CN112741918A - Cell culture dish sterilization and disinfection method - Google Patents
Cell culture dish sterilization and disinfection method Download PDFInfo
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- CN112741918A CN112741918A CN202110110504.9A CN202110110504A CN112741918A CN 112741918 A CN112741918 A CN 112741918A CN 202110110504 A CN202110110504 A CN 202110110504A CN 112741918 A CN112741918 A CN 112741918A
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- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 80
- 238000004113 cell culture Methods 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000007789 sealing Methods 0.000 claims abstract description 68
- 230000001954 sterilising effect Effects 0.000 claims abstract description 33
- 238000004140 cleaning Methods 0.000 claims abstract description 22
- 230000000249 desinfective effect Effects 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims description 26
- 238000009434 installation Methods 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000008602 contraction Effects 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 5
- 125000003003 spiro group Chemical group 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 230000003028 elevating effect Effects 0.000 claims description 2
- 230000008676 import Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 8
- 241000894006 Bacteria Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 239000011521 glass Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 238000005096 rolling process Methods 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 1
- 238000004115 adherent culture Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000007514 turning Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
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- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cell culture dish sterilization and disinfection method, which adopts the following disinfection equipment, wherein the cell culture dish sterilization and disinfection equipment comprises a sealing box, an inlet and an outlet, a sealing door, a disinfection lamp, a rotating mechanism, a lifting mechanism, a placing mechanism and a cleaning machine. The present invention can solve the following problems: when the existing cell culture dish sterilization and disinfection equipment is used for sterilizing and disinfecting a cell culture dish, a large number of dead corners exist due to uneven irradiation of ultraviolet rays on the culture dish, so that the culture dish is not thoroughly sterilized and disinfected; secondly, the existing cell culture dish sterilization and disinfection equipment is unstable in placing and fixing culture dishes and complex in operation, and can not be placed in batches. The invention can enable the culture dish to receive uniform ultraviolet irradiation, increases the sterilization and disinfection effects, can stably place the culture dish in batches, and has simple operation and convenient use.
Description
Technical Field
The invention relates to the technical field of sterilization and disinfection, in particular to a sterilization and disinfection method for a cell culture dish.
Background
A petri dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic. The culture dish is basically made of two types, mainly plastic and glass, and the glass can be used for plant materials, microorganism culture and adherent culture of animal cells. The plastic may be of polyethylene material, disposable and reusable, suitable for laboratory inoculation, streaking, bacterial isolation and may be used for the cultivation of plant material. Before the cell culture dish is used, sterilization and disinfection are needed, harmful substances are directly contacted with cells, the cells are very sensitive to any harmful substances, and few residues can generate toxic and side effects on the cells.
However, the existing cell culture dish sterilization and disinfection equipment has the following problems when the cell culture dish is sterilized and disinfected: when the existing cell culture dish sterilization and disinfection equipment is used for sterilizing and disinfecting a cell culture dish, a large number of dead corners exist due to uneven irradiation of ultraviolet rays on the culture dish, so that the culture dish is not thoroughly sterilized and disinfected; secondly, the existing cell culture dish sterilization and disinfection equipment is unstable in placing and fixing culture dishes and complex in operation, and can not be placed in batches.
Disclosure of Invention
In order to solve the problems, the invention provides a method for sterilizing and disinfecting a cell culture dish, which can solve the problems mentioned above.
In order to achieve the purpose, the invention adopts the following technical scheme to realize the purpose: a cell culture dish sterilization and disinfection method adopts the following disinfection equipment, the cell culture dish sterilization and disinfection equipment comprises a seal box, an inlet and an outlet, a seal door, a disinfection lamp, a rotating mechanism, a lifting mechanism, a placing mechanism and a cleaning mechanism, the seal box is of a box body structure, the upper side of the right end face of the seal box is provided with the inlet and the outlet which are communicated with each other inside and outside, the outer wall of the seal box positioned at the inlet and the outlet is hinged with the seal door, the inner side wall of the upper side of the seal box is provided with the disinfection lamp, the rotating mechanism and the lifting mechanism are arranged at the upper side of the seal box, the placing mechanism is arranged on the lifting mechanism, and the lower side of; wherein:
