CN112697780B - Mercury ion colorimetric detection method based on osmium nanoparticle oxidase activity - Google Patents
Mercury ion colorimetric detection method based on osmium nanoparticle oxidase activity Download PDFInfo
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Abstract
The invention discloses a mercury ion colorimetric detection method based on osmium nanoparticle oxidase activity. According to the invention, the enhancement of the activity of oxidase is simulated by utilizing the specific interaction of the heparin modified osmium nanoparticles and mercury ions, the heparin modified osmium nanoparticles sensitized by mercury ions catalyze and oxidize 3,3', 5' -tetramethyl benzidine hydrochloride to develop color, along with the increase of the content of mercury ions, the absorption value of a color development system at the position of the maximum absorption wavelength of 600 nm is increased, the detection linear range of mercury ions is 3-40 mu M, the detection sensitivity is high, the detection limit of observing color change by naked eyes is 3 mu M, and the detection limit of spectrophotometry detection is 220 nM. The osmium nanoparticles used for detection are simple and easy to prepare, have good stability, can realize the visualized and convenient analysis of mercury ions by the mercury ion detection method constructed based on the material, and have the advantages of simple and convenient operation, short detection time, high sensitivity, strong specificity and the like, and are easy to popularize and use.
Description
Technical Field
The invention provides a rapid colorimetric method for detecting mercury ions, relates to a colorimetric detection method for mercury ions based on osmium nanoparticle simulated oxidase, and belongs to the technical fields of biochemistry and nanometer.
Background
Mercury can exist in both elemental and mercury compounds in both natural and artificial conditions. Elemental mercury is also known as elemental mercury. Metal mercury poisoning is often caused in the form of mercury vapor. Because mercury vapor has high diffusivity and high fat solubility, the mercury vapor enters alveoli through the respiratory tract and is transported to the whole body through blood circulation. Mercury is discharged from a pollution source into a natural body of water and can immediately interact with various substances in the body of water, including various ions, molecules and complexing ligands dissolved in the water, organic and inorganic particulate matter suspended in the water, water droplet deposits and aquatic organisms.
After the human body ingests mercury ions, the mercury ions can gradually accumulate in brain tissues, so that the brain tissues are damaged when the mercury ions reach a certain amount, and the other part of mercury ions are transferred to kidneys. The clinical manifestations of chronic mercury poisoning are mainly nervous system symptoms, and acute mercury poisoning is manifested by hepatitis, nephritis, proteinuria, hematuria and uremia. At present, conventional detection methods of mercury ions mainly comprise an atomic absorption method, an atomic fluorescence spectrometry, an inductively coupled plasma emission spectrometry and the like. However, these methods are cumbersome to operate, require cumbersome pretreatment, specialized analytical technicians, and expensive instrumentation, and are not conducive to rapid analysis.
Disclosure of Invention
The invention aims to provide a rapid colorimetric method for detecting mercury ions, and relates to a colorimetric detection method for mercury ions based on osmium nanoparticle simulated oxidase. The detection method comprises the steps of utilizing the specific interaction of the heparin modified osmium nanoparticles and mercury ions to simulate the enhancement of oxidase activity, and carrying out catalytic oxidation on 3,3',5,5' -tetramethyl benzidine hydrochloride by the heparin modified osmium nanoparticles after mercury ion sensitization to develop color, wherein the absorption value of a color development system at the position of the maximum absorption wavelength 600 nm is increased along with the increase of the content of the mercury ions. Preliminary judgment according to the color of the color development system solution or quantification by a standard curve drawn according to the absorbance at wavelength 600 nm.
In order to achieve the purpose of the detection method, the invention adopts the following technical scheme:
a mercury ion colorimetric detection method based on osmium nanoparticle simulated oxidase is characterized in that the activity of the simulated oxidase is enhanced after the specific interaction of heparin modified osmium nanoparticles and mercury ions, the heparin modified osmium nanoparticles after sensitization of mercury ions catalyze and oxidize 3,3',5,5' -tetramethyl benzidine hydrochloride to develop color, and the absorption value of a color development system at the position of 600 nm of the maximum absorption wavelength is increased along with the increase of the content of mercury ions; the mercury ion concentration is determined according to the primary judgment of the color of the solution of the color development system or by quantification according to a standard curve drawn according to the absorbance at the wavelength of 600 nm.
