CN112730868B - Sample injection system for continuous flow analyzer - Google Patents
Sample injection system for continuous flow analyzer Download PDFInfo
- Publication number
- CN112730868B CN112730868B CN202011574573.7A CN202011574573A CN112730868B CN 112730868 B CN112730868 B CN 112730868B CN 202011574573 A CN202011574573 A CN 202011574573A CN 112730868 B CN112730868 B CN 112730868B
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- Prior art keywords
- bubble
- pipe fitting
- pressing pipe
- sample
- gas
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- 238000002347 injection Methods 0.000 title claims abstract description 14
- 239000007924 injection Substances 0.000 title claims abstract description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 30
- 238000002955 isolation Methods 0.000 claims abstract description 8
- 238000005192 partition Methods 0.000 claims abstract description 3
- 230000005540 biological transmission Effects 0.000 claims description 2
- 230000000903 blocking effect Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 230000005587 bubbling Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The application discloses a sample injection system for a continuous flow analyzer, which comprises a sample pipeline, a reagent pipeline and a bubble generation mechanism, wherein the bubble generation mechanism comprises a plurality of bubble hoses and a bubble isolation device; the bubble partition device comprises a fixed seat, and a first pressing pipe fitting and a second pressing pipe fitting which are arranged on the fixed seat, wherein the first pressing pipe fitting and the second pressing pipe fitting are distributed along the cross section direction of the hose, and the first pressing pipe fitting and the second pressing pipe fitting are driven by a driving mechanism arranged in the fixed seat respectively, so that the first pressing pipe fitting and the second pressing pipe fitting alternately press the bubble hose in sequence. The application can separate the reagent from the sample, thereby preventing the reagent and the sample between two adjacent sections from affecting each other, and enabling the reagent and the sample to fully react.
Description
Technical Field
The present application relates to a continuous flow analyzer, and more particularly, to a sample injection system for a continuous flow analyzer.
Background
In a Continuous Flow Analyzer (CFA), reagents and samples are pumped into a reaction line by a peristaltic pump during an on-line analysis process, so that the reagents and samples react in the reaction line, but since the reagents and samples in the reaction line of the flow analyzer are in a continuous flow state, the reagents and samples react while flowing, and in this process, since the flows of the reagents and samples are continuous, the reagents and samples located upstream are easily affected by the reagents and samples located downstream, so that the reagents and samples cannot sufficiently react, thereby affecting the accuracy of the continuous flow analyzer.
Disclosure of Invention
In order to solve the above-mentioned shortcomings in the prior art, an object of the present application is to provide a sample injection system for a continuous flow analyzer, which can separate a reagent from a sample, thereby preventing the reagent and the sample between two adjacent sections from affecting each other, and allowing the reagent and the sample to react sufficiently.
The technical scheme adopted for solving the technical problems is as follows: a sample injection system for a continuous flow analyzer comprises a sample pipeline, a reagent pipeline and a bubble generation mechanism, wherein the reagent pipeline and the bubble generation mechanism are respectively connected with the sample pipeline, and the bubble generation mechanism is arranged at the upstream of the reagent pipeline;
the bubble generation mechanism comprises a plurality of bubble hoses and bubble isolation devices, one ends of the bubble hoses are connected with an air source, the other ends of the bubble hoses are connected with the sample pipeline, and the middle parts of the bubble hoses are arranged on the bubble isolation devices, so that gas in the bubble hoses is isolated by the bubble isolation devices;
the bubble partition device comprises a fixing seat, and a first pressing pipe fitting and a second pressing pipe fitting which are arranged on the fixing seat, wherein the first pressing pipe fitting and the second pressing pipe fitting are distributed along the cross section direction of the hose, and the first pressing pipe fitting and the second pressing pipe fitting are driven by a driving mechanism arranged in the fixing seat respectively, so that the first pressing pipe fitting and the second pressing pipe fitting alternately press the bubble hose in sequence.
Optionally, the driving mechanism includes a rotating shaft and two protrusions respectively and fixedly installed on the rotating shaft, and the two protrusions are respectively connected with the first pipe pressing piece and the second pipe pressing piece in a transmission manner;
the phase difference between the two protrusions is 180 °.
Optionally, the first pipe pressing piece comprises a connecting rod, the connecting rod is slidably connected to the fixing base, a pressing rod is fixedly arranged at the top of the connecting rod, and the bottom of the connecting rod is matched with the corresponding protrusion.
Optionally, a return spring is further sleeved on the connecting rod.