the rotating mechanism comprises a rotating fluted disc, a first driving motor, a driving gear, a fixed shaft, a fixed gear, a rotating rod and a rotating wheel, the rotating fluted disc is connected to the top in the sealed box in a rotating fit manner, the rotating fluted disc is internally of a hollow structure, the upper end surface of the seal box is provided with a first driving motor, one end, entering the seal box, of an output shaft of the first driving motor is provided with a driving gear, the driving gear and the rotating fluted disc are mutually meshed, the top in the seal box is provided with a fixed shaft, the axis of the fixed shaft and the axis of the rotating fluted disc are on the same line, one end, entering the rotating fluted disc, of the fixed shaft is provided with a fixed gear, the lower end surface of the rotating fluted disc is uniformly arranged along the circumferential direction of the rotating fluted disc and is provided with a rotating rod in a rotating connection mode, one end, entering the rotating fluted disc, of the upper end of the rotating;
the lifting mechanism comprises a fixed block, a screw rod, a second driving motor, a lifting plate, a rotating disc and driven rods, wherein the fixed block is symmetrically arranged on the front side wall and the rear side wall of the sealing box, which are close to the middle position, the screw rod is arranged between the fixed block and the upper side wall of the sealing box in a rotating fit manner, four corners of the lifting plate are correspondingly screwed on the screw rod, the rotating disc is arranged in the middle of the lifting plate in a rotating fit manner, the driven rods are uniformly arranged on the rotating disc along the circumferential direction of the rotating disc and are in rotating connection, the number and the positions of the driven rods correspond to the rotating rods, and the driven rods are in sliding connection with the;
the placing mechanism comprises a first mounting plate, a second mounting plate, an arc chute, a first limiting rod, a shifting gear, a connecting rod, a first transmission gear, a contraction spring, a second limiting rod, a fixed rod and a placing ring, wherein the first mounting plate is arranged at the lower end of the driven rod, the second mounting plate is arranged on the driven rod on the upper side of the first mounting plate, the second mounting plate is of a hollow structure, the arc chute is symmetrically arranged on one side of the first mounting plate, which is opposite to the second mounting plate, and is far away from the driven rod, the first limiting rod is arranged between the two arc chutes of the first mounting plate, which are opposite to the second mounting plate in a sliding manner, the shifting gear is symmetrically arranged in the second mounting plate in a rotating fit manner, the central axis of the shifting gear and the arc center of the arc chute are on the same straight line, the upper end of the first limiting rod extends into the second, one end of the upper end of the first limiting rod, which extends into the second mounting plate, is connected through a contraction spring, first transmission gears are symmetrically arranged in the second mounting plate between the shifting teeth in a rotating fit mode, the first transmission gears are mutually meshed, the shifting gears are mutually meshed with one first transmission gear close to the shifting gears, the second limiting rod is symmetrically arranged between opposite surfaces of the first mounting plate and the second mounting plate and close to the driven rod, fixed rods are symmetrically arranged between opposite surfaces of the first mounting plate and the second mounting plate between the first limiting rod and the second limiting rod, and a placing ring is linearly and uniformly arranged between the two fixed rods from top to bottom;
when the cell culture dish sterilization and disinfection equipment is used for sterilizing and disinfecting the cell culture dish, the method comprises the following steps:
s1, placing a culture dish: placing and fixing the culture dish to be sterilized and disinfected through a placing mechanism, and closing a sealing door;
s2, washing a culture dish: the placing mechanism is driven to descend into the disinfection solution through the lifting mechanism, the culture dish is soaked in the disinfection solution for disinfection, and meanwhile, the driven rod is driven to revolve and rotate around the axial lead of the rotary fluted disc through the rotating mechanism, so that the placing mechanism is driven to stir in the disinfection solution, and meanwhile, the placing mechanism is matched with the cleaning mechanism to clean the inner wall of the culture dish;
s3, ultraviolet sterilization: the placing mechanism is driven to ascend to the upper side of the sealing box through the lifting mechanism, the culture dish is irradiated, sterilized and disinfected by ultraviolet light emitted by the disinfection lamp, meanwhile, the placing mechanism is driven to rotate by the rotating mechanism, so that the culture dish is uniformly irradiated with ultraviolet rays, the culture dish throws out moisture during rotation, and ultraviolet irradiation is finished after a certain time;
s4, vacuum sterilization: vacuumizing the air in the sealed box for sterilization while S3 is performed;
s5, taking out the culture dish: and opening the sealing door after the disinfection is finished, and taking out the culture dish from the placing mechanism.
As a preferred technical scheme of the invention, the cleaning mechanism comprises an installation box, an electric push rod, a sliding plate, a telescopic shaft, a cleaning brush, a second transmission gear and a third driving motor, wherein the installation box is of a box-shaped structure with an opening at the right end, the installation box is installed on the lower side of the left end face of the sealing box, the electric push rod is installed on the left side wall of the installation box, the sliding plate is arranged in the installation box in a sliding manner, a telescopic rod of the electric push rod extends into the installation box and is connected to the sliding plate, the telescopic shafts are arranged on the sliding plate in a rotating fit manner and are uniformly and linearly arranged, the distance between the telescopic shafts is the same as the distance between the placing rings, one end of the telescopic shaft, which is far away from the sliding plate, penetrates through the side wall of the sealing box to enter the sealing box and is provided with, and the third driving motor is arranged on the left side of the mounting plate, and an output shaft of the third driving motor is connected with a telescopic shaft on the uppermost side of the sliding plate.
