CN112680462B - 一种人***瘤病毒35型/hpv35型l1/l2及其制备与应用 - Google Patents

Abstract

本发明提供一种人***瘤病毒35型/HPV35型L1/L2及其制备与应用，申请人对所述假病毒中的L1蛋白和L2蛋白的编码基因进行了优化，使得制备假病毒使用时稀释倍数明显提高，并且检测周期短，灵敏度高，可重复性好，结果判读简单，检测成本低，方法便于应用推广。

Description

一种人***瘤病毒35型/HPV35型L1/L2及其制备与应用

技术领域

本发明涉及生物技术领域，特别是涉及一种人***瘤病毒35型/HPV35型L1/L2及其制备与应用。

背景技术

***和***前病变是全世界妇女健康的一个主要问题。临床学、分子生物学、和流行病学调查已经证明人***瘤病毒(human papillomavirus，HPV)是***和宫颈非典型增生的主要病因。目前HPV已经发现200多种亚型，根据致病性不同分为高危型包括HPV16、 18、31、33、35、39、45等；低危型包括HPV6、11等。HPV为无包膜的环状双链DNA病毒，其衣壳是由72个主要衣壳蛋白Ll蛋白五聚体构成的二十面体结构，1个病毒粒子上含有360个Ll蛋白单体。世界卫生组织国际癌症研究署已明确的13种能够导致***和高度痛前宫颈上皮内病变的HR-HPV包括HPV16、HPV18、HPV31、HPV33、HPV35、HPV39、 HPV45、HPV51、HPV52、HPV56、HPV58、HPV59和HPV68。其中仅HPV68在IARC致痛物分级中属于2A级，其余12种均属于I类致癌物。

由于目前市售疫苗最多为9个亚型，因此越来越多的公司也在不断研发更多价型(如包括HPV 35)的HPV疫苗。根据世界卫生组织(WHO)对HPV疫苗质量、安全性及有效性指导原则，以及国家食品药品监督管理局(CFDA)的相关规定，疫苗成功的关键是诱导有效的体液免疫，产生具有保护作用的中和抗体。因此对抗体的中和活性测定是疫苗评估和质控的重要依据。研制有效的预防疫苗对于预防HPV的传播和相关疾病的发生有重要意义。 HPV疫苗研发的一个最终目标是使开发的疫苗诱导人体产生高滴度的中和抗体，所以中和抗体检测是HPV疫苗研发过程中一个必不可少、非常关键的环节是否能建立一个有效的，精确的，灵敏的，简便的中和抗体评价体系对HPV疫苗整个研发项目就显得十分重要。以构建假病毒为基础的中和试验是检测抗体活性最直接的方法。ZsGreen假病毒中和滴度检验法可以反映疫苗效力，是较好的方法，同时它还可以对天然HPV感染者及***患者血清中的特异性中和抗体进行检测，以确定其所感染的HPV型别及产生的中和抗体水平，为HPV预防性疫苗评价和***流行病学研究提供可靠的依据。现有的采用显微镜观察或流式细胞仪进行ZsGreen假病毒中和滴度检测的不足之处在于其显微镜法通过肉眼观察，灵敏度较低，流式细胞仪操作较复杂并且检验设备较为昂贵。而应用酶联荧光斑点分析仪对ZsGreen假病毒中和滴度检验时，对于荧光点数可以精确计数，相对于显微镜法的肉眼观察来说更为检验结果更为准确和灵敏。相对于流式细胞仪检验-块96孔板耗时约2～3小时，酶联荧光斑点分析仪-块96孔板耗时仅几分钟，检验耗时较短，一天可以进行上百块96孔板的计数，具有高通量等优点。

发明内容

鉴于以上所述现有技术的缺点，本发明的目的在于提供一种人***瘤病毒35型L1/L2蛋白的基因序列，用于解决现有技术中HPV 35型假病毒制备以及假病毒在HPV 35型样本中和抗体滴度应用的问题。

发明构思：申请人通过实验发现，通过将人***瘤病毒35型L1蛋白和L2蛋白的编码基因进行优化后制备获得的假病毒滴度和实验灵敏度更高。

本发明的一方面提供了一种人***瘤病毒35型L1蛋白和L2蛋白的编码基因，所述L1 蛋白编码基因的核苷酸序列如SEQ ID NO.4或SEQ ID NO.5所示；所述L2蛋白编码基因的核苷酸序列如SEQ ID NO.7或SEQ ID NO.8所示。

本发明的另一方面提供了一种重组表达载体，所述重组表达载体中含有上述编码基因。

本发明的另一方面提供了一种宿主细胞，所述宿主细胞中含有或者整合有上述重组表达载体。

进一步地，所述重组表达载体中还含有报告基因编码序列。

本发明的另一方面提供了人***瘤病毒35型L1蛋白和L2蛋白的编码基因、重组表达载体、或宿主细胞在制备人***瘤病毒35型假病毒中的用途。

本发明的另一方面提供了一种人***瘤病毒35型假病毒的构建方法，所述方法包括使用如核苷酸序列SEQ ID NO.4或SEQ ID NO.5所示的序列编码L1蛋白和核苷酸序列SEQID NO.7或SEQ ID NO.8所示的序列编码L2蛋白。

在知晓L1编码基因的情况下，本领域技术人员可根据现有知识构建人***瘤病毒35型假病毒，具体的操作方法包括以下步骤：

1)构建包含上述人***瘤病毒35型L1蛋白的编码基因的重组表达载体以及包含上述人***瘤病毒35型L2蛋白的编码基因的重组表达载体

2)将两种表达载体宿主细胞中培养并表达；

3)裂解细胞并收集目的蛋白。

进一步地，两种所述重组表达载体中还含有报告蛋白表达基因。

更进一步地，所述报告蛋白为荧光蛋白，如绿色荧光蛋白、红色荧光蛋白、黄色荧光蛋白、蓝色荧光蛋白的任意一种或几种。

如上所述，本发明的人***瘤病毒35型L1蛋白和L2蛋白的基因序列，具有以下有益效果：

本发明采用优化后的人***瘤病毒35L1蛋白和L2蛋白编码基因进行HPV 35型的假病毒制备，其假病毒使用稀释倍数明显提高，并且检测周期短，灵敏度高，可重复性好，结果判读简单，检测成本低，方法便于应用推广。

附图说明

图1基因序列优化前的HPV 35L1+HPV 35L2表达质粒制备HPV 35型假病毒荧光点数与假病毒稀释倍数的关系图

图2基因序列优化后的HPV 35L1-op1+HPV 35L2-op1表达质粒制备HPV 35型假病毒荧光点数与假病毒稀释倍数的关系图。

图3基因序列优化后的HPV 35L1-op2+HPV 35L2-op2表达质粒制备HPV 35型假病毒荧光点数与假病毒稀释倍数的关系图。

图4HPV 35型假病毒应用于测定血清中和抗体滴度荧光斑点计数图。

具体实施方式

以下通过特定的具体实例说明本发明的实施方式，本领域技术人员可由本说明书所揭露的内容轻易地了解本发明的其他优点与功效。本发明还可以通过另外不同的具体实施方式加以实施或应用，本说明书中的各项细节也可以基于不同观点与应用，在没有背离本发明的精神下进行各种修饰或改变。须知，下列实施例中未具体注明的工艺设备或装置均采用本领域内的常规设备或装置。此外应理解，本发明中提到的一个或多个方法步骤并不排斥在所述组合步骤前后还可以存在其他方法步骤或在这些明确提到的步骤之间还可以***其他方法步骤，除非另有说明；还应理解，本发明中提到的一个或多个设备/装置之间的组合连接关系并不排斥在所述组合设备/装置前后还可以存在其他设备/装置或在这些明确提到的两个设备/装置之间还可以***其他设备/装置，除非另有说明。而且，除非另有说明，各方法步骤的编号仅为鉴别各方法步骤的便利工具，而非为限制各方法步骤的排列次序或限定本发明可实施的范围，其相对关系的改变或调整，在无实质变更技术内容的情况下，当亦视为本发明可实施的范畴。

以上的实施例是为了说明本发明公开的实施方案，并不能理解为对本发明的限制。此外，本文所列出的各种修改以及发明中方法、组合物的变化，在不脱离本发明的范围和精神的前提下对本领域内的技术人员来说是显而易见的。虽然已结合本发明的多种具体优选实施例对本发明进行了具体的描述，但应当理解，本发明不应仅限于这些具体实施例。事实上，各种如上所述的对本领域内的技术人员来说显而易见的修改来获取发明都应包括在本发明的范围内。

实施例1 HPV 35L1蛋白和HPV 35L2蛋白编码基因的优化

基于密码子使用频率、RNA二级结构、GC含量等多个维度对HPV 35L1蛋白和HPV35L2 蛋白编码基因进行了优化。HPV 35L1蛋白氨基酸序列如SEQ ID NO.1所示(GenBank登录号：CAA52566.1)，HPV 35L2蛋白氨基酸序列如SEQ ID NO.2所示(GenBank登录号：CAA52565.1)。优化前HPV 35L1蛋白的编码基因为SEQ ID NO.3，优化后的HPV 35L1蛋白的编码基因为SEQ ID NO.4(HPV 35L1-op1)和SEQ ID NO.5(HPV 35L1-op2)。优化前 HPV35L2蛋白的编码基因为SEQ ID NO.6，优化后的HPV 35L2蛋白的编码基因为SEQ ID NO.7(HPV 35L2-op1)和SEQ ID NO.8(HPV 35L2-op2)。

SEQ ID NO.1(HPV 35L1蛋白氨基酸序列)

MSLWRSNEATVYLPPVSVSKVVSTDEYVTRTNIYYHAGSSRLLAVGHPYYAIKKQDSNKI AVPKVSGLQYRVFRVKLPDPNKFGFPDTSFYDPASQRLVWACTGVEVGRGQPLGVGISGH PLLNKLDDTENSNKYVGNSGTDNRECISMDYKQTQLCLIGCRPPIGEHWGKGTPCNANQV KAGECPPLELLNTVLQDGDMVDTGFGAMDFTTLQANKSDVPLDICSSICKYPDYLKMVSE PYGDMLFFYLRREQMFVRHLFNRAGTVGETVPADLYIKGTTGTLPSTSYFPTPSGSMVTSD AQIFNKPYWLQRAQGHNNGICWSNQLFVTVVDTTRSTNMSVCSAVSSSDSTYKNDNFKEY LRHGEEYDLQFIFQLCKITLTADVMTYIHSMNPSILEDWNFGLTPPPSGTLEDTYRYVTSQA VTCQKPSAPKPKDDPLKNYTFWEVDLKEKFSADLDQFPLGRKFLLQAGLKARPNFRLGKR AAPASTSKKSSTKRRKVKS

SEQ ID NO.2(HPV 35L2蛋白氨基酸序列)

MRHKRSTKRVKRASATQLYRTCKAAGTCPPDVIPKVEGNTVADQILKYGSMAVFFGGLGI GSGSGTGGRSGYVPLGTTPPTAATNIPIRPPVTVESIPLDTIGPLDSSIVSLVEETSFIESGAPV VTPRVPPTTGFTITTSTDTTPAILDVTSISTHDNPTFTDPSVLHPPTPAETSGHFVLSSSSISTH NYEEIPMDTFIVSTDSNNITNSTPIPGSRPTTRLGLYSKGTQQVKVVDPAFMTSPAKLITYDN PAYEGLNPDTTLQFEHEDISLAPDPDFMDIIALHRPALTSRKGTIRYSRVGNKRTMHTRSGK AIGARVHYYQDLSSITEDIELQPLQHVPSSLPHTTVSTSLNDGMFDIYAPIDTEEDIIFSASSN NTLYTTSNTAYVPSNTTIPLSSGYDIPITAGPDIVFNSNTITNTVLPVPTGPIYSIIADGGDFYL HPSYYLLKRRRKRIPYFFADVSVAV

SEQ ID NO.3(优化前HPV 35L1蛋白编码基因)

ATGTCTCTGTGGAGATCCAACGAGGCCACTGTCTACCTGCCTCCAGTTTCGGTGTCTAAG GTTGTGTCCACGGACGAGTACGTCACTAGAACCAACATCTACTACCACGCAGGTTCCTC TAGACTCCTGGCTGTTGGTCACCCTTACTATGCCATTAAGAAGCAGGACTCGAACAAGA TCGCCGTCCCAAAGGTTTCTGGCTTGCAGTACAGAGTGTTCAGAGTTAAGCTGCCAGAC CCTAACAAGTTCGGATTTCCAGACACCTCCTTCTACGACCCTGCTTCCCAGAGATTGGTT TGGGCATGCACTGGAGTCGAGGTGGGCAGAGGTCAGCCATTGGGAGTTGGTATCTCTGG CCACCCTTTGCTGAACAAGCTCGACGATACCGAGAACTCCAACAAGTACGTTGGCAACT CTGGAACCGACAACAGAGAGTGCATCTCGATGGACTACAAGCAGACCCAGTTGTGTCT CATCGGATGCAGACCACCTATTGGTGAACATTGGGGAAAGGGCACCCCTTGCAACGCCA ACCAGGTCAAGGCCGGAGAGTGTCCTCCATTGGAGCTTCTGAACACTGTTCTCCAAGATGGTGACATGGTTGACACCGGCTTTGGTGCTATGGACTTCACGACCTTGCAGGCCAACAA GTCCGACGTGCCACTTGACATCTGTTCTTCCATTTGCAAGTACCCTGATTACCTGAAGAT GGTTTCGGAGCCATACGGAGACATGCTCTTCTTTTACCTGAGAAGAGAGCAGATGTTCG TGAGACACTTGTTCAACAGAGCAGGAACTGTTGGTGAAACGGTCCCTGCTGACCTGTA CATCAAGGGCACCACTGGTACGTTGCCATCTACCTCGTACTTCCCTACTCCATCTGGTTC GATGGTCACCTCCGATGCCCAGATCTTCAACAAGCCATACTGGTTGCAGAGAGCCCAGG GACACAACAATGGCATTTGCTGGTCCAACCAGCTGTTCGTGACCGTCGTTGACACTACG AGATCCACCAACATGTCGGTGTGTTCTGCAGTCAGCTCTTCCGACTCGACCTACAAGAA CGACAACTTCAAGGAGTACCTCAGACACGGTGAAGAGTACGACCTGCAATTCATCTTCC AGTTGTGCAAGATCACCCTGACTGCTGACGTTATGACGTACATTCACTCCATGAACCCTT CGATCCTGGAGGACTGGAACTTCGGTCTTACTCCACCTCCATCTGGCACCTTGGAGGAC ACTTACAGATATGTCACCTCCCAAGCTGTTACGTGTCAGAAGCCTTCGGCCCCAAAGCCT AAGGACGATCCACTGAAGAACTACACCTTCTGGGAGGTTGACCTGAAGGAGAAGTTCT CCGCAGACCTCGACCAGTTCCCATTGGGCAGAAAGTTCCTGCTCCAAGCTGGATTGAAG GCCAGACCTAACTTCAGACTTGGCAAGAGAGCCGCTCCAGCATCTACCTCTAAGAAATCGTCCACGAAGCGCAGAAAGGTGAAGTCG