The colorimetric detection method for mercury ions based on osmium nanoparticle simulated oxidase is characterized by being capable of primarily judging the concentration of mercury ions according to the color of a color development system solution.
The method for detecting mercury ions by colorimetry based on osmium nanoparticle simulated oxidase is characterized by judging the concentration of the mercury ions according to the absorption value at the maximum absorption wavelength 600 nm of an ultraviolet absorption spectrum.
The mercury ion colorimetric detection method based on osmium nanoparticle simulated oxidase is characterized in that the used heparin modified osmium nanoparticle is prepared by the following method: mixing heparin of 0.1 and g with potassium hexachloroosmium with the concentration of 1 mL and 10 mM, adding 48 mL double distilled water, stirring for 30 minutes under the dark condition, then adding sodium borohydride with the concentration of 1 mL and 0.2M, and continuously stirring for 90 minutes, wherein the color of the solution is changed from light green to light brown, and obtaining the heparin modified osmium nano-particles with the concentration of 50 mL and 38 mg/L.
The method for detecting mercury ions by colorimetry based on osmium nanoparticle simulated oxidase is characterized in that 0.1 mL heparin-modified osmium nanoparticles with the concentration of 38 mg/L are added into 0.4 mL and 3-40 mu M mercury ion sample solutions with different concentrations; then 3300 MuL of phosphate buffer solution with pH value of 6, 20 MuL and mM MuL of 3,3',5,5' -tetramethyl benzidine hydrochloride with concentration of 2 mM are added into the mixed solution, after the mixed solution reacts for different time in a water bath with the temperature of 25 ℃, the absorbance value at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer; when the mercury ions are not contained, the solution is colorless, and the color of the system solution gradually changes into deep blue along with the increase of the concentration of the mercury ions, and the detection limit of naked eyes is 3 mu M; as the content of mercury ions increases, the absorption value of the color development system at the maximum absorption wavelength of 600 nm increases, the color development system and the concentration of mercury ions are in linear relation within the range of 3-40 mu M, and the detection limit is 220 nM; preliminary judgment according to the color of the color development system solution or quantification by a standard curve drawn according to the absorbance at wavelength 600 nm.
The method for detecting mercury ions by colorimetry based on osmium nanoparticle simulated oxidase is characterized in that the concentration of heparin is 2 mg/mL, the pH of a phosphate buffer solution is 6, the reaction temperature in the reaction process is 25 ℃ and the reaction time is 5 minutes.
The invention discloses a mercury ion colorimetric detection method based on osmium nanoparticle simulated oxidase, which is used for detecting the concentration of mercury ions in a water sample and is characterized by comprising the following steps of: taking a tap water sample of 0.4 and mL, and adding heparin-modified osmium nanoparticles with the concentration of 38.1 and mL being 38 mg/L; then 3300 MuL of phosphate buffer solution with pH value of 6, 20 MuL and mM MuL of 3,3',5,5' -tetramethyl benzidine hydrochloride with concentration of 2 mM are added into the mixed solution, after the mixed solution reacts for different time in a water bath with the temperature of 25 ℃, the absorbance value at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer; and calculating the content of mercury ions by combining a standard curve to obtain the content of mercury ions in the water sample.
Specifically, the invention adopts the following technical scheme:
preparation of heparin modified osmium nanoparticles: all glassware used in the process is soaked in aqua regia, thoroughly washed by double distilled water and dried. 0.1 Mixing g heparin with 1 mL potassium hexachloroosmium sulfate with the concentration of 10 mM, adding 48 mL double distilled water, stirring for 30 minutes under the dark condition, then adding 1 mL sodium borohydride with the concentration of 0.2M, and continuously stirring for 90 minutes to obtain 50 mL heparin-modified osmium nanoparticles (with the concentration of 38 mg/L). The solution was light brown and stored at 4 ℃.
(II) a mercury ion detection method:
the prepared heparin-modified osmium nanoparticle according to the technical scheme (I) of 0.1 mL (the concentration is 38 mg/L) is added into a 0.4 mL mercury ion sample solution. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after reaction for 5 min in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. The quantification is carried out according to the preliminary judgment of the color of the solution of the color development system or by a standard curve drawn according to the absorbance at the wavelength 600 nm. The detection limit of visual observation is 3 mu M, and the detection limit of absorbance measurement is 220 nM.