As a preferred technical scheme of the invention, the left side wall of the seal box is provided with a vacuum pump, an air suction port of the vacuum pump is communicated with the interior of the seal box, and an air inlet valve is arranged on the side wall of the seal box at one side of the vacuum pump.
As a preferred technical scheme, one end of the sealing door, which is far away from the hinged part with the sealing box, is provided with a clamping block, the clamping block is provided with a clamping groove, the side wall of the sealing box, which is on the same side as the clamping block, is provided with a hinged block, one end of a threaded rod is hinged to the hinged block, the other end of the threaded rod is in threaded connection with a hand wheel, and the threaded rod is matched with the clamping groove.
As a preferable technical scheme of the invention, a sealing gasket is embedded on the sealing box around the outer side of the inlet and the outlet.
In a preferred embodiment of the present invention, a water supply valve and a water discharge valve are disposed on a lower side of the right side wall of the seal box. The invention has the beneficial effects that:
1. the invention can solve the following problems of the existing cell culture dish sterilization and disinfection equipment when sterilizing and disinfecting the cell culture dish: when the existing cell culture dish sterilization and disinfection equipment is used for sterilizing and disinfecting a cell culture dish, a large number of dead corners exist due to uneven irradiation of ultraviolet rays on the culture dish, so that the culture dish is not thoroughly sterilized and disinfected; secondly, the existing cell culture dish sterilization and disinfection equipment is unstable in placing and fixing culture dishes and complex in operation, and can not be placed in batches. The invention can enable the culture dish to receive uniform ultraviolet irradiation, increases the sterilization and disinfection effects, can stably place the culture dish in batches, and has simple operation and convenient use.
2. The vacuum pump works, the vacuum pump vacuumizes the inside of the sealing box, aerobic bacteria on the culture dish are killed, and the disinfection effect on the culture dish is improved by reducing the survival conditions of the bacteria.
3. The placing mechanism is driven by the lifting mechanism to descend into the disinfection solution, the culture dish is soaked in the disinfection solution for disinfection, and meanwhile, the driven rod is driven by the rotating mechanism to revolve and rotate around the axial lead of the rotating fluted disc, so that the placing mechanism is driven to stir in the disinfection solution, the culture dish can be in contact with the disinfection solution fully to wash and disinfect, and stains on the culture dish can be washed away.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a flow chart of the present invention;
FIG. 2 is a schematic cross-sectional view of the present invention;
FIG. 3 is an enlarged view of a portion of FIG. 2 in accordance with the present invention;
FIG. 4 is a schematic view of the placement mechanism of the present invention;
FIG. 5 is a cross-sectional view taken along line B-B of FIG. 2 in accordance with the present invention;
FIG. 6 is a schematic view of the overall structure of the present invention;
fig. 7 is a partial enlarged view of the invention at C of fig. 6.
Detailed Description
The embodiments of the invention will be described in detail below with reference to the drawings, but the invention can be implemented in many different ways as defined and covered by the claims.
As shown in fig. 1 to 7, a cell culture dish sterilization method adopts a sterilization device, the cell culture dish sterilization device comprises a seal box 1, an inlet and an outlet 2, a seal door 3, a sterilization lamp 4, a rotating mechanism 5, a lifting mechanism 6, a placing mechanism 7 and a cleaning mechanism 8, the seal box 1 is of a box structure, the inlet and the outlet 2 which are communicated with each other inside and outside are arranged on the upper side of the right end face of the seal box 1, the seal door 3 is hinged on the outer wall of the seal box 1 at the position of the inlet and the outlet 2, the sterilization lamp 4 is arranged on the inner side wall of the upper side of the seal box 1, the rotating mechanism 5 and the lifting mechanism 6 are arranged on the upper side of the seal box 1, the placing mechanism 7 is arranged on the lifting mechanism 6, and the cleaning mechanism 8 is arranged on the lower side; wherein:
the placing mechanism 7 comprises a first mounting plate 71, a second mounting plate 72, an arc-shaped sliding groove 73, a first limiting rod 74, a toggle gear 75, a connecting rod 76, a first transmission gear 77, a contraction spring 78, a second limiting rod 79, a fixed rod 710 and a placing ring 711, wherein the first mounting plate 71 is arranged at the lower end of the driven rod 66, the second mounting plate 72 is arranged on the driven rod 66 on the upper side of the first mounting plate 71, the second mounting plate 72 is of a hollow structure, the arc-shaped sliding grooves 73 are symmetrically formed in the opposite surfaces of the first mounting plate 71 and the second mounting plate 72 and are far away from one side of the driven rod 66, the first limiting rod 74 is arranged between the two arc-shaped sliding grooves 73 opposite to the first mounting plate 71 and the second mounting plate 72 in a sliding mode, the toggle gear 75 is symmetrically arranged in the second mounting plate 72 in a rotating fit mode, and the center shaft of the toggle gear 75 and the, the upper end of a first limiting rod 74 extends into a second mounting plate 72 and is connected to the side wall of the toggle gear 75 through a connecting rod 76, one end of the first limiting rod 74 extending into the second mounting plate 72 is connected through a contraction spring 78, first transmission gears 77 are symmetrically arranged in the second mounting plate 72 between the toggle teeth in a rotating fit mode, the first transmission gears 77 are meshed with each other, the toggle gear 75 is meshed with one first transmission gear 77 close to the first transmission gears 77, second limiting rods 79 are symmetrically arranged between opposite surfaces of the first mounting plate 71 and the second mounting plate 72 and close to the driven rod 66, fixing rods 710 are symmetrically arranged between the opposite surfaces of the first mounting plate 71 and the second mounting plate 72 between the first limiting rod 74 and the second limiting rods 79, and placing rings 711 are uniformly arranged between the two fixing rods 710 from top to bottom. When the culture dish disinfection device works, the sealing door 3 is opened, the first limiting rod 74 is shifted, the first limiting rod slides relatively to the outer side in the arc sliding groove 73, the first limiting rod 74 drives the shifting gear 75 to rotate through the connecting rod, the shifting gear 75 drives the other shifting gear 75 to rotate through the transmission between the shifting gear 75 and the second transmission gear 86, so that the other shifting gear 75 enables the two first limiting rods 74 to be far away from each other in the arc sliding groove 73 through the connecting rod, after the first limiting rod 74 is shifted, culture dishes needing disinfection are sequentially placed into the placing ring 711, the second limiting rod 79 abuts against the bottom of the placed culture dishes, the first limiting rod 74 is loosened, the contraction spring 78 contracts and pulls the two first limiting rods 74 to slide close to each other in the arc sliding groove 73, so that the first limiting rods 74 abut against the openings of the culture dishes, batch stable placement of the culture dishes is achieved, and the operation is simple and convenient, moreover, the first limiting rod 74, the second limiting rod 79 and the placing ring 711 have small shielding area for the culture dish, and can reduce the irradiation dead angle when receiving ultraviolet light.
Sealing door 3 is kept away from and is provided with joint piece 31 with the articulated one end of 1 articulated department of seal box, has seted up joint groove 32 on the joint piece 31, is provided with articulated piece 13 on the 1 lateral wall of seal box with joint piece 31 with one side, and 14 one ends of threaded rod articulate at articulated piece 13, and the other end spiro union of threaded rod 14 has hand wheel 13, and threaded rod 14 and joint groove 32 cooperation. During specific work, when the sealing door 3 is closed, the threaded rod 14 is correspondingly clamped into the clamping groove 32, the hand wheel 13 is manually rotated, the hand wheel 13 is enabled to approach the box hinge block 13, the sealing door 3 is gradually abutted, the sealing door 3 is enabled to be tightly attached to the peripheral end face of the upper opening of the sealing box 1, the hand wheel 13 is stopped to rotate when the sealing door 3 is abutted to a certain degree with the sealing box 1, and the sealing effect of the sealing box 1 is effectively guaranteed; when opening sealing door 3, the manual work rotates hand wheel 13 and makes hand wheel 13 keep away from to articulated piece 13, stops to rotate hand wheel 13 when keeping away from to the certain degree, takes out threaded rod 14 joint groove 32, directly opens sealing door 3.
Elevating system 6 includes fixed block 61, lead screw 62, No. two driving motor 63, lifter plate 64, rolling disc 65 and driven lever 66, the symmetry is provided with fixed block 61 on being close to the front and back lateral wall of middle part position in the seal box 1, is provided with lead screw 62 with normal running fit's mode between the last lateral wall of fixed block 61 and seal box 1, four turnings of lifter plate 64 correspond the spiro union on lead screw 62, the middle part of lifter plate 64 is provided with rolling disc 65 with normal running fit's mode, evenly just be provided with driven lever 66 with normal running connection's mode along its circumference on the rolling disc 65, the quantity and the position of driven lever 66 all correspond with dwang 56, and driven lever 66 and dwang 56 sliding connection. During specific work, the second driving motor 63 works, the output shaft of the second driving motor 63 rotates to drive the screw rod 62 to rotate, the lifting plate 64 is driven to move downwards through the thread transmission of the screw rod 62, the rotating rod 56 in the driven rod 66 is gradually pulled out, the placing mechanism 7 is driven to move downwards by the driven rod 66, and when a culture dish on the placing mechanism 7 is completely immersed in the disinfection solution, the second driving motor 63 stops working.