Optionally, a slit for clamping the bubble hose is formed in the fixing base, and the bubble hose is arranged in the slit.
Optionally, the bubble hose is further connected with an air pressure adjusting device, the air pressure adjusting device comprises a valve body, a plurality of air outlets and an air inlet, wherein the air outlets and the air inlets are arranged in the valve body, one air outlet is connected with an adjusting knob, and the air outlet air pressure of the other air outlets is adjusted through the adjusting knob.
Optionally, a gas outlet provided with the adjusting knob is communicated with the outside, and the other gas outlets are connected with the bubble hose;
the gas inlet is connected to a gas source by a conduit.
Optionally, the air source is compressed air.
By adopting the technical scheme, the bubble is injected into the reaction pipeline through the bubble generating mechanism, so that the reagent and the sample in the sample pipeline can be effectively separated, the mutual influence between two adjacent sections of reagents and the sample is prevented, and favorable conditions are provided for the full reaction of the reagents and the sample. In addition, the bubble generation mechanism can enable bubbles to enter the sample pipeline in a pulse mode through the bubble isolation device, and uniformity of the bubbles is guaranteed.
Drawings
FIG. 1 is a schematic diagram of a piping connection of the present application;
FIG. 2 is a schematic view showing a state of the bubble blocking device according to the present application;
FIG. 3 is a second schematic view of the bubble blocking device of the present application;
FIG. 4 is a three-schematic view of the state of the bubble blocking device of the present application;
fig. 5 is a schematic structural view of the air pressure adjusting device of the present application.
Detailed Description
The application is described in further detail below with reference to the drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the application and are not limiting of the application. It should be noted that, for convenience of description, only the portions related to the application are shown in the drawings.
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The application will be described in detail below with reference to the drawings in connection with embodiments.
As shown in fig. 1, the application discloses a sample injection system for a continuous flow analyzer, which comprises a sample pipeline 100, a reagent pipeline 200 and a bubble generation mechanism, wherein the reagent pipeline 200 and the bubble generation mechanism are respectively connected with the sample pipeline 100. The sample pipeline 100 is used for introducing a sample and is used as a reaction pipeline, the reagent pipeline 200 is used for introducing a reaction reagent into the sample pipeline 100, and the bubble generating mechanism is used for bubbling into the sample pipeline 100 so as to enable the sample and the reagent mixed solution in the sample pipeline 100 to have a blocking effect and prevent the adjacent sample and the reagent mixed solution from interfering with each other.
In the application, the bubble generating mechanism comprises a plurality of bubble hoses 300 and a bubble blocking device 400, one end of each bubble hose 300 is connected with an air source, the other end of each bubble hose 300 is connected with a sample pipeline 100, the middle part of each bubble hose 300 is arranged on the bubble blocking device 400, so that gas in each bubble hose 300 is blocked by the bubble blocking device 400, and the gas enters the sample pipeline 100 in a pulse mode.
In the present application, the bubble blocking device 400 includes a fixing base 410, and a first pressing pipe 420 and a second pressing pipe 430 disposed on the fixing base 410, wherein the fixing base 410 is fixedly installed inside a housing of the continuous flow analyzer, the first pressing pipe 420 and the second pressing pipe 430 are distributed along a cross-sectional direction of the bubble hose 300, and the first pressing pipe 420 and the second pressing pipe 430 are respectively driven by a driving mechanism 500 installed in the fixing base 410, so that the first pressing pipe 420 and the second pressing pipe 430 alternately press the bubble hose 300 in sequence.
In the present application, as shown in fig. 2, the driving mechanism 500 includes a rotation shaft 510 and two protrusions 520 fixedly installed on the rotation shaft, respectively, and the two protrusions 520 are in driving connection with the first pressing pipe 420 and the second pressing pipe 430, respectively. The phase difference between the two protrusions 520 is 180 °, so that the two protrusions 520 work to make the first pressing pipe 420 and the second pressing pipe 430 have three different matching states, namely, the first pressing pipe 420 is lifted, the second pressing pipe 430 keeps pressing the air bubble hose 300, the second pressing pipe 420 and the second pressing pipe 430 simultaneously keep pressing the air bubble hose 300, and the third pressing pipe 420 keeps pressing the air bubble hose 300, and the second pressing pipe 430 is lifted. In state one, as shown in fig. 2, gas is forced into the bubble hose 300. As shown in fig. 3, in the second state, the gas is in a pressure-maintaining state within the bubble hose 300. In state three, as shown in fig. 4, the air pressure in the air bubble hose 300 is greater than the hydraulic pressure in the sample line 100, and air is forced into the sample line 100, thereby generating continuously spaced and uniformly distributed air bubbles within the sample line 100. During operation of the continuous flow analyzer, the bubble blocking device 400 is continuously cycled from state one to state two to state three by the drive mechanism 500.
