CN112684076A - White peony root extract and construction method of fingerprint spectrum thereof - Google Patents
White peony root extract and construction method of fingerprint spectrum thereof Download PDFInfo
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Abstract
The invention particularly relates to a white paeony root extract and a construction method of a fingerprint spectrum thereof. The traditional cervicodynia granules are prepared by mixing radix paeoniae alba with other medicinal materials, adding water for extraction and carrying out water extraction and alcohol precipitation to obtain clear paste. The inventor considers that the extraction rate of the total paeoniflorin in the extraction process has large fluctuation under the influence of various factors such as temperature, crushing degree and the like. The invention provides a white peony root extract, which is prepared by firstly fully releasing active ingredients in medicinal materials through enzymolysis and then extracting total paeoniflorin through an alkaline alcohol solution. According to the detection result, the white paeony root extract prepared by the method contains key active ingredients in the white paeony root, and the solubility in water is improved.
Description
Technical Field
The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a white paeony root extract and a fingerprint construction method of the white paeony root extract.
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
Radix Paeoniae alba is dry root of Paeonia lactiflora pall of Ranunculaceae, and has bitter and sour taste, and liver and spleen meridians regulating, blood nourishing, menstruation regulating, yin astringing, and antiperspirant effects. It is used to treat headache, vertigo, abdominal pain, contracture and pain of limbs, blood deficiency, sallow complexion, menoxenia, spontaneous perspiration, and night sweat. The total glucosides of paeony are effective parts extracted from the traditional Chinese medicine white paeony root, have the functions of resisting inflammation, regulating immunity, protecting liver and the like, are mainly prepared into tablets, capsules and the like at present, and are commonly used for treating chronic hepatitis, rheumatic arthritis and the like. In previous researches, the inventor provides a medicinal preparation for treating cervical spondylosis, which comprises pseudo-ginseng, ligusticum wallichii, corydalis tuber, white paeony root, clematis chinensis, kudzu-vine root and notopterygium root. The research proves that the extraction effect of the total glucosides of paeony has important influence on the overall effect of the pharmaceutical preparation. According to the record of patent CN03112032.6, in the preparation method, the white paeony root and other medicinal materials are extracted into clear paste by adopting a water extraction and alcohol precipitation mode. The inventor finds that in the industrial production process, the white paeony root is used as a root medicinal material, has a hard phloem and has larger fluctuation of extraction efficiency when being mixed with other medicinal materials for extraction. The inventor considers that the white paeony root is firstly extracted to prepare a white paeony root extract, the obtained white paeony root total glycoside component can be directly used as a clinical medicine for application, and can also be directly applied to various pharmaceutical products related to the white paeony root, and the quality control of active ingredients in the medicine can be improved.
Aiming at more quality control research methods of radix paeoniae alba preparations in the prior art, the invention patent 'a radix paeoniae alba formula particle and a preparation method and a quality control method thereof' (patent publication No. CN101474263B) discloses a preparation and quality control method of the radix paeoniae alba formula particle, which uses a Fourier transform infrared spectrometer and a DTGS detector to carry out infrared fingerprint spectrum determination.
The invention patent of a quality detection method of a radix paeoniae alba preparation (CN102138985B) discloses a quality detection method of a radix paeoniae alba preparation, which uses acetonitrile-0.1% phosphoric acid aqueous solution (12:88) as a mobile phase, octadecylsilane chemically bonded silica as a filler chromatographic column to carry out content determination of paeoniflorin and albiflorin; the fingerprint in this patent identifies 6 characteristic peaks, but does not identify the components of the characteristic peaks.
The invention discloses a method for establishing a fingerprint of a radix paeoniae alba medicinal preparation (CN 105866296A). The fingerprint of the radix paeoniae alba medicinal preparation is established by selecting a chromatographic column with octadecylsilane chemically bonded silica as a filler and acetonitrile-0.1% phosphoric acid as a mobile phase (gradient elution). Determining 4 common characteristic peaks, and identifying three components: respectively hydroxy paeoniflorin, albiflorin and paeoniflorin. The fingerprint has fewer characteristic peaks, bad peak shape and trailing individual peaks.