The invention has the advantages that:
(1) The invention mainly utilizes the enhancement of the simulated oxidase activity of the heparin-modified osmium nanoparticles after the specific interaction of the heparin-modified osmium nanoparticles and mercury ions, and the heparin-modified osmium nanoparticles are used for catalyzing and oxidizing 3,3',5,5' -tetramethyl benzidine hydrochloride to develop color, so that the change of the solution color and ultraviolet absorption spectrum characteristics is shown, and the method can be used for detecting the content of mercury ions. The detection work can be completed within 5 minutes and the detection result can be obtained.
(2) The heparin modified osmium nanoparticle is obtained by modifying heparin serving as a stabilizing agent on the surface and reducing potassium hexachloroosmium by sodium borohydride, and the preparation process is simple and quick, and the material is stable and long in storage time.
(3) The invention has the advantages that the dosage of the heparin modified osmium nano particles required by each detection is small, the chromogenic substrate (3, 3',5,5' -tetramethyl biphenyl amine hydrochloride) is common and easy to obtain, and the detection cost is low.
(4) The invention has low requirement on sample treatment, and has very obvious sensitization specificity on mercury ions after being compared with the influence of other 23 ions on oxidase of the heparin modified osmium nano-particles.
(5) The detection linear range of mercury ions is 3-40 mu M, the detection sensitivity is high, the detection limit of color change observed by naked eyes is 3 mu M, and the detection limit of spectrophotometry detection is 220 nM.
In summary, the mercury ion detection method provided by the invention has the advantages of simple detection process, good stability, short detection time, high sensitivity, strong specificity and the like, and is easy to popularize and use.
Drawings
FIG. 1 is a schematic diagram of a colorimetric detection method for mercury ions based on osmium nanoparticle-based mimic oxidase.
Fig. 2 is a photograph of each of the bare osmium nanoparticle and the heparin-modified osmium nanoparticle after being left at room temperature for 10 days, wherein a is the bare osmium nanoparticle and B is the heparin-modified osmium nanoparticle.
FIG. 3 is a graph showing UV absorption spectra of the color development of catalytically oxidized 3,3',5,5' -tetramethylbenzidine hydrochloride after heparin-modified osmium nanoparticles are reacted with mercury ions having final concentrations of 0 and 50. Mu.M, respectively.
FIG. 4 is a graph of UV absorption spectra of the color development of catalytically oxidized 3,3',5,5' -tetramethylbenzidine hydrochloride after the heparin-modified osmium nanoparticles are reacted with 50 μm final mercury ions under normal and nitrogen-loading conditions, respectively.
Fig. 5 is a graph of the color development effect of heparin-modified osmium nanoparticles and 50 μm mercury ion catalytic oxidation of 3,3',5,5' -tetramethylbenzidine hydrochloride versus heparin in the preparation of the heparin-modified osmium nanoparticles.
Fig. 6 is a graph showing the relationship between the color development effect of heparin modified osmium nanoparticles and 50 μm mercury ion catalytic oxidation 3,3',5,5' -tetramethylbenzidine hydrochloride and the pH of the reaction system.
Fig. 7 is a graph of the color development effect of heparin modified osmium nanoparticles and 50 μm mercury ions for catalytic oxidation of 3,3',5,5' -tetramethylbenzidine hydrochloride as a function of reaction temperature.
Fig. 8 is a graph of the color development effect versus reaction time of heparin modified osmium nanoparticles and 50 μm mercury ions for catalytic oxidation of 3,3',5,5' -tetramethylbenzidine hydrochloride.
Fig. 9 is a graph of the effect of different ions (1-24) on the color development system of heparin-modified osmium nanoparticles catalyzed oxidation of 3,3',5,5' -tetramethylbenzidine hydrochloride in an interference experiment for mercury ion detection. (1-24 respectively represent Mg 2+ 、Mn 2 + 、Ni 2+ 、Zn 2+ 、Ca 2+ 、Co 2+ 、Bi 3+ 、NH 4 + 、K + 、Ce 3+ 、Ba 2+ 、Na + 、Cu 2+ 、Pb 2+ 、Cr 3+ 、Fe 3+ 、Al 3+ 、Ag + 、F - 、Br 2- 、I - 、HCO 3 - 、CO 3 2- ，Hg 2+ Ion concentration 50 [ mu ] M
FIG. 10 is a graph of ultraviolet absorbance spectra of a color development system in the presence of different concentrations of mercury ions.