In the present application, as shown in fig. 2, the first pressing pipe 420 includes a connection rod 421, the connection rod 421 is slidably connected to the fixing base 410, a pressing rod 422 is fixedly installed at the top of the connection rod 421, the bottom of the connection rod 421 is matched with the corresponding protrusion 520, and the second pressing pipe 430 has the same structure as the first pressing pipe 420. In the present application, since the pressing rod 422 is connected to the top of the connection rod 421, the pressing rod 422 prevents the connection rod 421 from continuing to move downward when the pressing rod 422 forms a pressed state on the bubble hose 300, and thus the pressing rod 422 also serves as a stopper of the connection rod 421. A return spring 423 is further sleeved on the connecting rod 421, and the connecting rod 421 is driven to move downwards by the spring 423. Specifically, a blocking seat 424 is fixed at the bottom of the connecting rod 421, and the blocking seat 424 can be used as a supporting base of the return spring 423 on one hand, and can be used as a jacking top seat of the protrusion 520 on the other hand.
In the present application, a slit 440 for clamping the bubble hose 300 may be provided at the fixing base 410, the size of the slit 440 being adapted to the outer diameter of the bubble hose 300, and the bubble hose 300 is clamped in the slit 440 when the bubble hose 300 is fixed.
In the present application, as shown in fig. 5, the air pressure adjusting device 600 is further provided in the holder 410, and the air pressure adjusting device 600 includes a valve body 610, and a plurality of air outlets 620 and an air inlet 630 provided in the valve body, wherein one of the air outlets 620 is connected with an adjusting knob 640, and the air outlets 620 provided with the adjusting knob 640 are communicated with the outside, the remaining air outlets 620 are connected with the bubble hose 300, and the air inlet 630 is connected to an air source through a pipe, and in the present application, the air source may employ an air non-reactive with a reagent and a sample, such as compressed air. The present application can adjust the outlet flow rate of one of the gas outlets 620 by rotating the adjusting knob 640, thereby realizing the adjustment of the outlet gas pressure of the remaining gas outlets 620.
The above description is only illustrative of the preferred embodiments of the present application and of the principles of the technology employed. It will be appreciated by persons skilled in the art that the scope of the application referred to in the present application is not limited to the specific combinations of the technical features described above, but also covers other technical features formed by any combination of the technical features described above or their equivalents without departing from the inventive concept. Such as the above-mentioned features and the technical features disclosed in the present application (but not limited to) having similar functions are replaced with each other.
Other technical features besides those described in the specification are known to those skilled in the art, and are not described herein in detail to highlight the innovative features of the present application.
Claims (7)
1. The sample injection system for the continuous flow analyzer is characterized by comprising a sample pipeline, a reagent pipeline and a bubble generation mechanism, wherein the reagent pipeline and the bubble generation mechanism are respectively connected with the sample pipeline;
the bubble generation mechanism comprises a plurality of bubble hoses and bubble isolation devices, one ends of the bubble hoses are connected with an air source, the other ends of the bubble hoses are connected with the sample pipeline, and the middle parts of the bubble hoses are arranged on the bubble isolation devices, so that gas in the bubble hoses is isolated by the bubble isolation devices;
the bubble partition device comprises a fixed seat, and a first pressing pipe fitting and a second pressing pipe fitting which are arranged on the fixed seat, wherein the first pressing pipe fitting and the second pressing pipe fitting are distributed along the cross section direction of the bubble hose, and the first pressing pipe fitting and the second pressing pipe fitting are respectively driven by a driving mechanism arranged in the fixed seat, so that the first pressing pipe fitting and the second pressing pipe fitting alternately press the bubble hose in sequence; the driving mechanism comprises a rotating shaft and two protrusions which are respectively and fixedly arranged on the rotating shaft, the two protrusions are respectively connected with the first pressing pipe fitting and the second pressing pipe fitting in a transmission mode, and the phase difference between the two protrusions is 180 degrees.
2. The sample injection system for a continuous flow analyzer of claim 1, wherein the first pressure tube comprises a connecting rod slidably connected in the fixed seat, a pressure rod is fixedly mounted on the top of the connecting rod, and the bottom of the connecting rod is matched with the corresponding protrusion.