The invention discloses a radix paeoniae alba fingerprint detection method (CN109490437A), which adopts a Merck C18 chromatographic column and acetonitrile-0.1% phosphoric acid aqueous solution as a mobile phase (gradient elution) to establish the radix paeoniae alba fingerprint detection method. Selecting common peaks in chromatograms of different batches of white paeony roots as characteristic peaks, determining the number of the characteristic peaks to be 8, and identifying the characteristic peaks: 1-8 are gallic acid, gallomethyl ester, oxypaeoniflorin, albiflorin, paeoniflorin, benzoic acid, 1,2,3,4, 6-pentagalloylglucose and benzoylpaeoniflorin respectively, but characteristic peaks 4, 5, 6 and 8 in a chromatogram of the method have trailing and poor peak shape.
Disclosure of Invention
Aiming at the research background, the invention aims to provide a white paeony root extract, which aims at reserving total glucosides of paeony in white paeony root, and further provides a fingerprint spectrum establishing method aiming at the white paeony root extract, so that a stable and reliable quality control method is provided for the application of the white paeony root extract in a medicinal preparation.
Based on the technical effects, the invention provides the following technical scheme:
in a first aspect of the present invention, a white peony root extract is provided, which is prepared by the following steps: adding enzyme water solution into the white peony material, and preserving heat for a period of time to obtain water extract; sequentially adding alkali and alcohol into the water extract until the precipitation is not increased any more, filtering and retaining the solution part, adding acid to adjust the pH of the solution to be neutral, and removing the solvent to obtain a medicinal extract, namely the white paeony root extract.
In the prior art, the extraction process of the total paeoniflorin in the peony is researched more, and in the prior art, the extraction method of the total paeoniflorin in the peony is commonly used in the field by adopting an alcohol solution, extraction and enzymolysis. In the extraction idea designed by the invention, active ingredients in the white paeony root medicinal material are fully dissolved out firstly through enzymolysis, the enzyme and the original protein in the medicinal material are denatured through an alkali adding mode, and then the alcohol solution is continuously added to change the characteristics of solution particles so as to approach the isoelectric point of the protein, so that the protein and polysaccharide impurities in the solution are fully precipitated. The radix paeoniae alba extract prepared based on the idea of the invention can fully reserve active ingredients such as total paeoniflorin and the like, and the conditions such as high-temperature heating and the like are not needed in the extraction process. Compared with the white paeony root extract prepared by the traditional water extraction and alcohol precipitation method, the white paeony root extract prepared by the method removes protein and fiber components in the traditional extract, effectively reduces the viscosity degree of the medicinal extract, improves the water solubility and is convenient for subsequent processing. In addition, the improvement of water solubility also means that higher bioavailability of the drug after entering the human body is expected. Furthermore, the invention also provides a method for constructing the fingerprint of the white paeony root extract in order to verify each active ingredient in the white paeony root extract.
In a second aspect of the present invention, the method for constructing a fingerprint of the white peony root extract of the first aspect is provided, and paeoniflorin, gallomethyl, gallic acid, benzoylpaeoniflorin, oxypaeoniflorin, 1,2,3,4, 6-pentagalloylglucose are used as mixed reference substances.
In the prior art, the white paeony root fingerprint spectrum construction method has the technical problems of poor peak shape, tailing, poor sample separation degree and the like in multi-sample analysis. The invention provides a fingerprint construction method aiming at a white peony root extract, which can determine 7 main active ingredients of paeony total glycosides, and each reference sample of the fingerprint has good separation effect and good stability, can be used as a quality control method, and has important significance for the application of the white peony root extract in pharmacy.
In a third aspect of the present invention, the quality control method of the white peony root extract of the first aspect is provided, and the quality control method includes detecting the content of paeoniflorin, gallomethyl, gallic acid, benzoylpaeoniflorin, oxypaeoniflorin and 1,2,3,4, 6-pentagalloylglucose in a sample to be detected by using the fingerprint spectrum construction method of the second aspect.
The beneficial effects of one or more technical schemes are as follows:
1. the white paeony root extract provided by the invention has a simple preparation process, and does not need high temperature and high pressure and addition of toxic organic reagents. The radix Paeoniae alba extract prepared by the method contains less plant protein and cellulose impurities, and can fully extract active ingredients in radix Paeoniae alba. In addition, compared with the traditional medicine extract, the white paeony root extract prepared by the method has better water solubility, which means that the white paeony root extract has better processing performance and bioavailability.
2. In order to realize the application of the white paeony root extract in the pharmaceutical field, the invention also provides a fingerprint construction method of the white paeony root extract, and the quality control of the white paeony root extract in the pharmaceutical field is realized. The method has the characteristics of good repeatability, obvious characteristics and the like, is more beneficial to embodying the integral specificity of the white paeony root medicinal material by applying a fingerprint spectrum technology and a traditional Chinese medicine similarity evaluation system, and is beneficial to comprehensively, accurately and objectively evaluating the characteristics of the white paeony root medicinal material.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
Figure 1 is a fingerprint of the white peony extract described in example 4.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
As introduced in the background art, in order to obtain a white paeony root extract of total glucosides of paeony, the invention provides the white paeony root extract and a fingerprint construction method of the white paeony root extract.