The rotating mechanism 5 comprises a rotating fluted disc 51, a first driving motor 52, a driving gear 53, a fixed shaft 54, a fixed gear 55, a rotating rod 56 and a rotating wheel 57, the rotating fluted disc 51 is connected to the top of the sealing box 1 in a rotating fit manner, the rotating fluted disc 51 is of a hollow structure, the first driving motor 52 is arranged on the upper end surface of the sealing box 1, the driving gear 53 is arranged at one end, entering the sealing box 1, of an output shaft of the first driving motor 52, the driving gear 53 is meshed with the rotating fluted disc 51, the fixed shaft 54 is arranged at the top of the sealing box 1, the axis of the fixed shaft 54 is on the same line with the axis of the rotating fluted disc 51, the fixed gear 55 is arranged at one end, entering the rotating fluted disc 51, of the fixed shaft 54, the rotating rod 56 is uniformly arranged on the lower end surface of the rotating fluted disc 51 along the circumferential direction and in a rotating connection manner, and the rotating wheel 57, the rotating gear 57 and the fixed gear 55 are engaged with each other. During the operation, the first driving motor 52 is operated, the output shaft of the first driving motor 52 intermittently rotates at a slow speed to drive the driving gear 53 to intermittently rotate, the driving gear 53 intermittently rotates to drive the rotating fluted disc 51 to intermittently rotate, the rotating rod 56 intermittently revolves around the axis of the rotating fluted disc 51 along with the rotating fluted disc 51, meanwhile, the rotating wheel 57 and the fixed gear 55 are driven by the rotating fluted disc 51 to intermittently rotate relatively, because the fixed gear 55 is fixed, the rotating rod 56 is driven by the rotating fluted disc 51 to intermittently rotate, the rotating rod 56 intermittently rotates to drive the driven rod 66 to intermittently rotate, and the driven rod 66 drives the rotating disc 65 to intermittently rotate, so that the placing mechanism 7 on the driven rod 66 intermittently rotates while intermittently revolving around the axis of the rotating fluted disc 51, and the placing mechanism 7 and the culture dishes thereon stir the disinfection solution in the sealed box 1, so that the culture dish can be contacted with the disinfection solution fully to wash and disinfect, and further, the stains on the culture dish are washed away.
The cleaning mechanism 8 comprises an installation box 81, an electric push rod 82, a sliding plate 83, a telescopic shaft 84, a cleaning brush 85, a second transmission gear 86 and a third driving motor 87, the installation box 81 is of a box-shaped structure with an opening at the right end, the installation box 81 is installed on the lower side of the left end face of the seal box 1, the electric push rod 82 is installed on the left side wall of the installation box 81, the sliding plate 83 is arranged in the installation box 81 in a sliding mode, the telescopic rod of the electric push rod 82 extends into the installation box 81 and is connected onto the sliding plate 83, the telescopic shaft 84 is arranged on the sliding plate 83 in a rotating matching mode and is uniformly and linearly arranged, the distance between the telescopic shafts 84 is the same as that between the placing rings 711, one end, far away from the sliding plate 83, of the telescopic shaft 84 penetrates through the side wall of the seal box 1 to enter the seal box 1 and is provided, the second transmission gears 86 are meshed with each other in sequence, the third driving motor 87 is arranged on the left side of the mounting plate, and an output shaft of the third driving motor 87 is connected with one telescopic shaft 84 on the uppermost side of the sliding plate 83. When the placing mechanism 7 intermittently rotates while intermittently revolving around the axis of the rotating fluted disc 51, the opening of the culture dish on the placing mechanism 7 is opposite to the cleaning brush 85 when each intermittent stop is performed, at the moment, the electric push rod 82 and the third driving motor 87 work, the telescopic rod of the electric push rod 82 extends to drive the sliding plate 83 to move to the right side in the installation box 81, so that the telescopic shaft 84 moves inwards in a sealing way, when the telescopic shaft 84 drives the cleaning brush 85 to extend into the inner side of the culture dish, the electric push rod 82 stops working, meanwhile, the output shaft of the third driving motor 87 rotates to drive the second transmission gear 86 to rotate, mutual transmission between the second transmission gear 86 enables the telescopic shaft 84 to rotate when moving into the sealing box 1, when the cleaning brush 85 extends into the inner side of the culture dish, the inner wall of the culture dish is further cleaned through the rotation of the cleaning brush 85, the cleaning effect of the culture dish is improved, after cleaning is completed, the electric push rod 82 works, the telescopic rod shrinkage through the electric push rod 82 enables the cleaning brush 85 to be withdrawn from the culture dish and far away from the placing mechanism 7, and the culture dish on the placing mechanism 7 through which sequential intermittent stop passes is circulated for multiple times of cleaning, so that the culture dish can be cleaned more thoroughly.