SEQ ID NO.4(优化后HPV 35L1蛋白编码基因HPV 35L1-op1)

ATGAGCCTGTGGAGAAGCAACGAGGCCACCGTGTACCTGCCCCCGGTGAGCGTGAGCA AGGTGGTGAGCACCGACGAGTACGTGACAAGAACCAACATCTACTACCACGCCGGCAG CAGCAGACTGCTGGCCGTGGGCCACCCCTACTACGCCATCAAGAAGCAAGACAGCAAC AAGATCGCCGTGCCCAAGGTGAGCGGCCTGCAGTACAGAGTGTTCAGAGTGAAGCTGC CCGACCCCAACAAGTTCGGCTTCCCCGACACTAGCTTCTATGACCCGGCTAGCCAAAGACTGGTGTGGGCGTGTACGGGCGTGGAGGTAGGTAGAGGGCAGCCACTGGGCGTGGGCA TCAGCGGCCACCCCCTGCTGAACAAGCTGGACGACACCGAGAACAGCAACAAGTACGT GGGCAACAGCGGCACCGACAACAGAGAGTGCATCAGCATGGACTACAAGCAGACACA GCTGTGCCTGATCGGCTGCAGACCCCCCATCGGCGAGCACTGGGGCAAAGGCACCCCG TGTAACGCTAACCAAGTCAAGGCGGGAGAGTGCCCCCCCCTGGAGCTGCTGAACACCG TGCTGCAAGACGGCGACATGGTGGACACCGGCTTCGGCGCCATGGACTTCACCACCCT GCAAGCCAACAAGAGCGACGTGCCCCTGGACATCTGCAGCAGCATCTGCAAGTACCCC GACTACCTGAAGATGGTGAGCGAGCCCTACGGCGACATGCTGTTCTTCTACCTGAGAAG AGAGCAGATGTTCGTGAGACACCTGTTCAACAGAGCCGGCACCGTGGGCGAGACCGTG CCCGCCGACCTGTACATCAAGGGCACCACCGGCACCCTGCCTAGCACAAGCTACTTCCC CACGCCTAGCGGCAGCATGGTGACAAGCGACGCTCAGATCTTCAACAAGCCCTACTGGC TGCAGAGAGCCCAAGGCCACAACAACGGCATCTGCTGGAGCAATCAGCTGTTCGTGAC CGTGGTGGACACCACAAGAAGCACCAACATGAGCGTGTGCAGCGCCGTGAGCAGCAG CGACAGCACCTACAAGAACGACAACTTCAAGGAGTACCTGAGACACGGCGAGGAGTAC GACCTGCAGTTCATCTTTCAGCTGTGCAAGATCACCCTGACCGCCGACGTGATGACCTA CATCCACAGCATGAACCCTAGCATCCTGGAGGACTGGAACTTCGGCCTGACCCCCCCTC CTAGCGGCACCCTGGAGGACACCTACAGATACGTGACAAGCCAAGCCGTGACCTGTCA GAAGCCTAGCGCCCCCAAGCCCAAGGACGACCCCCTGAAGAACTACACCTTCTGGGAGGTGGACCTGAAGGAGAAGTTCAGCGCCGACCTGGATCAGTTCCCCCTGGGCAGAAAGT TCCTGCTGCAAGCCGGCCTGAAGGCTAGACCCAACTTCAGACTGGGCAAGAGAGCCGC CCCCGCTAGCACAAGCAAGAAGAGCAGCACCAAGAGAAGAAAGGTGAAGAGC

SEQ ID NO.5(优化后HPV 35L1蛋白编码基因HPV 35L1-op2)

ATGAGCCTGTGGAGAAGCAATGAGGCCACAGTGTACCTGCCCCCTGTGTCTGTGAGCAA GGTGGTGAGCACAGATGAGTATGTGACAAGAACCAACATCTACTACCATGCTGGCAGCA GCAGACTGCTGGCTGTGGGCCACCCCTACTATGCCATCAAGAAGCAAGACAGCAACAA GATTGCTGTGCCCAAGGTGTCTGGCCTGCAGTACAGAGTGTTCAGAGTGAAGCTGCCTG ACCCCAACAAGTTTGGCTTCCCTGACACTAGCTTCTATGACCCTGCTAGCCAAAGACTG GTGTGGGCCTGTACTGGGGTGGAGGTAGGTAGAGGGCAGCCACTGGGGGTGGGCATCT CTGGCCACCCCCTGCTGAACAAGCTGGATGACACAGAGAACAGCAACAAGTATGTGGG CAACTCTGGCACAGACAACAGAGAGTGCATCAGCATGGACTACAAGCAGACACAGCTG TGCCTGATTGGCTGCAGACCCCCCATTGGGGAGCACTGGGGCAAAGGCACCCCCTGTAA TGCCAACCAAGTCAAGGCTGGAGAGTGCCCCCCCCTGGAGCTGCTGAACACAGTGCTG CAAGATGGGGACATGGTGGACACTGGCTTTGGGGCCATGGACTTCACCACCCTGCAAG CCAACAAGTCTGATGTGCCCCTGGACATCTGCAGCAGCATCTGCAAGTACCCTGACTAC CTGAAGATGGTGTCTGAGCCCTATGGGGACATGCTGTTCTTCTACCTGAGAAGAGAGCA GATGTTTGTGAGACACCTGTTCAACAGAGCTGGCACAGTGGGGGAGACAGTGCCTGCT GACCTGTACATCAAGGGCACCACTGGCACCCTGCCTAGCACAAGCTACTTCCCCACCCC CTCTGGCAGCATGGTGACCTCTGATGCTCAGATCTTCAACAAGCCCTACTGGCTGCAGA GAGCCCAAGGCCACAACAATGGCATCTGCTGGAGCAATCAGCTGTTTGTGACAGTGGT GGACACCACAAGAAGCACCAACATGTCTGTGTGCTCTGCTGTGAGCAGCTCTGACAGC ACCTACAAGAATGACAACTTCAAGGAGTACCTGAGACATGGGGAGGAGTATGACCTGC AGTTCATCTTTCAGCTGTGCAAGATCACCCTGACAGCTGATGTGATGACCTACATCCACA GCATGAACCCTAGCATCCTGGAGGACTGGAACTTTGGCCTGACCCCCCCTCCCTCTGGC ACCCTGGAGGACACCTACAGATATGTGACAAGCCAAGCTGTGACCTGTCAGAAGCCCTC TGCCCCCAAGCCCAAGGATGACCCCCTGAAGAACTACACCTTCTGGGAGGTGGACCTG AAGGAGAAGTTCTCTGCTGACCTGGATCAGTTCCCCCTGGGCAGAAAGTTCCTGCTGCA AGCTGGCCTGAAGGCTAGACCCAACTTCAGACTGGGCAAGAGAGCTGCCCCTGCTAGC ACAAGCAAGAAGAGCAGCACCAAGAGAAGAAAGGTGAAGAGC

SEQ ID NO.6(优化前HPV 35L2蛋白编码基因)

ATGCGCCACAAGCGGTCCACCAAGAGAGTGAAGAGGGCCTCTGCCACACAGCTGTACA GAACCTGCAAGGCAGCAGGAACATGTCCACCTGACGTGATCCCCAAGGTGGAGGGCA ACACCGTGGCCGATCAGATCCTGAAGTACGGCTCTATGGCCGTGTTCTTTGGCGGCCTG GGCATCGGATCTGGAAGCGGAACCGGCGGCCGGAGCGGCTATGTGCCACTGGGAACC ACACCACCAACAGCAGCAACCAATATCCCCATCCGGCCTCCAGTGACAGTGGAGTCCA TCCCCCTGGACACCATCGGCCCTCTGGATAGCTCCATCGTGTCTCTGGTGGAGGAGACA TCCTTCATCGAGTCTGGAGCACCTGTGGTGACCCCAAGGGTGCCACCTACCACAGGCTT TACAATCACCACATCCACCGACACCACACCTGCCATCCTGGATGTGACATCCATCTCTA CCCACGACAACCCAACCTTCACAGATCCATCTGTGCTGCACCCACCAACACCAGCAGA GACCAGCGGCCACTTTGTGCTGTCTAGCTCCTCTATCTCCACCCACAACTACGAGGAGA TCCCTATGGACACATTCATCGTGTCTACCGATAGCAACAATATCACAAATAGCACCCCT ATCCCAGGCTCCAGACCTACCACAAGGCTGGGCCTGTATAGCAAGGGCACCCAGCAGG TGAAGGTGGTGGACCCAGCCTTTATGACATCCCCCGCCAAGCTGATCACCTACGATAA CCCCGCCTATGAGGGCCTGAATCCAGACACCACACTGCAGTTCGAGCACGAGGATATCTCCCTGGCCCCCGACCCTGACTTCATGGACATCATCGCCCTGCACAGACCAGCCCTGAC AAGCAGGAAGGGCACCATCCGCTACTCCCGGGTGGGCAACAAGCGCACAATGCACAC CCGGAGCGGCAAGGCAATCGGAGCAAGGGTGCACTACTATCAGGACCTGAGCTCCATC ACCGAGGATATCGAGCTGCAGCCACTGCAGCACGTGCCCTCTAGCCTGCCTCACACCA CAGTGAGCACATCCCTGAATGACGGCATGTTCGATATCTACGCCCCCATCGACACCGA GGAGGATATCATCTTTTCCGCCTCCTCTAACAATACACTGTACACCACATCTAACACCG CCTATGTGCCTAGCAATACCACAATCCCACTGAGCTCCGGCTATGACATCCCTATCACA GCCGGCCCAGATATCGTGTTCAACAGCAATACCATCACAAATACCGTGCTGCCAGTGC CAACCGGACCAATCTACTCTATCATCGCAGACGGAGGCGACTTCTACCTGCACCCTAGCTACTATCTGCTGAAGCGGAGAAGGAAGCGGATCCCATATTTCTTTGCCGACGTGAGC GTGGCCGTG

SEQ ID NO.7(优化后HPV 35L2蛋白编码基因HPV 35L2-op1)

ATGAGACACAAGAGAAGCACCAAGAGAGTGAAGAGAGCTAGCGCCACACAGCTGTAC AGAACCTGCAAGGCCGCCGGCACCTGCCCCCCCGACGTGATCCCCAAGGTGGAGGGCA ACACCGTGGCCGATCAGATCCTGAAGTATGGCAGCATGGCTGTCTTCTTCGGCGGCCTG GGTATCGGCAGCGGAAGCGGCACCGGCGGCAGAAGTGGGTATGTGCCCCTTGGTACGA CACCCCCCACCGCCGCCACCAACATCCCCATCAGACCCCCCGTGACCGTGGAGAGCAT CCCCCTGGACACCATCGGCCCCCTGGACAGCAGCATCGTGAGCCTGGTGGAGGAGACT AGCTTCATCGAGAGCGGCGCCCCCGTGGTGACCCCTAGAGTGCCCCCCACCACCGGCT TCACCATCACCACAAGCACGGACACGACACCCGCCATCCTGGACGTGACCTCCATCAG CACTCACGATAACCCTACCTTTACCGACCCTAGCGTTCTGCATCCCCCCACGCCCGCCG AGACAAGCGGCCACTTCGTGCTGAGCAGCAGCAGCATCAGCACCCACAACTATGAGGA GATCCCCATGGACACCTTCATCGTGAGCACCGACAGCAACAACATCACAAACTCCACA CCCATTCCCGGCAGCAGACCCACCACAAGACTGGGCCTGTACAGCAAGGGCACACAGC AAGTGAAGGTGGTGGACCCCGCCTTCATGACAAGCCCCGCCAAGCTGATCACCTACGA CAACCCCGCCTACGAGGGCCTGAACCCCGACACAACGCTGCAGTTCGAGCACGAGGAC ATCAGCCTGGCCCCCGACCCCGACTTCATGGACATCATCGCCCTGCACAGACCCGCCCT GACAAGCAGAAAGGGCACCATCAGATACAGCAGAGTGGGCAACAAGAGAACCATGCA CACAAGAAGCGGCAAGGCCATCGGCGCTAGAGTGCACTACTACCAAGACCTGAGCAG CATCACCGAGGACATCGAGCTGCAGCCCCTGCAGCACGTTCCCTCGTCCCTGCCCCACA CCACCGTGAGCACAAGCCTGAACGACGGCATGTTCGACATCTACGCCCCCATCGACAC CGAGGAGGACATCATCTTCAGCGCTAGCAGCAACAACACCCTGTACACCACAAGCAAC ACCGCCTACGTGCCTAGCAACACCACCATCCCCCTGAGCAGCGGCTACGACATCCCCA TAACTGCCGGCCCCGACATCGTGTTCAACAGCAACACCATCACCAACACCGTGCTGCC CGTGCCCACCGGCCCCATCTACAGCATCATCGCCGACGGCGGCGACTTCTACCTGCACC CTAGCTACTACCTGCTGAAGAGAAGAAGAAAGAGAATCCCCTACTTCTTCGCCGACGT GAGCGTGGCCGTG