In a first aspect of the present invention, a white peony root extract is provided, which is prepared by the following steps: adding enzyme water solution into the white peony material, and preserving heat for a period of time to obtain water extract; sequentially adding alkali and alcohol into the water extract until the precipitation is not increased any more, filtering and retaining the solution part, adding acid to adjust the pH of the solution to be neutral, and removing the solvent to obtain a medicinal extract, namely the white paeony root extract.
Preferably, the radix paeoniae alba medicinal material is crushed to 30-50 meshes.
Preferably, the volume of the aqueous solution is 2-4 times of that of the white paeony root.
Preferably, the enzyme is one or a mixture of several of cellulase, amylase, acid protease and pectinase.
Preferably, the addition amount of the enzyme is 0.5-2.5% of the weight of the substrate.
Preferably, the enzyme activity is 30-40 u/mg.
In the embodiment with a better effect, the enzyme is cellulase or a complex enzyme of cellulase and pectinase, when the enzyme is used for enzymolysis, the pH value of the water extract is acidic, so that the enzymolysis efficiency can be effectively improved, and in addition, the research of the invention also finds that the enzymolysis efficiency can be effectively improved by keeping the temperature at 35-50 ℃ for 1-2 hours.
Preferably, alkali liquor is added into the water extract until the pH value of the water solution is 9-10.
Preferably, the water extract is added with alcohol, and the alcohol is preferably methanol or ethanol; in a more preferred embodiment, the above-mentioned
In some embodiments with better effects in the above technical solution, the alkali solution is NaOH, and the acid solution is hydrochloric acid.
In a second aspect of the present invention, the method for constructing a fingerprint of the white peony root extract of the first aspect is provided, and paeoniflorin, gallomethyl, gallic acid, benzoylpaeoniflorin, oxypaeoniflorin, 1,2,3,4, 6-pentagalloylglucose are used as mixed reference substances.
Preferably, the fingerprint construction method comprises the following steps: (1) preparing a test sample and a reference solution, wherein the test sample is the white paeony root extract of the first aspect; (2) and measuring the test article and the reference article, wherein the measuring process comprises the following steps: octadecylsilane chemically bonded silica chromatographic column is adopted, acetonitrile-trifluoroacetic acid is used as a mobile phase, and gradient elution is carried out for separation.
Further, in the step (1), the sample and the reference substance are prepared by using an alcoholic solution.
In a specific embodiment, the white peony root extract and the reference substance are prepared by using a 70% methanol solution.
Preferably, the mobile phase parameters are as follows: the mobile phase A is acetonitrile, and the mobile phase B is 0.04-0.06% trifluoroacetic acid.
Preferably, the gradient elution procedure is as follows: 0-10 min, 9% A → 11% A; 10-28 min, 11% A → 23% A; 28-43 min, 23% A → 50% A; 44-55 min, 90% A → 90%, 55-56 min, 90% A → 9% A; 56-66 min, 9% A → 9%.
Preferably, the detection wavelength is 260nm to 280 nm.
Preferably, the flow rate is 0.8-1.2 ml/min.
In a third aspect of the present invention, the quality control method of the white peony root extract of the first aspect is provided, and the quality control method includes detecting the content of paeoniflorin, gallomethyl, gallic acid, benzoylpaeoniflorin, oxypaeoniflorin, and 1,2,3,4, 6-pentagalloylglucose in a sample to be detected by using the fingerprint spectrum construction method of the second aspect.
In order to make the technical solutions of the present invention more clearly understood by those skilled in the art, the technical solutions of the present invention will be described in detail below with reference to specific embodiments.
Example 1
In the embodiment, the preparation method of the white paeony root extract is provided, the white paeony root medicinal material is crushed into 60 meshes, 2 times of volume of water is added, 30u/mg of cellulase and pectinase are added, and the pH value is 6.5; heating the reaction system in water bath to 40 deg.C, heating for 1 hr, and filtering to obtain water extract.