When the culture dish is disinfected and cleaned in the disinfection solution for a certain time, the first driving motor 52 stops working, the second driving motor 63 starts working, the output shaft of the second driving motor 63 rotates to drive the screw rod 62 to rotate, the lifting plate 64 drives the lifting plate to move upwards through the screw transmission of the screw rod 62, the rotating rod 56 gradually enters the driven rod 66, the placing mechanism 7 is driven by the driven rod 66 to move upwards, when the placing mechanism 7 rises to a certain position and the culture dish corresponds to the disinfection lamp 4, the second driving motor 63 stops working, the disinfection lamp 4 is opened, and the disinfection lamp 4 emits ultraviolet light; at the moment, the first driving motor 52 works, the output shaft of the first driving motor 52 continuously and rapidly rotates to enable the placing mechanism 7 and the culture dish on the placing mechanism to revolve and rotate around the axis of the rotary fluted disc 51, so that the culture dish uniformly irradiates ultraviolet rays, irradiation dead angles are prevented from being generated, bacteria and viruses on the culture dish can be killed through irradiation of the ultraviolet rays, the disinfection effect is greatly improved, and water on the culture dish is thrown out when the culture dish rotates along with the placing mechanism 7; after the culture dish is irradiated by ultraviolet rays for a proper time, bacteria and viruses on the culture dish are killed. The sealing door 3 is opened, and the first limiting rod 74 is pulled to sequentially take out the culture dishes from the placing ring 711.
The left side wall of the seal box 1 is provided with a vacuum pump 12, an air suction port of the vacuum pump 12 is communicated with the interior of the seal box 1, and an air inlet valve 13 is arranged on the side wall of the seal box 1 on one side of the vacuum pump 12. During specific work, when the culture dish carries out ultraviolet irradiation, vacuum pump 12 work, and vacuum pump 12 is taken out into the vacuum in with seal box 1 for aerobic bacteria on the culture dish is killed, through the living condition that reduces the bacterium in order to increase the disinfection effect to the culture dish, before opening sealing door 3, vacuum pump 12 stop work, slowly opens air inlet valve 13, makes in the air admission seal box 1, prevents that sealing door 3 from being difficult to open.
And a sealing gasket 21 is embedded on the sealing box 1 around the outer side of the inlet and outlet 2. The sealing performance between the sealing door 3 and the sealing box 1 is increased.
A water supply valve 15 and a water discharge valve 16 are arranged on the lower side of the right side wall of the seal box 1. A certain amount of the sterilizing solution is introduced into the sealing box 1 by opening the water supply valve 15, the water supply valve 15 is closed after the input of the sterilizing solution is completed, and when the sterilizing solution is used to a certain extent, the sterilizing solution is discharged through the drain valve 16 to be replaced.
When the cell culture dish sterilization and disinfection equipment is used for sterilizing and disinfecting the cell culture dish, the method comprises the following steps:
s1, placing a culture dish: placing and fixing the culture dish to be sterilized and disinfected through a placing mechanism 7, and closing the sealing door 3;
s2, washing a culture dish: the lifting mechanism 6 drives the placing mechanism 7 to descend into the disinfection solution, the culture dish is soaked in the disinfection solution for disinfection, and meanwhile, the rotating mechanism 5 drives the driven rod 66 to revolve and rotate around the axial lead of the rotating fluted disc 51, so that the placing mechanism 7 is driven to stir in the disinfection solution, and meanwhile, the placing mechanism 7 is matched with the cleaning mechanism 8 to clean the inner wall of the culture dish;
s3, ultraviolet sterilization: the lifting mechanism 6 drives the placing mechanism 7 to ascend to the upper side of the sealing box 1, the disinfection lamp 4 emits ultraviolet light to irradiate, sterilize and disinfect the culture dish, meanwhile, the rotating mechanism 5 drives the placing mechanism 7 to rotate so that the culture dish uniformly irradiates ultraviolet, the culture dish throws out moisture in rotation, and ultraviolet irradiation is completed after a certain time;
s4, vacuum sterilization: at the same time of S3, vacuumizing the air in the sealed box 1 for sterilization;
s5, taking out the culture dish: after the sterilization is completed, the sealing door 3 is opened, and the culture dish is taken out from the placing mechanism 7.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. The utility model provides a cell culture dish disinfection method that disinfects, this cell culture dish disinfection method that disinfects adopts following disinfection equipment, this cell culture dish disinfection equipment that disinfects includes seal box (1), import and export (2), sealing door (3), sterilamp (4), rotary mechanism (5), elevating system (6), placement machine structure (7) and wiper mechanism (8), its characterized in that: the sealing box (1) is of a box body structure, an inlet and an outlet (2) which are communicated from inside to outside are formed in the upper side of the right end face of the sealing box (1), a sealing door (3) is hinged to the outer wall of the sealing box (1) at the position of the inlet and the outlet (2), a disinfection lamp (4) is arranged on the inner side wall of the upper side of the sealing box (1), a rotating mechanism (5) and a lifting mechanism (6) are arranged on the upper side of the sealing box (1), a placing mechanism (7) is arranged on the lifting mechanism (6), and a cleaning mechanism (8) is arranged on the lower side of the sealing box (1; wherein:
the rotating mechanism (5) comprises a rotating fluted disc (51), a first driving motor (52), a driving gear (53), a fixed shaft (54), a fixed gear (55), a rotating rod (56) and a rotating wheel (57), wherein the rotating fluted disc (51) is connected to the top in the sealing box (1) in a rotating fit mode, the rotating fluted disc (51) is of a hollow structure, the first driving motor (52) is arranged on the upper end surface of the sealing box (1), the driving gear (53) is arranged at one end, entering the sealing box (1), of an output shaft of the first driving motor (52), the driving gear (53) is meshed with the rotating fluted disc (51), the fixed shaft (54) is arranged at the top in the sealing box (1), the axis of the fixed shaft (54) and the axis of the rotating fluted disc (51) are on the same line, the fixed gear (55) is arranged at one end, entering the rotating fluted disc (51), of the fixed shaft (54), the lower end surface of the rotary fluted disc (51) is uniformly provided with a rotating rod (56) in a rotating connection mode along the circumferential direction, one end of the upper end of the rotating rod (56) entering the rotary fluted disc (51) is provided with a rotating wheel (57), and the rotating wheel (57) is meshed with the fixed gear (55);
the lifting mechanism (6) comprises a fixing block (61), a screw rod (62), a second driving motor (63), a lifting plate (64), a rotating disc (65) and a driven rod (66), wherein the fixing block (61) is symmetrically arranged on the front side wall and the rear side wall of the sealing box (1) close to the middle position, the screw rod (62) is arranged between the fixing block (61) and the upper side wall of the sealing box (1) in a rotating fit mode, four corners of the lifting plate (64) are correspondingly screwed on the screw rod (62), the rotating disc (65) is arranged in the middle of the lifting plate (64) in a rotating fit mode, the driven rods (66) are uniformly arranged on the rotating disc (65) along the circumferential direction of the rotating disc in a rotating connection mode, the number and the positions of the driven rods (66) correspond to the rotating rod (56), and the driven rods (66) are in sliding connection with the rotating rod (56;
the placing mechanism (7) comprises a first mounting plate (71), a second mounting plate (72), an arc sliding groove (73), a first limiting rod (74), a toggle gear (75), a connecting rod (76), a first transmission gear (77), a contraction spring (78), a second limiting rod (79), a fixed rod (710) and a placing ring (711), wherein the first mounting plate (71) is arranged at the lower end of the driven rod (66), the second mounting plate (72) is arranged on the driven rod (66) on the upper side of the first mounting plate (71), the second mounting plate (72) is of a hollow structure, the arc sliding grooves (73) are symmetrically formed in one sides, opposite to the first mounting plate (71) and the second mounting plate (72), far away from the driven rod (66), the first limiting rod (74) is arranged between the two arc sliding grooves (73), opposite to the second mounting plate (72), of the first mounting plate (71), toggle gears (75) are symmetrically arranged in the second mounting plate (72) in a rotation fit manner, the central axis of the toggle gear (75) and the arc-shaped circle center of the arc-shaped sliding chute (73) are on the same straight line, the upper end of a first limiting rod (74) extends into the second mounting plate (72) and is connected to the side wall of the toggle gear (75) through a connecting rod (76), one end of the upper end of the first limiting rod (74) extending into the second mounting plate (72) is connected through a contraction spring (78), first transmission gears (77) are symmetrically arranged in the second mounting plate (72) between the toggle teeth in a rotation fit manner, the first transmission gears (77) are mutually meshed, the toggle gear (75) is mutually meshed with one first transmission gear (77) close to the toggle gear, second limiting rods (79) are symmetrically arranged between the opposite surfaces of the first mounting plate (71) and the second mounting plate (72) and at positions close to the driven rod (66), fixing rods (710) are symmetrically arranged between opposite surfaces of a first mounting plate (71) and a second mounting plate (72) between the first limiting rod (74) and the second limiting rod (79), and a placing ring (711) is linearly and uniformly arranged between the two fixing rods (710) from top to bottom;
when the cell culture dish sterilization and disinfection equipment is used for sterilizing and disinfecting the cell culture dish, the method comprises the following steps:
s1, placing a culture dish: placing and fixing the culture dish to be sterilized and disinfected through a placing mechanism (7), and closing the sealing door (3);
s2, washing a culture dish: the lifting mechanism (6) drives the placing mechanism (7) to descend into the disinfection solution, the culture dish is soaked in the disinfection solution for disinfection, and meanwhile, the rotating mechanism (5) drives the driven rod (66) to revolve and rotate around the axial lead of the rotating fluted disc (51), so that the placing mechanism (7) is driven to stir in the disinfection solution, and meanwhile, the placing mechanism and the cleaning mechanism (8) are matched to clean the inner wall of the culture dish;
s3, ultraviolet sterilization: the lifting mechanism (6) drives the placing mechanism (7) to rise to the upper side of the sealing box (1), the culture dish is irradiated and sterilized by ultraviolet light emitted by the sterilizing lamp (4), meanwhile, the rotating mechanism (5) drives the placing mechanism (7) to rotate so that the culture dish uniformly irradiates ultraviolet rays, the culture dish throws out moisture of the culture dish during rotation, and ultraviolet irradiation is completed after a certain time;
s4, vacuum sterilization: vacuumizing the air in the sealed box (1) for sterilization while S3 is carried out;
s5, taking out the culture dish: after the disinfection is finished, the sealing door (3) is opened, and the culture dish is taken out from the placing mechanism (7).