SEQ ID NO.8(优化后HPV 35L2蛋白编码基因HPV 35L2-op2)

ATGAGACACAAGAGAAGCACCAAGAGAGTGAAGAGAGCCTCTGCCACACAGCTGTAC AGAACCTGCAAGGCTGCTGGCACCTGCCCCCCTGATGTGATCCCCAAGGTGGAGGGCA ACACAGTGGCTGATCAGATCCTGAAGTATGGCAGCATGGCTGTCTTCTTTGGGGGCCTG GGTATTGGCTCTGGCTCTGGCACTGGGGGCAGAAGTGGGTATGTGCCCCTTGGTACCAC ACCCCCCACAGCTGCCACCAACATCCCCATCAGACCCCCTGTGACAGTGGAGAGCATCCCCCTGGACACCATTGGCCCCCTGGACAGCAGCATTGTGAGCCTGGTGGAGGAGACTAG CTTCATTGAGTCTGGGGCCCCTGTGGTGACCCCTAGAGTGCCCCCCACCACTGGCTTCA CCATCACCACAAGCACAGACACCACACCTGCCATCCTGGATGTGACCTCCATCAGCACT CATGACAACCCTACCTTTACAGACCCCTCTGTGCTGCATCCCCCCACCCCTGCTGAGACC TCTGGCCACTTTGTGCTGAGCAGCAGCAGCATCAGCACCCACAACTATGAGGAGATCCC CATGGACACCTTCATTGTGAGCACAGACAGCAACAACATCACAAACTCCACACCCATTC CTGGCAGCAGACCCACCACAAGACTGGGCCTGTACAGCAAGGGCACACAGCAAGTGA AGGTGGTGGACCCTGCCTTCATGACAAGCCCTGCCAAGCTGATCACCTATGACAACCCT GCCTATGAGGGCCTGAACCCTGACACAACCCTGCAGTTTGAGCATGAGGACATCAGCCT GGCCCCTGACCCTGACTTCATGGACATCATTGCCCTGCACAGACCTGCCCTGACAAGCA GAAAGGGCACCATCAGATACAGCAGAGTGGGCAACAAGAGAACCATGCACACAAGATC TGGCAAGGCCATTGGGGCTAGAGTGCACTACTACCAAGACCTGAGCAGCATCACAGAG GACATTGAGCTGCAGCCCCTGCAGCATGTGCCTAGCTCCCTGCCCCACACCACAGTGAG CACAAGCCTGAATGATGGCATGTTTGACATCTATGCCCCCATTGACACAGAGGAGGACATCATCTTCTCTGCTAGCAGCAACAACACCCTGTACACCACAAGCAACACAGCCTATGTG CCTAGCAACACCACCATCCCCCTGAGCTCTGGCTATGACATCCCCATAACTGCTGGCCCT GACATTGTGTTCAACAGCAACACCATCACCAACACAGTGCTGCCTGTGCCCACTGGCCC CATCTACAGCATCATTGCTGATGGGGGGGACTTCTACCTGCACCCTAGCTACTACCTGCT GAAGAGAAGAAGAAAGAGAATCCCCTACTTCTTTGCTGATGTGTCTGTGGCTGTG

实施例2 HPV 35型假病毒的构建及制备

步骤1：将HPV 35L1、HPV 35L1-op1、HPV 35L1-op2、HPV 35L2、HPV 35L2-op1、HPV35L2-op2、和EGFP(荧光蛋白报告)基因***pCDNA3.1质粒，分别得到表达质粒 35L1-pCDNA3.1(SEQ ID NO.9)、35L1-op1-pCDNA3.1(SEQ ID NO.10)、35L1-op2-pCDNA3.1 (SEQID NO.11)、35L2-pCDNA3.1(SEQ ID NO.12)、35L2-op1-pCDNA3.1(SEQ ID NO.13)、 35L2-op2-pCDNA3.1(SEQ ID NO.14)、EGFP-pCDNA3.1(SEQ ID NO.15)。

表达质粒35L1-pCDNA3.1序列(含优化前基因HPV 35L1)，SEQ ID NO.9：

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGTCTCTGTGGAGATCCAA CGAGGCCACTGTCTACCTGCCTCCAGTTTCGGTGTCTAAGGTTGTGTCCACGGACGAGT ACGTCACTAGAACCAACATCTACTACCACGCAGGTTCCTCTAGACTCCTGGCTGTTGGTC ACCCTTACTATGCCATTAAGAAGCAGGACTCGAACAAGATCGCCGTCCCAAAGGTTTCT GGCTTGCAGTACAGAGTGTTCAGAGTTAAGCTGCCAGACCCTAACAAGTTCGGATTTCC AGACACCTCCTTCTACGACCCTGCTTCCCAGAGATTGGTTTGGGCATGCACTGGAGTCG AGGTGGGCAGAGGTCAGCCATTGGGAGTTGGTATCTCTGGCCACCCTTTGCTGAACAAG CTCGACGATACCGAGAACTCCAACAAGTACGTTGGCAACTCTGGAACCGACAACAGAG AGTGCATCTCGATGGACTACAAGCAGACCCAGTTGTGTCTCATCGGATGCAGACCACCT ATTGGTGAACATTGGGGAAAGGGCACCCCTTGCAACGCCAACCAGGTCAAGGCCGGAGAGTGTCCTCCATTGGAGCTTCTGAACACTGTTCTCCAAGATGGTGACATGGTTGACACC GGCTTTGGTGCTATGGACTTCACGACCTTGCAGGCCAACAAGTCCGACGTGCCACTTGA CATCTGTTCTTCCATTTGCAAGTACCCTGATTACCTGAAGATGGTTTCGGAGCCATACGG AGACATGCTCTTCTTTTACCTGAGAAGAGAGCAGATGTTCGTGAGACACTTGTTCAACA GAGCAGGAACTGTTGGTGAAACGGTCCCTGCTGACCTGTACATCAAGGGCACCACTGG TACGTTGCCATCTACCTCGTACTTCCCTACTCCATCTGGTTCGATGGTCACCTCCGATGCC CAGATCTTCAACAAGCCATACTGGTTGCAGAGAGCCCAGGGACACAACAATGGCATTTG CTGGTCCAACCAGCTGTTCGTGACCGTCGTTGACACTACGAGATCCACCAACATGTCGG TGTGTTCTGCAGTCAGCTCTTCCGACTCGACCTACAAGAACGACAACTTCAAGGAGTAC CTCAGACACGGTGAAGAGTACGACCTGCAATTCATCTTCCAGTTGTGCAAGATCACCCT GACTGCTGACGTTATGACGTACATTCACTCCATGAACCCTTCGATCCTGGAGGACTGGA ACTTCGGTCTTACTCCACCTCCATCTGGCACCTTGGAGGACACTTACAGATATGTCACCT CCCAAGCTGTTACGTGTCAGAAGCCTTCGGCCCCAAAGCCTAAGGACGATCCACTGAA GAACTACACCTTCTGGGAGGTTGACCTGAAGGAGAAGTTCTCCGCAGACCTCGACCAG TTCCCATTGGGCAGAAAGTTCCTGCTCCAAGCTGGATTGAAGGCCAGACCTAACTTCAG ACTTGGCAAGAGAGCCGCTCCAGCATCTACCTCTAAGAAATCGTCCACGAAGCGCAGA AAGGTGAAGTCGTAATAGAAGCTTAAGTTTAAACCGCTGATCAGCCTCGACTGTGCCTT CTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTG CCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGA CAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCA GCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGT GTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTT CGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCG GGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGA TTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGAC GTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACC CTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAA AATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAG GGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAAT TAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAA GCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCC TAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGC AGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTG GAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATC AAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCT CCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTG CTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGA CCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCT GGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGG GACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCT GCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCT ACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGA AGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCC GAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCC ATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCG ACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGAT ATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGG ACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGA TTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCT GGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTA TTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCAT TTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGT ATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGA AATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC CTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTT CCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTC GTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGA ATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCA GGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGG ATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAG GTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTA GGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGT ATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTG ATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGC GCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAG TGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCAC CTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACT TGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTC GTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTAC CATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTAT CAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATC CGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAA TAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGG TATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTT GTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCG CAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGT AAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCG GCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAA CTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTA CCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCT TTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAA AGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTG AAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAAT AAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC

表达质粒35L1-op1-pCDNA3.1序列(含优化后基因HPV 35L1-op1)，SEQ ID NO.10：

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGAGCCTGTGGAGAAGCA ACGAGGCCACCGTGTACCTGCCCCCGGTGAGCGTGAGCAAGGTGGTGAGCACCGACGA GTACGTGACAAGAACCAACATCTACTACCACGCCGGCAGCAGCAGACTGCTGGCCGTG GGCCACCCCTACTACGCCATCAAGAAGCAAGACAGCAACAAGATCGCCGTGCCCAAGG TGAGCGGCCTGCAGTACAGAGTGTTCAGAGTGAAGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACTAGCTTCTATGACCCGGCTAGCCAAAGACTGGTGTGGGCGTGTACGG GCGTGGAGGTAGGTAGAGGGCAGCCACTGGGCGTGGGCATCAGCGGCCACCCCCTGCT GAACAAGCTGGACGACACCGAGAACAGCAACAAGTACGTGGGCAACAGCGGCACCGA CAACAGAGAGTGCATCAGCATGGACTACAAGCAGACACAGCTGTGCCTGATCGGCTGC AGACCCCCCATCGGCGAGCACTGGGGCAAAGGCACCCCGTGTAACGCTAACCAAGTCA AGGCGGGAGAGTGCCCCCCCCTGGAGCTGCTGAACACCGTGCTGCAAGACGGCGACAT GGTGGACACCGGCTTCGGCGCCATGGACTTCACCACCCTGCAAGCCAACAAGAGCGAC GTGCCCCTGGACATCTGCAGCAGCATCTGCAAGTACCCCGACTACCTGAAGATGGTGAG CGAGCCCTACGGCGACATGCTGTTCTTCTACCTGAGAAGAGAGCAGATGTTCGTGAGACACCTGTTCAACAGAGCCGGCACCGTGGGCGAGACCGTGCCCGCCGACCTGTACATCAA GGGCACCACCGGCACCCTGCCTAGCACAAGCTACTTCCCCACGCCTAGCGGCAGCATGG TGACAAGCGACGCTCAGATCTTCAACAAGCCCTACTGGCTGCAGAGAGCCCAAGGCCA CAACAACGGCATCTGCTGGAGCAATCAGCTGTTCGTGACCGTGGTGGACACCACAAGA AGCACCAACATGAGCGTGTGCAGCGCCGTGAGCAGCAGCGACAGCACCTACAAGAACG ACAACTTCAAGGAGTACCTGAGACACGGCGAGGAGTACGACCTGCAGTTCATCTTTCA GCTGTGCAAGATCACCCTGACCGCCGACGTGATGACCTACATCCACAGCATGAACCCTA GCATCCTGGAGGACTGGAACTTCGGCCTGACCCCCCCTCCTAGCGGCACCCTGGAGGAC ACCTACAGATACGTGACAAGCCAAGCCGTGACCTGTCAGAAGCCTAGCGCCCCCAAGCCCAAGGACGACCCCCTGAAGAACTACACCTTCTGGGAGGTGGACCTGAAGGAGAAGTT CAGCGCCGACCTGGATCAGTTCCCCCTGGGCAGAAAGTTCCTGCTGCAAGCCGGCCTG AAGGCTAGACCCAACTTCAGACTGGGCAAGAGAGCCGCCCCCGCTAGCACAAGCAAGA AGAGCAGCACCAAGAGAAGAAAGGTGAAGAGCTAATAGAAGCTTAAGTTTAAACCGCT GATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGC CTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTG CATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGC AAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG CTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGC GGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAG CGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTT CCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCAC CTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATA GACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCA AACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCG ATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCT GTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGT ATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCC AGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCC TAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCT GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGA AGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGT ATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAA GATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTG GGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGG CGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGA GGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACG TTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCT CCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCG GCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCG AGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGA GCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGAC GGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAAT GGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGA CATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCT TCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCT TGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCA ACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAA TCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTC TTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCA CAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCAT CAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGG TCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCC GGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGC GTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAAT CGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCA CTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCG GTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCT CCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCG ACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTT CCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTT TCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGC TGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTT GAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGAT TAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG GCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTC TACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT TATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTA AAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTAT CTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACG CTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGA AGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGA GTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTG GTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGA GTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT GTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCT CTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCA TTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAAT ACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCG AAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCAC CCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGA AGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATAC TCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATA TTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC

表达质粒35L1-op2-pCDNA3.1序列(含优化后基因HPV 35L1-op2)，SEQ ID NO.11：

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGAGCCTGTGGAGAAGCA ATGAGGCCACAGTGTACCTGCCCCCTGTGTCTGTGAGCAAGGTGGTGAGCACAGATGA GTATGTGACAAGAACCAACATCTACTACCATGCTGGCAGCAGCAGACTGCTGGCTGTGG GCCACCCCTACTATGCCATCAAGAAGCAAGACAGCAACAAGATTGCTGTGCCCAAGGTG TCTGGCCTGCAGTACAGAGTGTTCAGAGTGAAGCTGCCTGACCCCAACAAGTTTGGCTT CCCTGACACTAGCTTCTATGACCCTGCTAGCCAAAGACTGGTGTGGGCCTGTACTGGGG TGGAGGTAGGTAGAGGGCAGCCACTGGGGGTGGGCATCTCTGGCCACCCCCTGCTGAA CAAGCTGGATGACACAGAGAACAGCAACAAGTATGTGGGCAACTCTGGCACAGACAAC AGAGAGTGCATCAGCATGGACTACAAGCAGACACAGCTGTGCCTGATTGGCTGCAGAC CCCCCATTGGGGAGCACTGGGGCAAAGGCACCCCCTGTAATGCCAACCAAGTCAAGGC TGGAGAGTGCCCCCCCCTGGAGCTGCTGAACACAGTGCTGCAAGATGGGGACATGGTG GACACTGGCTTTGGGGCCATGGACTTCACCACCCTGCAAGCCAACAAGTCTGATGTGCC CCTGGACATCTGCAGCAGCATCTGCAAGTACCCTGACTACCTGAAGATGGTGTCTGAGC CCTATGGGGACATGCTGTTCTTCTACCTGAGAAGAGAGCAGATGTTTGTGAGACACCTG TTCAACAGAGCTGGCACAGTGGGGGAGACAGTGCCTGCTGACCTGTACATCAAGGGCA CCACTGGCACCCTGCCTAGCACAAGCTACTTCCCCACCCCCTCTGGCAGCATGGTGACC TCTGATGCTCAGATCTTCAACAAGCCCTACTGGCTGCAGAGAGCCCAAGGCCACAACAA TGGCATCTGCTGGAGCAATCAGCTGTTTGTGACAGTGGTGGACACCACAAGAAGCACC AACATGTCTGTGTGCTCTGCTGTGAGCAGCTCTGACAGCACCTACAAGAATGACAACTTCAAGGAGTACCTGAGACATGGGGAGGAGTATGACCTGCAGTTCATCTTTCAGCTGTGCA AGATCACCCTGACAGCTGATGTGATGACCTACATCCACAGCATGAACCCTAGCATCCTGG AGGACTGGAACTTTGGCCTGACCCCCCCTCCCTCTGGCACCCTGGAGGACACCTACAGA TATGTGACAAGCCAAGCTGTGACCTGTCAGAAGCCCTCTGCCCCCAAGCCCAAGGATGA CCCCCTGAAGAACTACACCTTCTGGGAGGTGGACCTGAAGGAGAAGTTCTCTGCTGAC CTGGATCAGTTCCCCCTGGGCAGAAAGTTCCTGCTGCAAGCTGGCCTGAAGGCTAGACC CAACTTCAGACTGGGCAAGAGAGCTGCCCCTGCTAGCACAAGCAAGAAGAGCAGCACC AAGAGAAGAAAGGTGAAGAGCTAATAGAAGCTTAAGTTTAAACCGCTGATCAGCCTCG ACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACC CTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGT CTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGG ATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCG GAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAG CGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGC CCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAG CTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCA AAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTC GCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACA ACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTA TTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTG TGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCA TGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAG AAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCC CATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTT TTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGA GGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTC GGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCA CGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGA CAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGC TATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAA GCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCA CCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCT TGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTA CTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTC GCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCG TCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTG GATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCT ACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTA CGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTT CTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCAC GAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGG ACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCC AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAA ATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAT CATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTC CTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGT GTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGC CCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG GGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCG CTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGAC GAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAA CCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGA AGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGG TAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGC AGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCT GACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAG GATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATG AGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCT GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTC CAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGC AACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTC GCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTC GTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATC CCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTA AGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT GCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATA GTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACA TAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAA GGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTT CAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCC GCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCA ATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTT AGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC

表达质粒35L2-pCDNA3.1序列(含优化前基因HPV 35L2)，SEQ ID NO.12：

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGCGCCACAAGCGGTCCA CCAAGAGAGTGAAGAGGGCCTCTGCCACACAGCTGTACAGAACCTGCAAGGCAGCAG GAACATGTCCACCTGACGTGATCCCCAAGGTGGAGGGCAACACCGTGGCCGATCAGAT CCTGAAGTACGGCTCTATGGCCGTGTTCTTTGGCGGCCTGGGCATCGGATCTGGAAGCG GAACCGGCGGCCGGAGCGGCTATGTGCCACTGGGAACCACACCACCAACAGCAGCAACCAATATCCCCATCCGGCCTCCAGTGACAGTGGAGTCCATCCCCCTGGACACCATCGGCCC TCTGGATAGCTCCATCGTGTCTCTGGTGGAGGAGACATCCTTCATCGAGTCTGGAGCACC TGTGGTGACCCCAAGGGTGCCACCTACCACAGGCTTTACAATCACCACATCCACCGACA CCACACCTGCCATCCTGGATGTGACATCCATCTCTACCCACGACAACCCAACCTTCACAG ATCCATCTGTGCTGCACCCACCAACACCAGCAGAGACCAGCGGCCACTTTGTGCTGTCT AGCTCCTCTATCTCCACCCACAACTACGAGGAGATCCCTATGGACACATTCATCGTGTCT ACCGATAGCAACAATATCACAAATAGCACCCCTATCCCAGGCTCCAGACCTACCACAAG GCTGGGCCTGTATAGCAAGGGCACCCAGCAGGTGAAGGTGGTGGACCCAGCCTTTATGA CATCCCCCGCCAAGCTGATCACCTACGATAACCCCGCCTATGAGGGCCTGAATCCAGAC ACCACACTGCAGTTCGAGCACGAGGATATCTCCCTGGCCCCCGACCCTGACTTCATGGA CATCATCGCCCTGCACAGACCAGCCCTGACAAGCAGGAAGGGCACCATCCGCTACTCCC GGGTGGGCAACAAGCGCACAATGCACACCCGGAGCGGCAAGGCAATCGGAGCAAGGG TGCACTACTATCAGGACCTGAGCTCCATCACCGAGGATATCGAGCTGCAGCCACTGCAG CACGTGCCCTCTAGCCTGCCTCACACCACAGTGAGCACATCCCTGAATGACGGCATGTT CGATATCTACGCCCCCATCGACACCGAGGAGGATATCATCTTTTCCGCCTCCTCTAACAAT ACACTGTACACCACATCTAACACCGCCTATGTGCCTAGCAATACCACAATCCCACTGAGC TCCGGCTATGACATCCCTATCACAGCCGGCCCAGATATCGTGTTCAACAGCAATACCATC ACAAATACCGTGCTGCCAGTGCCAACCGGACCAATCTACTCTATCATCGCAGACGGAGG CGACTTCTACCTGCACCCTAGCTACTATCTGCTGAAGCGGAGAAGGAAGCGGATCCCATA TTTCTTTGCCGACGTGAGCGTGGCCGTGTAATAGAAGCTTAAGTTTAAACCGCTGATCAG CCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTT GACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCA TTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGG GAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGA GGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCA TTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCT AGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGA CCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGG TTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTG GAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTC GGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGG AATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGC AAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACT CCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTA ATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAG TGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATC CATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATG GATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCA CAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCC GGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAG CGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTC ACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGT CATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGC ATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGA GCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCA GGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAG GATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGC TTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGC GTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCG TGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGA GTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGC CATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTT TCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCC CACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATT TCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGT ATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAG CTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGC ATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGC TCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCA ACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACT CGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATA CGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCC CCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGA GCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACAC TAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAG CAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG GTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA AAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTAT ATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGC GATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATA CGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACC GGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGT CCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGT AGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCA CGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACA TGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGA AGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTG TCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAG AATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGC CACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTC TCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGA TCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAA TGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTT TTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGT ATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGA CGTC

表达质粒35L2-op1-pCDNA3.1序列(含优化后基因HPV 35L2-op1)，SEQ ID NO.13：

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGAGACACAAGAGAAGCA CCAAGAGAGTGAAGAGAGCTAGCGCCACACAGCTGTACAGAACCTGCAAGGCCGCCG GCACCTGCCCCCCCGACGTGATCCCCAAGGTGGAGGGCAACACCGTGGCCGATCAGAT CCTGAAGTATGGCAGCATGGCTGTCTTCTTCGGCGGCCTGGGTATCGGCAGCGGAAGCG GCACCGGCGGCAGAAGTGGGTATGTGCCCCTTGGTACGACACCCCCCACCGCCGCCACCAACATCCCCATCAGACCCCCCGTGACCGTGGAGAGCATCCCCCTGGACACCATCGGCC CCCTGGACAGCAGCATCGTGAGCCTGGTGGAGGAGACTAGCTTCATCGAGAGCGGCGC CCCCGTGGTGACCCCTAGAGTGCCCCCCACCACCGGCTTCACCATCACCACAAGCACGG ACACGACACCCGCCATCCTGGACGTGACCTCCATCAGCACTCACGATAACCCTACCTTTA CCGACCCTAGCGTTCTGCATCCCCCCACGCCCGCCGAGACAAGCGGCCACTTCGTGCTG AGCAGCAGCAGCATCAGCACCCACAACTATGAGGAGATCCCCATGGACACCTTCATCGT GAGCACCGACAGCAACAACATCACAAACTCCACACCCATTCCCGGCAGCAGACCCACC ACAAGACTGGGCCTGTACAGCAAGGGCACACAGCAAGTGAAGGTGGTGGACCCCGCCT TCATGACAAGCCCCGCCAAGCTGATCACCTACGACAACCCCGCCTACGAGGGCCTGAAC CCCGACACAACGCTGCAGTTCGAGCACGAGGACATCAGCCTGGCCCCCGACCCCGACT TCATGGACATCATCGCCCTGCACAGACCCGCCCTGACAAGCAGAAAGGGCACCATCAGA TACAGCAGAGTGGGCAACAAGAGAACCATGCACACAAGAAGCGGCAAGGCCATCGGC GCTAGAGTGCACTACTACCAAGACCTGAGCAGCATCACCGAGGACATCGAGCTGCAGC CCCTGCAGCACGTTCCCTCGTCCCTGCCCCACACCACCGTGAGCACAAGCCTGAACGA CGGCATGTTCGACATCTACGCCCCCATCGACACCGAGGAGGACATCATCTTCAGCGCTA GCAGCAACAACACCCTGTACACCACAAGCAACACCGCCTACGTGCCTAGCAACACCAC CATCCCCCTGAGCAGCGGCTACGACATCCCCATAACTGCCGGCCCCGACATCGTGTTCA ACAGCAACACCATCACCAACACCGTGCTGCCCGTGCCCACCGGCCCCATCTACAGCATC ATCGCCGACGGCGGCGACTTCTACCTGCACCCTAGCTACTACCTGCTGAAGAGAAGAAG AAAGAGAATCCCCTACTTCTTCGCCGACGTGAGCGTGGCCGTGTAATAGAAGCTTAAGT TTAAACCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCC TCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAAT GAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGG GCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTG GGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGC GCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTA CACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGT TCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTG CTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCA TCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGA CTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAG GGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACG CGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAG CAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCC CCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAT AGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCC GCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGA GCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCA TGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTC GGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTC AGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAAC TGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGC TGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCG GGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGAT GCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAA ACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATC TGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCG CATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCAT GGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACC GCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGG GCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTC TATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAA GCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTT GGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCA TGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAA GCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTT GTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTG GCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA ACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTC ACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT GCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCG CTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCT CACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACAT GTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTT TTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTG CGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGA AGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGC TCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGG TAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGG TGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCA GTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAG CGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATC CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT TTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGT TTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCA GTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGT CGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACC GCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGG GCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGC CGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCT ACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAA CGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGG TCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGC ACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTAC TCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTC AATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAAC GTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAA CCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGA GCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGT TGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCAT GAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATT TCCCCGAAAAGTGCCACCTGACGTC

表达质粒35L2-op2-pCDNA3.1序列(含优化后基因HPV 35L2-op2)，SEQ ID NO.14：

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGAGACACAAGAGAAGCA CCAAGAGAGTGAAGAGAGCCTCTGCCACACAGCTGTACAGAACCTGCAAGGCTGCTGG CACCTGCCCCCCTGATGTGATCCCCAAGGTGGAGGGCAACACAGTGGCTGATCAGATCC TGAAGTATGGCAGCATGGCTGTCTTCTTTGGGGGCCTGGGTATTGGCTCTGGCTCTGGCA CTGGGGGCAGAAGTGGGTATGTGCCCCTTGGTACCACACCCCCCACAGCTGCCACCAAC ATCCCCATCAGACCCCCTGTGACAGTGGAGAGCATCCCCCTGGACACCATTGGCCCCCT GGACAGCAGCATTGTGAGCCTGGTGGAGGAGACTAGCTTCATTGAGTCTGGGGCCCCTG TGGTGACCCCTAGAGTGCCCCCCACCACTGGCTTCACCATCACCACAAGCACAGACACC ACACCTGCCATCCTGGATGTGACCTCCATCAGCACTCATGACAACCCTACCTTTACAGAC CCCTCTGTGCTGCATCCCCCCACCCCTGCTGAGACCTCTGGCCACTTTGTGCTGAGCAGCAGCAGCATCAGCACCCACAACTATGAGGAGATCCCCATGGACACCTTCATTGTGAGCA CAGACAGCAACAACATCACAAACTCCACACCCATTCCTGGCAGCAGACCCACCACAAG ACTGGGCCTGTACAGCAAGGGCACACAGCAAGTGAAGGTGGTGGACCCTGCCTTCATG ACAAGCCCTGCCAAGCTGATCACCTATGACAACCCTGCCTATGAGGGCCTGAACCCTGA CACAACCCTGCAGTTTGAGCATGAGGACATCAGCCTGGCCCCTGACCCTGACTTCATGG ACATCATTGCCCTGCACAGACCTGCCCTGACAAGCAGAAAGGGCACCATCAGATACAGC AGAGTGGGCAACAAGAGAACCATGCACACAAGATCTGGCAAGGCCATTGGGGCTAGAG TGCACTACTACCAAGACCTGAGCAGCATCACAGAGGACATTGAGCTGCAGCCCCTGCA GCATGTGCCTAGCTCCCTGCCCCACACCACAGTGAGCACAAGCCTGAATGATGGCATGT TTGACATCTATGCCCCCATTGACACAGAGGAGGACATCATCTTCTCTGCTAGCAGCAACA ACACCCTGTACACCACAAGCAACACAGCCTATGTGCCTAGCAACACCACCATCCCCCTG AGCTCTGGCTATGACATCCCCATAACTGCTGGCCCTGACATTGTGTTCAACAGCAACACC ATCACCAACACAGTGCTGCCTGTGCCCACTGGCCCCATCTACAGCATCATTGCTGATGGG GGGGACTTCTACCTGCACCCTAGCTACTACCTGCTGAAGAGAAGAAGAAAGAGAATCCCCTACTTCTTTGCTGATGTGTCTGTGGCTGTGTAATAGAAGCTTAAGTTTAAACCGCTGAT CAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTT CCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCAT CGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAG GGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGC GCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGC CCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCC CGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTC GACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGAC GGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAAC TGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATT TCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGT GGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTAT GCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAG CAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTA ACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGA CTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGT AGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATA TCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGAT GGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGC ACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGC CCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGC AGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTG TCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCT GTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCT GCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGC GAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCAT CAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCG AGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCC GCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATA GCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCT CGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGAC GAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGT TTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCG CCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAA ATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAA TGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCAT AGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAA GCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGC GCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC CAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGA CTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCA GCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGC CCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGC AGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGC AAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTAC GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT CAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTC AGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACG ATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTC ACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGT GGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTA AGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTG TCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTT ACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTC AGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTT ACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTC TGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACC GCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAA ACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAA CTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGC AAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTC CTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGA ATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCAC CTGACGTC