Slowly adding 1M NaOH into the water extract under magnetic stirring until pH reaches 9.0, and continuously adding ethanol until alcohol concentration reaches 70%, wherein the rotation speed is 100 r/min. And after all the ethanol is added, standing and storing the solution system at 4 ℃ for 24 hours until floccules at the bottom are not increased any more, filtering the solution system to obtain a supernatant, adding hydrochloric acid into the supernatant until the pH is neutral, filtering again, keeping the supernatant, concentrating under reduced pressure to obtain an extract, and completely drying the extract in a drying oven to obtain the white paeony root extract.
Example 2
In this embodiment, another method for preparing an extract of paeonia lactiflora is provided, which comprises the following steps: crushing the white paeony root medicinal material to 30 meshes, adding 3 times of water, adding cellulase till 35u/mg, and the pH value is 6.0; heating the reaction system in water bath to 50 deg.C, heating for 0.5h, and filtering to obtain water extract.
Adding 0.8M NaOH into the water extract under magnetic stirring until pH reaches 10.0, and further adding ethanol until alcohol concentration reaches 65%, wherein the rotation speed is 80 r/min. And after all the ethanol is added, standing and storing the solution system at 4 ℃ for 48 hours until floccules at the bottom are not increased any more, filtering the solution system to obtain a supernatant, adding hydrochloric acid into the supernatant until the pH is neutral, filtering again, keeping the supernatant, concentrating under reduced pressure to obtain an extract, and completely drying the extract in a drying oven to obtain the white paeony root extract.
Example 3
In this embodiment, a method for preparing a white peony root extract is provided, which includes the following steps: crushing the white paeony root medicinal material to 50 meshes, adding water with the volume being doubled, adding pectinase to 40u/mg, and controlling the pH value to 6.0; heating the reaction system in water bath to 35 deg.C, heating for 2 hr, and filtering to obtain water extract.
Adding 1.2M NaOH into the water extract under magnetic stirring until pH reaches 9.5, and further adding ethanol until alcohol concentration reaches 75%, wherein the rotation speed is 150 r/min. And after all the ethanol is added, standing and storing the solution system at 4 ℃ for 30h until floccules at the bottom are not increased any more, filtering the solution system to obtain a supernatant, adding hydrochloric acid into the supernatant until the pH is neutral, filtering again, keeping the supernatant, concentrating under reduced pressure to obtain an extract, and completely drying the extract in a drying oven to obtain the white paeony root extract.
In the present invention, the solubility of the white peony root extract described in the above examples 1-3 is also tested, and the white peony root extract is obtained by the method described in patent CN03112032.6 in the control group, and the preparation method is as follows: decocting radix Paeoniae alba with water twice (2 hr for the first time and 1 hr for the second time), mixing the filtrate with the above two water solutions, concentrating, precipitating with ethanol, recovering ethanol, and concentrating to obtain fluid extract (control group) with relative density of 1.25-1.30(50 deg.C). The invention detects the solubility of the drug extract in water and organic solvent in examples 1-3 and the control group, and the detection results are shown in the following table 1:
TABLE 1 determination of dissolution rate (%)
| Water (W) | Organic reagent (ethanol) | Content of paeoniflorin (%) | |
| Control group | 12％ | 69％ | 2.1 |
| Example 1 | 85％ | 97％ | 8.8 |
| Example 2 | 80％ | 93％ | 8.9 |
| Example 3 | 79％ | 91％ | 7.4 |
From the state of the extract, the white paeony root extract provided by the invention is in loose powder after being dried, and the medicinal extract prepared by the preparation method of the control group is in a tightly combined block shape. The white peony root extract prepared in example 1 dissolved faster when added into water, while the clear paste prepared in the control group dissolved slower in both water and organic solvent due to the sticky texture. From the dissolution rate, the solubility of the white paeony root extract prepared by the method of the invention in water is obviously improved compared with the traditional extract.
Example 4 fingerprint construction method of white peony root extract
In order to further determine whether the active site of the white paeony root can be fully reserved in the white paeony root extract or not, and provide a stable quality control method for the application of the white paeony root extract in pharmacy. In this embodiment, a corresponding fingerprint is constructed for the detection of the white peony root extract, and in this embodiment, a method for constructing a fingerprint of a white peony root extract is provided.
First, experimental material
1. Instrument, reagent and test sample
The instrument comprises the following steps: high performance liquid chromatograph: agilent 1260, G1311C infusion pump, G1329B autosampler system, G1315D diode array detector, Waters Empower3 workstation; METTLER TOLEDO XPE205 electronic balance KQ5200DA numerical control ultrasonic cleaner (Kunshan ultrasonic instruments Co., Ltd.); .