2. The method for sterilizing and disinfecting a cell culture dish according to claim 2, wherein the method comprises the following steps: the cleaning mechanism (8) comprises an installation box (81), an electric push rod (82), a sliding plate (83), a telescopic shaft (84), a cleaning brush (85), a second transmission gear (86) and a third driving motor (87), the installation box (81) is of a box-shaped structure with an opening at the right end, the installation box (81) is installed on the lower side of the left end face of the sealing box (1), the electric push rod (82) is installed on the left side wall of the installation box (81), the sliding plate (83) is arranged in the installation box (81) in a sliding mode, a telescopic rod of the electric push rod (82) extends into the installation box (81) and is connected onto the sliding plate (83), the telescopic shaft (84) is arranged on the sliding plate (83) in a rotating matching mode and in a uniform straight line mode, the distance between the telescopic shafts (84) is the same as the distance between the placing rings (711), one end, far away from the sliding plate (83), of the telescopic shaft (84), penetrates through the side wall of the sealing box (1 A second transmission gear (86) is arranged on one side, close to the sliding plate (83), of the telescopic shaft (84), the second transmission gears (86) are sequentially meshed with each other, a third driving motor (87) is arranged on the left side of the mounting plate, and an output shaft of the third driving motor (87) is connected with the telescopic shaft (84) on the uppermost side of the sliding plate (83).
3. The method for sterilizing and disinfecting a cell culture dish according to claim 1, wherein the method comprises the following steps: the vacuum pump (12) is arranged on the left side wall of the seal box (1), an air suction port of the vacuum pump (12) is communicated with the interior of the seal box (1), and an air inlet valve (13) is arranged on the side wall of the seal box (1) on one side of the vacuum pump (12).
4. The method for sterilizing and disinfecting a cell culture dish according to claim 1, wherein the method comprises the following steps: sealing door (3) keep away from with seal box (1) articulated one end be provided with joint piece (31), seted up joint groove (32) on joint piece (31), be provided with articulated piece (13) on seal box (1) lateral wall with joint piece (31) with one side, threaded rod (14) one end articulates at articulated piece (13), the other end spiro union of threaded rod (14) has hand wheel (13), and threaded rod (14) and joint groove (32) cooperation.
5. The method for sterilizing and disinfecting a cell culture dish according to claim 1, wherein the method comprises the following steps: and a sealing gasket (21) is embedded on the sealing box (1) at the periphery of the outer side of the inlet and outlet (2).
6. The method for sterilizing and disinfecting a cell culture dish according to claim 1, wherein the method comprises the following steps: and a water supply valve (15) and a water drainage valve (16) are arranged on the lower side of the right side wall of the seal box (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110110504.9A CN112741918A (en) | 2021-01-27 | 2021-01-27 | Cell culture dish sterilization and disinfection method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110110504.9A CN112741918A (en) | 2021-01-27 | 2021-01-27 | Cell culture dish sterilization and disinfection method |
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| CN112741918A true CN112741918A (en) | 2021-05-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110110504.9A Withdrawn CN112741918A (en) | 2021-01-27 | 2021-01-27 | Cell culture dish sterilization and disinfection method |
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| CN (1) | CN112741918A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114642759A (en) * | 2022-04-15 | 2022-06-21 | 蒋金洲 | Incubator degassing unit |
| CN114949283A (en) * | 2022-07-15 | 2022-08-30 | 南京善台引生物科技有限公司 | Stem cell culture dish disinfection system and disinfection method |
-
2021
- 2021-01-27 CN CN202110110504.9A patent/CN112741918A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114642759A (en) * | 2022-04-15 | 2022-06-21 | 蒋金洲 | Incubator degassing unit |
| CN114642759B (en) * | 2022-04-15 | 2023-11-24 | 孙健春 | Incubator degassing unit |
| CN114949283A (en) * | 2022-07-15 | 2022-08-30 | 南京善台引生物科技有限公司 | Stem cell culture dish disinfection system and disinfection method |
| CN114949283B (en) * | 2022-07-15 | 2024-05-31 | 河南中旭再生医学研究院有限公司 | Stem cell culture dish disinfection system and disinfection method |
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Application publication date: 20210504 |