FIG. 11 is a standard graph of mercury ions and a color photograph comparison of a color development system in the presence of different concentrations of mercury ions, showing specific color changes: as the concentration of mercury ions increases, the color of the solution gradually darkens.
Detailed Description
The invention provides a rapid colorimetric method for detecting mercury ions, and relates to a colorimetric detection method for mercury ions based on osmium nanoparticle simulated oxidase. As shown in fig. 1, the detection method includes that the specific interaction of the heparin modified osmium nanoparticles and mercury ions is utilized to simulate the enhancement of oxidase activity, the heparin modified osmium nanoparticles after sensitization of the mercury ions are used for catalytic oxidation of 3,3',5,5' -tetramethyl biphenyl amine hydrochloride for color development, and as the content of the mercury ions is increased, the absorption value of a color development system at the maximum absorption wavelength of 600 nm is increased. Preliminary judgment according to the color of the color development system solution or quantification by a standard curve drawn according to the absorbance at wavelength 600 nm.
The technical scheme of the detection method of the invention is further described below with reference to the accompanying drawings and a plurality of embodiments.
Example 1:
heparin of 0.1 g is mixed with potassium hexachloroosmium with the concentration of 10 mM of 1 mL, 48 mL double distilled water is added, stirring is carried out for 30 minutes under the dark condition, then sodium borohydride with the concentration of 0.2M of 1 mL is added, stirring is continued for 90 minutes, the color of the solution is changed from light green to light brown, and 50 mL of heparin-modified osmium nano particles (with the concentration of 38 mg/L) are prepared. As shown in fig. 2, a is an osmium nanoparticle prepared without heparin ligand, and B is a heparin-modified osmium nanoparticle, and after 10 days of standing at room temperature, a is agglomerated and settled, while B heparin-modified osmium nanoparticle can maintain good stability.
Example 2:
to 0.4 mL of 50. Mu.M mercury ion sample solution, 0.1. 0.1 mL of the heparin-modified osmium nanoparticles prepared in example 1 (concentration: 38 mg/L) was added. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after reaction for 5 min in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. Fig. 3 is a schematic diagram of mercury ion detection, wherein the absorbance at the absorption wavelength 600 nm is significantly increased after mercury ion addition.
Example 3:
to 0.4 mL of 50. Mu.M mercury ion sample solution, 0.1. 0.1 mL of the heparin-modified osmium nanoparticles prepared in example 1 (concentration: 38 mg/L) was added. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after reaction for 5 min in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. As shown in fig. 3, the heparin-modified osmium nanoparticle without mercury ions catalyzes no significant change at the absorption wavelength 600 nm after 3,3',5,5' -tetramethylbenzidine hydrochloride, and the absorbance at the absorption wavelength 600 nm is significantly increased after mercury ions (50 μm) are added.
Example 4:
to 0.4 mL of 50. Mu.M mercury ion sample solution, 0.1. 0.1 mL of the heparin-modified osmium nanoparticles prepared in example 1 (concentration: 38 mg/L) was added. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after reaction for 5 min in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. As shown in fig. 4, the process is performed under the condition of nitrogen introduction and nitrogen non-introduction respectively, after nitrogen introduction, the heparin modified osmium nanoparticles added with mercury ions catalyze 3,3',5,5' -tetramethyl biphenyl amine hydrochloride, and no obvious change occurs at the absorption wavelength 600 nm, which indicates that the mercury ions sensitize the simulated oxidase activity of the heparin modified osmium nanoparticles and catalyze the reaction of oxygen and 3,3',5,5' -tetramethyl biphenyl amine hydrochloride.
Example 5:
0.1. 0.1 mL heparin-modified osmium nanoparticles (38 mg/L concentration) were added to 0.4 mL, 50. Mu.M mercury ion sample solution. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after reaction for 5 min in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. As shown in fig. 5, to achieve better mercury ion detection, the heparin concentration of the heparin-modified osmium nanoparticles was optimized, the optimized heparin concentration was 2 mg/mL, and the preparation method of the heparin-modified osmium nanoparticles was as in example 1.