Reagent: methanol (chromatographically pure, Tianjin kang Kode pharmaceutical chemical Co., Ltd.), acetonitrile (chromatographically pure, Tianjin kang Kode pharmaceutical chemical Co., Ltd.), trifluoroacetic acid (analytically pure, Allantin reagent), and purified water (Wahaha mineral water).
15 different batches of white peony root material were purchased from three production sites, Anhui Bozhou, Zhejiang Ningbo and Zhejiang Pan, respectively, and white peony root extracts were prepared as described in example 1.
(1) Chromatographic conditions
Measuring by high performance liquid chromatography (high performance liquid chromatography 0512 in the four parts of the 2015 edition of Chinese pharmacopoeia). Waters Sun Fire C18(4.6 mm. times.250 mm, 5 μm) as a chromatographic column; acetonitrile-0.05 trifluoroacetic acid as a mobile phase, gradient elution was performed according to the following table, detection wavelength was 274nm, and flow rate was 1.0 mL/min.
Linear gradiometer
| Time (min) | Acetonitrile (%) | Trifluoroacetic acid (%) |
| 0 | 9 | 91 |
| 10 | 11 | 89 |
| 28 | 23 | 77 |
| 43 | 50 | 50 |
| 44 | 90 | 10 |
| 55 | 90 | 10 |
| 56 | 9 | 91 |
| 66 | 9 | 91 |
(2) Preparation of control solutions
Weighing penoniflorin, methyl gallate, gallic acid, benzoylpaeoniflorin, oxypaeoniflorin, and 1,2,3,4, 6-pentagalloylglucose reference substances respectively 8mg, precisely weighing; put into the same 25mL volumetric flask, dissolved and diluted to the mark by adding 70% methanol, and shaken up to be used as a reference solution.
(3) Preparation of test solution
Sieving radix Paeoniae alba medicinal material to obtain fine powder 3g, precisely weighing, placing in conical flask with plug, precisely adding 70% methanol solution 50mL, weighing, ultrasonic processing (power 250W, frequency 53kHz) for 60min, weighing again, supplementing the loss amount with 70% methanol solution, shaking, and filtering with 0.22 μm micrometer microporous membrane to obtain test solution.
(4) Fingerprint spectrum for determining white peony root extract by high performance liquid chromatography
Respectively injecting 15 batches of white paeony root extract test solution and mixed reference solution prepared by the methods in the steps (1) and (2) into a high performance liquid chromatograph under the chromatographic conditions that: column number Waters Sun Fire C18(4.6 mm. times.250 mm, 5 μm), mobile phase: phase A is acetonitrile, phase B is aqueous solution containing 0.05% trifluoroacetic acid by volume; gradient elution (0-10 min, 9-11% A, 10-28 min, 11-23% A, 28-43 min, 23-50% A, 44-55 min, 90% A, 56-66 min, 9% A); volume flow rate: 1.0mL/min-1, column temperature: sample size 10 μ L at 35 ℃, detection wavelength: 230nm) determining a fingerprint; determining the number of the characteristic peaks to be 7, and respectively using gallic acid, methyl gallate, oxypaeoniflorin, albiflorin, paeoniflorin, 1,2,3,4, 6-pentagalloylglucose and benzoylpaeoniflorin.
Deriving the fingerprints of the 15 batches of white paeony root extract test solution obtained in the step (4), selecting common peaks in the 15 batches of white paeony root extract test solutions by using a traditional Chinese medicine fingerprint similarity evaluation system, generating a control fingerprint of the white paeony root by using an average value calculation method, calculating the relative retention time and the relative peak area of each common peak, wherein the control fingerprint has 20 common fingerprint peaks (the fingerprint similarity of the 15 batches of white paeony root extract test solutions is more than 0.92).
Secondly, investigating the stability of the fingerprint
1. System suitability test and negative interference test
Under the chromatographic conditions, respectively measuring 20uL of reference solution, test solution and blank solvent, injecting into a liquid chromatograph, and recording chromatogram. The result shows that the separation degree between each component to be detected and the adjacent peak in the chromatogram of the test sample is more than 1.5, and the blank solvent is not interfered.
2. Precision test
Taking the same white paeony root extract, preparing a white paeony root extract test solution according to the method in the step (2) of the embodiment 4, repeatedly injecting samples under the chromatographic condition in the step (4) for 6 times, taking paeoniflorin as a reference peak, evaluating by using similarity evaluation software (2004A) of the national pharmacopoeia committee, wherein the similarity is 0.99, recording the peak area and the relative retention time of the common peak, and calculating the relative standard deviation, wherein the RSD of each component is less than 2%. The results show that the instrument precision is good.