表达质粒EGFP-pCDNA3.1序列(含荧光蛋白基因EGFP)，SEQ ID NO.15：

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGGTGAGCAAGGGCGAGG AGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCA CAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTG AAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCT GACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGAC GGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCA TCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGA GTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCA AGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCA CTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACC TGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCT GCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAT AGAAGCTTAAGTTTAAACCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCT GTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTT TCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGG GGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCT GGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGG GGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCC TTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGG TTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCA CGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTC TTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAAC AAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCC CAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGG TGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTA GTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTC CGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCC TCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGC AAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGG ATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGG AGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTG TTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGC CCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTT CCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGG CGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCA TCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACC ACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGAT CAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGC TCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTG CCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGT GTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGG CGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCG CATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATG ACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTA TGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCG GGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTA CAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAG TTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCT AGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAT TCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGA GCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGT GCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC TCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTA TCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAA GAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCC TTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGT CGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTG AAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTG AAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG CTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAA GAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTA AGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAA ATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGC TTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGAC TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAA TGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCC GGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAAT TGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGC CATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGG TTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCT CCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTA TGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGG TGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCC GGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGG AAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGA TGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTG GGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGA AATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGT CTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGC ACATTTCCCCGAAAAGTGCCACCTGACGTC

步骤2：将293FT细胞按照8.0×10⁶个/dish的密度铺板于10cm培养皿中，12ml/dish完全培养基，37℃，5％CO₂培养箱中培养，铺板16～24h。

步骤3：待细胞密度为80％～90％后进行转染。转染前1h，吸去细胞培养液，加入12ml 2％FBS转染培养基。

步骤4：将所述结构基因表达质粒与荧光蛋白报告质粒按照一定的质量比(结构基因表达质粒35L1与35L2与荧光蛋白报告质粒EGFP的质量比为1:0.3:0.5)溶于1.0ml无抗性、无牛血清的培养基中，获得质粒混合物；

步骤5：加入PEI混匀，PEI与质粒DNA混合物质量比为3:1，室温孵育15min，形成PEI-DNA复合物，加入平皿中，放入37℃，5％CO₂培养箱中培养。

步骤6：6h后吸去培养液，加入15ml～20ml新鲜的DMEM完全培养基，37℃，5％CO₂培养箱中培养48～96h。

步骤7：倒掉细胞培养液，平皿中加入3ml PBS，收集细胞并1500rpm，离心10min，弃上清。

步骤8：细胞沉淀中加入5ml PBS再次清洗细胞，1500rpm/min，离心10min，弃上清。

步骤9：向细胞沉淀中加入与细胞沉淀等体积的且含有核酸酶的裂解液，混匀，3～4h混匀一次，37℃裂解16h～24h。

步骤10：取出裂解液，冰浴10min后，4℃，6000g/min离心10min，收集上清，即为制备好的假病毒，于-65℃～-85℃保存。

实施例3应用酶联荧光斑点分析仪检测HPV 35型假病毒使用倍数

步骤1：收集对数生长期的293FT细胞，用血球计数板计数并用DMEM完全培养基稀释细胞的密度为1.5×10⁵个/ml。

步骤2：在96孔板细胞培养板中，每孔加入150μl 293FT细胞，置于37℃，5％CO₂的培养箱中培养16～24h。

步骤3：取假病毒溶液用DMEM完全培养基稀释200倍或者适当倍数，再按照2倍倍比稀释，连续稀释4～8个稀释度。

步骤4：每孔加入50μl假病毒稀释液，每个稀释度设置8孔重复，同时设置空白对照，空白对照为50μl DMEM完全培养基，培养72小时后，用酶联荧光斑点分析仪检测每孔的绿色荧光点数。

步骤5：以稀释倍数的对数值(以10为底数)为X轴，以荧光点数平均值为Y轴，进行线性拟合，根据线性拟合曲线计算荧光点数为500个荧光点时的稀释倍数即为假病毒使用稀释倍数，结果如表1～3和图1～3。

表1 HPV 35型假病毒质粒基因序列优化前结果汇总表

表2 HPV 35型假病毒质粒基因序列优化后op1结果汇总表

表3 HPV 35型假病毒质粒基因序列优化后op2结果汇总表

结果表明：HPV35型假病毒质粒基因序列优化前，即使HPV35型假病毒原倍也达不到检测HPV 35型中和抗体滴度所需的荧光点数为500，采用HPV 35L1-op1+HPV 35L2-op1表达质粒制备HPV 35型假病毒使用稀释倍数为839倍，虽然也能够进行中和滴度检验，但假病毒使用量较大，检验成本较高。采用HPV 35L1-op2+HPV 35L2-op2表达质粒制备HPV 35 型假病毒使用稀释倍数为4046倍，综上所述，HPV35型假病毒质粒基因序列优化前无法用于中和抗体滴度检测。而采用HPV 35L1-op2+HPV 35L2-op2表达质粒制备HPV 35型假病毒质优化后，荧光点数平均值与稀释倍数的对数值成良好的线性关系，并且假病毒使用稀释倍数为4046，因此对HPV 35型中和抗体滴度检测无细胞毒性，中和抗体滴度检测的准确度较好，并且同样制备一批假病毒所能够用于中和抗体滴度检验的样本量更多，故检验成本大幅降低。

实施例4应用假病毒检测HPV 35型血清的中和抗体滴度

步骤1：将293FT细胞按1.5×10⁴/well的密度预铺于96孔细胞培养板中，每孔体积为100μl， 37℃，5％CO₂培养箱中培养至细胞贴壁。

步骤2：将血清样品分别用DMEM完全培养基稀释到320倍，进行连续倍比稀释适当稀释倍数，血清样品为双复孔。

步骤3：取假病毒溶液用DMEM完全培养基稀释至每孔荧光点数为200～1000个，混匀，同时设置阳性对照(假病毒对照)8孔，空白对照(培养基对照)8孔。

步骤4：在60μl血清稀释液中加入60μl假病毒稀释液，振荡混匀1分钟后置于25±2℃恒温培养箱中孵育60±2分钟。

步骤5：准确吸取血清-假病毒混合液100μl，缓慢小心加入96孔预铺的细胞板中。

步骤6：于37℃，5％CO₂培养箱中培养68～76小时后用酶联荧光斑点分析仪观察结果。

步骤7：按下列公式计算Cut-off值：Cut-off值＝假病毒对照荧光点数的平均值÷2

步骤8：血清样品的平均荧光点数≤Cut-off值对应的最大稀释倍数即为样品的中和抗体滴度。

取4份HPV 35血清样本按上述步骤操作，Cut-off值为328，样本1～4的中和抗体滴度 分别为1280、640、320和1280。结果如表4和图4。

表4 HPV 35型假病毒用于中和抗体滴度检验结果汇总表

结果表明：HPV 35假病毒质粒基因序列优化后制备的假病毒稀释839倍后用于中和抗体滴度检验时假病毒对照荧光点数的平均值为656，符合假病毒对照荧光点数为200～1000个，适用于血清样本的中和抗体滴度检验。具有检验成本较低，检验结果准确度较好，重复性好等优点。