Example 6:
0.1 mL example 1 heparin-modified osmium nanoparticles (38 mg/L concentration) were added to 0.4 mL, 50. Mu.M mercury ion sample solution. Then, 3300 mu L of phosphate buffer solution (different pH values, 20 mM) and 200 mu L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after the mixed solution is reacted for 5 min in a water bath at 25 ℃, the absorbance value at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. As shown in fig. 6, to achieve better mercury ion detection, the pH of the phosphate buffer solution was optimized, with an optimized pH of 6.
Example 7:
0.1 mL example 1 heparin-modified osmium nanoparticles (38 mg/L concentration) were added to 0.4 mL, 50. Mu.M mercury ion sample solution. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after the mixed solution reacts for 5 min in water baths with different temperatures, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. As shown in fig. 7, the reaction temperature during the above reaction process was optimized for better detection of mercury ions, and the optimized temperature was 25 ℃.
Example 8:
0.1 mL example 1 heparin-modified osmium nanoparticles (38 mg/L concentration) were added to 0.4 mL, 50. Mu.M mercury ion sample solution. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after different times of reaction in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. As shown in fig. 8, in order to achieve better detection of mercury ions, the reaction time of the above reaction process was optimized, and the reaction time after the optimization was 5 minutes.
Example 9:
0.1 mL example 1 heparin-modified osmium nanoparticles (38 mg/L concentration) were added to 0.4 mL different ion sample solutions. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after different times of reaction in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. As shown in fig. 9, to achieve better detection of mercury ions, the specificity of the system was verified, the response of the various ions to the system was compared, and compared to the enhancement signal generated by 50 μm mercury ions (24), 50 μm of other particles (1-23 are Mg respectively 2+ 、Mn 2+ 、Ni 2+ 、Zn 2+ 、Ca 2+ 、Co 2+ 、Bi 3+ 、NH 4 + 、K + 、Ce 3+ 、Ba 2+ 、Na + 、Cu 2+ 、Pb 2+ 、Cr 3+ 、Fe 3+ 、Al 3+ 、Ag + 、F - 、Br 2- 、I - 、HCO 3 - 、CO 3 2- ) Are negligible.
Example 10:
0.1 mL example 1 heparin-modified osmium nanoparticles (38 mg/L concentration) were added to 0.4 mL different concentrations (3-40 μm) of mercury ion sample solution. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after different times of reaction in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. The response spectra of the system for different concentrations (3-40 μm) of mercury ions as measured with an ultraviolet-visible spectrophotometer are shown in fig. 10.
Example 11:
0.1 mL example 1 heparin-modified osmium nanoparticles (38 mg/L concentration) were added to 0.4 mL different concentrations (3-40 μm) of mercury ion sample solution. Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after different times of reaction in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. As shown in fig. 11, when the mercury ions are not contained, the solution is colorless, and as the concentration of the mercury ions increases, the color of the system solution gradually changes to dark blue, and the detection limit of naked eyes is 3 μm. As the content of mercury ions increases (3-40 μm), the absorption value of the color development system at the maximum absorption wavelength 600 nm increases, and the color development system and the concentration of mercury ions are in a linear relation within the range of 3-40 μm, and the detection limit is 220 nM. Preliminary judgment according to the color of the color development system solution or quantification by a standard curve drawn according to the absorbance at wavelength 600 nm.
Example 12:
0.4 To a sample of mL tap water was added 0.1 mL example 1 heparin-modified osmium nanoparticles (38 mg/L). Then, 3300 [ mu ] L of phosphate buffer solution (pH=6, 20 mM) and 200 [ mu ] L of 3,3',5,5' -tetramethyl benzidine hydrochloride (2 mM) are added into the mixed solution, and after different times of reaction in a water bath at 25 ℃, the absorbance at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer. The content of mercury ions is calculated by combining the standard curve of the embodiment 11, the standard adding recovery rate of tap water samples is 96.47-103.85%, and the relative standard deviation is 0-2.04%, and the table 1 is shown.