3. Stability test
The sample solution was prepared according to the step (2) of example 1, and after the sample solution was prepared, 10ul of sample solution was precisely aspirated, injected into a liquid chromatograph, and the chromatographic peak area was recorded, and then measured every 2 hours, and 48 hours were examined, and the relative retention time of each common peak and the relative standard deviation of the relative peak area were calculated, and RSD was less than 2%, and the similarity was 0.99. The results show that the solution stability of the common components in the test sample is good within 48 hours.
4. Repeatability test
The product was taken, a test solution was prepared according to the step (2) of example 1, measurement was repeated 6 times, and the content of each component in the sample was calculated. The result shows that the relative retention time of all the common peaks and the relative peak area RSD are less than 2 percent, the similarity is 0.99, and the method has good repeatability.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The white paeony root extract is characterized by being prepared by the following steps: adding enzyme water solution into the white peony material, and preserving heat for a period of time to obtain water extract; sequentially adding alkali and alcohol into the water extract until the precipitation is not increased any more, filtering and retaining the solution part, adding acid to adjust the pH of the solution to be neutral, and removing the solvent to obtain a medicinal extract, namely the white paeony root extract.
2. The radix paeoniae alba extract of claim 1, wherein the radix paeoniae alba drug is ground to 30-50 mesh; or the volume of the aqueous solution is 2-4 times of that of the white paeony root.
3. The white peony extract according to claim 1, wherein the enzyme is selected from the group consisting of, but not limited to, cellulase, amylase, acid protease, pectinase;
or the addition amount of the enzyme is 0.5-2.5% of the weight of the substrate;
or the enzyme activity is 30-40 u/mg.
4. The radix Paeoniae alba extract of claim 3, wherein the enzyme is cellulase or a complex enzyme of cellulase and pectinase;
or, the pH of the water extract is acidic;
or adding an enzyme water solution and then preserving the heat for 1-2 hours at the temperature of 35-50 ℃.
5. The radix paeoniae alba extract of claim 1, wherein an alkali solution is added to the aqueous extract until the pH of the aqueous solution is 9-10;
preferably, the water extract is added with alcohol, and the alcohol is preferably methanol or ethanol; in a more preferable scheme, the alkali liquor is NaOH, and the acid liquor is hydrochloric acid.
6. The method for constructing fingerprint of the white peony root extract as claimed in any one of claims 1 to 5, wherein paeoniflorin, gallomethyl, gallic acid, benzoylpaeoniflorin, oxypaeoniflorin, 1,2,3,4, 6-pentagalloylglucose are used as mixed reference.
7. The method for constructing the fingerprint of the white paeony root extract as claimed in claim 6, which is characterized by comprising the following steps: (1) preparing a test sample and a reference solution, wherein the test sample is the white paeony root extract of any one of claims 1 to 5; (2) and measuring the test article and the reference article, wherein the measuring process comprises the following steps: octadecylsilane chemically bonded silica chromatographic column is adopted, acetonitrile-trifluoroacetic acid is used as a mobile phase, and gradient elution is carried out for separation.
8. The method for constructing fingerprints of the white paeony root extract as claimed in claim 7, wherein in the step (1), the test substance and the reference substance are prepared by using alcoholic solution; further, the white paeony root extract and the reference substance are prepared by adopting 70% methanol solution.
9. The method for constructing fingerprint spectrum of white peony root extract of claim 7, wherein the mobile phase parameters are as follows: the mobile phase A is acetonitrile, and the mobile phase B is 0.04-0.06% trifluoroacetic acid;
alternatively, the gradient elution procedure is as follows: 0-10 min, 9% A → 11% A; 10-28 min, 11% A → 23% A; 28-43 min, 23% A → 50% A; 44-55 min, 90% A → 90%, 55-56 min, 90% A → 9% A; 56-66 min, 9% A → 9%;
or, the detection wavelength is 260 nm-280 nm;
or, the flow rate is 0.8-1.2 ml/min.
10. The quality control method of the radix paeoniae alba extract as claimed in any one of claims 1 to 5, which is characterized in that the quality control method comprises the step of detecting the contents of paeoniflorin, gallomethyl ester, gallic acid, benzoylpaeoniflorin, oxypaeoniflorin and 1,2,3,4, 6-pentagalloylglucose in a sample to be detected by the fingerprint construction method as claimed in any one of claims 6 to 9.
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