序列表

<110> 上海博唯生物科技有限公司

<120> 一种人***瘤病毒35型/HPV35型L1及其制备与应用

<160> 15

<170> SIPOSequenceListing 1.0

<210> 1

<211> 502

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 1

Met Ser Leu Trp Arg Ser Asn Glu Ala Thr Val Tyr Leu Pro Pro Val

1 5 10 15

Ser Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Thr Arg Thr Asn

20 25 30

Ile Tyr Tyr His Ala Gly Ser Ser Arg Leu Leu Ala Val Gly His Pro

35 40 45

Tyr Tyr Ala Ile Lys Lys Gln Asp Ser Asn Lys Ile Ala Val Pro Lys

50 55 60

Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Val Lys Leu Pro Asp Pro

65 70 75 80

Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asp Pro Ala Ser Gln

85 90 95

Arg Leu Val Trp Ala Cys Thr Gly Val Glu Val Gly Arg Gly Gln Pro

100 105 110

Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Leu Asp Asp

115 120 125

Thr Glu Asn Ser Asn Lys Tyr Val Gly Asn Ser Gly Thr Asp Asn Arg

130 135 140

Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile Gly

145 150 155 160

Cys Arg Pro Pro Ile Gly Glu His Trp Gly Lys Gly Thr Pro Cys Asn

165 170 175

Ala Asn Gln Val Lys Ala Gly Glu Cys Pro Pro Leu Glu Leu Leu Asn

180 185 190

Thr Val Leu Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met

195 200 205

Asp Phe Thr Thr Leu Gln Ala Asn Lys Ser Asp Val Pro Leu Asp Ile

210 215 220

Cys Ser Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Lys Met Val Ser Glu

225 230 235 240

Pro Tyr Gly Asp Met Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met Phe

245 250 255

Val Arg His Leu Phe Asn Arg Ala Gly Thr Val Gly Glu Thr Val Pro

260 265 270

Ala Asp Leu Tyr Ile Lys Gly Thr Thr Gly Thr Leu Pro Ser Thr Ser

275 280 285

Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp Ala Gln Ile

290 295 300

Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn Asn Gly

305 310 315 320

Ile Cys Trp Ser Asn Gln Leu Phe Val Thr Val Val Asp Thr Thr Arg

325 330 335

Ser Thr Asn Met Ser Val Cys Ser Ala Val Ser Ser Ser Asp Ser Thr

340 345 350

Tyr Lys Asn Asp Asn Phe Lys Glu Tyr Leu Arg His Gly Glu Glu Tyr

355 360 365

Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr Ala Asp

370 375 380

Val Met Thr Tyr Ile His Ser Met Asn Pro Ser Ile Leu Glu Asp Trp

385 390 395 400

Asn Phe Gly Leu Thr Pro Pro Pro Ser Gly Thr Leu Glu Asp Thr Tyr

405 410 415

Arg Tyr Val Thr Ser Gln Ala Val Thr Cys Gln Lys Pro Ser Ala Pro

420 425 430

Lys Pro Lys Asp Asp Pro Leu Lys Asn Tyr Thr Phe Trp Glu Val Asp

435 440 445

Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu Gly Arg

450 455 460

Lys Phe Leu Leu Gln Ala Gly Leu Lys Ala Arg Pro Asn Phe Arg Leu

465 470 475 480

Gly Lys Arg Ala Ala Pro Ala Ser Thr Ser Lys Lys Ser Ser Thr Lys

485 490 495

Arg Arg Lys Val Lys Ser

500

<210> 2

<211> 469

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 2

Met Arg His Lys Arg Ser Thr Lys Arg Val Lys Arg Ala Ser Ala Thr

1 5 10 15

Gln Leu Tyr Arg Thr Cys Lys Ala Ala Gly Thr Cys Pro Pro Asp Val

20 25 30

Ile Pro Lys Val Glu Gly Asn Thr Val Ala Asp Gln Ile Leu Lys Tyr

35 40 45

Gly Ser Met Ala Val Phe Phe Gly Gly Leu Gly Ile Gly Ser Gly Ser

50 55 60

Gly Thr Gly Gly Arg Ser Gly Tyr Val Pro Leu Gly Thr Thr Pro Pro

65 70 75 80

Thr Ala Ala Thr Asn Ile Pro Ile Arg Pro Pro Val Thr Val Glu Ser

85 90 95

Ile Pro Leu Asp Thr Ile Gly Pro Leu Asp Ser Ser Ile Val Ser Leu

100 105 110

Val Glu Glu Thr Ser Phe Ile Glu Ser Gly Ala Pro Val Val Thr Pro

115 120 125

Arg Val Pro Pro Thr Thr Gly Phe Thr Ile Thr Thr Ser Thr Asp Thr

130 135 140

Thr Pro Ala Ile Leu Asp Val Thr Ser Ile Ser Thr His Asp Asn Pro

145 150 155 160

Thr Phe Thr Asp Pro Ser Val Leu His Pro Pro Thr Pro Ala Glu Thr

165 170 175

Ser Gly His Phe Val Leu Ser Ser Ser Ser Ile Ser Thr His Asn Tyr

180 185 190

Glu Glu Ile Pro Met Asp Thr Phe Ile Val Ser Thr Asp Ser Asn Asn

195 200 205

Ile Thr Asn Ser Thr Pro Ile Pro Gly Ser Arg Pro Thr Thr Arg Leu

210 215 220

Gly Leu Tyr Ser Lys Gly Thr Gln Gln Val Lys Val Val Asp Pro Ala

225 230 235 240

Phe Met Thr Ser Pro Ala Lys Leu Ile Thr Tyr Asp Asn Pro Ala Tyr

245 250 255

Glu Gly Leu Asn Pro Asp Thr Thr Leu Gln Phe Glu His Glu Asp Ile

260 265 270

Ser Leu Ala Pro Asp Pro Asp Phe Met Asp Ile Ile Ala Leu His Arg

275 280 285

Pro Ala Leu Thr Ser Arg Lys Gly Thr Ile Arg Tyr Ser Arg Val Gly

290 295 300

Asn Lys Arg Thr Met His Thr Arg Ser Gly Lys Ala Ile Gly Ala Arg

305 310 315 320

Val His Tyr Tyr Gln Asp Leu Ser Ser Ile Thr Glu Asp Ile Glu Leu

325 330 335

Gln Pro Leu Gln His Val Pro Ser Ser Leu Pro His Thr Thr Val Ser

340 345 350

Thr Ser Leu Asn Asp Gly Met Phe Asp Ile Tyr Ala Pro Ile Asp Thr

355 360 365

Glu Glu Asp Ile Ile Phe Ser Ala Ser Ser Asn Asn Thr Leu Tyr Thr

370 375 380

Thr Ser Asn Thr Ala Tyr Val Pro Ser Asn Thr Thr Ile Pro Leu Ser

385 390 395 400

Ser Gly Tyr Asp Ile Pro Ile Thr Ala Gly Pro Asp Ile Val Phe Asn

405 410 415

Ser Asn Thr Ile Thr Asn Thr Val Leu Pro Val Pro Thr Gly Pro Ile

420 425 430

Tyr Ser Ile Ile Ala Asp Gly Gly Asp Phe Tyr Leu His Pro Ser Tyr

435 440 445

Tyr Leu Leu Lys Arg Arg Arg Lys Arg Ile Pro Tyr Phe Phe Ala Asp

450 455 460

Val Ser Val Ala Val

465

<210> 3

<211> 1506

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 3

atgtctctgt ggagatccaa cgaggccact gtctacctgc ctccagtttc ggtgtctaag 60

gttgtgtcca cggacgagta cgtcactaga accaacatct actaccacgc aggttcctct 120

agactcctgg ctgttggtca cccttactat gccattaaga agcaggactc gaacaagatc 180

gccgtcccaa aggtttctgg cttgcagtac agagtgttca gagttaagct gccagaccct 240

aacaagttcg gatttccaga cacctccttc tacgaccctg cttcccagag attggtttgg 300

gcatgcactg gagtcgaggt gggcagaggt cagccattgg gagttggtat ctctggccac 360

cctttgctga acaagctcga cgataccgag aactccaaca agtacgttgg caactctgga 420

accgacaaca gagagtgcat ctcgatggac tacaagcaga cccagttgtg tctcatcgga 480

tgcagaccac ctattggtga acattgggga aagggcaccc cttgcaacgc caaccaggtc 540

aaggccggag agtgtcctcc attggagctt ctgaacactg ttctccaaga tggtgacatg 600

gttgacaccg gctttggtgc tatggacttc acgaccttgc aggccaacaa gtccgacgtg 660

ccacttgaca tctgttcttc catttgcaag taccctgatt acctgaagat ggtttcggag 720

ccatacggag acatgctctt cttttacctg agaagagagc agatgttcgt gagacacttg 780

ttcaacagag caggaactgt tggtgaaacg gtccctgctg acctgtacat caagggcacc 840

actggtacgt tgccatctac ctcgtacttc cctactccat ctggttcgat ggtcacctcc 900

gatgcccaga tcttcaacaa gccatactgg ttgcagagag cccagggaca caacaatggc 960

atttgctggt ccaaccagct gttcgtgacc gtcgttgaca ctacgagatc caccaacatg 1020

tcggtgtgtt ctgcagtcag ctcttccgac tcgacctaca agaacgacaa cttcaaggag 1080

tacctcagac acggtgaaga gtacgacctg caattcatct tccagttgtg caagatcacc 1140

ctgactgctg acgttatgac gtacattcac tccatgaacc cttcgatcct ggaggactgg 1200

aacttcggtc ttactccacc tccatctggc accttggagg acacttacag atatgtcacc 1260

tcccaagctg ttacgtgtca gaagccttcg gccccaaagc ctaaggacga tccactgaag 1320

aactacacct tctgggaggt tgacctgaag gagaagttct ccgcagacct cgaccagttc 1380

ccattgggca gaaagttcct gctccaagct ggattgaagg ccagacctaa cttcagactt 1440

ggcaagagag ccgctccagc atctacctct aagaaatcgt ccacgaagcg cagaaaggtg 1500

aagtcg 1506

<210> 4

<211> 1506

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 4

atgagcctgt ggagaagcaa cgaggccacc gtgtacctgc ccccggtgag cgtgagcaag 60

gtggtgagca ccgacgagta cgtgacaaga accaacatct actaccacgc cggcagcagc 120

agactgctgg ccgtgggcca cccctactac gccatcaaga agcaagacag caacaagatc 180

gccgtgccca aggtgagcgg cctgcagtac agagtgttca gagtgaagct gcccgacccc 240

aacaagttcg gcttccccga cactagcttc tatgacccgg ctagccaaag actggtgtgg 300

gcgtgtacgg gcgtggaggt aggtagaggg cagccactgg gcgtgggcat cagcggccac 360

cccctgctga acaagctgga cgacaccgag aacagcaaca agtacgtggg caacagcggc 420

accgacaaca gagagtgcat cagcatggac tacaagcaga cacagctgtg cctgatcggc 480

tgcagacccc ccatcggcga gcactggggc aaaggcaccc cgtgtaacgc taaccaagtc 540

aaggcgggag agtgcccccc cctggagctg ctgaacaccg tgctgcaaga cggcgacatg 600

gtggacaccg gcttcggcgc catggacttc accaccctgc aagccaacaa gagcgacgtg 660

cccctggaca tctgcagcag catctgcaag taccccgact acctgaagat ggtgagcgag 720

ccctacggcg acatgctgtt cttctacctg agaagagagc agatgttcgt gagacacctg 780

ttcaacagag ccggcaccgt gggcgagacc gtgcccgccg acctgtacat caagggcacc 840

accggcaccc tgcctagcac aagctacttc cccacgccta gcggcagcat ggtgacaagc 900

gacgctcaga tcttcaacaa gccctactgg ctgcagagag cccaaggcca caacaacggc 960

atctgctgga gcaatcagct gttcgtgacc gtggtggaca ccacaagaag caccaacatg 1020

agcgtgtgca gcgccgtgag cagcagcgac agcacctaca agaacgacaa cttcaaggag 1080

tacctgagac acggcgagga gtacgacctg cagttcatct ttcagctgtg caagatcacc 1140

ctgaccgccg acgtgatgac ctacatccac agcatgaacc ctagcatcct ggaggactgg 1200

aacttcggcc tgaccccccc tcctagcggc accctggagg acacctacag atacgtgaca 1260

agccaagccg tgacctgtca gaagcctagc gcccccaagc ccaaggacga ccccctgaag 1320

aactacacct tctgggaggt ggacctgaag gagaagttca gcgccgacct ggatcagttc 1380

cccctgggca gaaagttcct gctgcaagcc ggcctgaagg ctagacccaa cttcagactg 1440

ggcaagagag ccgcccccgc tagcacaagc aagaagagca gcaccaagag aagaaaggtg 1500

aagagc 1506

<210> 5

<211> 1506

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 5

atgagcctgt ggagaagcaa tgaggccaca gtgtacctgc cccctgtgtc tgtgagcaag 60

gtggtgagca cagatgagta tgtgacaaga accaacatct actaccatgc tggcagcagc 120

agactgctgg ctgtgggcca cccctactat gccatcaaga agcaagacag caacaagatt 180

gctgtgccca aggtgtctgg cctgcagtac agagtgttca gagtgaagct gcctgacccc 240

aacaagtttg gcttccctga cactagcttc tatgaccctg ctagccaaag actggtgtgg 300

gcctgtactg gggtggaggt aggtagaggg cagccactgg gggtgggcat ctctggccac 360

cccctgctga acaagctgga tgacacagag aacagcaaca agtatgtggg caactctggc 420

acagacaaca gagagtgcat cagcatggac tacaagcaga cacagctgtg cctgattggc 480

tgcagacccc ccattgggga gcactggggc aaaggcaccc cctgtaatgc caaccaagtc 540

aaggctggag agtgcccccc cctggagctg ctgaacacag tgctgcaaga tggggacatg 600

gtggacactg gctttggggc catggacttc accaccctgc aagccaacaa gtctgatgtg 660

cccctggaca tctgcagcag catctgcaag taccctgact acctgaagat ggtgtctgag 720

ccctatgggg acatgctgtt cttctacctg agaagagagc agatgtttgt gagacacctg 780

ttcaacagag ctggcacagt gggggagaca gtgcctgctg acctgtacat caagggcacc 840

actggcaccc tgcctagcac aagctacttc cccaccccct ctggcagcat ggtgacctct 900

gatgctcaga tcttcaacaa gccctactgg ctgcagagag cccaaggcca caacaatggc 960

atctgctgga gcaatcagct gtttgtgaca gtggtggaca ccacaagaag caccaacatg 1020

tctgtgtgct ctgctgtgag cagctctgac agcacctaca agaatgacaa cttcaaggag 1080

tacctgagac atggggagga gtatgacctg cagttcatct ttcagctgtg caagatcacc 1140

ctgacagctg atgtgatgac ctacatccac agcatgaacc ctagcatcct ggaggactgg 1200

aactttggcc tgaccccccc tccctctggc accctggagg acacctacag atatgtgaca 1260

agccaagctg tgacctgtca gaagccctct gcccccaagc ccaaggatga ccccctgaag 1320

aactacacct tctgggaggt ggacctgaag gagaagttct ctgctgacct ggatcagttc 1380

cccctgggca gaaagttcct gctgcaagct ggcctgaagg ctagacccaa cttcagactg 1440

ggcaagagag ctgcccctgc tagcacaagc aagaagagca gcaccaagag aagaaaggtg 1500

aagagc 1506

<210> 6

<211> 1407

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 6

atgcgccaca agcggtccac caagagagtg aagagggcct ctgccacaca gctgtacaga 60

acctgcaagg cagcaggaac atgtccacct gacgtgatcc ccaaggtgga gggcaacacc 120

gtggccgatc agatcctgaa gtacggctct atggccgtgt tctttggcgg cctgggcatc 180

ggatctggaa gcggaaccgg cggccggagc ggctatgtgc cactgggaac cacaccacca 240

acagcagcaa ccaatatccc catccggcct ccagtgacag tggagtccat ccccctggac 300

accatcggcc ctctggatag ctccatcgtg tctctggtgg aggagacatc cttcatcgag 360

tctggagcac ctgtggtgac cccaagggtg ccacctacca caggctttac aatcaccaca 420

tccaccgaca ccacacctgc catcctggat gtgacatcca tctctaccca cgacaaccca 480

accttcacag atccatctgt gctgcaccca ccaacaccag cagagaccag cggccacttt 540

gtgctgtcta gctcctctat ctccacccac aactacgagg agatccctat ggacacattc 600

atcgtgtcta ccgatagcaa caatatcaca aatagcaccc ctatcccagg ctccagacct 660

accacaaggc tgggcctgta tagcaagggc acccagcagg tgaaggtggt ggacccagcc 720

tttatgacat cccccgccaa gctgatcacc tacgataacc ccgcctatga gggcctgaat 780

ccagacacca cactgcagtt cgagcacgag gatatctccc tggcccccga ccctgacttc 840

atggacatca tcgccctgca cagaccagcc ctgacaagca ggaagggcac catccgctac 900

tcccgggtgg gcaacaagcg cacaatgcac acccggagcg gcaaggcaat cggagcaagg 960

gtgcactact atcaggacct gagctccatc accgaggata tcgagctgca gccactgcag 1020

cacgtgccct ctagcctgcc tcacaccaca gtgagcacat ccctgaatga cggcatgttc 1080

gatatctacg cccccatcga caccgaggag gatatcatct tttccgcctc ctctaacaat 1140

acactgtaca ccacatctaa caccgcctat gtgcctagca ataccacaat cccactgagc 1200

tccggctatg acatccctat cacagccggc ccagatatcg tgttcaacag caataccatc 1260

acaaataccg tgctgccagt gccaaccgga ccaatctact ctatcatcgc agacggaggc 1320

gacttctacc tgcaccctag ctactatctg ctgaagcgga gaaggaagcg gatcccatat 1380

ttctttgccg acgtgagcgt ggccgtg 1407

<210> 7

<211> 1407

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 7

atgagacaca agagaagcac caagagagtg aagagagcta gcgccacaca gctgtacaga 60

acctgcaagg ccgccggcac ctgccccccc gacgtgatcc ccaaggtgga gggcaacacc 120

gtggccgatc agatcctgaa gtatggcagc atggctgtct tcttcggcgg cctgggtatc 180

ggcagcggaa gcggcaccgg cggcagaagt gggtatgtgc cccttggtac gacacccccc 240

accgccgcca ccaacatccc catcagaccc cccgtgaccg tggagagcat ccccctggac 300

accatcggcc ccctggacag cagcatcgtg agcctggtgg aggagactag cttcatcgag 360

agcggcgccc ccgtggtgac ccctagagtg ccccccacca ccggcttcac catcaccaca 420

agcacggaca cgacacccgc catcctggac gtgacctcca tcagcactca cgataaccct 480

acctttaccg accctagcgt tctgcatccc cccacgcccg ccgagacaag cggccacttc 540

gtgctgagca gcagcagcat cagcacccac aactatgagg agatccccat ggacaccttc 600

atcgtgagca ccgacagcaa caacatcaca aactccacac ccattcccgg cagcagaccc 660

accacaagac tgggcctgta cagcaagggc acacagcaag tgaaggtggt ggaccccgcc 720

ttcatgacaa gccccgccaa gctgatcacc tacgacaacc ccgcctacga gggcctgaac 780

cccgacacaa cgctgcagtt cgagcacgag gacatcagcc tggcccccga ccccgacttc 840

atggacatca tcgccctgca cagacccgcc ctgacaagca gaaagggcac catcagatac 900

agcagagtgg gcaacaagag aaccatgcac acaagaagcg gcaaggccat cggcgctaga 960

gtgcactact accaagacct gagcagcatc accgaggaca tcgagctgca gcccctgcag 1020

cacgttccct cgtccctgcc ccacaccacc gtgagcacaa gcctgaacga cggcatgttc 1080

gacatctacg cccccatcga caccgaggag gacatcatct tcagcgctag cagcaacaac 1140

accctgtaca ccacaagcaa caccgcctac gtgcctagca acaccaccat ccccctgagc 1200

agcggctacg acatccccat aactgccggc cccgacatcg tgttcaacag caacaccatc 1260

accaacaccg tgctgcccgt gcccaccggc cccatctaca gcatcatcgc cgacggcggc 1320

gacttctacc tgcaccctag ctactacctg ctgaagagaa gaagaaagag aatcccctac 1380

ttcttcgccg acgtgagcgt ggccgtg 1407

<210> 8

<211> 1407

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 8

atgagacaca agagaagcac caagagagtg aagagagcct ctgccacaca gctgtacaga 60

acctgcaagg ctgctggcac ctgcccccct gatgtgatcc ccaaggtgga gggcaacaca 120

gtggctgatc agatcctgaa gtatggcagc atggctgtct tctttggggg cctgggtatt 180

ggctctggct ctggcactgg gggcagaagt gggtatgtgc cccttggtac cacacccccc 240

acagctgcca ccaacatccc catcagaccc cctgtgacag tggagagcat ccccctggac 300

accattggcc ccctggacag cagcattgtg agcctggtgg aggagactag cttcattgag 360

tctggggccc ctgtggtgac ccctagagtg ccccccacca ctggcttcac catcaccaca 420

agcacagaca ccacacctgc catcctggat gtgacctcca tcagcactca tgacaaccct 480

acctttacag acccctctgt gctgcatccc cccacccctg ctgagacctc tggccacttt 540

gtgctgagca gcagcagcat cagcacccac aactatgagg agatccccat ggacaccttc 600

attgtgagca cagacagcaa caacatcaca aactccacac ccattcctgg cagcagaccc 660

accacaagac tgggcctgta cagcaagggc acacagcaag tgaaggtggt ggaccctgcc 720

ttcatgacaa gccctgccaa gctgatcacc tatgacaacc ctgcctatga gggcctgaac 780

cctgacacaa ccctgcagtt tgagcatgag gacatcagcc tggcccctga ccctgacttc 840

atggacatca ttgccctgca cagacctgcc ctgacaagca gaaagggcac catcagatac 900

agcagagtgg gcaacaagag aaccatgcac acaagatctg gcaaggccat tggggctaga 960

gtgcactact accaagacct gagcagcatc acagaggaca ttgagctgca gcccctgcag 1020

catgtgccta gctccctgcc ccacaccaca gtgagcacaa gcctgaatga tggcatgttt 1080

gacatctatg cccccattga cacagaggag gacatcatct tctctgctag cagcaacaac 1140

accctgtaca ccacaagcaa cacagcctat gtgcctagca acaccaccat ccccctgagc 1200

tctggctatg acatccccat aactgctggc cctgacattg tgttcaacag caacaccatc 1260

accaacacag tgctgcctgt gcccactggc cccatctaca gcatcattgc tgatgggggg 1320

gacttctacc tgcaccctag ctactacctg ctgaagagaa gaagaaagag aatcccctac 1380

ttctttgctg atgtgtctgt ggctgtg 1407

<210> 9

<211> 6882

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 9

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg tctctgtgga gatccaacga 960

ggccactgtc tacctgcctc cagtttcggt gtctaaggtt gtgtccacgg acgagtacgt 1020

cactagaacc aacatctact accacgcagg ttcctctaga ctcctggctg ttggtcaccc 1080

ttactatgcc attaagaagc aggactcgaa caagatcgcc gtcccaaagg tttctggctt 1140

gcagtacaga gtgttcagag ttaagctgcc agaccctaac aagttcggat ttccagacac 1200

ctccttctac gaccctgctt cccagagatt ggtttgggca tgcactggag tcgaggtggg 1260

cagaggtcag ccattgggag ttggtatctc tggccaccct ttgctgaaca agctcgacga 1320

taccgagaac tccaacaagt acgttggcaa ctctggaacc gacaacagag agtgcatctc 1380

gatggactac aagcagaccc agttgtgtct catcggatgc agaccaccta ttggtgaaca 1440

ttggggaaag ggcacccctt gcaacgccaa ccaggtcaag gccggagagt gtcctccatt 1500

ggagcttctg aacactgttc tccaagatgg tgacatggtt gacaccggct ttggtgctat 1560

ggacttcacg accttgcagg ccaacaagtc cgacgtgcca cttgacatct gttcttccat 1620

ttgcaagtac cctgattacc tgaagatggt ttcggagcca tacggagaca tgctcttctt 1680

ttacctgaga agagagcaga tgttcgtgag acacttgttc aacagagcag gaactgttgg 1740

tgaaacggtc cctgctgacc tgtacatcaa gggcaccact ggtacgttgc catctacctc 1800

gtacttccct actccatctg gttcgatggt cacctccgat gcccagatct tcaacaagcc 1860

atactggttg cagagagccc agggacacaa caatggcatt tgctggtcca accagctgtt 1920

cgtgaccgtc gttgacacta cgagatccac caacatgtcg gtgtgttctg cagtcagctc 1980

ttccgactcg acctacaaga acgacaactt caaggagtac ctcagacacg gtgaagagta 2040

cgacctgcaa ttcatcttcc agttgtgcaa gatcaccctg actgctgacg ttatgacgta 2100

cattcactcc atgaaccctt cgatcctgga ggactggaac ttcggtctta ctccacctcc 2160

atctggcacc ttggaggaca cttacagata tgtcacctcc caagctgtta cgtgtcagaa 2220

gccttcggcc ccaaagccta aggacgatcc actgaagaac tacaccttct gggaggttga 2280

cctgaaggag aagttctccg cagacctcga ccagttccca ttgggcagaa agttcctgct 2340

ccaagctgga ttgaaggcca gacctaactt cagacttggc aagagagccg ctccagcatc 2400

tacctctaag aaatcgtcca cgaagcgcag aaaggtgaag tcgtaataga agcttaagtt 2460

taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 2520

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 2580

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 2640

aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 2700

ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 2760

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 2820

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 2880

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 2940

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3000

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3060

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3120

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3180

aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 3240

aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3300

cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3360

cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3420

ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 3480

gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 3540

tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3600

agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3660

ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3720

cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 3780

agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3840

cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 3900

atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3960

tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4020

acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4080

gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 4140

cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4200

tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4260

tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4320

cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4380

ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4440

gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4500

gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 4560

ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4620

aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 4680

catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 4740

cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 4800

tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 4860

cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 4920

gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 4980

tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 5040

acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 5100

aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 5160

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 5220

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 5280

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 5340

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 5400

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 5460

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 5520

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 5580

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 5640

ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga 5700

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5760

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5820

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5880

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5940

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6000

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6060

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6120

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6180

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6240

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6300

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6360

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6420

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6480

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6540

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6600

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6660

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6720

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6780

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6840

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc 6882

<210> 10

<211> 6882

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 10

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg agcctgtgga gaagcaacga 960

ggccaccgtg tacctgcccc cggtgagcgt gagcaaggtg gtgagcaccg acgagtacgt 1020

gacaagaacc aacatctact accacgccgg cagcagcaga ctgctggccg tgggccaccc 1080

ctactacgcc atcaagaagc aagacagcaa caagatcgcc gtgcccaagg tgagcggcct 1140

gcagtacaga gtgttcagag tgaagctgcc cgaccccaac aagttcggct tccccgacac 1200

tagcttctat gacccggcta gccaaagact ggtgtgggcg tgtacgggcg tggaggtagg 1260

tagagggcag ccactgggcg tgggcatcag cggccacccc ctgctgaaca agctggacga 1320

caccgagaac agcaacaagt acgtgggcaa cagcggcacc gacaacagag agtgcatcag 1380

catggactac aagcagacac agctgtgcct gatcggctgc agacccccca tcggcgagca 1440

ctggggcaaa ggcaccccgt gtaacgctaa ccaagtcaag gcgggagagt gcccccccct 1500

ggagctgctg aacaccgtgc tgcaagacgg cgacatggtg gacaccggct tcggcgccat 1560

ggacttcacc accctgcaag ccaacaagag cgacgtgccc ctggacatct gcagcagcat 1620

ctgcaagtac cccgactacc tgaagatggt gagcgagccc tacggcgaca tgctgttctt 1680

ctacctgaga agagagcaga tgttcgtgag acacctgttc aacagagccg gcaccgtggg 1740

cgagaccgtg cccgccgacc tgtacatcaa gggcaccacc ggcaccctgc ctagcacaag 1800

ctacttcccc acgcctagcg gcagcatggt gacaagcgac gctcagatct tcaacaagcc 1860

ctactggctg cagagagccc aaggccacaa caacggcatc tgctggagca atcagctgtt 1920

cgtgaccgtg gtggacacca caagaagcac caacatgagc gtgtgcagcg ccgtgagcag 1980

cagcgacagc acctacaaga acgacaactt caaggagtac ctgagacacg gcgaggagta 2040

cgacctgcag ttcatctttc agctgtgcaa gatcaccctg accgccgacg tgatgaccta 2100

catccacagc atgaacccta gcatcctgga ggactggaac ttcggcctga ccccccctcc 2160

tagcggcacc ctggaggaca cctacagata cgtgacaagc caagccgtga cctgtcagaa 2220

gcctagcgcc cccaagccca aggacgaccc cctgaagaac tacaccttct gggaggtgga 2280

cctgaaggag aagttcagcg ccgacctgga tcagttcccc ctgggcagaa agttcctgct 2340

gcaagccggc ctgaaggcta gacccaactt cagactgggc aagagagccg cccccgctag 2400

cacaagcaag aagagcagca ccaagagaag aaaggtgaag agctaataga agcttaagtt 2460

taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 2520

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 2580

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 2640

aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 2700

ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 2760

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 2820

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 2880

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 2940

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3000

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3060

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3120

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3180

aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 3240

aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3300

cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3360

cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3420

ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 3480

gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 3540

tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3600

agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3660

ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3720

cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 3780

agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3840

cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 3900

atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3960

tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4020

acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4080

gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 4140

cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4200

tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4260

tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4320

cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4380

ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4440

gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4500

gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 4560

ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4620

aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 4680

catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 4740

cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 4800

tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 4860

cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 4920

gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 4980

tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 5040

acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 5100

aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 5160

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 5220

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 5280

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 5340

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 5400

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 5460

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 5520

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 5580

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 5640

ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga 5700

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5760

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5820

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5880

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5940

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6000

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6060

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6120

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6180

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6240

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6300

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6360

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6420

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6480

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6540

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6600

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6660

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6720

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6780

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6840

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc 6882

<210> 11

<211> 6882

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 11

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg agcctgtgga gaagcaatga 960

ggccacagtg tacctgcccc ctgtgtctgt gagcaaggtg gtgagcacag atgagtatgt 1020

gacaagaacc aacatctact accatgctgg cagcagcaga ctgctggctg tgggccaccc 1080

ctactatgcc atcaagaagc aagacagcaa caagattgct gtgcccaagg tgtctggcct 1140

gcagtacaga gtgttcagag tgaagctgcc tgaccccaac aagtttggct tccctgacac 1200

tagcttctat gaccctgcta gccaaagact ggtgtgggcc tgtactgggg tggaggtagg 1260

tagagggcag ccactggggg tgggcatctc tggccacccc ctgctgaaca agctggatga 1320

cacagagaac agcaacaagt atgtgggcaa ctctggcaca gacaacagag agtgcatcag 1380

catggactac aagcagacac agctgtgcct gattggctgc agacccccca ttggggagca 1440

ctggggcaaa ggcaccccct gtaatgccaa ccaagtcaag gctggagagt gcccccccct 1500

ggagctgctg aacacagtgc tgcaagatgg ggacatggtg gacactggct ttggggccat 1560

ggacttcacc accctgcaag ccaacaagtc tgatgtgccc ctggacatct gcagcagcat 1620

ctgcaagtac cctgactacc tgaagatggt gtctgagccc tatggggaca tgctgttctt 1680

ctacctgaga agagagcaga tgtttgtgag acacctgttc aacagagctg gcacagtggg 1740

ggagacagtg cctgctgacc tgtacatcaa gggcaccact ggcaccctgc ctagcacaag 1800

ctacttcccc accccctctg gcagcatggt gacctctgat gctcagatct tcaacaagcc 1860

ctactggctg cagagagccc aaggccacaa caatggcatc tgctggagca atcagctgtt 1920

tgtgacagtg gtggacacca caagaagcac caacatgtct gtgtgctctg ctgtgagcag 1980

ctctgacagc acctacaaga atgacaactt caaggagtac ctgagacatg gggaggagta 2040

tgacctgcag ttcatctttc agctgtgcaa gatcaccctg acagctgatg tgatgaccta 2100

catccacagc atgaacccta gcatcctgga ggactggaac tttggcctga ccccccctcc 2160

ctctggcacc ctggaggaca cctacagata tgtgacaagc caagctgtga cctgtcagaa 2220

gccctctgcc cccaagccca aggatgaccc cctgaagaac tacaccttct gggaggtgga 2280

cctgaaggag aagttctctg ctgacctgga tcagttcccc ctgggcagaa agttcctgct 2340

gcaagctggc ctgaaggcta gacccaactt cagactgggc aagagagctg cccctgctag 2400

cacaagcaag aagagcagca ccaagagaag aaaggtgaag agctaataga agcttaagtt 2460

taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 2520

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 2580

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 2640

aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 2700

ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 2760

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 2820

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 2880

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 2940

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3000

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3060

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3120

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3180

aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 3240

aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3300

cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3360

cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3420

ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 3480

gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 3540

tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3600

agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3660

ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3720

cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 3780

agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3840

cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 3900

atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3960

tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4020

acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4080

gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 4140

cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4200

tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4260

tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4320

cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4380

ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4440

gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4500

gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 4560

ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4620

aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 4680

catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 4740

cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 4800

tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 4860

cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 4920

gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 4980

tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 5040

acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 5100

aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 5160

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 5220

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 5280

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 5340

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 5400

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 5460

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 5520

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 5580

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 5640

ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga 5700

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5760

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5820

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5880

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5940

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6000

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6060

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6120

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6180

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6240

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6300

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6360

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6420

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6480

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6540

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6600

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6660

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6720

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6780

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6840

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc 6882

<210> 12

<211> 6783

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 12

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg cgccacaagc ggtccaccaa 960

gagagtgaag agggcctctg ccacacagct gtacagaacc tgcaaggcag caggaacatg 1020

tccacctgac gtgatcccca aggtggaggg caacaccgtg gccgatcaga tcctgaagta 1080

cggctctatg gccgtgttct ttggcggcct gggcatcgga tctggaagcg gaaccggcgg 1140

ccggagcggc tatgtgccac tgggaaccac accaccaaca gcagcaacca atatccccat 1200

ccggcctcca gtgacagtgg agtccatccc cctggacacc atcggccctc tggatagctc 1260

catcgtgtct ctggtggagg agacatcctt catcgagtct ggagcacctg tggtgacccc 1320

aagggtgcca cctaccacag gctttacaat caccacatcc accgacacca cacctgccat 1380

cctggatgtg acatccatct ctacccacga caacccaacc ttcacagatc catctgtgct 1440

gcacccacca acaccagcag agaccagcgg ccactttgtg ctgtctagct cctctatctc 1500

cacccacaac tacgaggaga tccctatgga cacattcatc gtgtctaccg atagcaacaa 1560

tatcacaaat agcaccccta tcccaggctc cagacctacc acaaggctgg gcctgtatag 1620

caagggcacc cagcaggtga aggtggtgga cccagccttt atgacatccc ccgccaagct 1680

gatcacctac gataaccccg cctatgaggg cctgaatcca gacaccacac tgcagttcga 1740

gcacgaggat atctccctgg cccccgaccc tgacttcatg gacatcatcg ccctgcacag 1800

accagccctg acaagcagga agggcaccat ccgctactcc cgggtgggca acaagcgcac 1860

aatgcacacc cggagcggca aggcaatcgg agcaagggtg cactactatc aggacctgag 1920

ctccatcacc gaggatatcg agctgcagcc actgcagcac gtgccctcta gcctgcctca 1980

caccacagtg agcacatccc tgaatgacgg catgttcgat atctacgccc ccatcgacac 2040

cgaggaggat atcatctttt ccgcctcctc taacaataca ctgtacacca catctaacac 2100

cgcctatgtg cctagcaata ccacaatccc actgagctcc ggctatgaca tccctatcac 2160

agccggccca gatatcgtgt tcaacagcaa taccatcaca aataccgtgc tgccagtgcc 2220

aaccggacca atctactcta tcatcgcaga cggaggcgac ttctacctgc accctagcta 2280

ctatctgctg aagcggagaa ggaagcggat cccatatttc tttgccgacg tgagcgtggc 2340

cgtgtaatag aagcttaagt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc 2400

agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 2460

ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 2520

ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 2580

atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 2640

gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 2700

gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 2760

cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 2820

ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 2880

cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 2940

tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 3000

cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 3060

aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 3120

cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3180

gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3240

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3300

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3360

ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3420

caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 3480

atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3540

gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3600

ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 3660

gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 3720

cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 3780

gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 3840

tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 3900

gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 3960

tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 4020

catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4080

ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4140

ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4200

tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4260

tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4320

acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4380

ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4440

gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 4500

agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4560

aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 4620

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 4680

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 4740

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 4800

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 4860

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 4920

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 4980

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5040

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5100

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5160

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5220

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5280

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5340

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5400

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 5460

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 5520