TABLE 1 recovery of mercury ions from tap Water samples by labelling
Claims (5)
1. A mercury ion colorimetric detection method based on osmium nanoparticle simulated oxidase is characterized in that the activity of the simulated oxidase is enhanced after the specific interaction of heparin modified osmium nanoparticles and mercury ions, the heparin modified osmium nanoparticles after sensitization of mercury ions catalyze and oxidize 3,3',5,5' -tetramethyl benzidine hydrochloride to develop color, and the absorption value of a color development system at the position of 600 nm of the maximum absorption wavelength is increased along with the increase of the content of mercury ions; determining the concentration of mercury ions according to preliminary judgment of the color of the solution of the color development system or quantification by a standard curve drawn according to the absorbance at the wavelength of 600 nm;
the heparin-modified osmium nanoparticles used were prepared by the following method: mixing heparin of 0.1 g with potassium hexachloroosmium with the concentration of 1 mL and 10 mM, adding 48 mL double distilled water, stirring for 30 minutes under the dark condition, then adding sodium borohydride with the concentration of 1 mL and 0.2M, and continuously stirring for 90 minutes, wherein the color of the solution is changed from light green to light brown, and obtaining heparin modified osmium nanoparticles with the concentration of 50 mL and 38 mg/L;
adding 0.1 mL heparin-modified osmium nanoparticles with the concentration of 38 mg/L into 0.4 mL and 3-40 mu M mercury ion sample solutions with different concentrations; then 3300 MuL of phosphate buffer solution with pH value of 6, 20 MuL and mM MuL of 3,3',5,5' -tetramethyl benzidine hydrochloride with concentration of 2 mM are added into the mixed solution, after the mixed solution reacts for different time in a water bath with the temperature of 25 ℃, the absorbance value at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer; when the mercury ions are not contained, the solution is colorless, and the color of the system solution gradually changes into deep blue along with the increase of the concentration of the mercury ions, and the detection limit of naked eyes is 3 mu M; as the content of mercury ions increases, the absorption value of the color development system at the maximum absorption wavelength of 600 nm increases, the color development system and the concentration of mercury ions are in linear relation within the range of 3-40 mu M, and the detection limit is 220 nM; preliminary judgment according to the color of the color development system solution or quantification by a standard curve drawn according to the absorbance at wavelength 600 nm.
2. The method for colorimetric detection of mercury ions based on osmium nanoparticle simulated oxidase according to claim 1, wherein the concentration of mercury ions can be primarily judged according to the color of a solution of a color developing system.
3. The method for colorimetric detection of mercury ions by using osmium nanoparticle-based simulated oxidase according to claim 1, wherein the concentration of mercury ions can be determined based on the absorption value at the maximum absorption wavelength of 600 nm of the ultraviolet absorption spectrum.
4. The method for colorimetric detection of mercury ions based on osmium nanoparticle-based simulated oxidase according to claim 1, wherein the concentration of heparin is 2 mg/mL, the pH of the phosphate buffer solution is 6, the reaction temperature during the reaction is 25 ℃ and the reaction time is 5 minutes.
5. The colorimetric detection method of mercury ions based on osmium nanoparticle simulated oxidase is used for detecting the concentration of mercury ions in a water sample and is characterized by comprising the following steps of: taking a tap water sample of 0.4 and mL, and adding heparin-modified osmium nanoparticles with the concentration of 38.1 and mL being 38 mg/L; then 3300 MuL of phosphate buffer solution with pH value of 6, 20 MuL and mM MuL of 3,3',5,5' -tetramethyl benzidine hydrochloride with concentration of 2 mM are added into the mixed solution, after the mixed solution reacts for different time in a water bath with the temperature of 25 ℃, the absorbance value at the maximum absorption wavelength of 600 nm is measured by an ultraviolet-visible spectrophotometer; calculating the content of mercury ions by combining a standard curve to obtain the content of mercury ions in the water sample;
the heparin-modified osmium nanoparticles used were prepared by the following method: mixing heparin of 0.1 and g with potassium hexachloroosmium with the concentration of 1 mL and 10 mM, adding 48 mL double distilled water, stirring for 30 minutes under the dark condition, then adding sodium borohydride with the concentration of 1 mL and 0.2M, and continuously stirring for 90 minutes, wherein the color of the solution is changed from light green to light brown, and obtaining the heparin modified osmium nano-particles with the concentration of 50 mL and 38 mg/L.
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