end of the main pipe, so that the soil separating cone can be continuously pressed down to sample, and the soil separating cone is lowered to the pipe orifice of the drill bit at the bottom end to plug the pipe orifice and then the main pipe is pulled back under the condition that the sample layer is fluid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011612636.3A CN112747957A (en) | 2020-12-30 | 2020-12-30 | Sample pretreatment device and treatment method for microbial detection |
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| CN202011612636.3A CN112747957A (en) | 2020-12-30 | 2020-12-30 | Sample pretreatment device and treatment method for microbial detection |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114216719A (en) * | 2021-12-09 | 2022-03-22 | 重庆地质矿产研究院 | Rotary sampler for geological exploration |
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2020
- 2020-12-30 CN CN202011612636.3A patent/CN112747957A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114216719A (en) * | 2021-12-09 | 2022-03-22 | 重庆地质矿产研究院 | Rotary sampler for geological exploration |
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Application publication date: 20210504 |