3. The sample injection system for a continuous flow analyzer of claim 2, wherein the connecting rod is further sleeved with a return spring.
4. The sample injection system for a continuous flow analyzer of claim 3, wherein the holder is provided with a slit for clamping a bubble hose, and the bubble hose is disposed in the slit.
5. The sample injection system for a continuous flow analyzer of claim 4, wherein the bubble hose is further connected with a gas pressure adjusting device, the gas pressure adjusting device comprises a valve body, a plurality of gas outlets and a gas inlet, wherein the gas outlets and the gas inlet are arranged in the valve body, one gas outlet is connected with an adjusting knob, and the gas outlet pressure of the rest gas outlets is adjusted through the adjusting knob.
6. The sample injection system for a continuous flow analyzer of claim 5, wherein a gas outlet to which the adjustment knob is mounted is communicated with the outside, and the remaining gas outlets are connected to the bubble hose;
the gas inlet is connected to a gas source by a conduit.
7. The sample injection system for a continuous flow analyzer of claim 6, wherein the gas source is compressed air.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011574573.7A CN112730868B (en) | 2020-12-26 | 2020-12-26 | Sample injection system for continuous flow analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011574573.7A CN112730868B (en) | 2020-12-26 | 2020-12-26 | Sample injection system for continuous flow analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112730868A CN112730868A (en) | 2021-04-30 |
| CN112730868B true CN112730868B (en) | 2023-11-07 |
Family
ID=75606068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011574573.7A Active CN112730868B (en) | 2020-12-26 | 2020-12-26 | Sample injection system for continuous flow analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112730868B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6610499B1 (en) * | 2000-08-31 | 2003-08-26 | The Regents Of The University Of California | Capillary array and related methods |
| CN101906378A (en) * | 2010-07-05 | 2010-12-08 | 博奥生物有限公司 | Bubble micro valve and bubble micro valve-based micro-fluidic chip |
| CN102149812A (en) * | 2008-02-21 | 2011-08-10 | 埃凡特拉生物科技公司 | Assays based on liquid flow over arrays |
| CN103837462A (en) * | 2014-03-03 | 2014-06-04 | 中国科学院苏州生物医学工程技术研究所 | Miniature flow cytometer fluid path system |
| CN107064529A (en) * | 2017-04-06 | 2017-08-18 | 亚智***科技(苏州)有限公司 | A kind of automatic analysing apparatus and automatic analysis method |
| CN110658140A (en) * | 2019-09-29 | 2020-01-07 | 北京海光仪器有限公司 | Chemical analysis system for total phosphorus |
| CN110658139A (en) * | 2019-09-29 | 2020-01-07 | 北京海光仪器有限公司 | Permanganate index analysis system |
| CN110658138A (en) * | 2019-09-29 | 2020-01-07 | 北京海光仪器有限公司 | Sulfide analysis system |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030013200A1 (en) * | 2001-07-12 | 2003-01-16 | Su-Cheng Pai | Liquid sample take-up device |
-
2020
- 2020-12-26 CN CN202011574573.7A patent/CN112730868B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6610499B1 (en) * | 2000-08-31 | 2003-08-26 | The Regents Of The University Of California | Capillary array and related methods |
| CN102149812A (en) * | 2008-02-21 | 2011-08-10 | 埃凡特拉生物科技公司 | Assays based on liquid flow over arrays |
| CN101906378A (en) * | 2010-07-05 | 2010-12-08 | 博奥生物有限公司 | Bubble micro valve and bubble micro valve-based micro-fluidic chip |
| CN103837462A (en) * | 2014-03-03 | 2014-06-04 | 中国科学院苏州生物医学工程技术研究所 | Miniature flow cytometer fluid path system |
| CN107064529A (en) * | 2017-04-06 | 2017-08-18 | 亚智***科技(苏州)有限公司 | A kind of automatic analysing apparatus and automatic analysis method |
| CN110658140A (en) * | 2019-09-29 | 2020-01-07 | 北京海光仪器有限公司 | Chemical analysis system for total phosphorus |
| CN110658139A (en) * | 2019-09-29 | 2020-01-07 | 北京海光仪器有限公司 | Permanganate index analysis system |
| CN110658138A (en) * | 2019-09-29 | 2020-01-07 | 北京海光仪器有限公司 | Sulfide analysis system |
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| Publication number | Publication date |
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| CN112730868A (en) | 2021-04-30 |
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