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc 5580

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5640

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5700

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5760

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5820

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5880

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5940

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6000

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6060

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6120

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6180

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6240

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6300

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6360

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6420

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6480

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6540

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6600

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6660

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6720

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6780

gtc 6783

<210> 13

<211> 6783

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 13

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg agacacaaga gaagcaccaa 960

gagagtgaag agagctagcg ccacacagct gtacagaacc tgcaaggccg ccggcacctg 1020

cccccccgac gtgatcccca aggtggaggg caacaccgtg gccgatcaga tcctgaagta 1080

tggcagcatg gctgtcttct tcggcggcct gggtatcggc agcggaagcg gcaccggcgg 1140

cagaagtggg tatgtgcccc ttggtacgac accccccacc gccgccacca acatccccat 1200

cagacccccc gtgaccgtgg agagcatccc cctggacacc atcggccccc tggacagcag 1260

catcgtgagc ctggtggagg agactagctt catcgagagc ggcgcccccg tggtgacccc 1320

tagagtgccc cccaccaccg gcttcaccat caccacaagc acggacacga cacccgccat 1380

cctggacgtg acctccatca gcactcacga taaccctacc tttaccgacc ctagcgttct 1440

gcatcccccc acgcccgccg agacaagcgg ccacttcgtg ctgagcagca gcagcatcag 1500

cacccacaac tatgaggaga tccccatgga caccttcatc gtgagcaccg acagcaacaa 1560

catcacaaac tccacaccca ttcccggcag cagacccacc acaagactgg gcctgtacag 1620

caagggcaca cagcaagtga aggtggtgga ccccgccttc atgacaagcc ccgccaagct 1680

gatcacctac gacaaccccg cctacgaggg cctgaacccc gacacaacgc tgcagttcga 1740

gcacgaggac atcagcctgg cccccgaccc cgacttcatg gacatcatcg ccctgcacag 1800

acccgccctg acaagcagaa agggcaccat cagatacagc agagtgggca acaagagaac 1860

catgcacaca agaagcggca aggccatcgg cgctagagtg cactactacc aagacctgag 1920

cagcatcacc gaggacatcg agctgcagcc cctgcagcac gttccctcgt ccctgcccca 1980

caccaccgtg agcacaagcc tgaacgacgg catgttcgac atctacgccc ccatcgacac 2040

cgaggaggac atcatcttca gcgctagcag caacaacacc ctgtacacca caagcaacac 2100

cgcctacgtg cctagcaaca ccaccatccc cctgagcagc ggctacgaca tccccataac 2160

tgccggcccc gacatcgtgt tcaacagcaa caccatcacc aacaccgtgc tgcccgtgcc 2220

caccggcccc atctacagca tcatcgccga cggcggcgac ttctacctgc accctagcta 2280

ctacctgctg aagagaagaa gaaagagaat cccctacttc ttcgccgacg tgagcgtggc 2340

cgtgtaatag aagcttaagt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc 2400

agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 2460

ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 2520

ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 2580

atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 2640

gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 2700

gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 2760

cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 2820

ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 2880

cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 2940

tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 3000

cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 3060

aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 3120

cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3180

gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3240

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3300

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3360

ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3420

caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 3480

atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3540

gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3600

ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 3660

gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 3720

cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 3780

gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 3840

tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 3900

gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 3960

tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 4020

catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4080

ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4140

ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4200

tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4260

tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4320

acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4380

ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4440

gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 4500

agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4560

aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 4620

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 4680

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 4740

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 4800

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 4860

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 4920

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 4980

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5040

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5100

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5160

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5220

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5280

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5340

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5400

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 5460

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 5520

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc 5580

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5640

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5700

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5760

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5820

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5880

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5940

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6000

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6060

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6120

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6180

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6240

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6300

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6360

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6420

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6480

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6540

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6600

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6660

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6720

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6780

gtc 6783

<210> 14

<211> 6783

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 14

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg agacacaaga gaagcaccaa 960

gagagtgaag agagcctctg ccacacagct gtacagaacc tgcaaggctg ctggcacctg 1020

cccccctgat gtgatcccca aggtggaggg caacacagtg gctgatcaga tcctgaagta 1080

tggcagcatg gctgtcttct ttgggggcct gggtattggc tctggctctg gcactggggg 1140

cagaagtggg tatgtgcccc ttggtaccac accccccaca gctgccacca acatccccat 1200

cagaccccct gtgacagtgg agagcatccc cctggacacc attggccccc tggacagcag 1260

cattgtgagc ctggtggagg agactagctt cattgagtct ggggcccctg tggtgacccc 1320

tagagtgccc cccaccactg gcttcaccat caccacaagc acagacacca cacctgccat 1380

cctggatgtg acctccatca gcactcatga caaccctacc tttacagacc cctctgtgct 1440

gcatcccccc acccctgctg agacctctgg ccactttgtg ctgagcagca gcagcatcag 1500

cacccacaac tatgaggaga tccccatgga caccttcatt gtgagcacag acagcaacaa 1560

catcacaaac tccacaccca ttcctggcag cagacccacc acaagactgg gcctgtacag 1620

caagggcaca cagcaagtga aggtggtgga ccctgccttc atgacaagcc ctgccaagct 1680

gatcacctat gacaaccctg cctatgaggg cctgaaccct gacacaaccc tgcagtttga 1740

gcatgaggac atcagcctgg cccctgaccc tgacttcatg gacatcattg ccctgcacag 1800

acctgccctg acaagcagaa agggcaccat cagatacagc agagtgggca acaagagaac 1860

catgcacaca agatctggca aggccattgg ggctagagtg cactactacc aagacctgag 1920

cagcatcaca gaggacattg agctgcagcc cctgcagcat gtgcctagct ccctgcccca 1980

caccacagtg agcacaagcc tgaatgatgg catgtttgac atctatgccc ccattgacac 2040

agaggaggac atcatcttct ctgctagcag caacaacacc ctgtacacca caagcaacac 2100

agcctatgtg cctagcaaca ccaccatccc cctgagctct ggctatgaca tccccataac 2160

tgctggccct gacattgtgt tcaacagcaa caccatcacc aacacagtgc tgcctgtgcc 2220

cactggcccc atctacagca tcattgctga tgggggggac ttctacctgc accctagcta 2280

ctacctgctg aagagaagaa gaaagagaat cccctacttc tttgctgatg tgtctgtggc 2340

tgtgtaatag aagcttaagt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc 2400

agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 2460

ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 2520

ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 2580

atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 2640

gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 2700

gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 2760

cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 2820

ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 2880

cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 2940

tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 3000

cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 3060

aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 3120

cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3180

gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3240

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3300

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3360

ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3420

caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 3480

atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3540

gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3600

ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 3660

gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 3720

cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 3780

gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 3840

tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 3900

gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 3960

tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 4020

catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4080

ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4140

ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4200

tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4260

tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4320

acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4380

ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4440

gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 4500

agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4560

aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 4620

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 4680

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 4740

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 4800

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 4860

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 4920

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 4980

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5040

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5100

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5160

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5220

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5280

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5340

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5400

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 5460

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 5520

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc 5580

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5640

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5700

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5760

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5820

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5880

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5940

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6000

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6060

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6120

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6180

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6240

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6300

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6360

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6420

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6480

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6540

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6600

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6660

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6720

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6780

gtc 6783

<210> 15

<211> 6093

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 15

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg gtgagcaagg gcgaggagct 960

gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 1020

cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 1080

ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 1140

cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 1200

catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 1260

gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 1320

catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 1380

ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 1440

ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 1500

catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 1560

gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 1620

cgggatcact ctcggcatgg acgagctgta caagtaatag aagcttaagt ttaaaccgct 1680

gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 1740

cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 1800

catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 1860

agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 1920

ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 1980

cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 2040

tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 2100

gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 2160

accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 2220

tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 2280

gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 2340

cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 2400

gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 2460

aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 2520

cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 2580

gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 2640

tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 2700

aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 2760

tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 2820

gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 2880

gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 2940

ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 3000

atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 3060

gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 3120

tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 3180

tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 3240

gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 3300

agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 3360

ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 3420

cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 3480

tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 3540

cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 3600

actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 3660

tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 3720

atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 3780

gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 3840

ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 3900

ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 3960

aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 4020

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 4080

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 4140

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 4200

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 4260

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 4320

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 4380

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 4440

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 4500

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 4560

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 4620

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4680

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4740

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 4800

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4860

accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 4920

tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 4980

cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 5040

taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 5100

caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 5160

gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 5220

gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 5280

ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 5340

attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 5400

gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 5460

tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 5520

agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 5580

gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 5640

actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 5700

tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 5760

attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 5820

tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 5880

tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 5940

aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 6000

tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 6060

cgcacatttc cccgaaaagt gccacctgac gtc 6093

Claims (10)

1.一种人***瘤病毒35型L1蛋白和L2蛋白的编码基因，其特征在于，所述L1蛋白编码基因的核苷酸序列如SEQ ID NO.4或SEQ ID NO.5所示；所述L2蛋白编码基因的核苷酸序列如SEQ ID NO.7或SEQ ID NO.8所示。

2.一种重组表达载体，其特征在于，所述重组表达载体中含有如权利要求1所述的编码基因。

3.根据权利要求2所述的重组表达载体，其特征在于，所述重组表达载体中还含有报告基因编码序列。

4.一种宿主细胞，其特征在于，所述宿主细胞中含有如权利要求2所述的重组表达载体。

5.如权利要求1所述的人***瘤病毒35型L1蛋白和L2蛋白的编码基因、如权利要求2所述的重组表达载体、或如权利要求4所述的宿主细胞在制备人***瘤病毒35型假病毒中的用途。

6.一种人***瘤病毒35型假病毒的构建方法，所述方法包括使用核苷酸序列SEQ IDNO.4或SEQ ID NO.5所示的序列编码L1蛋白和核苷酸序列SEQ ID NO.7或SEQ ID NO.8所示的序列编码L2蛋白。

7.根据权利要求6所述的方法，其特征在于，所述方法包括如下步骤:

1)构建包含核苷酸序列SEQ ID NO.4或SEQ ID NO.5所示的序列的人***瘤病毒35型L1蛋白的编码基因的重组表达载体以及包含核苷酸序列SEQ ID NO.7或SEQ ID NO.8所示的序列的人***瘤病毒35型L2蛋白的编码基因的重组表达载体；

2)将两种表达载体在宿主细胞中培养并表达；

3)裂解宿主细胞并收集目的蛋白。

8.根据权利要求7所述的方法，其特征在于，两种所述重组表达载体中还含有报告蛋白表达基因。

9.根据权利要求8所述的方法，其特征在于，所述报告蛋白为荧光蛋白。

10.根据权利要求9所述的方法，其特征在于，所述荧光蛋白选自绿色荧光蛋白、红色荧光蛋白、黄色荧光蛋白、蓝色荧光蛋白的任意一种或几种。

Images